Dispersive Instrument for Measurement of Particle Size Distributions

This invention was made during the course of work performed under a contract with the Office of Naval Research. The invention pertains to the rapid measurement of the size distribution of particles suspended in a gas or in a liquid. For simplicity the discussion in the present section is made to refer to the case of water drops suspended in air. In most of the known instruments which are presently available for measurement of drop size distributions, sample air is moved through a small highly illuminated volume of space, and light scattered by the individual drops, as they move in a single file through this volume, is measured by a photodetector. The drop size is determined from the intensity of the electric pulse out of the photodetector. This method suffers from a number of deficiencies, notably the edge effect, the velocity effect and counter swamping. The edge effect arises because drops which pass through the scattering volume near the edge of the illuminating beam are illuminated less and therefore scatter less light. This causes the instrument to undersize the drop. The velocity effect arises in airborne applications, where the cloud drops move rapidly through the scattering volume. The resulting short detector pulse duration requires somewhat different electronics parameters than those used for terrestrial laboratory work, where the drop velocity is small. As a consequence, laboratory calibration of the instrument unless it is done in a windtunnel, is of limited utility for airborne measurements. Counter swamping may occur when drop size distributions are measured in dense clouds aboard a fast flying aircraft.

The measurement method comprised in the present invention suffers from none of the deficiencies discussed. Instead of counting and sizing individual scattering events, the instrument looks at a sizeable volume of cloud (from about one liter to several cubic meters) and it processes the light scattered from all drops in this volume collectively. This makes it possible to measure drop size distributions in clouds, fogs, or in laboratory cloud chambers in situ, without disturbing the cloud. Furthermore, it becomes possible to perform bulk measurements, in which average drop size distributions in a large volume are determined rapidly.

In practice, the light scattered by the multitude of individual particles adds incoherently. In the case of spherical particles, each particle scatters light in its characteristic Mie intensity pattern; these Mie patterns superimpose and give rise to a scattered light intensity which is a certain function of scattering angle. It is the object of the present invention to provide a means for determining the particle size distribution from this scattered light function.

Under circumstances often encountered, the conversion from the scattered light function to the particle size distribution constitutes an ill-conditioned data reduction problem, which results in low instrument accuracy. It is a further object of the present invention to circumvent this problem.

The measurement of the scattered light function may be done by means of an array of photodetectors, as in the apparatus of Malvern Instrument, Ltd., of Malvern Worcestershire, England. This requires the photodetectors to be small solid state devices, such as photodiodes, and this restricts the detectivity of the instrument such that only relatively dense particle suspensions can be measured accurately. Using photomultiplier tubes instead of photodiodes would result in a much increased instrument sensitivity, but use of any array of photomultiplier tubes would be prohibitive in cost and size. Therefore, there is merit in a method which requires only a single photodetector for the measurement of light scattered by the particles. For this photodetector, a photomultiplier tube can then be used.

It is a further object of the present invention to determine the particle size distribution by using a single photodetector for the measurement of the light scattered by the particles.

There are several situations in which it is desirable to utilize scattering angles near 180.degree., i.e., near backscattering. Such an arrangement makes it possible to have the light source and detector in the same general location, and yet measure particle size distributions in a large volume or at rather large distances from the apparatus. It is a further object of the present invention to provide a method for determining particle size distributions which can be used with near backscattered light.

Often, there is undesirable background light in the form of daylight, or from light sources extraneous to the instrument. In cloud chamber work, the illuminated cloud drops inside the chamber scatter light in all directions; some of this light is reflected by or scattered off the chamber walls, and this results in undesirable background light which enters the instrument. It is a further object of the present invention to provide means to effectively subtract background light in the instrument process.

It is yet another object of the present invention to provide a means of measuring particle size distributions in real time.

By way of summary, the objects of the invention are achieved by passing a collimated beam of white polarized light through the particle suspension and by collecting part of the light scattered by the particles located in a portion of the illuminating beam by a photodetector, after the light has passed through a lens and through a dispersive element, followed by a spatial filter placed in the exit plane of the dispersive element. Different spatial filters are brought into position behind the lens in quick succession, and the sequence of measured photodetector outputs is acted upon by a linear transformation, followed by the formation of a linear combination of basis functions for the particle size distribution, executed by a computer, microprocessor, or an analog device.

