ENGINEERED ADENO-ASSOCIATED VIRUS CAPSIDS

[0001] This application is a continuation of U.S. patent application Ser. No. 17/642,541, filed on Mar. 11, 2022, which is the U.S. National Stage Application under 35 U.S.C. § 371 of Patent Cooperation Treaty Application No.: PCT/US2020/050534, filed on Sep. 11, 2020. Patent Cooperation Treaty Application No.: PCT/US2020/050534 claims the benefit of and priority to U.S. Provisional Patent Application No. 62/899,453, filed on Sep. 12, 2019, and U.S. Provisional Patent Application No. 62/916,185, filed on Oct. 16, 2019. The entire contents of the above-identified applications are hereby fully incorporated herein by reference.

[0002] This application contains an electronic sequence listing entitled BROD-4400US-CON2_ST26, created on Nov. 14, 2025, and having a size of 10,195,472 bytes. The content of the sequence listing is incorporated herein in its entirety.

[0003] The subject matter disclosed herein is generally directed to recombinant adeno-associated virus (AAV) vectors and systems thereof, compositions, and uses thereof.

[0004] Recombinant AAVs (rAAVs) are the most commonly used delivery vehicles for gene therapy and gene editing. Nonetheless, rAAVs that contain natural capsid variants have limited cell tropism. Indeed, rAA Vs used today mainly infect the liver after systemic delivery. Further, the transduction efficiency of conventional rAAVs in other cell-types, tissues, and organs by these conventional rAAVs with natural capsid variants is limited. Therefore, AAV-mediated polynucleotide delivery for diseased that affect cells, tissues, and organs other than the liver (e.g. nervous system, skeletal muscle, and cardiac muscle) typically requires an injection of a large dose of virus (typically about 1×10¹⁴ vg/kg), which often results in liver toxicity. Furthermore, because large doses are required when using conventional rAAVs, manufacturing sufficient amounts of a therapeutic rAAV needed to dose adult patients is extremely challenging. Additionally, due to differences in gene expression and physiology, mouse and primate models respond differently to viral capsids. Transduction efficiency of different virus particles varies between different species, and as a result, preclinical studies in mice often do not accurately reflect results in primates, including humans. As such there exists a need for improved rAAVs for use in the treatment of various genetic diseases.

[0005] In certain example embodiments, provided herein are various embodiments of engineered adeno-associated virus (AAV) capsids that can be engineered to confer cell-specific tropism to an engineered AAV particle. The engineered capsids can be included in an engineered virus particle and can confer cell-specific tropism, reduced immunogenicity, or both to the engineered AAV particle. The engineered AAV capsids described herein can include one or more engineered AAV capsid proteins described herein. The engineered AAV capsid and/or capsid proteins can be encoded by one or more engineered AAV capsid polynucleotides. In some embodiments, an engineered AAV capsid polynucleotide can include a 3′ polyadenylation signal. The polyadenylation signal can be an SV40 polyadenylation signal. In some embodiments, the engineered AAV capsid protein can have an n-mer amino acid motif, where n can be at least 3 amino acids. In some embodiments, n can be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 amino acids.

[0006] In certain example embodiments, also provided herein are methods of generating engineered AAV capsids. In some embodiments, the method of generating an AAV capsid variant can include the steps of (a) expressing a vector system described herein that contains an engineered AAV capsid polynucleotide in a cell to produce engineered AAV virus particle capsid variants; (b) harvesting the engineered AAV virus particle capsid variants produced in step (a); (c) administering engineered AAV virus particle capsid variants to one or more first subjects, wherein the engineered AAV virus particle capsid variants are produced by expressing an engineered AAV capsid variant vector or system thereof in a cell and harvesting the engineered AAV virus particle capsid variants produced by the cell; and (d) identifying one or more engineered AAV virus particle capsid particle variants produced at a significantly high level by one or more specific cells or specific cell types in the one or more first subjects. The method can further include the steps of (e) administering some or all engineered AAV virus particle capsid variants identified in step (d) to one or more second subjects; and (f) identifying one or more engineered AAV virus particle capsid variants produced at a significantly high level in one or more specific cells or specific cell types in the one or more second subjects. The cell in step (a) can be a prokaryotic cell or a eukaryotic cell. In some embodiments, the administration in step (c), step (e), or both is systemic. In some embodiments, one or more first subjects, one or more second subjects, or both, are non-human mammals. In some embodiments, one or more first subjects, one or more second subjects, or both, are each independently selected from the group consisting of a wild-type non-human mammal, a humanized non-human mammal, a disease-specific non-human mammal model, and a non-human primate.

[0007] In certain example embodiments, also provided herein are vectors and vector systems that can contain one or more of the engineered AAV capsid polynucleotides described herein. As used in this context, engineered AAV capsid polynucleotides refers to any one or more of the polynucleotides described herein capable of encoding an engineered AAV capsid as described elsewhere herein and/or polynucleotide(s) capable of encoding one or more engineered AAV capsid proteins described elsewhere herein. Further, where the vector includes an engineered AAV capsid polynucleotide described herein, the vector can also be referred to and considered an engineered vector or system thereof although not specifically noted as such. In embodiments, the vector can contain one or more polynucleotides encoding one or more elements of an engineered AAV capsid described herein. In some embodiments, one or more of the polynucleotides that are part of the engineered AAV capsid and system thereof described herein can be included in a vector or vector system.

[0008] In certain example embodiments, the vector can include an engineered AAV capsid polynucleotide having a 3′ polyadenylation signal. In some embodiments, 3′ polyadenylation is an SV40 polyadenylation signal. In some embodiments, the vector does not have splice regulatory elements. In some embodiments, the vector includes one or more minimal splice regulatory elements. In some embodiments, the vector can further include a modified splice regulatory element, wherein the modification inactivates the splice regulatory element. In some embodiments, the modified splice regulatory element is a polynucleotide sequence sufficient to induce splicing between a rep protein polynucleotide and the engineered AAV capsid protein variant polynucleotide. In some embodiments, the polynucleotide sequence can be sufficient to induce splicing is a splice acceptor or a splice donor. In some embodiments, the AAV capsid polynucleotide is an engineered AAV capsid polynucleotide as described elsewhere herein. In some exemplary embodiments, the vectors and/or vector systems can be used, for example, to express one or more of the engineered AAV capsid polynucleotides in a cell, such as a producer cell, to produce engineered AAV particles containing an engineered AAV capsid described elsewhere herein.

[0009] In certain example embodiments, also provided herein are engineered AAV capsid virus particles that can contain an engineered AAV capsid as described in detail elsewhere herein. An engineered AAV capsid is one that contains one or more engineered AAV capsid proteins as are described elsewhere herein. In some embodiments, the engineered AAV particles can include 1-60 engineered AAV capsid proteins described herein. In some embodiments, the engineered AAV capsid can confer a cell-cell specific tropism, reduce immunogenicity, or both to the engineered AAV capsid virus particle. The engineered AAV capsid virus particle can include one or more cargo polynucleotides. In some embodiments, the engineered AAV capsid virus particle described herein can be used to deliver a cargo polynucleotide to a cell. In some embodiments, the cargo polynucleotide is a gene modification polynucleotide. In some embodiments, the cargo polynucleotide is a component or encodes a component of a CRISPR-Cas system.

[0010] In certain example embodiments, also provided herein are engineered cells that can include one or more of the engineered AAV capsid polynucleotides, polypeptides, vectors, and/or vector systems. In some embodiments, one or more of the engineered AAV capsid polynucleotides can be expressed in the engineered cells. In some embodiments, the engineered cells can be capable of producing engineered AAV capsid proteins and/or engineered AAV capsid particles that are described elsewhere herein.

[0011] In certain example embodiments, also provided herein are modified or engineered organisms that can include one or more engineered cells described herein.

[0012] In certain example embodiments, component(s) of the engineered AAV capsid system, engineered cells, engineered AAV capsid particles, and/or combinations thereof can be included in a formulation that can be delivered to a subject or a cell. In certain example embodiments, also provided herein are pharmaceutical formulations containing an amount of one or more of the engineered AAV capsid polypeptides, polynucleotides, vectors, cells, or combinations thereof described herein.

[0013] In certain example embodiments, also provided herein are kits that contain one or more of the one or more of the engineered AAV capsid polypeptides, polynucleotides, vectors, cells, or other components described herein, or a combination thereof, or one or more pharmaceutical formulations described herein. In some exemplary embodiments, one or more of the polypeptides, polynucleotides, vectors, cells, and combinations thereof described herein can be presented as a combination kit.

[0014] In certain example embodiments, provided herein are methods of using the engineered AAV capsid variants, virus particles, cells and formulations thereof. In some exemplary embodiments, the engineered AAV capsid system polynucleotides, polypeptides, vector(s), engineered cells, engineered AAV capsid particles can be used generally to package and/or deliver one or more cargo polynucleotides to a recipient cell. In some exemplary embodiments, delivery is done in cell-specific manner based upon the tropism of the engineered AAV capsid.

[0015] In some exemplary embodiments, provided herein are methods of using the engineered AAV capsid polynucleotides, vectors, and systems thereof to generate engineered AAV capsid variant libraries that can be mined for variants with a desired cell-specificity.

[0016] In some exemplary embodiments, provided herein are methods using the engineered AAV capsid variants to deliver a therapeutic cargo polynucleotide to a subject in need thereof. In some embodiments, the therapeutic cargo polynucleotide can be and/or encode a component of a CRISPR-Cas system. In some embodiments, the subject in need thereof can have a disease having a genetic or epigenetic embodiments. In some embodiments, the subject in need thereof can have a muscle disease.

[0017] In some exemplary embodiments, provided herein are methods of using the engineered AAV capsid virus particles to deliver a cargo polynucleotide capable of modifying a recipient cell to the recipient cell for use in adoptive cell therapy. In some exemplary embodiments, the recipient cell is a T cell. In some exemplary embodiments, the recipient cell is a B cell. In some exemplary embodiments, the cell is a CAR T cell.

[0018] In some exemplary embodiments, provided herein are methods of using the engineered AAV capsid virus particles to deliver a cargo polynucleotide capable of modifying a recipient cell to create a gene drive in the recipient cell.

[0019] In some exemplary embodiments, provided herein are methods of using the engineered AAV capsid virus particles to deliver a cargo polynucleotide capable of modifying recipient cells, tissues, and/or organs for transplantation.

[0020] Described in certain example embodiments herein are vectors comprising: an adeno-associated (AAV) capsid protein polynucleotide, wherein the AAV capsid protein polynucleotide comprises a 3′ polyadenylation signal.

[0021] In certain example embodiments, the vector does not comprise splice regulatory elements.

[0022] In certain example embodiments, the vector comprises minimal splice regulatory elements.

[0023] In certain example embodiments, the vector further comprises a modified splice regulatory element, wherein the modification inactivates the splice regulatory element.

[0024] In certain example embodiments, the modified splice regulatory element is a polynucleotide sequence sufficient to induce splicing, between a rep protein polynucleotide and the capsid protein polynucleotide.

[0025] In certain example embodiments, the polynucleotide sequence sufficient to induce splicing is a splice acceptor or a splice donor.

[0026] In certain example embodiments, the polyadenylation signal is an SV40 polyadenylation signal.

[0027] In certain example embodiments, the AAV capsid polynucleotide is an engineered AAV capsid polynucleotide.

[0028] In certain example embodiments, the engineered AAV capsid polynucleotide comprises a n-mer motif polynucleotide capable of encoding an n-mer amino acid motif, wherein the n-mer motif comprises three or more amino acids, wherein the n-mer motif polynucleotide is inserted between two codons in the AAV capsid polynucleotide within a region of the AAV capsid polynucleotide capable of encoding a capsid surface.

[0029] In certain example embodiments, the n-mer motif comprises 3-15 amino acids.

[0030] In certain example embodiments, the n-mer motif is 6 or 7 amino acids.

[0031] In certain example embodiments, the n-mer motif polynucleotide is inserted between the codons corresponding to any two contiguous amino acids between amino acids 262-269, 327-332, 382-386, 452-460, 488-505, 527-539, 545-558, 581-593, 704-714, or any combination thereof in an AAV9 capsid polynucleotide or in an analogous position in an AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8 capsid polynucleotide.

[0032] In certain example embodiments, the n-mer motif polynucleotide is inserted between the codons corresponding to aa588 and 589 in the AAV9 capsid polynucleotide.

[0033] In certain example embodiments, the vector is capable of producing AAV virus particles having increased specificity, reduced immunogenicity, or both.

[0034] In certain example embodiments, the vector is capable of producing AAV virus particles having increased muscle cell, specificity, reduced immunogenicity, or both.

[0035] In certain example embodiments, the n-mer motif polynucleotide is any polynucleotide in any of Tables 1-6.

[0036] In certain example embodiments, the n-mer motif polynucleotide is capable of encoding a peptide as in any of Tables 1-6.

[0037] In certain example embodiments, the n-mer motif polynucleotide is capable of encoding three or more amino acids, wherein the first three amino acids are RGD.

[0038] In certain example embodiments, the n-mer motif has a polypeptide sequence of RGD or RGDX_n, where n is 3-15 amino acids and X, where each amino acid present are independently selected from the others from the group of any amino acid.

[0039] In certain example embodiments, the vector is capable of producing an AAV capsid polypeptide, AAV capsid, or both that have a muscle-specific tropism.

[0040] Described in certain example embodiments herein are vector systems comprising: a vector as described elsewhere herein; an AAV rep protein polynucleotide or portion thereof; and a single promoter operably coupled to the AAV capsid protein, AAV rep protein, or both, wherein the single promoter is the only promoter operably coupled to the AAV capsid protein, AAV rep protein, or both.

[0041] Described in certain example embodiments herein, are vector systems comprising a vector as described herein; and an AAV rep protein polynucleotide or portion thereof.

[0042] In certain example embodiments, the vector system further comprises a first promoter, wherein the first promoter is operably coupled to the AAV capsid protein, AAV rep protein, or both.

[0043] In certain example embodiments, the first promoter or the single promoter is a cell-specific promoter.

