US 3,952,104 AGrant
Benzopyran-2-Carboxylic Acids in the Prevention of Asthmatic Symptoms
Issue Date:1976-04-20
•38 Claims
Abstract
There are described certain mono-chromone-2-carboxylic acids of formula, ##SPC1## Having one substituent in the 5 or 6 position or having 2 or more substituents in the 5,6,7 or 8 positions. There are also described processes for making the compounds and pharmacetuical compositions, for the treatment of asthma, containing the compounds.
Metadata
Assignee
- Fisons Limited
Inventors
- Hugh Cairns
- Albert Chambers
- Thomas Brian Lee
Application Information
Application Number:US 3826422
Filing Date:1973-07-26
Priority Date:1970-08-25
Art Unit:125
Classifications
IPC:
A61K 3135
Field of Search:
424283
Patent Drawings
This patent does not have any drawings.
Description
EXAMPLE 1
8-Allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. 3-Allyl-2-hydroxy-6-(2-hydroxypropoxy)acetophenone
A mixture of 58 parts of 3-allyl-2,6-dihydroxyacetophenone, 22.5 parts of propylene oxide and 0.25 parts of a 40% aqueous solution of benzytrimethylammonium hydroxide in 70 parts of dioxan was heated in a sealed vessel at 100.degree. C for 20 hours. The mixture was cooled and the vessel was opened. The dioxan was evaporated off and the residual oil was distilled to give 33 parts of 3-allyl-2-hydroxy-6-(2-hydroxypropoxy)acetophenone, boiling point, 158-168.degree. C/0.5-1.0 mm Hg., melting point 49.degree.-51.degree. C.
Analysis: Found: C, 67.1; H, 7.20%. C.sub.14 H.sub.18 O.sub.4 requires: C, 67.2; H, 7.20%.
b. 8-Allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid hemihydrate
A solution of 50 parts of 3-allyl-2-hydroxy-6-(2-hydroxypropoxy)-acetophenone in 75 parts of diethyl oxalate was added to a stirred solution of sodium ethoxide prepared from the dissolution of 18.5 parts of sodium in 320 parts of ethanol. The resulting mixture was stirred and heated on a stream-bath for 3.5 hours, then most of the ethanol was evaporated off and the residue was diluted with 1000 parts of water and was washed with ether. The aqueous solution was acidified with concentrated hydrochloric acid then it was extracted with 3 lots of 250 parts of chloroform. Evaporation of the chloroform extract left a residue, which was heated under reflux for 30 minutes with 320 parts of ethanol and 5 parts of concentrated hydrochloric acid. The mixture was evaporated and the residue was agitated with 60 parts of ether to give a solid precipitate, which was filtered off, dissolved in bicarbonate solution and reprecipitated upon acidification. The crude product was dried, then dissolved in a 9/1 ether/petrol (b.p. 40.degree.-60.degree. C) mixture from which, by careful evaporation, there was obtained 7.4 parts of 8-allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid hemihydrate, melting point 75.degree.-80.degree. C.
Analysis: Found: C, 60.9; H, 5.46%. C.sub.16 H.sub.16 O.sub.6.1/2H.sub./1/2H.sub.2 O requires: C, 61.3; H, 5.43%. Mass spectroscopy - Molecular Weight Found: 304. C.sub.16 H.sub.16 O.sub.6 requires: 304.
c. 8-Allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, sodium salt
A solution of 1.7 parts of 8-allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid hemihydrate and 0.42 parts of sodium bicarbonate in 25 parts of water was prepared, filtered and freeze-dried to give 1.8 parts of 8-allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, sodium salt.
EXAMPLE 2
6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. 6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
A mixture of 5 parts of 2,4-di-t-butylphenol, 3.55 parts of acetylene dicarboxylic acid dimethyl ester and four drops of benzyl-trimethylammonium hyroxide were heated on a steam bath for 20 min. The 2,4-di-t-butylphenoxyfumaric acid dimethyl ester formed was hydrolysed by heating with 2.8 parts of sodium hydroxide in 15 parts by volume of water and 8 parts by volume of methanol. The hydrolysis was complete when one layer was obtained. The methanol was boiled off and the solution was cooled and acidified with concentrated hydrochloric acid. The crude 2,4-di-t-butylphenoxyfumaric acid was liberated as an oil, and was extracted into ether. This acid was purified by extraction with sodium bicarbonate solution which was washed well with ether. Acidification of the sodium bicarbonate solution gave the purified fumaric acid which was extracted again into ether. The dried ether extracts were evaporated to give the fumaric acid as an oil.
The di-t-butylphenoxyfumaric acid was cyclised by the cautious addition of 11 parts by volume of chlorosulphonic acid at 5.degree. C. The mixture was kept for 20 min. at room temperature and then poured into water. The product which solidified, was washed well with petroleum ether (b.p. 40-60) then crystallised from aqueous ethanol to give 2.8 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, melting point 230.degree.-232.degree. C.
Analysis Found: C, 71.4; H, 7.42%. C.sub.18 H.sub.22 O.sub.4 requires: C, 71.5; H, 7.33%.
b. 6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, sodium salt
To 5.5. parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid was added 1.4 parts of sodium bicarbonate in 150 parts by volume of water. The mixture was gently heated on a steam bath until nearly all the acid had dissolved. The neutral solution was filtered and freeze-dried to give 5 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, sodium salt.
EXAMPLE 3
a. 6,8-Di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, piperidine salt
A solution of 5.0 parts of 6,8-di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid in 1.7 parts of piperidine and 50 parts of water was filtered and freeze-dried. The resultant pale yellow powder was washed with hot petroleum ether (b.p. 60.degree.-80.degree. C), giving 5.6 parts of 6,8-di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, piperidine salt as a colourless powder, melting point 191.degree.-194.degree. C, (decomposition).
Analysis Found: C, 71.5; H, 8.65; N, 3.57%. C.sub.23 H.sub.33 NO.sub.4 requires: C, 71.29; H, 8.58; H, 3.61%.
Spectral confirmation
NMR showed the 3-proton of the benzopyran ring as a singlet at 3.23.tau. , and broad signals at 7.05.tau. and 8.60.tau. were assigned to the piperidine ring protons (solvent: deuterium oxide).
b. 6,8-Di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethylamine salt
5.0 parts of 6,8-di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid were added to a mixture of 1.5 parts of 70% aqueous ethylamine and 50 parts of water. The resultant pale yellow solution was filtered and freeze-dried, and the resulting solid was washed with ether to afford 5.3 parts of 6,8-di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethylamine salt as a colourless powder, melting point 205.degree.-206.degree. C (decomposition).
Analysis Found: C, 69.2; H, 8.72; N, 3.74%. C.sub.20 h.sub.29 NO.sub.4 requires: C, 69.13; H, 8.41; N, 4.03 %.
Spectral confirmation
The 3-proton of the benzopyran ring gave an NMR singlet at 3.20.tau. , and the N-ethyl group gave a quartet at 7.00.tau. and a triplet at ca. 8.80.tau. (Solvent: deuterium oxide).
EXAMPLE 4
6-Allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. 2-Allyloxy-6-hydroxyacetophenone
A mixtue of 15.2 parts of 2,6-dihydroxyacetophenone, 12.1 parts of allyl bromide and 13.8 parts of potassium carbonate in 100 parts of acetone was stirred and refluxed for 7 hours. The acetone was evaporated and water, dilute hydrochloric acid and ether were added.