The invention will be fully understood from the following detailed description and the accompanying drawings, in which:

FIG. 1 shows schematically an instrument in which a disc is used for mounting and switching a plurality of spatial filters;

FIG. 2 shows schematically a modified instrument employing near backscattering, and a film strip for switching a plurality of spatial filters;

FIGS. 3-7 are plan views of transparencies bearing patterns which form spatial filters transmittance functions;

FIG. 8 shows the distribution of the perpendicularly polarized intensity of light scattered by water drops as a function of the scattering parameter and the scattering angle, illustrating the occurence of secondary correlation peaks; and

FIG. 9 shows an arrangement of the invention in which two lenses and a prism are used to make up the dispersive element, and in which a disc is used for mounting and switching of a plurality of spatial filters.

Referring now to FIG. 1, a light source 11 produces a collimated or nearly collimated beam 12 of nearly-monochromatic polarized light, which illuminates the particle suspension. A lens or lens system 13 processes light scattered by the particles located in the intersection 12b of the beam 12 and the cone 14, the latter constituting the field of view of an optical configuration consisting of lens 13 and an image window 15, located in the focal plane 16 of lens 13.

In this specification, the term "focal plane" is defined as the plane which sharply images objects at infinity.

A polarizing element 17 is placed in the path of scattered light, preferably but not necessarily between the lens 13 and image window 15. A perfect lens 13 would map incoming light rays located inside cone 14 onto points in image window 15. Therefore, part of the light scattered by an angle .theta. by all particles in the volume at intersection 12b is imaged onto a curve in window 15. As a consequence for spherical and monodispersed particles, the light intensity in image window 15 will be distributed according to a Mie intensity pattern.

A set of (N+1) spatial filters 18 is mounted on a disc 19 in plane 16. Arrangements are made such that, in time, different spatial filters are switched into the position of window 15.

An alternate embodiment of the switching concept consists of a filmstrip 19a, the frames of which are the individual spatial filters. The filmstrip is moved past the window in step-wise motionpicture fashion. The filmstrip (FIG. 2) can be looped, without beginning or end, if preferred.

There is the option of chopping the beam 12 of light by a chopper 22, and using synchronous detection in a manner which will be evident to those skilled in the art.

There is the further option of having either disc or filmstrip move continuously, and to flash the light source 11 synchronously with the filter position. A gated amplifier or integrator can then be used in the signal processing.

Each spatial filter consists of a transparency, the transmittance of which is a function of location on the transparency. Behind the image window 15 and the spatial filter 18 there is a photodetector 20, which is usually a photomultiplier tube. With the i.sup.th spatial filter in place, the signal from the photodetector 20, usually after amplification and integration, (possibly gated), is measured and serves as input s.sub.i for a computer 21. In the present context, the computer 21 may be a computer, a microprocessor, or an analog device, such as a CCD or CTD. There are N+1 different spatial filters on the disc 19, and hence, there are N+1 signals s.sub.i. The computer 21 is programmed or arranged such as to execute a linear transformation of the data sequence s.sub.i, i=0,1,-N, by means of a matrix T.sub.ij, which is present in the computer, or embodied in an analog device, in a manner as will be evident to those skilled in the art. The result of this linear transformation is the sequence ##EQU1## j=1,-n. As a further action of the computer, the sum ##EQU2## is computed, where the n.sub.j (a) are N basis functions for the particle size distribution function, a being the particle radius, or some other particle size characterizing number. The function n(a) is the particle size distribution which is the object of the measurement. It may be displayed, printed, or used as input for further calculations in a manner evident to those skilled in the art. The number b.sub.o is not used in the particle size distribution calculation (2), since things are arranged such that b.sub.o is a measure of the background light, and such that the b.sub.j, j=1,-N, are not influenced by the background light. Essential to this arrangement is the use of one spatial filter, say with j=0, which has uniform transmittance.

The action of the filters may be understood as follows. Suppose that the cloud consists of drops of a uniform size. If for that case the filter 18 in the image window 15 would be replaced by a screen, the light processed by the apparatus would illuminate this screen with a non uniform intensity, showing light and dark bands, which are called a Mie pattern. The Mie pattern depends on the scattering angle range in the field of view of the cone 14, and it also depends on the size and the refractive index of the scattering cloud drops. If now different filters are switched into place, maximum light would get passed by a filter which has a transmittance pattern which precisely matches the MIE pattern of the incoming light. In this manner, the Mie pattern of the light coming into the window 15 is recognized, and the drop size is inferred. For a cloud with a mixture of drop sizes, the algorithym discussed unravels the separate contributions by the Mie patterns belonging to the different drop sizes to the total light intensity distribution in the window. It is possible to use filter transmittance distributions other than Mie patterns belonging to single drop sizes, by taking linear combinations of such Mie patterns for different sizes.

