Expression of microbial proteins in plants for production of plants with improved properties

[0001] This application claims priority under 35 U.S.C. § 119 (e) of U.S. Provisional Application No. 60/360,039 filed Feb. 21, 2002, the disclosure of which application is incorporated herein by reference in its entirety.

[0002] Two copies of the sequence listing (Copy 1 and Copy 2) and a computer readable form (CRF) of the sequence listing, all on CD-ROMs, each containing the file named pa_—00433.rpt, which is 136,695,099 bytes (measured in MS-WINDOWS) and was created on Feb. 7, 2003, are herein incorporated by reference.

[0003] Two copies of Tables 1 and 2 on CD-ROMs, each containing the file named pa_—00420.txt, which is 28,959,455 bytes (measured in MS-WINDOWS) and was created on Feb. 18, 2002, are herein incorporated by reference.

[0004] Disclosed herein are inventions in the field of plant biochemistry and genetics. More specifically methods of producing transgenic plants having improved properties as the result of expression of microbial polypeptides are provided.

[0005] The ability to develop transgenic plants with improved traits depends in part on the identification of genes that are useful for production of transformed plants for expression of novel polypeptides. Microbial genes are of interest for expression in transgenic plants for a number of reasons. Bacteria and fungi have been well characterized as model organisms. Therefore there is a wide array of knowledge about the biochemical properties of the genes and pathways in these organisms that allows selection of target genes for expression in plants to produce specific effects on pathways of interest and generate transgenic plants having improved phenotypic properties. In addition, there is a great diversity of genes from which to choose in microorganisms. Of particular interest is the ability to express genes in plants that encode proteins that are not subject to the same types of enzymatic feedback as the native plant genes.

[0006] This invention provides recombinant DNA constructs which provide for expression in plant cells of polypeptides encoded by microbial genes. Expression of such polypeptides in transgenic plants leads to plants having improved phenotypic properties and/or improved response to stressful environmental conditions. Of particular interest are recombinant DNA constructs, wherein said constructs comprise a promoter functional in a plant cell, wherein said promoter is positioned to provide for expression of a polynucleotide encoding a polypeptide from a microbial source, wherein said polynucleotide is selected from the group consisting of:

• [0007] (a) a polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:1 through SEQ ID NO:23687;
• [0008] (b) a polynucleotide encoding a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:23688 through SEQ ID NO:47374;
• [0009] (c) a polynucleotide having at least 70% sequence identity to a polynucleotide of (a) or (b);
• [0010] (d) a polynucleotide encoding a polypeptide having at least 80% sequence identity to a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:23688 through SEQ ID NO:47374, wherein said encoded polypeptide is a functional homolog of said polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:23688 through SEQ ID NO:47374.

[0011] Such constructs are useful for production of transgenic plants having at least one improved biological property as the result of expression of a polypeptide using a construct of this invention. Improved properties of interest include yield, disease resistance, growth rate, stress tolerance and others as set forth in more detail herein.

[0012] The present invention also provides a method of improving a biological property of a plant by inserting into cells of said plant a recombinant DNA construct of the present invention.

[0013] This invention also provides transformed plants, preferably transformed crop plants, comprising a recombinant DNA construct of the present invention, and having an improved biological property as the result of the expression of a microbial polypeptide from said recombinant DNA construct.

[0014] The present invention provides recombinant DNA constructs, wherein said constructs comprise a promoter functional in a plant cell, positioned to provide for expression of a polynucleotide encoding a polypeptide from a microbial source. Microbial polypeptides of interest for expression from such constructs are provided herein and are selected for their ability to impart improved properties to transformed plants as the result of modification of any one or more of a variety of plant phenotypes.

[0015] The constructs of the present invention find use in generation of transgenic plants to provide for expression of polypeptides encoded by polynucleotides, including the native microbial polynucleotides described herein. As a result of such biotechnological applications, plants, particularly crop plants, having improved properties are obtained. Crop plants of interest in the present invention include, but are not limited to soy, cotton, canola, maize, wheat, sunflower, sorghum, alfalfa, barley, millet, rice, tobacco, fruit and vegetable crops, and turf grass. Of particular interest are expression of the disclosed polypeptides to provide plants having improved yield resulting from improved utilization of key biochemical compounds, such as nitrogen, phosphorous or carbohydrate, or resulting from improved responses to environmental stresses, such as cold, heat, drought or salt, or improved response to attack by pests or pathogens. Constructs of the present invention may also be used to provide plants having improved growth and development, and ultimately increased yield, as the result of modified expression of growth regulators or modification of cell cycle or photosynthesis pathways. Other traits of interest that may be modified in plants using constructs of the present invention include seed oil and protein quantity and quality, herbicide tolerance and rate of homologous recombination.

[0016] The polynucleotides or polypeptides from a microbial source as used in this invention may be isolated from the source organism or may be obtained in some other manner, for example by de novo synthesis of polynucleotides. Thus, as used herein, the term “microbial source” indicates that the molecule was identified as naturally existing in a microbe, but does not necessarily indicate the molecule itself was specifically isolated from the source organism.

[0017] As used herein a “transgenic” organism is one whose genome has been altered by the incorporation of foreign genetic material or additional copies of native genetic material, e.g. by transformation or recombination.

[0018] It is understood that the molecules of the invention may be labeled with reagents that facilitate detection of the molecule. As used herein, a label can be any reagent that facilitates detection, including fluorescent labels (Prober, et al., Science 238:336-340 (1987); Albarella et al., EP 144914), chemical labels (Sheldon et al., U.S. Pat. No. 4,582,789; Albarella et al., U.S. Pat. No. 4,563,417), or modified bases (Miyoshi et al., EP 119448), including nucleotides with radioactive elements, e.g. ³²P, ³³P, ³⁵S or ¹²⁵I such as ³²P deoxycytidine-5′-triphosphate (³²PdCTP).

[0019] Polynucleotides are capable of specifically hybridizing to other polynucleotides under certain circumstances. As used herein, two polynucleotides are said to be capable of specifically hybridizing to one another if the two molecules are capable of forming an anti-parallel, double-stranded nucleic acid structure. A nucleic acid molecule is said to be the “complement” of another nucleic acid molecule if the molecules exhibit complete complementarity. As used herein, molecules are said to exhibit “complete complementarity” when every nucleotide in each of the molecules is complementary to the corresponding nucleotide of the other. Two molecules are said to be “minimally complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional “low-stringency” conditions. Similarly, the molecules are said to be “complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional “high-stringency” conditions. Conventional stringency conditions are described by Sambrook et al., Molecular Cloning, A Laboratory Manual, 2nd Ed., Cold Spring Harbor Press, Cold Spring Harbor, N.Y. (1989), and by Haynes et al., Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, D.C. (1985).

[0020] Departures from complete complementarity are therefore permissible, as long as such departures do not completely preclude the capacity of the molecules to form a double-stranded structure. Thus, in order for a nucleic acid molecule to serve as a primer or probe it need only be sufficiently complementary in sequence to be able to form a stable double-stranded structure under the particular solvent and salt concentrations employed. Appropriate stringency conditions which promote DNA hybridization are, for example, 6.0× sodium chloride/sodium citrate (SSC) at about 45° C., followed by a wash of 2.0×SSC at 50° C. Such conditions are known to those skilled in the art and can be found, for example in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. Salt concentration and temperature in the wash step can be adjusted to alter hybridization stringency. For example, conditions may vary from low stringency of about 2.0×SSC at 40° C. to moderately stringent conditions of about 2.0×SSC at 50° C. to high stringency conditions of about 0.2×SSC at 50° C.

[0021] As used herein “sequence identity” refers to the extent to which two optimally aligned polynucleotide or peptide sequences are invariant throughout a window of alignment of components, e.g. nucleotides or amino acids. An “identity fraction” for aligned segments of a test sequence and a reference sequence is the number of identical components which are shared by the two aligned sequences divided by the total number of components in the reference sequence segment, i.e. the entire reference sequence or a smaller defined part of the reference sequence. “Percent identity” is the identity fraction times 100. Comparison of sequences to determine percent identity can be accomplished by a number of well-known methods, including for example by using mathematical algorithms, such as those in the BLAST suite of sequence analysis programs.

[0022] Polynucleotides—This invention utilizes polynucleotides that encode polypeptides identified from a microbial source. The encoded polypeptides may be the complete protein encoded by an identified microbial gene, or may be fragments of the encoded protein. Preferably, polynucleotides utilized herein encode polypeptides constituting a substantial portion of the complete protein, and more preferentially, constituting a sufficient portion of the complete protein to provide the relevant biological activity.

[0023] Of particular interest are polynucleotides that encode polypeptides involved in one or more important biological functions that are common between microbes and plants. Such polynucleotides may be expressed in transgenic plants to produce plants having improved phenotypic properties and/or improved response to stressful environmental conditions. See, for example, Tables 3-21 for a list of improved plant properties and responses and the SEQ ID NOs representing exemplary polynucleotides that may be expressed in transgenic plants to impart such improvements.

[0024] Polynucleotides of the present invention are generally used to impart such biological properties by providing for enhanced protein activity in a transgenic organism, preferably a transgenic plant. Enhanced protein activity is evaluated by reference to a wild type cell or organism and can be determined by direct or indirect measurement. Direct measurement of protein activity might include an analytical assay for the protein, per se, or enzymatic product of protein activity. Indirect assay might include measurement of a property affected by the protein.

[0025] The polynucleotides that find use in this invention represent genes from a variety of bacterial and fungal sources as shown in Table 1. Nucleic acid sequences of polynucleotides for use in the constructs of the present invention are exemplified herein by the native microbial polynucleotide sequences provided as SEQ ID NO: 1 through SEQ ID NO: 23687.

[0026] Also of interest for use in the constructs of the present invention are variants of the polynucleotides provided herein. Such variants may be naturally occurring, including homologous polynucleotides from the same or a different species, or may be non-natural variants, for example polynucleotides synthesized using chemical synthesis methods, or generated using recombinant DNA techniques. Degeneracy of the genetic code provides the possibility to substitute at least one base of the protein encoding sequence of a gene with a different base without causing the amino acid sequence of the polypeptide produced from the gene to be changed. Hence, the DNA utilized in the present invention may also have any base sequence that has been changed from SEQ ID NO: 1 through SEQ ID NO: 23687 by substitution in accordance with degeneracy of the genetic code.

[0027] Also of interest regarding variant sequences for expression of microbial polypeptides in plants is the use of polynucleotides optimized for efficient expression of the encoded polypeptide in plants. For example, the encoding polynucleotides can be synthesized or modified using plant preferred codons for improved expression. It is recognized that all or any part of the encoding sequence may be optimized by synthesis or modification, and that partially optimized polynucleotides are also of interest for expression of microbial polypeptides for modification of plant properties. Codon usage tables may be used to identify plant preferred codons, for example by identifying the codons of highest frequency in the proteins expressed in the largest amount in the particular plant species of interest, and to avoid the use of codons that are rarely found in plants. References describing codon usage include: U.S. Pat. No. 5,500,365, Nakamura et al. (2000) Nucl. Acids Res. 28:292, Perlak et al. (1991) Proc. Natl. Acad. Sci. USA 88:3324-3328, Carels et al. (1998) J. Mol. Evol. 46: 45 and Fennoy et al. (993) Nucl. Acids Res. 21(23):5294. Codon usage tables for a number of plant species are also available from the Department of Plant Gene Research at the Kazusa DNA Research Institute, Japan, for example at www.kazusa.or.jp/codon/.

[0028] Additional sequence modifications are known to enhance gene expression in plant hosts. These include elimination of sequences encoding spurious polyadenylation signals, exon-intron splice site signals, transposon-like repeats, hairpin secondary mRNA structures and other such well-characterized sequences which may be deleterious to gene expression. The G−C content of the sequence may also be adjusted to levels generally used by the target plant host. For example, some microbial genes are very rich (>60%) in adenine (A) and thymine (T) while plant genes are on the order of 45-55% A+T. It is also preferred that regions comprising many consecutive A+T bases or G+C bases are disrupted since these regions are predicted to have a higher likelihood to form hairpin structure due to self-complementarity. Therefore, insertion of heterogeneous base pairs would reduce the likelihood of self-complementary secondary structure formation which are known to inhibit transcription and/or translation in some organisms. In most cases, the adverse effects may be minimized by using sequences which do not contain more than five consecutive A+T or G+C.

[0029] As discussed above, modification of the DNA sequences of wild-type genes and construction of t a completely synthetic gene for a given amino acid sequence are both desirable methods for increasing the expression level of non-plant genes in plant cells. In general, regions with multiple consecutive A+T or G+C nucleotides should be avoided. Codons should be selected avoiding the TA and CG doublets where possible. Codon usage can be normalized against a plant preferred codon usage table and the G+C content preferably adjusted to about 50%. The resulting sequence should also be examined to ensure that there are minimal putative plant polyadenylation signals and ATTTA sequences.

[0030] Polynucleotides for use in constructs of the present invention that are variants of the polynucleotides described herein will generally demonstrate significant identity with the polynucleotides provided herein. Of particular interest are polynucleotide homologs having at least about 60% sequence identity, at least about 70% sequence identity, at least about 80% sequence identity, at least about 85% sequence identity, and more preferably at least about 90%, 95% or even greater, such as 98% or 99% sequence identity with polynucleotide sequences described herein.

[0031] Protein and Polypeptide Molecules—This invention encompasses recombinant DNA expression constructs that provide for production of polypeptides from microbial sources in plants and/or plant cells. Amino acid sequences of the polypeptides of interest for expression using constructs of the present invention are provided herein as SEQ ID NO:23688 through SEQ ID NO:47374.

[0032] As used herein, the term “polypeptide” means an unbranched chain of amino acid residues that are covalently linked by an amide linkage between the carboxyl group of one amino acid and the amino group of another. The term polypeptide can encompass whole proteins (i.e. a functional protein encoded by a particular gene), as well as fragments of proteins. Of particular interest are polypeptides which represent whole proteins or a sufficient portion of the entire protein to impart the relevant biological activity of the protein. The term “protein” also includes molecules consisting of one or more polypeptide chains. Thus, a polypeptide for use in constructs of the present invention may also constitute an entire gene product, but only a portion of a functional oligomeric protein having multiple polypeptide chains.

[0033] Of particular interest for expression from constructs of the present invention are polypeptides involved in one or more important biological properties in plants. Such polypeptides may be produced in transgenic plants to provide plants having improved phenotypic properties and/or improved response to stressful environmental conditions. See, Tables 3-21 for improved plant properties and responses and the SEQ ID NOs for the polypeptides whose expression in transgenic plants is of interest to impart such improvements. A summary of such improved properties and polypeptides of interest is provided below.

[0034] Yield/Nitrogen: Yield improvement by improved nitrogen flow, sensing, uptake, storage and/or transport. Polypeptides useful for imparting such properties include those involved in aspartate and glutamate biosynthesis, polypeptides involved in aspartate and glutamate transport, polypeptides associated with the TOR (Target of Rapamycin) pathway, nitrate transporters, ammonium transporters, chlorate transporters and polypeptides involved in tetrapyrrole biosynthesis.

