Methods for the Preparation of Semi-Synthetic Aminocyclitol Aminoglycoside Antibiotics

1. A process for producing Antibiotics Mu-1, Mu-2, Mu-4, Mu-5 and Mu-6 which comprises fermenting Micromonospora inyoensis strain 1550F-1G in an aqueous nutrient medium, adding to the fermentation a compound of the formula: ##STR16## wherein A is a member selected from the group consisting of hydrogen, amino, hydroxy and (C.sub.1 -C.sub.8) alkoxy, B is a member selected from the group consisting of hydrogen and hydroxy, wherein the wavy lines connecting A and B to the ring nucleus denote that such groups may be in any of the possible stereoisomeric forms, with the proviso that when B is hydrogen and A is hydroxy, A must be cis to the hydroxyl groups adjacent thereto and with the further proviso that when A is amino, it must be trans to the hydroxy groups adjacent thereto, continuing the fermentation until a composition of matter having substantial antibacterial activity is produced and isolating an antibiotic therefrom.

2. A process according to claim 1 wherein the fermentation is conducted at from about pH 6.0 to about pH 8.0 for about 2 to about 10 days.

3. A process according to claim 2 wherein the fermentation is conducted at from about 24.degree. to about 49.degree. C.

4. A process according to claim 3 wherein the fermentation is conducted at about 35.degree. C for about 7 days from about pH 6.8 to about pH 7.4.

5. The process according to claim 4 wherein the compound added to the fermentation medium is streptamine thereby producing Antibiotic Mu-1.

6. The process according to claim 4 wherein the compound added to the fermentation medium is 2,5-dideoxystreptamine thereby producing Antibiotic Mu-2.

7. The process according to claim 4 wherein the compound added to the fermentation medium is 2-epi streptamine thereby producing Antibiotic Mu-4.

8. The process according to claim 4 wherein the compound added to the fermentation medium is 1,3,5-triaminocyclohexane-4,6-diol thereby producing Antibiotic Mu-5.

9. The process according to claim 4 wherein the compound added to the fermentation medium is 5-epi-2-deoxystreptamine thereby producing 5-epi-sisomicin.