US 5,374,424 AGrant

Multivalent Felv-Infected Feline Vaccine

Issue Date:1994-12-20

•12 Claims

Abstract

A multivalent FeLV-infected feline vaccine composed of (1) a small but immunologically effective amount of inactivated feline leukemia virus; (2) a small but effective amount of an inactivated virus selected from the group consisting of Feline Rhinotracheitis Virus, Feline Calici Virus and Feline Panleukopenia Virus; and (3) a pharmaceutically acceptable immunologica adjuvant. Components (1) and (2) may be inactivated by any of the known techniques. The inactivated feline leukemia virus must be from subgroup A and is the Rickard isolate of the virus designated FeLV-A.sub.R1. This combination vaccine is effective in preventing Viremia, leukemia-associated syndromes, and deaths in cats caused by Feline Leukemia Virus, Feline Rhinotracheitis Virus, Feline Calici Virus and Feline Panleukemia Virus infections.

Metadata

Assignee

Miles Inc.

Inventors

William H. Kelsey
Edmund P. Bass

Application Information

Application Number:US 2843099

Filing Date:1988-12-14

Priority Date:1986-10-03

Art Unit:185

Classifications

IPC:

A61K 3912C12N 700

Field of Search:

42443589235.1;236;238;240.1

Patent Drawings

This patent does not have any drawings.

Description

BACKGROUND OF THE INVENTION

Feline leukemia virus (FeLV) is a retrovirus consisting of three subgroups designated A, B, and C. The FeLV subgroups were identified initially by viral interference and cross-neutralization. Sarma & Log, Virology (1973) 54:160-169; see also, Russell & Jarrett, Int'l J. Cancer (1978) 21: 768-778; and Jarrett (1980) in Feline Leukemia Virus-Developments in Cancer Research Vol. 4, Hardy et al., eds., Elsevier North Holland, Inc., pp. 473-479. Feline leukemia virus is discussed also in Jarrett & Russell, Int'l J. Cancer (1978) 21: 466-472; Sarma et al., J. Nat'l Cancer Inst. (1978) 60: 871-874; and Schaller & Olsen Infect. Immun. (1975) 12: 1405-1510.

FeLV is infectious in cats. It is responsible for a number of diseases, including lymphoplastic or aplastic anemia, myelosuppression, thymic atrophy, and thrombocytopenia, as well as reproductive failure, e.g., abortion fetal resorption, and stillbirths. Neoplastic manifestations of FeLV infection, such as lymphosarcoma, account for only a small portion of the morbidity and mortality caused by FeLV. FeLV infection in cats, however, also causes suppression of the immune system, thereby exposing the animal to opportunistic infections by a variety of microorganisms.

Vaccines have been developed for FeLV. See, e.g., U.S. Pat. No. 4,332,793 to Olson, U.S. Pat. No. 4,264,587 to Pedersen et al., U.S. Pat. No. 4,117,112 to Jarrett et al., U.S. Pat. No., 4,086,134 to Jarrett et al., U.S. Pat. No. 4,034,081 to Jarrett et al., U.S. Pat. No. 3,966,907 to Jarrett et al., and Belgian Patent Application R 64 417 DG to the University of Glasgow.

In particular, a FeLV vaccine may be prepared from inactivated FeLV virus and from envelope glycoprotein (gp70), the antigen with which it is thought protective antibody must react. Such viral material generally may be produced by culturing infected feline cell lines, but it has been impossible to predict whether a particular feline cell line will produce vital material of appropriate quality in quantities sufficient to be useful for commercial production of FeLV vaccines. Moreover, a cell line which satisfactorily produces viral material for a given strain of FeLV may not produce adequate protection against other strains or may produce vital material that for other reasons is ineffective in vaccine formulations.

Thus, there is a continuing need to develop new systems for producing FeLV viral material that can be used in formulating commercially useful FeLV vaccines. It should also be noted that FeLV viral material is useful in the preparation of diagnostic reagents, and thus, new cell lines for its production are needed for that purpose as well.

There is also a continuing need for development of multivalent vaccines for FeLV which also successfully vaccinate for other viruses which commonly infect cats. Such multivalent vaccines must not only be effective in development of the desired immune response but the admixture of the various inactivated viruses must be such that each is compatible with the other and the adjuvant.

