Diaryl ether derivative, addition salt thereof, and immunosuppressant

[0001] This is a 371 of PCT/JP02/09864, filed Sep. 25, 2002.

[0002] The present invention relates to diaryl ether derivatives, salts and hydrates thereof that are useful as an immunosuppressive agent.

[0003] Immunosuppressive agents are widely used as a treatment for autoimmune diseases such as rheumatoid arthritis, nephritis, osteoarthritis and systemic lupus erythematosus, chronic inflammatory diseases such as inflammatory bowel disease, and allergic diseases such as asthma and dermatitis. Progress in medicine has led to an increase in the number of tissue and organ transplantations performed each year. In such a situation of modern medicine, having as much control as possible over the rejection following transplantation is a key to successful transplantation. Immunosuppressive agents also play a significant role to this end.

[0004] Among immunosuppressors commonly used in organ transplantation are antimetabolites, such as azathioprine and mycophenolate mofetil, calcineurin inhibitors, such as cyclosporin A and tacrolimus, and corticosteroid, such as prednisolone. Some of these drugs are not effective enough while others require continuous monitoring of the blood drug level to avoid renal failure and other serious side effects. Thus, none of conventional immunosuppressive agents are satisfactory in view of efficacy and potential side effects.

[0005] Multiple drug combined-therapy, in which different immunosuppressive drugs with different mechanisms of action are used, is becoming increasingly common with the aims of alleviating the side effects of the drugs and achieving sufficient immunosuppressive effects. Also, development of new types of immunosuppressive agents that have completely different mechanisms of action is sought.

[0006] In an effort to respond to such demands, the present inventors conducted a search for new types of immunosuppressive agents with main emphasis on 2-amino-1,3-propanediol derivatives.

[0007] While the use of 2-amino-1,3-propanediol derivatives as immunosuppressive agents has been disclosed in PCT publication WO94/08943 (YOSHITOMI PHARMACEUTICAL INDUSTRIES, Ltd., TAITO Co., Ltd.) and in Japanese Patent Publication No. Hei 9-2579602 (YOSHITOMI PHARMACEUTICAL INDUSTRIES, Ltd., TAITO Co., Ltd.), it has not been previously known that 2-amino-1,3-propanediol derivatives having a diaryl ether group, which are subjects of the present invention, can serve as an effective immunosuppressor.

[0008] Accordingly, it is an objective of the present invention to provide a diaryl ether derivative that exhibits significant immunosuppressive effects with little side effects.

[0009] In the course of studies on immunosuppressive agents that have different mechanisms of action from antimetabolites and calcineurin inhibitors, the present inventors discovered that novel diaryl ether derivatives that have a different structure from conventional immunosuppressors exhibit strong immunosuppressive effects. Specifically, the compounds are such that one of the aryl groups includes, at its para-position, a carbon chain with an aminopropanediol group and the other aryl group includes a substituent at its meta-position. This discovery led the present inventors to devise the present invention.

[0010] The present invention thus is an immunosuppressive agent containing as an active ingredient at least one of a diaryl ether derivative, a pharmaceutically acceptable salt and hydrate thereof, the diaryl ether derivative represented by the following general formula (1):
wherein R₁ is halogen, trihalomethyl, hydroxy, lower alkyl having 1 to 7 carbon atoms, substituted or unsubstituted phenyl, aralkyl, lower alkoxy having 1 to 4 carbon atoms, trifluoromethyloxy, phenoxy, cyclohexylmethyloxy, substituted or unsubstituted aralkyloxy, pyridylmethyloxy, cinnamyloxy, naphthylmethyloxy, phenoxymethyl, hydroxymethyl, hydroxyethyl, lower alkylthio having 1 to 4 carbon atoms, lower alkylsulfinyl having 1 to 4 carbon atoms, lower alkylsulfonyl having 1 to 4 carbon atoms, benzylthio, acetyl, nitro, or cyano; R₂ is hydrogen, halogen, trihalomethyl, lower alkoxy having 1 to 4 carbon atoms, lower alkyl having 1 to 7 carbon atoms, phenethyl, or benzyloxy; R₃ is hydrogen, halogen, trifluoromethyl, lower alkoxy having 1 to 4 carbon atoms, hydroxy, benzyloxy, lower alkyl having 1 to 7 carbon atoms, phenyl, lower alkoxymethyl having 1 to 4 carbon atoms, or lower alkylthio having 1 to 4 carbon atoms; and X is —(CH₂)_n— (n is an integer from 1 to 4), —OCH₂CH₂—, or —CH═CHCH₂—.

[0011] More specifically, the present invention is an immunosuppressive agent containing as an active ingredient at least one of a diaryl ether derivative, a pharmaceutically acceptable salt and hydrate thereof, the diaryl ether derivative represented by the following general formula (1a):
wherein R₂, R₃, and X are the same as defined above.

[0012] Furthermore, the present invention is an immunosuppressive agent containing as an active ingredient at least one of a diaryl ether derivative, a pharmaceutically acceptable salt and hydrate thereof, the diaryl ether derivative represented by the following general formula (1b):
wherein R₂, R₃, and X are the same as defined above; and R₄ is hydrogen, halogen, trifluoromethyl, lower alkoxy having 1 to 4 carbon atoms, or lower alkyl having 1 to 7 carbon atoms.

[0021] The compounds of the general formulae (1), (1a) and (1b) are novel compounds. Examples of the pharmaceutically acceptable salt of the compound of the general formula (1) include acid salts, such as hydrochloride, hydrobromide, acetate, trifluoroacetate, methanesulfonate, citrate, and tartrate.

[0022] In the general formula (1), the term ‘halogen atom’ includes fluorine, chlorine, bromine, and iodine atom. The term ‘trihalomethyl group’ includes trifluoromethyl and trichloromethyl. The phrase ‘lower alkyl group having 1 to 7 carbon atoms’ includes straight-chained or branched hydrocarbons having 1 to 7 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, t-butyl, pentyl, hexyl, and heptyl. The phrase ‘substituted or unsubstituted phenoxy group’ includes those that have, at any position of its benzene ring, a halogen atom, such as fluorine, chlorine, bromine and iodine, trifluoromethyl, lower alkyl having 1 to 4 carbon atoms, or lower alkoxy having 1 to 4 carbon atoms. The term ‘aralkyl group’ as in ‘aralkyl group’ or ‘aralkyloxy group’ includes benzyl, diphenylmethyl, phenethyl, and phenylpropyl. The term ‘lower alkyl group’ as used in ‘lower alkoxyl group having 1 to 4 carbon atoms,’ ‘lower alkylthio group having 1 to 4 carbon atoms,’ ‘lower alkylsulfinyl group having 1 to 4 carbon atoms,’ or ‘lower alkylsulfonyl group having 1 to 4 carbon atoms,’ includes straight-chained or branched hydrocarbons having 1 to 4 carbon atoms, such as methyl, ethyl, propyl, isopropyl, and butyl. The phrase ‘substituted or unsubstituted aralkyl group’ includes those that have, at any position of its benzene ring, a halogen atom, such as fluorine, chlorine, bromine and iodine, trifluoromethyl, lower alkyl having 1 to 4 carbon atoms, or lower alkoxy having 1 to 4 carbon atoms.

[0023] According to the present invention, the compounds of the general formula (1) can be produced in the following pathways:

[0024] The compound appearing in the synthetic pathway 1 and represented by the following general formula (3):
(wherein R₅ is lower alkyl having 1 to 4 carbon atoms; Boc is t-butoxycarbonyl; and R₁, R₂, R₃, and X are the same as described above) can be prepared by reacting a compound of the following general formula (2):
(wherein Y is chlorine, bromine, or iodine; and R₁, R₂, R₃, and X are as described above) with a compound of the following general formula (11):
(wherein R₅ and Boc are as described above) in the presence of a base (Step 1).

[0025] This reaction can be carried out using a reaction solvent such as 1,4-dioxane, dimethylsulfoxide (DMSO), N,N-dimethylformamide (DMF), tetrahydrofuran (THF), or ethanol at a reaction temperature of 0° C. to reflux temperature, preferably at a temperature of 80° C. to 100° C., in the presence of an inorganic base such as sodium hydride, potassium hydride, sodium alkoxide, and potassium alkoxide.

[0026] The compound appearing in the synthetic pathway 1 and represented by the following general formula (4):
(wherein R₁, R₂, R₃, R₄, X, and Boc are as described above) can be prepared by the reduction of the compound of the general formula (3) (Step 2).

[0027] This reaction can be carried out at a reaction temperature of 0° C. to reflux temperature, preferably at room temperature, using an alkylborane derivative, such as borane (BH₃) and 9-borabicyclo[3.3.1]nonane (9-BBN), or a metal hydride complex, such as diisobutylaluminum hydride ((iBu)2AlH), sodium borohydride (NaBH₄) and lithium aluminum hydride (LiAlH₄), preferably lithium borohydride (LiBH₄), and using a reaction solvent such as THF, ethanol and methanol.

[0028] The compound appearing in the synthetic pathway 1 and represented by the general formula (1):
(wherein R₁, R₂, R₃, and X are as described above) can be prepared by the acidolysis of the compound of the general formula (4) (Step 3).

[0029] This reaction can be carried out at a reaction temperature in the range of 0° C. to room temperature in an inorganic or organic acid, such as acetic acid, hydrochloric acid, hydrobromic acid, methanesulfonic acid and trifluoroacetic acid, or in a mixed solvent with an organic solvent such as methanol, ethanol, THF, 1,4-dioxane, and ethyl acetate.

[0030] The compound appearing in the synthetic pathway 2 and represented by the following general formula (6):
(wherein R₃, R₅, X, and Boc are as described above) can be prepared by reacting the compound represented by the following general formula (5):
(wherein R₃, X, and Y are as described above) with the compound of the general formula (11):
(wherein R₅, and Boc are as described above) in the presence of a base (Step 4).

[0031] This reaction can be carried out using a reaction solvent such as 1,4-dioxane, DMSO, DMF, THF, or ethanol at a reaction temperature in the range of 0° C. to reflux temperature, preferably 80° C. to 100° C., in the presence of an inorganic base such as sodium hydride, potassium hydride, sodium alkoxide, and potassium alkoxide.

[0032] The compound appearing in the synthetic pathway 2 and represented by the following general formula (7):
(where R₃ and X are as described above) can be prepared by the reduction of the compound of the general formula (6) (Step 5).

[0033] This reaction can be carried out at a reaction temperature of 0° C. to reflux temperature, preferably at room temperature, using an alkylborane derivative, such as BH₃ and 9-BBN, or a metal hydride complex, such as (iBu)₂AlH, NaBH₄ and LiAlH₄, preferably LiBH₄, and using a reaction solvent such as THF, ethanol, and methanol.

[0034] The compound appearing in the synthetic pathway 2 and represented by the following general formula (8):
(wherein M is carbon or silicon; R6 and R7 are each independently hydrogen or lower alkyl having 1 to 4 carbon atoms; and R₃, X and Boc are as described above) can be prepared by reacting the compound of the general formula (7) with a compound of the general formula (12):
(where R₆ and R₇ are as described above) or a compound of the general formula (13):
(wherein R₈ is lower alkyl having 1 to 4 carbon atoms; and R₆ and R₇ are as described above) or a compound of the general formula (14):
(wherein R₉ is chlorine or trifluoromethansulfonyloxy; and R₆ and R₇ are as described above) (Step 6).

[0035] The reaction between the compound of the general formula (7) and the compound of the general formula (12) or the compound of the general formula (13) can be carried out at a reaction temperature of room temperature to 100° C. either in the presence of a Lewis acid such as zinc chloride or in the presence of an acid catalyst such as camphorsulfonic acid, paratoluenesulfonic acid, and pyridinium paratoluenesulfonic acid, and either in the absence of solvent or in the presence of a reaction solvent such as DMF, THF, and methylene chloride.

[0036] The reaction between the compound of the general formula (7) and the compound of the general formula (14) can be carried out at a reaction temperature of 0° C. to 100° C. in the presence of a base such as triethylamine, pyridine, 2,6-lutidine, and imidazole, using a reaction solvent such as DMF, THF, methylene chloride, chloroform, and acetonitrile.

[0037] The compound appearing in the synthetic pathway 2 and represented by the general formula (9):
(wherein R₃, R₆, R₇, X, Boc, and M are as described above) can be prepared by the hydrogenolysis of the compound of the general formula (8) (Step 7).

[0038] This reaction can be carried out at a temperature in the range of room temperature to 100° C. in the presence of a reduction catalyst, such as palladium carbon, platinum carbon, platinum oxide, rhodium carbon, and ruthenium carbon, in a solvent, such as ethanol, methanol, THF, DMF, and ethyl acetate, under a hydrogen pressure that is atmospheric pressure or higher.

[0039] The compound appearing in the synthetic pathway 2 and represented by the general formula (10):
[wherein R₁, R₂, R₃, R₆, R₇, X, Boc, and M are as described above] can be prepared by reacting the compound of the general formula (9) with a compound of the general formula (15):
(wherein R₁ and R₂ are as described above) in the presence of copper acetate (Step 8).

[0040] This reaction can be carried out at room temperature in the presence or absence of a molecular sieve, using copper acetate as a reaction promoter and methylene chloride or chloroform as a solvent, in the presence of a base, such as triethylamine.

[0041] The compound appearing in the synthetic pathway 2 and represented by the general formula (1):
(wherein R1, R2, R3, and X are as described above) can be prepared by the acidolysis, or desilylation followed by acidolysis, of the compound of the general formula (10) (Step 9).

[0042] This reaction can be carried out at a reaction temperature of 0° C. to room temperature in an inorganic or organic acid, such as acetic acid, hydrochloric acid, hydrobromic acid, methanesulfonic acid, trifluoroacetic acid, or in a mixed solution with an organic solvent, such as methanol, ethanol, THF, 1,4-dioxane, and ethyl acetate.

[0043] When M in the general formula (10) is a silicon atom, the compound of the general formula (1) can be synthesized by reacting potassium fluoride, cesium fluoride, or tetrabutylammonium fluoride at a temperature of 0° C. to room temperature in a solvent such as THF, DMF, and 1,4-dioxane and then subjecting the resulting compound to the above-described acidolysis.

