Process for Preparing Pyridine Derivatives

The invention relates to a new process for preparing pyridine derivatives.

The invention provides a process for preparing compounds of formula I ##STR1## and acid addition salts thereof, wherein Z is CONH.sub.2, CSNH.sub.2 or CN, R.sup.1, R.sup.2 and R.sup.6 are the same or different and represent hydrogen, trifluoromethyl, or an alkyl, aralkyl or aryl radical, any of which radicals may be substituted by alkyl, nitro or trifluoromethyl or R.sup.1 and R.sup.2 taken together represent an alkylene chain --CH.sub.2 (CH.sub.2).sub.n CH.sub.2 -- wherein n is 1, 2 or 3, R.sup.7 represents single or multiple substitution by hydrogen, or alkyl, aralkyl or aryl radicals any of which radicals may be substituted by alkyl, nitro or trifluoromethyl and when R.sup.1 and R.sup.2 taken together form an alkylene chain the resulting ring may be substituted by one or more R.sup.7 radicals as defined above, and m is 1, 2 or 3, which process comprises treating a compound of formula II ##STR2## wherein R.sup.1, R.sup.2, R.sup.6, R.sup.7 and m are as defined in connection with formula I above and M is sodium, potassium, lithium or MgHal where Hal is chlorine, bromine or iodine, with a silyl compound of formula R.sub.x Si(NCY).sub.4-x wherein R is an alkyl, aryl or aralkyl residue and R.sub.x may be any mixture of these, Y is oxygen or sulphur and x has a value from 0 to 3 then subjecting the product to hydrolysis or alcoholysis, with the proviso that when a compound of formula I in which Z is CN is desired the molar ratio of compound R.sub.x Si(NCY).sub.4-x to compound IIa is at least 2:1 and x is 3 and Y is S.

Compounds of formula I in which Z is CSNH.sub.2 or CN display pharmacological activity, namely anti-ulcer and/or antisecretory activity. [Compounds where Z is CONH.sub.2 are intermediates.]

Examples of the compound R.sub.x Si(NCY).sub.4-x are:

wherein R has any of the meanings given above.

When x is 3 the residue R.sub.x Si may be a tri-alkyl-, tri-aryl- or tri-aralkyl-silyl group and is preferably a tri-lower alkyl silyl group, e.g. trimethylsilyl.

The reaction with the compound of formula R.sub.x Si(NCY).sub.4-x is conducted under anhydrous conditions, preferably in an inert solvent, for example a hydrocarbon solvent such as benzene, toluene or hexane. Ethers including cyclic ethers such as tetrahydrofuran should be avoided. Conveniently the starting material of formula II is prepared in situ and the same solvent is used for the reaction with the compound of formula R.sub.x Si(NCY).sub.4-x. However, where a compound of formula II wherein Z is MgHal is used these are usually prepared in ether as solvent. The ether is removed and the reaction with the silicon compound is conducted in a different solvent.

The product of the first stage is a compound of formula III ##STR3## (wherein R.sup.1, R.sup.2, R.sup.6, R.sup.7 and m are as defined in connection with formula I and Y, M, R and x are as defined above) which is converted by water or alcohol to the desired compound of formula I, presumably via a transient intermediate of formula IV ##STR4## wherein R is the organic residue above and Y is oxygen or sulphur and M, R.sup.1, R.sup.2, R.sup.6, R.sup.7, m and x are as defined in connection with formula II above.

Compounds of formula III and IV are included in the present invention as is the process for preparing them described above. These compounds are not isolated but are obtained and used in solution.

The desired compound of formula I wherein Z is CONH.sub.2, or CSNH.sub.2 is conveniently formed by treating a compound of formula III with water or a lower alcohol, e.g. ethanol.

The starting materials of formula II wherein M is sodium potassium or lithium are described in German Offenlegungsschrift 2,352,585 or may be prepared by analogous methods. They may be prepared by reacting a compound of formula I wherein Z is hydrogen with a metal alkyl e.g. MR.sup.10 wherein M is sodium, potassium or lithium and R.sup.10 is alkyl, aryl or aralkyl.

It has been found that when a compound of formula I in which R.sup.1 is methyl and R.sup.2, R.sup.6 and R.sup.7 are hydrogen and M is hydrogen is treated with metal alkyl the metal atom may be inserted either at the desired position or in the methyl group R.sup.1. This side reaction may also occur with any compound containing an alkyl group R.sup.1 in which there are one or two hydrogen atoms on the carbon atom adjacent to the pyridine ring. These byproducts which contain a metal atom in an alkyl group R.sup.1 do not normally react with alkyl-silyl isothiocyanates.