The ethereal layer was separated, dried and evaporated to leave a yellow oil. This oil was distilled and the fraction boiling at 128.degree.-132.degree. C at 0.15 mms of mercury was collected. This oil solidified on cooling but thin layer chromatography showed it to be a mixture of produce and starting material. The mixture was chromatographed on alumina using ether as eluent. Evaporation of the ether gave a yellow solid which crystallised from petroleum ether (b.p. 40.degree.-60.degree. C) to give 10.0 parts of 2-allyloxy-6-hydroxyacetophenone as yellow needles, melting point 45.5.degree.-46.5.degree. C.
Analysis Found: C, 68.4; H, 5.95%. C.sub.11 H.sub.12 O.sub.3 requires: C, 68.73; H, 6.29%.
b. 5-Allyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester
To a stirred solution of 1.2 parts of sodium in 35 parts of ethanol was added a solution of 3.85 parts of 2-allyloxy-6-hydroxyacetophenone and 10 parts of diethyl oxalate in 50 parts of diethyl ether. The mixture was stirred and refluxed for 5 hours, then poured into 500 parts of diethyl ether.
The product was then extracted into water (3 .times. 120 ml) and the aqueous solution was acidified with concentrated hydrochloric acid and extracted in chloroform (3 .times. 80 ml). The chloroform solution was dried over sodium sulphate and evaporated to leave a red oil. This oil was dissolved in 30 parts of ethanol, a few drops of concentrated hydrochloric acid were added and the solution was refluxed for 5 minutes. On cooling, 2.7 parts of a solid crystallised out, melting point 95.degree.-97.degree. C which was shown to be a mixture of acid and ester by thin layer chromatography. This solid was washed with aqueous sodium bicarbonate solution and filtered to leave 2.5 parts of 5-allyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester, melting point 122.degree.-123.degree. C which was one spot on a thin layer chromatogram.
Analysis Found: C, 65.5; H, 5.10%. C.sub.15 H.sub.14 O.sub.5 requires: C, 65.7; H, 5.15%.
c. 6-Allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester
3.44 Parts of 5-allyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester was heated at 200.degree. C for 11/2 hours. On cooling, the mass solidified and was then dissolved in ether and chromotographed on alumina using ether as eluent. The ethereal solution was concentrated whence 2.5 parts of 6-allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester crystallised as yellow plates, melting point 99.degree.-100.degree. C.
Analysis Found: C, 65.5; H, 5.25%. C.sub.15 H.sub.14 O.sub.5 requires: C, 65.69; H, 5.15%.
d. 6-Allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid
A suspension of 2.0 parts of 6-allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester in aqueous sodium bicarbonate solution was stirred and heated at 100.degree. C till solution was complete. The solution was treated with charcoal, filtered and acidified with dilute hydrochloric acid to give a yellow precipitate. This solid was crystallised from ethanol to give 0.84 parts of 6-allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid as yellow needles, melting point 222.degree.-224.degree. C.
Analysis Found: C, 63.7; H, 4.16%. C.sub.13 H.sub.10 O.sub.5 requires: C, 63.4; H, 4.09%.
e. 6-Allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt
A solution of 0.7 parts of 6-allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid and 0.24 parts of sodium bicarbonate in 50 parts of water was freeze-dried to give 0.7 parts of 6-allyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt as a yellow solid.
EXAMPLE 5
6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. 3,5-Di-t-butyl-2-hydroxyacetophenone
A mixture of 20.6 parts of 2,4-di-t-butylphenol, 19.2 parts of acetic anhydride and 80 parts of boron trifluoride: acetic acid complex was heated for 2 hours at 100.degree. C. After pouring into ice cold dilute hydrochloric acid the mixture was extracted with ether which was washed with water, sodium hydrogen carbonate solution and water. Addition of 40% sodium hydroxide solution caused the precipitation of sodium 2,4-di-t-butylphenate. The ether was decanted, washed with water, dried over magnesium sulphate and evaporated to a brown oil. Distillation under reduced pressure gave a pale yellow liquid: b.sub.0.45 100.degree. C, which crystallised to give a compound, m.p. 45.degree.-46.degree. C.
Analysis Found C, 77.7; H, 10.0%. C.sub.16 H.sub.24 O.sub.2 requires: C, 77.37; H, 9.74%.
b. 6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester
A solution of sodium ethoxide, prepared from 0.46 parts of sodium and 25 parts of ethanol, was added with stirring to a mixture of 1.24 parts of 3,5-di-t-butyl-2-hydroxyacetophenone and 1.35 parts of diethyl oxalate dissolved in 50 parts of diethyl ether. The mixture was stirred at reflux for two hours, poured into dilute hydrochloric acid and extracted with chloroform. The chloroform was washed with water and evaporated to a red oil. The oil was boiled for 10 minutes with ethanol containing 1.0 parts of concentrated hydrochloric acid. On cooling, the solution deposited crystals which were collected to yield 1.3 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester, m.p. 130.degree.-131.5.degree. C.
Analysis Found: C, 73.0; H, 8.01%. C.sub.20 H.sub.26 O.sub.4 requires: C, 72.7; H, 7.93%.
c. 6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt
To a chilled solution of 1.02 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester in 100 parts of ethanol was added 3.1 parts of N sodium hydroxide solution. After the solution had been stirred at room temperature for one hour the solvent was evaporated and the remaining solid was triturated with ether and filtered off to yield 0.93 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt.
EXAMPLE 6
5-(3-Methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. 8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester
A solution of 53 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid in 750 parts of ethanol containing 2.0 parts of concentrated sulphuric acid was heated under reflux for 20 hours. The ethanol was removed by distillation and a solution of the residual oil in chloroform was washed with aqueous sodium bicarbonate solution and water. After drying, the chloroform solution was evaporated to leave an oil. This oil was dissolved in diethyl ether. On standing 45 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester crystallised as yellow needles, melting point 104.degree.-106.degree. C.
Analysis Found: C, 69.70; H, 7.22%. C.sub.20 H.sub.24 O.sub.5 requires: C, 69.75; H, 7.02%.
Spectral confirmation
Molecular weight = 344 by mass spectrometry.
NMR (CDCl.sub.3 solution) showed the ethyl ester protons as a quartet and triplet at 5.63.tau. and 8.62.tau. respectively.
b. 5-(3-Methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
A suspension of 12.05 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester in 225 parts of ethanol was treated with 0.2 parts of 5% palladium/charcoal catalyst and hydrogenated at a pressure of 4 atmospheres. After 30 minutes the hydrogen uptake ceased and the reaction mixture was filtered. The volume of the filtrate was reduced and then water was added. On standing 10.2 parts of 5-(3-methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester crystallised as yellow needles, melting point 64.5.degree.-65.degree. C. This material was stirred with a hot aqueous solution of sodium bicarbonate containing some ethanol for two hours. The resulting solution was cooled, washed with diethyl ether and acidified with dilute hydrochloric acid. The yellow precipitate was collected and crystallised from aqueous ethanol to give 7.2 parts of 5-(3-methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid as yellow needles, melting point 166.degree.-167.degree. C.