[0044] Of the compounds of the general formula (10), those represented by the general formula (16) in which R₁ is a substituted or unsubstituted aralkyloxy group:
(wherein R₁₀ is substituted or unsubstituted aralkyl; and R₂, R₃, R₆, R₇, X, Boc, and M are as described above) can also be prepared by reacting a compound of the general formula (17):
(wherein R₂, R₃, R₆, R₇, X, Boc, and M are as described above) with a compound of the general formula (18):
R₁₀Y′  (18)
(wherein Y′ is halogen or hydroxy; and R₁₀ is as described above).

[0045] When Y′ is a halogen atom, the reaction can be carried out at a reaction temperature in the range of room temperature to 80° C., using an organic base, such as triethylamine, and pyridine, or an inorganic base, such as sodium hydride, sodium carbonate, and potassium carbonate, and using a reaction solvent, such as THF, DMF, and 1,4-dioxane.

[0046] When Y′ is a hydroxy, the reaction can be carried out at room temperature in the presence of diethyl azodicarboxylate or triphenylphosphine, using THF as a solvent.

[0047] The compound of the general formula (17) can be prepared by the hydrogenolysis of a compound of the general formula (19):
(wherein R₂, R₃, R₆, R₇, X, Boc, and M are as described above).

[0048] This reaction can be carried out at a temperature in the range of room temperature to 100° C. in the presence of a reduction catalyst, such as palladium carbon, platinum carbon, platinum oxide, rhodium carbon, and ruthenium carbon, in a solvent, such as ethanol, methanol, THF, DMF, and ethyl acetate, under a hydrogen pressure that is atmospheric pressure or higher.

[0049] Of the compounds represented by the general formula (10), those represented by the general formula (20) in which R₁ is a substituted or unsubstituted phenoxy group:
(wherein R₁₁ is hydrogen, halogen, trifluoromethyl, lower alkyl having 1 to 4 carbon atoms, or lower alkoxy having 1 to 4 carbon atoms; and R₂, R₃, R₆, R₇, X, Boc, and M are as described above) can be prepared by reacting the compound of the general formula (17) with a compound of the general formula (21):
(wherein R₁₁ is as described above) in the presence of copper acetate.

[0050] This reaction can be carried out preferably at room temperature in the presence or absence of a molecular sieve, using copper acetate as a reaction promoter and methylene chloride or chloroform as a solvent, in the presence of a base, such as triethylamine.

[0051] The present invention will now be described with reference to examples, which are not intended to limit the scope of the invention in any way.

[0052] 4-(3-benzyloxyphenoxy)-2-chlorobenzaldehyde

[0053] Potassium carbonate (5.53 g) was added to a DMF solution (70 ml) of 2-chloro-4-fluorobenzaldehyde (3.35 g) and 3-benzyloxyphenol (4.23 g) and the solution was stirred for 3 hours while heated to 150° C. The reaction mixture was decanted into water and was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=6:1). In this manner, the desired product (6.73 g) was obtained as a colorless powder.

[0054] Using various phenol derivatives and aldehydes, compounds shown Table 1 were synthesized in the same manner as in Reference Example 1 above.

[0055]

TABLE 1
Reference
Example	R1	R2	R3	R4
2	CF3	H	H	H
3	CF3	H	MeO	H
4	CF3	H	H	MeO
5	CF3	H	Cl	H
6	CF3	H	H	Cl
7	CF3	H	H	PhCH2O
8	CF3	H	CF3	H
9	CF3	H	H	CF3
10	CF3	CF3	H	Cl
11	CF3	Ph(CH2)2	H	H
12	Ph(CH2)2	Ph(CH2)2	H	H
13	Ph(CH2)2	H	H	Cl
14	Ph(CH2)2	H	H	CF3
15	Ph(CH2)2	Ph(CH2)2	H	Cl
16	Ph(CH2)2	Ph(CH2)2	H	CF3
17	PhCH2O	H	H	H
18	PhCH2O	PhCH2O	H	H
19	PhCH2O	H	H	i-Pr
20	PhCH2O	PhCH2O	H	Cl
21	PhCH2O	Cl	H	Cl
22	PhCH2O	H	H	Br
23	PhCH2O	H	H	CF3
24	PhCH2O	H	H	Ph
25	MeO	CF3	H	H
26	MeO	CF3	H	Cl
27	t-Bu	H	H	H
28	MeS	H	H	H
29	n-C5H11	H	H	H
30	n-C7H15	H	H	H
31	i-Pr	i-PrO	H	H
32	i-Pr	i-PrO	H	Cl
33	i-Pr	i-Pr	H	Cl
34	Cl	Cl	H	Cl
35	PhCH2S	H	H	H
36	PhCH2S	H	H	Cl
37	Me	H	H	H

[0056] 2-fluoro-4-[(3-trifluoromethyl)phenoxy]benzaldehyde

[0057] 3-(trifluoromethyl)phenylboric acid (1.03 g) and 2-fluoro-4-hydroxybenzaldehyde (760 mg) were dissolved in methylene chloride. While the solution was stirred, copper acetate (985 mg), molecular sieve 4A (800 mg), and triethylamine (3.76 mL) were added. After 6 and 24 hours, the same amount of copper acetate was added and the mixture was stirred for additional 48 hours. The insoluble material was then filtered out and the filtrate was decanted into water and was extracted with ethyl acetate. The organic phase was washed with water and then with a saturated aqueous solution of sodium chloride and was dried with anhydrous magnesium sulfate. Subsequently, the solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=7:1, and then 2:1). In this manner, the desired product (265 mg) was obtained as a yellow oil.

[0058] Ethyl 4′-(3-benzyloxyphenoxy)-2′-chlorocinnamate

[0059] Under argon, 60% sodium hydride (960 mg) was added to a THF solution (150 ml) of ethyl (diethylphosphono)acetate (4.8 mL) at 0° C. and the mixture was stirred for 30 minutes. A THF solution (20 mL) of the compound of Reference Example 1 (6.73 g) was then added dropwise. With the temperature maintained, the mixture was further stirred for 1 hour, followed by addition of water and then extraction with ethyl acetate. The organic phase was washed with water and then with a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=6:1). In this manner, the desired product (7.36 g) was obtained as a colorless oil.

[0060] Using the compounds of Reference Examples 2 through 38, the compounds shown in Table 2 below were synthesized in the same manner as in Reference Example 39 above.

[0061]

TABLE 2
Reference
Example	R1	R2	R3	R4
40	CF3	H	H	H
41	CF3	H	MeO	H
42	CF3	H	H	MeO
43	CF3	H	Cl	H
44	CF3	H	H	Cl
45	CF3	H	H	PhCH2O
46	CF3	H	CF3	H
47	CF3	H	H	CF3
48	CF3	CF3	H	Cl
49	CF3	Ph(CH2)2	H	H
50	Ph(CH2)2	Ph(CH2)2	H	H
51	Ph(CH2)2	H	H	Cl
52	Ph(CH2)2	H	H	CF 3
53	Ph(CH2)2	Ph(CH2)2	H	Cl
54	Ph(CH2)2	Ph(CH2)2	H	CF3
55	PhCH2O	H	H	H
56	PhCH2O	PhCH2O	H	H
57	PhCH2O	PhCH2O	H	Cl
58	PhCH2O	H	H	i-Pr
59	PhCH2O	Cl	H	Cl
60	PhCH2O	H	H	Br
61	PhCH2O	H	H	CF3
62	PhCH2O	H	H	Ph
63	MeO	CF3	H	H
64	MeO	CF3	H	Cl
65	t-Bu	H	H	H
66	MeS	H	H	H
67	n-C5H11	H	H	H
68	n-C7H15	H	H	H
69	i-Pr	i-PrO	H	H
70	i-Pr	i-PrO	H	Cl
71	i-Pr	i-Pr	H	Cl
72	Cl	Cl	H	Cl
73	PhCH2S	H	H	H
74	PhCH2S	H	H	Cl
75	CF3	H	H	F
76	Me	H	H	H

[0062] Methyl 4′-(3-isobutylphenoxy)cinnamate

[0063] Potassium carbonate (622 mg) was added to a DMF solution (10 ml) of 3-isobutylphenol (451 mg) and methyl 4′-fluorocinnamate (541 mg), and the solution was stirred for 8 hours while heated to 140° C. The reaction mixture was decanted into water and was extracted with ethyl acetate. The organic phase was washed with water and then with a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=30:1). In this manner, the desired product (278 mg) was obtained as a yellow oil.

[0064] Methyl 4′-(3-ethylphenoxy)cinnamate

[0065] Using 3-ethylphenol and methyl 4′-fluorocinnamate, reactions were carried out in the same manner as in Reference Example 77 above. The desired product was obtained as a yellow oil.

[0066] Ethyl 4′-[(3-phenoxymethyl)phenoxy]cinnamate

[0067] The compound of Reference Example 76 (2.82 g) was dissolved in carbon tetrachloride (50 mL). Following addition of N-bromosuccinimide (2.31 g), the solution was stirred under exposure to light while heated. After 24 hours, the solvent was removed by distillation under reduced pressure, and the residue was extracted with ethyl acetate. The organic phase was washed with water and then with a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=6:1). In this manner, ethyl 4′-[(3-bromomethyl)phenoxy]cinnamate (1.30 g) was obtained as a yellow oil. To a DMF solution (25 mL) of the resulting bromide (1.24 g), phenol (380 mg) and potassium carbonate (500 mg) were added, and the mixture was stirred for 3 hours at 60° C. Subsequently, the reaction mixture was decanted into water and was extracted with ethyl acetate. The organic phase was washed with water and then with a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=4:1). In this manner, the desired product (1.30 g) was obtained as a colorless oil.

[0068] Ethyl 4′-[(3-benzyloxy)phenoxy]-2′-chlorodihydrocinnamate

[0069] The compound of Reference Example 39 (7.36 g) was dissolved in ethanol (100 mL). While the solution was stirred at 0° C., bismuth chloride (2.84 g) was added. Sodium borohydride (2.72 g) was then added in three portions and the mixture was subsequently stirred for 3 hours at room temperature. Ice water was then added to the reaction mixture and the crystallized inorganic deposits were filtered out through celite. The resulting filtrate was extracted with ethyl acetate. The organic phase was washed with water and then with a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure. In this manner, the desired product (7.40 g) was obtained as a colorless oil (Method A).

[0070] Methyl 4′-(3-isobutylphenoxy)dihydrocinnamate

[0071] The compound of Reference Example 77 (278 mg) was dissolved in ethanol (5 mL), and 10% Pd/C (70.0 mg) was added to the solution. The resulting mixture was then stirred for 2 hours at room temperature under hydrogen. The catalyst was filtered out and the filtrate was concentrated under reduced pressure to obtain the desired product as a colorless oil (Method B).

[0072] Methyl 4′-(3-ethylphenoxy)dihydrocinnamate

[0073] Using the compound of Reference Example 78, reactions were carried out in the same manner as in Reference Example 81 above. In this manner, the desired product was obtained as a colorless oil.

[0074] Ethyl 3′-chloro-4′-[(3-trifluoromethyl)phenoxy]dihydrocinnamate

[0075] The compound of Reference Example 43 (2.29 g) was dissolved in ethyl acetate (30 mL), and 5% Pd/C-ethylenediamine complex (230 mg) was added to the solution. The resulting mixture was then stirred for 3.5 hours at room temperature under hydrogen. The catalyst was then filtered out and the filtrate was concentrated under reduced pressure to obtain the desired product (2.30 g) as a pale yellow oil (Method C).

[0076] Using the compounds of Reference Examples 40 through 42, 44 through 65, 67 through 75, and 79, reactions were carried out in the same manner as in Reference Examples 80 through 83 above to synthesize compounds as shown in Table 3 below.

[0077]

TABLE 3
Reference
Example	R1	R2	R3	R4	Process
84	CF3	H	H	H	B
85	CF3	H	MeO	H	B
86	CF3	H	H	MeO	B
87	CF3	H	H	Cl	C
88	CF3	H	H	PhCH2O	C
89	CF3	H	CF3	H	B
90	CF3	H	H	CF3	B
91	CF3	CF3	H	Cl	A
92	CF3	Ph(CH2)2	H	H	B
93	Ph(CH2)2	Ph(CH2)2	H	H	B
94	Ph(CH2)2	H	H	Cl	A
95	Ph(CH2)2	H	H	CF3	B
96	Ph(CH2)2	Ph(CH2)2	H	Cl	A
97	Ph(CH2)2	Ph(CH2)2	H	CF3	B
98	PhCH2O	H	H	H	A
99	PhCH2O	PhCH2O	H	H	A
100	PhCH2O	H	H	i-Pr	A
101	PhCH2O	PhCH2O	H	Cl	A
102	PhCH2O	Cl	H	Cl	A
103	PhCH2O	H	H	Br	A
104	PhCH2O	H	H	CF3	A
105	PhCH2O	H	H	Ph	A
106	MeO	CF3	H	H	A
107	MeO	CF3	H	Cl	A
108	t-Bu	H	H	H	B
109	n-C5H11	H	H	H	B
110	n-C7H15	H	H	H	B
111	i-Pr	i-PrO	H	H	B
112	i-Pr	i-PrO	H	Cl	C
113	i-Pr	i-Pr	H	Cl	C
114	Cl	Cl	H	Cl	A
115	PhCH2S	H	H	H	A
116	PhCH2S	H	H	Cl	A
117	PhOCH2	H	H	H	A
118	CF3	H	H	F	B

[0078] Ethyl 4′-[(3-t-butyldimethylsiloxy)phenoxy]-2′-chloro-dihydrocinnamate

[0079] Using the compound of Reference Example 39, reactions were carried out in the same manner as in Reference Example 83 (Method C). The resulting phenol (7.10 g) was dissolved in DMF (80 mL), and imidazole (1.80 g) and t-butyldimethylchlorosilane (3.98 g) were added to the solution. The mixture was then stirred overnight at room temperature. Subsequently, the mixture was decanted into water and was extracted with ethyl acetate. The organic phase was washed with water and then with a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=4:1). In this manner, the desired product (8.86 g) was obtained as a colorless oil.