Compounds of formula II where M is MgHal may be prepared by treating a compound of formula I wherein Z is hydrogen with an alkyl magnesium halide R.sup.11 MgHal wherein R.sup.11 is an alkyl group, preferably a lower akyl group, and Hal is chlorine, bromine or iodine. R.sup.11 may be a straight or branched chain alkyl group, the isopropyl group being preferred. Conveniently the compound of formula II is prepared in situ. If ether is used as a solvent this is distilled off and another solvent added after which the product is then treated with the compound of formula R.sub.x Si(NCY).sub.4-x, followed by hydrolysis or alcoholysis to obtain the desired compound of formula I.

When any of R.sup.1, R.sup.2, R.sup.6 or R.sup.7 is an alkyl radical it is preferred that this is a lower alkyl radical which may be a straight or branched chain, having from 1 to 6 carbon atoms, e.g. methyl, ethyl, n-, and iso-propyl and n-, s- and t-butyl, R.sup.7 may be a gem-dimethyl group and when a single radical may be on the same carbon atom as the group Z. The term alkyl radical is also intended to embrace cyclic alkyl radicals e.g. cyclobutyl, cyclopentyl and cyclohexyl. When any of R.sup.1, R.sup.2, R.sup.6 or R.sup.7 is an aralkyl radical it is preferred that this is an aryl-lower alkyl radical where the lower alkyl portion may be as discussed above for a lower alkyl radical. The aryl portion is preferably a phenyl radical.

When any of R.sup.1, R.sup.2, R.sup.6 or R.sup.7 is an aryl radical, this is preferably phenyl or a substituted phenyl radical (substituted by alkyl, nitro or trifluoromethyl). However, other aryl radicals which may be used include naphthyl.

Particularly preferred compounds are bicyclic compounds especially those in which one of R.sup.1, R.sup.2 and R.sup.6 is methyl, the others are hydrogen and R.sup.7 is hydrogen. Tricyclic compounds may be symmetrical (i.e. n and m are equal) or unsymmetrical i.e. n and m are different. (i.e.

Also preferred are compounds where m is 2.

We have found that by conducting the above reaction with a compound (II) and a compound of formula R.sub.3 SiNCS wherein the molar ratio exceeds 2:1 a compound of formula I wherein Z is cyano is obtained in addition to the compound of formula I wherein Z is CSNH.sub.2.

For the preparation of compounds where Z is CSNH.sub.2 or CONH.sub.2 it is preferred that the ratio of the silyl compound to the compound of formula II is in the range 0.5:1 to 2:1, e.g. 0.5:1 to 1.5.

With higher ratios e.g. 4:1 the cyano compound has been obtained exclusively in certain instances.

Accordingly the present invention provides in one aspect a process for preparing a compound of formula I as defined above wherein Z is cyano which comprises treating a compound of formula (II) as defined above with a compound of formula R.sub.3 SiNCS in at least 2:1 molar ratio, wherein R is an alkyl, aryl or aralkyl residue or R.sub.x is any mixture of these and subjecting the product to hydrolysis or alcoholysis.

We have also found that yields of the final product of formula I wherein Z is CONH.sub.2 or CSNH.sub.2 may be improved if the reaction with the metal alkyl is conducted in the presence of a secondary amine (preferably in a molar amount equal to that of the metal alkyl) and then followed by reaction with the silyl isothiocyanate or isocyanate. This may be achieved conveniently by preparing the starting material of formula II wherein M is lithium sodium or potassium in situ by reaction of a compound of formula II wherein M is hydrogen with a metal amide derived from a secondary amine.

Accordingly the present invention provides in another aspect a process for preparing a compound of formula I, as defined above, in which a corresponding compound of formula I wherein Z is hydrogen is treated with a metal amide MA wherein M is sodium, potassium or lithium and A is a secondary amine radical, and then treating the product with a compound of formula R.sub.x Si(NCY).sub.4-x as defined above and then subjecting the product to hydrolysis or alcoholysis.

The metal amide may be formed in situ by reacting a metal MR.sup.10 wherein M is sodium, potassium or lithium and R.sup.10 is alkyl, aryl or aralkyl with a secondary amine, (preferably in a molar amount equal to that of the metal alkyl). The compound of formula I wherein X is hydrogen may then be added.