Analysis Found: C, 67.50; H, 6.97%. C.sub.18 H.sub.22 O.sub.5 requires: C, 67.90; H, 6.97%.
SPECTRAL CONFIRMATION
Molecular weight = 318 by mass spectrometry.
Infra-red showed the acid carbonyl absorption at 1740 cm.sup.-.sup.1 and the benzopyran ring carbonyl absorption at 1640 cm.sup.-.sup.1.
NMR showed the 3-proton of the benzopyran ring as a singlet at 3.4.tau..
c. 5-(3-Methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, sodium salt
A mixture of 4.77 parts of 5-(3-methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid and 1.26 parts of sodium bicarbonate was dissolved in 100 parts of water. The solution was filtered and freeze-dried. The hygroscopic solid was crystallised from ethanol to give 3.3 parts of 5-(3-methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, sodium salt as a white solid.
Spectral confirmation
Infra-red showed the carboxylate ion as a broad absorption about 1620 cm.sup.-.sup.1.
NMR showed the 3-proton of the benzopyran ring as a singlet at 3.48.tau..
EXAMPLE 7
5-(3-Methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. 2-Hydroxy-4-(3-methyl-n-butoxy)propiophenone
A mixture of 116 parts of 2,4-dihydroxypropiophenone, 151 parts of isoamyl bromide and 140 parts of potassium carbonate in 300 parts of acetone were stirred and heated under reflux for 3 days. The reaction mixture was cooled, filtered and the filtrate was evaporated to dryness in vacuo. The resulting oil was dissolved in 500 parts of ether and 200 parts of 2N hydrochloric acid. The ether solution was washed with 100 parts of 2N hydrochloric acid, 6 lots of 50 parts of 5% aqueous sodium hydroxide solution, water, 2N hydrochloric acid, water, dried over anhydrous magnesium sulphate, filtered and evaporated to dryness in vacuo. The resulting colourless oil rapidly crystallised to give 108 parts of 2-hydroxy-4-(2-methyl-n-butoxy)propiophenone as a white solid, melting point 43.5.degree.-44.0.degree. C.
Analysis Found: C, 70.79; H, 8.42%. C.sub.14 H.sub.20 O.sub.3 requires: C, 71.16; H, 8.53%.
Spectral confirmation
Molecular weight = 236 by mass spectrometry.
N.m.r. showed only one hydroxyl proton at -2.55.tau. in carbon tetrachloride, characteristic of a hydrogen bonded phenol.
b. 5-(3-Methyl-n-butoxy)-2-propylphenol
100 parts of zinc wool, 10 parts of mercuric chloride, 5 parts of concentrated hydrochloric acid and 150 parts of water were shaken together for 5 minutes. The aqueous layer was decanted and the amalgamated zinc was covered with 150 parts of water and 200 parts of concentrated hydrochloric acid. A solution of 47.2 parts of 2-hydroxy-4-(3-methyl-n-butoxy)propiophenone in 200 parts of dioxan were added and the mixture was heated under reflux overnight. The reaction mixture was filtered, cooled and extracted with 4 lots of 100 parts of ether. The latter were combined and washed with 5 lots of 500 parts of water, dried over magnesium sulphate, filtered and evaporated to dryness in vacuo to a colourless oil, which solidified to give 43.7 parts of 5-(3-methyl-n-butoxy)-2-propyphenol as a colourless crystalline solid, melting point 40.0.degree.-40.5.degree..
Analysis Found: C, 75.94; H, 9.78%. C.sub.14 H.sub.22 O.sub.2 requires: C, 75.63; H, 9.97%.
Spectral confirmation
Molecular weight = 222 by mass spectrometry.
N.m.r. displayed the presence of the propyl group and the phenolic hydroxy proton now appeared at 4.05.tau..
The infra-red spectrum showed an intense broad O-H str peak at 3420 cm.sup.-.sup.1 and two resolved peaks at 1620 and 1595 cm.sup.-.sup.1 for the aromatic C=C stretch.
c. 5-(3-Methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
i. 22.2 Parts of 5-(3-methyl-n-butoxy)-2-propylphenol were dissolved in 14.2 parts of dimethyl acetylene dicarboxylate followed by addition of 0.25 parts of benzyl trimethylammonium hydroxide. The mixture was heated at 100.degree. C for 45 minutes, after which 45 parts of 25% aqueous sodium hydroxide solution were added and the heating at 100.degree. C was continued for a further 21/2 hours. The resulting homogeneous solution was cooled and acidified to pH 1 with 20% v/v sulphuric acid. The yellow precipitate, produced, was extracted with 4 lots of 200 parts of ether and the latter was washed with 2N sulphuric acid, water, dried over magnesium sulphate, filtered and evaporated to dryness in vacuo. The resulting yellow oil quickly solidified to give 30.9 parts of a yellow solid. Recrystallisation from ethyl acetate/petrol (b.p. 60.degree.-80.degree. C) afforded 23.9 parts of 1-[2-propyl-5-(3-methyl-n-butoxy)phenoxy]ethylene-1,2-dicarboxylic acid.
ii. 10 Parts of 1-[2-propyl-5-(3-methyl-n-butoxy)phenoxy]ethylene-1,2-dicarboxylic acid were dissolved in 92 parts of concentrated sulphuric acid and allowed to stand at room temperature for 30 minutes. The mixture was filtered through glass wool into stirred ice water and the resulting yellow-brown syrup was extracted with ethyl acetate. The latter was washed with 2N sulphuric acid, water, dried over magnesium sulphate, filtered and evaporated to dryness in vacuo to yield a brown oil, which crystallised from ethyl acetate/petrol (b.p. 60.degree.-80.degree. C). The yellow crystals were collected and dried in vacuo, giving 1.24 parts of 5-(3-methyl-n-butoxy)-8-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, melting point 166.degree.-167.degree. C.
Spectral confirmation
This product was shown to be identical to that prepared in Example 6.
EXAMPLE 8
6,8-Di-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. 6,8-Di-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
A solution of 6.2 parts of 6,8-diallyl-4-oxo-4H-1-benzopyran-2-carboxylic acid in 100 parts of ethanol was hydrogenated at room temperature for 45 minutes at a pressure of 45 p.s.i. at the presence of 5% palladised charcoal. The solution was filtered and evaporated to afford 6.1 parts of 6,8-di-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, melting point 183.degree.-184.degree. C after recrystallisation from a mixture of ethyl acetate and petroleum ether (b.p. 60.degree.-80.degree. C).
Analysis Found: C, 70.0; H, 6.75%. C.sub.16 H.sub.18 O.sub.4 requires: C, 70.05; H, 6.61%.
Spectral Confirmation
Molecular weight =274 by mass spectrometry.
IR acid carbonyl absorption occurred at 1725 cm.sup..sup.-1 and benzopyran carbonyl absorption at 1620 cm.sup..sup.-1.
The N.M.R. spectrum contained a singlet at 2.7.tau. due to the benzopyran ring 3-proton (solvent: deuterochloroform).
b. 6,8-Di-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, sodium salt
A mixture of 4.7 parts of 6,8-di-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid and 1.37 parts of sodium bicarbonate was dissolved in 50 parts of water. The filtered solution was freeze-dried to give 4.5 parts of 6,8-di-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, sodium salt.