[0044] In certain example embodiments, the first promoter is capable of driving high-titer viral production in the absence of an endogenous AAV promoter.

[0045] In certain example embodiments, the endogenous AAV promoter is p40.

[0046] In certain example embodiments, the AAV rep protein polynucleotide is operably coupled to the AAV capsid protein.

[0047] In certain example embodiments, the AAV protein polynucleotide is part of the same vector as the AAV capsid protein polynucleotide.

[0048] In certain example embodiments, the AAV protein polynucleotide is on a different vector as the AAV capsid protein polynucleotide.

[0049] Described in example embodiments herein are polypeptides encoded by a vector as described herein or by a vector system as described herein.

[0050] Described in example embodiments herein are cells comprising: a vector as described herein, a vector system as described herein, a polypeptide as described herein, or any combination thereof.

[0051] In certain example embodiments, the cell is prokaryotic.

[0052] In certain example embodiments, the cell is eukaryotic.

[0053] Described in certain example embodiments herein are engineered adeno-associated virus particle produced by the method comprising: expressing a vector as described herein, a vector system as described herein, or both in a cell.

[0054] In certain example embodiments, the step of expressing the vector system occurs in vitro or ex vivo.

[0055] In certain example embodiments, the step of expressing the vector system occurs in vivo.

[0056] Described in certain example embodiments herein are methods of identifying cell-specific adeno-associated virus (AAV) capsid variants, comprising:

• • [0057] (a) expressing a vector system as described herein in a cell to produce AAV engineered virus particle capsid variants;
  • [0058] (b) harvesting the engineered AAV virus particle capsid variants produced in step (a);
  • [0059] (c) administering engineered AAV virus particle capsid variants to one or more first subjects, wherein the engineered AAV virus particle capsid variants are produced by expressing a vector system as described herein in a cell and harvesting the engineered AAV virus particle capsid variants produced by the cell; and
  • [0060] (d) identifying one or more engineered AAV capsid variants produced at a significantly high level by one or more specific cells or specific cell types in the one or more first subjects.

[0061] In certain example embodiments, the method further comprises

• • [0062] (e) administering some or all engineered AAV virus particle capsid variants identified in step (d) to one or more second subjects; and
  • [0063] (f) identifying one or more engineered AAV virus particle capsid variants produced at a significantly high level in one or more specific cells or specific cell types in the one or more second subjects.

[0064] In certain example embodiments, the cell is a prokaryotic cell.

[0065] In certain example embodiments, cell is a eukaryotic cell.

[0066] In certain example embodiments, administration in step (c), step (e), or both is systemic.

[0067] In certain example embodiments, the one or more first subjects, one or more second subjects, or both, are non-human mammals.

[0068] In certain example embodiments, the one or more first subjects, one or more second subjects, or both, are each independently selected from the group consisting of: a wild-type non-human mammal, a humanized non-human mammal, a disease-specific non-human mammal model, and a non-human primate.

[0069] Described in certain example embodiments herein are vector systems comprising a vector comprising a cell-specific capsid polynucleotide, wherein the cell-specific capsid polynucleotide encodes a cell-specific capsid protein; and optionally, a regulatory element operatively coupled to the cell-specific capsid polynucleotide.

[0070] In certain example embodiments herein, the cell-specific capsid polynucleotide is identified by a method as described herein and as further described elsewhere herein.

[0071] In certain example embodiments, the vector system further comprises a cargo.

[0072] In certain example embodiments, the cargo is a cargo polynucleotide encodes a gene-modification molecule, a non-gene modification polypeptide, a non-gene modification RNA, or a combination thereof.

[0073] In certain example embodiments, the cargo polynucleotide is present on the same vector or a different vector than the cell-specific capsid polynucleotide.

[0074] In certain example embodiments, the vector system is capable of producing a cell-specific capsid polynucleotide and/or polypeptide.

[0075] In certain example embodiments, the cell-specific capsid polynucleotide is a cell-specific adeno-associated virus (AAV) capsid polynucleotide that encodes a cell-specific AAV capsid polypeptide.

[0076] In certain example embodiments, the vector system is capable of producing virus particles comprising the cell-specific capsid protein and that further comprise the cargo when present.

[0077] In certain example embodiments, the viral particles are AAV viral particles.

[0078] In certain example embodiments, the viral particles are engineered AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV rh.74, or AAV rh.10 viral particles.

[0079] In certain example embodiments, the cell-specific viral capsid polypeptide is a cell-specific AAV capsid polypeptide.

[0080] In certain example embodiments, the cell-specific AAV capsid polypeptide is an engineered AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV rh.74, or AAV rh.10 capsid polypeptide.

[0081] In certain example embodiments, the cell-specific capsid polynucleotide does not comprise splice regulatory elements.

[0082] In certain example embodiments, the vector further comprises a viral rep protein.

[0083] In certain example embodiments, the viral rep protein is an AAV viral rep protein.

[0084] In certain example embodiments, the viral rep protein is on the same vector as or a different vector from the cell-specific capsid polynucleotide.

[0085] In certain example embodiments, the viral rep protein is operatively coupled to a regulatory element.

[0086] Described in certain example embodiments herein are polypeptides that are produced by the vector system described herein.

[0087] Described in certain example embodiments herein are cells comprising the vector system or the polypeptide described herein.

[0088] In certain example embodiments, the cell is a prokaryotic.

[0089] In certain example embodiments, the cell is a eukaryotic cell.

[0090] Described in certain example embodiments herein are engineered virus particles comprising: a cell-specific capsid, wherein the cell-specific capsid is encoded by a cell-specific capsid polynucleotide of the vector system described herein.

[0091] In certain example embodiments, the engineered virus particle further comprises a cargo molecule, wherein the cargo molecule is encoded by a cargo polynucleotide of the vector system described herein.

[0092] In certain example embodiments, the cargo molecule is a gene modification molecule, a non-gene modification polypeptide, a non-gene modification RNA, or a combination thereof.

[0093] In certain example embodiments, the engineered virus particle is an engineered adeno-associated virus particle.

[0094] Described in certain example embodiments herein are engineered virus particles produced by the method comprising: expressing a vector system described herein in a cell.

[0095] Described in certain example embodiments herein are pharmaceutical formulations comprising: a vector system as described herein, a polypeptide as described herein, a cell as described herein, an engineered virus particle as described herein, or a combination thereof; and a pharmaceutically acceptable carrier.

[0096] Described in certain example embodiments herein are methods comprising administering a vector system as described herein, a polypeptide as described herein, a cell as described herein, an engineered virus particle as described herein, a pharmaceutical formulation as described herein, or a combination thereof to a subject.

[0097] These and other embodiments, objects, features, and advantages of the example embodiments will become apparent to those having ordinary skill in the art upon consideration of the following detailed description of illustrated example embodiments.

[0120] The figures herein are for illustrative purposes only and are not necessarily drawn to scale.

[0121] Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure pertains. Definitions of common terms and techniques in molecular biology may be found in Molecular Cloning: A Laboratory Manual, 2^nd edition (1989) (Sambrook, Fritsch, and Maniatis); Molecular Cloning: A Laboratory Manual, 4^th edition (2012) (Green and Sambrook); Current Protocols in Molecular Biology (1987) (F. M. Ausubel et al. eds.); the series Methods in Enzymology (Academic Press, Inc.): PCR 2: A Practical Approach (1995) (M. J. MacPherson, B. D. Hames, and G. R. Taylor eds.): Antibodies, A Laboratory Manual (1988) (Harlow and Lane, eds.): Antibodies A Laboratory Manual, 2^nd edition 2013 (E. A. Greenfield ed.); Animal Cell Culture (1987) (R. I. Freshney, ed.); Benjamin Lewin, Genes IX, published by Jones and Bartlett, 2008 (ISBN 0763752223); Kendrew et al. (eds.), The Encyclopedia of Molecular Biology, published by Blackwell Science Ltd., 1994 (ISBN 0632021829); Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference, published by VCH Publishers, Inc., 1995 (ISBN 9780471185710); Singleton et al., Dictionary of Microbiology and Molecular Biology 2^nd ed., J. Wiley & Sons (New York, N.Y. 1994), March, Advanced Organic Chemistry Reactions, Mechanisms and Structure 4^th ed., John Wiley & Sons (New York, N.Y. 1992); and Marten H. Hofker and Jan van Deursen, Transgenic Mouse Methods and Protocols, 2^nd edition (2011)

[0122] As used herein, the singular forms “a”, “an”, and “the” include both singular and plural referents unless the context clearly dictates otherwise.

[0123] The term “optional” or “optionally” means that the subsequent described event, circumstance or substituent may or may not occur, and that the description includes instances where the event or circumstance occurs and instances where it does not.

[0124] The recitation of numerical ranges by endpoints includes all numbers and fractions subsumed within the respective ranges, as well as the recited endpoints. It will be further understood that the endpoints of each of the ranges are significant both in relation to the other endpoint, and independently of the other endpoint. It is also understood that there are a number of values disclosed herein, and that each value is also herein disclosed as “about” that particular value in addition to the value itself. For example, if the value “10” is disclosed, then “about 10” is also disclosed. Ranges can be expressed herein as from “about” one particular value, and/or to “about” another particular value. Similarly, when values are expressed as approximations, by use of the antecedent “about,” it will be understood that the particular value forms a further embodiment. For example, if the value “about 10” is disclosed, then “10” is also disclosed.

[0125] It is to be understood that such a range format is used for convenience and brevity, and thus, should be interpreted in a flexible manner to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited. To illustrate, a numerical range of “about 0.1% to 5%” should be interpreted to include not only the explicitly recited values of about 0.1% to about 5%, but also include individual values (e.g., about 1%, about 2%, about 3%, and about 4%) and the sub-ranges (e.g., about 0.5% to about 1.1%; about 5% to about 2.4%; about 0.5% to about 3.2%, and about 0.5% to about 4.4%, and other possible sub-ranges) within the indicated range. Where a range is expressed, a further embodiment includes from the one particular value and/or to the other particular value.

[0126] Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limit of that range and any other stated or intervening value in that stated range, is encompassed within the disclosure. The upper and lower limits of these smaller ranges may independently be included in the smaller ranges and are also encompassed within the disclosure, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the disclosure. For example, where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the disclosure, e.g. the phrase “x to y” includes the range from ‘x’ to ‘y’ as well as the range greater than ‘x’ and less than ‘y’. The range can also be expressed as an upper limit, e.g. ‘about x, y, z, or less’ and should be interpreted to include the specific ranges of ‘about x’, ‘about y’, and ‘about z’ as well as the ranges of ‘less than x’, less than y′, and ‘less than z’. Likewise, the phrase ‘about x, y, z, or greater’ should be interpreted to include the specific ranges of ‘about x’, ‘about y’, and ‘about z’ as well as the ranges of ‘greater than x’, greater than y′, and ‘greater than z’. In addition, the phrase “about ‘x’ to ‘y’”, where ‘x’ and ‘y’ are numerical values, includes “about ‘x’ to about ‘y’”.

[0127] The terms “about” or “approximately” as used herein when referring to a measurable value such as a parameter, an amount, a temporal duration, and the like, are meant to encompass variations of and from the specified value, such as variations of +/−10% or less, +/−5% or less, +/−1% or less, and +/−0.1% or less of and from the specified value, insofar such variations are appropriate to perform in the disclosed invention. It is to be understood that the value to which the modifier “about” or “approximately” refers is itself also specifically, and preferably, disclosed. As used herein, the terms “about,” “approximate,” “at or about,” and “substantially” can mean that the amount or value in question can be the exact value or a value that provides equivalent results or effects as recited in the claims or taught herein. That is, it is understood that amounts, sizes, formulations, parameters, and other quantities and characteristics are not and need not be exact, but may be approximate and/or larger or smaller, as desired, reflecting tolerances, conversion factors, rounding off, measurement error and the like, and other factors known to those of skill in the art such that equivalent results or effects are obtained. In some circumstances, the value that provides equivalent results or effects cannot be reasonably determined. In general, an amount, size, formulation, parameter or other quantity or characteristic is “about,” “approximate,” or “at or about” whether or not expressly stated to be such. It is understood that where “about,” “approximate,” or “at or about” is used before a quantitative value, the parameter also includes the specific quantitative value itself, unless specifically stated otherwise.

[0128] As used herein, a “biological sample” may contain whole cells and/or live cells and/or cell debris. The biological sample may contain (or be derived from) a “bodily fluid”. The present invention encompasses embodiments wherein the bodily fluid is selected from amniotic fluid, aqueous humour, vitreous humour, bile, blood serum, breast milk, cerebrospinal fluid, cerumen (earwax), chyle, chyme, endolymph, perilymph, exudates, feces, female ejaculate, gastric acid, gastric juice, lymph, mucus (including nasal drainage and phlegm), pericardial fluid, peritoneal fluid, pleural fluid, pus, rheum, saliva, sebum (skin oil), semen, sputum, synovial fluid, sweat, tears, urine, vaginal secretion, vomit and mixtures of one or more thereof. Biological samples include cell cultures, bodily fluids, cell cultures from bodily fluids. Bodily fluids may be obtained from a mammal organism, for example by puncture, or other collecting or sampling procedures.

[0129] The terms “subject,” “individual,” and “patient” are used interchangeably herein to refer to a vertebrate, preferably a mammal, more preferably a human. Mammals include, but are not limited to, murines, simians, humans, farm animals, sport animals, and pets. Tissues, cells and their progeny of a biological entity obtained in vivo or cultured in vitro are also encompassed.

[0130] Various embodiments are described hereinafter. It should be noted that the specific embodiments are not intended as an exhaustive description or as a limitation to the broader embodiments discussed herein. One embodiment described in conjunction with a particular embodiment is not necessarily limited to that embodiment and can be practiced with any other embodiment(s). Reference throughout this specification to “one embodiment”, “an embodiment,” “an example embodiment,” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, appearances of the phrases “in one embodiment,” “in an embodiment,” or “an example embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment, but may. Furthermore, the particular features, structures or characteristics may be combined in any suitable manner, as would be apparent to a person skilled in the art from this disclosure, in one or more embodiments. Furthermore, while some embodiments described herein include some, but not other features included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the invention. For example, in the appended claims, any of the claimed embodiments can be used in any combination.