Preferably the metal M is lithium. The secondary amine may be a dialkylamine e.g. diethylamine, di-isopropylamine, di-tertiarybutyl amine, di-n-decylamine, dicyclohexylamine, N-tertiaryamyl-N-t-butylamine, N-isopropyl-N-cyclohexylamine, or N(1'-ethylcyclohexyl)-1,1,3,3,tetramethylbutylamine, or a cyclic compound e.g. piperidine, or 2,2,6,6-tetramethylpiperidine.

The following examples illustrate the invention. Temperatures are in .degree.C.

A solution of 3-methyl-5,6,7,8-tetrahydroquinoline (7.3 g., 0.05 mol.) in hexane (50 ml.) was cooled to 0.degree. C and treated dropwise with stirring with a 15% w/w solution of butyl lithium in hexane (26 ml., 0.06 mol.) in an atmosphere of nitrogen. The reaction mixture was stirred at 0.degree. C for an additional 1 hour and was added portionwise to a solution of trimethylsilylisothiocyanate (13.1 g., 0.1 mol.) in hexane (50 ml.) and under nitrogen keeping the internal temperature at -20.degree. C. The reaction mixture was stirred for an additional 30 minutes at -20.degree. C, allowed to warm to room temperature and diluted with water (50 ml.). The pH was adjusted to 2.0 with conc. HCl and the hexane layer separated and discarded. The aqueous solution was adjusted to pH 10.0 with sodium carbonate and extracted with ethyl acetate (3 .times. 50 ml.). The combined extracts were dried (MgSO.sub.4) and the solvent removed in vacuo. The residue was triturated with n-hexane and solid filtered and recrystallised from benzene to give the title compound as colourless needles (3.5 g., 39%) m.p. 153.degree. identical in all respects to authentic material. (Found: C, 64.6; H, 7.0; N, 13.9%. C.sub.11 H.sub.14 N.sub.2 S Requires: C, 64.1; H, 6.8; N, 13.6%). The hexane soluble material was distilled to give recovered 3-methyl-5,6,7,8-tetrahydroquinoline (3.5 g., 50%) b.p. 120.degree./15 mm.

A solution of 3-methyl-5,6,7,8-tetrahydroquinoline (7.3 g., 0.05 mol.) in hexane (50 ml.) was cooled to 0.degree. C and treated dropwise with 15w/w butyllithium in hexane (26 ml., 0.06 mol.) in a nitrogen atmosphere. The reaction mixture was allowed to stand at 0.degree. C for 1 hour and was then added dropwise over 30 minutes to a solution of trimethylsilylisocyanate (19.5 g., 0.17 mol.) in hexane (50 ml.) keeping the internal temperature at -20.degree. C. The reaction mixture was allowed to stand at -20.degree. C for 1 hour and was then diluted with water (50 ml.) and the pH adjusted to 2.0 with conc. HCl. The hexane layer was separated and discarded and the aqueous solution adjusted to pH 10.0 with sodium carbonate and extracted with chloroform (3 .times. 25 ml.). The combined extracts were washed with saturated brine, dried and the solvent removed in vacuo. The residue was triturated with n-hexane, filtered and the solid crystallised from ethyl acetate to give the title compound as colourless needles (3.1 g., 35%) m.p. 104.degree. C. identical in all respects to the authentic material (when crystallised from ethyl acetate). Analysis: Found C, 69.1; 7.4; N, 14.7. C.sub.11 H.sub.14 N.sub.2 O requires C, 69.5; H, 7.4; N, 14.7. The hexane soluble material was distilled to give recovered 3-methyl-5,6,7,8-tetrahydroquinoline (4.2 g.) b.p. 120.degree./15 mm.