Spectral Confirmation
IR carbonyl absorption occurred at 1620 cm.sup..sup.-1.
The N.M.R. spectrum showed a singlet at 3.3.tau. due to the benzopyran ring 3-proton (solvent: deuterium oxide).
EXAMPLE 9
5-(3-Methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. Ethyl 5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylate, thallium salt
A freshly prepared solution of 164.8 parts of thallous ethoxide in 500 parts of chloroform was quickly added to a stirred solution of 160 parts of 5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester in 2000 parts of chloroform. A further 1000 parts of chloroform was added and the mixture was stirred for twenty minutes. The precipitated product was collected by filtration, washed with chloroform and dried to give 303 parts of ethyl 5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylate, thallium salt m.p.>300.degree.C.
b. 5-(3-Methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid
A stirred mixture of 21.87 parts of ethyl 5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylate, thallium salt and 99 parts of 1-iodo-3-methylbutane was refluxed for one hour. After cooling, chloroform was added to the reaction mixture and the insoluble thallium salts were removed by filtration. The chloroform filtrate was washed with cold N sodium hydroxide solution, then washed with water and the volatile components were removed by evaporation. Sodium bicarbonate solution was added to the oily residue obtained and the mixture was stirred at 100.degree.C for two hours. After cooling, the solution was extracted with chloroform and the aqueous layer was separated. This aqueous extract was acidified with 2N hydrochloric acid and the solid which precipitated was collected by filtration, washed with water and dried to give 6.16 parts of 5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, m.p. 181.degree.-183.degree.C (after crystallisation from ethyl acetate/light petroleum).
Spectral Confirmation
Molecular weight = 276 by mass spectroscopy
C.sub.15 H.sub.16 O.sub.5 requires 276
EXAMPLE 10
5-(3-Methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid
A mixture of 23.4 parts of 5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester, 19.8 parts of 1-iodo-3-methybutane, 11.65 parts of anhydrous potassium carbonate and 500 parts of acetone was refluxed for thirteen days. After cooling, the reaction mixture was filtered and the volatile components of the filtrate were removed by evaporation to give a residue which was dissolved in chloroform. The chloroform extract was washed with cold N sodium hydroxide solution, then with water and evaporated. The oily residue obtained was stirred in sodium bicarbonate solution at 100.degree.C for two hours. After cooling, the solution was extracted with chloroform and the aqueous layer was separated. The aqueous extract was acidified with 2N hydrochloric acid and the mixture was extracted with chloroform. Evaporation of the chloroform gave 8.79 parts of 5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid m.p. 181.degree.-183.degree.C (after crystallisation from ethyl acetate/light petroleum).
Spectral Confirmation
Molecular weight = 276 by mass spectroscopy.
C.sub.15 H.sub.16 O.sub.5 requires 276
EXAMPLE 11
5-(3-Methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid
A mixture of 23.4 parts of 5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester, 39.6 parts of 1-iodo-3-methylbutane, 11.65 parts of anhydrous potassium carbonate and 500 parts of isobutylmethyl ketone was refluxed for six days. After cooling, the reaction mixture was filtered and the volatile components of the filtrate were removed by evaporation to give a residue which was dissolved in chloroform. The chloroform extract was washed with cold N sodium hydroxide solution, then with water and evaporated.
The oily residue obtained was stirred in sodium bicarbonate solution at 100.degree.C for four hours. After cooling, the solution was extracted with chloroform and the aqueous layer was separated. The aqueous extract was acidified with 2N hydrochloric acid and the mixture was extracted with chloroform. Evaporation of the chloroform gave 7.05 parts of 5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid m.p. 179.degree.-180.degree.C (after crystallisation from ethyl acetate/light petroleum).
EXAMPLE 12
6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
a) 6,8-Di-t-butyl-2,3-dihydro-4-oxo-4-H-1-benzopyran-2-carboxylic acid, ethyl ester
A solution of 12.0 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester in 150 parts of ethanol was hydrogenated, using Raney Nickel as catalyst, until one equivalent of hydrogen has been absorbed. The catalyst was removed by filtration and the ethanol was evaporated to give a residue which was chromatographed on silica gel. Careful elution with a 1:1 mixture of chloroform and light petroleum (b.p. 60.degree.-80.degree.C) gave 3.73 parts of 6,8-di-t-butyl-2,3-butyl-2,3-dihydro-4-oxo-4H-benzopyran-2-carboxylic acid, ethyl ester, m.p. 61.degree.-63.degree.C, as a yellow solid.
Spectral Confirmation
Molecular weight = 332 by mass spectroscopy
C.sub.20 H.sub.28 O.sub.4 requires 332
b. 6,8-Di-t-butyl-2,3-dihydro-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester
i. 2,4-Di-t-butylphenoxyfumaric acid
A mixture of 2060 parts of 2,4-di-t-butylphenol, 1040 parts by volume of acetylene dicarboxylic acid dimethyl ester and 10 parts of benzyltrimethylammonium hydroxide was heated on a steam bath until a rapid exothermic reaction had occurred. The mixture was cooled to room temperature and ether was added. The ether extract was washed with N sodium hydroxide solution, sodium chloride solution and water, then the ether was removed by evaporation. The residue obtained was refluxed for three hours with 5000 parts of 5N sodium hydroxide solution. After cooling the mixture to room temperature, the solid which precipitated was removed by filtration.
The filtrate was evaporated under reduced pressure to give a residue which was extracted into ether. The stirred ethereal extract was acidified with concentrated hydrochloric acid, washed with sodium chloride solution and dried. 5000 parts of benzene were added and solvent was removed from the mixture by distillation until a head temperature of 67.degree.C was obtained. The resulting solution on cooling to room temperature gave 1429 parts of 2,4-di-t-butylphenoxyfumaric acid as a yellow solid, m.p. 188.degree.C (after recrystallisation from benzene).
Analysis Found: C, 67.48; H, 7.53%. C.sub.18 H.sub.24 O.sub.5 requires: C, 67.50; H, 7.50%.
Spectral Confirmation
Molecular weight = 320 by mass spectroscopy.
ii. 2,4-Di-t-butylphenoxysuccinic acid
3400 parts of a 21/2% sodium amalgam were added over one hour to a stirred solution of 320 parts of 2,4-di-t-butylphenoxyfumaric acid in 2200 parts of N sodium hydroxide solution. The reaction mixture was stirred for two hours further, then water was added to dissolve the white solid which had formed. The solution was decanted from the mercury, extracted with chloroform and the aqueous layer was separated.
Concentrated hydrochloric acid was added to the stirred aqueous extract until the solution was strongly acidic. The precipitated white solid was collected by filtration washed with water and dried to give 231 parts of 2,4-di-t-butylphenoxysuccinic acid, m.p. 177.degree.-181.degree.C.