The objectives of the present invention include development of safe and effective monovalent and multivalent FeLV vaccines useful for vaccination of cats against FeLV virus and certain other common, infectious viruses of cats.

SUMMARY OF THE INVENTION

Our earlier invention provided for Crandell Feline Kidney (CRFK) cells infected with a Rickard isolate of FeLV, i.e., FeLV-A.sub.R1, with the infected cells being capable of producing FeLV virus in amounts sufficient for formulating safe and effective FeLV vaccines. This invention improves on our earlier one by providing a multivalent or combination FeLV vaccine.

This invention also provides for a process for producing FeLV vital material, which process comprises infecting CRFK cells with an FeLV virus, such as FeLV-A.sub.R1, cultivating the infected CRFK cells, recovering the vital material, and using it to provide multivalent FeLV vaccines.

Surprisingly, it has now been found that the FeLV-infected Crandell cell line of the subject invention stably yields high titers of FeLV which can be used in safe and efficacious multivalent FeLV vaccines. Particularly, it produces 10 to 100 times higher titers of gp70 than do normal feline embryo cells.

DETAILED DESCRIPTION OF THE INVENTION

The feline cell line employed in the present invention is the Crandell Feline Kidney (CRFK) line obtained from the American Type Culture Collection (ATCC CCL94). CRFK cells are described in Crandell et al., In Vitro (1973) 9:176-185.

The CRFK cell line is infected with a Rickard isolate of FeLV, i.e., FeLV-A.sub.R1. Upon culturing, the infected cell line, designated ACFL, becomes chronically infected and produces viral material at a rate of at least 10.sup.3 to 10.sup.7 focus-forming units per milliliter. The FeLV virus produced by the cells exhibits a titer of 10.sup.5 preferably 10.sup.6 focus-forming units per milliliter (FFU/ml) in one to three weeks, and at least 2.times.10.sup.6 preferably 3.times.10.sup.6 FFU/ml thereafter.

On the other hand, when infected with another Rickard isolate, FeLV-A.sub.R2, the CRFK cells produced less viral material, and the vital material produced was less effective in vaccine formulations. Similarly, viral material produced from another FeLV-A.sub.R1 -infected cell line, AK-D cells, which satisfactorily produce viral material when infected with the Sarma strain of FeLV, was inadequate for formulating vaccines.

The viral material produced by the ACFL cell line may be used in a variety of vaccine formulations within the scope of this invention. The viral material is virulent for cats and must be inactivated before it is used to prepare vaccines.

The viral material may be inactivated by conventional means which maintain immunogenicity. Means of inactivation are known in the art. Particular means of inactivation which have found use herein include heat, formalin, binary ethyleneimine (BEI), ozone, and psoralen. See U.S. Pat. Nos. 4,332,793; 4,264,587; 4,117,112; 4,034,081; and 3,996,907. Preferably either BEI or psoralen inactivation will be employed. In the psoralen method the live virus is combined in an appropriate medium with the psoralen. The amount of psoralen should be sufficient to provide for a substantially complete inactivation of the virus upon irradiation with long wave ultraviolet radiation. See U.S. Pat. No. 4,545,987.

In accord with the present improvement invention the same FeLV inactivation procedure and techniques are employed as employed in our earlier invention of the monovalent vaccine. Thus, the techniques as described in our earlier filed application are wholly applicable to the present improved vaccine as well. This present vaccine, that is the improved one, involves a multivalent or combination vaccine which vaccinates for FeLV and at least one other common feline viral infection.

The other viruses which may be inactivated by use of the same general procedures described herein and included in the multivalent improved vaccine include one or more of the following viruses: Feline Rhinotracheitis Virus (FVR); Feline Calici Virus (FCV); and Feline Panleukopenia Virus (FPV). Each of these three additional viruses are common viral infections of cats. FVR provides disease symptoms similar to the common human cold. FCV provides disease symptoms of swollen and/or watering eyes and common coughing. FPV virus is a systematic infection of cats and may often be fatal.

Surprisingly, any combination of these inactivated viruses are compatible with the basic monovalent FeLV vaccine of the parent application. They are compatible not only from the standpoint of being compatible with the adjuvant but neither interferes with development of the desired antigenic response of the other. Thus, the vaccine of the present invention includes not only the basic inactivated FeLV vaccine but also one or more or all of the additional inactivated viruses, i.e. Feline Rhinotracheitis Virus, Feline Calici Virus, and Feline Panleukopenia Virus.