[0035] Yield/Carbohydrate: Yield improvement by effects on carbohydrate metabolism, for example by increased sucrose production and/or transport. Polypeptides useful for improved yield by effects on carbohydrate metabolism include polypeptides involved in sucrose or starch metabolism, carbon assimilation or carbohydrate transport, including, for example sucrose transporters or glucose/hexose transporters, enzymes involved in glycolysis/gluconeogenesis, the pentose phosphate cycle, or raffinose biosynthesis, and polypeptides involved in glucose signaling, such as SNF1 complex proteins.

[0036] Yield/Photosynthesis: Yield improvement resulting from increased photosynthesis. Polypeptides useful for increasing the rate of photosynthesis include phytochrome, photosystem I and II proteins, electron carriers, ATP synthase, NADH dehydrogenase and cytochrome oxidase.

[0037] Yield/Phosphorus: Yield improvement resulting from increased phosphorus uptake, transport or utilization. Polypeptides useful for improving yield in this manner include phosphatases and phosphate transporters.

[0038] Yield/Stress tolerance: Yield improvement resulting from improved plant growth and development by helping plants to tolerate stressful growth conditions. Polypeptides useful for improved stress tolerance under a variety of stress conditions include polypeptides involved in gene regulation, such as serine/threonine-protein kinases, MAP kinases, MAP kinase kinases, and MAP kinase kinase kinases; polypeptides that act as receptors for signal transduction and regulation, such as receptor protein kinases; intracellular signaling proteins, such as protein phosphatases, GTP binding proteins, and phospholipid signaling proteins; polypeptides involved in arginine biosynthesis; polypeptides involved in ATP metabolism, including for example ATPase, adenylate transporters, and polypeptides involved in ATP synthesis and transport; polypeptides involved in glycine betaine, jasmonic acid, flavonoid or steroid biosynthesis; and homoglobin. Enhanced activity of such polypeptides in transgenic plants will provide changes in the ability of a plant to respond to a variety of environmental stresses, such as chemical stress, drought stress and pest stress.

[0039] Cold tolerance: Polypeptides of interest for improving plant tolerance to cold or freezing temperatures include polypeptides involved in biosynthesis of trehalose or raffinose, polypeptides encoded by cold induced genes, fatty acyl desaturases and other polypeptides involved in glycerolipid or membrane lipid biosynthesis, which find use in modification of membrane fatty acid composition, alternative oxidase, calcium-dependent protein kinases, LEA proteins and uncoupling protein.

[0040] Heat tolerance: Polypeptides of interest for improving plant tolerance to heat include polypeptides involved in biosynthesis of trehalose, polypeptides involved in glycerolipid biosynthesis or membrane lipid metabolism (for altering membrane fatty acid composition), heat shock proteins and mitochondrial NDK.

[0041] Osmotic tolerance: Polypeptides of interest for improving plant tolerance to extreme osmotic conditions include polypeptides involved in proline biosynthesis.

[0042] Drought tolerance: Polypeptides of interest for improving plant tolerance to drought conditions include aquaporins, polypeptides involved in biosynthesis of trehalose or wax, LEA proteins and invertase.

[0043] Pathogen or pest tolerance: Polypeptides of interest for improving plant tolerance to effects of plant pests or pathogens include proteases, polypeptides involved in anthocyanin biosynthesis, polypeptides involved in cell wall metabolism, including cellulases, glucosidases, pectin methylesterase, pectinase, polygalacturonase, chitinase, chitosanase, and cellulose synthase, and polypeptides involved in biosynthesis of terpenoids or indole for production of bioactive metabolites to provide defense against herbivorous insects.

[0044] Cell cycle modification: Polypeptides encoding cell cycle enzymes and regulators of the cell cycle pathway are useful for manipulating growth rate in plants to provide early vigor and accelerated maturation leading to improved yield. Improvements in quality traits, such as seed oil content, may also be obtained by expression of cell cycle enzymes and cell cycle regulators. Polypeptides of interest for modification of cell cycle pathway include cyclins and EIF5alpha pathway proteins, polypeptides involved in polyamine metabolism, polypeptides which act as regulators of the cell cycle pathway, including cyclin-dependent kinases (CDKs), CDK-activating kinases, CDK-inhibitors, Rb and Rb-binding proteins, and transcription factors that activate genes involved in cell proliferation and division, such as the E2F family of transcription factors, proteins involved in degradation of cyclins, such as cullins, and plant homologs of tumor suppressor polypeptides.

[0045] Seed protein yield/content: Polypeptides useful for providing increased seed protein quantity and/or quality include polypeptides involved in the metabolism of amino acids in plants, particularly polypeptides involved in biosynthesis of methionine/cystein and lysine, amino acid transporters, amino acid efflux carriers, seed storage proteins, proteases, and polypeptides involved in phytic acid metabolism.

[0046] Seed oil yield/content: Polypeptides useful for providing increased seed oil quantity and/or quality include polypeptides involved in fatty acid and glycerolipid biosynthesis, beta-oxidation enzymes, enzymes involved in biosynthesis of nutritional compounds, such as carotenoids and tocopherols, and polypeptides that increase embryo size or number or thickness of aleurone.

[0047] Disease response in plants: Polypeptides useful for imparting improved disease responses to plants include polypeptides encoded by cercosporin induced genes, antifungal proteins and proteins encoded by R-genes or SAR genes. Expression of such polypeptides in transgenic plants will provide an increase in disease resistance ability of plants.

[0048] Galactomannanan biosynthesis: Polypeptides involved in production of galactomannans are of interest for providing plants having increased and/or modified reserve polysaccharides for use in food, pharmaceutical, cosmetic, paper and paint industries.

[0049] Flavonoid/isoflavonoid metabolism in plants: Polypeptides of interest for modification of flavonoid/isoflavonoid metabolism in plants include cinnamate-4-hydroxylase, chalcone synthase and flavonol synthase. Enhanced activity of such polypeptides in transgenic plants will provide changes in the quantity and/or speed of flavonoid metabolism in plants and may improve disease resistance by enhancing synthesis of protective secondary metabolites or improving signaling pathways governing disease resistance.

[0050] Growth regulators: Polypeptides involved in production of substances that regulate the growth of various plant tissues are of interest in the present invention and may be used to provide transgenic plants having altered morphologies and improved plant growth and development profiles leading to improvements in yield and stress response. Of particular interest are polypeptides involved in the biosynthesis of plant growth hormones, such as gibberellins, cytokinins, auxins, ethylene and abscisic acid, and other proteins involved in the activity and/or transport of such polypeptides, including for example, cytokinin oxidase, cytokinin/purine permeases, F-box proteins, G-protiens and phytosulfokines.

[0051] Herbicide tolerance: Polypeptides of interest for producing plants having tolerance to plant herbicides include polypeptides involved in the shikimate pathway, which are of interest for providing glyphosate tolerant plants. Such polypeptides include polypeptides involved in biosynthesis of chorismate, phenylalanine, tyrosine and tryptophan.

[0052] Homologous recombination: Increasing the rate of homologous recombination in plants is useful for accelerating the introgression of transgenes into breeding varieties by backcrossing, and to enhance the conventional breeding process by allowing rare recombinants between closely linked genes in phase repulsion to be identified more easily. Polypeptides useful for expression in plants to provide increased homologous recombination include polypeptides involved in mitosis and/or meiosis, including for example, resolvases and polypeptide members of the RAD52 epistasis group.

[0053] Lignin biosynthesis: Polypeptides involved in lignin biosynthesis are of interest for increasing plants' resistance to lodging and for increasing the usefulness of plant materials as biofuels.

[0054] The function of polypeptides used in constructs of the present invention may be known from previous experimental evidence, or may be determined by comparison of the amino acid sequence of the polypeptides to amino acid sequences of other polypeptides for which a function is known. A variety of homology based search algorithms are available to compare a query sequence to a protein database, including for example, BLAST, FASTA, and Smith-Waterman. In the present application, BLASTX and BLASTP algorithms are used to provide protein function information. A number of values are examined in order to assess the confidence of the function assignment. Useful measurements include “E-value” (also shown as “hit_p”), “percent identity”, “percent query coverage”, and “percent hit coverage”.

[0055] In BLAST, E-value, or expectation value, represents the number of different alignments with scores equivalent to or better than the raw alignment score, S, that are expected to occur in a database search by chance. The lower the E value, the more significant the match. Because database size is an element in E-value calculations, E-values obtained by BLASTing against public databases, such as GenBank, have generally increased over time for any given query/entry match. In setting criteria for confidence of polypeptide function prediction, a “high” BLAST match is considered herein as having an E-value for the top BLAST hit provided in Table 1 of less than 1E-30; a medium BLASTX E-value is 1E-30 to 1E-8; and a low BLASTX E-value is greater than 1E-8.

[0056] Percent identity refers to the percentage of identically matched amino acid residues that exist along the length of that portion of the sequences which is aligned by the BLAST algorithm. In setting criteria for confidence of polypeptide function prediction, a “high” BLAST match is considered herein as having percent identity for the top BLAST hit provided in Table 1 of at least 70%; a medium percent identity value is 35% to 70%; and a low percent identity is less than 35%.

[0057] Of particular interest in protein function assignment in the present invention is the use of combinations of E-values, percent identity, query coverage and hit coverage. Query coverage refers to the percent of the query sequence that is represented in the BLAST alignment. Hit coverage refers to the percent of the database entry that is represented in the BLAST alignment. In the present invention, function of a query polypeptide is inferred from function of a protein homolog where either (1) (hit_p<1e-30 or % identity>35%) AND query_coverage>50% AND hit_coverage>50%, or (2) hit_p<1e-8 AND query_coverage>70% AND hit_coverage>70%.

[0058] Functional homologs which differ in one or more amino acids from those of a polypeptide described herein as the result of one or more conservative amino acid substitutions are also of interest for expression in plants using the constructs of the present invention. It is well known in the art that one or more amino acids in a native sequence can be substituted with at least one other amino acid, the charge and polarity of which are similar to that of the native amino acid, resulting in a silent change. For instance, valine is a conservative substitute for alanine and threonine is a conservative substitute for serine. Conservative substitutions for an amino acid within the native polypeptide sequence can be selected from other members of the class to which the naturally occurring amino acid belongs. Amino acids can be divided into the following four groups: (1) acidic amino acids, (2) basic amino acids, (3) neutral polar amino acids, and (4) neutral nonpolar amino acids. Representative amino acids within these various groups include, but are not limited to: (1) acidic (negatively charged) amino acids such as aspartic acid and glutamic acid; (2) basic (positively charged) amino acids such as arginine, histidine, and lysine; (3) neutral polar amino acids such as glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; and (4) neutral nonpolar (hydrophobic) amino acids such as alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine. Conserved substitutes for an amino acid within a native amino acid sequence can be selected from other members of the group to which the naturally occurring amino acid belongs. For example, a group of amino acids having aliphatic side chains is glycine, alanine, valine, leucine, and isoleucine; a group of amino acids having aliphatic-hydroxyl side chains is serine and threonine; a group of amino acids having amide-containing side chains is asparagine and glutamine; a group of amino acids having aromatic side chains is phenylalanine, tyrosine, and tryptophan; a group of amino acids having basic side chains is lysine, arginine, and histidine; and a group of amino acids having sulfur-containing side chains is cysteine and methionine. Naturally conservative amino acids substitution groups are: valine-leucine, valine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alanine-valine, aspartic acid-glutamic acid, and asparagine-glutamine. Also of interest for expression in transgenic plants using constructs as described herein are polypeptides which differ in one or more amino acids from those of a microbial polypeptide described herein as the result of deletion or insertion of one or more amino acids in a native sequence.

[0059] Also of interest for use in constructs of the present invention are functional homologs of the polypeptides described herein which have the same function as a microbial polypeptide provided herein, but with increased or decreased activity or altered specificity. Such variations in protein activity may exist naturally in polypeptides encoded by related genes, for example in a related polypeptide encoded by a different allele or in a different species, or can be achieved by mutagenesis. Naturally occurring variant polypeptides may be obtained by well known nucleic acid or protein screening methods using DNA or antibody probes, for example by screening libraries for genes encoding related polypeptides, or in the case of expression libraries, by screening directly for variant polypeptides. Screening methods for obtaining a modified protein or enzymatic activity of interest by mutagenesis are disclosed in U.S. Pat. No. 5,939,250. An alternative approach to the generation of variants uses random recombination techniques such as “DNA shuffling” as disclosed in U.S. Pat. Nos. 5,605,793; 5,811,238; 5,830,721 and 5,837,458; and International Applications WO 98/31837 and WO 99/65927, all of which are incorporated herein by reference. An alternative method of molecular evolution involves a staggered extension process (StEP) for in vitro mutagenesis and recombination of nucleic acid molecule sequences, as disclosed in U.S. Pat. No. 5,965,408 and International Application WO 98/42832, both of which are incorporated herein by reference.

[0060] Polypeptide variants useful for expression in transgenic plants will generally demonstrate significant identity with the polypeptides described herein. Of particular interest are polypeptides having at least about 35% sequence identity, at least about 50% sequence identity, at least about 60% sequence identity, at least about 70% sequence identity, at least about 80% sequence identity, and more preferably at least about 85%, 90%, 95% or even greater, sequence identity with polypeptide sequences described herein. Of particular interest in the present invention are polypeptides having amino acid sequences provided herein (reference polypeptides) and functional homologs of such reference polypeptides, wherein such functional homologs comprises at least 50 consecutive amino acids having at least 80% identity to a 50 amino acid polypeptide fragment of said reference polypeptide.

[0061] Recombinant DNA Constructs—The present invention encompasses the use of polynucleotides described herein in recombinant constructs, i.e. constructs comprising polynucleotides that are constructed or modified outside of cells and that join nucleic acids that are not found joined in nature. Using methods known to those of ordinary skill in the art, polypeptide encoding sequences of this invention can be inserted into recombinant DNA constructs that can be introduced into a host cell of choice for expression of the encoded protein. Of particular interest in the present invention is the use of the polynucleotides of the present invention for preparation of constructs for use in plant transformation.

[0062] In plant transformation, exogenous genetic material is transferred into a plant cell. By “exogenous” it is meant that a nucleic acid molecule, for example a recombinant DNA construct comprising a polynucleotide of the present invention, is produced outside the organism, e.g. plant, into which it is introduced. An exogenous nucleic acid molecule can have a naturally occurring or non-naturally occurring nucleotide sequence. One skilled in the art recognizes that an exogenous nucleic acid molecule can be derived from the same species into which it is introduced or from a different species. Such exogenous genetic material may be transferred into either monocot or dicot plants including, but not limited to, soy, cotton, canola, maize, teosinte, wheat, rice and Arabidopsis plants. Transformed plant cells comprising such exogenous genetic material may be regenerated to produce whole transformed plants.