In order to enable those skilled in the art to construct the proper transmittance functions for the spatial filters 18, the following discussion is presented, in which square integrable functions are considered as vectors in a Hilbert space H. Let n denote the vector of the particle size distribution n(a), and let v denote the vector of the light intensity distribution of the scattered light incident on the image window 15. Since in practical cases the light scattered by the individual particles adds incoherently, one has

where M is a linear operator on H, which may be thought of as an infinite dimensional matrix. If M is non singular, one has

where M.sup.-1 is the inverse of M. In practice, measurement of v contains noise .DELTA.v, which results in a noise part

in the measured particle size distribution. From (4) and (5) one has ##EQU3## where .parallel. denotes the Euclidean norm (see, for instance, W. Schmeidle "Linear Operators in Hilbert Space", Academic Press, New York, 1965, pages 10 and 13). Therefore, the inverse map (4) brings about a signal to noise ratio degradation ##EQU4## where the maximum is taken over non-negative functions n, and the minimum is taken over functions .DELTA.n which have no definiteness restriction. In (6), the vectors n and .DELTA.n over which the maximum and minimum are taken may be restricted to a subspace S of H. Then, .DELTA. depends on the subspace S, and, of course, on the scattering angle interval comprized by the field of view cone 14. If .beta..sub.0 is an acceptable value for the degradation of the inverse map (4), a subspace S of H is called good if .beta..ltoreq..beta..sub.0. The data reduction method outlined amounts to approximating particle size distributions as vectors in a good subspace G. The utility of this procedure depends on whether in practice particle distribution and their differences have negligible components in the orthogonal compliment B of G. Calculations have shown that for waterdrops in air, and for scattering angle ranges away from near-forward or near backward directions, the subspace B contains vectors .DELTA.n which can occur in practice as the difference between broad polydispersed drop size distributions. This is because in the Mie intensity pattern for monodispersed waterdrops in air the maxima and minima in the region away from the near forward and near backward directions shift very rapidly with changing scattering parameter .alpha.=2.pi.a/.lambda. (a is the drop radius, and .lambda. is the light wavelength), whereas the distance between consecutive maxima varies slowly with .alpha.. Hence, if one adds the Mie patterns of two drops of slightly different sizes, the maxima and minima fill in, thereby diminishing the norm of the ac component of v. It follows that an instrument designed for a scattering angle range away from the near-forward or near-backward directions is only suitable to measure rather monodisperse drop size distributions. For accurate measurement of broad polydisperse drop size distribution one must use near-forward or near backward scattering as in FIG. 2. This is because for varying .alpha. the scattering intensity versus scattering angle .theta. always has a maximum at .theta.=0, and either a maximum or a minimum at .theta.=180.degree.. Therefore, the "phase" of the Mie "waves" is locked in at .theta.=0.degree., and at .theta.=180.degree. (up to a flip of .pi.), so that, near these angles, the maxima and minima do not change position so rapidly as function of .alpha.. Furthermore, comput studies have shown that the near-backscatter range is more suitable for the accurate measurement of broad polydispersed drop size distributions, if Gaussians with a relative standard deviation of about 10% are used as basis functions, in a manner described below. Let the N linearly independent basis functions n.sub.i, i=1,-N, belong to the good subspace G. We approximate the particle size distribution as a linear combination ##EQU5## With

one has ##EQU6## A solution for b.sub.i may be obtained by forming the scalar products (correlations) ##EQU7## where

One then has ##EQU8## where C.sub.ij.sup.-1 is the inverse of C.sub.ij. The particle size distribution is found from ##EQU9## The correlation v.multidot.v.sub.j is implemented in the present invention by passing the scattered light through lens 13 and through the j.sup.th spatial filter 18 with transmittance V.sub.j in focal plane 16 of lens 13. The light incident on the filter has the intensity distribution v. The total light intensity transmitted by the filter is v.multidot.v.sub.j. C.sub.ij.sup.-1 is essentially the instrument matrix T.sub.ij of (1); further discussion clarifying the role of i=0 or j=0 follows.