The dosage levels in the combination or multivalent vaccines of the present invention should generally be within the following ranges. For FeLV the preferred range is from about 6.5.times.10.sup.6 FFU equivalents per dose to 1.times.10.sup.7 FFU equivalents per dose. For FVR the preferred dosage level is from about 10.sup.6.5 TCID.sub.50 equivalents or more per dose to about 10.sup.7 TCID.sub.50 equivalents per dose. For FCV the preferred dose level ranges from about 10.sup.5.6 TCID.sub.50 equivalents or more per dose to about 10.sup.6 equivalents per dose. For FPV the preferred range is from about 10.sup.5.1 TCID.sub.50 equivalents or more per dose to 10.sup.6 equivalents per dose.

The vaccines may include a physiologically-acceptable carrier, for example, an inert carrier such as deionized water, phosphate-buffered saline, saline, or the like. The carrier may also include a physiologically-acceptable adjuvant, such as a mineral oil, vegetable oil, mineral salt, or an immunopotentiator, such a muramyl dipeptide. Such carriers and adjuvants are well known in the art.

Typically, the concentration of the FeLV virus in the vaccine formulation, either separated from or within the infected cell, will be from about 10.sup.2 to about 10.sup.9 FFU/ml. If needed, the virus may be conveniently concentrated using commercially available equipment. The total dosage generally is at least about 10.sup.2 FFU per dose, more commonly at least about 10.sup.5 per dose. It generally will not exceed about 10.sup.9 FFU per dose.

Usually, a specific dosage at a specific site will be administered in a volume in the range from about 0.1 ml to 4 ml, where the total volume administered will range from 0.5 ml to 8 ml. The number of injections and their temporal spacing may be varied, but one to three injections at one to three week intervals are usually effective.

The vaccine may be administered subcutaneously, intramuscularly, or intraperitoneally. Conveniently, the prepared vaccines will be packaged in small vials holding sufficient vaccine for one dose and having a septum for inserting a hypodermic needle.

The subject invention is further described by reference to the following example. These examples are not intended to limit the scope of the invention; rather, they are presented merely to facilitate the practice of the invention by those of ordinary skill in the art.

EXAMPLE 1

The production of FeLV virus and gp70 was compared in (1) FeLV-A.sub.R1 infected CRFK cells, (2) FeLV-A.sub.R2 infected CRFK cells, and (3) FeLV-A.sub.R1 infected AK-D cells as follows:

Infection and Culturing

CRFK cells (ATCC CCL94) were seeded at a density of approximately 5.times.10.sup.6 cells into a 75 cm.sup.2 tissue culture flask in 12 ml of MEN.sub.95 F.sup.i.sub.5 growth medium and incubated overnight at 37.degree. C. (MEN.sub.95 F.sup.i.sub.5 is Eagle's Minimum Essential Medium with Earle's salts and nonessential amino acids supplemented with 5% heat-inactivated fetal bovine serum). At 20 hours postseeding the growth medium was removed from the cell monolayer, and 2 ml of overlay medium (MEN containing 25 ug/ml of DEAE-Dextran) was added. The cells then were incubated for 1 hour at 37.degree. C. After incubation, the overlay medium was removed, and the cell monolayer was rinsed two times with 10 ml of MEN.sub.95 F.sup.i.sub.5.

The cell monolayer then was overlayed with 2 ml of FeLV-A.sub.R1 having titer of approximately 2.5.times.10.sup.4 FFU/ml. The virus was allowed to absorb for 2 hours at 37.degree. C. The multiplicity of infection (MOI) was 0.01. Following the 2 hour adsorption period, cells were fed with 12 ml MEN.sub.95 F.sup.i.sub.5 growth medium and incubated at 37.degree. C. The initial flask of CRFK cells infected with FeLV-A.sub.R1 was designated O-ACFL.

Following the initial infection, the medium was changed at three-day intervals. Confluency generally was reached on the seventh day following initial infection, i.e., day seven post-infection (p.i.), at which time the cells were subcultured at a split ratio of 1:10 in a new flask. This same process was carried out at seven-day intervals for 5 weeks, the subsequent flasks being designated 1-ACFL, 2-ACFL, etc.