[0063] Exogenous genetic material may be transferred into a plant cell by the use of a DNA vector or construct designed for such a purpose. A construct can comprise a number of sequence elements, including promoters, encoding regions, and selectable markers. Vectors are available which have been designed to replicate in both E. coli and A. tumefaciens and have all of the features required for transferring large inserts of DNA into plant chromosomes. Design of such vectors is generally within the skill of the art. See, for example, Plant Molecular Biology: A Laboratory Manual, Clark (ed.), Springier, New York (1997).

[0064] A construct will generally include a plant promoter to direct transcription of the protein encoding region of choice. Numerous promoters that are active in plant cells have been described in the literature. These include the nopaline synthase (NOS) promoter and octopine synthase (OCS) promoters carried on tumor-inducing plasmids of Agrobacterium tumefaciens, caulimovirus promoters such as the cauliflower mosaic virus (CaMV) 19S promoter (Lawton et al., Plant Mol. Biol. 9:315-324 (1987) and 35S promoter (Odell et al., Nature 313:810-812 (1985), CaMV enhanced 35s promoter and the figwort mosaic virus 35S-promoter. Other desirable promoters include the light-inducible promoter from the small subunit of ribulose-1,5-bis-phosphate carboxylase (ssRUBISCO), the actin 1 promoter from rice (McElroy et al. (1991) Mol. Gen. Genet. 231:150-160) or maize (Wang et al. (1992) Molecular and Cellular Biology 12:3399-3406), the Adh promoter (Walker et al., Proc. Natl. Acad. Sci. (U.S.A.) 84:6624-6628 (1987), the sucrose synthase promoter (Yang et al. (1990) Proc. Natl. Acad. Sci. (U.S.A.) 87:4144-4148), the R gene complex promoter (Chandler et al. (1989) The Plant Cell 1:1175-1183), and the chlorophyll a/b binding protein gene promoter. These promoters and numerous others have been used to create DNA constructs for expression in plants. See, for example, PCT publication WO 84/02913. Any promoter known or found to cause transcription of DNA in plant cells can be used in the invention. Other useful promoters are described, for example, in U.S. Pat. Nos. 5,378,619; 5,391,725; 5,428,147; 5,447,858; 5,608,144; 5,608,144; 5,614,399; 5,633,441; 5,633,435; and 4,633,436, all of which are incorporated herein by reference.

[0065] In addition, promoter enhancers, such as the CaMV 35S enhancer (Kay et al. (1987) Science 236:1299-1302) or a tissue specific enhancer (Fromm et al. (1989) The Plant Cell 1:977-984), may be used to enhance gene transcription levels. Enhancers often are found 5′ to the start of transcription in a promoter that functions in eukaryotic cells, but can often be inserted in the forward or reverse orientation 5′ or 3′ to the coding sequence. In some instances, these 5′ enhancing elements are introns. Deemed to be particularly useful as enhancers are the 5′ introns of the rice actin 1 and rice actin 2 genes. Examples of other enhancers which can be used in accordance with the invention include elements from octopine synthase genes (Ellis et al. (1987) EMBO Journal 6:3203-3208), the maize alcohol dehydrogenase gene intron 1 (Callis et al. (1987) Genes and Develop. 1:1183-1200), elements from the maize shrunken 1 gene, the sucrose synthase intron (Vasil et al. (1989) Plant Physiol. 91:1575-1579) and the TMV omega element (Gallie et al. (1989) The Plant Cell 1:301-311), and promoters from non-plant eukaryotes (e.g., yeast; Ruden et al. (1988) Proc Natl. Acad. Sci. 85:4262-4266). DNA constructs can also contain one or more 5′ non-translated leader sequences which serve to enhance polypeptide production from the resulting mRNA transcripts. Such sequences may be derived from the promoter selected to express the gene or can be specifically modified to increase translation of the mRNA. Such regions may also be obtained from viral RNAs, from suitable eukaryotic genes, or from a synthetic gene sequence. For a review of optimizing expression of transgenes, see Koziel et al. (1996) Plant Mol. Biol. 32:393-405).

[0066] Constructs and vectors may also include, with the coding region of interest, a nucleic acid sequence that acts, in whole or in part, to terminate transcription of that region. One type of 3′ untranslated sequence which may be used is a 3′ UTR from the nopaline synthase gene (nos 3′) of Agrobacterium tumefaciens (Bevan et al. (1983) Nucleic Acids Res. 11:369-385). Other 3′ termination regions of interest include those from a gene encoding the small subunit of a ribulose-1,5-bisphosphate carboxylase-oxygenase (rbcS), and more specifically, from a rice rbcS gene (PCT Publication WO 00/70066), the 3′ UTR for the T7 transcript of Agrobacterium tumefaciens (Dhaese et al. (1983) EMBO J 2:419-426), the 3′ end of the protease inhibitor I or II genes from potato (An et al. (1989) Plant Cell 1:115-122) or tomato (Pearce et al. (1991) Science 253:895-898), and the 3′ region isolated from Cauliflower Mosaic Virus (Timmermans et al. (1990) J Biotechnol 14:333-344). Alternatively, one also can use a gamma coixin, oleosin 3 or other 3′ UTRs from the genus Coix (PCT Publication WO 99/58659).

[0067] Constructs and vectors may also include a selectable marker. Selectable markers may be used to select for plants or plant cells that contain the exogenous genetic material. Examples of such include, but are not limited to, a nptII gene (Potrykus et al. (1985) Mol. Gen. Genet. 199:183-188) which codes for kanamycin resistance and can be selected for using kanamycin, G418, etc.; a bar gene which codes for bialaphos resistance; a mutant EPSP synthase gene (Hinchee et al. (1988) Bio/Technology 6:915-922) which encodes glyphosate resistance; a nitrilase gene which confers resistance to bromoxynil (Stalker et al. (1988) J. Biol. Chem. 263:6310-6314); a mutant acetolactate synthase gene (ALS) which confers imidazolinone or sulphonylurea resistance (European Patent Application 154,204 (Sep. 11, 1985)); and a methotrexate resistant DHFR gene (Thillet et al. (1988) J. Biol. Chem. 263:12500-12508.

[0068] Constructs and vectors may also include a screenable marker. Screenable markers may be used to monitor transformation. Exemplary screenable markers include a β-glucuronidase or uidA gene (GUS) which encodes an enzyme for which various chromogenic substrates are known (Jefferson (1987) Plant Mol. Biol, Rep. 5:387-405); Jefferson et al. (1987) EMBO J. 6:3901-3907); an R-locus gene, which encodes a product that regulates the production of anthocyanin pigments (red color) in plant tissues (Dellaporta et al. (1988) Stadler Symposium 11:263-282); Other possible selectable and/or screenable marker genes will be apparent to those of skill in the art.

[0069] Constructs and vectors may also include a transit peptide for targeting of a gene target to a plant organelle, particularly to a chloroplast, leucoplast or other plastid organelle (European Patent Application Publication Number 0218571).

[0070] For use in Agrobacterium mediated transformation methods, constructs of the present invention will also include T-DNA border regions flanking the DNA to be inserted into the plant genome to provide for transfer of the DNA into the plant host chromosome as discussed in more detail below. An exemplary plasmid that finds use in such transformation methods is pCGN8640, a T-DNA vector that can be used to clone exogenous genes and transfer them into plants using Agrobacterium-mediated transformation. pCGN8640 has the restriction sites BamH1, Not1, HindIII, PstI, and SacI positioned between a 35S promoter element and a transcription terminator. Flanking this DNA are the left border and right border sequences necessary for Agrobacterium transformation. The plasmid also has origins of replication for maintaining the plasmid in both E. coli and Agrobacterium tumefaciens strains. A spectinomycin resistance gene on the plasmid can be used to select for the presence of the plasmid in both E. coli and Agrobacterium tumefaciens.

[0071] A candidate gene is prepared for insertion into the T-DNA vector, for example using well-known gene cloning techniques such as PCR. Restriction sites may be introduced onto each end of the gene to facilitate cloning. For example, candidate genes may be amplified by PCR techniques using a set of primers. Both the amplified DNA and the cloning vector are cut with the same restriction enzymes, for example, NotI and PstI. The resulting fragments are gel-purified, ligated together, and transformed into E. coli. Plasmid DNA containing the vector with inserted gene may be isolated from E. coli cells selected for spectinomycin resistance, and the presence to the desired insert in pCGN8640 verified by digestion with the appropriate restriction enzymes. Undigested plasmid may then be transformed into Agrobacterium tumefaciens using techniques well known to those in the art, and transformed Agrobacterium cells containing the vector of interest selected based on spectinomycin resistance. These and other similar constructs useful for plant transformation may be readily prepared by one skilled in the art.

[0072] Transformation Methods and Transgenic Plants—Methods and compositions for transforming bacteria and other microorganisms are known in the art. See for example Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.).

[0073] Technology for introduction of DNA into cells is well known to those of skill in the art. Known methods for delivering a gene into cells include: (a) chemical methods (Graham and van der Eb (1973) Virology 54:536-539); (b) physical methods such as microinjection (Capecchi (1980) Cell 22:479-488), electroporation (Wong and Neumann (1982) Biochem. Biophys. Res. Commun. 107:584-587); Fromm et al. (1985) Proc. Natl. Acad. Sci. (U.S.A.) 82:5824-5828); U.S. Pat. No. 5,384,253); the gene gun (Johnston and Tang (1994) Methods Cell Biol. 43:353-365); (c) viral vectors (Clapp (1993) Clin. Perinatol. 20:155-168); Lu et al. (1993) J. Exp. Med. 178:2089-2096); Eglitis and Anderson (1988) Biotechniques 6:608-614); (d) receptor-mediated mechanisms (Curiel et al. (1992) Hum. Gen. Ther. 3:147-154), Wagner et al. (1992) Proc. Natl. Acad. Sci. (USA) 89:6099-6103); and (e) Agrobacterium tumefaciens-mediated transformation of plants (Fraley et al., Bio/Technology 3:629-635 (1985); and Rogers et al. (1987) Methods Enzymol. 153:253-277). In addition, DNA constructs and methods for stably transforming plant plastids have been described; see, for example U.S. Pat. No. 5,877,402, incorporated herein by reference.

[0074] After transformation, the transformed plant cells or tissues may be grown in an appropriate medium to promote cell proliferation and regeneration. In the case of protoplasts the cell wall will first be allowed to reform under appropriate osmotic conditions, and the resulting callus introduced into a nutrient regeneration medium to promote the formation of shoots and roots. For gene gun transformation of wheat and maize see U.S. Pat. Nos. 6,153,812 and 6,160,208, both of which are incorporated herein by reference. See also, Chistou (1996) Particle Bombardment for Genetic Engineering of Plants, Biotechnology Intelligence Unit, Academic Press, San Diego, Calif.), and in particular, pp. 63-69 (maize), and pp50-60 (rice).

[0075] The use of Agrobacterium-mediated plant integrating vectors to introduce DNA into plant cells for production of stably transformed whole plants is well known in the art. The region of DNA to be transferred into the host genome is defined by the tDNA border sequences in Agrobacterium-mediated plant integrating vectors and intervening DNA is usually inserted into the plant genome as described (Spielmann et al. (1986) Mol. Gen. Genet. 205:34). See also U.S. Pat. Nos. 5,416,011; 5,463,174; and 5,959,179 for Agrobacterium mediated transformation of soy; U.S. Pat. Nos. 5,591,616 and 5,731,179 for Agrobacterium mediated transformation of monocots such as maize; and U.S. Pat. No. 6,037,527 for Agrobacterium mediated transformation of cotton, all of which are incorporated herein by reference. Modern Agrobacterium transformation vectors are capable of replication in E. coli as well as Agrobacterium, allowing for convenient manipulations (Klee et al. (1985) In: Plant DNA Infectious Agents, Hohn and Schell (eds.), Springer-Verlag, New York, pp. 179-203).

[0076] Microprojectile bombardment techniques are also widely applicable, and may be used to transform virtually any plant species. Examples of species which have been transformed by microprojectile bombardment include monocot species such as maize (PCT Publication WO 95/06128), barley, wheat (U.S. Pat. No. 5,563,055), rice, oats, rye, sugarcane, and sorghum, and dicot species including tobacco, soybean (U.S. Pat. No. 5,322,783), sunflower, cotton, tomato, and legumes in general (U.S. Pat. No. 5,563,055).

[0077] Any of the constructs of the present invention may be introduced into a plant cell in a permanent or transient manner in combination with other genetic elements such as vectors, promoters enhancers etc. Further any of the constructs of the present invention may be introduced into a plant cell in a manner that allows for production in the plant cell of one or more polypeptides encoded by microbial polynucleotides in the construct.

[0078] It is also to be understood that two different transgenic plants can also be mated to produce offspring that contain two independently segregating added, exogenous genes. Selfing of appropriate progeny can produce plants that are homozygous for both added, exogenous genes that encode a polypeptide of interest. Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated, as is vegetative propagation.

[0079] Expression of microbial polynucleotides using constructs of the present invention and the concomitant production of polypeptides encoded by the polynucleotides is of interest for production of transgenic plants having improved properties, particularly, improved properties which result in crop plant yield improvement. Expression of polypeptides in plant cells may be evaluated by specifically identifying the protein products of the introduced genes or evaluating the phenotypic changes brought about by their expression.

[0080] Assays for the production and identification of specific proteins make use of various physical-chemical, structural, functional, or other properties of the proteins. Unique physical-chemical or structural properties allow the proteins to be separated and identified by electrophoretic procedures, such as native or denaturing gel electrophoresis or isoelectric focusing, or by chromatographic techniques such as ion exchange or gel exclusion chromatography. The unique structures of individual proteins offer opportunities for use of specific antibodies to detect their presence in formats such as an ELISA assay. Combinations of approaches may be employed with even greater specificity such as western blotting in which antibodies are used to locate individual gene products that have been separated by electrophoretic techniques. Additional techniques may be employed to absolutely confirm the identity of the product of interest such as evaluation by amino acid sequencing following purification. Although these are among the most commonly employed, other procedures may be additionally used.

[0081] Assay procedures may also be used to identify the expression of proteins by their functionality, particularly where the expressed protein is an enzyme capable of catalyzing chemical reactions involving specific substrates and products. These reactions may be measured, for example in plant extracts, by providing and quantifying the loss of substrates or the generation of products of the reactions by physical and/or chemical procedures.

[0082] In many cases, the expression of a gene product is determined by evaluating the phenotypic results of its expression. Such evaluations may be simply as visual observations, or may involve assays. Such assays may take many forms including but not limited to analyzing changes in the chemical composition, morphology, or physiological properties of the plant. Chemical composition may be altered by expression of genes encoding enzymes or storage proteins which change amino acid composition and may be detected by amino acid analysis, or by enzymes which change starch quantity which may be analyzed by near infrared reflectance spectrometry. Morphological changes may include greater stature or thicker stalks.

[0083] In addition to the above discussed procedures, practitioners are familiar with the standard resource materials which describe specific conditions and procedures for the construction, manipulation and isolation of macromolecules (e.g., DNA molecules, plasmids, etc.), generation of recombinant organisms and the screening and isolating of clones, (see for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press (1989); Mailga et al., Methods in Plant Molecular Biology, Cold Spring Harbor Press (1995; Birren et al., Genome Analysis: Analyzing DNA, 1, Cold Spring Harbor, N.Y. (1998)).