A solution of 3-methyl-5,6,7,8-tetrahydroquinoline (29 g., 0.2 mol) in benzene (200 ml.) was cooled to 0.degree. C and treated dropwise with a 15% w/w solution of n-butyl lithium in hexane (88 ml., 0.2 mol) under nitrogen. After 1 hour at 0.degree. C the reaction mixture was added portionwise to a solution of trimethylsilylisothiocyanate (112 ml., 0.8 mol.) in benzene (200 ml.) at 0.degree. C and under nitrogen. After 21/2 hours at room temperature the reaction mixture was treated with water (100 ml.) and with 2N HCl to pH 2.0. The aqueous layer was separated, washed with ethyl acetate (1 .times. 50 ml.) and the pH adjusted to 9.0 with sodium carbonate and extracted with chloroform (3 .times. 100 ml.) The combined chloroform extracts were dried (MgSO.sub.4) and the solvent removed in vacuo to give a red oil which was distilled first at 0.25 mmHg to give recovered 3-methyl-5,6,7,8-tetrahydroquinoline (17 g., 59%) b.p. 54-7.degree. and then at 0.05 mmHg to give the title compound as a pale red oil (12 g. 35%) b.p. 115- 20.degree. R.sub.T = 4.1/4 min. (3% SE30, 200.degree. C) identical to authentic material.

A solution of 4-methyl-5,6,7,8-tetrahydroquinoline (5.83 g., 0.04 mole) in dry benzene (40 ml.) was cooled to 0.degree. and to the stirred solution was added dropwise a 15% w/w solution of butyl lithium in hexane (17.5 ml., 0.04 mole) under an atmosphere of nitrogen. The red reaction mixture was stirred at 0.degree. for a further 30 minutes. Trimethylsilylisothiocyanate (5.6 ml., 0.04 mole) was then added dropwise, maintaining the temperature at 0.degree.. After an additional 30 minutes, the mixture was allowed to warm to room temperature and diluted with water (40 ml.) The pH was adjusted to 2.0 by addition of conc. HCl and the benzene layer separated and discarded. The aqueous phase was adjusted to pH 10.0 by adding anhydrous Na.sub.2 CO.sub.3 and extracted with CHCl.sub.3 (3 .times. 40 ml.). The CHCl.sub.3 solution was then dried (MgSO.sub.4), filtered and evaporated (reduced pressure) to afford an oil (5.77 g.). Addition of ether caused crystallisation of the title compound as colourless needles. Filtration afforded 0.69 g. of base which was converted to the hydrochloride by dissolving in a minimum of EtOH, adding EtOH/HCl until just acid followed by ether to induce crystallisation. Filtration provided the title compound hydrochloride as colourless needles, (0.64 g.), m.p. 213.degree. C. Analysis: Found: C, 54.95; H, 6.40; N, 11.52 C.sub.11 H.sub.14 N.sub.2 S.HCl requires C, 54.42; H, 6.23; N, 11.54%

A solution of 3,7,7,-trimethyl-5,6,7,8-tetrahydroquinoline (10.3 g. 59 m. mole) in hexane (50 ml.) was cooled to 0.degree. C and treated dropwise with a 15% w/w solution of butyl lithium in hexane (25.8 ml., 59 m. mole) and allowed to stand at 0.degree. for 1 hour. The solution was treated dropwise with trimethylsilylisothiocyanate (8.25 ml., 59 m. mole) in hexane (50 ml.) and allowed to stand at 0.degree. C for 11/2 hour. The reaction mixture was warmed to room temperature diluted with water (25 ml.) and the pH adjusted to 1.0 with conc. HCl. The solution was extracted with ether (3 .times. 25 ml.) and the aqueous phase adjusted to pH 10.0 with sodium carbonate and extracted with chloroform (3 .times. 50 ml.). The combined extracts were washed with brine, dried (MgSO.sub.4) and the solvent removed. The residual oil (12.6 g.) was chromatographed on silica gel and eluted with methanol-chloroform. Recovered 3,7,7-trimethyl-5,6,7,8-tetrahydroquinoline (7.6 g.) was obtained by elution with 2% methanol-chloroform. Elution with 5% methanol-chloroform gave 3,7,7-trimethyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (2 g., 15%) as a yellow solid which was recrystallised from benzene-hexane as pale yellow needles and converted to the hydrochloride by dissolving in ether and treating with an excess of dry HCl. The resultant solid was recrystallised from isopropyl alcohol to give the one and a quarter hydrate of the title compound as colourless needles m.p. 162.degree. C. (Found C, 53.3; H, 7.35; N, 9.5. C.sub.13 H.sub.18 N.sub.2 S.HCl.1.1/4H.sub.2 O requires: C, 53.3; H, 7.4; N, 9.55%).

Following the general procedure of Example 1 but using different molar ratios of trimethylsilylisothiocyanate (TMS.NCS): 8-Lithio-3-methyl-5,6,7,8-tetrahydroquinoline (LiTHQ) (prepared from equimolar amounts of butyl lithium and 3-methyl-5,6,7,8-tetrahydroquinoline) the following results were obtained.