[0131] All publications, published patent documents, and patent applications cited herein are hereby incorporated by reference to the same extent as though each individual publication, published patent document, or patent application was specifically and individually indicated as being incorporated by reference.

[0132] Embodiments disclosed herein provide engineered adeno-associated virus (AAV) capsids that can be engineered to confer cell-specific and/or species-specific tropism to an engineered AAV particle.

[0133] Embodiments disclosed herein also provide methods of generating the rAAVs having engineered capsids that can involve systematically directing the generation of diverse libraries of variants of modified surface structures, such as variant capsid proteins. Embodiments of the method of generating rAAVs having engineered capsids can also include stringent selection of capsid variants capable of targeting a specific cell, tissue, and/or organ type. Embodiments of the method of generating rAA Vs having engineered capsids can include stringent selection of capsid variants capable of efficient and/or homogenous transduction in at least two or more species.

[0134] Embodiments disclosed herein provide vectors and systems thereof capable of producing an engineered AAV described herein.

[0135] Embodiments disclosed herein provide cells that can be capable of producing the engineered AAV particles described herein. In some embodiments, the cells include one or more vectors or system thereof described herein.

[0136] Embodiments disclosed herein provide engineered AAVs that can include an engineered capsid described herein. In some embodiments, the engineered AAV can include a cargo polynucleotide to be delivered to a cell. In some embodiments, the cargo polynucleotide is a gene modification polynucleotide.

[0137] Embodiments disclosed herein provide formulations that can contain an engineered AAV vector or system thereof, an engineered AAV capsid, engineered AAV particles including an engineered AAV capsid described herein, and/or an engineered cell described herein that contains an engineered AAV capsid, and/or an engineered AAV vector or system thereof. In some embodiments, the formulation can also include a pharmaceutically acceptable carrier. The formulations described herein can be delivered to a subject in need thereof or a cell.

[0138] Embodiments disclosed herein also provide kits that contain one or more of the one or more of the polypeptides, polynucleotides, vectors, engineered AAV capsids, engineered AAV particles, cells, or other components described herein and combinations thereof and pharmaceutical formulations described herein. In embodiments, one or more of the polypeptides, polynucleotides, vectors, engineered AAV capsids, engineered AAV particles cells, and combinations thereof described herein can be presented as a combination kit.

[0139] Embodiments disclosed herein provide methods of using the engineered AAVs having a cell-specific tropism described herein to deliver, for example, a therapeutic polynucleotide to a cell. In this way, the engineered AA Vs described herein can be used to treat and/or prevent a disease in a subject in need thereof. Embodiments disclosed herein also provide methods of delivering the engineered AAV capsids, engineered AAV virus particles, engineered AAV vectors or systems thereof and/or formulations thereof to a cell. Also provided herein are methods of treating a subject in need thereof by delivering an engineered AAV particle, engineered AAV capsid, engineered AAV capsid vector or system thereof, an engineered cell, and/or formulation thereof to the subject.

[0140] Additional features and advantages of the embodiments engineered AAVs and methods of making and using the engineered AAVs are further described herein.

[0141] Described herein are various embodiments of engineered adeno-associated virus (AAV) capsids that can be engineered to confer cell-specific tropism to an engineered AAV particle. The engineered capsids can be included in an engineered virus particle, and can confer cell-specific tropism, reduced immunogenicity, or both to the engineered AAV particle. The engineered AAV capsids described herein can include one or more engineered AAV capsid proteins described herein.

[0142] The engineered AAV capsid and/or capsid proteins can be encoded by one or more engineered AAV capsid polynucleotides. In some embodiments, an engineered AAV capsid polynucleotide can include a 3′ polyadenylation signal. The polyadenylation signal can be an SV40 polyadenylation signal.

[0143] The engineered AAV capsids can be variants of wild-type AAV capsids. In some embodiments, the wild-type AAV capsids can be composed of VP1, VP2, VP3 capsid proteins or a combination thereof. In other words, the engineered AAV capsids can include one or more variants of a wild-type VP1, wild-type VP2, and/or wild-type VP3 capsid proteins. In some embodiments, the serotype of the reference wild-type AAV capsid can be AAV-1, AAV-2, AAV-3, AAV-4, AAV-5, AAV-6, AAV-8, AAV-9 or any combination thereof. In some embodiments, the serotype of the wild-type AAV capsid can be AAV-9. The engineered AAV capsids can have a different tropism than that of the reference wild-type AAV capsid.

[0144] The engineered AAV capsid can contain 1-60 engineered capsid proteins. In some embodiments, the engineered AAV capsids can contain 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, or 60 engineered capsid proteins. In some embodiments, the engineered AAV capsid can contain 0-59 wild-type AAV capsid proteins. In some embodiments, the engineered AAV capsid can contain 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, or 59 wild-type AAV capsid proteins.

[0145] In some embodiments, the engineered AAV capsid protein can have an n-mer amino acid motif, where n can be at least 3 amino acids. In some embodiments, n can be 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 amino acids. In some embodiments, the engineered AAV capsid can have a 6-mer or 7-mer amino acid motif. In some embodiments, the n-mer amino acid motif can be inserted between two amino acids in the wild-type viral protein (VP) (or capsid protein). In some embodiments, the n-mer motif can be inserted between two amino acids in a variable amino acid region in an AAV capsid protein. The core of each wild-type AAV viral protein contains an eight-stranded beta-barrel motif (betaB to betaI) and an alpha-helix (alphaA) that are conserved in autonomous parvovirus capsids (see e.g. DiMattia et al. 2012. J. Virol. 86 (12): 6947-6958). Structural variable regions (VRs) occur in the surface loops that connect the beta-strands, which cluster to produce local variations in the capsid surface. AAVs have 12 variable regions (also referred to as hypervariable regions) (see e.g. Weitzman and Linden. 2011. “Adeno-Associated Virus Biology.” In Snyder, R. O., Moullier, P. (eds.) Totowa, NJ: Humana Press). In some embodiments, one or more n-mer motifs can be inserted between two amino acids in one or more of the 12 variable regions in the wild-type AVV capsid proteins. In some embodiments, the one or more n-mer motifs can be each be inserted between two amino acids in VR-I, VR-II, VR-III, VR-IV, VR-V, VR-VI, VR-VII, VR-III, VR-IX, VR-X, VR-XI, VR-XII, or a combination thereof. In some embodiments, the n-mer can be inserted between two amino acids in the VR-III of a capsid protein. In some embodiments, the engineered capsid can have an n-mer inserted between any two contiguous amino acids between amino acids 262 and 269, between any two contiguous amino acids between amino acids 327 and 332, between any two contiguous amino acids between amino acids 382 and 386, between any two contiguous amino acids between amino acids 452 and 460, between any two contiguous amino acids between amino acids 488 and 505, between any two contiguous amino acids between amino acids 545 and 558, between any two contiguous amino acids between amino acids 581 and 593, between any two contiguous amino acids between amino acids 704 and 714 of an AAV9 viral protein. In some embodiments, the engineered capsid can have an n-mer inserted between amino acids 588 and 589 of an AAV9 viral protein. In some embodiments, the engineered capsid can have a 7-mer motif inserted between amino acids 588 and 589 of an AAV9 viral protein. SEQ ID NO: 1 is a reference AAV9 capsid sequence for at least referencing the insertion sites discussed above. It will be appreciated that n-mers can be inserted in analogous positions in AAV viral proteins of other serotypes. In some embodiments as previously discussed, the n-mer(s) can be inserted between any two contiguous amino acids within the AAV viral protein and in some embodiments the insertion is made in a variable region.

AAV9 capsid reference Sequence.

SEQ ID NO: 1

MAADGYLPDWLEDNLSEGIREWWALKPGAPQPKANQQHQDNARGLVLPG

YKYLGPGNGLDKGEPVNAADAAALEHDKAYDQQLKAGDNPYLKYNHADA

EFQERLKEDTSFGGNLGRAVFQAKKRLLEPLGLVEEAAKTAPGKKRPVE

QSPQEPDSSAGIGKSGAQPAKKRLNFGQTGDTESVPDPQPIGEPPAAPS

GVGSLTMASGGGAPVADNNEGADGVGSSSGNWHCDSQWLGDRVITTSTR

TWALPTYNNHLYKQISNSTSGGSSNDNAYFGYSTPWGYFDFNRFHCHFS

PRDWQRLINNNWGFRPKRLNFKLFNIQVKEVTDNNGVKTIANNLTSTVQ

VFTDSDYQLPYVLGSAHEGCLPPFPADVFMIPQYGYLTLNDGSQAVGRS

SFYCLEYFPSQMLRTGNNFQFSYEFENVPFHSSYAHSQSLDRLMNPLID

QYLYYLSKTINGSGQNQQTLKFSVAGPSNMAVQGRNYIPGPSYRQQRVS

TTVTQNNNSEFAWPGASSWALNGRNSLMNPGPAMASHKEGEDRFFPLSG

SLIFGKQGTGRDNVDADKVMITNEEEIKTTNPVATESYGQVATNHQSAQ

AQAQTGWVQNQGILPGMVWQDRDVYLQGPIWAKIPHTDGNFHPSPLMGG

FGMKHPPPQILIKNTPVPADPPTAFNKDKLNSFITQYSTGQVSVEIEWE

LQKENSKRWNPEIQYTSNYYKSNNVEFAVNTEGVYSEPRPIGTRYLTRN

L

[0146] In some embodiments, the n-mer can be an amino acid can be any amino acid motif as shown in Tables 1-3. In some embodiments, insertion of the n-mer in an AAV capsid can result in cell, tissue, organ, specific engineered AAV capsids. In some embodiments, the engineered capsid can have a specificity for bone tissue and/or cells, lung tissue and/or cells, liver tissues and/or cells, bladder tissue and/or cells, kidney tissue and/or cells, cardiac tissue and/or cells, skeletal muscle tissue and/or cells, smooth muscle and/or cells, neuronal tissue and/or cells, intestinal tissue and/or cells, pancreases tissue and/or cells, adrenal gland tissue and/or cells, brain tissue and/or cells, tendon tissues or cells, skin tissues and/or cells, spleen tissue and/or cells, eye tissue and/or cells, blood cells, synovial fluid cells, immune cells (including specificity for particular types of immune cells), and combinations thereof.

[0147] In some embodiments, the n-mer motif can include an “RGD” motif. An “RGD” motif refers to the presence of the amino acids RGD as the first three amino acids of the n-mer motif. Thus, in some embodiments the n-mer can have a sequence of RGD or RGDX_n, where n can be 3-15 amino acids and X, where each amino acid present can each be independently selected from the others and can be selected from the group of any amino acid. In some embodiments, the n-mer motif can be RGD (3-mer), RGDX₁ (4-mer), RGDX₁X₂ (5-mer) (SEQ ID NO: 2), RGDX₁X₂X₃ (6-mer) (SEQ ID NO: 3), RGDX₁X₂X₃X₄ (7 mer) (SEQ ID NO: 4), RGDX₁X₂X₃X₄X₅ (8 mer) (SEQ ID NO: 5), or RGDX₁X₂X₃X₄X₅X₆ (9-mer) (SEQ ID NO: 6), RGD₁X₂X₃X₄X₅X₆X₇ (10-mer) (SEQ ID NO: 7), RGD₁X₂X₃X₄X₅X₆X₇X₈ (11-mer) (SEQ ID NO: 8), RGDX₁X₂X₃X₄X₅X₆X₇X₈X₉ (12-mer) (SEQ ID NO: 9), RGDX₁X₂X₃X₄X₅X₆X₇X₈X₉X₁₀ (13-mer) (SEQ ID NO: 10), RGDX₁X₂X₃X₄X₅X₆X₇X₈X₉X₁₀X₁₁ (14-mer) (SEQ ID NO: 11), or RGDX₁X₂X₃X₄X₅X₆X₇X₈X₉X₁₀X₁₁X₁₂ (15-mer) (SEQ ID NO: 12), where X₁, X₂, X₃, X₄, X₅, X₆, X₇, X₈, X₉, X₁₀, X₁₁, X₁₂ can each be independently selected and can be any amino acid. In some embodiments, X₁ can be L, T, A, M, V, Q, or M. In some embodiments, X₂ can be T, M, S, N, L, A, or I. In some embodiments, X₃ can be T, E, N, O, S, Q, Y, A, or D. In some embodiments, X₄ can be P, Y, K, L, H, T, or S. In some embodiments, n-mers including the RGD motif can be included in a muscle-specific engineered AAV capsids. In some embodiments, the n-mer motif can be in any one of Tables 4-6. In some embodiments, the n-mer in any of Tables 4-6 can be included in a muscle specific engineered capsid.