[0084] Having now generally described the invention, the same will be more readily understood through reference to the following example which is provided by way of illustration, and are not intended to be limiting of the present invention, unless specified.

[0085] Functions of polypeptides encoded by the microbial polynucleotide sequences described herein are determined using a hierarchical classification tool, termed FunCAT, for Functional Categories Annotation Tool. Most categories collected in FunCAT are classified by function, although other criteria are used, for example, cellular localization or temporal process. The assignment of a functional category to a query sequence is based on BLASTX sequence search, which compares two protein sequences. FunCAT assigns categories by iteratively scanning through all BLAST hits, starting with the most significant match, and reporting the first category assignment for each FunCAT source classification scheme. In the present invention, function of a query polypeptide is inferred from the function of a protein homolog where either (1) (hit_p<1e-30 or % identity>35%) AND query_coverage>50% AND hit_coverage>50%, or (2) hit_p<1e-8 AND query_coverage>70% AND hit_coverage>70%.

[0086] Functional assignments from five public classification schemes, GO_BP, GO_CC, GO_MF, KEGG and EC, and one internal Monsanto classification scheme, POI, are provided in Table 1. The column under the heading “cat_type” indicates the source of the classification. GO_BP=Gene Ontology Consortium−biological process; GO_CC=Gene Ontology Consortium−cellular component; GO_MF=Gene Ontology Consortium−molecular function; KEGG=KEGG functional hierarchy; EC=Enzyme Classification from ENZYME data bank release 25.0; POI=Pathways of Interest. The column under the heading “cat_desc” provides the name of the subcategory into which the query sequence was classified. The column under the heading “hit_desc” provides a description of the BLAST hit to the query sequences that led to the specific classification. The column under the heading “hit_p” provides the e-value for the BLAST hit.

[0087] Table 2 provides the SEQ ID NO (SEQ NUM), sequence designation (SEQ ID) of exemplary DNA sequence encoding the polypeptides described in Table 1. Table 2 also provides the SEQ ID NO (Prot Num) and sequence designation (Prot ID) of the proteins encoded by the nucleotide sequences.

[0088] Sequences useful for producing transgenic plants having improved biological properties are identified from their FunCAT annotations and are provided in Tables 3-21. a biological property of particular interest is plant yield. Plant yield may be improved by alteration of a variety of plant pathways, including those involving nitrogen, carbohydrate, or phosphorus utilization and/or uptake. Plant yield may also be improved by alteration of a plant's photosynthetic capacity or by improving a plant's ability to tolerate a variety of environmental stresses, including cold, heat, drought and osmotic stresses. Other biological properties of interest that may be improved using sequences of the present invention include pathogen or pest tolerance, herbicide tolerance, disease resistance, growth rate (for example by modification of cell cycle or expression of growth regulators), seed oil and/or protein yield and quality, rate and control of recombination, and lignin content.

[0089]