[0080] Methyl 4′-[(3-methylthio)phenoxy]dihydrocinnamate

[0081] Under argon, the compound of Reference Example 66 (4.07 g) was dissolved in methanol (50 mL). While the solution was stirred at 10° C., magnesium (1.00 g) was added to the solution. With the temperature maintained, the mixture was further stirred for 3 hours, followed by addition of diluted hydrochloric acid and then extraction with ethyl acetate. The organic phase was washed with water and then with a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure to obtain the desired product (3.70 g) as a colorless oil.

[0082] Benzyl 4′-[3-benzyloxy-5-(trifluoromethyl)phenoxy]dihydrocinnamate

[0083] The compound of Reference Example 106 (840 mg) was dissolved in methylene chloride (20 mL). While the solution was stirred at 0° C., a 1 mol/L boron tribromide-methylene chloride solution (3.42 mL) was added dropwise. Subsequently, the mixture was stirred overnight at room temperature. Ice water was then added to the reaction mixture and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure. In this manner, 4′-(3-trifluoromethyl-5-hydroxyphenoxy)dihydrocinnamate (750 mg) was obtained as a light brown powder. The powder so produced was dissolved in DMF (50 mL), followed by the addition of potassium carbonate (1.04 g) and benzyl bromide (0.602 mL). The mixture was then stirred at room temperature for 8 hours, decanted into ice water, and extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was then dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure to obtain the desired product as a brown oil.

[0084] Ethyl 4′-[3-benzyloxy-5-(trifluoromethyl)phenoxy]-2′-chlorodihydrocinnamate

[0085] Using the compound of Reference Example 107, 2′-chloro-4′-(3-trifluoromethyl-5-hydroxyphenoxy)dihydrocinnamic acid was obtained in the same manner as in Reference Example 121 above. The cinnamic acid (1.47 g) so obtained was dissolved in ethanol (10 mL). While the solution was stirred at 0° C., thionyl chloride (3 mL) was added dropwise. With the temperature maintained, the solution was stirred for additional 2 hours. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=10:1 and then 6:1). As a result, ethyl 2′-chloro-4′-(3-trifluoromethyl-5-hydroxyphenoxy)dihydrocinnamate (1.38 g) was obtained as a colorless oil. Using potassium carbonate and benzyl bromide, the resultant ester was subjected to benzyl-etherification as with Reference Example 121 above. In this manner, the desired product was obtained as a colorless oil.

[0086] 4′-[(3-benzyloxy)phenoxy]-2′-chlorodihydrocinnamyl alcohol

[0087] The compound of Reference Example 80 (7.40 g) was dissolved in THF (100 mL). While the solution was stirred at 0° C., lithium aluminum hydride (500 mg) was added. After 10 minutes, a 20% aqueous solution of NaOH was added and the crystallized insoluble inorganic deposits were filtered out through celite. The filtrate was then extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure to obtain the desired product (6.37 g) as a colorless oil.

[0088] Using the compounds of Reference Examples 81 through 105 and 108 through 122, the compounds shown in Table 4 below were synthesized in the same manner as in Reference Example 123 above.

[0089]

TABLE 4
	Reference
	Example	R1	R2	R3	R4
124	CF3	H	H	H
125	CF3	H	MeO	H
126	CF3	H	H	MeO
127	CF3	H	Cl	H
128	CF3	H	H	Cl
129	CF3	H	H	PhCH2O
130	CF3	H	CF3	H
131	CF3	H	H	CF3
132	CF3	CF3	H	Cl
133	CF3	Ph(CH2)2	H	H
134	CF3	H	H	F
135	Ph(CH2)2	Ph(CH2)2	H	H
136	Ph(CH2)2	H	H	Cl
137	Ph(CH2)2	H	H	CF3
138	Ph(CH2)2	Ph(CH2)2	H	Cl
139	Ph(CH2)2	Ph(CH2)2	H	CF3
140	PhCH2O	H	H	H
141	PhCH2O	PhCH2O	H	H
142	tBuMe2SiO	H	H	Cl
143	PhCH2O	H	H	i-Pr
144	PhCH2O	PhCH2O	H	Cl
145	PhCH2O	Cl	H	Cl
146	PhCH2O	H	H	Br
147	PhCH2O	H	H	CF3
148	PhCH2O	H	H	Ph
149	PhCH2O	CF3	H	H
150	PhCH2O	CF3	H	Cl
151	t-Bu	H	H	H
152	MeS	H	H	H
153	n-C5H11	H	H	H
154	n-C7H15	H	H	H
155	i-Pr	i-PrO	H	H
156	i-Pr	i-PrO	H	Cl
157	i-Pr	i-Pr	H	Cl
158	Cl	Cl	H	Cl
159	PhCH2S	H	H	H
160	PhCH2S	H	H	Cl
161	Et	H	H	H
162	i-Bu	H	H	H
163	PhOCH2	H	H	H

[0090] 4′-[(3-benzyloxy)phenoxy]-2′-chlorodihydrocinnamyl iodide

[0091] The compound of Reference Example 123 (6.37 g) was dissolved in THF (150 mL). While the solution was stirred at 0° C., imidazole (2.45 g), triphenylphosphine (9.44 g), and iodine (9.14 g) were added. With the temperature maintained, the solution was further stirred for 1 hour. Subsequently, water was added to the reaction mixture and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=20:1). In this manner, the desired product (7.90 g) was obtained as a colorless oil.

[0092] Using the compounds of Reference Examples 124 through 163, the compounds shown in Table 5 below were synthesized in the same manner as in Reference Example 164 above.

[0093]

TABLE 5
Reference
Example	R1	R2	R3	R4
165	CF3	H	H	H
166	CF3	H	MeO	H
167	CF3	H	H	MeO
168	CF3	H	Cl	H
169	CF3	H	H	Cl
170	CF3	H	H	PhCH2O
171	CF3	H	CF3	H
172	CF3	H	H	CF3
173	CF3	CF3	H	Cl
174	CF3	Ph(CH2)2	H	H
175	CF3	H	H	F
176	Ph(CH2)2	Ph(CH2)2	H	H
177	Ph(CH2)2	H	H	Cl
178	Ph(CH2)2	H	H	CF3
179	Ph(CH2)2	Ph(CH2)2	H	Cl
180	Ph(CH2)2	Ph(CH2)2	H	CF3
181	PhCH2O	H	H	H
182	PhCH2O	PhCH2O	H	H
183	t-BuMe2SiO	H	H	Cl
184	PhCH2O	H	H	i-Pr
185	PhCH2O	PhCH2O	H	Cl
186	PhCH2O	Cl	H	Cl
187	PhCH2O	H	H	Br
188	PhCH2O	H	H	CF3
189	PhCH2O	H	H	Ph
190	PhCH2O	CF3	H	H
191	PhCH2O	CF3	H	Cl
192	t-Bu	H	H	H
193	MeS	H	H	H
194	n-C5H11	H	H	H
195	n-C7H15	H	H	H
196	i-Pr	i-PrO	H	H
197	i-Pr	i-PrO	H	Cl
198	i-Pr	i-Pr	H	Cl
199	Cl	Cl	H	Cl
200	PhCH2S	H	H	H
201	PhCH2S	H	H	Cl
202	Et	H	H	H
203	i-Bu	H	H	H
204	PhOCH2—	H	H	H

[0094] 4-(3,5-dichlorophenoxy)benzyl bromide

[0095] Using 3,5-dichlorophenol and 4-fluorobenzaldehyde, reactions were carried out in the same manner as in Reference Example 1 to obtain 4-(3,5-dichlorophenoxy)benzaldehyde. The subsequent reactions were carried out in the same manner as in Reference Example 123, except that sodium borohydride was used in place of lithium aluminum hydride. This gave 4-(3,5-dichlorophenoxy)benzyl alcohol. The alcohol (2.03 g) and carbon tetrabromide (2.75 g) in methylene chloride (30 mL) were stirred at 0° C., and triphenyl phosphine (2.17 g) was added to the solution. The resulting mixture was stirred for 1 hour at 0° C. and then for 30 minutes at room temperature. Subsequently, the solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=20:1). In this manner, the desired product (3.12 g) was obtained as a colorless oil.

[0096] 4′-benzyloxyphenethyl iodide

[0097] Using ethyl 4′-(benzyloxy)phenyl acetate as a starting material, reactions were carried out in the same manner as in Reference Example 123 to obtain 4′-benzyloxyphenethyl alcohol. Using the alcohol, reactions were then carried out in the same manner as in Reference Example 164 to obtain the desired product as a pale yellow oil.

[0098] 4′-benzyloxy=dihydrocinnamyl iodide

[0099] Using 4′-benzyloxydihydrocinnamyl alcohol, reactions were carried out in the same manner as in Reference Example 164 to obtain the desired product as a yellow powder.

[0100] 1-benzyloxy-4-iodobutylbenzene

[0101] Using methyl 4-(4-benzyloxyphenyl)butyrate as a starting material, reactions were carried out in the same manner as in Reference Example 206 to obtain the desired product as a colorless oil.

[0102] 1-iodopropyl-4-[(3-methanesulfinyl)phenoxy]benzene

[0103] The compound of Reference Example 193 (1.80 g) was dissolved in methylene chloride (30 mL). While the solution was stirred at 0° C., m-chloroperbenzoic acid (770 mg) was added in small portions. With the temperature maintained, the mixture was stirred for 24 hours at room temperature and water was added to the mixture. The resulting mixture was then extracted with ethyl acetate. The organic phase was sequentially washed with a saturated aqueous solution of sodium carbonate and a saturated aqueous solution of sodium chloride and was then dried with anhydrous sodium sulfate. Subsequently, the solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=2:1 and then 1:2). In this manner, the desired product (1.29 g) was obtained as a yellow oil.

[0104] 4′-[(3,5-bistrifluoromethyl)phenoxy]cinnamyl chloride

[0105] Ethyl 4′-[(3,5-bistrifluoromethyl)phenoxy]cinnamate (500 mg) was dissolved in THF (20 mL). While the solution was stirred at 0° C., a 1 mol/L diisobutylaluminum hydride-toluene solution (3.0 mL) was added. With the temperature maintained, the solution was stirred for 1.5 hours, and a 2 mol/L aqueous solution of sodium hydroxide was added to the solution. The resulting mixture was then extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was then dried with anhydrous sodium sulfate. Subsequently, the solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=3:1). This gave an alcohol (377 mg) as a colorless oil. The alcohol so obtained (296 mg) was dissolved in DMF (5 mL), and lithium chloride (35.0 mg), collidine (0.120 mL), and methanesulfonyl chloride (0.070 mL) were added to the solution at 0° C. With the temperature maintained, the mixture was stirred for 1 hour. Subsequently, the reaction mixture was decanted into water and was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=20:1). In this manner, the desired product (241 mg) was obtained as a colorless powder.

[0106] The compounds were synthesized in the same manner as in Reference Example 1.

[0107]

TABLE 6
Reference
Example	R1	R2	R3	R4
211	Ph(CH2)2	c-CF3	H	Cl
212	PhCH2O	c-H	H	Me
213	PhCH2O	c-H	H	Et
214	PhCH2O	c-H	H	SMe
215	PhO	c-H	H	Cl
216	CF3	a-Cl	H	H
217	CF3	b-Cl	H	H
218	CF3	d-Cl	H	H
219	CF3	c-NO2	H	H

[0108] 2-fluoro-4-[(3-benzyloxy)phenoxy]benzaldehyde

[0109] Using 3-benzyloxyphenyboric acid and 2-fluoro-4-hydroxybenzaldehyde, the desired product was obtained as a colorless oil in the same manner as in Reference Example 38.

[0110] Using the compounds of Reference Examples 211 though 220, the compounds were synthesized in the same manner as in Reference Example 39.

[0111]

TABLE 7
Reference
Example	R1	R2	R3	R4
221	Ph(CH2)2	c-CF3	H	Cl
222	PhCH2O	c-H	H	Me
223	PhCH2O	c-H	H	Et
224	PhCH2O	c-H	H	SMe
225	PhO	c-H	H	Cl
226	CF3	a-Cl	H	H
227	CF3	b-Cl	H	H
228	CF3	d-Cl	H	H
229	CF3	c-NO2	H	H
230	PhCH2O	c-H	H	F

[0112] Using the compounds of Reference Examples 221 though 228 and 230, the compounds were synthesized in the same manner as in Reference Examples 80 through 83.

[0113]

TABLE 8
Reference
Example	R1	R2	R3	R4
231	Ph(CH2)2	c-CF3	H	Cl
232	PhCH2O	c-H	H	Me
233	PhCH2O	c-H	H	Et
234	PhCH2O	c-H	H	SMe
235	PhO	c-H	H	Cl
236	CF3	a-Cl	H	H
237	CF3	b-Cl	H	H
238	CF3	d-Cl	H	H
239	PhCH2O	c-H	H	F

[0114] Ethyl 4′-[3-chloro-5-(trifluoromethyl)phenoxy]dihydrocinnamate

[0115] Using the compound of Reference Example 229, reactions were carried out in the same manner as in Reference Example 81 to obtain ethyl 4′-[3-amino-5-(trifluoromethyl)phenoxy]dihydrocinnamate. A MeCN solution (15 mL) of this compound (1.27 g) was added to a MeCN solution (40 mL) of copper chloride (725 mg) and tBuONO (0.51 mL). The mixture was then stirred for 3 hours at room temperature, and water was added to the mixture. The resulting mixture was extracted with ethyl acetate. The organic phase was then washed with water and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=20:1). In this manner, the desired product (1.10 g) was obtained as a pale yellow oil.

[0116] Using the compounds of Reference Examples 231 through 240, the compounds were synthesized in the same manner as in Reference Example 123.

[0117]

TABLE 9
Reference
Example	R1	R2	R3	R4
241	Ph(CH2)2	c-CF3	H	Cl
242	PhCH2O	c-H	H	Me
243	PhCH2O	c-H	H	Et
244	PhCH2O	c-H	H	SMe
245	PhO	c-H	H	Cl
246	CF3	a-Cl	H	H
247	CF3	b-Cl	H	H
248	CF3	d-Cl	H	H
249	CF3	c-Cl	H	H
250	PhCH2O	c-H	H	F

[0118] Using the compounds of Reference Examples 241 through 250, the compounds were synthesized in the same manner as in Reference Example 164.