TABLE 1
CK8 Results mRNA Second Round of Capsid Variant
Selection in C57BL6 mice-score capped at 100
					Sum of
					muscle mRNA
Variant		SEQ ID	Amino Acid	SEQ ID	score_capped
ID	Nucleotide Sequence	NO:	Sequence	NO:	at 100
1	AGGGGTGATCTTTCTACGCCT	60	RGDLSTP	1277	715.366
2	AGGGGCGACCTGAACCAATAC	61	RGDLNQY	1278	712.149
3	CGGGGTGATCTTACTACGCCT	62	RGDLTTP	1279	461.536
4	AGGGGGGATGCGACGGAGCTT	63	RGDATEL	1280	452.77
5	CGGGGTGATCAGCTTTATCAT	64	RGDQLYH	1281	444.505
6	AGAGGCGACTTATCCACACCC	65	RGDLSTP	1282	411.692
7	CGTGGTGATGTGGCGGCTAAG	66	RGDVAAK	1283	371.7
8	AGAGGAGACTTGACAACCCCA	67	RGDLTTP	1284	361.486
9	CGGGGTGATCTTAATCAGTAT	68	RGDLNQY	1285	342.712
10	CGAGGAGACACCATGAGCAAA	69	RGDTMSK	1286	325.632
11	CGCGGAGACGTAGCCGCCAAA	70	RGDVAAK	1287	315.01
12	CGGGGGGATACTATGTCTAAG	71	RGDTMSK	1288	309.567
13	CGGGGTGACGCAACAGAATTG	72	RGDATEL	1289	306.99
14	GCACGGTCAAACGACTCGGTC	73	ARSNDSV	1290	293.22
15	CGGGGTGACATGAACAACTCA	74	RGDMNNS	1291	268.677
16	ACGATGGGTGCTAATGGTACT	75	TMGANGT	1292	260.853
17	CCTAATGTTACGCAGTCTTAT	76	PNVTQSY	1293	259.718
18	CGTTTGGACCTGCAAGTCCAC	77	RLDLQVH	1294	257.65
19	GGGCTTTCTAAGGCGTCTGAT	78	GLSKASD	1295	255.938
20	GATCCTGGTCGGACGGGTACG	79	DPGRTGT	1296	253.325
21	TATCGGGGTAGGGAGGATTGG	80	YRGREDW	1297	244.83
22	AGATACGGAGAATCCATCGAA	81	RYGESIE	1298	231.696
23	AGTCTGAACAACATGGGATCG	82	SLNNMGS	1299	229.6044
24	AATAGTGATCAGCGGAATTGG	83	NSDQRNW	1300	229.031
25	CGTGGTGATATGTCTCGTGAG	84	RGDMSRE	1301	227.081
26	ATGACTGATGCGAATAGGATT	85	MTDANRI	1302	226.194
27	GTCTACAACGGCAACGTAGTA	86	VYNGNVV	1303	223.663
28	CGTGGGGATATGATTAATACG	87	RGDMINT	1304	223.46
29	AGTGGTCTTTCGCATGGTCAG	88	SGLSHGQ	1305	221.726
30	ACTGGCCAATTAGTAGGAACC	89	TGQLVGT	1306	221.181
31	GCTAATTCTATTGGGGGTCCG	90	ANSIGGP	1307	220.304
32	TACAGTCAATCGCTGTCTGAA	91	YSQSLSE	1308	220.02
33	TATCATAAGTATAGTACGGAT	92	YHKYSTD	1309	217.64
34	GCTCGTCATGATGAGCATGTG	93	ARHDEHV	1310	217
35	GCCATAGACTCTATCAAACAA	94	AIDSIKQ	1311	216.071
36	CGTTTGGACCTGCAAGTCAAC	95	RLDLQVN	1312	215
37	CGCGGCGACATGATAAACACC	96	RGDMINT	1313	214.271
38	AGTGTGTTGTCTCAGGCTAAT	97	SVLSQAN	1314	213.907
39	TTTACGGTGAATCAGGATCTT	98	FTVNQDL	1315	213.78
40	ACGGATAATGGTCTTCTTGTG	99	TDNGLLV	1316	211.787
41	TATCAGCAGACTTCTAGTACG	100	YQQTSST	1317	211.386
42	ACAGAACAATCTTACTCACGA	101	TEQSYSR	1318	210.762
43	ATTATGGGGCTTAGTCAGGCT	102	IMGLSQA	1319	208.157
44	GCTACTGCGCATCAGGATGGT	103	ATAHQDG	1320	207.212
45	TATAATGCTACTCCTTCGCAG	104	YNATPSQ	1321	206.964
46	TATACGCAGGGTATTATGAAT	105	YTQGIMN	1322	206.672
47	GAATCCCTCCCAATCTCTAAA	106	ESLPISK	1323	206.576
48	GGCACCGTCGTTCCGGGCTCC	107	GTVVPGS	1324	206.111
49	GGATTAGCTAGTCTACACCTG	108	GLASLHL	1325	204.394
50	TATATTGCTGCGGGTGAGCAG	109	YIAAGEQ	1326	204.24
51	AACACCTACCCCTTCAACGCC	110	NTYPFNA	1327	203.931
52	GTTGGTGCGAGTACGGCTTCG	111	VGASTAS	1328	202.92
53	GGATCCAACTACTTAGCAAAC	112	GSNYLAN	1329	202.857
54	GATACTGGTCGGACGGGTACG	113	DTGRTGT	1330	202.83
55	AAGCCGAATACGATGAGTGAT	114	KPNTMSD	1331	202.7282
56	GTAGACAAATCTAGCCCAGTG	115	VDKSSPV	1332	201.849
57	AGTTCGGACCCAAAAGGTCAA	116	SSDPKGQ	1333	201.825
58	TGGCAGACGAATGGTATGCAG	117	WQTNGMQ	1334	201.6943
59	ACCGGTAGCTTGAACTCTATG	118	TGSLNSM	1335	201.671
60	CATTCTAATTCGAGTCAGAAT	119	HSNSSQN	1336	200.954
61	GGCCGTGACGACCTCACAAAC	120	GRDDLTN	1337	200.911
62	GATACTTATAAGGGTAAGTGG	121	DTYKGKW	1338	200.7787
63	TATACGGCGCAGACCGGCTGG	122	YTAQTGW	1339	200
64	AATCAGGTGGGTGCGTCTGCG	123	NQVGASA	1340	200
65	ATCGACGTACTGAACGGAAGT	124	IDVLNGS	1341	200
66	TTTCGGACGGTGTATACTGGT	125	FRTVYTG	1342	200
67	GGAAACATGGTGACTCCAAAC	126	GNMVTPN	1343	200
68	GATACTTATAAGGGTAAGTGG	127	DTYNGKW	1344	200
69	ACCATCCAAGACCACATAAAA	128	TIQDHIK	1345	200
70	GGAGCAAAAGGAACCATGGGC	129	GAKGTMG	1346	200
71	ACGAGGAGCAACTCCGACGAA	130	TRSNSDE	1347	200
72	GCTACTACTCTTACTGGTGAT	131	ATTLTGD	1348	200
73	TCATACGGAGGATCTGGCCCC	132	SYGGSGP	1349	198.715
74	GAAAAATCCGTCGAATCCAAA	133	EKSVESK	1350	196.418
75	CGAGGCGACACAATGAACTAC	134	RGDTMNY	1351	195.3082
76	CGGGATCTGGGGCAGACCGGC	135	RDLGQTG	1352	194.34
77	AGTCCGCAGCTGAGTGTGATG	136	SPQLSVM	1353	194.21
78	CGAGGAGACAACAGCACACCG	137	RGDNSTP	1354	193.05
79	CCTATGGCAGGACACCCCCCG	138	PMAGHPP	1355	192.726
80	ACGGCGTATCAGGCTGGTCTG	139	TAYQAGL	1356	191.778
81	GTGGTAAACCAAGGAAACCAA	140	WNQGNQ	1357	191.737
82	GATAAGACTGAGATGCTGCAG	141	DKTEMLQ	1358	191.13
83	ACTGTGATGATGAGTACGAGG	142	TVMMSTR	1359	191.063
84	CAGCAGAATACGCGTTTGCCG	143	QQNTRLP	1360	190.1825
85	TACCAACACAACCAAGCCCAC	144	YQHNQAH	1361	189.595
86	AATCAGAGTATTAATAATATT	145	NQSINNI	1362	188.654
87	CGAGGAGACCACAGCACACCG	146	RGDHSTP	1363	187.365
88	GACTCTACACTTCACTTAAGT	147	DSTLHLS	1364	187.36
89	GCGAACATAGAAAACACGTCA	148	ANIENTS	1365	187.03
90	ACAAACGCTGCTCTAGTACCA	149	TNAALVP	1366	185.9743
91	GGGCAGAAGGAGACTACTGCG	150	GQKETTA	1367	184.457
92	GAACTTAACACCGCACACGCA	151	ELNTAHA	1368	184.059
93	GGTGTTAGTAGTAATTCTGCG	152	GVSSNSA	1369	183.964
94	AGCACAAACGCGGGACAAAGG	153	STNAGQR	1370	183.571
95	GAACAACAAAAAACAGACAAC	154	EQQKTDN	1371	182.331
96	GCTGTTGTGAATGAGAATATG	155	AWNENM	1372	182.3
97	GGCAGCGTCAGCACCAGCGCA	156	GSVSTSA	1373	181.451
98	GAGTTGGGTAGTCAGCGTATG	157	ELGSQRM	1374	181.36
99	AGAGGCGACTTATCCACACAC	158	RGDLSTH	1375	181.15
100	GACCACCAACAAGCCCTAGCT	159	DHQQALA	1376	180.295
101	AACAGATCTGACGCTCACGAA	160	NRSDAHE	1377	180.265
102	AATGTTAATGCGCAGAGTAGG	161	NVNAQSR	1378	179.918
103	ACCCAAGGGAACAACATGGTA	162	TQGNNMV	1379	179.575
104	ACGGCGCTGAATACGTATCCT	163	TALNTYP	1380	179.568
105	GTCTCTACATACCTCCTGGCA	164	VSTYLLA	1381	179.172
106	GGCGGCAACTACAACACAACT	165	GGNYNTT	1382	178.62
107	AGTAATATTAAGCCGGAGATT	166	SNIKPEI	1383	178.567
108	CCGAGGGTGCATGGTCAGGTT	167	PRVHGQV	1384	178.479
109	TCTAATTCTAATACTGCTGCT	168	SNSNTAA	1385	178.119
110	CTTGAGGTGGCGACGAGTCCG	169	LEVATSP	1386	177.75
111	CACGACGCCGACAAATTAGCT	170	HDADKLA	1387	177.05
112	GGTGTGTATATTGATGGTCGG	171	GVYIDGR	1388	176.229
113	TCGATGCAGTCGTATACGATG	172	SMQSYTM	1389	175.538
114	TCTAAAGGAAACGAACAAATG	173	SKGNEQM	1390	175.311
115	GGTCGGGATTATGCTATGAGT	174	GRDYAMS	1391	174.17
116	ACTGATGGTATTTTTCAGCCT	175	TDGIFQP	1392	174.014
117	GGGAGCCCAGTGATAGTAAAC	176	GSPVIVN	1393	173.652
118	ACATTAACAGACGTTCACCGA	177	TLTDVHR	1394	172.837
119	AAAAGCGAAGTACCCGCCCGA	178	KSEVPAR	1395	172.72
120	GTCAACACTGGCGCACTCTTG	179	VNTGALL	1396	172.648
121	AGTCAGCAGGGTTTTACTCTG	180	SQQGFTL	1397	172.124
122	AATAATAAGTCTGTGCCGGAT	181	NNKSVPD	1398	172.0753
123	AGTGTGATGGTGGGTACGAAT	182	SVMVGTN	1399	171.86
124	CGAAACGAAAACACTTACAAC	183	RNENTYN	1400	170.674
125	CAAGCTAACTTATCAATAATC	184	QANLSII	1401	170.5862
126	CCCGGACGGGACAGCAGAACG	185	PGRDSRT	1402	169.875
127	TTTCCGGCTAATGGTGGTGCT	186	FPANGGA	1403	169.639
128	GCTGGTAAGGATCTTAGTAAT	187	AGKDLSN	1404	169.592
129	GCACAATTCGAATCAGGCCGA	188	AQFESGR	1405	169.281
130	GGATACGGCAGTTACAGCAAC	189	GYGSYSN	1406	169.247
131	ACAATCGTTTCCGCTTACGCC	190	TIVSAYA	1407	168.87
132	AATGTGAGTCCTAATTTGACT	191	NVSPNLT	1408	168.739
133	AGAGGCGACTTATCAACACCC	192	RGDLSTP	1409	167.66
134	TTCTTAGAAGGAGTCGCTCAA	193	FLEGVAQ	1410	167.647
135	GGCTCCGAACGAGGAGAACGA	194	GSERGER	1411	167.585
136	TTGAATGTTGGTTCGAGTCTT	195	LNVGSSL	1412	167.104
137	CGTATTGTGGCTAATGAGCAG	196	RIVANEQ	1413	166.96
138	CAATCTATCGGCCACCCCGTT	197	QSIGHPV	1414	166.7759
139	GGTGGTATGTCGGCGCATTCG	198	GGMSAHS	1415	166.775
140	CATTCTACGACGTCTATGACG	199	HSTTSMT	1416	166.711
141	ACTGTAAACGGTACGAACGTA	200	TVNGTNV	1417	166.64
142	CTTGCGCCTGATAATATTGGG	201	LAPDNIG	1418	166.005
143	CAAACAGCGACTCTCGTGGCA	202	QTATLVA	1419	165.921
144	GCATCAGCACCGTCTGAATTC	203	ASAPSEF	1420	165.64
145	TCGATGGAGGGTCAGCAGCAT	204	SMEGQQH	1421	165.62
146	CAAGACGTAGGACGCACGAAC	205	QDVGRTN	1422	164.147
147	GTCTACAACGGCAACGAAGTA	206	VYNGNEV	1423	164.11
148	GCACAGGCGCAGACAGGCTGG	207	AQAQTGW	1424	163.93
149	CGGCTGGATCTGACGCATACG	208	RLDLTHT	1425	163.75
150	GCTGCACACGGCCGCGAACAA	209	AAHGREQ	1426	163.577
151	AGAGGCGACTTATACACACCC	210	RGDLYTP	1427	163.43