The same procedure described above for FeLV-A.sub.R1 infected CRFK cells also was used to infect CRFK cells with FeLV-A.sub.R2 and to infect AK-D cells with FeLV-AR.sub.1.

At 7-day intervals p.i., samples of medium containing FeLV-A were withdrawn. The samples were titrated and assayed for p27 as described below with the results shown in Table 1 below, thereby confirming that the cell lines were chronically infected.

Recovery of Vital Material

At confluency, 250 ml MEN.sub.95 F.sup.i.sub.5 were added to each of 10 roller bottles of fifth passage ACFL cells (5-ACFL). After 72 hours the supernatant was harvested, pooled, and frozen with 10% DMSO and stored at -85.degree. C. Fresh medium was added to the roller bottles and at 144 hours the supernatant was again harvested, pooled, and frozen. The harvests were thawed, pooled, and concentrated 10-fold to a final volume of 500 ml using a Millipore Corporation Pellicon ultrafiltration cassette (100,000 MW exclusion).

The same procedure was used to concentrate virus harvested from fifth passage, FeLV-A.sub.R2 infected CRFK cells (5-CRFK). The vital material produced by the FeLV-A.sub.R1 infected AK-D cells was not recovered because the production levels of viral material were too low to be of interest for formulating vaccines.

The pooled and concentrated viral materials from the 5-ACFL and 5-CRFK cell lines were titrated and assayed for p27 as described below. They were also assayed for gp70 and tested for reverse transcriptase activity, again as described below.

Virus Titration: Determination of Focus Forming Units

Standard test cells (clone 81) were plated into 6-cm plastic petri dishes at 2.times.10.sup.5 cells/dish in growth medium (McCoy's 5A+15% heat-inactivated fetal calf serum) and incubated in a humidified CO.sub.2 incubator. Approximately 20 hours later the medium was withdrawn and 1 ml of DEAE-Dextran (DEAE-D) (25 ug/ml) in growth medium was added to each dish. The cells were then incubated at 37.degree. C. After 30 minutes the DEAE-D medium was withdrawn, and each dish was washed once with 2 ml of growth medium.

Virus inocula of 0.2 to 1.0 ml per dish were applied, the dishes were incubated, with occasional shaking, for 30 minutes at 37.degree. C., and then 5 ml of growth medium was added to each dish. On day 3 p.i., 3 ml of growth medium was added. On day 7 p.i., all growth medium was removed and replaced with 5 ml of fresh growth medium. On day 10 p.i., 3 ml of fresh growth medium was added.

Foci were read on day 12 p.i. The foci typically are 0.5 to 2 mm in diameter and consist of piled, small, dark, round cells on a dense monolayer. Generally, no retraction of the monolayer occurs in the focus area.

Further details of this procedure are set forth in Fischinger et al., J. Virol (1974) 14:177-179.

p27 Assay

Supernatants from infected cultures were assayed for FeLV group specific (p27) antigen by an ELISA (Leukassay*F, commercially available from Pitman Moore) according to the manufacturer's specified procedure.

gp70 Assay

Supernatants from infected cultures were assayed for FeLV gp70 by an indirect ELISA. A microtiter plate coated with polyclonal goat anti-FeLV gp70 antibody (NCI Repository) was used as the capturing solid phase. The antibody used to detect virus bound to the plate was a mouse anti-FeLV gp70 monoclonal antibody (AGRI). The second antibody was a peroxidase-conjugated goat anti-mouse immunoglobulin (Boehringer Mannhelm Biochemicals). The substrate used was tetramethylbenzidine in citrate buffer.

Quantitation of gp70 was based on comparison with a standard sample of purified virus. The standard virus sample was prepared by banding virions in a preformed linear 15-60% sucrose gradient by velocity sedimentation in an ultracentrifuge using standard methods. Test and standard samples were diluted serially and assayed by ELISA. The optical density of each dilution point was determined. The titer of the test sample relative to the virus standard was computed at 50% of the maximum optical density of the test sample.