Since the basis functions n.sub.j lie in the good subspace G, they can be chosen such that the matrix is far from singular, and the matrix multiplication (13) does not give rise to a large degradation of signal to noise ratio. (12) written in the form ##EQU10## shows that the linear transformation can be absorbed in the optical process, by taking spatial filters with transmittance functions ##EQU11## Resulting negative transmittance can be embodied in practice by using a bias to raise all filter transmittances to nonnegative values, and by adding an (N+1) st filter (J=0) with a uniform transmittance. This filter can also be used to achieve subtraction of background light, by considering the uniform function n.sub.o as the density of a "dummy" scattering object, which gives a scattering function v.sub.o equal to the background light contribution to v. Including the dummy scatterer in (7) gives ##EQU12## and extending the range of j in (12) gives ##EQU13## where now, C.sub.ij.sup.-1 is the inverse of the N+1 dimensional matrix C.sub.ij. The bi,i=1,-N, of (16) are not influenced by the background light. The inclusion of the linear transformation in the optical process, and the biasing of the filter transmittances to non-negative values may be done as follows. Inclusion of j=0 in (14) gives ##EQU14## Let C.sub.i be a positive number such that none of the functions ##EQU15## attains negative values anywhere on the window 15. Then, with a.sub.i =max g.sub.i, the functions g.sub.i,i=1,-N, are implementable as transmittance functions of the filters i=1,-N, (17) may be written

If s.sub.i is the photodetector signal with the ith filter in position. (19) may be written

Hence, this embodiment gives a shorter computer calculation of the coefficients b.sub.i of expansion of the particle size distribution .eta.(a) in terms of the basis functions n.sub.i (a), i=1,-N.

It will be evident to those skilled in the art how to apply this procedure to the case in which the background light illuminates window 15 in a non-uniform manner, and a 0th filter with an appropriate non-uniform transmittance is used for the implementation of indefinite filter transmittances. The description above has been given in terms of water drops in air. It applies as well to other spherically symmetric particles, suspended in a gas or a liquid except that certain ratios of refractive index of the particles and the suspending medium may favor near forward scattering rather than near backscattering. In such cases, the scattering angle range must be appropriately adjusted, but the method of measurement and date reduction remains the same. The method also applies to the case of nonspherically symmetric particles suspended in a gas or liquid, possibly with some degradation of performance of the instrument. The particles may be biological cells, bacteria, or other biological organisms, and the invention may be used to determine the concentrations of the various species of cells, bacteria, organisms, or particles which are present in a sample. The particles may be suspended on a transparent slide which is illuminated by the light beam, instead of being suspended by a gas or a liquid in the bulk.

There are various options concerning the polarization of the beam 12, and the polarizing element 17, as follows. The instrument will work with an unpolarized light source 11, and without the polarizing element 17. In fact, this is the preferred polarization mode for very near forward scattering or very near backscattering, i.e., where the intensities of the scattered light in both polarized directions are substantially the same. For scattering angles not very close to 0.degree. or 180.degree., an improvement in instrument performance is achieved by using a light source 11 which is polarized. (E direction) perpendicular to the plane P through the illuminating beam axis and the optical axis of the lens. The use of a polarizing element 17 is then optional; if used, the polarization direction should be perpendicular to the plane P. As an alternative, an unpolarized light source 11 can be used, together with a polarization element 17, polarized perpendicular to the plane P. As a further alternative, the light source 11 may be polarized in the direction of P, and there is then again the option of using a polarizing element 17; if used, the element 17 should be polarized in the direction of P. As a further alternative, an unpolarized light source may be used, with element 17 polarized in the direction of P.

A modified instrument which is particularly suitable for use in clouds of fog is shown schematically in FIG. 2. In this case, near backscattering is used, making it possible to have light source 11 and detector unit close together while sampling a substantial volume of cloud or fog. The element numbers are the same as in FIG. 1, with the exception of the embodiment of the set of filters, which now is in the form of a filmstrip 19a looped and therefore without end. It is important that either the illuminating light intensity in beam 12 is very nearly the same from frame to frame exposure of the filmstrip, or else that the variation of this light intensity is measured accurately. It will be evident to those skilled in the art how to achieve this objective, if necessary by the use of an auxiliary photodetector which measures the illuminating light intensity.

FIGS. 3-7 show examples of negatives of transmittance functions for spatial filters for use in FIG. 1 with scattering angle near 90.degree.. These functions have been generated by a computer-driven plotter, from a calculation of Mie intensities for water drops in air. In these plots, the dot density along vertical lines is proportional to the average filter transmittance along vertical lines. In this particular example, the average scattering angle is 90.degree., and the coarse-grained average filter transmittance is approximately constant along vertical lines. For average scattering angles different from 90.degree., the filter transmittance is constant along curves which are conical sections of constant scattering angle .theta..