152	GGTATGCAGCAGAGGGAGAAG	211	GMQQREK	1428	163.075
153	CAGACTCAGGCGAGTACTAAT	212	QTQASTN	1429	161.336
154	CGGGACACCAACGCCCTCGGA	213	RDTNALG	1430	161.225
155	TCGAGTCAGATTTCTAATAGT	214	SSQISNS	1431	161.063
156	CAGTCGGTTAATAGTACGAGT	215	QSVNSTS	1432	160.873
157	GCTCTGGAGAGGGCTCAGTAT	216	ALERAQY	1433	160.837
158	CATACTGGGCATAGTTCTGTG	217	HTGHSSV	1434	160.068
159	CGGGGAGACATGACCCGAGCA	218	RGDMTRA	1435	159.605
160	TTTCAGCGTGATCTTGGGCAT	219	FQRDLGH	1436	159.442
161	ACAACCGGCGACATAATACGC	220	TTGDIIR	1437	159.11
162	TCTTTTCAGACGGATCGTGCG	221	SFQTDRA	1438	159.04
163	CAATCCAGCGACGGCCGAGTG	222	QSSDGRV	1439	158.634
164	ACTTCTGGGGCTTTGACCCGG	223	TSGALTR	1440	158.32
165	AATTCGAATACTGTGAATACG	224	NSNTVNT	1441	157.71
166	ATCTCCGGTAGTAGCAGTCTA	225	ISGSSSL	1442	157.64
167	AACGACAAATCAACCAACGTA	226	NDKSTNV	1443	157.594
168	ATCGTACTTGCTCCCACATCG	227	IVLAPTS	1444	157.48
169	TCAGGCGTCAACTACGGTGTC	228	SGVNYGV	1445	157.321
170	GTCGGCGCCCAACGGGACCCC	229	VGAQRDP	1446	157.055
171	ACGGGTATGAATAGTAATAAG	230	TGMNSNK	1447	156.85
172	ATCGAAGCCTACTCACGAGAC	231	IEAYSRD	1448	156.774
173	TTACACACAACACTAATGCCC	232	LHTTLMP	1449	156.364
174	TCTGATAATCATCTGAAGACT	233	SDNHLKT	1450	156.334
175	CGAAACGAAGACAAAGGAGGA	234	RNEDKGG	1451	156.027
176	ACGAAGGGTGCTAATGGTACT	235	TKGANGT	1452	155.56
177	GTCTACAACGGCAACGTAGAA	236	VYNGNVE	1453	155.56
178	TCAAACAGCGGAGGCAACCAC	237	SNSGGNH	1454	155.294
179	GTAGCCGCGGGACCAGAAGCG	238	VAAGPEA	1455	154.25
180	ACGTCTCTTAGTGGTAGTGCG	239	TSLSGSA	1456	153.988
181	GTTGGGCTGCAGAGTAATACT	240	VGLQSNT	1457	153.453
182	CACACCGCCCACAGCGTGGAC	241	HTAHSVD	1458	153.3866
183	AACGTGGGAATGAGCTCAACC	242	NVGMSST	1459	153.212
184	CATGCGGATGTGAATGCTGGG	243	HADVNAG	1460	153.21
185	AAAGCGGGACAACTAGTGGAA	244	KAGQLVE	1461	153.178
186	AGTACTTTTAGTGTGCTGCCT	245	STFSVLP	1462	153.09
187	CCTCAGTCTCCGAGTCGGGTT	246	PQSPSRV	1463	152.823
188	CACACCGCCACCCTTAGCAGC	247	HTATLSS	1464	152.8
189	CTTCCGCGTCATGATCAGTAT	248	LPRHDQY	1465	152.412
190	CAAGTGAACAACCCACTCACA	249	QVNNPLT	1466	151.574
191	ACAACAGAAACCGCACGAGGT	250	TTETARG	1467	151.4255
192	GTTCATGGGACGTTGACTTAT	251	VHGTLTY	1468	150.654
193	TATAGTACTGATCTTAGGATG	252	YSTDLRM	1469	150.626
194	GCACACGCTACCTCAAGCACT	253	AHATSST	1470	150.587
195	AGGGAGAGTGCTGCTCTGGCG	254	RESAALA	1471	150.506
196	AAGGATACTAATCAGCAGATT	255	KDTNQQI	1472	150.189
197	AGTATGCAATCATACACCATG	256	SMQSYTM	1473	148.994
198	ACAGCCTACTCGCCCACAGTC	257	TAYSPTV	1474	148.946
199	GAATCTGCCCACCAAAGAATA	258	ESAHQRI	1475	148.867
200	AGATACACAACAGCACAACAA	259	RYTTAQQ	1476	148.802
201	ACGTCTGTGGCGAATGTGAGT	260	TSVANVS	1477	148.731
202	AGGGATCAGCATACTTCTATT	261	RDQHTSI	1478	148.687
203	TCTGTTACGTCTTCTGGTCCG	262	SVTSSGP	1479	148.574
204	GCGGTTGTTCTGAATAGTAAT	263	AVVLNSN	1480	148.476
205	CCTGGGAATCCGTCTAGTAAT	264	PGNPSSN	1481	147.792
206	ACGGGGTCTACTACTCAGCTT	265	TGSTTQL	1482	147.767
207	GCTAATGAGCATAATGTGGGT	266	ANEHNVG	1483	147.569
208	ATGCAAAGAGAAGCAGCCAAC	267	MQREAAN	1484	147.562
209	TTAACCGACACAAACACCCGG	268	LTDTNTR	1485	147.306
210	CGAATGACCGAAATATCATAC	269	RMTEISY	1486	146.933
211	AAAGTGGACATGACCTCCAAA	270	KVDMTSK	1487	146.392
212	AGAGGAGACTTATCCACACCC	271	RGDLSTP	1488	146.3
213	CAAGCAAAAGCTAGCACAACT	272	QAKASTT	1489	146.214
214	CTACCCTCAACAGAAACTTTG	273	LPSTETL	1490	145.892
215	AGTAGTGCGCTTAATGCGTAT	274	SSALNAY	1491	145.667
216	TCGTCTGATCCTAAGGGGCAG	275	SSDPKGQ	1492	145.644
217	TTAGACGTGACGAGAATGAGA	276	LDVTRMR	1493	145.51
218	GCGGATGGTGGTGATAAGGGG	277	ADGGDKG	1494	145.45
219	ATGCTGTCTCAGGTTACGTTG	278	MLSQVTL	1495	145.32
220	AGTGTTAGTTCTGTGGTGTTG	279	SVSSWL	1496	145.202
221	ACCGAATCGCAAACCATGAGG	280	TESQTMR	1497	145.0149
222	TTCGGATCCCAAGAAAAACTC	281	FGSQEKL	1498	144.467
223	ACAGCCGGCGGCGAACGCGCC	282	TAGGERA	1499	144.445
224	GATCATAGTAAGCAGAGTTCG	283	DHSKQSS	1500	144.0179
225	ATTGATAGTACTTGGAATACG	284	IDSTWNT	1501	143.92
226	TCGCCTCGCCCCGAACTCCGA	285	SPRPELR	1502	143.362
227	AGTATTGCGACTGCTACTAGT	286	SIATATS	1503	143.312
228	GTAATAGGCGGACACGGGACT	287	VIGGHGT	1504	143.136
229	AGCACCGCCATGTACCCCCAC	288	STAMYPH	1505	142.798
230	CGGGACTTGAGACCCGTGACG	289	RDLRPVT	1506	142.461
231	GCTCATCTGACTGATCTTCCG	290	AHLTDLP	1507	142.37
232	TTTCTGAATAGTACGCAGCTT	291	FLNSTQL	1508	142.276
233	TTAAACAACAGTGCCACAGTC	292	LNNSATV	1509	142.021
234	GATCGTCCGAATAATATGACG	293	DRPNNMT	1510	141.945
235	TCATCGTCAGACTCACCCAGA	294	SSSDSPR	1511	141.849
236	CGCTTGGACGTTGGAAGCCCG	295	RLDVGSP	1512	141.82
237	GCGCAGCAGAGTCTTCATGGT	296	AQQSLHG	1513	141.401
238	ATGGGGAAGCATGAGGGTCTT	297	MGKHEGL	1514	141.2916
239	GAGAATGCTCGTGAGGGTGTG	298	ENAREGV	1515	140.87
240	ACCGTATCTCTCTCGGAAGGC	299	TVSLSEG	1516	140.529
241	CTTAACACACTAATCGACCGG	300	LNTLIDR	1517	140.256
242	GAACTCTCCGTTCCGAAACCA	301	ELSVPKP	1518	140.203
243	AAAGACAAAAACGTATACATA	302	KDKNVYI	1519	140.171
244	AATGCGAATGGGCCTGTGAGT	303	NANGPVS	1520	140.158
245	CTTACTACGAATGGTATGCTG	304	LTTNGML	1521	140.147
246	GCCGGCGAATCTTCACCCACA	305	AGESSPT	1522	139.95
247	AGTGGGATTGGTACTTATTCT	306	SGIGTYS	1523	139.76
248	GTCAGATCTATGGACGAATTG	307	VRSMDEL	1524	139.74
249	ATGAACACCGGCTCTTCGAGT	308	MNTGSSS	1525	139.328
250	GGGGTGACTGTTAGGGAGCTT	309	GVTVREL	1526	139.099
251	CAGATTTTGAATTATAGTGTG	310	QILNYSV	1527	138.991
252	ATGGCGGGTGAGTATAGGGTT	311	MAGEYRV	1528	138.933
253	TGGTCGCATGATCGGCCTACT	312	WSHDRPT	1529	138.703
254	TGCAAAAACAACTCAGAATGC	313	CKNNSEC	1530	138.668
255	TTGACGACGAATAGTCATTAT	314	LTTNSHY	1531	138.525
256	ATGCTTGTTCAGAATACTCCT	315	MLVQNTP	1532	138.3
257	CGTGGTGCGACTGAGCATGCG	316	RGATEHA	1533	138.186
258	GCTTCGAATGGGAGTATGGGT	317	ASNGSMG	1534	138.1181
259	AATAGTTATACTGCTGGGAAG	318	NSYTAGK	1535	137.4033
260	TCCACCCAAGGAGCCATCCTC	319	STQGAIL	1536	137.294
261	TGGAATACGAATATGGCGATT	320	WNTNMAI	1537	137.17
262	GTCTCATCGTACGAAAAAATA	321	VSSYEKI	1538	137.055
263	GTGCTGAGTACGGGGCAGCGG	322	VLSTGQR	1539	136.9001
264	CCTATACCCCACGGTTCATCC	323	PIPHGSS	1540	136.523
265	AACGTGTCACTAACGCAAACG	324	NVSLTQT	1541	136.4003
266	TCTACCATCGGCAACAGCACG	325	STIGNST	1542	136.393
267	TCTGAGAAGCTGACTGATAAG	326	SEKLTDK	1543	136.36
268	TCCAAAGACTCGAACATAAGT	327	SKDSNIS	1544	136.166
269	GCGAATAGTAATCATGAGCGT	328	ANSNHER	1545	136.102
270	AGGGATACGGGTGATAAGGCT	329	RDTGDKA	1546	135.913
271	AGAACAGACACGCCGTCAACC	330	RTDTPST	1547	135.583
272	CCTACTATGTCGAGTCTGAAT	331	PTMSSLN	1548	135.539
273	GATATTACTAATCAGTCGTAT	332	DITNQSY	1549	135.473
274	CTTGTAAAACCGGAAACTTGG	333	LVKPETW	1550	134.988
275	GGGACTTCCTTGGAAAACCGA	334	GTSLENR	1551	134.981
276	GCTGCTGGTAATCCTACTCGT	335	AAGNPTR	1552	134.779
277	CACAACGTCGGCCTAGGACAC	336	HNVGLGH	1553	134.677
278	GTATCAACGACAACGGACCGG	337	VSTTTDR	1554	134.639
279	TATTTGTCGTCTGGTAAGATG	338	YLSSGKM	1555	134.553
280	GATAGTCGGAATGCTGCTTTG	339	DSRNAAL	1556	134.213
281	GTGGAGCGGAATACTGATATG	340	VERNTDM	1557	133.962
282	ACTGTTGGGAGTAATTCTATT	341	TVGSNSI	1558	133.95
283	GTGCGGTCTGGTAATAAGCCG	342	VRSGNKP	1559	133.87
284	GGCAGTTCGGGGAACAGCGGA	343	GSSGNSG	1560	133.776
285	TCTACTTCAATAGGAGTGGTA	344	STSIGW	1561	133.69
286	CCGAGTCAGAGTAGGTCGCTT	345	PSQSRSL	1562	133.6751
287	CGGAATGAGAATCTTAATAAT	346	RNENLNN	1563	133.26
288	TCGTTGGGTAAGAGGGAGGAG	347	SLGKREE	1564	133.032
289	TCACGCTTGGACTCGAGCTCC	348	SRLDSSS	1565	132.783
290	GATTCGACGTATGTTTTGGCT	349	DSTYVLA	1566	132.54
291	GAGCGTAATCCTATTTCTGAT	350	ERNPISD	1567	132.49
292	GTTAGCTCCGGCCACACGAAA	351	VSSGHTK	1568	132.466
293	AAGTATACGGAGTCGAATGCG	352	KYTESNA	1569	132.305
294	AACCGCAACTCAGTTGGGACT	353	NRNSVGT	1570	132.2576
295	CACGAAAGCCACTACGTGTCA	354	HESHYVS	1571	132.014
296	ACGACTGGGGGGACGGGGATG	355	TTGGTGM	1572	131.954
297	GCGACTGATAAGATGACTCCT	356	ATDKMTP	1573	131.931
298	TCCGCGTCTAGCGGCGCTACA	357	SASSGAT	1574	131.886
299	TCAACCACTACTGGCCACATG	358	STTTGHM	1575	131.581
300	ATAATAGCATCCTCTACCACG	359	IIASSTT	1576	131.506
301	GATACTGGGTCTAGGATTGCG	360	DTGSRIA	1577	131.486
302	TGGGCTGATGATTCGCAGCGG	361	WADDSQR	1578	131.47
303	AGGGGTAACACTCTCGAAATG	362	RGNTLEM	1579	131.381