TABLE 3
Cold Tolerance
Table 3A SEQ ID NOs of Polypeptides useful for improving Cold Tolerance
4	5	6	11	19	26	31	36	39	52	56	81
88	90	91	101	109	117	118	121	122	128	145	146
147	151	153	154	156	178	191	195	204	230	238	247
257	272	274	275	295	310	321	325	332	338	342	350
356	378	379	390	393	395	396	400	413	418	436	437
443	449	456	459	466	477	479	491	508	510	515	516
525	526	531	541	543	544	548	549	555	570	571	576
585	587	599	609	623	631	636	646	648	664	665	672
681	682	684	696	725	744	752	756	757	761	782	787
788	815	822	835	852	854	859	871	874	883	885	890
895	897	903	904	909	937	964	975	976	980	983	985
987	993	995	997	1007	1024	1025	1035	1036	1041	1047	1055
1057	1070	1094	1105	1113	1123	1130	1132	1135	1145	1149	1173
1174	1183	1184	1185	1186	1200	1207	1214	1217	1220	1229	1241
1245	1250	1255	1292	1309	1310	1312	1313	1337	1341	1346	1347
1349	1358	1402	1403	1417	1434	1437	1459	1463	1464	1476	1477
1500	1507	1510	1552	1556	1564	1578	1597	1604	1615	1617	1624
1627	1632	1636	1664	1673	1685	1699	1702	1712	1740	1746	1748
1797	1834	1878	1883	1899	1903	1920	1928	1934	1952	1953	2007
2023	2042	2044	2051	2084	2097	2105	2107	2118	2124	2139	2146
2147	2148	2163	2174	2184	2186	2194	2198	2211	2212	2230	2233
2245	2254	2259	2263	2268	2270	2280	2286	2294	2302	2303	2304
2306	2311	2315	2321	2333	2377	2394	2413	2425	2430	2445	2450
2457	2485	2496	2497	2503	2517	2518	2546	2566	2567	2568	2599
2602	2612	2622	2625	2626	2632	2634	2642	2651	2687	2706	2708
2731	2739	2746	2750	2751	2752	2755	2768	2773	2788	2818	2824
2828	2835	2846	2847	2851	2853	2860	2864	2878	2882	2887	2894
2895	2896	2900	2905	2907	2916	2919	2929	2936	2945	2947	2959
2960	2968	2984	2987	2990	2991	3005	3012	3031	3035	3044	3047
3052	3057	3066	3069	3070	3100	3137	3138	3139	3156	3165	3180
3184	3191	3198	3200	3231	3234	3236	3240	3260	3271	3288	3291
3305	3334	3343	3357	3363	3366	3389	3395	3419	3441	3442	3469
3481	3482	3485	3488	3518	3522	3559	3561	3563	3570	3575	3588
3592	3597	3603	3658	3663	3683	3688	3700	3703	3705	3715	3722
3724	3733	3736	3741	3747	3751	3752	3757	3775	3780	3845	3853
3880	3888	3892	3931	3932	3938	3958	3966	3968	3976	3978	3987
3995	4006	4019	4021	4041	4075	4076	4078	4079	4093	4098	4100
4110	4117	4122	4142	4145	4154	4160	4178	4189	4195	4198	4202
4205	4234	4236	4272	4277	4280	4290	4291	4294	4295	4301	4302
4311	4312	4313	4316	4334	4362	4366	4375	4388	4391	4398	4416
4433	4438	4464	4487	4491	4496	4497	4505	4506	4507	4521	4548
4569	4580	4582	4590	4591	4599	4644	4657	4676	4686	4701	4706
4709	4721	4727	4729	4748	4762	4781	4783	4805	4806	4807	4811
4815	4818	4826	4829	4833	4837	4850	4866	4874	4885	4897	4898
4899	4911	4912	4920	4926	4960	4970	4979	4990	4991	4994	5002
5007	5012	5022	5035	5038	5045	5065	5089	5093	5097	5105	5116
5117	5118	5125	5154	5158	5180	5181	5183	5208	5212	5216	5226
5230	5233	5238	5239	5240	5241	5247	5248	5249	5250	5251	5252
5254	5255	5257	5268	5275	5278	5279	5298	5306	5320	5338	5339
5363	5378	5380	5394	5397	5399	5408	5423	5450	5468	5480	5485
5493	5496	5501	5516	5544	5547	5548	5550	5553	5567	5576	5588
5613	5614	5615	5616	5617	5618	5638	5640	5644	5650	5659	5660
5669	5686	5695	5698	5699	5700	5709	5713	5714	5717	5718	5723
5726	5727	5736	5769	5772	5777	5784	5785	5789	5795	5805	5812
5814	5827	5828	5855	5858	5866	5935	5936	5937	5950	5951	5955
5966	5968	5974	5993	6000	6001	6002	6007	6011	6014	6017	6018
6024	6028	6036	6042	6057	6076	6077	6086	6089	6095	6096	6115
6123	6135	6136	6144	6160	6163	6173	6174	6177	6188	6190	6193
6194	6219	6223	6231	6240	6241	6260	6287	6310	6312	6314	6315
6316	6317	6318	6322	6338	6343	6347	6348	6349	6350	6351	6352
6365	6366	6367	6374	6376	6378	6381	6388	6400	6421	6435	6442
6443	6449	6455	6456	6467	6470	6509	6510	6511	6515	6518	6520
6531	6532	6535	6536	6546	6549	6558	6587	6588	6589	6590	6591
6592	6596	6602	6617	6630	6637	6654	6668	6682	6702	6703	6706
6728	6730	6734	6749	6750	6756	6766	6768	6773	6776	6780	6796
6804	6819	6820	6828	6829	6836	6839	6845	6851	6854	6870	6879
6880	6922	6927	6940	6941	6977	6978	6979	6983	6991	7000	7001
7031	7035	7046	7047	7050	7051	7057	7058	7067	7068	7069	7072
7090	7116	7120	7129	7136	7143	7146	7153	7172	7191	7197	7224
7246	7250	7255	7256	7263	7264	7265	7266	7280	7326	7337	7339
7347	7348	7356	7402	7415	7432	7435	7445	7459	7465	7468	7480
7486	7488	7508	7521	7541	7543	7564	7565	7566	7570	7574	7577
7585	7588	7592	7597	7609	7625	7634	7643	7655	7656	7657	7669
7670	7678	7684	7718	7737	7743	7771	7772	7777	7782	7784	7794
7795	7805	7807	7808	7809	7810	7813	7815	7837	7840	7869	7873
7892	7896	7912	7913	7922	7939	7951	7966	7967	7973	7979	7993
8007	8011	8012	8015	8019	8022	8024	8039	8041	8047	8051	8057
8063	8074	8080	8083	8084	8093	8108	8112	8114	8133	8141	8159
8194	8195	8202	8203	8210	8219	8220	8224	8226	8241	8248	8249
8255	8256	8259	8260	8261	8262	8263	8265	8266	8270	8275	8276
8288	8290	8291	8297	8298	8304	8309	8319	8321	8323	8362	8374
8375	8406	8479	8484	8485	8488	8496	8502	8503	8513	8520	8530
8531	8545	8576	8578	8579	8581	8582	8589	8604	8606	8610	8611
8626	8631	8654	8676	8680	8685	8688	8692	8695	8699	8706	8707
8747	8750	8759	8760	8763	8767	8783	8806	8828	8843	8844	8852
8855	8859	8876	8880	8899	8901	8906	8913	8914	8922	8928	8939
8958	8977	8984	9005	9006	9017	9018	9019	9022	9023	9026	9029
9042	9061	9083	9088	9103	9105	9108	9123	9127	9134	9135	9136
9153	9156	9165	9167	9175	9186	9190	9193	9194	9195	9197	9198
9212	9227	9228	9236	9239	9240	9241	9243	9244	9245	9255	9257
9269	9270	9274	9276	9279	9280	9282	9303	9304	9320	9321	9329
9334	9352	9368	9369	9371	9372	9385	9386	9389	9390	9404	9409
9419	9420	9423	9424	9425	9435	9439	9440	9453	9460	9461	9462
9484	9490	9498	9507	9511	9520	9521	9524	9530	9539	9558	9575
9576	9586	9588	9589	9599	9612	9635	9645	9646	9650	9652	9669
9689	9690	9701	9713	9725	9732	9750	9751	9754	9758	9759	9774
9798	9814	9839	9856	9864	9866	9870	9874	9902	9928	9934	9960
9963	9976	9977	9981	9993	9995	9997	10026	10029	10038	10049	10061
10098	10099	10102	10104	10109	10114	10115	10121	10132	10135	10136	10143
10147	10151	10159	10163	10174	10181	10182	10185	10190	10204	10213	10223
10232	10262	10265	10266	10268	10285	10296	10304	10305	10311	10318	10319
10330	10336	10338	10339	10348	10357	10364	10365	10368	10370	10374	10381
10403	10404	10410	10411	10413	10417	10422	10431	10434	10438	10439	10445
10471	10478	10479	10487	10492	10514	10519	10521	10524	10530	10534	10543
10545	10572	10599	10600	10602	10603	10607	10615	10622	10629	10630	10646
10671	10672	10687	10690	10691	10705	10708	10736	10747	10750	10763	10765
10776	10782	10788	10789	10797	10798	10805	10814	10816	10850	10859	10872
10879	10880	10882	10898	10909	10910	10928	10968	10969	10970	10989	10990
10996	11005	11014	11028	11045	11050	11051	11058	11066	11073	11076	11079
11099	11102	11103	11105	11117	11119	11137	11160	11185	11188	11197	11216
11237	11245	11249	11251	11257	11262	11277	11285	11286	11297	11308	11309
11320	11321	11327	11329	11336	11338	11345	11364	11371	11374	11381	11387
11407	11413	11427	11432	11433	11439	11442	11443	11463	11465	11466	11468
11469	11475	11478	11490	11498	11506	11518	11535	11537	11538	11539	11540
11542	11550	11554	11555	11559	11565	11578	11594	11595	11597	11598	11614
11623	11629	11653	11661	11663	11667	11709	11719	11722	11747	11749	11766
11768	11769	11774	11783	11786	11795	11796	11800	11813	11830	11837	11838
11839	11840	11848	11850	11859	11875	11876	11881	11887	11897	11911	11932
11936	11950	11951	11977	11988	11997	12013	12021	12033	12034	12041	12052
12053	12058	12062	12071	12078	12079	12083	12086	12096	12099	12102	12104
12106	12108	12109	12110	12112	12124	12135	12138	12149	12159	12167	12176
12178	12186	12195	12200	12212	12213	12241	12275	12282	12302	12304	12312
12316	12324	12341	12363	12398	12400	12408	12418	12427	12438	12472	12482
12486	12492	12494	12496	12506	12509	12513	12517	12554	12566	12589	12593
12598	12605	12607	12610	12611	12626	12664	12673	12692	12704	12709	12723
12728	12730	12733	12736	12758	12771	12777	12835	12836	12889	12891	12903
12911	12926	12943	12949	12952	12968	12969	12984	12987	12989	13023	13037
13040	13064	13073	13090	13091	13100	13106	13108	13111	13126	13136	13141
13159	13166	13169	13171	13179	13192	13195	13212	13225	13234	13241	13247
13257	13280	13285	13295	13301	13313	13319	13329	13387	13401	13453	13454
13459	13473	13479	13480	13484	13499	13532	13534	13535	13536	13538	13539
13543	13554	13556	13557	13562	13568	13592	13596	13598	13603	13612	13633
13643	13648	13650	13653	13679	13688	13694	13717	13719	13720	13728	13749
13755	13758	13783	13786	13787	13797	13798	13806	13813	13817	13837	13845
13846	13851	13865	13866	13868	13873	13874	13880	13881	13887	13901	13903
13904	13906	13912	13920	13927	13932	13948	13953	13971	14030	14045	14048
14049	14053	14062	14067	14068	14113	14143	14145	14149	14152	14157	14175
14181	14195	14216	14232	14233	14234	14238	14242	14246	14247	14248	14255
14259	14266	14277	14283	14293	14304	14308	14309	14313	14322	14324	14331
14332	14333	14346	14348	14356	14364	14381	14383	14392	14400	14421	14431
14452	14453	14473	14474	14482	14484	14487	14488	14491	14501	14510	14517
14522	14523	14526	14528	14529	14534	14535	14540	14551	14565	14568	14575
14586	14588	14594	14598	14613	14615	14616	14649	14650	14652	14657	14662
14663	14664	14678	14700	14705	14707	14708	14711	14717	14723	14746	14752
14780	14795	14810	14820	14840	14847	14863	14864	14867	14869	14871	14873
14876	14882	14885	14894	14901	14920	14934	14938	14943	14962	14963	14964
14978	14983	14986	14991	15008	15016	15025	15029	15037	15047	15051	15058
15059	15060	15064	15079	15093	15103	15120	15123	15124	15125	15132	15140
15141	15142	15160	15183	15189	15190	15191	15193	15198	15202	15224	15229
15271	15289	15293	15310	15311	15312	15316	15317	15322	15334	15335	15336
15358	15360	15365	15374	15376	15377	15380	15388	15392	15398	15401	15406
15409	15415	15416	15422	15423	15428	15442	15454	15471	15482	15490	15492
15500	15518	15519	15526	15530	15534	15545	15553	15554	15561	15562	15575
15580	15582	15585	15587	15597	15601	15604	15609	15617	15624	15630	15634
15639	15642	15646	15647	15648	15649	15650	15651	15652	15654	15655	15656
15658	15659	15676	15677	15678	15682	15683	15688	15702	15703	15708	15725
15727	15732	15741	15744	15745	15748	15756	15760	15766	15769	15774	15777
15783	15784	15790	15791	15796	15799	15808	15811	15822	15841	15853	15862
15863	15871	15890	15891	15897	15901	15905	15917	15926	15927	15934	15935
15949	15951	15956	15958	15961	15963	15974	15979	15984	15992	15999	16005
16009	16014	16017	16021	16023	16024	16025	16026	16027	16028	16029	16030
16031	16033	16034	16035	16036	16037	16039	16041	16042	16045	16046	16047
16048	16049	16067	16068	16073	16074	16079	16092	16093	16094	16110	16111
16117	16126	16128	16129	16132	16139	16143	16150	16153	16157	16164	16166
16172	16173	16178	16181	16189	16190	16204	16233	16236	16238	16246	16263
16264	16269	16273	16284	16293	16294	16302	16303	16314	16315	16319	16324
16328	16336	16338	16342	16351	16352	16359	16363	16367	16370	16373	16376
16383	16386	16388	16389	16391	16407	16420	16421	16428	16429	16437	16445
16446	16457	16463	16478	16480	16481	16497	16519	16525	16550	16552	16558
16560	16561	16571	16576	16580	16590	16595	16598	16603	16610	16646	16662
16669	16679	16687	16690	16691	16693	16702	16703	16707	16709	16731	16735
16749	16756	16757	16776	16777	16783	16787	16799	16804	16805	16809	16825
16828	16844	16854	16864	16865	16866	16895	16901	16909	16915	16917	16920
16933	16935	16941	16946	16949	16959	16970	16980	16981	16987	16995	17009
17011	17015	17017	17024	17040	17058	17059	17065	17067	17068	17069	17088
17089	17093	17103	17125	17126	17155	17158	17182	17183	17184	17196	17198
17201	17204	17220	17226	17231	17234	17235	17237	17240	17273	17284	17319
17321	17330	17345	17348	17352	17359	17375	17388	17389	17399	17410	17427
17439	17440	17442	17447	17454	17466	17467	17476	17510	17515	17519	17522
17523	17551	17552	17554	17555	17556	17564	17565	17568	17570	17571	17572
17579	17587	17605	17614	17615	17619	17623	17626	17635	17636	17656	17658
17660	17661	17670	17671	17701	17722	17724	17725	17731	17738	17739	17742
17745	17749	17762	17786	17788	17790	17803	17807	17809	17822	17837	17840
17849	17863	17868	17870	17889	17898	17899	17901	17913	17914	17921	17926
17927	17934	17948	17958	17967	17971	17978	17981	17984	17985	17993	17995
18012	18015	18018	18025	18026	18030	18031	18034	18041	18043	18045	18049
18050	18051	18054	18055	18060	18063	18067	18069	18070	18071	18073	18074
18075	18076	18077	18078	18079	18080	18081	18082	18101	18113	18135	18143
18145	18149	18156	18176	18180	18192	18195	18204	18205	18213	18226	18227
18228	18241	18245	18255	18265	18267	18281	18308	18311	18330	18338	18345
18363	18367	18390	18397	18401	18402	18405	18410	18411	18417	18418	18424
18431	18432	18435	18442	18444	18447	18451	18473	18474	18475	18486	18487
18491	18500	18517	18522	18529	18537	18551	18569	18588	18595	18598	18608
18612	18617	18618	18625	18636	18640	18655	18660	18672	18673	18688	18692
18697	18699	18710	18718	18719	18731	18733	18740	18741	18751	18759	18761
18766	18768	18771	18804	18813	18824	18828	18838	18846	18858	18861	18862
18863	18869	18884	18904	18916	18925	18926	18930	18937	18941	18952	18953
18961	18963	18978	19005	19007	19008	19022	19023	19055	19059	19069	19073
19078	19118	19120	19137	19156	19158	19160	19169	19195	19198	19199	19200
19202	19203	19205	19216	19222	19233	19261	19264	19269	19276	19277	19280
19294	19300	19301	19317	19338	19351	19363	19364	19365	19366	19381	19383
19389	19390	19391	19394	19420	19433	19461	19464	19465	19472	19483	19486
19493	19502	19522	19549	19550	19553	19557	19572	19573	19575	19578	19580
19585	19593	19595	19599	19614	19616	19617	19620	19628	19644	19645	19656
19664	19666	19672	19684	19686	19698	19699	19703	19704	19708	19711	19714
19719	19754	19772	19791	19795	19799	19808	19831	19838	19844	19846	19847
19850	19852	19853	19854	19857	19860	19866	19915	19918	19921	19929	19930
19946	19956	19990	20007	20032	20035	20050	20055	20056	20068	20074	20086
20101	20103	20108	20110	20118	20119	20126	20133	20146	20155	20162	20192
20195	20207	20216	20220	20221	20229	20241	20253	20262	20278	20280	20297
20298	20301	20312	20317	20322	20358	20374	20375	20377	20378	20400	20404
20409	20410	20412	20417	20418	20419	20428	20437	20460	20461	20480	20515
20539	20543	20548	20580	20586	20603	20627	20637	20643	20646	20648	20655
20668	20709	20731	20736	20737	20739	20744	20745	20750	20757	20758	20765
20772	20784	20787	20793	20797	20801	20802	20805	20806	20811	20827	20828
20829	20835	20836	20840	20841	20848	20854	20868	20871	20878	20892	20894
20895	20910	20919	20921	20942	20943	20952	20963	20987	20988	20989	20991
20992	20993	21004	21008	21010	21032	21042	21045	21059	21062	21068	21073
21091	21103	21119	21124	21129	21147	21161	21167	21170	21185	21186	21190
21196	21200	21204	21209	21210	21243	21244	21256	21257	21260	21263	21267
21280	21281	21284	21285	21296	21313	21314	21323	21324	21328	21331	21340
21354	21364	21368	21371	21373	21379	21387	21396	21410	21425	21432	21441
21443	21446	21447	21460	21462	21464	21466	21481	21485	21503	21507	21513
21527	21533	21552	21557	21574	21585	21586	21588	21590	21607	21637	21639
21655	21664	21670	21677	21680	21682	21683	21689	21691	21692	21694	21701
21704	21711	21717	21722	21726	21729	21757	21758	21760	21763	21778	21781
21794	21795	21803	21809	21817	21822	21824	21825	21867	21872	21882	21883
21896	21900	21911	21929	21937	21941	21960	21973	21987	21988	21993	21994
21998	22006	22011	22013	22015	22034	22035	22036	22038	22066	22071	22078
22094	22101	22102	22107	22124	22126	22147	22165	22170	22186	22205	22206
22214	22220	22221	22232	22238	22253	22279	22291	22295	22332	22335	22346
22370	22378	22379	22386	22389	22391	22402	22406	22425	22428	22432	22436
22439	22446	22492	22497	22509	22538	22544	22545	22551	22552	22565	22582
22583	22594	22609	22617	22632	22642	22653	22675	22685	22712	22717	22737
22748	22759	22768	22782	22787	22799	22817	22823	22834	22839	22840	22843
22854	22864	22865	22870	22873	22895	22905	22928	22962	22980	23000	23013
23019	23052	23071	23073	23095	23110	23126	23127	23145	23146	23147	23176
23179	23181	23182	23183	23185	23202	23214	23217	23226	23227	23233	23241
23259	23263	23272	23290	23308	23312	23313	23318	23319	23321	23322	23326
23329	23350	23358	23363	23376	23392	23401	23420	23421	23426	23445	23459
23467	23474	23479	23480	23497	23498	23502	23505	23514	23516	23520	23524
23531	23535	23551	23578	23583	23598	23601	23604	23610	23612	23613	23619
23638	23640	23650	23651	23652	23653	23666	23667	23671	23673
Table 3B SEQ ID NOs of Polynucleotides useful for improving Cold Tolerance
23691	23692	23693	23698	23706	23713	23718	23723	23726	23739	23743	23768
23775	23777	23778	23788	23796	23804	23805	23808	23809	23815	23832	23833
23834	23838	23840	23841	23843	23865	23878	23882	23891	23917	23925	23934
23944	23959	23961	23962	23982	23997	24008	24012	24019	24025	24029	24037
24043	24065	24066	24077	24080	24082	24083	24087	24100	24105	24123	24124
24130	24136	24143	24146	24153	24164	24166	24178	24195	24197	24202	24203
24212	24213	24218	24228	24230	24231	24235	24236	24242	24257	24258	24263
24272	24274	24286	24296	24310	24318	24323	24333	24335	24351	24352	24359