Another method for the construction of the filters consists of making a picture in the form of a slide, of a semi-transparent sheet assembly, which is illuminated from the opposite side of the camera, where the sheet assembly consists of strips of semi-transparent material such as paper, but together in layers in such a manner as to produce an assembly with the desired transmittance pattern.

Yet another method for the construction of the filters consists of producing a cloud with uniform drop size, or with a certain desired drop size distribution, and using a camera instead of the assembly 13, 18, 19 and 20, in such a manner that the camera lens is identical to the lens 13, and is placed in the position of lens 13 and such that the camera film window coincides with the image window 15. Using the proper exposure time, the picture produced in this manner, in the form of a slide, will have the proper transmittance pattern. A preferred method for the construction of the filters consists of controlling a camera orientation by means of two computer-driven angular positioners, and illuminating the camera lens with a fixed collimated light beam, which passes through a computer-controlled shutter. Alternatively, the intensity of the light beam may be modulated by the computer.

When a polarizing element is placed in the path of the scattered light, it may be placed on either side of the lens, preferably ahead of the spatial filter.

Chopping or flashing the light source is useful but not essential. A steady light source can be used. However, significant advantages are obtained by providing light intermittently, for example improvement in the signal to noise ratio, discrimination against background light, and discrimination against dc output voltage drift of the amplifiers behind the photodetector.

The accuracy of instruments of the type discussed above may be further improved by using multiple-wavelength light and by including a dispersive element in the detector optics, in a manner as described below.

Eq. (8) may be written

where a is the particle radius, n.sub.i (a) is the particle size distribution for the ith basis function, a, and a.sub.2 are respectively the smallest and largest particle radii in the size distribution n.sub.i (a), M(.theta.,.alpha.) is the differential scattering cross section, and .alpha. is the scattering parameter defined before,

where .lambda. is the wavelength of the monochromatic light used. With (21), Eq. (11) may be written

where .theta..sub.1 and .theta..sub.2 are respectively the smallest and largest scattering angles for the light processed by the instrument optics, and

Eq. (23) may be rewritten as

where

is seen to serve as a metric in the Hilbert space of square integrable particle size distributions .eta.(a); in fact, (25) may be seen as the scalar product n.sub.i .multidot.n.sub.j in that Hilbert space, using the metric (26).

The data reduction algorithm (13) involves the inverse C.sub.ij.sup.-1 of the matrix C.sub.ij. Therefore, the degradation of the signal to noise ratio in the data reduction will depend on the condition number of the matrix C.sub.ij (defined as the ratio of maximum to minimum absolute values of the eigenvalues). In turn, this condition number is influenced by the condition number of the metric g(a,a'). Eq. (26) shows that this metric is just the correlation (over the interval .theta..sub.1,.theta..sub.2) of the differential scattering cross sections M(.theta.,.alpha.) and M(.theta.,.alpha.'). This correlation has a peak for .alpha.'=.alpha., but there exist secondary correlation peaks as well for certain values of .alpha. and .alpha.'. This may be seen from FIG. 8, which shows the differential cross section M(.theta.,.alpha.) for the perpendicularly polarized component of light scattered by water drops. In this figure the symbol A is used for the scattering parameter, instead of .alpha.. The Mie function M(.theta.,A) is plotted in the form of contour lines as function of the scattering paramter A and the scattering angle .theta.; the numerical values for the contours shown are ##EQU16## where i.sub.1 is the perpendicularly polarized scattered light intensity. In this case, one has .theta..sub.1 =145.degree. and .theta..sub.2 =152.degree.. FIG. 8 may be seen as a topograhic map of terrain with mountains, lakes, valleys, ridges, and canyons. It may be seen that the ridge-like mountain at about A=11.4 is quite similar to the ridge-like mountain at about A=16.3; this similarity extends at least over an interval .DELTA.A.apprxeq.1 to the right. It follows that the correlation (26) has a secondary peak for .alpha. in the neighborhood of 11.5 and .alpha.-.alpha.'.apprxeq.4.8. Such secondary correlation peaks adversely affect the condition number of the metric g(a,a'), and thereby diminish the instrument accuracy.

This problem may be alleviated to a considerable extent by using light with a plurality of different wavelengths such that the optical processing involves the sum or integral of correlations at different wavelengths .lambda..