[0119]

TABLE 10
Reference
Example	R1	R2	R3	R4
251	Ph(CH2)2	c-CF3	H	Cl
252	PhCH2O	c-H	H	Me
253	PhCH2O	c-H	H	Et
254	PhCH2O	c-H	H	SMe
255	PhO	c-H	H	Cl
256	CF3	a-Cl	H	H
257	CF3	b-Cl	H	H
258	CF3	d-Cl	H	H
259	CF3	c-Cl	H	H
260	PhCH2O	c-H	H	F

[0120] 4′-[(3-benzyloxy)phenoxy]-2′-chlorophenethyl iodide

[0121] 4′-[(3-benzyloxy)phenoxy]-2′-chlorobenzyl cyanide

[0122] Using the compound of Reference Example 1, reactions were carried out in the same manner as in Reference Example 205 to obtain 4-[(3-benzyloxy)phenoxy]-2-chlorobenzyl bromide as a colorless oil. A DMSO solution (10 mL) of the bromide (1.38 g) was added dropwise to a solution (2 mL water and 5 mL DMSO) of KCN (245 mg) at 90° C., and the mixture was stirred for 10 minutes and then for another 30 minutes at room temperature. Subsequently, ice water was added to the mixture and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=5:1). In this manner, the desired product (1.02 g) was obtained as a colorless oil.

[0123] Ethyl 4′-[(3-benzyloxy)phenoxy]-2′-chlorophenylacetate

[0124] A solution (30 mL) of the compound of Reference Example 261-1 (1.02 g) and potassium hydroxide (819 mg) in a mixed solvent of water (2 mL) and ethanol (30 mL) was refluxed for 12 hours. The solution was made acidic by the addition of hydrochloric acid and was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was concentrated under reduced pressure and the resulting concentrate was dissolved in ethanol (10 mL) and thionyl chloride (11.0 mL) was added to the solution. The mixture was subsequently stirred for 1 hour at room temperature. The solvent was removed by distillation and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=10:1). In this manner, the desired product (1.01 g) was obtained as a colorless oil.

4′-[(3-benzyloxy)phenoxy]-2′-chlorophenethyl iodide

[0125] Using the compound of Reference Example 251-2, reactions were carried out in the same manner as in Reference Example 123 to obtain an alcohol. Then, using this alcohol, subsequent reactions are carried out in the same manner as in Reference Example 164 to obtain the desired product as a yellow oil.

[0126] 4-[(3-benzyloxy)phenoxy]-2-chloro-1-iodobutylbenzene

[0127] Using the compound of Reference Example 164, reactions were carried out in the same manner as in Reference Example 261 to obtain the desired product as a pale yellow oil.

[0128] Ethyl 5-[4-(3-benzyloxyphenoxy)-2-chlorophenyl]-2-t-butoxycarbonylamino-2-ethoxycarbonylpentanoate

[0129] Under argon, sodium=t-butoxide (1.40 g) was added, at room temperature, to a solution of diethyl 2-t-butoxycarbonylaminomalonate (3.60 mL) in a mixed solvent of THF (130 mL) and DMF (20 mL). The resulting mixture was stirred for 30 minutes at 80° C. The temperature was decreased down to room temperature and a THF solution (20 mL) of the compound of Reference Example 164 (6.22 g) was added dropwise. Subsequently, the mixture was refluxed for 5 hours and was decanted into ice water. The resulting mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure, and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=4:1). In this manner, the desired product (6.84 g) was obtained as a colorless oil.

[0130] FABMS: 626 ([M+H]+)

[0131] ¹H-NMR(400 MHz, CDCl₃) δ 1.22-1.30(6H, m), 1.42(9H, s), 1.57(2H, br s), 2.37(2H, br), 2.70(2H, t, J=7.8 Hz), 4.19-4.29(4H, m), 5.03(2H, s), 5.95(1H, bs), 6.57-6.62(2H, m), 6.74(1H, dd, J=8.3, 2.4 Hz), 6.83(1H, dd, J=8.3, 2.4 Hz), 6.98(1H, d, J=2.4 Hz), 7.13(1H, d, J=8.3 Hz), 7.23(1H, t, J=8.3 Hz), 7.33-7.43(5H, m)

[0132] Using the compounds of Reference Examples 165 through 204 and 209, reactions were carried out in the same manner as in Example 1 above to obtain the compounds shown in Table 11 below.

[0133]

TABLE 11
Ex-
am-					Charac-	Yield
ple	R1	R2	R3	R4	teristics	(%)
2	CF3	H	H	H	Colorless oil	100
3	CF3	H	MeO	H	Colorless oil	100
4	CF3	H	H	MeO	Colorless oil	100
5	CF3	H	Cl	H	Colorless oil	100
6	CF3	H	H	Cl	Colorless oil	100
7	CF3	H	H	PhCH2O	Colorless oil	100
8	CF3	H	CF3	H	Colorless oil	100
9	CF3	H	H	CF3	Colorless oil	92
10	CF3	CF3	H	Cl	Colorless oil	89
11	CF3	Ph(CH2)2	H	H	Colorless oil	97
12	CF3	H	H	F	Colorless oil	100
13	Ph(CH2)2	Ph(CH2)2	H	H	Colorless oil	95
14	Ph(CH2)2	H	H	Cl	Colorless oil	83
15	Ph(CH2)2	H	H	CF3	Colorless oil	90
16	Ph(CH2)2	Ph(CH2)2	H	Cl	Colorless oil	98
17	Ph(CH2)2	Ph(CH2)2	H	CF3	Colorless oil	100
18	PhCH2O	H	H	H	Colorless oil	95
19	PhCH2O	PhCH2O	H	H	Colorless oil	—
20	PhCH2O	PhCH2O	H	Cl	Colorless oil	—
21	PhCH2O	Cl	H	Cl	Colorless oil	100
22	PhCH2O	H	H	Br	Colorless oil	100
23	PhCH2O	H	H	CF3	Colorless oil	100
24	PhCH2O	H	H	Ph	Colorless oil	—
25	PhCH2O	CF3	H	H	Colorless oil	99
26	PhCH2O	CF3	H	Cl	Colorless oil	91
27	t-Bu	H	H	H	Colorless oil	64
28	MeS	H	H	H	Colorless oil	83
29	n-C5H11	H	H	H	Colorless oil	86
30	n-C7H15	H	H	H	Colorless oil	88
31	i-Pr	i-PrO	H	H	Colorless oil	95
32	i-Pr	i-PrO	H	Cl	Colorless oil	100
33	i-Pr	i-Pr	H	Cl	Colorless oil	66
34	Cl	Cl	H	Cl	Colorless oil	74
35	PhCH2S	H	H	H	Colorless oil	—
36	PhCH2S	H	H	Cl	Colorless oil	—
37	Et	H	H	H	Colorless oil	100
38	i-Bu	H	H	H	Colorless oil	76
39	MeSO	H	H	H	Colorless oil	100
40	t-BuMe2SiO	H	H	Cl	Colorless oil	82
41	PhOCH2	H	H	H	Colorless oil	100
42	PhCH2O	H	H	i-Pr	Colorless oil	—
The mark “—” means yield is shown in Table 12 as a total yield.

[0134] Ethyl 2-t-butoxycarbonylamino-2-ethoxycarbonyl-3-[4-(3,5-dichlorophenoxy)phenyl]propionate

[0135] Using the compound of Reference Example 205, reactions were carried out in the same manner as in Example 1 to obtain the desired product as a colorless oil.

[0136] ¹H-NMR(400 MHz, CDCl₃) δ 1.28(6H, t, J=7.3 Hz), 1.47(9H, br s), 3.62(2H, br s), 4.19-4.31(4H, m), 5.79(1H, br s), 6.85(2H, d, J=2.0 Hz), 6.92(2H, d, J=8.8 Hz), 7.04-7.08(3H, m)

[0137] Ethyl 4-[(4-benzyloxy)phenyl]-2-t-butoxycarbonylamino-2-ethoxycarbonylbutyrate

[0138] Using the compound of Reference Example 206, reactions were carried out in the same manner as in Example 1 to obtain the desired product as a colorless oil.

[0139] ¹H-NMR(400 MHz, CDCl₃) δ 1.23(6H, t, J=7.3 Hz), 1.44(9H, s), 2.44-2.48(2H,m), 2.60(2H, br s), 4.13-4.31(4H, m), 5.04(2H, s), 5.99(1H, br s), 6.88(2H, d, J=8.8 Hz), 7.07(2H, d, J=8.3 Hz), 7.29-7.44(5H, m)

[0140] Ethyl 5-[(4-benzyloxy)phenyl]-2-t-butoxycarbonylamino-2-ethoxycarbonylpentanoate

[0141] Using the compound of Reference Example 207, reactions were carried out in the same manner as in Example 1 to obtain the desired product as a light yellow oil.

[0142] ¹H-NMR(400 MHz, CDCl₃) δ 1.22(6H, t, J=7.1 Hz), 1.42(9H, s), 1.44-1.47(2H,m), 2.31(2H, br s), 2.57(2H, t, J=7.6 Hz), 4.11-4.27(4H, m), 5.03(2H, s), 5.92(1H, br s), 6.88(2H, d, J=8.8 Hz), 7.06(2H, d, J=8.8 Hz), 7.29-7.43(5H, m)

[0143] Ethyl 6-[(4-benzyloxy)phenyl]-2-t-butoxycarbonylamino-2-ethoxycarbonylhexanoate

[0144] Using the compound of Reference Example 208, reactions were carried out in the same manner as in Example 1 to obtain the desired product as a colorless oil.

[0145] ¹H-NMR(400 MHz, CDCl₃) δ 1.16-1.24(2H, m), 1.23(6H, t, J=7.1 Hz), 1.42(9H, s), 1.56-1.63(2H, m), 2.30(2H, br), 2.54(2H, t, J=7.8 Hz), 4:16-4.29(4H, m), 5.03(2H, s), 5.92(1H, br s), 6.88(2H, d, J=8.3 Hz), 7.06(2H, d, J=8.3 Hz), 7.32-7.44(5H, m)

[0146] Ethyl 5-[4-(3,5-bistrifluoromethylphenoxy)phenyl]-2-t-butoxycarbonylamino-2-ethoxycarbonyl-4-pentenoate

[0147] Using the compound of Reference Example 210, reactions were carried out in the same manner as in Example 1 to obtain the desired product as a colorless oil.

[0148] ¹H-NMR(400 MHz, CDCl₃) δ 1.27(6H, t, J=7.0 Hz), 1.44(9H, s), 3.20(2H, d, J=7.0 Hz), 4.20-4.32(4H, m), 5.97(1H, br s), 6.02(1H, dt, J=15.9, 7.0 Hz), 6.45(1H, d, J=15.9 Hz), 6.98(2H, d, J=8.5 Hz), 7.36(2H, d, J=8.5 Hz), 7.38(2H, s), 7.57(1H, s)

[0149] Ethyl 2-t-butoxycarbonylamino-2-ethoxycarbonyl-5-[4-(3-isopropoxyphenoxy)phenyl]pentanoate

[0150] The compound of Example 18 was reduced by catalytic reduction as in Reference Example 81. The resultant phenol (850 mg) was dissolved in DMF (20 mL), and 2-iodopropane (0.2 mL) and potassium carbonate (500 mg) were added to the solution. The mixture was then stirred for 4 hours at 60° C. Subsequently, water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=4:1). In this manner, the desired product (760 mg) was obtained as a colorless oil.

[0151] ¹H-NMR(400 MHz, CDCl₃) δ 1.23(6H, t, J=7.3 Hz), 1.31(6H, d, J=5.9 Hz), 1.42(9H, s), 1.45-1.52(2H, m), 2.34(2H, br), 2.61(2H, t, J=7.8 Hz), 4.17-4.27(4H, m), 4.50(1H, heptet, J=5.9 Hz), 5.94(1H, br s), 6.50-6.53(2H, m), 6.59-6.62(1H, m), 6.92(2H, d, J=8.8 Hz), 7.10(2H, d, J=8.8 Hz), 7.18(1H, t, J=8.8 Hz)

[0152] Ethyl 2-t-butoxycarbonylamino-2-ethoxycarbonyl-5-[4-(3-methanesulfonylphenoxy)phenyl]pentanoate

[0153] The compound of Example 28 (1.00 g) was dissolved in methylene chloride (30 mL) and m-chloroperbenzoic acid (610 mg) was added to the solution. The mixture was then stirred for 6 hours at room temperature. Subsequently, water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=1:1). In this manner, the desired product (610 mg) was obtained as a colorless oil.

[0154] ¹H-NMR(400 MHz, CDCl₃) δ 1.24(6H, t, J=7.3 Hz), 1.42(9H, s), 1.47-1.56(2H, m), 2.34(2H, br), 2.64(2H, t, J=7.8 Hz), 3.04(3H, s), 4.18-4.26(4H, m), 5.95(1H, br), 6.95(2H, d, J=8.8 Hz), 7.17(2H, t, J=8.8 Hz), 7.20-7.30(3H,m), 7.47-7.52(2H, m), 7.62(1H, d, J=8.8 Hz)

[0155] Ethyl 5-[4-(3,5-bistrifluoromethylphenoxy)phenyl]-2-t-butoxycarbonylamino-2-ethoxycarbonylpentanoate

[0156] The compound of Example 44 was reduced by catalytic reduction as in Reference Example 81. The resultant phenol was reacted with 3,5-bis(trifluoromethyl)phenylboric acid in the same manner as in Reference Example 38 to obtain the desired product as a pale yellow oil.