24368	24369	24371	24383	24412	24431	24439	24443	24444	24448	24469	24474
24475	24502	24509	24522	24539	24541	24546	24558	24561	24570	24572	24577
24582	24584	24590	24591	24596	24624	24651	24662	24663	24667	24670	24672
24674	24680	24682	24684	24694	24711	24712	24722	24723	24728	24734	24742
24744	24757	24781	24792	24800	24810	24817	24819	24822	24832	24836	24860
24861	24870	24871	24872	24873	24887	24894	24901	24904	24907	24916	24928
24932	24937	24942	24979	24996	24997	24999	25000	25024	25028	25033	25034
25036	25045	25089	25090	25104	25121	25124	25146	25150	25151	25163	25164
25187	25194	25197	25239	25243	25251	25265	25284	25291	25302	25304	25311
25314	25319	25323	25351	25360	25372	25386	25389	25399	25427	25433	25435
25484	25521	25565	25570	25586	25590	25607	25615	25621	25639	25640	25694
25710	25729	25731	25738	25771	25784	25792	25794	25805	25811	25826	25833
25834	25835	25850	25861	25871	25873	25881	25885	25898	25899	25917	25920
25932	25941	25946	25950	25955	25957	25967	25973	25981	25989	25990	25991
25993	25998	26002	26008	26020	26064	26081	26100	26112	26117	26132	26137
26144	26172	26183	26184	26190	26204	26205	26233	26253	26254	26255	26286
26289	26299	26309	26312	26313	26319	26321	26329	26338	26374	26393	26395
26418	26426	26433	26437	26438	26439	26442	26455	26460	26475	26505	26511
26515	26522	26533	26534	26538	26540	26547	26551	26565	26569	26574	26581
26582	26583	26587	26592	26594	26603	26606	26616	26623	26632	26634	26646
26647	26655	26671	26674	26677	26678	26692	26699	26718	26722	26731	26734
26739	26744	26753	26756	26757	26787	26824	26825	26826	26843	26852	26867
26871	26878	26885	26887	26918	26921	26923	26927	26947	26958	26975	26978
26992	27021	27030	27044	27050	27053	27076	27082	27106	27128	27129	27156
27168	27169	27172	27175	27205	27209	27246	27248	27250	27257	27262	27275
27279	27284	27290	27345	27350	27370	27375	27387	27390	27392	27402	27409
27411	27420	27423	27428	27434	27438	27439	27444	27462	27467	27532	27540
27567	27575	27579	27618	27619	27625	27645	27653	27655	27663	27665	27674
27682	27693	27706	27708	27728	27762	27763	27765	27766	27780	27785	27787
27797	27804	27809	27829	27832	27841	27847	27865	27876	27882	27885	27889
27892	27921	27923	27959	27964	27967	27977	27978	27981	27982	27988	27989
27998	27999	28000	28003	28021	28049	28053	28062	28075	28078	28085	28103
28120	28125	28151	28174	28178	28183	28184	28192	28193	28194	28208	28235
28256	28267	28269	28277	28278	28286	28331	28344	28363	28373	28388	28393
28396	28408	28414	28416	28435	28449	28468	28470	28492	28493	28494	28498
28502	28505	28513	28516	28520	28524	28537	28553	28561	28572	28584	28585
28586	28598	28599	28607	28613	28647	28657	28666	28677	28678	28681	28689
28694	28699	28709	28722	28725	28732	28752	28776	28780	28784	28792	28803
28804	28805	28812	28841	28845	28867	28868	28870	28895	28899	28903	28913
28917	28920	28925	28926	28927	28928	28934	28935	28936	28937	28938	28939
28941	28942	28944	28955	28962	28965	28966	28985	28993	29007	29025	29026
29050	29065	29067	29081	29084	29086	29095	29110	29137	29155	29167	29172
29180	29183	29188	29203	29231	29234	29235	29237	29240	29254	29263	29275
29300	29301	29302	29303	29304	29305	29325	29327	29331	29337	29346	29347
29356	29373	29382	29385	29386	29387	29396	29400	29401	29404	29405	29410
29413	29414	29423	29456	29459	29464	29471	29472	29476	29482	29492	29499
29501	29514	29515	29542	29545	29553	29622	29623	29624	29637	29638	29642
29653	29655	29661	29680	29687	29688	29689	29694	29698	29701	29704	29705
29711	29715	29723	29729	29744	29763	29764	29773	29776	29782	29783	29802
29810	29822	29823	29831	29847	29850	29860	29861	29864	29875	29877	29880
29881	29906	29910	29918	29927	29928	29947	29974	29997	29999	30001	30002
30003	30004	30005	30009	30025	30030	30034	30035	30036	30037	30038	30039
30052	30053	30054	30061	30063	30065	30068	30075	30087	30108	30122	30129
30130	30136	30142	30143	30154	30157	30196	30197	30198	30202	30205	30207
30218	30219	30222	30223	30233	30236	30245	30274	30275	30276	30277	30278
30279	30283	30289	30304	30317	30324	30341	30355	30369	30389	30390	30393
30415	30417	30421	30436	30437	30443	30453	30455	30460	30463	30467	30483
30491	30506	30507	30515	30516	30523	30526	30532	30538	30541	30557	30566
30567	30609	30614	30627	30628	30664	30665	30666	30670	30678	30687	30688
30718	30722	30733	30734	30737	30738	30744	30745	30754	30755	30756	30759
30777	30803	30807	30816	30823	30830	30833	30840	30859	30878	30884	30911
30933	30937	30942	30943	30950	30951	30952	30953	30967	31013	31024	31026
31034	31035	31043	31089	31102	31119	31122	31132	31146	31152	31155	31167
31173	31175	31195	31208	31228	31230	31251	31252	31253	31257	31261	31264
31272	31275	31279	31284	31296	31312	31321	31330	31342	31343	31344	31356
31357	31365	31371	31405	31424	31430	31458	31459	31464	31469	31471	31481
31482	31492	31494	31495	31496	31497	31500	31502	31524	31527	31556	31560
31579	31583	31599	31600	31609	31626	31638	31653	31654	31660	31666	31680
31694	31698	31699	31702	31706	31709	31711	31726	31728	31734	31738	31744
31750	31761	31767	31770	31771	31780	31795	31799	31801	31820	31828	31846
31881	31882	31889	31890	31897	31906	31907	31911	31913	31928	31935	31936
31942	31943	31946	31947	31948	31949	31950	31952	31953	31957	31962	31963
31975	31977	31978	31984	31985	31991	31996	32006	32008	32010	32049	32061
32062	32093	32166	32171	32172	32175	32183	32189	32190	32200	32207	32217
32218	32232	32263	32265	32266	32268	32269	32276	32291	32293	32297	32298
32313	32318	32341	32363	32367	32372	32375	32379	32382	32386	32393	32394
32434	32437	32446	32447	32450	32454	32470	32493	32515	32530	32531	32539
32542	32546	32563	32567	32586	32588	32593	32600	32601	32609	32615	32626
32645	32664	32671	32692	32693	32704	32705	32706	32709	32710	32713	32716
32729	32748	32770	32775	32790	32792	32795	32810	32814	32821	32822	32823
32840	32843	32852	32854	32862	32873	32877	32880	32881	32882	32884	32885
32899	32914	32915	32923	32926	32927	32928	32930	32931	32932	32942	32944
32956	32957	32961	32963	32966	32967	32969	32990	32991	33007	33008	33016
33021	33039	33055	33056	33058	33059	33072	33073	33076	33077	33091	33096
33106	33107	33110	33111	33112	33122	33126	33127	33140	33147	33148	33149
33171	33177	33185	33194	33198	33207	33208	33211	33217	33226	33245	33262
33263	33273	33275	33276	33286	33299	33322	33332	33333	33337	33339	33356
33376	33377	33388	33400	33412	33419	33437	33438	33441	33445	33446	33461
33485	33501	33526	33543	33551	33553	33557	33561	33589	33615	33621	33647
33650	33663	33664	33668	33680	33682	33684	33713	33716	33725	33736	33748
33785	33786	33789	33791	33796	33801	33802	33808	33819	33822	33823	33830
33834	33838	33846	33850	33861	33868	33869	33872	33877	33891	33900	33910
33919	33949	33952	33953	33955	33972	33983	33991	33992	33998	34005	34006
34017	34023	34025	34026	34035	34044	34051	34052	34055	34057	34061	34068
34090	34091	34097	34098	34100	34104	34109	34118	34121	34125	34126	34132
34158	34165	34166	34174	34179	34201	34206	34208	34211	34217	34221	34230
34232	34259	34286	34287	34289	34290	34294	34302	34309	34316	34317	34333
34358	34359	34374	34377	34378	34392	34395	34423	34434	34437	34450	34452
34463	34469	34475	34476	34484	34485	34492	34501	34503	34537	34546	34559
34566	34567	34569	34585	34596	34597	34615	34655	34656	34657	34676	34677
34683	34692	34701	34715	34732	34737	34738	34745	34753	34760	34763	34766
34786	34789	34790	34792	34804	34806	34824	34847	34872	34875	34884	34903
34924	34932	34936	34938	34944	34949	34964	34972	34973	34984	34995	34996
35007	35008	35014	35016	35023	35025	35032	35051	35058	35061	35068	35074
35094	35100	35114	35119	35120	35126	35129	35130	35150	35152	35153	35155
35156	35162	35165	35177	35185	35193	35205	35222	35224	35225	35226	35227
35229	35237	35241	35242	35246	35252	35265	35281	35282	35284	35285	35301
35310	35316	35340	35348	35350	35354	35396	35406	35409	35434	35436	35453
35455	35456	35461	35470	35473	35482	35483	35487	35500	35517	35524	35525
35526	35527	35535	35537	35546	35562	35563	35568	35574	35584	35598	35619
35623	35637	35638	35664	35675	35684	35700	35708	35720	35721	35728	35739
35740	35745	35749	35758	35765	35766	35770	35773	35783	35786	35789	35791
35793	35795	35796	35797	35799	35811	35822	35825	35836	35846	35854	35863
35865	35873	35882	35887	35899	35900	35928	35962	35969	35989	35991	35999
36003	36011	36028	36050	36085	36087	36095	36105	36114	36125	36159	36169
36173	36179	36181	36183	36193	36196	36200	36204	36241	36253	36276	36280
36285	36292	36294	36297	36298	36313	36351	36360	36379	36391	36396	36410
36415	36417	36420	36423	36445	36458	36464	36522	36523	36576	36578	36590
36598	36613	36630	36636	36639	36655	36656	36671	36674	36676	36710	36724
36727	36751	36760	36777	36778	36787	36793	36795	36798	36813	36823	36828
36846	36853	36856	36858	36866	36879	36882	36899	36912	36921	36928	36934
36944	36967	36972	36982	36988	37000	37006	37016	37074	37088	37140	37141
37146	37160	37166	37167	37171	37186	37219	37221	37222	37223	37225	37226
37230	37241	37243	37244	37249	37255	37279	37283	37285	37290	37299	37320
37330	37335	37337	37340	37366	37375	37381	37404	37406	37407	37415	37436
37442	37445	37470	37473	37474	37484	37485	37493	37500	37504	37524	37532
37533	37538	37552	37553	37555	37560	37561	37567	37568	37574	37588	37590
37591	37593	37599	37607	37614	37619	37635	37640	37658	37717	37732	37735
37736	37740	37749	37754	37755	37800	37830	37832	37836	37839	37844	37862
37868	37882	37903	37919	37920	37921	37925	37929	37933	37934	37935	37942
37946	37953	37964	37970	37980	37991	37995	37996	38000	38009	38011	38018
38019	38020	38033	38035	38043	38051	38068	38070	38079	38087	38108	38118
38139	38140	38160	38161	38169	38171	38174	38175	38178	38188	38197	38204
38209	38210	38213	38215	38216	38221	38222	38227	38238	38252	38255	38262
38273	38275	38281	38285	38300	38302	38303	38336	38337	38339	38344	38349
38350	38351	38365	38387	38392	38394	38395	38398	38404	38410	38433	38439
38467	38482	38497	38507	38527	38534	38550	38551	38554	38556	38558	38560
38563	38569	38572	38581	38588	38607	38621	38625	38630	38649	38650	38651
38665	38670	38673	38678	38695	38703	38712	38716	38724	38734	38738	38745
38746	38747	38751	38766	38780	38790	38807	38810	38811	38812	38819	38827
38828	38829	38847	38870	38876	38877	38878	38880	38885	38889	38911	38916
38958	38976	38980	38997	38998	38999	39003	39004	39009	39021	39022	39023
39045	39047	39052	39061	39063	39064	39067	39075	39079	39085	39088	39093
39096	39102	39103	39109	39110	39115	39129	39141	39158	39169	39177	39179
39187	39205	39206	39213	39217	39221	39232	39240	39241	39248	39249	39262
39267	39269	39272	39274	39284	39288	39291	39296	39304	39311	39317	39321
39326	39329	39333	39334	39335	39336	39337	39338	39339	39341	39342	39343
39345	39346	39363	39364	39365	39369	39370	39375	39389	39390	39395	39412
39414	39419	39428	39431	39432	39435	39443	39447	39453	39456	39461	39464
39470	39471	39477	39478	39483	39486	39495	39498	39509	39528	39540	39549
39550	39558	39577	39578	39584	39588	39592	39604	39613	39614	39621	39622
39636	39638	39643	39645	39648	39650	39661	39666	39671	39679	39686	39692
39696	39701	39704	39708	39710	39711	39712	39713	39714	39715	39716	39717
39718	39720	39721	39722	39723	39724	39726	39728	39729	39732	39733	39734
39735	39736	39754	39755	39760	39761	39766	39779	39780	39781	39797	39798
39804	39813	39815	39816	39819	39826	39830	39837	39840	39844	39851	39853
39859	39860	39865	39868	39876	39877	39891	39920	39923	39925	39933	39950
39951	39956	39960	39971	39980	39981	39989	39990	40001	40002	40006	40011
40015	40023	40025	40029	40038	40039	40046	40050	40054	40057	40060	40063
40070	40073	40075	40076	40078	40094	40107	40108	40115	40116	40124	40132
40133	40144	40150	40165	40167	40168	40184	40206	40212	40237	40239	40245
40247	40248	40258	40263	40267	40277	40282	40285	40290	40297	40333	40349
40356	40366	40374	40377	40378	40380	40389	40390	40394	40396	40418	40422
40436	40443	40444	40463	40464	40470	40474	40486	40491	40492	40496	40512
40515	40531	40541	40551	40552	40553	40582	40588	40596	40602	40604	40607
40620	40622	40628	40633	40636	40646	40657	40667	40668	40674	40682	40696
40698	40702	40704	40711	40727	40745	40746	40752	40754	40755	40756	40775
40776	40780	40790	40812	40813	40842	40845	40869	40870	40871	40883	40885
40888	40891	40907	40913	40918	40921	40922	40924	40927	40960	40971	41006
41008	41017	41032	41035	41039	41046	41062	41075	41076	41086	41097	41114
41126	41127	41129	41134	41141	41153	41154	41163	41197	41202	41206	41209
41210	41238	41239	41241	41242	41243	41251	41252	41255	41257	41258	41259
41266	41274	41292	41301	41302	41306	41310	41313	41322	41323	41343	41345
41347	41348	41357	41358	41388	41409	41411	41412	41418	41425	41426	41429
41432	41436	41449	41473	41475	41477	41490	41494	41496	41509	41524	41527
41536	41550	41555	41557	41576	41585	41586	41588	41600	41601	41608	41613
41614	41621	41635	41645	41654	41658	41665	41668	41671	41672	41680	41682
41699	41702	41705	41712	41713	41717	41718	41721	41728	41730	41732	41736
41737	41738	41741	41742	41747	41750	41754	41756	41757	41758	41760	41761
41762	41763	41764	41765	41766	41767	41768	41769	41788	41800	41822	41830
41832	41836	41843	41863	41867	41879	41882	41891	41892	41900	41913	41914
41915	41928	41932	41942	41952	41954	41968	41995	41998	42017	42025	42032
42050	42054	42077	42084	42088	42089	42092	42097	42098	42104	42105	42111
42118	42119	42122	42129	42131	42134	42138	42160	42161	42162	42173	42174
42178	42187	42204	42209	42216	42224	42238	42256	42275	42282	42285	42295
42299	42304	42305	42312	42323	42327	42342	42347	42359	42360	42375	42379
42384	42386	42397	42405	42406	42418	42420	42427	42428	42438	42446	42448
42453	42455	42458	42491	42500	42511	42515	42525	42533	42545	42548	42549
42550	42556	42571	42591	42603	42612	42613	42617	42624	42628	42639	42640
42648	42650	42665	42692	42694	42695	42709	42710	42742	42746	42756	42760
42765	42805	42807	42824	42843	42845	42847	42856	42882	42885	42886	42887
42889	42890	42892	42903	42909	42920	42948	42951	42956	42963	42964	42967
42981	42987	42988	43004	43025	43038	43050	43051	43052	43053	43068	43070
43076	43077	43078	43081	43107	43120	43148	43151	43152	43159	43170	43173
43180	43189	43209	43236	43237	43240	43244	43259	43260	43262	43265	43267
43272	43280	43282	43286	43301	43303	43304	43307	43315	43331	43332	43343
43351	43353	43359	43371	43373	43385	43386	43390	43391	43395	43398	43401
43406	43441	43459	43478	43482	43486	43495	43518	43525	43531	43533	43534
43537	43539	43540	43541	43544	43547	43553	43602	43605	43608	43616	43617
43633	43643	43677	43694	43719	43722	43737	43742	43743	43755	43761	43773
43788	43790	43795	43797	43805	43806	43813	43820	43833	43842	43849	43879
43882	43894	43903	43907	43908	43916	43928	43940	43949	43965	43967	43984
43985	43988	43999	44004	44009	44045	44061	44062	44064	44065	44087	44091
44096	44097	44099	44104	44105	44106	44115	44124	44147	44148	44167	44202
44226	44230	44235	44267	44273	44290	44314	44324	44330	44333	44335	44342
44355	44396	44418	44423	44424	44426	44431	44432	44437	44444	44445	44452
44459	44471	44474	44480	44484	44488	44489	44492	44493	44498	44514	44515
44516	44522	44523	44527	44528	44535	44541	44555	44558	44565	44579	44581
44582	44597	44606	44608	44629	44630	44639	44650	44674	44675	44676	44678
44679	44680	44691	44695	44697	44719	44729	44732	44746	44749	44755	44760
44778	44790	44806	44811	44816	44834	44848	44854	44857	44872	44873	44877
44883	44887	44891	44896	44897	44930	44931	44943	44944	44947	44950	44954
44967	44968	44971	44972	44983	45000	45001	45010	45011	45015	45018	45027
45041	45051	45055	45058	45060	45066	45074	45083	45097	45112	45119	45128
45130	45133	45134	45147	45149	45151	45153	45168	45172	45190	45194	45200
45214	45220	45239	45244	45261	45272	45273	45275	45277	45294	45324	45326
45342	45351	45357	45364	45367	45369	45370	45376	45378	45379	45381	45388
45391	45398	45404	45409	45413	45416	45444	45445	45447	45450	45465	45468
45481	45482	45490	45496	45504	45509	45511	45512	45554	45559	45569	45570
45583	45587	45598	45616	45624	45628	45647	45660	45674	45675	45680	45681
45685	45693	45698	45700	45702	45721	45722	45723	45725	45753	45758	45765
45781	45788	45789	45794	45811	45813	45834	45852	45857	45873	45892	45893
45901	45907	45908	45919	45925	45940	45966	45978	45982	46019	46022	46033
46057	46065	46066	46073	46076	46078	46089	46093	46112	46115	46119	46123
46126	46133	46179	46184	46196	46225	46231	46232	46238	46239	46252	46269
46270	46281	46296	46304	46319	46329	46340	46362	46372	46399	46404	46424
46435	46446	46455	46469	46474	46486	46504	46510	46521	46526	46527	46530
46541	46551	46552	46557	46560	46582	46592	46615	46649	46667	46687	46700
46706	46739	46758	46760	46782	46797	46813	46814	46832	46833	46834	46863
46866	46868	46869	46870	46872	46889	46901	46904	46913	46914	46920	46928
46946	46950	46959	46977	46995	46999	47000	47005	47006	47008	47009	47013
47016	47037	47045	47050	47063	47079	47088	47107	47108	47113	47132	47146
47154	47161	47166	47167	47184	47185	47189	47192	47201	47203	47207	47211
47218	47222	47238	47265	47270	47285	47288	47291	47297	47299	47300	47306
47325	47327	47337	47338	47339	47340	47353	47354	47358	47360