Reverse Transcriptase Assay

Cell culture supernatants were tested for reverse transcriptase activity by the following procedure: Fifty microliters of 2X cocktail were added to each 50 ul aliquot of cell culture supernatant. (2X Cocktail contains 80 mM Tris-HCl1 (pH 8.2), 120 mM KCl, 1.2 mM MnAcetate, 1 OD.sub.260 poly-A, 0.01 OD.sub.260 oligodT.sub.12-18, 0.1 mM TTP, 0.4% NP-40, 3.0 mM dithiothreitol, 0.1 uCi/ul .sup.3 H-TTP in deionized water). The mixture was incubated for 30 minutes at 37.degree. C., whereupon the reaction was stopped, and the DNA was precipitated by adding 0.2 ml of 0.1M sodium polyphosphate and 0.3 ml of 20% cold trichloroacetic acid (TCA) to each tube. The tubes then were incubated for 30 minutes at 0.degree. C. Samples were collected on 2.4 cm Whatman GF/A filters. Sample tubes were washed twice with cold 5% TCA. Filters were washed twice with cold 5% TCA followed by cold 100% ethanol and then dried. The radioactivity of the viral-specific DNA was counted in 7 ml scintillation fluid in a Beckman Instruments model LS-1800 scintillation counter.

Results

The titration and various assay results are reported in Table 1. As shown therein, the ACFL line produced virus of higher titer, sooner after infection, and continued to produce virus and vital antigen at a higher sustained level than did CRFK cells infected with FeLV-A.sub.R2 or AK-D cells infected with FeLV-A.sub.R1. The infected AK-D cells did not produce virus of sufficiently high titer to be useful in vaccine production. This result was unexpected, because AK-D cells have been used successfully to produce quantities of the Sarma strain of FeLV-A sufficient for preparing experimental vaccines.

EXAMPLE 2

The production of FeLV-A.sub.R1 viral material in eighth-passage, chronically infected ACFL cells and in eleventh-passage, chronically infected normal feline embryo cells was compared. Infection, culturing, titration, and assaying were performed as described above in Example 1. The results are reported below in Table 2. As shown therein, the ACFL line produced more virus and gp70 viral antigen than did the normal feline embryo cells infected with the same virus isolate.

EXAMPLE 3

The effectiveness of vaccine formulations derived from the ACFL and FeLV-A.sub.R2 infected CRFK cell lines was compared as follows:

Vaccine Formulations

A first vaccine formulation was prepared from the pooled, concentrated vital material produced by the ACFL cells of Example 1. The concentrated virus preparation was inactivated by 0.2% formalin for approximately 48 hours at room temperature. The vaccine contained approximately 10.sup.7 FFU of virus suspension and an oil-in-water adjuvant in a 1:1 ratio.

A second vaccine formulation was prepared in the same manner from the pooled, concentrated virus produced by the FeLV-A.sub.R2 infected CRFK cells of Example 1. It contained approximately 2.4.times.10.sup.6 FFU of virus.

Feline Subjects

Eighteen cats (minimal disease cats, Liberty Labs, Liberty Corner, N.J.), nine males and nine females, 14 to 15 weeks of age, were divided into three groups of six cats each. Groups I and II were vaccinated intramuscularly twice with a 3-week interval with 1 ml, respectively, of the first and second vaccine formulations. Group III cats served as unvaccinated controls. All cats were challenged oronasally two weeks after the second inoculation with 10.sup.6 FFU of live FeLV-A.sub.R1.

Blood samples were taken weekly. The samples were assayed for FeLV antigens, by p27 ELISA as described above, and by indirect immunofluorescence, as described below. They also were tested by the virus isolation protocol described below.

Immunofluorescence Assay (IFA)

Smears of blood from vaccinated cats were tested for the presence of FeLV antigens in blood cells by indirect immunofluorescence- The first antibody was goat anti-FeLV p27 (NCI Repository); the second antibody was fluorescein-conjugated rabbit anti-goat IgG (Copper Biomedical/Cappel Laboratories). Slides were examined on a Zeiss Photomicroscope III equipped for epifluorescence.

Virus Isolation Protocol

The cats were kept in isolation. Blood samples were aseptically collected. Serum was separated by centrifugation and filtered through 0.2 um filter discs (e.g., Gelman Acrodiscs). Lymphocytes were separated by density centrifugation by diluting 1 to 1.5 ml EDTA-treated blood with 1 to 1.5 ml phosphate buffered saline (PBS), layering the suspension onto 2.5 ml Ficoll-Paque (Pharmacia), and centrifuging the tubes at 800.times.g for 30 minutes. Cells banded at the interface were harvested and washed once in PBS. Lymphocytes were resuspended in 0.2 ml of autologous serum.