[0157] ¹H-NMR(400 MHz, CDCl₃) δ 1.24(6H, t, J=7.3 Hz), 1.43(9H, s), 1.47-1.58(4H, m), 2.36(2H, br s), 2.66(2H, t, J=7.3 Hz), 4.18-4.26(4H, m), 5.96(1H, br s), 6.96(2H, d, J=8.3 Hz), 7.20(2H, d, J=8.3 Hz), 7.36(2H, s), 7.55(1H, s)

[0158] 2-[4-(3-benzyloxyphenoxy)-2-chlorophenyl]propyl-2-t-butoxycarbonylamino-1,3-propanediol

[0159] The compound of Example 1 (6.84 g) was dissolved in THF (150 mL). While the solution was stirred at 0° C., lithium borohydride (960 mg) was added to the solution. Ethanol (10 mL) was then added to the mixture and the mixture was stirred for 8 hours as the temperature was gradually increased to room temperature. Subsequently, ice water was added to the mixture and the organic solvent was removed by distillation under reduced pressure. A 10% aqueous solution of citric acid was added to the residue to adjust the pH to 3, and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure. The residue was purified by silica gel column chromatography (hexane:ethyl acetate=1:1) to obtain the desired product (3.50 g) as a colorless viscous oil.

[0160] FABMS: 542([M+H]+)

[0161] ¹H-NMR(400 MHz, CDCl₃) δ 1.43(9H, s), 1.66(4H, br s), 2.69(2H, t, J=6.8 Hz), 3.40(2H, br), 3.60(2H, dd, J=11.3, 5.9 Hz), 3.84(2H, dd, J=11.3, 3.8 Hz), 4.92(1H, br s), 5.03(2H, s), 6.59-6.62(2H, m), 6.75(1H, dd, J=8.3, 2.5 Hz), 6.84(1H, dd, J=8.3, 2.5 Hz), 7.00(1H, d, J=2.5 Hz), 7.14(1H, d, J=8.3 Hz), 7.24(1H, t, J=8.3 Hz), 7.31-7.43(5H, m)

[0162] Using the compounds of Examples 2 through 42 and 48 through 50, reactions were carried out in the same manner as in Example 51 above to synthesize the compounds shown in Table 12 below.

[0163]

TABLE 12
Example	R1	R2	R3	R4	Characteristics	Yield (%)
52	CF3	H	H	H	Colorless oil	71
53	CF3	H	MeO	H	Colorless oil	76
54	CF3	H	H	MeO	Colorless oil	45
55	CF3	H	Cl	H	Colorless oil	58
56	CF3	H	H	Cl	Colorless oil	68
57	CF3	H	H	PhCH2O	Colorless oil	64
58	CF3	H	CF3	H	Colorless oil	68
59	CF3	H	H	CF3	Colorless oil	41
60	CF3	CF3	H	Cl	Colorless oil	77
61	CF3	Ph(CH2)2	H	H	Colorless oil	80
62	CF3	H	H	F	Colorless oil	63
63	Ph(CH2)2	Ph(CH2)2	H	H	Colorless oil	71
64	Ph(CH2)2	H	H	Cl	Colorless oil	84
65	Ph(CH2)2	H	H	CF3	Colorless oil	72
66	Ph(CH2)2	Ph(CH2)2	H	Cl	Colorless oil	61
67	Ph(CH2)2	Ph(CH2)2	H	CF3	Colorless oil	54
68	PhCH2O	H	H	H	Colorless oil	76
69	PhCH2O	PhCH2O	H	H	Colorless oil	(45)
70	PhCH2O	PhCH2O	H	Cl	Colorless oil	(17)
71	PhCH2O	Cl	H	Cl	Colorless oil	61
72	PhCH2O	H	H	Br	Colorless oil	61
73	PhCH2O	H	H	CF3	Colorless oil	83
74	PhCH2O	H	H	Ph	Colorless oil	(50)
75	PhCH2O	CF3	H	H	Colorless oil	83
76	PhCH2O	CF3	H	Cl	Colorless oil	67
77	t-Bu	H	H	H	Colorless oil	78
78	MeS	H	H	H	Colorless powder	56
79	n-C5H11	H	H	H	Colorless oil	98
80	n-C7H15	H	H	H	Colorless Oil	90
81	i-Pr	i-PrO	H	H	Colorless oil	72
82	i-Pr	i-PrO	H	Cl	Colorless oil	82
83	i-Pr	i-Pr	H	Cl	Colorless oil	33
84	Cl	Cl	H	Cl	Colorless oil	79
85	PhCH2S	H	H	H	Colorless oil	(20)
86	PhCH2S	H	H	Cl	Colorless oil	(11)
87	Et	H	H	H	Colorless oil	76
88	i-Bu	H	H	H	Colorless oil	92
89	MeSO	H	H	H	Colorless oil	67
90	MeSO2	H	H	H	Colorless amorphous	78
91	i-PrO	H	H	H	Colorless oil	89
92	tBuMe2SiO	H	H	Cl	Colorless oil	68
93	CF3	CF3	H	H	Colorless oil	72
94	PhOCH2	H	H	H	Colorless oil	64
95	PhCH2O	H	H	i-Pr	Colorless oil	(62)
In the parentheses, shown is the total, yield of the two steps.

[0164] 2-t-butoxycarbonylamino-2-[4-(3,5-dichlorophenoxy)benzyl]-1,3-propanediol

[0165] Using the compound of Example 43, reactions were carried out in the same manner as in Example 51 to obtain the desired product as a colorless amorphous.

[0166] ¹H-NMR(400 MHz, CDCl₃) δ 1.46(9H, s), 2.94(2H, s), 3.60(2H, d, J=11.7 Hz), 3.75(2H, d, J=11.7 Hz), 4.93(1H, br s), 6.87(2H, d, J=2.0 Hz), 6.98(2H, d, J=8.8 Hz), 7.08(1H, t, J=2.0 Hz), 7.26(2H, d, J=8.8 Hz)

[0167] 2-(4-benzyloxyphenyl)ethyl-2-t-butoxycarbonylamino-1,3-propanediol

[0168] Using the compound of Example 44, reactions were carried out in the same manner as in Example 51 to obtain the desired product as a colorless powder.

[0169] ¹H-NMR(400 MHz, CDCl₃) δ 1.45(9H, s), 1.83-1.88(2H, m), 2.54-2.59(2H, m), 3.39(2H, br s), 3.64(2H, d, J=11.2 Hz), 3.88(2H, d, J=11.2 Hz), 5.01(1H,br s), 5.03(2H, s), 6.90(2H, d, J=8.3 Hz), 7.10(2H, d, J=8.3 Hz), 7.30-7.44(5H, m)

[0170] 2-[(4-benzyloxy)phenyl]propyl-2-t-butoxycarbonylamino-1,3-propanediol

[0171] Using the compound of Example 45, reactions were carried out in the same manner as in Example 51 to obtain the desired product as a pale yellow oil.

[0172] ¹H-NMR(400 MHz, CDCl₃) δ 1.43(9H, s), 1.50-1.70(4H, m), 2.52-2.57(2H, m), 3.57(2H, d, J=11.2 Hz), 3.82(2H, d, J=11.2 Hz), 4.86(1H, br s), 5.04(2H,s), 6.90(2H, d, J=8.8 Hz), 7.08(2H, d, J=8.8 Hz), 7.31-7.44(5H, m)

[0173] 2-[(4-benzyloxy)phenyl]butyl-2-t-butoxycarbonylamino-1,3-propanediol

[0174] Using the compound of Example 46, reactions were carried out in the same manner as in Example 51 to obtain the desired product as a colorless oil.

[0175] ¹H-NMR(400 MHz, CDCl₃) δ 1.27-1.35(2H, m), 1.43(9H, s), 1.54-1.63(4H, m), 2.56(2H, t, J=7.6 Hz), 3.41(2H, br s), 3.58(2H, d, J=11.7 Hz), 3.82(2H, d, J=11.7 Hz), 4.88(1H, br s), 5.04(2H, s), 6.89(2H, d, J=8.8 Hz), 7.07(2H, d, J=8.8 Hz), 7.33-7.43(5H, m)

[0176] 2-[4′-(3,5-bistrifluoromethylphenoxy)cinnamyl]-2-t-butoxycarbonylamino-1,3-propanediol

[0177] Using the compound of Example 47, reactions were carried out in the same manner as in Example 51 to obtain the desired product as a colorless amorphous.

[0178] ¹H-NMR(400 MHz, CDCl₃) δ 1.44(9H, s), 2.55(2H, d, J=7.8 Hz), 3.65(2H, d, J=11.2 Hz), 3.78(2H, br), 3.85(2H, d, J=11.2 Hz), 5.12(1H, s), 6.20(1H, dt, J=16.1, 7.8 Hz), 6.51(1H, d, J=16.1 Hz), 7.01(2H, d, J=8.3 Hz) 7.38(2H,s), 7.39(2H, d, J=8.3 Hz), 7.57(1H, s)

[0179] 5-[(4-benzyloxy)phenyl]propyl-5-t-butoxycarbonylamino-2,2-di-t-butyl-1,3,2-dioxasilane

[0180] At 0° C., a methylene chloride solution (5 mL) of di-t-butylsilyl bistrifluoromethanesulfonate (1.67 g) was added to a DMF solution (30 mL) of the compound of Example 98 (1.50 g) and 2,6-lutidine (0.841 mL). With the temperature maintained, the mixture was stirred for 1 hour. Subsequently, the mixture was decanted into ice water and was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure. The residue was purified by silica gel column chromatography (hexane:ethyl acetate=20:1) to obtain the desired product (1.67 g) as a colorless powder.

[0181] ¹H-NMR(400 MHz, CDCl₃) δ 1.04(9H, s), 1.06(9H, s), 1.42(9H, s), 1.46-1.56(4H, br s), 2.51(2H, t, J=6.8 Hz), 3.88(2H, d, J=11.2 Hz), 4.22(2H, d, J=11.2 Hz), 4.90(1H, br s), 5.04(2H, s), 6.89(2H, d, J=8.3 Hz), 7.06(2H, d, J=8.3 Hz), 7.32-7.44(5H, m)

[0182] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-(4-hydroxyphenyl)propyl-1,3,2-dioxasilane

[0183] Using the compound of Example 101, catalytic reduction was carried out in the same manner as in Reference Example 81 to obtain the desired product as a pale brown amorphous.

[0184] ¹H-NMR(400 MHz, CDCl₃) δ 1.04(9H, s), 1.06(9H, s), 1.43(9H, s), 1.47-1.61(4H, m), 2.49(2H, t, J=6.8 Hz), 3.88(2H, d, J=11.3 Hz), 4.22(2H, d, J=11.3 Hz), 4.88(1H, br s), 4.91(1H, br s), 6.74(2H, d, J=8.3 Hz), 6.99(2H, d, J=8.3 Hz)

[0185] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-(4-hydroxyphenyl)ethyl-1,3,2-dioxasilane

[0186] Using the compound of Example 97, reactions were carried out in the same manner as in Examples 101 and 102 to obtain the desired product as a colorless powder.

[0187] ¹H-NMR(400 MHz, CDCl₃) δ 1.06(9H, s), 1.07(9H, s), 1.46(9H, s), 1.79(2H, m), 2.44-2.50(2H, m), 3.93(2H, d, J=11.2 Hz), 4.26(2H, d, J=11.2 Hz), 4.92(1H, br s), 5.01(1H, br s), 6.73(2H, d, J=8.3 Hz), 7.01(2H, d, J=8.3 Hz)

[0188] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-(4-hydroxyphenyl)butyl-1,3,2-dioxasilane

[0189] Using the compound of Example 99, reactions were carried out in the same manner as in Examples 101 and 102 to obtain the desired product as a colorless amorphous.

[0190] ¹H-NMR(400 MHz, CDCl₃) δ 1.05(9H, s), 1.07(9H, s), 1.20-1.30(2H, m), 1.42(9H, s), 1.50-1.60(4H, m), 2.51(2H, t, J=7.6 Hz), 3.89(2H, d, J=11.2 Hz), 4.22(2H, d, J=11.2 Hz), 4.78(1H, br s), 4.91(1H, br s), 6.73(2H, d, J=8.3 Hz), 7.00(2H, d, J=8.3 Hz)

[0191] 5-t-butoxycarbonylamino-5-[4-(3-hydroxyphenoxy)phenyl]propyl-2,2-dimethyl-1,3-dioxane

[0192] 2,2-dimethoxypropane (7.4 mL) and paratoluenesulfonic acid (100 mg) were added to a DMF solution (30 mL) of the compound of Example 68 (3.00 g). The mixture was stirred for 6 hours while heated to 80° C. Subsequently, the mixture was decanted into water and was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure. The residue was purified by silica gel column chromatography (hexane:ethyl acetate=3:1) to obtain the acetonide (2.68 g) as a colorless powder. The resultant acetonide was reduced by catalytic reduction as in Reference Example 81 to obtain the desired product (2.23 g) as a colorless powder.

[0193] ¹H-NMR(400 MHz, CDCl₃) δ 1.40(3H, s), 1.42(12H, s), 1.54-1.69(4H, m), 2.61(2H, t, J=7.8 Hz), 3.63(2H, d, J=11.2 Hz), 3.87(2H, d, J=11.2 Hz), 4.86(1H,br), 5.29(1H, br s), 6.32(1H, br s), 6.52(1H, dd, J=8.3, 2.4 Hz), 6.57(1H, dd, J=8.3, 2.4 Hz), 6.95(2H, d, J=8.3 Hz), 7.13(2H, d, J=8.3 Hz), 7.16(1H, t, J=8.3 Hz)

[0194] 5-t-butoxycarbonylamino-5-[2-chloro-4-(3-hydroxyphenoxy)phenyl]propyl-2,2-dimethyl-1,3-dioxane

[0195] Using the compound of Example 51, reactions were carried out in the same manner as in Example 105 to obtain the desired product as a colorless powder.

[0196] Alternatively, an acetonide (3.21 g) obtained by using the compound of Example 92 was dissolved in THF (100 mL). While the solution was stirred at 0° C., a 1 mol/L tetrabutylammoniumfluoride-THF solution (10 mL) was added dropwise. After 10 minutes, water was added to the mixture and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure to obtain the desired product (2.60 g).