304	AATCTGCAGGTGAATGCGAAT	363	NLQVNAN	1580	131.172
305	GCGACGACTCAGCTGATGACT	364	ATTQLMT	1581	130.96
306	GCTGATACGAATATTATTGTG	365	ADTNUV	1582	130.47
307	GCCATAACAATCACTCAAAAA	366	AITITQK	1583	130.225
308	GACTCCAACAAAGGAGCGACG	367	DSNKGAT	1584	130.1749
309	GGCAACGCTTCCGGAAACCCA	368	GNASGNP	1585	129.97
310	ACGATGGGTGCTAAAGGTACT	369	TMGAKGT	1586	129.92
311	TATCTGCAGACGGGTACTCTG	370	YLQTGTL	1587	129.907
312	GCATTACACACCAAAGACCTA	371	ALHTKDL	1588	129.846
313	GTCGACAAAAGCGAAGCCGTC	372	VDKSEAV	1589	129.734
314	GGGAGGACGGATCTTATGGCG	373	GRTDLMA	1590	129.651
315	GGCACGGAACCGCGCACTGCA	374	GTEPRTA	1591	129.37
316	AGAGGCGACATGTCACGAGAA	375	RGDMSRE	1592	129.137
317	CGGGGGGATACTAAGTCTAAG	376	RGDTKSK	1593	128.94
318	GGGACATTAGCCTCAATGTCC	377	GTLASMS	1594	128.734
319	CAGAAGTCTGTGACGTATTCG	378	QKSVTYS	1595	128.602
320	AGTACGGGGCAGACTCTTGTT	379	STGQTLV	1596	128.1669
321	TCGCACATAAACATGGGGTCG	380	SHINMGS	1597	128.101
322	GCGTTGAATGGTACTGGTAAT	381	ALNGTGN	1598	128.045
323	ACTACGAGTTCGAATCAGCAT	382	TTSSNQH	1599	128.003
324	AAAAACTACGCAAGCACCGAC	383	KNYASTD	1600	127.84
325	GAATCCACAAGCAGGACGTAC	384	ESTSRTY	1601	127.765
326	CCGCGTTCTATTACGGAGTTG	385	PRSITEL	1602	127.623
327	TACATAGCCGGAGGAGAAAAA	386	YIAGGEK	1603	127.544
328	ACTAGTAATTATATGCATGAG	387	TSNYMHE	1604	127.522
329	TTGGATCCTAATAGTACTCGG	388	LDPNSTR	1605	127.175
330	CACAGTGACATGGGCTCAAGC	389	HSDMGSS	1606	127.01
331	GACACCGCCAACCGATCCACA	390	DTANRST	1607	127.01
332	AACGCCGGACACAGCGGTCAA	391	NAGHSGQ	1608	126.611
333	AGTTTGGGGTCGGATCGTATG	392	SLGSDRM	1609	126.579
334	GACAACCAACAAGCCCTAGCT	393	DNQQALA	1610	126.49
335	CCATCCTCAGCGGGTAGCACA	394	PSSAGST	1611	126.201
336	GACAGGAAAGGGTACGACGCA	395	DRKGYDA	1612	126.06
337	GGAGGAAACCAAAACCTTACT	396	GGNQNLT	1613	125.7806
338	GTGAATCTGAATGAGACGGAG	397	VNLNETE	1614	125.719
339	TCCCCCGGCAACGGGTTGCTA	398	SPGNGLL	1615	125.687
340	TCTGTCGGGGACCTCACAAAA	399	SVGDLTK	1616	125.627
341	CGATACGAATCCGTCGGACTC	400	RYESVGL	1617	125.54
342	ACGAGAGAATTGACAAAAAAC	401	TRELTKN	1618	125.47
343	ACTCCAACTAACGGGAACCCT	402	TPTNGNP	1619	125.37
344	GCGACTGATCAGCGTTCGAGG	403	ATDQRSR	1620	125.26
345	GGAACATCGGCAGAATCACGC	404	GTSAESR	1621	125.214
346	AGGATGCTCTCTACTTTGCCT	405	RMLSTLP	1622	125.088
347	GGTATCAACTCCTCACACTTC	406	GINSSHF	1623	125.044
348	AGTAGCTCAACTGAAGGGCAA	407	SSSTEGQ	1624	124.971
349	GACAAACAACAAACCGGACAA	408	DKQQTGQ	1625	124.923
350	ACCCAACACCTACCATCCACA	409	TQHLPST	1626	124.773
351	GGTCTGGGGCAGCCTCAGTTG	410	GLGQPQL	1627	124.752
352	GTGACTAATGAGAGTCGTGCT	411	VTNESRA	1628	124.728
353	GGCAACTCGAACTACCGAGAA	412	GNSNYRE	1629	124.482
354	TGGAATGCTGAGAATAGTAAG	413	WNAENSK	1630	124.373
355	CCTGGGAGTCAGCGTCAGGAT	414	PGSQRQD	1631	124.325
356	CATACGTATTCGCAGGCTGAT	415	HTYSQAD	1632	124.3
357	ACTGCCGGCAACCTAAGAAGT	416	TAGNLRS	1633	124.203
358	GGCAGACACCTTCAATCGGAC	417	GRHLQSD	1634	124.19
359	AACAACGCACACACCGCCACT	418	NNAHTAT	1635	124.118
360	AGTACGAGTCAGGAGAATAGG	419	STSQENR	1636	124.0658
361	AGGGGTGATACTATGAATTAT	420	RGDTMNY	1637	124.04
362	CCGGTTGCTACTCAGCATGCG	421	PVATQHA	1638	123.9189
363	GGGCATTTGAATGCTCCGACT	422	GHLNAPT	1639	123.495
364	CAAATATTAAACTACTCAGTC	423	QILNYSV	1640	123.4
365	CAAAACCACGCGTCTGGTGAA	424	QNHASGE	1641	123.372
366	GGTTTAACAGGGCGGGAACTA	425	GLTGREL	1642	123.32
367	GACGTAGCCGTGACTCAACAC	426	DVAVTQH	1643	123.31
368	GCAACTTACACCGGGCGAACA	427	ATYTGRT	1644	123.292
369	AAAGAACTACAATGGCAACGA	428	KELQWQR	1645	123.251
370	GCTAGTTATAGTAGTATGGTG	429	ASYSSMV	1646	123.193
371	GTTATTAGTCATGGGGCGCTG	430	VISHGAL	1647	123.094
372	CCTATACACCACGGTTCATCC	431	PIHHGSS	1648	123.09
373	GTGGATAAGAATCATCCTTTG	432	VDKNHPL	1649	123.04
374	ACCTCGGGTGACCGGTACACG	433	TSGDRYT	1650	122.844
375	GGGACAAAAAGCTGGCCTGTC	434	GTKSWPV	1651	122.8432
376	TACAACGCCCACGAATCATTC	435	YNAHESF	1652	122.813
377	AGAGTCCACGACACTCCTTCA	436	RVHDTPS	1653	122.7503
378	GCACAAATCGAATCAGGCCGA	437	AQIESGR	1654	122.66
379	TGGAAGGATAATATGCGGATG	438	WKDNMRM	1655	122.624
380	ATGCCTAGTGAACCACCAGGG	439	MPSEPPG	1656	122.51
381	CGTGGTGATTATCCGACGTCG	440	RGDYPTS	1657	122.487
382	TTTCATAATGAGTCTTATGGG	441	FHNESYG	1658	122.36
383	TTGAATACGATGATTGATAAG	442	LNTMIDK	1659	122.272
384	TCCACACTAAGCCAAGGAGCA	443	STLSQGA	1660	122.2662
385	CCTTTGCACAACATACCTCCT	444	PLHNIPP	1661	122.24
386	GCTTCGTCTACGTTTTTGCCT	445	ASSTFLP	1662	122.24
387	ATGGAAGGAATGGGACTCGGA	446	MEGMGLG	1663	122.04
388	AAGGATTATAAGCCGTATGCT	447	KDYKPYA	1664	121.95
389	AATTTGCAGTCTGGTGTTCAG	448	NLQSGVQ	1665	121.91
390	ACAACTCTTAGCCAACAAAGC	449	TTLSQQS	1666	121.82
391	CTTATGTCGTCTACTTCCTCA	450	LMSSTSS	1667	121.536
392	ACTGGCCAAGGATTCTCGGCA	451	TGQGFSA	1668	121.45
393	TCTACAATCGGCAACAGCACG	452	STIGNST	1669	121.27
394	CTGAGGGCGAGTGAGGCTCCG	453	LRASEAP	1670	121.2297
395	CAGCCTAATAATGGTAATCAT	454	QPNNGNH	1671	121.02
396	TCGTCAGACGTTACCAGACAA	455	SSDVTRQ	1672	120.98
397	CGGGGTGACGCAACAGAAATG	456	RGDATEM	1673	120.74
398	TATAGGGGTAGGGAGGATTGG	457	YRGREDW	1674	120.58
399	AGCTTGCAACAATCACAATTG	458	SLQQSQL	1675	120.491
400	AAGCCGACTGCGAATGATTGG	459	KPTANDW	1676	120.3784
401	CGTCTGACTGATACTATGCAT	460	RLTDTMH	1677	120.35
402	CTTCATGGGAATTATAGTCCG	461	LHGNYSP	1678	120.346
403	ATTCCGGTTGGGGCGATGGCT	462	IPVGAMA	1679	120.248
404	CCGAACACCGCCTCAAACTTC	463	PNTASNF	1680	120.24
405	ACGAGTAGAGAAGTCAAAGGG	464	TSREVKG	1681	120.171
406	GACACGTCCTCCGGCAACAGG	465	DTSSGNR	1682	119.94
407	GAAGCAGTAACAAGTAAATGG	466	EAVTSKW	1683	119.919
408	CTAATCACAGCCACCACTAAC	467	LITATTN	1684	119.872
409	GATGGGGGTCGTTCGGGTATT	468	DGGRSGI	1685	119.847
410	TTCATGGAAGTCATGAAAAAC	469	FMEVMKN	1686	119.82
411	TCCTACCAAAACCCACCACCA	470	SYQNPPP	1687	119.701
412	ACTAATGTGACGTTTAAGCTT	471	TNVTFKL	1688	119.681
413	ATTTCTACGCATACGATGACG	472	ISTHTMT	1689	119.64
414	GAAACCCAAGGAGCAAGATAC	473	ETQGARY	1690	119.591
415	GCGGCTTATGAGCATGCGCCT	474	AAYEHAP	1691	119.588
416	TCAACGAACGACCGTGCGTTA	475	STNDRAL	1692	119.57
417	TTCACCGAACGCGCACTCCAA	476	FTERALQ	1693	119.423
418	GTAGCGGGCTTAGTCGACATA	477	VAGLVDI	1694	119.41
419	AGCTCGGTAACTAACCTTGCA	478	SSVTNLA	1695	119.38
420	GATACTACTACTGGTCATCTT	479	DTTTGHL	1696	119.27
421	ACGCGTAATTTGTCTGAGAGT	480	TRNLSES	1697	118.919
422	CAGGTGAATGTTGGGCCTGGT	481	QVNVGPG	1698	118.831
423	AAACAAACGATGTCCGACACA	482	KQTMSDT	1699	118.829
424	ATGTCGACAACCAGCAAAACT	483	MSTTSKT	1700	118.7215
425	ACTACAATAGGGACAAACCAA	484	TTIGTNQ	1701	118.676
426	GGGACTCTGACGCCGAATCTT	485	GTLTPNL	1702	118.622
427	TTTGATAGTTATAATATTGTG	486	FDSYNIV	1703	118.51
428	CGTGGTGCGCCTGAGCAAGCG	487	RGAPEQA	1704	118.47
429	ATCGAAAACGTAAACCACTTG	488	IENVNHL	1705	118.42
430	AGGTCTCTGGAGAGTCAGGCT	489	RSLESQA	1706	118.231
431	CAGTATACGAGTCTGAGTCCG	490	QYTSLSP	1707	118.006
432	ACGAAGGGTTATAATGATCTT	491	TKGYNDL	1708	117.876
433	GTCGCCTCGATGGTACACAAC	492	VASMVHN	1709	117.874
434	TCCACAACCCACACCTCAGCA	493	STTHTSA	1710	117.821
435	CTTGCGCACCCACAACCAAAC	494	LAHPQPN	1711	117.542
436	TCGATAAACAACATAGGCGCA	495	SINNIGA	1712	117.538
437	GCTATAGACTCCATCAAAATG	496	AIDSIKM	1713	117.472
438	TCTATGTATGGGCAGGCTGGG	497	SMYGQAG	1714	117.362
439	GAGTATGCTAATGCTAAGACT	498	EYANAKT	1715	117.351
440	TATCGGGCTTCGGATGTGGCG	499	YRASDVA	1716	117.348
441	GTTAGTTTGGAGAGTCGGTTG	500	VSLESRL	1717	117.332
442	ATTGAGACTAGTTCGCGTTCG	501	IETSSRS	1718	117.176
443	ATGGGAGTGAAACCCGAACAA	502	MGVKPEQ	1719	116.975
444	GCGCTTCCGTCTCGTGAGCGG	503	ALPSRER	1720	116.914
445	GGCACCGGATCTTCAGCGCAC	504	GTGSSAH	1721	116.896
446	CAAACGAACACCAACGACAGA	505	QTNTNDR	1722	116.664
447	GTATTACACTCTGTATCAGCA	506	VLHSVSA	1723	116.583
448	CCTTATTCTGCTACTGATCGG	507	PYSATDR	1724	116.577
449	GCAAACTCCGGATTACACAAC	508	ANSGLHN	1725	116.505
450	TATGAGAGTACTCATGTTAAT	509	YESTHVN	1726	116.418
451	AACAACGCACTAGTAGGAAGT	510	NNALVGS	1727	116.34
452	GGTATCAACTCCTCACACATC	511	GINSSHI	1728	116.28
453	AGTATTTCTGATAAGAATCAG	512	SISDKNQ	1729	116.141
454	GACCACCAACAAGCCCTAGCA	513	DHQQALA	1730	116.13
455	GACTCTACCAAAGCCATGCAA	514	DSTKAMQ	1731	116.116