An indicator cell culture was prepared by growing AK-D feline lung cells (ATCC CCL150) in F12K medium supplemented with 15% heat-inactivated fetal bovine serum and antibiotics (100 units/ml Penicillin and 100 ug/ml Streptomycin).

The AK-D cells were harvested by trypsin treatment. Approximately 5.times.10.sup.5 cells were seeded into each 25 cm.sup.2 flask with 4 ml medium. The cultures were incubated at 37.degree. C. with 5% CO.sub.2 for one day. The medium was removed, and the resuspended lymphocytes (0.2 ml) were inoculated onto the AK-D cell monolayer. Control cultures were inoculated with PBS. After the inoculum was adsorbed for 1 hour, 5 ml of medium was added. The medium was changed the next day. Thereafter, the cells were cultured at 37.degree. C. with 5% CO.sub.2, fed on day 3 or 4, and subcultured on day 7. The cultures were maintained for up to six weeks.

Cell culture supernatant was collected for FeLV p27 antigen assay before feeding (3 to 5 days post-inoculation) and also 3 to 5 days post-subculturing. The collected supernatant then was tested for the presence of FeLV p27 by ELISA (Leukassay*F, Pitman Moore) according to the manufacturer's specified procedure. Cultures that gave two consecutive positive assays were considered "virus isolation positive"; cultures that remained negative for six weeks were considered "virus isolation negative".

The validity of this protocol was confirmed by testing supernatants for the presence of infectious retrovirus using the clone-81 virus assay described above in Example 1.

Results

At eleven weeks post-challenging none of the Group I cats were viremic by IFA or p27 ELISA assays. In contrast 4 of 6 Group II cats and 5 of 6 Group III cats were viremic by both assays. At post-challenge week five, 5 or 6 cats in Group II and 5 of 6 cats in Group III were virus isolation positive whereas 0 of 6 cats in Group I were virus isolation positive.

Virus was not isolated from the blood of the two cats that were positive by ELISA the day before challenge. Since virus isolation is generally a more sensitive test than ELISA, the ELISA results are considered to be spurious. It also is not known whether the failure of the vaccine produced from FeLV-A.sub.R2 grown in CRFK cells may be attributed to the effective dose, the characteristics of the antigen(s), and/or other factors.

In summary, the chronically infected ACFL line produced more virus and gp70 viral antigen than did the other cell line-virus isolate combinations studied. Virus from ACFL produced an efficacious vaccine, while virus from CRFK cells infected with a different isolate did not yield an efficacious vaccine. The ACFL line constitutes an improved system for producing high titer FeLV virus and the virus produced is useful in formulating vaccines. The ACFL virus also would be useful for production of diagnostic reagents.

These results are summarized below in Table 3.

All publications cited in this application are indicative of the level of skill of those skilled in the art to which this invention pertains. Each publication is individually incorporated herein by reference in the same location and to the same extent as if each publication had been individually incorporated by reference.

EXAMPLE 4-MULTIVALENT VACCINES

A four-way multivalent vaccine was prepared that included killed Felines leukemia Virus, killed Feline Rhinotracheitis Virus, killed Feline Calici Virus and killed Feline Panleukopenia Virus. In preparing the vaccine, that is an assembly of the serial, one or more lots each having inactivated virus, with each inactivated as described below were transferred to suitable sterile vessels. Adjuvant, as described below was added and the mixture was vigorously agitated for 60 minutes until the product was ready for fill. The components of the assembled product were as follows:

For this vaccine, the Feline Leukemia Virus employed was the Feline Leukemia Virus prepared as described in example 1 and inactivated as shown in example 3.

The FVR, FCV, and FPV viruses were grown in Eagles's Minimum Essential Medium (MEM) with the addition of up to 5% bovine serum. Lactalbumin hydrolysate (LAH) at 0.5% and gentamicin at 30 micrograms per milliliter were also added to the growth medium. The FVR, FCV, and FPV cells at a concentration of approximately 1.times.10.sup.5 cells per milliliter were suspended in the growth medium. The cultures incubated from two to seven days at approximately 36.degree. C. in roller bottles.

The FVR, FCV, and FPV viral fluids were harvested after the incubation period and immediately inactivated.