[0197] FABMS: 492 ([M+H]+)

[0198] ¹H-NMR(400 MHz, CDCl₃) δ 1.41(3H, s), 1.42(12H, s), 1.55-1.73(4H, m), 2.70(2H, t, J=7.3 Hz), 3.66(2H, d, J=11.7 Hz), 3.88(2H, d, J=11.7 Hz), 4.89(1H,br), 5.97(1H, br), 6.40(1H, br s), 6.56(1H, dd, J=8.3, 2.4 Hz), 6.62(1H, dd, J=8.3, 2.4 Hz), 6.86(1H, dd, J=8.3, 2.4 Hz), 7.01(1H, d, J=2.4 Hz), 7.14(1H, d, J=8.3 Hz), 7.18(1H, d, J=8.3 Hz)

[0199] 5-t-butoxycarbonylamino-5-[2-chloro-4-(3-(3,5-dichlorobenzyloxy)phenoxy)phenyl]propyl-2,2-dimethyl-1,3-dioxane

[0200] Diethyl azodicarboxylate (0.31 mL) was added to a THF solution (5 mL) containing the compound of Example 106 (650 mg), 3,5-dichlorobenzyl alcohol (350 mg), triphenylphosphine (530 mg). The mixture was stirred for 18 hours. Subsequently, water was added to the mixture and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure. The residue was purified by silica gel column chromatography (hexane:ethyl acetate=3:1) to obtain the desired product (440 mg) as a colorless powder.

[0201] ¹H-NMR(400 MHz, CDCl₃) δ 1.41(3H, s), 1.42(3H, s), 1.43(9H, s), 1.54-1.60(2H, m), 1.75(2H, br), 2.69(2H, t, J=7.3 Hz), 3.66(2H, d, J=11.7 Hz), 3.88(2H, d, J=11.7 Hz), 4.89(1H, br), 4.98(2H, s), 6.58-6.64(2H, m), 6.70(1H, dd, J=8.3, 2.4 Hz), 6.84(1H, dd, J=8.3, 2.4 Hz), 7.00(1H, d, J=2.4 Hz), 7.15(1H, d, J=8.3 Hz), 7.22-7.32(4H, m)

[0202] 5-t-butoxycarbonylamino-2,2-dimethyl-5-[4-(3-phenoxy)phenoxyphenyl]propyl-1,3-dioxane

[0203] The compound of Example 105 was reacted with phenylboric acid in the same manner as in Reference Example 38 to obtain the desired product as a colorless powder.

[0204] ¹H-NMR(400 MHz, CDCl₃) δ 1.40(3H, s), 1.42(3H, s), 1.43(9H, s), 1.54-1.61(2H, m), 1.70(2H, br), 2.58(2H, t, J=7.3 Hz), 3.64(2H, d, J=11.2 Hz), 3.89(2H, d, J=11.2 Hz), 4.87(1H,br), 6.66-6.71(3H, m), 6.94(2H, d, J=8.3 Hz), 7.02(2H, d, J=8.3 Hz), 7.11-7.13(3H, m), 7.21(1H, t, J=8.3 Hz), 7.34(2H, t, J=8.3 Hz)

[0205] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-[4-(3-isopropylphenoxy)phenyl]propyl-1,3,2-dioxasilane

[0206] The compound of Example 102 (200 mg), 3-isopropylphenylboric acid (141 mg), anhydrous copper acetate (II) (97.4 mg), and molecular sieve powder-4A (400 mg) were suspended in dichloromethane (5 mL). Triethylamine (120 μL) was then added to the suspension and the suspension was stirred for 8 hours at room temperature. Subsequently, additional 3-isopropylphenylboric acid (141 mg) and triethylamine (120 μL) were added and the resulting mixture was further stirred overnight at room temperature. The reaction mixture was diluted with a mixture of hexane and ethyl acetate (hexane:ethyl acetate=2:1) and was filtered through celite to remove insoluble materials. The filtrate was concentrated under reduced pressure and the residue was purified by silica gel column chromatography (hexane:ethyl acetate=30:1) to obtain the desired product (188 mg) as a colorless oil.

[0207] ¹H-NMR(400 MHz, CDCl₃) δ 1.05(9H, s), 1.07(9H, s), 1.23(6H, d, J=6.8 Hz), 1.43(9H, s), 1.55(4H, br s), 2.55(2H, t, J=7.1 Hz), 2.84-2.91(1H, m), 3.89(2H, d, J=11.7 Hz), 4.23(2H, d, J=11.7 Hz), 4.91(1H, br s), 6.75-6.79(1H, m), 6.89-6.91(1H, m), 6.91(2H, d, J=8.8 Hz), 6.95(1H, d, J=7.8 Hz), 7.09(2H, d, J=8.8 Hz), 7.22(1H, t, J=7.8 Hz)

[0208] The compound of Example 102 was reacted with different phenylboric acids in the same manner as in Example 109 described above to synthesize the compounds shown in Table 13 below.

[0209]

TABLE 13
Example	R1	R2	Yield (%)	Characteristics
110	F	H	60	Colorless oil
111	Cl	H	61	Colorless oil
112	Br	H	59	Colorless oil
113	Me	H	84	Colorless oil
114	Ph	H	74	Colorless amorphous
115	MeO	H	69	Colorless oil
116	EtO	H	76	Colorless oil
117	CF3O	H	68	Colorless oil
118	CH2OH	H	41	Colorless powder
119	Ac	H	80	Pale yellow oil
120	NO2	H	—	Pale yellow powder
121	CN	H	44	Colorless oil
122	F	F	79	Colorless oil
123	Cl	Cl	60	Pale yellow oil
124	CF3	CF3	83	Colorless oil
125	CHO	H	74	Colorless oil
The mark “—” means yield is shown in Table 15 as a total yield.

[0210] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-[4-(3,5-bistrifluoromethylphenoxy)phenyl]ethyl-1,3,2-dioxasilane

[0211] The compound of Example 103 was reacted with 3,5-bis(trifluoromethyl)phenylboric acid in the same manner as in Example 109 to obtain the desired product as a colorless powder.

[0212] ¹H-NMR(400 MHz, CDCl₃) δ 1.07(9H, s), 1.09(9H, s), 1.47(9H, s), 1.87(2H, m), 2.55-2.60(2H, m), 3.97(2H, d, J=11.2 Hz), 4.28(2H, d, J=11.2 Hz), 5.05(1H, br s), 6.96(2H, d, J=8.3 Hz), 7.21(2H, d, J=8.3 Hz), 7.34(2H, s), 7.54(1H, s)

[0213] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-[4-(3,5-bistrifluoromethylphenoxy)phenyl]butyl-1,3,2-dioxasilane

[0214] The compound of Example 104 was reacted with 3,5-bis(trifluoromethyl)phenylboric acid in the same manner as in Example 109 to obtain the desired product as a colorless oil.

[0215] ¹H-NMR(400 MHz, CDCl₃) δ 1.05(9H, s), 1.08(9H, s), 1.25-1.31(2H, m), 1.42(9H, s), 1.55-1.63(4H, m), 2.61(2H, t, J=7.8 Hz), 3.91(2H, d, J=11.2 Hz), 4.23(2H, d, J=11.2 Hz), 4.92(1H, br s), 6.95(2H, d, J=8.3 Hz), 7.19(2H, d,J=8.3 Hz), 7.36(2H, s), 7.54(1H, s)

[0216] The compounds of Examples 103 and 104 were reacted with 3,5-dichlorophenylboric acid in the same manner as in Example 109 to obtain the following products:

[0217]

Example	n	Yield (%)	Characteristics
128	1	49	Colorless oil
129	3	67	Colorless oil

[0218] 5-t-butoxycarbonylamino-5-[4-(3-(4-chlorobenzyloxy)phenoxy)phenyl]propyl-2,2-dimethyl-1,3-dioxane

[0219] Potassium carbonate (150 mg) and p-chlorobenzyl bromide (103 mg) were added to a DMF solution (5 mL) of the compound of Example 105 (150 mg) and the mixture was stirred for 1 hour at 70° C. Subsequently, the mixture was decanted into water and was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure. The residue was purified by silica gel column chromatography (hexane:ethyl acetate=3:1) to obtain the desired product (170 mg) as a colorless powder.

[0220] ¹H-NMR (400 MHz, CDCl₃) δ 1.40(3H, s), 1.42(3H, s), 1.44(9H, s), 1.56-1.61(2H, m), 1.71(2H, br), 2.59(2H, t, J=7.3 Hz), 3.64(2H, d, J=11.7 Hz), 3.89(2H, d, J=11.7 Hz), 4.87(1H,br), 4.98(2H, s), 6.58-6.60(2H, m), 6.66-6.68(1H, m), 6.92(2H, d, J=8.3 Hz), 7.12(2H, d, J=8.3 Hz), 7.20 (1H, t, J=8.3 Hz), 7.34(4H, s)_o

[0221] The compounds of Example 105 and 106 were reacted with different alkylhalides in the same manner as in Example 130 described above to synthesize the compounds shown in Table 14 below:

[0222]

TABLE 14
Example	R	R′	Characteristics	Yield (%)
131		H	Colorless powder	100
132		H	Colorless powder	100
133		Cl	Coloreless powder	75
134		Cl	Colorless powder	94
135		Cl	Colorless powder	100
136		H	Colorless powder	85
137		H	Colorless amorphous	86
138		H	Pale yellow powder	100
139		H	Colorless powder	100
140		H	Colorless amorphous	76
141	Ph2CH	H	Colorless powder	58
142		H	Colorless oil	100
143		Cl	Colorless powder	84

[0223] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-[4-(3,5-bistrifluoromethylphenoxy)phenyloxy]ethyl-1,3,2-dioxasilane
a) 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-hydroxyethyl-1,3,2-dioxasilane

[0224] Using benzylbromoethylether and diethyl 2-t-butoxycarbonylaminomalonate, reactions were carried out in the same manner as in Example 1. Subsequently, the reaction processes of Examples 51 and 103 were sequentially followed to give the desired product as a colorless powder.

b) 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-[4-(3,5-bistrifluoromethylphenoxy)phenyloxy]ethyl-1,3,2-dioxasilane

[0225] Using the hydroxy derivative obtained above, reactions were carried out in the same manner as in Reference Example 164 to obtain an iodide, which in turn was reacted with 4-[(3,5-bistrifluoromethyl)phenoxy]phenol to give the desired product as a colorless amorphous.

[0226] ¹H-NMR(400 MHz, CDCl₃) δ 1.08(9H, s), 1.11(9H, s), 1.44(9H, s), 2.04(2H, br s), 4.04-4.07(4H, br), 4.42(2H, d, J=11.2 Hz), 5.10(1H, br s), 6.92(2H, d, J=8.5 Hz), 7.00(2H, d, J=8.5 Hz), 7.32(2H, s), 7.52(1H, s)

[0227] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-[4-(3-(1-hydroxyethyl)phenoxy)phenyl]propyl-1,3,2-dioxasilane

[0228] The compound of Example 125 (126 mg) was dissolved in THF (3.0 mL) and the solution was cooled to −78° C. under argon. A 1 mol/L methyllithium-ether solution (0.252 mL) was added to the solution and the temperature of the mixture was slowly raised to 0° C. A 5% aqueous solution of citric acid was added to the reaction mixture and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure. The residue was purified by silica gel column chromatography (hexane:ethyl acetate=5:1) to obtain the desired product (90.7 mg) as a colorless oil.

[0229] ¹H-NMR(400 MHz, CDCl₃) δ 1.05(9H, s), 1.07(9H, s), 1.42(9H, s), 1.48(3H, d, J=6.3 Hz), 1.55(4H, br s), 1.78(1H, m), 2.56(2H, t, J=6.8 Hz), 3.90(2H, d, J=11.7 Hz), 4.23(2H, d, J=11.7 Hz), 4.87(1H, q, J=6.5 Hz), 4.91(1H, br s), 6.86-6.89(1H, m), 6.92(2H, d, J=8.8 Hz), 7.03(1H, t, J=2.0 Hz), 7.07-7.12(3H, m), 7.29(1H, t, J=8.3 Hz)

[0230] 5-t-butoxycarbonylamino-2,2-di-t-butyl-5-[4-(3-phenetyl)phenoxy]phenyl]propyl-1,3,2-dioxasilane

[0231] Benzylphosphonylchloride (152 mg) was dissolved in THF (2 mL) and sodium-t-butoxide (37.6 mg) was added to the solution at 0° C. The mixture was stirred for 30 minutes at room temperature and was again cooled to 0° C., at which time a THF solution (2 mL) of the compound of Example 125 (202 mg) was added. The reaction mixture was stirred for 1 hour at this temperature and for additional 1 hour at room temperature, followed by addition of a 5% aqueous solution of citric acid. The mixture was then extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure. The residue was purified by silica gel column chromatography (hexane:ethyl acetate=30:1) to give a styryl derivative as a colorless oil. The styryl derivative so obtained was reduced by catalytic reduction as in Reference Example 81 to obtain the desired product (168 mg) as a colorless oil.

[0232] ¹H-NMR(400 MHz, CDCl₃) δ 1.05(9H, s), 1.07(9H, s), 1.43(9H, s), 1.57(4H, br s), 2.56(2H, t, J=7.1 Hz), 2.90(4H, m), 3.90(2H, d, J=11.2 Hz), 4.23(2H, d, J=11.2 Hz), 4.92(1H, br s), 6.79-6.83(2H, m), 6.88(2H, d, J=8.3 Hz), 6.89-6.92(1H, m), 7.09(2H, d, J=8.3 Hz), 7.14-7.24(4H, m), 7.25-7.29(2H, m)

[0233] 2-amino-2-[4-(3,5-bistrifluoromethylphenoxy)phenyl]propyl-1,3-propanediol hydrochloride

[0234] Ethyl acetate (20 mL) containing 3 mol/L hydrochloric acid was added to a methanol solution (10 mL) of the compound of Example 93 (1.28 g) and the mixture was stirred overnight at room temperature. The solvent was removed by distillation under reduced pressure. A mixture of ethyl acetate and hexane (ethyl acetate:hexane=1:1) was added to the residue and the crystals were collected by filtration. After drying, the desired product (1.07 g) was obtained as a colorless powder.

[0235] Alternatively, the compound of Example 124 was used in the reaction process of Example 150 to give the same product.