[0090]

TABLE 4
Disease Control
Table 4A SEQ ID NOs of Polypeptides useful for Improving Disease Control
415	492	507	653	721	833	1355	1356	1472	1539	1545	1684
1802	1878	1928	1932	1957	2023	2335	2337	2880	2924	3089	3135
3308	3399	3492	3566	3785	3812	3831	3951	4035	4049	4051	4114
4125	4167	4288	4408	4456	4659	4680	4711	4743	4756	4824	4838
4949	5146	5302	5381	5421	5520	5521	5522	5523	5524	5525	5664
5897	5984	6023	6195	6215	6248	6303	6353	6391	6450	6507	6508
6581	6582	6583	6743	6901	7044	7164	7216	7418	7439	7471	7503
7515	7583	7707	7816	7890	7983	7993	8158	8165	8236	8244	8470
8547	8599	8641	8700	8722	8812	8961	9115	9117	9120	9281	9309
9398	9399	10218	10228	10461	10562	10717	10803	10892	10919	11040	11058
11065	11114	11177	11298	11345	11395	11462	11500	11527	11556	11684	11765
11807	11860	11865	11889	11934	12024	12262	12263	12329	12339	12435	12478
12511	12631	12670	12727	12804	12818	12910	12950	13012	13016	13060	13100
13135	13147	13220	13288	13372	13482	13557	13631	13656	13690	13691	13805
13850	13927	14042	14080	14111	14179	14207	14280	14347	14420	14475	14511
14593	14671	14728	14787	14789	14922	14948	14953	14961	15106	15152	15208
15360	15414	15727	15782	15948	16111	16165	16394	16399	16459	16514	16538
16543	16644	16742	16746	16840	16849	16889	16891	17022	17136	17162	17274
17479	17563	17612	17614	17735	17952	18118	18204	18212	18262	18393	18493
18503	18584	18585	18613	18984	19136	19211	19289	19361	19400	19484	19507
19514	19529	19684	19726	19739	19750	19803	19830	19890	19963	19972	20183
20187	20189	20271	20427	20462	20499	20552	20590	20624	20721	20851	20915
21113	21172	21317	21318	21322	22719	22735	22884	22908	22951	23041	23167
23211	23365	23452	23465	23680	23682	23685
Table 4B SEQ ID NOs of Polynucleotides useful for Improving Disease Control
24102	24179	24194	24340	24408	24520	25042	25043	25159	25226	25232	25371
25489	25565	25615	25619	25644	25710	26022	26024	26567	26611	26776	26822
26995	27086	27179	27253	27472	27499	27518	27638	27722	27736	27738	27801
27812	27854	27975	28095	28143	28346	28367	28398	28430	28443	28511	28525
28636	28833	28989	29068	29108	29207	29208	29209	29210	29211	29212	29351
29584	29671	29710	29882	29902	29935	29990	30040	30078	30137	30194	30195
30268	30269	30270	30430	30588	30731	30851	30903	31105	31126	31158	31190
31202	31270	31394	31503	31577	31670	31680	31845	31852	31923	31931	32157
32234	32286	32328	32387	32409	32499	32648	32802	32804	32807	32968	32996
33085	33086	33905	33915	34148	34249	34404	34490	34579	34606	34727	34745
34752	34801	34864	34985	35032	35082	35149	35187	35214	35243	35371	35452
35494	35547	35552	35576	35621	35711	35949	35950	36016	36026	36122	36165
36198	36318	36357	36414	36491	36505	36597	36637	36699	36703	36747	36787
36822	36834	36907	36975	37059	37169	37244	37318	37343	37377	37378	37492
37537	37614	37729	37767	37798	37866	37894	37967	38034	38107	38162	38198
38280	38358	38415	38474	38476	38609	38635	38640	38648	38793	38839	38895
39047	39101	39414	39469	39635	39798	39852	40081	40086	40146	40201	40225
40230	40331	40429	40433	40527	40536	40576	40578	40709	40823	40849	40961
41166	41250	41299	41301	41422	41639	41805	41891	41899	41949	42080	42180
42190	42271	42272	42300	42671	42823	42898	42976	43048	43087	43171	43194
43201	43216	43371	43413	43426	43437	43490	43517	43577	43650	43659	43870
43874	43876	43958	44114	44149	44186	44239	44277	44311	44408	44538	44602
44800	44859	45004	45005	45009	46406	46422	46571	46595	46638	46728	46854
46898	47052	47139	47152	47367	47369	47372

[0091]

TABLE 5
Drought Tolerance
Table 5A SEQ ID NOs of Polypeptides useful for Improving Drought Tolerance
390	752	874	1463	1624	1883	1920	2245	2268	2311	2546	2751
2968	3030	3191	3411	3588	3853	4019	4375	4438	4644	4706	4960
5268	5378	7129	7197	7402	7465	7718	7973	8022	8321	8654	9976
10422	10519	10572	10616	11099	11374	11737	11837	12302	12438	12607	12704
12968	13045	13234	13603	14304	14427	14780	14793	14934	14959	15271	15597
15604	15974	15979	16336	16338	16731	17184	17803	18255	18766	19269	19383
19844	19866	20146	20637	20793	20943	21062	21190	21533	21717	22074	22238
22749	22787	23212	23310	23354	23445
Table 5B SEQ ID NOs of Polynucleotides useful for Improving Drought Tolerance
24077	24439	24561	25150	25311	25570	25607	25932	25955	25998	26233	26438
26655	26717	26878	27098	27275	27540	27706	28062	28125	28331	28393	28647
28955	29065	30816	30884	31089	31152	31405	31660	31709	32008	32341	33663
34109	34206	34259	34303	34786	35061	35424	35524	35989	36125	36294	36391
36655	36732	36921	37290	37991	38114	38467	38480	38621	38646	38958	39284
39291	39661	39666	40023	40025	40418	40871	41490	41942	42453	42956	43070
43531	43553	43833	44324	44480	44630	44749	44877	45220	45404	45761	45925
46436	46474	46899	46997	47041	47132

[0092]