Spectral Confirmation
Molecular weight = 322 by mass spectroscopy
C.sub.18 H.sub.26 O.sub.5 requires 322
iii. 6,8-Di-t-butyl-2,3-dihydro-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester
A mixture of 50 parts of 2,4-di-t-butylphenoxysuccinic acid and 100 parts of concentrated sulphuric acid was stirred at room temperature for three hours. The resulting solution was poured into water, excess sodium bicarbonate solution was added and the basic solution was extracted with ether. The aqueous extract was acidified with concentrated hydrochloric acid and the product was extracted into ether. The ether extract yielded a residue which was esterified by refluxing for three hours with 200 parts of a 3% v/v solution of ethanol containing sulphuric acid. After cooling, water was added and the esterified product was extracted into ether. The ether extract was washed with sodium bicarbonate solution and water, dried and the ether was removed by evaporation. Chromatography of the resulting residue on silica gel, eluting with a 3:2 mixture of chloroform and light petroleum (b.p. 40.degree.-60.degree.C), gave 1 part of 6,8-di-t-butyl-2,3-dihydro-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester as a semi-solid.
Spectral Confirmation
Molecular weight = 332 by mass spectroscopy.
C.sub.20 H.sub.28 O.sub.4 requires 332
c. 6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester
A mixture of 3.32 parts of 6,8-di-t-butyl-2,3-dihydro-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester and 1.84 parts of N-bromosuccinimide was refluxed in 150 parts of carbon tetrachloride for six hours. The solution was cooled and then washed with water. The carbon tetrachloride extract was dried and the solvent was removed by evaporation. The resulting brown residue was crystallised from light petroleum (b.p. 40.degree.-60.degree.C) to give 1.69 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester, m.p. 130.degree.-131.5.degree.C. The ester was hydrolysed to the sodium salt using the process of Example 5.
EXAMPLE 13
6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester
A mixture of 3.32 parts of 6,8-di-t-butyl-2,3-dihydro-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester and 3.33 parts of selenium dioxide was refluxed in 35 parts of xlene for eight hours. The reaction mixture was cooled, ether was added and the insoluble inorganic products were removed by filtration. Evaporation of the volatile components of the filtrate gave a brown residue which crystallised from light petroleum (b.p. 40.degree.-60.degree.C) to give 1.54 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester, m.p. 130.degree.-131.5.degree.C.
EXAMPLE 14
6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester
A mixture of 3.32 parts of 6,8-di-t-butyl-2,3-dihydro-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester and 9.9 parts of lead tetraacetate was refluxed in 30 parts of glacial acetic acid for 6.5 hours. A further 9.9 parts of lead tetraacetate were added and the reflux was continued for another 3.25 hours. After cooling, the reaction mixture was extracted with ether. The ether extract was washed with sodium bicarbonate solution and water and was dried. Evaporation of the ether gave an oil which was chromatographed on silica gel using a 9:1 mixture of toluene and ethanol for elution. This yielded 0.23 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester, m.p. 130.degree.-131.5.degree.C (after crystallisation from light petroleum (b.p. 40.degree.-60.degree.C). This product was shown by spectral means to be the same as that of Example 13.
EXAMPLE 15
6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester
A mixture of 3.32 parts of 6,8-di-t-butyl-2,3-dihydro-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester, 1.56 parts of triphenylmethyl perchlorate and 30 parts of acetic anhydride was heated at 100.degree.C for thirty minutes. After cooling, the reaction mixture was poured into sodium bicarbonate solution and the resulting mixture was washed with water, dried and the ether was evaporated to give an oil which was chromatographed on silica gel using a 1:1 mixture of chloroform and light petroleum (b.p. 40.degree.-60.degree.C) for elution. This yielded 0.14 parts of 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, ethyl ester, m.p. 130.degree.-131.5.degree.C (after crystallisation from light petroleum (b.p. 40.degree.-60.degree.C). This product was shown by spectral means to be the same as that prepared in Example 13.
EXAMPLE 16
5-(3-Methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid
A mixture of 1.0 parts of 5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carbonitrile, 20 parts of dioxan and 20 parts of dilute hydrochloric acid was heated at reflux for 24 hours. The dioxan was evaporated, and the reaction mixture was extracted with chloroform. The chloroform layer was then extracted with saturated sodium bicarbonate solution which on acidification gave 0.30 parts of 5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, melting point 180.degree.-182.degree.C. The melting point, IR spectrum and thin layer chromatographic properties of the product were identical with those of the compound prepared in Example 9.
EXAMPLE 17
6,8-Dipropyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
A mixture of 1.0 parts of 6,8-dipropyl-4-oxo-4H-1-benzopyran-2-carboxamide, 20 parts of glacial acetic acid, and 20 parts of a solution of hydrogen bromide (45% weight/volume) in glacial acetic acid was heated at reflux for 3 hours, then added to water and extracted with chloroform. Extraction of the organic layer with saturated sodium bicarbonate solution, followed by acidification of the bicarbonate layer, afforded 0.16 parts of 6,8-dipropyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, melting point 183.degree.-184.degree.C (decomposition). The melting point, IR spectrum and thin layer chromatographic properties of this material were identical with those of the compound prepared in Example 8.
EXAMPLE 18
8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid
To a stirred solution of 5.0 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carbonyl chloride in 50 parts of dichloroethane was added 10 parts of water. The mixture was stirred at 50.degree. C for one hour then evaporated to dryness. The resulting solid was washed thoroughly with aqueous sodium bicarbonate solution. Acidification of the bicarbonate solution with concentrated hydrochloric acid gave a yellow precipitate which was crystallised from ethyl acetate to give 2.6 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, m.p. 198.degree.-199.degree. C.
EXAMPLE 19
5-(3-Methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid hydroxylamine salt
An alkaline solution of hydroxlamine was prepared by mixing 4 parts of sodium hydrogen carbonate with 3.6 parts of hydroxylamine hydrochloride in 100 parts of refluxing ethanol; after 15 minutes the precipitated sodium chloride was filtered off.
To a stirred suspension of 5 parts of 5-(3-methyl-n-butoxy-4-oxo-4H-1-benzopyran-2-carboxylic acid, in 50 parts of refluxing ethanol, was added 40 parts of the hydroxylamine filtrate. The mixture was stirred and refluxed for a further 15 minutes, allowed to cool, and the white solid was filtered off to yield 5 parts of 5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid hydroxylamine salt.
Analysis Found: C, 57.89; H, 6.18; N, 4.46%. C.sub.15 H.sub.19 NO.sub.6 requires: C, 58.24; H, 6.19; N, 4.53%.
EXAMPLE 20
8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid ethylamine salt
An aqueous solution containing 5 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid and 1.07 parts of ethylamine (70% v/v) was filtered and freeze-dried to give 5 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid ethylamine salt hemihydrate as a pale yellow solid, m.p. 135.degree.-137.degree. C.
Analysis Found: C, 64.35; H, 7.34; N, 3.48%. C.sub.20 H.sub.27 NO.sub.5.sup.. 1/2H.sub.2 O requires: C, 64.90; H, 7.57; N, 3.79%.
EXAMPLE 21
8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid piperidine salt
A solution of 5 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid and 1.35 parts of piperidine in 50 parts of ethanol was refluxed for 2 hours. The resulting solution was filtered and evaporated to an oil which was dissolved in dry benzene. The benzene solution was evaporated to give an oil which was triturated with petroleum ether (b.p. 40.degree.-60.degree. C) to give 6 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid piperidine salt as a white solid m.p. 106.degree.-107.degree. C.
Analysis Found: C, 68.81; H, 7.78; N, 3.49%. C.sub.23 H.sub.31 NO.sub.5 requires: C, 68.59; H, 7.77; N, 3.30%.