3-Methyl-5,6,7,8-tetrahydroquinoline (7.3g, 0.05 mol.) was added to a solution of isopropylmagnesium bromide [prepared from isopropylbromide (6.15g, 0.05 mol), magnesium (1.44g, 0.06 mol) in ether (10 ml.)] and the solution heated at 60.degree. to remove the ether by distillation. Toluene (5 ml.) was added and the reaction mixture was heated at 120.degree. for 2 hours, cooled, diluted with toluene (30 ml) and this solution was added to a solution of trimethylsilyl isothiocyanate (7.85 g, 0.06 mol) in toluene (50 ml.) at 0.degree. C. The reaction mixture was stirred at room temperature for 12 hours, diluted with water (15 ml.) and the pH adjusted to 2.0 with Conc. HCl. The aqueous solution was extracted with ether and the extracts discarded. The aqueous solution was adjusted to pH 9.0 with Na.sub.2 CO.sub.3 and extracted with chloroform (3 .times. 50 ml.). The combined extracts were washed with brine, dried and the solvent removed to give a mixture of 3-methyl-5,6,7,8-tetrahydroquinoline and the title compound (5%) which was isolated by chromatography and identified by comparison with authentic material.

2-(2'-Oxocyclopentyl)methyl cyclohexanone was prepared from 2-(dimethylaminomethyl)cyclohexanone and cyclopentanone according to the method described in Ann. Chim. 1963, 53 (6), 819 and was isolated as a colourless oil in 80% yield b.p. 92.degree./0.05 mm.

2,3,5,6,7,8-hexahydro-1H-cyclopenta[b]-quinoline was prepared from 2-(2'-oxocyclopentyl)methyl cyclohexanone according to the method described in Ann. Chim., 1963, 53 (6), 819 and was isolated in 65% yield as a colourless oil b.p. 80.degree./0.05 mm.

The hydrochloride was prepared for characterisation by treating an ethereal solution of the base with ethereal HCl and was isolated as the hemihydrate as colourless needles from ethanol-ether. m.p. 104.degree. C.

(Found: C, 65.7, H, 7.8; N, 6.6. C.sub.12 H.sub.16 N.HCl. 1/2H.sub.2 O requires: C, 65.8; H, 7.8; N, 6.4%.)

A solution of 2,3,5,6,7,8-hexahydro-1H-cyclopenta[b]quinoline (5.19g., 0.03mol) in benzene (5 ml.) was cooled to 0.degree. C and treated with a 15% w/w solution of butyl lithium in hexane (13.5ml., 0.03 mol.) and allowed to stand at 0.degree. C for 1 hour.

The reaction mixture was treated dropwise with a solution of trimethylsilylisothiocyanate (4.5ml, 0.03mol) in benzene (2 ml.) with cooling in ice. The reaction mixture was stirred at 0.degree. C for an additional 1 hour, diluted with water (15 ml.) and the pH adjusted to 2.0 with 2N HCl. The solution was extracted with ethyl acetate (3 .times. 50 ml.) and the extracts discarded. The aqueous solution was adjusted to pH 9.0 with Na.sub.2 CO.sub.3 and extracted with chloroform (3 .times. 50 ml.).

The combined extracts were washed with brine, dried (MgSO.sub.4) and the solvent removed in vacuo.

The residual oil was diluted with n-hexane (100ml.) and cooled to 0.degree. C. The precipitated solid was filtered, recrystallised from isopropanol, dissolved in ether and treated with excess ethereal HCl. The resultant solid was recrystallised from ethanol-ether to give the hydrochloride monohydrate of the title compound (0.9g) m.p. 118.degree. C (Found: C, 54.6; H, 6.3; N, 10.0. C.sub.13 H.sub.16 N.sub.2 S.HCl.H.sub.2 O requires: C, 54.4; H, 6.6; N, 9.8%).