[0236] FABMS:438 ([M+H]+)

[0237] ¹H-NMR (400 MHz, DMSO-d₆) δ 1.55-1.58(4H, br), 2.58(2H, t, J=6.8 Hz), 3.40-3.47(4H, m), 5.31(1H, br), 7.13(2H, d, J=8.3 Hz), 7.31(2H, d, J=8.3 Hz), 7.56(2H, s), 7.76(1H, br), 7.83(1H, s).

[0238] Melting point=194-196° C.

[0239] Elemental analysis (%): C₂₀H₂₁F₆NO₃·HCl

[0240]

	C	H	N
	Calcd.	50.70	4.68	2.96
	Found	50.70	4.66	2.91

[0241] 2-amino-2-[4-(3-phenylpropyloxyphenoxy)phenyl]propyl-1,3-propanediol hydrochloride

[0242] The compound of Example 138 was reduced by catalytic reduction as in Reference Example 81. Subsequently, the reaction processes of Example 147 were followed to give the desired product as a colorless powder.

[0243] Melting point: 95-98° C.

[0244] FABMS: 436 ([M+H]+)

[0245] ¹H-NMR (400 MHz, DMSO-d₆) δ 1.56 (4H, br), 1.97(2H, quintet, 7.3 Hz), 2.49-2.53(2H, m), 3.39-3.46(4H, m), 3.92(2H, t, J=7.3 Hz), 5.30(1H, br), 6.47-6.49(2H, m), 6.66-6.69(1H, m), 6.95(2H, d, J=8.8 Hz), 7.12-7.29(8H, m), 7.68-7.72(2H, m)

[0246] 2-amino-2-[4′-(3,5-bistrifluoromethylphenoxy)cinnamyl]-1,3-propanediol hydrochloride

[0247] Using the compound of Example 100, reactions were carried out in the same manner as in Example 147 to obtain the desired product as a colorless powder.

[0248] Melting point=203-206° C.

[0249] FABMS: 436 ([M+H]+)

[0250] ¹H-NMR (400 MHz, DMSO-d₆) δ 3.32(2H, d, J=7.5 Hz), 3.48(4H, br), 6.23(1H, dt, J=15.5, 7.5 Hz), 6.53(1H, d, 15.5 Hz), 7.17(2H, d, J=8.8 Hz), 7.52(2H, d, J=8.8 Hz), 7.60(2H, s), 7.85(1H, s)

[0251] 2-amino-2-[4-(3-isopropylphenoxy)phenyl]propyl-1,3-propanediol hydrochloride

[0252] The compound of Example 109 (188 mg) was dissolved in THF (3.0 mL) and a 1 mol/L tetrabutylammoniumfluoride-THF solution (1.61 mL) was added to the solution. The mixture was stirred for 2 hours at room temperature. Subsequently, water was added to the mixture and the mixture was extracted with ethyl acetate. The organic phase was sequentially washed with water and a saturated aqueous solution of sodium chloride and was dried with anhydrous sodium sulfate. The solvent was then removed by distillation under reduced pressure. The residue was purified by silica gel column chromatography (hexane:ethyl acetate=3:2) to obtain the diol as a colorless oil. The diol so obtained was treated in the same manner as in Example 147 to give the desired product (107 mg) as a colorless amorphous.

[0253] FABMS: 344 ([M+H]+)

[0254] ¹H-NMR (400 MHz, DMSO-d₆) δ 1.17(6H, d, J=6.8 Hz), 1.55(4H, br s), 2.53(2H, br), 2.81-2.89(1H, m), 3.39-3.49(4H, m), 5.30(2H, t, J=5.1 Hz), 6.71(1H, dd, J=8.3, 2.4 Hz), 6.87(1H, t, J=2.0 Hz), 6.91(2H, d, J=8.8 Hz), 6.99(1H, d, J=8.3 Hz), 7.19(2H, d, J=8.8 Hz), 7.26(1H, t, J=8.3 Hz), 7.71(3H, br s)

[0255] The compounds of Examples 110 through 123 and the compounds of Examples 145 and 146 were treated in the same manner as in Example 150 above to synthesize the compounds shown in Table 15 below:

[0256]

TABLE 15
					FABMS	Melting
Example	R1	R2	Yield (%)	Characteristics	[M + H]+	Point (° C.)
151	F	H	88	Colorless powder	320	131-133
152	Cl	H	88	Colorless powder	336	125-127
153	Br	H	88	Colorless powder	380	154-156
154	Me	H	92	Pale brown amorphous	316
155	Ph	H	87	Colorless powder	378	164-166
156	MeO	H	83	Pale brown amorphous	332
157	EtO	H	88	Colorless powder	346	115-117
158	CF3O	H	86	Pale brown amorphous	386
159	CH2OH	H	84	Colorless powder	332	180-182
160	Ac	H	85	Yellow amorphous	344
161	NO2	H	(9)	Pale yellow amorphous	347
162	CN	H	92	Pale yellow oil*	327
163	F	F	79	Pale yellow amorphous	338
164	Cl	Cl	82	Pale yellow powder**	370	75-77
165	Ph(CH2)2	H	85	Colorless powder	406	165-167
166	MeCH(OH)	H	85	Yellow amorphous	346
In the parentheses( ), shown is the total yield from the previous table.
The mark “*” means it was isolated as a CF3CO2H salt.
The mark “**” means it was isolated as free form.

[0257] 2-amino-2-[4-(3,5-bistrifluoromethylphenoxy)phenoxy]ethyl-1,3-propanediol hydrochloride

[0258] Using the compound of Example 144, reactions were carried out in the same manner as in Example 150 to obtain the desired product as a colorless powder.

[0259] Melting point=151-155° C.

[0260] FABMS: 440 ([M+H]+)

[0261] ¹H-NMR (400 MHz, DMSO-d₆) δ 2.04(2H, t, J=6.5 Hz), 3.54(4H, s), 4.11(2H, d,J=6.5 Hz), 7.04(2H, d, J=9.2 Hz), 7.19(2H, d, J=9.2 Hz), 7.50(2H, s), 7.80(1H, s)

[0262] The compounds of Examples 96 and 126 through 129 were treated in the same manner as in Example 150 above to synthesize the compounds shown in Table 16 below:

[0263]

TABLE 16
						FABMS	Melting
Example	R1	R2	n	Yield (%)	Characteristics	[M + H]+	point (° C.)
168	Cl	Cl	0	80	Colorless powder	342	110-111
169	Cl	Cl	1	99	Pale yellow amorphous	356
170	Cl	Cl	3	89	Colorless amorphous	384
171	CF3	CF3	1	81	Colorless powder	424	116-118
172	CF3	CF3	3	96	Colorless amorphous	452

[0264] 2-amino-2-[4-(3-benzyloxyphenoxy)-2-chlorophenyl]propyl-1,3-propanediol hydrochloride

[0265] The compound of Example 51 was treated in the same manner as in Example 147 to obtain the desired product as a colorless powder.

[0266] FABMS: 442 ([M+H]+)

[0267] ¹H-NMR (400 MHz, DMSO-d₆) δ 1.58(4H, br s), 2.63(2H, br s), 3.39-3.45(4H, m), 5.08(2H, s), 5.31(2H, br), 6.56(1H, dd, J=8.3, 2.4 Hz), 6.66(1H, t, J=2.4 Hz), 6.83(1H, dd, J=8.3, 2.4 Hz), 6.94(1H, dd, J=8.3, 2.4 Hz), 7.05(1H, d, J=2.4 Hz), 7.28-7.43(7H, m), 7.71(3H, br)

[0268] Melting point=105-106° C. (EtOH-iPr20)

[0269] Elemental analysis(%): C₂₅H₂₈ClNO₄·HCl

[0270]

	C	H	N
	Calcd.	62.76	6.11	2.93
	Found	62.76	6.05	2.92

[0271] The compounds of Examples 52 through 91, 94, 95, 107, 108, and 130 through 143 were treated in the same manner as in Example 147 to synthesize the compounds shown in Tables 17 and 18 below:

[0272]

TABLE 17
							FABMS	Melting
Example	R1	R2	R3	R4	Yield (%)	Characteristics	[M + H]+	point (° C.)
174	CF3	H	H	H	89	Colorless powder	370	146-148
175	CF3	H	MeO	H	100	Colorless oil	400
176	CF3	H	H	MeO	92	Colorless amorphous	400
177	CF3	H	Cl	H	100	Colorless powder	404	120-122
178	CF3	H	H	Cl	100	Colorless amorphous	404
179	CF3	H	H	PhCH2O	85	Colorless powder	476	120-123
180	CF3	H	CF3	H	99	Colorless powder	438	124-128
181	CF3	H	H	CF3	90	Colorless amorphous	438
182	CF3	CF3	H	Cl	79	Colorless powder	472	123-125
183	CF3	Ph(CH2)2	H	H	87	Colorless powder	474	110-112
184	CF3	H	H	F	85	Colorless oil	388
185	Ph(CH2)2	Ph(CH2)2	H	H	96	Colorless amorphous	510
186	Ph(CH2)2	H	H	Cl	91	Colorless amorphous	440
187	Ph(CH2)2	H	H	CF3	94	Colorless amorphous	474
188	Ph(CH2)2	Ph(CH2)2	H	Cl	93	Colorless amorphous	544
189	Ph(CH2)2	Ph(CH2)2	H	CF3	93	Colorless amorphous	578
190	PhCH2O	H	H	H	91	Colorless amorphous	408
191	PhCH2O	PhCH2O	H	H	100	Colorless powder	514	92-95
192	PhCH2O	PhCH2O	H	Cl	100	Colorless amorphous	548
193	PhCH2O	Cl	H	Cl	91	Colorless powder	476	89-91
194	PhCH2O	H	H	Br	98	Colorless amorphous	488
195	PhCH2O	H	H	CF3	100	Colorless powder	476	72-76
196	PhCH2O	H	H	Ph	98	Colorless amorphous	484
197	PhCH2O	CF3	H	H	91	Colorless amorphous	476
198	PhCH2O	CF3	H	Cl	94	Colorless powder	510	114-118
199	t-Bu	H	H	H	100	Colorless amorphous	358
200	MeS	H	H	H	89	Colorless amorphous	348
201	n-C5H11	H	H	H	99	Colorless amorphous	372
202	n-C7H15	H	H	H	74	Yellow amorphous	400
203	i-Pr	iPrO	H	H	93	Colorless amorphous	402
204	i-Pr	iPrO	H	Cl	97	Colorless amorphous	436
205	i-Pr	i-Pr	H	Cl	95	Colorless amorphous	420
206	Cl	Cl	H	Cl	92	Colorless amorphous	404
207	PhCH2S	H	H	H	100	Colorless amorphous	424
208	PhCH2S	H	H	Cl	100	Colorless amorphous	458
209	Et	H	H	H	87	Pale yellow amorphous	330
210	i-Bu	H	H	H	92	Colorless amorphous	358
211*	OH	H	H	H	98	Colorless powder	318	174-176
212	i-PrO	H	H	H	94	Colorless amorphous	360
213	PhO	H	H	H	100	Colorless amorphous	394
The mark “*” means the step was carried out after catalytic reduction of the compound of Example 68.

[0273]

TABLE 18
							FABMS	Melting
Example	R1	R2	R3	R4	Yield (%)	Characteristics	[M + H]+	point ° C.
214		H	H	H	100	Colorless powder	476	89-92
215		H	H	H	85	Colorless amorphous	409
216		H	H	H	93	Colorless powder	458	170-173
217	Ph2CHO	H	H	H	91	Colorless powder	484	153-156
218	Ph(CH2)2O	H	H	H	90	Colorless amorphous	422
219		H	H	H	100	Colorless amorphous	434
220	PhOCH2	H	H	H	97	Colorless powder	408	119-122
221	MeSO	H	H	H	100	Colorless amorphous	364
222	MeSO2	H	H	H	100	Colorless powder	380	147-150
223**	CF3	H	H	OH	97	Colorless amorphous	386
224		H	H	Cl	100	Colorless powder	476	94-96
225		H	H	Cl	83	Colorless powder	510	92-95
226		H	H	Cl	94	Colorless amorphous	472
227		H	H	Cl	100	Colorless powder	456	84-86
228		H	H	Cl	76	Colorless powder	510	88-91
229	PhCH2O	H	H	i-Pr	97	Colorless amorphous	450
230		H	H	H	90	Colorless powder	414	125-127
231		H	H	H	100	Colorless powder	442	195-197
232		H	H	H	100	Colorless powder	442	130-132
233		H	H	H	100	Colorless powder	442	94-96
The mark “**” means the step was carried out after catalytic reduction of the compound of Example 57.

[0274] Using the compounds of Reference Examples 241 through 250, reactions were carried out in the same manner as in Example 1 to synthesize the compounds below:

[0275]

TABLE 19
Example	R1	R2	R3	R4	Characteristics	Yield (%)
234	Ph(CH2)2	c-CF3	H	Cl	Colorless oil	93
235	PhCH2O	c-H	H	Me	Colorless oil	100
236	PhCH2O	c-H	H	Et	Colorless oil	72
237	PhCH2O	c-H	H	SMe	Colorless oil	—
238	PhO	c-H	H	Cl	Colorless oil	92
239	CF3	a-Cl	H	H	Colorless oil	100
240	CF3	b-Cl	H	H	Colorless oil	94
241	CF3	d-Cl	H	H	Pale yellow oil	72
242	CF3	c-Cl	H	H	Pale yellow oil	41
243	PhCH2O	c-H	H	F	Colorless oil	—
The mark “—” means yield is shown in Table 20 as a total yield.

[0276] Ethyl 4-[4-(3-benzyloxyphenoxy)-2-chloro]phenyl-2-t-butoxycarbonylamino-2-ethoxycarbonylbutyrate

[0277] Using the compound of Example 261, reactions were carried out in the same manner as in Example 1 to obtain the desired product as a colorless oil.