EXAMPLE 22
8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid calcium salt
To a solution of 4.93 g of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt in 50 parts of water was added a saturated solution of 1.2 parts of calcium nitrate in water. The resulting calcium salt was filtered off, washed with water and dried to give 5 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid calcium salt monohydrate as a white solid.
Analysis Found: C, 62.89; H, 5.91%. C.sub.36 H.sub.38 CaO.sub.10.sup.. H.sub.2 O requires: C, 62.80; H, 5.82%.
EXAMPLE 23
8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid tris-(hydroxymethyl)methylamine salt
A solution of 5.66 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid and 2.16 parts of tris-(hydroxymethyl)methylamine in 50 parts of ethanol was refluxed for 2 hours. The solution was filtered and evaporated to an oil which was triturated with ether to give 7.1 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid tris-(hydroxymethyl)methylamine salt hemihydrate as a pale yellow solid.
Analysis Found: C, 59.52; H, 7.43; N, 3.06%. C.sub.20 H.sub.31 NO.sub.8.sup.. 1/2H.sub.2 O requires: C, 59.20; H, 7.18; N, 3.14%.
EXAMPLE 24
6,8-Di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid (.beta.-diethylamino)ethyl ester hydrochloride
A mixture of 10 parts of 6,8-di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt and 8 parts of (.beta.-diethylamino)ethyl chloride was shaken for 1 hour in benzene and refluxed for a further 10 hours. After cooling the inorganic solid was filtered off and the filtrate was evaporated to leave an oil which was heated at 110.degree. C at 15 mm pressure for 3 hours to remove any remaining (.beta.-diethylamino)ethyl chloride. The oil was then dissolved in diethyl ether and to this solution was added ethereal hydrogen chloride. The resulting solid was filtered off and crystallised from dioxan/diethyl ether to give 7.01 parts of 6,8-di-tert-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid (.beta.-diethylamino)ethyl ester hydrochloride hemihydrate as a white solid.
Analysis Found: C, 64.91; H, 8.23; N, 3.09%. C.sub.24 H.sub.36 ClNO.sub.4.sup.. 1/2H.sub.2 O requires: C, 64.50; H, 8.06; N, 3.14%.
The structure was confirmed by nuclear magnetic resonance and mass spectroscopy.
EXAMPLE 25
8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1benzopyran-2-carboxylic acid(.beta.-diethylamino)ethyl ester hydrochloride
A mixture of 10.14 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt and 8.13 parts of (.beta.-diethylamino)ethyl chloride was refluxed in benzene for 10 hours. The inorganic material was then filtered off and the filtrate was evaporated under vacuum to leave an oil. The oil was heated for 3 hours at 110.degree. C at 15 mm pressure to remove the last traces of (.beta.-diethylamino)ethyl chloride. The residual oil was cooled and dissolved in diethyl ether. To this solution was added ethereal hydrogen chloride and the resulting solid was filtered off and crystallised from dioxan/diethyl ether to give 9.8 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid (.beta.-diethylamino)ethyl ester hydrochloride hemihydrate as a white solid m.p. 125.degree. over a range.
Analysis Found: C, 61.67; H, 7.50; N, 3.02%. C.sub.24 H.sub.34 ClNO.sub.5.sup.. 1/2H.sub.2 O requires: C, 61.95; H, 7.60; N, 3.04%.
The structure was confirmed by nuclear magnetic resonance and mass spectroscopy.
EXAMPLE 26
8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid pivaloyloxymethyl ester
To a solution of 5.4 parts of chloromethyl pivalate in 60 parts of dry acetone were added 6 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt and 0.1 parts of sodium iodide. The mixture was refluxed for 10 hours, cooled and evaporated to an oil which was dissolved in diethyl ether. The ethereal solution was filtered and evaporated under vacuum to give an oil which solidified when triturated with petroleum ether (b.p. 40.degree.-60.degree. C). The solid was filtered off and crystallised from petroleum ether (b.p. 60.degree.-80.degree. C) to give 4.5 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid pivaloyoxymethyl ester, m.p. 79.degree.-80.degree. C.
Analysis Found: C, 66.70; H, 7.03%. C.sub.24 H.sub.30 O.sub.7 requires: C, 67.0; H, 6.98%.
The structure was confirmed by nuclear magnetic resonance and mass spectroscopy.
EXAMPLE 27
Bis-[8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid]2-oxapropan-1,3-diyl ester
To a suspension of 3 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt in 30 parts of dry acetone, were added 0.5 parts of 1,1-dichlorodimethyl ether and 0.1 parts of sodium iodide. The mixture was refluxed for 1 hour, cooled and filtered. The filtrate was evaporated to an oil which was dissolved in chloroform. The chloroform solution was filtered and evaporated. The resulting oil was triturated with ether to give a solid which was filtered off and crystallised from benzene/petroleum ether (b.p. 40.degree.-60.degree. C) to give 1 part of bis[8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2carboxylic acid]2-oxapropan-1,3-diyl ester, m.p. 120.degree.-121.degree. C.
Analysis Found: C, 67.20; H, 6.18%. C.sub.38 H.sub.42 O.sub.11 requires: C, 67.6; H, 6.20%.
The structure was confirmed by nuclear magnetic resonance and mass spectroscopy.
EXAMPLE 28
8-Allyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxamide
Anhydrous ammonia gas was passed through a stirred solution of 38.4 parts of 8-allyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid ethyl ester in 600 parts of anhydrous ethanol at 45.degree. C for 3 hours. The resulting dark, reddish brown solution was cooled to 0.degree. C. A white crystalline solid was formed, which was collected and dried in vacuo. 24.7 Parts of 8-allyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxamide were obtained, melting point 211.5.degree.-213.5.degree. C.
Analysis Found: C, 65.70; H, 5.84; N, 4.25%. C.sub.18 H.sub.19 NO.sub.5 requires: C, 65.64; H, 5.82; N, 4.255%.
Spectral confirmation
Molecular weight = 329 by mass spectrometry. The N.m.r. spectrum showed the 3-proton of the benzopyran ring as a singlet at 2.10.tau. and the exchangeable amide protons at 2.20.tau. in trifluoroacetic acid.
The infra-red spectrum displayed peaks at 3070 cm.sup.-.sup.1 for the 3-proton (C-H str) and 1715 and 1690 cm.sup.-.sup.1 for the 4-oxo (C=O str) and amide I band (C=O str) respectively.
EXAMPLE 29
The carboxylic acids shown in Table I were produced from the appropriate starting materials using the method of the Example Number quoted in the Table. The sodium salts of all of these acids were prepared by the process of Example 1 c).
EXAMPLE 30
The esters shown in Table II were prepared from the appropriate starting materials by the process shown in the table.
EXAMPLE 31
The amides, shown in Table III, were prepared from the corresponding ethyl esters by the method of Example 28.