The virus inactivation of the FVR, FCV, and FPV was by use of binary ethyleneimine (BEI). Particular, BEI was added to the pooled viruses to achieve a final concentration of 0.005 molar BEI. The volume of BEI added was calculated by the following formula:

Inactivation was allowed to proceed at from 25.degree. C. to 36.degree. C. with mixing for a period of from one to three days.

After inactivation, the BEI is neutralized with Sodium Thiosulfate (Na.sub.2 S.sub.2 O.sub.3) Solution 50%. A sufficient volume of 50% Na.sub.2 S.sub.2 O.sub.3 is added to the virus to achieve a final molarity of 0.05M. The volume of Na.sub.2 S.sub.2 O.sub.3 added to neutralize the BEI is calculated by the formula:

In preparing the multivalent vaccine composition of the total vaccine included a preservative and an adjuvant. The preservative was a 10% merthiolate solution added to the inactivated virus to achieve a final concentration of 0.01%. The adjuvant was an oil and water emulsion containing a copolymer.

In testing for safety and efficacy of the multivalent vaccine, the vaccine as prepared above was administered to many cats in a 1.5 ml dose to each cat by intramuscular inoculation. It was repeated in three to five weeks. The cats were thereafter challenged with live FeLV, FVR, FCV, and FPV. Results of the challenge show that the cats were successfully immunized and the cats remained healthy. The vaccine, that is the multivalent vaccine, was thus shown to be an aid in preventing Viremia, leukemia-associated syndromes, and deaths caused by Feline Leukemia Virus, Feline Rhinotracheitis Virus, Feline Calici Virus, and Feline Panleukopenia Virus infections.

There was no evidence of incompatibility of each of the inactivated viruses in either stability of the total vaccine or from the standpoint of each interfering with development of the desire to antigenic response of the other. It can therefore be seen that the multivalent feline leukemia vaccine was successful for developing the desired immune response in cats.

Claims

What is claimed is:

1. A multivalent Feline Leukemia Virus vaccine comprising: an immunogenic response producing amount of inactivated Rickard isolate feline Leukemia Virus designated FeLV-A.sub.R1 ; an effective amount of at least one inactivated virus selected from the group consisting of Feline Rhinotracheitis Virus, Feline Calici Virus, and Feline Panleukopenia Virus; and a pharmaceutically acceptable immunologic adjuvant.

2. The multivalent vaccine of claim 1 in which the multivalent vaccine contains each of the following viruses: Feline Rhinotracheitis Virus, Feline Calici Virus, and Feline Panleukopenia Virus.

3. The multivalent vaccine of claim 1 wherein the amount of Feline Leukemia Virus is within the range of 6.5.times.10.sup.6 TCID.sub.50 to 1.times.10.sup.7 TCID.sub.50.

4. The multivalent vaccine of claim 1 wherein the amount of Feline Rhinotracheitis Virus is from about 10.sup.6.5 TCID.sub.50 to about 10.sup.7 TCID.sub.50.

5. The multivalent vaccine of claim 1 wherein the amount of Feline Calici Virus is from about 10.sup.5.6 TCID.sub.50 to about 10.sup.6 TCID.sub.50.

6. The multivalent vaccine of claim 1 wherein the amount of Feline Panleukopenia Virus is from about 10.sup.5.1 TCID.sub.50 to about 10.sup.6 TCID.sub.50.

7. The multivalent virus vaccine of claim 1 wherein the FeLV-A.sub.R1 is formalin inactivated.

8. The multivalent vaccine of claim 1 wherein the Feline Rhinotracheitis Virus, Feline Calici Virus and Feline Panleukopenia Virus have been inactivated with binary ethyleneimine.

9. A method of immunizing cats comprising administering to a cat a multivalent vaccine containing a) an immunologically effective amount of inactivated Rickard isolate Feline Leukemia Virus designated FeLV-A.sub.R1 in combination with b) an immunologically effective amount of at least one inactivated viral material selected from the group consisting of Feline Rhinotracheitis Virus, Feline Calici Virus, and Feline Panleukopenia Virus and c) a nontoxic pharmaceutically acceptable immunologic adjuvant.

10. The method of claim 9 wherein said multivalent vaccine is administered intramuscularly.

11. The method of claim 9 wherein said vaccine is administered subcutaneously.

12. The method of claim 9 wherein said vaccine is parenterally administered.

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