A solution of di-isopropylamine (11.11 g., 0.11 mol) in benzene (50 ml.) was cooled to 0.degree. C and treated portionwise with a 9% w/v solution of butyl lithium in hexane (79 ml., 0.11 mol.). After 45 minutes at 0.degree. C the solution was treated dropwise with 3-methyl-5,6,7,8-tetrahydroquinoline (14.7 g., 0.10 mol.) with rapid stirring and under an atmosphere of nitrogen. After 11/2 hours at 0.degree. C the red suspension was treated portionwise over 2 minutes with trimethylsilylisothiocyanate (14.7 ml., 0.11 mol.) and the reaction mixture stirred at 0.degree. C for 1/2 hour and at room temperature for 1 hour. The reaction mixture was diluted with water (25 ml.) and the pH adjusted to 2.0 with 2N HCl. The mixture was extracted with ethylacetate (3 .times. 25 ml.) and the combined extracts discarded. The aqueous solution was adjusted to pH 10.0 with sodium carbonate and extracted with chloroform (3 .times. 50 ml.). The combined extracts were washed with saturated brine (1 .times. 50 ml.), dried (MgSO.sub. 4) and the solvent removed in vacuo. The residual oily solid was triturated with n-hexane (100 ml.) and the solid filtered and recrystallised from isopropanol to give the title compound as pale yellow needles (8.9 g., 43%) m.p. 153.degree. C identical in all respects to authentic material. The filtrate was analysed by g.l.c. (10% SE30, T = 160.degree. C and identified as a mixture of 8-cyano-3-methyl-5,6,7,8-tetrahydroquinoline (1 g., 6% yield based on starting material) and recovered 3-methyl-5,6,7,8-tetrahydroquinoline (7.17 g., 48% yield based on starting material).

A solution of di-isopropylamine (33.3g, 0.33 mol) in benzene (150 ml) was cooled in ice and treated with 9% w/v butyl-lithium in hexane (237 ml, 0.33 mol). After 45 minutes the solution was treated with 5,6,7,8-tetrahydroquinoline (39.9 g, 0.3 mol) dropwise with stirring. After 1.5 hours trimethylsilyl-isothiocyanate (43.2 ml. 0.3 mol) was added and the resulting solution was allowed to stand at 0.degree. C for 0.5 hours and at room temperature for 1 hour. Water (50 ml.) was added and the resulting mixture acidified with 2N HCl. The acid solution was separated, washed with ethyl acetate and the pH was adjusted to 9 with solid sodium carbonate. Extraction with chloroform followed by drying of the extract over MgSO.sub.4, filtration and evaporation gave a thick gum which crystallised on trituration with n-hexane. Recrystallisation from methanol gave 5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (16g, 30%) m.p. 160.degree.. The hydrochloride was prepared by dissolution of the free base in hot iso-propyl alcohol adding ethereal HCl solution and allowing to crystallise. m.p. 263-4.degree..

(Found: C, 52.6; H, 6.0; N, 12.2. C.sub.10 H.sub.12 N.sub.2 S, HCl requires C, 52.5; H, 5.7; N, 12.3%).

A stirred solution of di-isopropylamine (12.2 ml, 0.085 mol) in benzene (100 ml) at 0.degree. C was treated dropwise with a 9% w/v solution of butyl lithium in hexane (62 ml, 0.085 mol). After 1 hour at 0.degree. C, 4-methyl-5,6,7,8-tetrahydroquinoline (12.8 g, 0.085 mol) was added dropwise and then after a further hour the anion was treated dropwise with trimethylsilyl isothiocyanate (12.2 ml, 0.095 mol). After 0.5 hours at 0.degree. C and 0.5 hours at room temperature the reaction mixture was diluted with water (50 ml) and the pH adjusted to 2.0 with conc. HCl. The aqueous layer was separated and adjusted to pH 10.0 with solid sodium carbonate and extracted with chloroform (3 .times. 50 ml) and the combined extracts were dried over MgSO.sub.4, filtered and the solvent was removed in vacuo. The residual oil was triturated with n-hexane to give 4-methyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (3.4g, 19%). The hydrochloride was prepared by dissolving in hot iso-propyl alcohol adding excess ethereal HCl solution and allowing to crystallise to give 4-methyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide, hydrochloride m.p. 212-3.degree. C. (Found: C, 54.9; H, 6.4; N, 11.5. C.sub.11 H.sub.14 N.sub.2 S. HCl requires C, 54.4; H, 6.2; N, 11.5%).