For that case, the sequence of Eqs. (8) to (13) becomes ##EQU17## similar modifications, amounting to replacing the scalar product .cndot. by the "color" scalar product .cndot. , apply to the remaining equations, (14) to (20). Eq. (10') shows that the color scalar product .cndot. is simply the integral, over the wavelengths from .lambda., to .lambda..sub.2, of the ordinary scalar product .cndot. in Hilbert space. If for the transmittance function for wavelength .lambda. of the spatial filter j one chooses the function v.sub.j (.lambda.), then the correlation v .cndot. v.sub.j occuring in (13') is implemented by the spatial filter action. For the multiple-wavelength case one has, instead of Eqs. (21) to (26), ##EQU18## The relief from strong secondary correlation peaks provided by the wavelength integral in (26') is now evident: for a and a'.noteq.a such that there is a strong correlation in one wavelength, there is usually weak correlation in other wavelengths; hence, in the wavelength integral (26'), the secondary correlation peaks are diminished.

The implementation of the multiple-wavelength scheme may be accomplished as follows. Referring to FIG. 9, a light source 400 produces a collimated or nearby collimated beam 401 of substantially white light, which illuminates the particle suspension. The beam 401 has an axis 401a. A lens or lens system 402 processes light scattered by the particles located in the intersection 403 of the beam 401 and the cone 404, the latter constituting the field of view of an optical configuration consisting of lens 402, and a slit 405 in an opaque screen 406, located in the focal plane of lens 402. The slit 405 is oriented such that it is substantially parallel to the scattering plane for a processed scattered light ray which runs along the optical axis 402a of the lens 402; this scattering plane is substantially parallel to the optical axis 402a and the beam axis 401a. A polarizing element 407 is placed in the path of the scattered light, preferably but not necessarily between the lens 402 and the slit 405.

The scattered light which is transmitted by the slit 405 is made to pass through a dispersive element consisting of a lens 408, a prism or set of prisms 409, and a lens 410, in such a manner that the slit 405 is in the focal plane of lens 408, and focussed spectral decomposition of the processed light is obtained in the exit plane 411, which is the focal plane of lens 410. The prism orientation is such that the prism generators are parallel to the slit 405.

A set of spatial filters 412 is mounted on a disc 413 in plane 411. Arrangements are made such that in time, different spatial filters are switched in the position of window 414, located in plane 411.

An alternate embodiment of the switching concept consists of a filmstrip, the frames of which are the individual spatial filters. The filmstrip is moved past the window 414, in step-wise motion-picture fashion. The filmstrip can be looped, without beginning or end, if preferred.

The spatial filters 412 of FIG. 9 are black and white transparencies with certain spatial transmittance functions of the coordinates x and y in the plane 411.

There is the option of chopping the beam 401, and using synchronous detection in a manner which will be evident to those skilled in the art; the chopping may be done mechanically or by switching of the light source 400.

There is the further option of having either disc or filmstrip move continuously, and to flash the light source 400 synchronously with the filter position. A gated amplifier or integrator can then be used in the signal processing.

Behind the image window 414 and the spatial filter 412 there is a photodetector 415, which is usually a photomultiplier tube. With the ith spatial filter in place, the signal from the photodetector 415, usually after amplification and integration, (possibly gated), is measured and serves as input s.sub.i for a computer 416, which may be a computer, a microprocessor, or an analog device, such as a CCD, or CTD. There are N+1 different spatial filters on the disc 413, and hence, there are N+1 signals s.sub.i. The computer 416 is programmed such as to execute the algorithm (13'), where the matrix C.sub.ij.sup.-1 is the inverse of the matrix C.sub.ij given by (11'). Background subtraction may be achieved by color-generalizing Eq. (16), and biasing the filter transmittances to non-negative values may be achieved by color-generalizing Eqs. (17), (18), (19), and (20), in a manner which will be evident to those skilled in the art. The considerations concerning polarization, as discussed above for the monochromatic instrument, apply without change to the multiple-wavelength instrument. The same comment applies to the consideration pertaining to the illuminating light intensities for the different frames or spatial filters.

The dispersive element, shown in FIG. 9 to consist of the lens 408, the prism or prisms 409, and the lens 410, may be implemented differently by means involving a grating or hologram in a manner evident to those skilled in the art.

All the methods for construction of the spatial filters which are discussed above for the single wavelength case may be used for the multiple-wavelength instrument as well. The preferred method for the construction of the spatial filters consists again of controlling a camera orientation by means of two computer-driven angular positioners, and by illuminating the camera lens with a fixed collimated light beam, which either passes through a computer-controlled shutter, or else, has an intensity modulated by the computer.

This invention is not to be limited by the embodiments shown in the drawings and described in the description, which are given by way of example and not of limitation, but only in accordance with the scope of the appended claims.