EXAMPLE 32
The preparation of the acetophenone starting materials for many of the carboxylic acids and ethyl esters shown in Tables I and II are either given here in detail or are listed in Table IV.
a. 3-Ethyl-6-tetrahydrofurfuryloxy-2-hydroxyacetophenone
A mixture of 75 parts of 3-ethyl-2,6-dihydroxyacetophenone, 80 parts of tetrahydrofurfuryl methanesulphonate, 52 parts of potassium carbonate and 500 parts of N,N'-dimethylformamide was stirred and heated on a steam bath for 70 hours. The resulting dark solution was diluted with water and acidified with dilute hydrochloric acid. The precipitated oil was extracted with diethyl ether and the extract was evaporated to dryness under reduced pressure. A solution of the residual oil in diethyl ether was extracted with 5% aqueous sodium hydroxide solution. The alkaline solution was extracted with chloroform and the extract was washed with water, dried and evaporated. Petroleum ether (b.p. 40.degree.-60.degree. C) was added to the residue and the solution was decanted from some tarry material. Evaporation of the petrol solution gave 21 parts of 3-ethyl-6-tetrahydrofurfuryloxy-2-hydroxyacetophenone as an orange oil.
b. 3,5-Diallyl-2-hydroxyacetophenone
A mixture of 57.5 parts of 2-allyloxy-3-allylacetophenone and 45 parts of N,N-diethylaniline was heated in a sand bath for 4 hours at a temperature of 220.degree. C. After cooling, the reaction mixture was added to excess dilute hydrochloric acid and extracted with diethyl ether. The extract was extracted to afford a red oil which on distillation under vacuum gave 54 parts of 3,5-diallyl-2-hydroxyacetophenone, boiling point 100.degree.-120.degree. C at 1.2 mm mercury.
EXAMPLE 33
8-Allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid, 2'-dimethylamino-prop-2'-yl ester hydrochloride
To a suspension of 6.76 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid sodium salt in 200 parts of dry benzene were added 5.42 parts of 2-chloro-2-dimethyl-aminomethyl propane. The mixture was refluxed for 10 hours, cooled and filtered. The filtrate was evaporated to an oil which was dissolved in diethyl ether. The ethereal solution was filtered and evaporated to an oil which was then heated at 100.degree. C for 6 hours under vacuum.
After dissolving the oil in either an excess of ethereal hydrogen chloride solution was added. The resulting solid comprising 4 parts of 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H- 1-benzopyran-2-carboxylic acid, 2'-dimethylaminomethyl prop-2'-yl ester hydrochloride hydrate was filtered off, washed with ether and crystallised from benzene as a white solid m.p. 183.degree.-184.degree. C.
Analysis Found: C, 61.75; H, 8.04; N, 2.82%. C.sub.24 H.sub.34 ClNO.sub.5.H.sub.2 O requires: C, 61.4; H, 7.67; N, 2.98%.
The structure was confirmed by nuclear magnetic resonance and mass spectroscopy.
EXAMPLE 34
6,8-Di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid
A solution of 2.72 parts of 6,8-di-t-butyl-2-methyl-4-oxo-4H-1-benzopyran and 4.44 parts of selenium dioxide in a mixture of 60 parts of water and 250 parts by volume of dioxan was heated under gentle reflux for 12 hours. After cooling, the solution was filtered and the solvents were evaporated from the filtrate. The residue thus produced was dissolved in 250 parts by volume of chloroform and the resulting solution was extracted with 3 portions of 100 parts of a solution containing 5 parts of sodium bicarbonate in 100 parts of water. The combined aqueous washings were acidified with concentrated hydrochloric acid to give a solid which was crystallised from aqueous ethanol to give 6,8-di-t-butyl-4-oxo-4H-1-benzopyran-2-carboxylic acid, m.p. 230.degree.-232.degree. C.
EXAMPLE 35
6,8-Diethyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid
a. 3,5-Diethyl-2-hydroxy-6-methoxyacetophenone
A mixture of 43.2 parts of 3,5-diethyl-2,6-dihydroxyacetophenone, 30 parts of methyl iodide, 15.2 parts of anhydrous potassium carbonate and 300 parts of acetone was refluxed for two days. After cooling, the reaction mixture was filtered and the volatile components of the filtrate removed by evaporation. Chromatography of the residue obtained on silica gel, eluting with 4:1 and 2:1 mixtures of light petroleum and ether, gave 32 parts of 3,5-diethyl-2-hydroxy-6-methoxyacetophenone as an oil.
Spectral confirmation
Molecular weight = 222 by mass spectroscopy
C.sub.13 H.sub.18 O.sub.3 requires 222
b. 6,8-Diethyl-5-methoxy-4-oxo-4H-1-benzopyran-2-carboxylic acid
A solution of 13.8 parts of sodium in 300 parts of ethanol was added to a stirred solution of 32 parts of 3,5-diethyl-2-hydroxy-6-methoxyacetophenone and 99 parts by volume of diethyloxalate in 300 parts of ether. The reaction mixture was stirred at room temperature for 21/2 hours and then poured into a stirred mixture of 100 parts of chloroform, 300 parts of water and 60 parts of concentrated hydrochloric acid. The chloroform layer was separated and the solvent removed by evaporation to give an oil which was refluxed with a 95% v/v solution of ethanol containing concentrated sulphuric acid for four hours. Evaporation of the ethanol gave a residue which was refluxed with excess sodium bicarbonate solution for one hour. After cooling, the mixture was acidified with concentrated hydrochloric acid and extracted with chloroform. Evaporation of the chloroform and trituration of the residue obtained with light petroleum gave 17.6 parts of 6,8-diethyl-5-methoxy-4-oxo-4H-1-benzopyran-2-carboxylic acid, m.p. 199.degree.-200.degree. C (after crystallisation from chloroform/light petroleum).
Analysis Found: C, 64.4; H, 5.9%. C.sub.15 H.sub.16 O.sub.5 requires: C, 65.2; H, 5.8%.
Spectral confirmation
Molecular weight = 276 by mass spectroscopy.
C.sub.15 H.sub.16 O.sub.5 requires 276
c. 6,8-Diethyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid
A mixture of 5 parts of 6,8-diethyl-5-methoxy-4-oxo-4H-1-benzopyran-2-carboxylic acid and 130 parts by volume of 48% aqueous hydrobromic acid was refluxed for seven hours. The reaction mixture was cooled and excess sodium bicarbonate solution was added. The solution was acidified with concentrated hydrochloric acid and then extracted with chloroform. Evaporation of the chloroform gave a residue which on trituration with light petroleum gave 2.3 parts of 6,8-diethyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid, m.p. 218.degree.-220.degree. C (after crystallisation from ethanol).
Analysis Found: C, 63.8; H, 5.6%. C.sub.14 H.sub.14 C.sub.5 requires: C, 64.1; H, 53.3%.
Spectral confirmation
Molecular weight = 262 by mass spectroscopy
C.sub.14 H.sub.14 O.sub.5 requires 262
EXAMPLE A
The procedure set out below may be used to assess the effectiveness of a compound in inhibiting the release of the pharmacological mediators of anaphylaxis.
In this test, the effectiveness of the compounds in inhibiting the passive cutaneous anaphylactic reaction in rats is assessed. It has been proved that this form of test gives reliable qualitative indications of the ability of the compounds under test to inhibit antibody-antigen reactions in man.