By the method described in Example 11 using di-isopropylamine (2 ml 0.014 mol) in benzene (20 ml), n-butyl lithium solution (9% w/v, 10 ml, 0.014 mol), 2-ethyl-5,6,7,8-tetrahydroquinoline (2.3 g, 0.014 mol) and trimethylsilyl isothiocyanate (2 ml 0.015 mol) was obtained 2-ethyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (600 mg, 20%) m.p. 73-5.degree. C (Found: C, 65.25; H, 7.6; N, 12.75. C.sub.12 H.sub.16 N.sub.2 S requires C, 65.4; H, 7.3; N, 12.7%).

By the method described in Example 11 using di-isopropylamine (4.45 ml 0.03 mol) in benzene (50 ml), n-butyl lithium solution (9% w/v, 13.5 g, 0.03 mol), 2-butyl-5,6,7,8-tetrahydroquinoline (6 g, 0.03 mol) and trimethylsilyl isothiocyanate (4.45 ml 0.033 mol) was obtained 2-butyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (1.2g, 15%) m.p. 54-6.degree. C. (Found: C, 68.0; H, 8.4; N, 11.2. C.sub.14 H.sub.20 N.sub.2 S requires C, 67.8; H, 8.1; N, 11.3%).

By the method described in Example 11 using di-isopropylamine (11.2 ml 0.077 mol) in benzene (150 ml), n-butyl lithium solution (9% w/v, 57 ml, 0.077 mol), 2-methyl-4-phenyl-5,6,7,8-tetrahydroquinoline (17.7 g, 0.077 mol), and trimethylsilyl isothiocyanate (11.2 ml 0.087 mol) was obtained 2-methyl-4-phenyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (1.2 g, 5%) m.p. 174-5.degree. C. (Found: C, 72.0; H, 6.7; N, 9.4. C.sub.17 H.sub.18 N.sub.2 S requires C, 72.3; H, 6.4; N, 9.9%).

By the method described in Example 11 using di-isopropylamine (4.9 ml 0.034 mol) in benzene (50 ml), n-butyl lithium solution (9% w/v, 25 ml, 0.034 mol), 3,4-dimethyl-5,6,7,8-tetrahydroquinoline (5.65g, 0.034 mol) and trimethylsilyl isothiocyanate (4.9 ml, 0.038 mol) was obtained 3,4-dimethyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (0.4 g, 5%) m.p. 163-5.degree. C.

(Found: C, 65.1; H, 7.8; N, 12.2. C.sub.12 H.sub.16 N.sub.2 S requires C, 65.4; H, 7.3; N, 12.2%).

To a solution of di-isopropylamine (4.44 g, 0.044 mole) in benzene (30 ml) at 0.degree. C under nitrogen was added with stirring a solution of n-butyl-lithium in hexane (9% w/v, 31.6 ml, 0.044 mole) and the resulting solution was stirred for 1 h at 0.degree. C. 3-Methyl-5,6,7,8-tetrahydroquinoline (5.86 g, 0.04 mole) was added and stirred for a further 11/2 h. The reaction mixture was treated with dimethylsilyl di-isothiocyanate (7.66 g, 0.044 mole) and the mixture was stirred for 0.5 h at 0.degree. C and at room temperature for 1 h. Water (50 ml) was added and the pH was adjusted to 2 with conc. HCl. The aqueous layer was separated, washed with ethyl acetate, and the pH was adjusted to 10 with solid sodium carbonate. The resulting mixture was extracted with ethyl acetate (3 .times. 50 ml). The combined extracts were dried (MgSO.sub.4), filtered, and the solvent was removed in vacuo. The residue was triturated with n-hexane to give 3-methyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (0.9 g, 15%).

A solution of 3-methyl-5,6,7,8-tetrahydroquinoline (1.43 g, 0.01 mole) in benzene (20 ml) was treated with n-butyl-lithium (15% w/w, 4.5 ml, 0.01 mole) and the solution was allowed to stand at room temperature for 0.5 h. The solution was then treated with a suspension of silicon tetraisothiocyanate (1.3 g, 0.005 mole) in benzene (5 ml) at 0.degree. C. After 10 min., water (50 ml) was added and the mixture was stirred for 0.5 h at room temperature and then acidified with conc. HCl. The aqueous layer was separated, washed with ethyl acetate and the pH was adjusted to 10 with solid Na.sub.2 CO.sub.3. The basic mixture was extracted with ethyl acetate (3 .times. 50 ml) and the combined organic extracts were dried (MgSO.sub.4), filtered and evaporated. The residue was triturated with n-hexane to give 3-methyl-5,6,7,8-tetrahydroquinoline-8-thiocarboxamide (0.2 g, 10%).