456	ACTATTACTAGTCAGTCGGTG	515	TITSQSV	1732	115.95
457	GGCGCCCGTACAATCTTAGAC	516	GARTILD	1733	115.938
458	GAGCATAGTCCTACGACTGGT	517	EHSPTTG	1734	115.8995
459	GGGCTCACAGGATACCCAATG	518	GLTGYPM	1735	115.844
460	ACGATGGAATCCGGCCGCCAC	519	TMESGRH	1736	115.82
461	TCTGCGTCGAAAGTGGAATAC	520	SASKVEY	1737	115.719
462	GATAAGTCTAATTATAGTATT	521	DKSNYSI	1738	115.714
463	TTCAACGAAACTGCCGGGCGA	522	FNETAGR	1739	115.65
464	CAAAAATCGGAAACCTACACT	523	QKSETYT	1740	115.528
465	GCACTTACCCGTATGCCTAAC	524	ALTRMPN	1741	115.476
466	CGTAACGGCTCCGCCCAAAGC	525	RNGSAQS	1742	115.465
467	GCGAGGGATACGCCTGGGATT	526	ARDTPGI	1743	115.432
468	ATTGTTAATGCTGAGATTTAT	527	FVNAEIY	1744	115.31
469	CGACAAGGCGACTTAAAAGAA	528	RQGDLKE	1745	115.3059
470	CGAAACAACCCATCGCACGAC	529	RNNPSHD	1746	115.224
471	CTCGCCCACAACTACTTAAGC	530	LAHNYLS	1747	115.195
472	AACACCCACAACCTACAAATG	531	NTHNLQM	1748	115.171
473	CGAGGAGACCACAGCACACAG	532	RGDHSTQ	1749	115.12
474	CTCCACGGAGTCAGCAGTATA	533	LHGVSSI	1750	115.105
475	GGTATTAATCATGTGGCGTCT	534	GINHVAS	1751	115.102
476	ACTGATAAGCTTCAGGGTGTG	535	TDKLQGV	1752	115.062
477	GGAACCTCCATAGACTACGTA	536	GTSIDYV	1753	115.053
478	TCGAACACTGCCCCCCCCCCC	537	SNTAPPP	1754	115.034
479	ACTGCTAAGAGTTATGGGCCT	538	TAKSYGP	1755	115.006
480	GACCACCAACAAGCACTAGCT	539	DHQQALA	1756	114.98
481	ACACAAGTAGTCGCAAGAACA	540	TQWART	1757	114.9299
482	AGTCCTCCTAGTACGTCGGGT	541	SPPSTSG	1758	114.816
483	CCTATGCGAACACCACCGTAC	542	PMRTPPY	1759	114.806
484	GCTGCTGGTAATACTACTCGT	543	AAGNTTR	1760	114.78
485	AGAGGCGACTAATCCACACCC	544	RGD*STP	1761	114.78
486	CTAGCGAAAACTGTCGCTATC	545	LAKTVAI	1762	114.722
487	TCTAAATCTGAAAACCTGCAA	546	SKSENLQ	1763	114.59
488	ACTCAGACGTCGTATGCTACG	547	TQTSYAT	1764	114.505
489	ACTGGGGATAGGACTTCGGTG	548	TGDRTSV	1765	114.4766
490	ATATCGCAAGGCTCGAGCCTC	549	ISQGSSL	1766	114.305
491	CTTGTTCAGATGGGGAGTGTG	550	LVQMGSV	1767	114.256
492	TTATCCGCAACATCTACGATG	551	LSATSTM	1768	114.245
493	CAAAACCACAACGAACTAAAA	552	QNHNELK	1769	114.217
494	CGTGGTGCGCCTGAGCATGCG	553	RGAPEHA	1770	114.09
495	TCTTCTTTCGGAAAAGACAAC	554	SSFGKDN	1771	113.982
496	AACGCTAACGCCGGTGGAAAC	555	NANAGGN	1772	113.958
497	GATCATCATCCTCAGAGTCGT	556	DHHPQSR	1773	113.83
498	ATGAGGCATGAGGCTCCTCTT	557	MRHEAPL	1774	113.819
499	AAGGGGGATGGTGCTTATGAG	558	KGDGAYE	1775	113.742
500	CCTATGAATGGTATTCTGTTG	559	PMNGILL	1776	113.722
501	AGTAGTGGGGGTATGAAGGCG	560	SSGGMKA	1777	113.69
502	GTGCTGGTTACTCAGAATCAT	561	VLVTQNH	1778	113.631
503	GAGATTAATAATCGGACTGGT	562	EINNRTG	1779	113.588
504	TTACCAACAGGCGTCCTGCCC	563	LPTGVLP	1780	113.561
505	GCCTACGGTATCAGAGAAGTG	564	AYGIREV	1781	113.547
506	TCGACAAACTCTATAGGCGCC	565	STNSIGA	1782	113.471
507	GTGCAGTTGACGCATAATGGG	566	VQLTHNG	1783	113.43
508	GTTCAGTTGGAGAATGCGAAT	567	VQLENAN	1784	113.43
509	GGAAAAGCCAACGACGGTTCT	568	GKANDGS	1785	113.427
510	ACCGGGGTTCGAGAAACCATA	569	TGVRETI	1786	113.41
511	GGCCTGAACCAGATCACATCG	570	GLNQITS	1787	113.4
512	ACGGAGAAGGCGAGTCCTCTG	571	TEKASPL	1788	113.381
513	TTTCTGGAGGGTGTTGCGCAG	572	FLEGVAQ	1789	113.333
514	ACGAATTATAATATTGGTCCG	573	TNYNIGP	1790	113.318
515	AGAGGAGACTTGACAACCACA	574	RGDLTTT	1791	113.29
516	ATGATGAATGTGAGTGGTCAT	575	MMNVSGH	1792	113.09
517	TCTCAGTCGATTAATGGGCTT	576	SQSINGL	1793	113.084
518	CTCACGACTTTAACTAACCAC	577	LTTLTNH	1794	113.033
519	AACTCTGTTCAATCCACCCCA	578	NSVQSTP	1795	113.021
520	TATAATACGGATCGGACTAAT	579	YNTDRTN	1796	113.001
521	GAGAAGCCTCAGCATAATAGT	580	EKPQHNS	1797	112.98
522	ACGATGGCTACAAACTTAAGT	581	TMATNLS	1798	112.937
523	GTGGGGACGCATTTGCATTCG	582	VGTHLHS	1799	112.918
524	GACGCCCACCACTCAAGCAGC	583	DAHHSSS	1800	112.88
525	CTTGTGGGGACTTTGGTGTAT	584	LVGTLVY	1801	112.853
526	TATGGTGTGCAGGCGAATAGT	585	YGVQANS	1802	112.806
527	GTTTTGTCTGATAAGGCGTAT	586	VLSDKAY	1803	112.787
528	CTTGAGGGTCAGAATAAGACG	587	LEGQNKT	1804	112.731
529	GAGGTTAGTAATAATAATTAT	588	EVSNNNY	1805	112.69
530	GCCCACCAACAAGCCCTAGCT	589	AHQQALA	1806	112.67
531	CTTCCGACCACACTCAACCAC	590	LPTTLNH	1807	112.667
532	TACATAGCAGGTGGTGAACAA	591	YIAGGEQ	1808	112.6513
533	AATTCTGGTACTCTTTATCAG	592	NSGTLYQ	1809	112.609
534	CGGGGTCTGCCTGATGTTAAT	593	RGLPDVN	1810	112.43
535	AACCAACAACTATCCCACTCA	594	NQQLSHS	1811	112.375
536	AATCCTAGTTATGATCATCGG	595	NPSYDHR	1812	112.363
537	ATAGACAGCGACACCTTCGTA	596	IDSDTFV	1813	112.355
538	ACCGCTTACCTTGCGGGATTA	597	TAYLAGL	1814	112.17
539	CATAGTAATGTTAGTCTTGAG	598	HSNVSLE	1815	112.162
540	GGTAATAATTTGAGTTTGTCT	599	GNNLSLS	1816	112.16
541	GTTATGGATACGCATGGGATG	600	VMDTHGM	1817	112.145
543	ACTAACGCCATCTCTCAAACG	602	TNAISQT	1819	112.063
544	GCAACACACGCCATGCGCCCA	603	ATHAMRP	1820	112.016
545	ATGTTAAACAACACAATGATG	604	MLNNTMM	1821	111.939
546	ATTAGTTCGGGGATTTTGTCG	605	ISSGILS	1822	111.907
547	CGCCAAGGCAGCTTGATGATA	606	RQGSLMI	1823	111.83
548	ACGACTGATAAGGGTATTAAT	607	TTDKGIN	1824	111.818
549	CACAACTTAATGACCCAAATA	608	HNLMTQI	1825	111.77
550	AACCAAAACACCTACGAACTG	609	NQNTYEL	1826	111.756
551	GCTAACACCGTCACAGAACGA	610	ANTVTER	1827	111.7323
552	TCTACGCTGCAGACTAATGGT	611	STLQTNG	1828	111.683
553	CCCAACGAATACAAAGCACCG	612	PNEYKAP	1829	111.646
554	ATGCAAACACGCTCGGACACA	613	MQTRSDT	1830	111.629
555	GGAACAGGGTACGCTGGATCA	614	GTGYAGS	1831	111.6183
556	ATGGGTATGCAGAATACGCAT	615	MGMQNTH	1832	111.599
557	TCTAGTAAGGAGCGTACATCG	616	SSKERTS	1833	111.57
558	CGAACGGACACCCCCTACACC	617	RTDTPYT	1834	111.562
559	ACTGCGCTGCGGGATAATAAG	618	TALRDNK	1835	111.51
560	AGGATGTCTGAGAGTTCGGAT	619	RMSESSD	1836	111.51
561	AACCAATCTATAAGCATGGAC	620	NQSISMD	1837	111.491
562	TCGCTTGGGCATAGTAATAAT	621	SLGHSNN	1838	111.432
563	CTTAATAGTGGTGGTGCGATG	622	LNSGGAM	1839	111.361
564	AACGAACAATTCGAAAAAGTC	623	NEQFEKV	1840	111.341
565	ATGATGGCGAATAATATGCAG	624	MMANNMQ	1841	111.28
566	AGTCGGCGCGAAGAACAACCA	625	SRREEQP	1842	111.2512
567	GCGACTATGACTTCGTCGACG	626	ATMTSST	1843	111.238
568	CGTGGTTCAGACGGAGGATTG	627	RGSDGGL	1844	111.172
569	AGTTTGACGCCTAATAATCTT	628	SLTPNNL	1845	111.152
570	GCTACTCTTTCTCCGCATGCT	629	ATLSPHA	1846	111.132
571	TATCTGCAGGAGAAGTTTCCT	630	YLQEKFP	1847	111.112
572	GGCACCGGGTACCCAAACCAA	631	GTGYPNQ	1848	111.111
573	AATTATCCTTCGGTTCAGGAG	632	NYPSVQE	1849	111.07
574	ACTGACGCATCGGGTAGATCA	633	TDASGRS	1850	111.017
575	CGTGTGATTACTGCGGGTGAT	634	RVITAGD	1851	111.009
576	GTGACTGTGAGTAATAGTCTG	635	VTVSNSL	1852	110.95
577	TTGTTGACGGCTCCGCATAGG	636	LLTAPHR	1853	110.908
578	TCAATCGCAAACCACATGATA	637	SIANHMI	1854	110.861
579	ATGCCTTCGAAAGGCGAAGTA	638	MPSKGEV	1855	110.816
580	AACATGACCAACGAACGGCTC	639	NMTNERL	1856	110.801
581	TCATTCTCTTCAGGCATAATG	640	SFSSGIM	1857	110.771
582	CGCGACCGTCAAGACTCGGTA	641	RDRQDSV	1858	110.754
583	CACGGTGACCGAACAGCTTTA	642	HGDRTAL	1859	110.748
584	GAAGTACGGGGCAGCGTGCCA	643	EVRGSVP	1860	110.747
585	CTGATTTCGACTGGTAATAAT	644	LISTGNN	1861	110.735
586	CCAACATCTGGGGACAAACCG	645	PTSGDKP	1862	110.735
587	AAAGCGGACCACAGTGGGGCA	646	KADHSGA	1863	110.73
588	CTAAACGACGTCTACCGTAAA	647	LNDVYRK	1864	110.724
589	AACAGTTTGCAAGCAAGTGCA	648	NSLQASA	1865	110.72
590	TATCATAATGAGATTATGACG	649	YHNEIMT	1866	110.708
591	AACAACACCCTAAACATCCTA	650	NNTLNIL	1867	110.69
592	TCTTATGGGCAGGGTCTGGAG	651	SYGQGLE	1868	110.684
593	ATGATAAAAACCAACATGTTG	652	MIKTNML	1869	110.668
594	ACCGAAGCGGGCCGCCCCCAA	653	TEAGRPQ	1870	110.663
595	AGGATTGATCAGACTAATGTG	654	RIDQTNV	1871	110.624
596	GAGGGGCATAATCGTGGTATT	655	EGHNRGI	1872	110.559
597	ATGGGGACTGAGTATCGTATG	656	MGTEYRM	1873	110.524
598	TCGGGTATGAATAGTAATAAG	657	SGMNSNK	1874	110.499
599	TTGACTAATGATAATAAGTTG	658	LTNDNKL	1875	110.479
600	TTACACAACTACCAAGACCGT	659	LHNYQDR	1876	110.438
601	AAGTCTAATTTGGAGGGTAAG	660	KSNLEGK	1877	110.438
602	CTTACTGGTCAGAATGCGATT	661	LTGQNAI	1878	110.416
603	CATACTGTGGGGGCTATGCAT	662	HTVGAMH	1879	110.41
604	CTCCAACTGGCTACATCCCAC	663	LQLATSH	1880	110.384
605	AGTCTGAATGGGGTGTTGGTT	664	SLNGVLV	1881	110.359
606	AGTCACAACCAAGTAAACGTA	665	SHNQVNV	1882	110.349
607	AGTTTGAGTACTGATGTGTTT	666	SLSTDVF	1883	110.261
608	ATGGTAGGTCGTGCCGAAATC	667	MVGRAEI	1884	110.224