In this test method Charles River France/Fisons bred rats (male or female) having a body weight of from 100 to 150 gms are infected subcutaneously at weekly intervals with N. brasiliensis larvae in doses increasing from about 2000 larvae per animal to 12,000 larvae per animal in order to establish the infection. After 8 weeks the rats are bled by heart puncture and 15-20 mls of blood are collected from each animal. The blood samples are then centrifuged at 3500 r.p.m. for 30 minutes in order to remove the blood cells from the blood plasma. The serum is collected and used to provide a serum containing N. brasiliensis antibody. A pilot sensitivity test is carried out to determine the least quantity of serum required to give a skin weal in control animals in the test described below of 2 cm diameter. It has been found that optimum sensitivity of rats in the body weight range 100-130 gms is obtained using a serum diluted with eight parts of physiological saline solution. This diluted solution is called antibody serum A.
The antigen to react with the antibody in serum A is prepared by removing N. brasiliensis worms from the gut of the infested rats, centrifuging the homogenate and collecting the supernatent liquor. This liquor is diluted with saline to give a protein content of 1 mg/ml and is known as solution B.
Charles River France/Fisons bred rats in the body weight ranges 100 to 130 gms are sensitised by intra dermal injection of 0.1 mls of serum A into the right flank. Sensitivity is allowed to develop for 24 hours and the rats are then injected intravenously with 1 ml/100 gms body weight of a mixture of solution B (0.25 mls), Evans Blue dye solution (0.25 mls) and the solution of the compound under test (0.25 mls with varying percentages of active matter). Insoluble compounds are administered as a separate intrapertioneal injection 5 minutes before intravenous administration of solution B and Evans Blue dye. For each percentage level of active matter in the solution under test five rats are injected. Five rats are used as controls in each test. The dosages of the compound under test are selected so as to give a range of inhibition values.
Thirty minutes after injection of solution B the rats are killed and the skins are removed and reversed. The intensity of the anaphylactic reaction is assessed by comparing the size of the characteristic blue weal produced by spread of the Evans Blue dye from the sensitisation site, with the size of the weal in the control animals. The size of the weal is rated as 0 (no weal detected, i.e., 100% inhibition) to 4 (no difference in size of weal, i.e., no inhibition) and the percentage inhibition for each dose level calculated as:
The percentage inhibitions for the various dose levels are plotted graphically for each compound. From these graphs the dosage required to achieve a 50% inhibition of the anaphylactic reaction (ID.sub.50) may be determined.
The compounds are also evaluated in the above manner using intestinal and gastric administration of the compound.
EXAMPLE B
Tablet
Compound of formula I, e.g. 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid,
Claims
We claim:
1. A composition useful for the prevention of asthmatic symptoms comprising an effective amount of a. 8-allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzo-pyran-2-carboxylic acid b. 5-(3-methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid c. 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid d. 6,8-diallyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid e. 7-(2-ethoxyethoxy)-6-hexyl-4-oxo-4H-1-benzopyran-2-carboxylic acid f. 8-ethyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid g. 8-allyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid h. 8-ethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid i. 6,8-diallyl-4-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid j. 6,8-diallyl-4-oxo-4H-1-benzopyran-2-carboxylic acid k. 5-(3-ethyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid l. 5-(3-ethyl-n-hexyloxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid m. 5-n-hexyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid n. 5-n-pentyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid o. 6,8-diethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid p. 6,8-dialllyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyuran-2-carboxylic acid q. 6,8-diethyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid r. 6,8-dimethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof as active ingredient, in combination with a pharmaceutically acceptable carrier.
2. The composition of claim 1 wherein the active ingredient is 8-allyl-5(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
3. The composition of claim 1 wherein the active ingredient is 5-(3-methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
4. The composition of claim 1 wherein the active ingredient is 8-allyl-5-(3-methyl-n-butoxy(-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
5. The composition of claim 1 wherein the active ingredient is 6,8-diallyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt therof.
6. The composition of claim 1 wherein the active ingredient is 7-(2-ethoxyethoxy)-6-hexyl-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
7. The composition of claim 1 wherein the active ingredient is 8-ethyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
8. The composition of claim 1 wherein the active ingredient is 8-allyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
9. The composition of claim 1 wherein the active ingredient is 8-ethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
10. The composition of claim 1 wherein the active ingredient is 6,8-diallyl-4-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
11. The composition of claim 1 wherein the active ingredient is 6,8-diallyl-4-oxo-4H-1-benzopyran-2-carboxylic acid of a pharmaceutically acceptable salt thereof.
12. The composition of claim 1 wherein the active ingredient is 5-(3-ethyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
13. The composition of claim 1 wherein the active ingredient is 5-(3-ethyl-n-hexyloxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
14. The composition of claim 1 wherein the active ingredient is 5-n-hexyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
15. The composition of claim 1 wherein the active ingredient is 5-n-pentyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
16. The composition of claim 1 wherein the active ingredient is 6,8-diethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
17. The composition of claim 1 wherein the active ingredient is 6,8-diallyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
18. The composition of claim 1 wherein the active ingredient is 6,8-diethyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
19. The composition of claim 1 wherein the active ingredient is 6,8-dimethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
20. A method of inhibiting the symptoms of asthma in a patient having asthma, which comprises administering to said patient an effective amount of a compound selected from the group consisting of a. 8-allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid b. 5-(3-methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid d. 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid d. 6,8-diallyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid e. 7-(2ethyoxyethoxy)-6-hexyl-4-oxo-4H-1-benzopyran-2-carboxylic acid f. 8-ethyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid g. 8-allyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid h. 8-ethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopryan-2-carboxylic acid i. 6,8-diallyl-4-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid j. 6,8-diallyl-4-oxo-4H-1-benzopyran-2-carboxylic acid k. 5-(3-ethyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid l. 5-(3-ethyl-n-hexyloxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid m. 5-n-hexyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid n. 5-n-pentyloxy-4-oxo-4H-1-benzopyran-2carboxylic acid o. 6,8-diethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid p. 6,8-diallyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid q. 6,8-diethyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid r. 6,8-dimethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2carboxylic acid or a pharmaceutically acceptable salt thereof.
21. The method of claim 1 wherein said compound is 8-allyl-5-(2-hydroxypropoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
22. The method of claim 21 wherein said compound is 5-(3-methyl-n-butoxy)-8-n-propyl-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
23. The method of claim 21 wherein said compound is 8-allyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
24. The method of claim 21 wherein said compound is 6,8-diallyl-5-hydroxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
25. The method of claim 21 wherein said compound is 7-(2-ethoxyethoxy)-6-hexyl-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
26. The method of claim 21 wherein said compound is 8-ethyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
27. The method of claim 21 wherein said compound is 8-allyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
28. The method of claim 21 wherein said compound is 8-ethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
29. The method of claim 21 wherein said compound is 6,8-diallyl-4-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
30. The method of claim 21 wherein said compound is 6,8-diallyl-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
31. The method of claim 21 wherein said compound is 5-(3-ethyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
32. The method of claim 21 wherein said compound is 5-(3-ethyl-n-hexyloxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
33. The method of claim 21 wherein said compound is 5-n-hexyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
34. The method of claim 21 wherein said compound is 5-n-pentyloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
35. The method of claim 21 wherein said compound is 6,8-diethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
36. The method of claim 21 wherein said compound is 6,8-diallyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
37. The method of claim 21 wherein said compound is 6,8-diethyl-5-tetrahydrofurfuryloxy-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
38. The method of claim 21 wherein said compound is 6,8-dimethyl-5-(3-methyl-n-butoxy)-4-oxo-4H-1-benzopyran-2-carboxylic acid or a pharmaceutically acceptable salt thereof.
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