[0278] ¹H-NMR(400 MHz, CDCl₃) δ 1.23-1.32(6H, m), 1.45(9H, s), 2.59(4H, br), 4.22-4.34(4H, m), 5.03(2H, s), 6.58-6.62(2H, m), 6.75(1H, dd, J=8.3 Hz, 2.4 Hz), 6.83(1H, dd, J=8.3 Hz, 2.4 Hz), 6.98(1H, d, J=2.4 Hz), 7.12(1H, d, J=8.3 Hz), 7.23(1H, t, J=8.3 Hz), 7.30-7.42(5H, m)

[0279] Ethyl 6-[4-(3-benzyloxyphenoxy)-2-chloro]phenyl-2-t-butoxycarbonylamino-2-ethoxycarbonylhexanoate

[0280] Using the compound of Example 262, reactions were carried out in the same manner as in Example 1 to obtain the desired product as a colorless oil.

[0281] ¹H-NMR (400 MHz, CDCl₃) δ 1.24(6H, t, J=7.3 Hz), 1.43(9H, s), 1.58-1.67(4H, m), 2.33(2H, br), 2.67(2H, t, J=7.8 Hz), 4.18-4.32(4H, m), 5.03(2H, s),5.95(1H, br s), 6.57-6.60(1H, m), 6.62(1H, t, J=2.4 Hz), 6.74(1H, dd, J=8.3 Hz, 2.4 Hz), 6.83(1H, dd, J=8.3 Hz, 2.4 Hz), 6.99(1H, d, J=2.4 Hz), 7.12(1H, d, J=8.3 Hz), 7.23(1H, t, J=8.3 Hz), 7.30-7.42(5H, m)

[0282] Using the compounds of Examples 234 through 243, reactions were carried out in the same manner as in Example 51 to synthesize the compounds below:

[0283]

TABLE 20
Reference
example	R1	R2	R3	R4	Characteristics	Yield (%)
246	Ph(CH2)2	c-CF3	H	Cl	Colorless oil	46
247	PhCH2O	c-H	H	Me	Colorless oil	75
248	PhCH2O	c-H	H	Et	Colorless oil	61
249	PhCH2O	c-H	H	SMe	Colorless oil	38
250	PhO	c-H	H	Cl	Colorless oil	76
251	CF3	a-Cl	H	H	Colorless oil	57
252	CF3	b-Cl	H	H	Colorless oil	62
253	CF3	d-Cl	H	H	Colorless oil	37
254	CF3	c-Cl	H	H	Colorless oil	51
255	PhCH2O	c-H	H	F	Colorless oil	34

[0284] 2-[4-(3-benzyloxyphenoxy)-2-chlorophenyl]ethyl-2-t-butoxycarbonylamino-1,3-propanediol

[0285] The compound of Example 244 was treated in the same manner as in Example 51 to obtain the desired product as a colorless powder.

[0286] ¹H-NMR (400 MHz, CDCl₃) δ 1.46(9H, s), 1.83-1.87(2H, m), 2.69-273(2H, m), 3.35(2H, br), 3.67(2H, dd, J=11.7 Hz, 5.9 Hz), 3.92(2H, dd, J=11.7 Hz, 4.9 Hz), 5.03(2H, s), 5.10(1H, s), 6.57-6.62(2H, m), 6.75(1H, dd, J=8.3 Hz, 2.4 Hz), 6.85(1H, dd, J=8.3 Hz, 2.4 Hz), 7.00(1H, d, J=2.4 Hz), 7.17(1H, d,J=8.3 Hz), 7.24(1H, t, J=8.3 Hz), 7.32-7.42(5H, m)

[0287] 2-[4-(3-benzyloxyphenoxy)-2-chlorophenyl]butyl-2-t-butoxycarbonylamino-1,3-propanediol

[0288] The compound of Example 245 was treated in the same manner as in Example 51 to obtain the desired product as a colorless oil.

[0289] ¹H-NMR (400 MHz, CDCl₃) δ 1.44(9H, s), 1.61(6H, br), 2.70(2H, t, J=7.3 Hz), 3.46(2H, br), 3.57-3.60(2H, m), 3.84(2H, d, J=9.8 Hz), 4.92(1H, s), 5.03(2H, s), 6.59-6.63(2H, m), 6.73-6.76(1H, m), 6.84(1H, dd, J=8.3 Hz, 2.4 Hz), 7.00(1H, d, J=2.4 Hz), 7.13(1H, d, J=8.3 Hz), 7.24(1H, t, J=8.3 Hz), 7.23-7.43(5H, m)

[0290] Using the compounds of Examples 246 through 255, reactions were carried out in the same manner as in Example 147 to synthesize the compounds below:

[0291]

TABLE 21
Example	R1	R2	R3	R4	Yield (%)	Characteristics	FABMS [M + H]+	Melting point (° C.)
258	Ph(CH2)2	c-CF3	H	Cl	96	Pale yellow amorphous	508
259	PhCH2O	c-H	H	Me	92	Yellow amorphous	422
260	PhCH2O	c-H	H	Et	100	Pale yellow amorphous	436
261	PhCH2O	c-H	H	SMe	100	Colorless amorphous	454
262	PhO	c-H	H	Cl	92	Colorless amorphous	428
263	CF3	a-Cl	H	H	93	Pale yellow amorphous	404
264	CF3	b-Cl	H	H	99	Colorless powder	404	133-136
265	CF3	d-Cl	H	H	78	Pale yellow amorphous	404
266	CF3	c-Cl	H	H	76	Colorless powder	404	180-182
267	PhCH2O	c-H	H	F	100	Colorless powder	426	71-73

[0292] 2-amino-2-[4-(3-benzyloxyphenoxy)-2-chlorophenyl]ethyl-1,3-propanediol hydrochloride

[0293] The compound of Example 256 was treated in the same manner as in Example 147 to obtain the desired product as a colorless powder.

[0294] FABMS: 428 ([M+H]+)

[0295] ¹H-NMR(400 MHz, DMSO-d₆) δ 1.75-1.79(2H, m), 2.68-2.72(2H, m), 3.51-3.55(4H, m), 5.08(2H, s), 5.40(2H, t, J=4.9 Hz), 6.57(1H, dd, J=8.3 Hz, 2.4 Hz), 6.67(1H, t, J=2.4 Hz), 6.83(1H, dd, J=8.3 Hz, 2.4 Hz), 6.95(1H, dd, J=8.3 Hz, 2.4 Hz), 7.05(1H, d, J=2.4 Hz), 7.27-7.43(7H, m), 7.88(3H, br)

[0296] Melting point=150-152° C.

[0297] 2-amino-2-[4-(3-benzyloxyphenoxy)-2-chlorophenyl]butyl-1,3-propanediol hydrochloride

[0298] The compound of Example 257 was treated in the same manner as in Example 147 to obtain the desired product as a pale yellow amorphous.

[0299] FABMS: 456 ([M+H]+)

[0300] ¹H-NMR(400 MHz, DMSO-d₆) δ 1.30-1.40(2H, m), 1.46-1.60(4H, m), 2.64(2H, t, J=7.8 Hz), 3.39-3.48(4H, m), 5.08(2H, s), 5.32(2H, t, J=5.4 Hz), 6.57(1H, dd, J=8.3 Hz, 2.4 Hz), 6.67(1H, t, J=2.4 Hz), 6.82(1H, dd, J=8.3 Hz, 2.4 Hz), 6.91(1H, dd, J=8.3 Hz, 2.4 Hz), 7.03(1H, d, J=2.4 Hz), 7.27-7.43(7H, m), 7.76(3H, br)

[0301] Melting point=95-97° C.

[0302] The following experiments were conducted to prove the effectiveness of the compounds of the present invention.

<Experiment 1>

Ability of Test Compounds to Suppress Host vs Graft Rejection in Mice

[0303] This experiment was performed according to the method described in Transplantation, 55, No.3 (1993): 578-591. Spleens were collected from 9 to 11 week old male BALB/c mice (CLEA JAPAN Inc., CHARLES RIVER JAPAN Inc., or JAPAN SLC Inc.). The spleens were placed in a phosphate-buffered saline (PBS(−), NISSUI PHARMACEUTICAL Co., Ltd.) or in an RPMI-1640 medium (GIBCO INDUSTRIES Inc., or IWAKI GLASS Co., Ltd.) and were either passed through a stainless steel mesh, or gently pressed between two slide glasses and then passed through a cell strainer (70 μm, Falcon), to form a cell suspension. The suspension was then centrifuged and the supernatant was discarded. An ammonium chloride-Tris isotonic buffer was added to the suspension to lyse erythrocytes. The cells were then centrifuged and washed three times in PBS (−) or RPMI-1640 medium and were resuspended in an RPMI-1640 medium. To this suspension, mitomycin C (KYOWA HAKKO KOGYO Co., Ltd.) was added to a final concentration of 25 μg/mL and the suspension was incubated for 30 minutes at 37° C. in a 5% CO₂ atmosphere. The cells were again centrifuged and washed in PBS (−) or RPMI-1640 medium and were resuspended in an RPMI-1640 medium so that the medium would contain 2.5×10⁸ cells/mL. This suspension served as a “stimulation cell suspension.” Using a 27G needle along with a microsyringe (Hamilton), 20 μL (5×10⁶ cells/mouse) of the stimulation cell suspension was subcutaneously injected into the right hind footpad of 7 to 9 week old male C3H/HeN mice (CLEA JAPAN Inc., CHARLES RIVER JAPAN Inc., or JAPAN SLC Inc.). A group of mice was injected with RPMI-1640 medium alone to serve as normal control. 4 days after the injection, right popliteal lymph nodes were collected and were weighed on a Mettler AT201 electronic scale (METTLER TOLEDO Co., Ltd.). Each animal was intraperitoneally administered a test compound once a day for four consecutive days starting on the day of the injection of the stimulation cells (i.e., total of 4 times). Controls were administered a vehicle that has the same composition as that used in the preparation of the test compounds. The results are shown in Table 22 below:

[0304]

TABLE 22
Example	Dose	Inhibition
No.	(mg/kg)	(%)
147	10	82
148	3	78
150	10	78
157	10	50
164	10	82
166	10	91
169	10	86
170	10	71
171	10	79
172	10	78
173	3	100
174	10	62
175	10	64
176	10	63
177	10	71
178	10	82
181	10	96
182	3	78
183	3	102
184	3	64
185	3	63
186	1	87
187	3	76
188	3	68
189	3	54
190	10	83
191	3	95
192	0.3	93
194	0.3	85
195	3	69
197	3	93
198	3	92
200	10	50
202	10	92
203	10	77
204	10	79
205	10	84
206	10	76
207	3	69
208	3	90
209	10	71
210	10	76
212	10	78
213	3	68
214	3	79
215	3	76
217	3	66
218	3	92
219	3	53
220	3	77
223	10	63
224	0.3	76
225	0.03	70
226	3	89
227	3	93
228	0.3	74
230	3	67
231	3	83
232	3	92
233	3	85

<Experiment 2>
Ability of Test Compounds to Suppress Delayed-type Hypersensitivity in Mice.

[0305] This experiment was performed according to the method described in Methods in Enzymology, 300 (1999): 345-363. 1-fluoro-2,4-dinitrobenzene (DNFB, NACALAI TESQUE Inc.) was dissolved in a mixture of acetone and olive oil (acetone:olive oil=4:1) to a concentration of 1% (v/v). 10 μL of this 1% DNFB solution was applied to the footpad of each hind leg of male BALB/c mice (JAPAN SLC Inc. or CHARLES RIVER JAPAN Inc.) for sensitization. The sensitization was done for 2 consecutive days (day 0 and day 1). On day 5, the ears of the mice were challenged with the antigen to induce delayed-type hypersensitive responses: First, the thickness of each ear was measured by the dial thickness gauge G (0.01-10 mm, OZAKI MFG Co., Ltd.). Next, a test compound was administered. 30 minutes after the administration, 10 μL of a 0.2% (v/v) DNFB solution was applied to the inner and outer surfaces of the right ear of each animal for antigen challenge. The left ear of each animal was challenged with the solvent alone. 24 hours after the challenge, the increase in the ear thickness was measured for each ear and the difference between the right and the left ears was determined for each individual. The test compound was dissolved, or suspended, in an ultra pure water and was orally administered at a dose of 0.1 mL/10 g of body weight. A control group was administered ultra pure water alone. The results are shown in Table 23 below:

[0306]

TABLE 23
Example	Dose	Inhibition
No.	(mg/kg)	(%)
173	3	64
178	10	72
181	10	69
182	30	101
190	3	67
195	30	64
198	3	57

<Experiment 3>
Activities of Test Compounds on Skin Transplantation Model in Mice

[0307] Effects of the test compounds were examined on skin transplantation model in mice. The experimental procedure was referred to the method described in Journal of Experimental Biology, 28, No.3 (1951); 385-405.

[0308] First, dorsal skin from male DBA/2 mice were stripped of the fatty layer and the panniculus carnosus, and cut into circular grafts with a diameter of 8 mm. Next, graft bed, a circular area, approximately 8 mm in diameter, was prepared in the back of anesthetized male BALB/c mice with a scalpel while the skin was pinched by forceps. Each graft obtained from the DBA/2 mice was placed on the graft bed formed in the backs of the BALB/c mice and was secured with a strip of adhesive bandage while held down from the top. 6 days after transplantation, the bandage was removed and the graft was subsequently observed everyday. The activity of each compound was evaluated based on the length of the survival period, which is defined as the number of days for rejection. Each test compound was dissolved in ultra pure water and was orally administered once a day, starting from the day of transplantation. In a similar manner, the control group was administered ultra pure water alone.

[0309] The results are shown in FIGS. 1 through 8.

[0310] As can be seen from the results, the compounds of the present invention represented by the general formula (1) have proven effective in animal model.

[0311] As set forth, the present invention has been devised in recognition of the fact that novel diaryl derivatives, in particular those in which one of the aryl groups includes, at its para-position, a carbon chain with an aminopropanediol group and the other aryl group includes a substituent at its meta-position, exhibit strong immunosuppressive effects. Acting as effective immunosuppressors, the compounds of the present invention have a strong potential as a prophylactic or therapeutic agent against rejection in organ or bone marrow transplantation, autoimmune diseases, rheumatoid arthritis, psoriasis, atopic dermatitis, bronchial asthma, pollinosis and various other diseases.