TABLE 6
Plant Growth/Cell Cycle
Table 6A SEQ ID NOs of Polypeptides useful for improving Plant Growth - Cell Cycle
38	52	83	91	152	160	167	184	212	229	258	284
295	300	355	363	444	553	614	617	623	627	628	658
670	723	746	844	859	865	866	870	872	921	929	939
942	944	959	960	963	974	984	985	1005	1006	1031	1041
1056	1062	1083	1098	1103	1116	1117	1124	1125	1168	1180	1213
1217	1230	1242	1246	1247	1249	1251	1272	1273	1279	1286	1287
1293	1305	1306	1312	1318	1322	1350	1351	1352	1353	1354	1355
1356	1357	1358	1359	1360	1361	1362	1363	1364	1365	1366	1367
1368	1369	1370	1371	1372	1373	1374	1375	1376	1377	1378	1379
1380	1381	1382	1383	1384	1385	1386	1387	1388	1389	1390	1398
1399	1400	1407	1422	1424	1438	1448	1452	1455	1467	1475	1487
1493	1503	1524	1533	1535	1540	1542	1551	1553	1561	1563	1568
1569	1570	1576	1581	1585	1586	1591	1619	1620	1621	1625	1627
1628	1630	1650	1653	1655	1678	1715	1735	1744	1761	1768	1770
1776	1790	1798	1800	1842	1852	1855	1870	1875	1891	1894	1901
1908	1919	1938	1942	1965	1984	1990	2001	2009	2012	2030	2033
2036	2037	2040	2061	2070	2072	2073	2076	2088	2099	2101	2114
2116	2133	2135	2136	2138	2150	2157	2161	2179	2184	2187	2188
2210	2213	2217	2219	2224	2234	2239	2240	2248	2257	2258	2276
2290	2294	2302	2309	2317	2318	2323	2324	2326	2335	2337	2342
2344	2347	2348	2353	2362	2370	2371	2375	2376	2382	2390	2391
2395	2400	2401	2405	2434	2447	2452	2454	2465	2468	2469	2472
2473	2479	2487	2491	2498	2502	2505	2525	2544	2553	2556	2563
2565	2577	2581	2582	2584	2590	2591	2593	2594	2691	2693	2823
2840	2846	2853	2904	2906	2924	2940	2987	3033	3035	3070	3075
3084	3092	3093	3101	3112	3118	3134	3142	3151	3162	3174	3195
3209	3210	3227	3230	3242	3247	3255	3256	3268	3272	3275	3279
3293	3294	3318	3319	3329	3340	3345	3346	3347	3349	3368	3370
3371	3374	3375	3377	3386	3388	3402	3405	3406	3408	3417	3433
3434	3438	3449	3456	3464	3467	3477	3487	3491	3494	3503	3519
3526	3539	3553	3557	3564	3565	3569	3572	3574	3585	3587	3606
3607	3613	3626	3648	3649	3650	3653	3676	3691	3700	3706	3707
3713	3716	3718	3719	3729	3732	3734	3738	3753	3767	3768	3790
3791	3801	3802	3807	3822	3829	3841	3847	3870	3883	3892	3903
3904	3909	3922	3926	3933	3935	3938	3941	3942	3944	3950	3959
3961	3963	3965	3975	3989	4007	4025	4026	4036	4039	4053	4055
4058	4062	4067	4070	4073	4087	4089	4090	4091	4102	4120	4132
4148	4151	4155	4156	4170	4174	4193	4195	4197	4199	4208	4222
4239	4261	4314	4347	4363	4371	4385	4389	4408	4416	4435	4509
4511	4525	4548	4634	4636	4658	4666	4692	4703	4735	4736	4773
4813	4850	4859	4920	4934	4947	4967	4977	4980	4988	4992	4995
5002	5014	5018	5030	5053	5094	5101	5102	5109	5110	5113	5128
5129	5137	5138	5141	5156	5159	5162	5163	5172	5173	5174	5175
5186	5190	5191	5192	5207	5227	5234	5235	5236	5237	5271	5296
5297	5305	5307	5308	5341	5342	5357	5366	5368	5372	5388	5400
5413	5414	5420	5447	5455	5459	5460	5478	5482	5505	5506	5514
5517	5519	5526	5552	5558	5566	5571	5601	5607	5609	5620	5639
5643	5653	5663	5671	5672	5680	5690	5692	5696	5697	5705	5719
5721	5733	5739	5743	5746	5769	5781	5791	5797	5822	5838	5839
5840	5848	5849	5853	5854	5855	5874	5875	5877	5881	5887	5890
5894	5895	5901	5902	5904	5920	5929	5930	5940	5949	5955	5964
5972	5973	5975	5977	5979	5990	6020	6026	6027	6063	6064	6067
6074	6092	6106	6117	6118	6119	6143	6150	6156	6157	6161	6187
6188	6189	6194	6200	6201	6209	6210	6237	6246	6247	6248	6270
6289	6298	6304	6328	6330	6343	6346	6362	6364	6376	6390	6395
6397	6432	6440	6449	6453	6460	6479	6480	6481	6501	6516	6527
6564	6573	6586	6597	6604	6612	6621	6625	6631	6638	6639	6665
6676	6724	6727	6729	6751	6770	6774	6821	6832	6834	6840	6841
6844	6888	6922	6924	6925	6926	6928	6929	6942	6943	6963	6964
6973	6974	6975	6995	6998	7016	7017	7027	7029	7030	7070	7102
7117	7125	7140	7144	7164	7172	7194	7267	7269	7284	7322	7389
7392	7394	7416	7417	7461	7495	7533	7572	7609	7618	7678	7692
7705	7725	7751	7765	7797	7800	7824	7852	7887	7931	7951	7964
8029	8067	8074	8115	8119	8131	8199	8215	8228	8233	8259	8267
8278	8286	8458	8467	8474	8493	8650	8678	8685	8695	8702	8731
8773	8794	8804	8832	8847	8849	8851	8878	8880	8933	8961	8973
8974	9007	9015	9075	9076	9088	9103	9114	9141	9250	9265	9271
9282	9287	9297	9301	9376	9430	9476	9505	9524	9536	9541	9625
9646	9731	9770	9790	9839	9853	9876	9899	9967	10053	10100	10109
10111	10140	10154	10158	10249	10254	10259	10287	10321	10375	10395	10404
10414	10454	10472	10545	10562	10583	10621	10644	10646	10683	10684	10855
10878	10887	10890	10892	10901	10914	10917	10933	10954	10959	10987	11000
11003	11005	11023	11031	11044	11060	11071	11104	11105	11107	11108	11119
11148	11160	11164	11228	11236	11275	11277	11279	11291	11295	11302	11312
11330	11333	11335	11354	11379	11384	11411	11453	11462	11487	11505	11539
11565	11606	11623	11660	11663	11689	11700	11798	11841	11849	11860	11869
11887	11907	11951	11980	11988	12017	12040	12114	12128	12150	12192	12215
12249	12336	12365	12387	12411	12415	12424	12429	12442	12443	12464	12474
12512	12534	12545	12547	12555	12557	12560	12561	12564	12567	12571	12578
12581	12582	12584	12588	12591	12604	12609	12616	12646	12649	12651	12654
12660	12677	12685	12687	12705	12719	12724	12732	12740	12745	12752	12754
12755	12756	12775	12779	12785	12831	12839	12856	12862	12867	12885	12886
12888	12898	12915	12917	12931	12940	12945	12948	12951	12954	12955	12961
12965	12966	12972	12977	12992	12998	13005	13007	13066	13077	13082	13083
13084	13088	13093	13103	13114	13117	13118	13132	13133	13141	13159	13162
13178	13208	13223	13227	13231	13238	13243	13248	13270	13273	13290	13300
13302	13315	13342	13363	13372	13374	13376	13383	13398	13399	13402	13404
13407	13412	13413	13414	13415	13445	13464	13482	13487	13489	13490	13495
13500	13504	13513	13538	13539	13543	13570	13572	13580	13581	13583	13590
13593	13606	13607	13609	13635	13640	13653	13683	13708	13726	13730	13737
13773	13776	13808	13840	13857	13919	13947	13955	13971	13985	14059	14084
14156	14254	14280	14283	14293	14309	14313	14315	14318	14387	14390	14428
14473	14474	14482	14532	14540	14552	14606	14661	14702	14728	14731	14742
14867	15007	15054	15079	15093	15103	15121	15138	15184	15208	15211	15477
15503	15512	15548	15559	15601	15635	15808	15847	15875	15884	15920	15921
15932	15970	16010	16022	16040	16044	16189	16226	16250	16257	16287	16289
16299	16364	16382	16431	16504	16508	16524	16543	16545	16582	16638	16647
16692	16733	16787	16837	16940	16984	17007	17048	17073	17091	17107	17108
17155	17189	17192	17226	17231	17271	17284	17348	17398	17403	17405	17428
17476	17479	17482	17499	17503	17524	17537	17645	17676	17704	17796	17809
17845	17971	18005	18013	18057	18060	18109	18110	18116	18137	18148	18176
18184	18190	18192	18195	18198	18211	18215	18216	18218	18225	18229	18244
18253	18256	18257	18258	18268	18284	18288	18350	18393	18424	18430	18450
18481	18537	18538	18542	18543	18544	18559	18566	18569	18579	18589	18594
18602	18612	18638	18645	18664	18666	18670	18676	18677	18685	18686	18689
18702	18755	18771	18775	18818	18823	18897	18932	18939	18994	18997	19033
19034	19044	19074	19126	19139	19146	19152	19166	19176	19218	19262	19310
19319	19330	19381	19415	19417	19433	19495	19522	19527	19548	19565	19576
19581	19616	19640	19655	19656	19662	19690	19831	19859	19935	19939	19947
19958	19972	19984	19993	20050	20102	20109	20141	20143	20185	20203	20256
20281	20313	20314	20316	20318	20338	20339	20345	20352	20353	20359	20371
20377	20383	20387	20450	20482	20486	20527	20547	20553	20554	20557	20581
20643	20706	20707	20721	20746	20787	20817	20826	20851	20910	20920	20946
21075	21077	21123	21130	21221	21247	21269	21304	21322	21348	21352	21359
21388	21389	21407	21409	21414	21436	21437	21451	21452	21457	21469	21482
21490	21521	21522	21524	21538	21575	21580	21586	21594	21595	21606	21609
21621	21634	21635	21636	21663	21665	21666	21668	21695	21779	21813	21816
21817	21868	21874	21888	21890	21899	21902	21904	21907	21910	21923	21924
21927	21931	21940	21949	21955	21957	21964	21967	21970	21976	21977	21978
21981	21985	21989	21995	21997	22005	22025	22030	22042	22059	22068	22069
22073	22080	22084	22087	22092	22103	22104	22105	22108	22133	22142	22149
22150	22151	22153	22171	22173	22174	22179	22189	22199	22211	22221	22228
22235	22246	22247	22262	22272	22276	22285	22288	22292	22304	22311	22312
22317	22318	22319	22327	22338	22343	22349	22354	22356	22360	22364	22387
22421	22422	22423	22426	22427	22429	22430	22434	22435	22437	22439	22449
22453	22456	22457	22462	22488	22500	22502	22505	22511	22513	22520	22526
22530	22531	22548	22556	22559	22561	22562	22564	22578	22595	22604	22616
22618	22624	22625	22627	22641	22656	22669	22673	22674	22686	22695	22702
22703	22704	22712	22718	22725	22734	22741	22742	22745	22747	22771	22778
22784	22785	22802	22806	22811	22818	22820	22829	22832	22838	22841	22842
22860	22866	22869	22884	22892	22895	22929	22939	22942	22950	22960	22961
22974	23038	23039	23095	23098	23128	23134	23183	23185	23230	23240	23247
23249	23261	23276	23284	23350	23423	23435	23458	23473	23535	23543	23564
23606	23658	23680	23685
Table 6B SEQ ID NOs of Polynucleotides useful for improving Plant Growth - Cell Cycle
23725	23739	23770	23778	23839	23847	23854	23871	23899	23916	23945	23971
23982	23987	24042	24050	24131	24240	24301	24304	24310	24314	24315	24345
24357	24410	24433	24531	24546	24552	24553	24557	24559	24608	24616	24626
24629	24631	24646	24647	24650	24661	24671	24672	24692	24693	24718	24728
24743	24749	24770	24785	24790	24803	24804	24811	24812	24855	24867	24900
24904	24917	24929	24933	24934	24936	24938	24959	24960	24966	24973	24974
24980	24992	24993	24999	25005	25009	25037	25038	25039	25040	25041	25042
25043	25044	25045	25046	25047	25048	25049	25050	25051	25052	25053	25054
25055	25056	25057	25058	25059	25060	25061	25062	25063	25064	25065	25066
25067	25068	25069	25070	25071	25072	25073	25074	25075	25076	25077	25085
25086	25087	25094	25109	25111	25125	25135	25139	25142	25154	25162	25174
25180	25190	25211	25220	25222	25227	25229	25238	25240	25248	25250	25255
25256	25257	25263	25268	25272	25273	25278	25306	25307	25308	25312	25314
25315	25317	25337	25340	25342	25365	25402	25422	25431	25448	25455	25457
25463	25477	25485	25487	25529	25539	25542	25557	25562	25578	25581	25588
25595	25606	25625	25629	25652	25671	25677	25688	25696	25699	25717	25720
25723	25724	25727	25748	25757	25759	25760	25763	25775	25786	25788	25801
25803	25820	25822	25823	25825	25837	25844	25848	25866	25871	25874	25875
25897	25900	25904	25906	25911	25921	25926	25927	25935	25944	25945	25963
25977	25981	25989	25996	26004	26005	26010	26011	26013	26022	26024	26029
26031	26034	26035	26040	26049	26057	26058	26062	26063	26069	26077	26078
26082	26087	26088	26092	26121	26134	26139	26141	26152	26155	26156	26159
26160	26166	26174	26178	26185	26189	26192	26212	26231	26240	26243	26250
26252	26264	26268	26269	26271	26277	26278	26280	26281	26378	26380	26510
26527	26533	26540	26591	26593	26611	26627	26674	26720	26722	26757	26762
26771	26779	26780	26788	26799	26805	26821	26829	26838	26849	26861	26882
26896	26897	26914	26917	26929	26934	26942	26943	26955	26959	26962	26966
26980	26981	27005	27006	27016	27027	27032	27033	27034	27036	27055	27057
27058	27061	27062	27064	27073	27075	27089	27092	27093	27095	27104	27120
27121	27125	27136	27143	27151	27154	27164	27174	27178	27181	27190	27206
27213	27226	27240	27244	27251	27252	27256	27259	27261	27272	27274	27293
27294	27300	27313	27335	27336	27337	27340	27363	27378	27387	27393	27394
27400	27403	27405	27406	27416	27419	27421	27425	27440	27454	27455	27477
27478	27488	27489	27494	27509	27516	27528	27534	27557	27570	27579	27590
27591	27596	27609	27613	27620	27622	27625	27628	27629	27631	27637	27646
27648	27650	27652	27662	27676	27694	27712	27713	27723	27726	27740	27742
27745	27749	27754	27757	27760	27774	27776	27777	27778	27789	27807	27819
27835	27838	27842	27843	27857	27861	27880	27882	27884	27886	27895	27909
27926	27948	28001	28034	28050	28058	28072	28076	28095	28103	28122	28196
28198	28212	28235	28321	28323	28345	28353	28379	28390	28422	28423	28460
28500	28537	28546	28607	28621	28634	28654	28664	28667	28675	28679	28682
28689	28701	28705	28717	28740	28781	28788	28789	28796	28797	28800	28815
28816	28824	28825	28828	28843	28846	28849	28850	28859	28860	28861	28862
28873	28877	28878	28879	28894	28914	28921	28922	28923	28924	28958	28983
28984	28992	28994	28995	29028	29029	29044	29053	29055	29059	29075	29087
29100	29101	29107	29134	29142	29146	29147	29165	29169	29192	29193	29201
29204	29206	29213	29239	29245	29253	29258	29288	29294	29296	29307	29326
29330	29340	29350	29358	29359	29367	29377	29379	29383	29384	29392	29406
29408	29420	29426	29430	29433	29456	29468	29478	29484	29509	29525	29526
29527	29535	29536	29540	29541	29542	29561	29562	29564	29568	29574	29577
29581	29582	29588	29589	29591	29607	29616	29617	29627	29636	29642	29651
29659	29660	29662	29664	29666	29677	29707	29713	29714	29750	29751	29754
29761	29779	29793	29804	29805	29806	29830	29837	29843	29844	29848	29874
29875	29876	29881	29887	29888	29896	29897	29924	29933	29934	29935	29957
29976	29985	29991	30015	30017	30030	30033	30049	30051	30063	30077	30082
30084	30119	30127	30136	30140	30147	30166	30167	30168	30188	30203	30214
30251	30260	30273	30284	30291	30299	30308	30312	30318	30325	30326	30352
30363	30411	30414	30416	30438	30457	30461	30508	30519	30521	30527	30528
30531	30575	30609	30611	30612	30613	30615	30616	30629	30630	30650	30651
30660	30661	30662	30682	30685	30703	30704	30714	30716	30717	30757	30789
30804	30812	30827	30831	30851	30859	30881	30954	30956	30971	31009	31076
31079	31081	31103	31104	31148	31182	31220	31259	31296	31305	31365	31379
31392	31412	31438	31452	31484	31487	31511	31539	31574	31618	31638	31651
31716	31754	31761	31802	31806	31818	31886	31902	31915	31920	31946	31954
31965	31973	32145	32154	32161	32180	32337	32365	32372	32382	32389	32418
32460	32481	32491	32519	32534	32536	32538	32565	32567	32620	32648	32660
32661	32694	32702	32762	32763	32775	32790	32801	32828	32937	32952	32958
32969	32974	32984	32988	33063	33117	33163	33192	33211	33223	33228	33312
33333	33418	33457	33477	33526	33540	33563	33586	33654	33740	33787	33796
33798	33827	33841	33845	33936	33941	33946	33974	34008	34062	34082	34091
34101	34141	34159	34232	34249	34270	34308	34331	34333	34370	34371	34542
34565	34574	34577	34579	34588	34601	34604	34620	34641	34646	34674	34687
34690	34692	34710	34718	34731	34747	34758	34791	34792	34794	34795	34806
34835	34847	34851	34915	34923	34962	34964	34966	34978	34982	34989	34999
35017	35020	35022	35041	35066	35071	35098	35140	35149	35174	35192	35226
35252	35293	35310	35347	35350	35376	35387	35485	35528	35536	35547	35556
35574	35594	35638	35667	35675	35704	35727	35801	35815	35837	35879	35902
35936	36023	36052	36074	36098	36102	36111	36116	36129	36130	36151	36161
36199	36221	36232	36234	36242	36244	36247	36248	36251	36254	36258	36265
36268	36269	36271	36275	36278	36291	36296	36303	36333	36336	36338	36341
36347	36364	36372	36374	36392	36406	36411	36419	36427	36432	36439	36441
36442	36443	36462	36466	36472	36518	36526	36543	36549	36554	36572	36573
36575	36585	36602	36604	36618	36627	36632	36635	36638	36641	36642	36648
36652	36653	36659	36664	36679	36685	36692	36694	36753	36764	36769	36770
36771	36775	36780	36790	36801	36804	36805	36819	36820	36828	36846	36849
36865	36895	36910	36914	36918	36925	36930	36935	36957	36960	36977	36987
36989	37002	37029	37050	37059	37061	37063	37070	37085	37086	37089	37091
37094	37099	37100	37101	37102	37132	37151	37169	37174	37176	37177	37182
37187	37191	37200	37225	37226	37230	37257	37259	37267	37268	37270	37277
37280	37293	37294	37296	37322	37327	37340	37370	37395	37413	37417	37424
37460	37463	37495	37527	37544	37606	37634	37642	37658	37672	37746	37771
37843	37941	37967	37970	37980	37996	38000	38002	38005	38074	38077	38115
38160	38161	38169	38219	38227	38239	38293	38348	38389	38415	38418	38429
38554	38694	38741	38766	38780	38790	38808	38825	38871	38895	38898	39164
39190	39199	39235	39246	39288	39322	39495	39534	39562	39571	39607	39608
39619	39657	39697	39709	39727	39731	39876	39913	39937	39944	39974	39976
39986	40051	40069	40118	40191	40195	40211	40230	40232	40269	40325	40334
40379	40420	40474	40524	40627	40671	40694	40735	40760	40778	40794	40795
40842	40876	40879	40913	40918	40958	40971	41035	41085	41090	41092	41115
41163	41166	41169	41186	41190	41211	41224	41332	41363	41391	41483	41496
41532	41658	41692	41700	41744	41747	41796	41797	41803	41824	41835	41863
41871	41877	41879	41882	41885	41898	41902	41903	41905	41912	41916	41931
41940	41943	41944	41945	41955	41971	41975	42037	42080	42111	42117	42137
42168	42224	42225	42229	42230	42231	42246	42253	42256	42266	42276	42281
42289	42299	42325	42332	42351	42353	42357	42363	42364	42372	42373	42376
42389	42442	42458	42462	42505	42510	42584	42619	42626	42681	42684	42720
42721	42731	42761	42813	42826	42833	42839	42853	42863	42905	42949	42997
43006	43017	43068	43102	43104	43120	43182	43209	43214	43235	43252	43263
43268	43303	43327	43342	43343	43349	43377	43518	43546	43622	43626	43634
43645	43659	43671	43680	43737	43789	43796	43828	43830	43872	43890	43943
43968	44000	44001	44003	44005	44025	44026	44032	44039	44040	44046	44058
44064	44070	44074	44137	44169	44173	44214	44234	44240	44241	44244	44268
44330	44393	44394	44408	44433	44474	44504	44513	44538	44597	44607	44633
44762	44764	44810	44817	44908	44934	44956	44991	45009	45035	45039	45046
45075	45076	45094	45096	45101	45123	45124	45138	45139	45144	45156	45169
45177	45208	45209	45211	45225	45262	45267	45273	45281	45282	45293	45296
45308	45321	45322	45323	45350	45352	45353	45355	45382	45466	45500	45503
45504	45555	45561	45575	45577	45586	45589	45591	45594	45597	45610	45611
45614	45618	45627	45636	45642	45644	45651	45654	45657	45663	45664	45665
45668	45672	45676	45682	45684	45692	45712	45717	45729	45746	45755	45756
45760	45767	45771	45774	45779	45790	45791	45792	45795	45820	45829	45836
45837	45838	45840	45858	45860	45861	45866	45876	45886	45898	45908	45915
45922	45933	45934	45949	45959	45963	45972	45975	45979	45991	45998	45999
46004	46005	46006	46014	46025	46030	46036	46041	46043	46047	46051	46074
46108	46109	46110	46113	46114	46116	46117	46121	46122	46124	46126	46136
46140	46143	46144	46149	46175	46187	46189	46192	46198	46200	46207	46213
46217	46218	46235	46243	46246	46248	46249	46251	46265	46282	46291	46303
46305	46311	46312	46314	46328	46343	46356	46360	46361	46373	46382	46389
46390	46391	46399	46405	46412	46421	46428	46429	46432	46434	46458	46465
46471	46472	46489	46493	46498	46505	46507	46516	46519	46525	46528	46529
46547	46553	46556	46571	46579	46582	46616	46626	46629	46637	46647	46648
46661	46725	46726	46782	46785	46815	46821	46870	46872	46917	46927	46934
46936	46948	46963	46971	47037	47110	47122	47145	47160	47222	47230	47251
47293	47345	47367	47372