609	TTGTCTAGTATGAGTACGGAT	668	LSSMSTD	1885	110.204
610	TCCTACAGTACTTCAACACCG	669	SYSTSTP	1886	110.189
611	TCCGAATTAATGGTCAGACCC	670	SELMVRP	1887	110.0813
612	TGGAACGGAAACGCCACACAA	671	WNGNATQ	1888	110.039
613	ATGGATACTGAGCTTTATAGG	672	MDTELYR	1889	109.985
614	AGGACGAGTCCTGATACGAAT	673	RTSPDTN	1890	109.977
615	TTCTCAACGCAAGACATAAGC	674	FSTQDIS	1891	109.948
616	ACGACTGTGCTGGGGAATAAT	675	TTVLGNN	1892	109.94
617	CAGCGTGATGCTGCGTATGCT	676	QRDAAYA	1893	109.927
618	CACCAAACCGTGGTCCCTACT	677	HQTVVPT	1894	109.8948
619	TCTAATCCGGGTAATCATAAT	678	SNPGNHN	1895	109.853
620	TGGGAGACTATGGCTAAGCCT	679	WETMAKP	1896	109.818
621	GGTCTTTATCAGAATCCTACG	680	GLYQNPT	1897	109.73
622	CTTAATCTTACTAATCATAAT	681	LNLTNHN	1898	109.727
623	ATGAGTCTCGCCTCCACCCAA	682	MSLASTQ	1899	109.672
624	ACGTCCCAAACCGTCCGAGTA	683	TSQTVRV	1900	109.654
625	GGAGCAACGGTCAACACGCGA	684	GATVNTR	1901	109.64
626	AAAGGGGGAAACCTCACCGCA	685	KGGNLTA	1902	109.632
627	GCGTGGTCTCAAGTCCTGACG	686	AWSQVLT	1903	109.587
628	GTAGAACACGTAGCCCACCAA	687	VEHVAHQ	1904	109.552
629	CTAATGTCGTCCTACTCATCA	688	LMSSYSS	1905	109.546
630	TCTCTGGGTGGGAATCCGCCT	689	SLGGNPP	1906	109.511
631	AAGAATGAGAATACGAATTAT	690	KNENTNY	1907	109.5055
632	ATATTGGACAACCACCGTTTC	691	ILDNHRF	1908	109.489
633	AATTCGTCGCATGTTAATTCT	692	NSSHVNS	1909	109.473
634	CAGGTGCAGCATGAGAGGGTG	693	QVQHERV	1910	109.47
635	TTGGGAGGAACCCTGGGAATA	694	LGGTLGI	1911	109.46
636	ACTCAAGAACGACCACTAATC	695	TQERPLI	1912	109.455
637	CGTAAGACTGAGGATAGGATG	696	RKTEDRM	1913	109.429
638	ACCGAACTCACAGCGCGGAAC	697	TELTARN	1914	109.398
639	CGCGGCGACAACACTTACTCC	698	RGDNTYS	1915	109.387
640	CAGTCTAATACTAATAATAGT	699	QSNTNNS	1916	109.372
641	GCTTCTTATAGTATTTCTGAT	700	ASYSISD	1917	109.309
642	AGCGAACACCACGCCGGAATA	701	SEHHAGI	1918	109.281
643	CGTGGTGCGCCAGAGCATGCG	702	RGAPEHA	1919	109.237
644	AATTTTAGTAGTGGTGATGTT	703	NFSSGDV	1920	109.229
645	AGTGGCATCAACGCCACCGAC	704	SGINATD	1921	109.22
646	CGGGCTGATGTTTCTTGGTCT	705	RADVSWS	1922	109.213
647	TGTATGGATGTTGGTAAGGCG	706	CMDVGKA	1923	109.203
648	GGGGTCGGAGCCACTTCGGTA	707	GVGATSV	1924	109.193
649	AAAAACAACAACTCAGACAGT	708	KNNNSDS	1925	109.177
650	AATGTTGCGAGTATTGATAGG	709	NVASIDR	1926	109.174
651	AATAGTGTGAATGGTCTTCTG	710	NSVNGLL	1927	109.154
652	ACACTAGACCGAAACCAAACC	711	TLDRNQT	1928	109.132
653	GACCAAAACTTCGAACGTAGA	712	DQNFERR	1929	109.108
654	GTCGGTGACAGGAACTTGGTC	713	VGDRNLV	1930	109.062
655	TTAGAAGTAAACCTGCAAACG	714	LEVNLQT	1931	109.057
656	ACTAATGGGGGGTCGCTTAAT	715	TNGGSLN	1932	109.049
657	TTCACGCGCACACCAGTAACC	716	FTRTPVT	1933	109.033
658	ACACCGGCGGAAAGCAAAGTT	717	TPAESKV	1934	108.991
659	TTTCCTTCGCATAATGGGGCG	718	FPSHNGA	1935	108.959
660	GCCAGGAACGTAATGCTGGGG	719	ARNVMLG	1936	108.958
661	ACGATTCAGGATCATATTAAG	720	TIQDHIK	1937	108.942
662	ATTAATTCGTATTTGCATGAG	721	INSYLHE	1938	108.918
663	GCGCATGATGTTACTGTGAAT	722	AHDVTVN	1939	108.918
664	ACTGTGGGGGTTCAGCAGACG	723	TVGVQQT	1940	108.8891
665	ACAGGTAGTTCAGACAGATTA	724	TGSSDRL	1941	108.887
666	AATCATGATACTGCTCATGCT	725	NHDTAHA	1942	108.884
667	GCCGAATCCCAACTAGCTAGC	726	AESQLAS	1943	108.8752
668	GGTAATGCGTATAATACGACT	727	GNAYNTT	1944	108.818
669	AATCATCAGGCTGGTACTACT	728	NHQAGTT	1945	108.807
670	ACGGTAGGAGAAAACCACCGA	729	TVGENHR	1946	108.779
671	CTAACTACTAAAATACCCCTC	730	LTTKIPL	1947	108.773
672	ACTAATTATCCTGAGGCGAAT	731	TNYPEAN	1948	108.748
673	AATACTGCTCCGCCGAATCAT	732	NTAPPNH	1949	108.733
674	GTGCTGAGTACGGGGCTGCGG	733	VLSTGLR	1950	108.677
675	CTCACGTCCCACTCTGCGGGC	734	LTSHSAG	1951	108.648
676	ATGAATAAGCATGGTGTGCTT	735	MNKHGVL	1952	108.5736
677	GACCTGACCAGAGCTGCAATA	736	DLTRAAI	1953	108.552
678	TATATTGTGGATCATGCGAAT	737	YIVDHAN	1954	108.526
679	AGTGGGCCTGAGAATACGTTG	738	SGPENTL	1955	108.526
680	CGTTATGGTGATACGGGTATG	739	RYGDTGM	1956	108.512
681	GATGGTAAGAATAGTTATGCG	740	DGKNSYA	1957	108.451
682	GAGGCGCATAATCGTGTTATT	741	EAHNRVI	1958	108.451
683	AGTTTGCAGGCTGGTAGGATG	742	SLQAGRM	1959	108.3681
684	GATGCGAAGGCTCTTACGACT	743	DAKALTT	1960	108.368
685	ACCGACACCCGAAAAAACGAC	744	TDTRKND	1961	108.357
686	GACTCTTCACACTACTCGACA	745	DSSHYST	1962	108.219
687	ACAATGCACCTTCCCAACCTG	746	TMHLPNL	1963	108.214
688	CGAGACGGCTCTACTAAAGTT	747	RDGSTKV	1964	108.207
689	TCAGGGTACCAAATGACAGAA	748	SGYQMTE	1965	108.16
690	TGCGACTTGTCACAATCATGC	749	CDLSQSC	1966	108.133
691	AGAAACGCGTCAAACGGCGTA	750	RNASNGV	1967	108.044
692	CAGTCGCAGAATGTGACTCAG	751	QSQNVTQ	1968	108.033
693	GATTCTGCTCCGAGTACTATT	752	DSAPSTI	1969	108.003
694	AGGTCCGTACCATCACCACAC	753	RSVPSPH	1970	108.001
695	ATGACGTCTGCGTCTCGTGGT	754	MTSASRG	1971	107.974
696	GCTCTTGCTAGTCGTCCTATG	755	ALASRPM	1972	107.907
697	CTAAACCTCTCCAACGACTGG	756	LNLSNDW	1973	107.899
698	GTTTCTACGGCGCAGAGGCAG	757	VSTAQRQ	1974	107.896
699	CACGCCGACGTTGGCATGAGC	758	HADVGMS	1975	107.888
700	GCGGGGGGTTTGCTGTCGCGG	759	AGGLLSR	1976	107.878
701	CATCTTAGTCAGGCTAATCAT	760	HLSQANH	1977	107.848
702	GTGCATAATCCTACTACTACG	761	VHNPTTT	1978	107.8152
703	TCTCAGCGGAATCCGGATGAT	762	SQRNPDD	1979	107.784
704	AGGGAGACTAATAATTTTGCG	763	RETNNFA	1980	107.771
705	AATGCGGGGGCTCTTATGGGT	764	NAGALMG	1981	107.764
706	TTGCCGAAGACTGTGAATATG	765	LPKTVNM	1982	107.738
707	GCAAGTGACCTACAAATGACG	766	ASDLQMT	1983	107.723
708	CAAGCCCTGGCCACCACAAAC	767	QALATTN	1984	107.716
709	CATGAGTCGTCTGGTTATCAT	768	HESSGYH	1985	107.696
710	GGGGTGAATGATCGTGCTAGG	769	GVNDRAR	1986	107.69
711	CCTCGGGATGCTCTTCGTACT	770	PRDALRT	1987	107.673
712	AACGACTCCTCGTCAATGTCC	771	NDSSSMS	1988	107.641
713	GAATACAACACGCGCCACGAC	772	EYNTRHD	1989	107.611
714	GCGTCTCCGGCGCATACGTCT	773	ASPAHTS	1990	107.598
715	CAAAACAGCAACACTCCCTCA	774	QNSNTPS	1991	107.546
716	TTGGCAAAACTAGGGAACTAC	775	LAKLGNY	1992	107.541
717	GCTAGTGATAGGCAGTCTGGT	776	ASDRQSG	1993	107.527
718	TATCAGAATGGTGTGCTTCCT	777	YQNGVLP	1994	107.5199
719	AATAAGTTTGGTTATAATCCT	778	NKFGYNP	1995	107.513
720	AAAAAAACCAACGGAATCCCC	779	KKTNGIP	1996	107.5
721	GTTAACGACAACCGAGGAAAC	780	VNDNRGN	1997	107.4937
722	ATGCACACCATAACGGGATCC	781	MHTITGS	1998	107.491
723	ATTGATGGTGTTCAGAAGCTT	782	IDGVQKL	1999	107.489
724	GCGCAGGTTAATAATCATGAT	783	AQVNNHD	2000	107.489
725	GTTTCTTCGCCTAATGGTACG	784	VSSPNGT	2001	107.487
726	GATTCTGCTCCGAGGGCTATT	785	DSAPRAI	2002	107.455
727	TCTGCGAGTGATAGTCAGCAT	786	SASDSQH	2003	107.455
728	TCGGCTCATCAGACGCCGACG	787	SAHQTPT	2004	107.427
729	GCGACGCTGAATAATAGTTAT	788	ATLNNSY	2005	107.411
730	GAAGACAGTATGAGATTCTCT	789	EDSMRFS	2006	107.407
731	GAACGAAACGGACTAATAGAA	790	ERNGLIE	2007	107.405
732	TTAGTACTTGACTCACGGAAC	791	LVLDSRN	2008	107.382
733	ACCGTCGAACAAATAAACTCG	792	TVEQINS	2009	107.349
734	GGGACAGGTACCGTTGGATGG	793	GTGTVGW	2010	107.203
735	AATCAGCAGCGTATTGATAAT	794	NQQRIDN	2011	107.185
736	ATCCAAAACGGGGTCCTGCCA	795	IQNGVLP	2012	107.184
737	GGAGACATCTCAAGCAGAAAC	796	GDISSRN	2013	107.1386
738	GTCACTGGCACTACCCCGGGA	797	VTGTTPG	2014	107.137
739	ACAAGGGAATCAATGTCCATC	798	TRESMSI	2015	107.071
740	CACACTTACTCACAAGCAGAC	799	HTYSQAD	2016	107.012
741	TCCAACATGGGCGTAGCCTCT	800	SNMGVAS	2017	106.985
742	CACGACTTGAACCACGGAAAA	801	HDLNHGK	2018	106.942
743	CTGTACGGGGGAGCACACCAA	802	LYGGAHQ	2019	106.904
744	AACGTGTACGGAGACGGAATA	803	NVYGDGI	2020	106.87
745	TCTACTATTAATATGCGTGCG	804	STINMRA	2021	106.868
746	AAGATGGGGAGTATTGAGGTT	805	KMGSIEV	2022	106.864
747	TCCGAAACGCGCGCTGGATAC	806	SETRAGY	2023	106.85
748	AATGTGGGTAATACTCTTGGG	807	NVGNTLG	2024	106.842
749	ATTGGTGGGACTGATACGCGG	808	IGGTDTR	2025	106.786
750	GCCGACAAAGGATTCGGCCAC	809	ADKGFGH	2026	106.73
751	TGGCAGGATCATAATAAGGTG	810	WQDHNKV	2027	106.719
752	AACTACGGTTCCGGACGAATC	811	NYGSGRI	2028	106.701
753	ACTCATAAGCAGGTGGATCTT	812	THKQVDL	2029	106.695
754	CGGCAGAATGATAAGGGTAAT	813	RQNDKGN	2030	106.658
755	GGTAGGAATGAGAGTCCGGAG	814	GRNESPE	2031	106.658
756	GTTTTTACTGGGCAGACGGAG	815	VFTGQTE	2032	106.632
757	TATGTTGATCGTAAGGATAAT	816	YVDRKDN	2033	106.631
758	AATAATACTTTGAATATTTTG	817	NNTLNIL	2034	106.63
759	TTGAGCTACAGCATCCAACAC	818	LSYSIQH	2035	106.621
760	GCTACCAACAGATCGCCCCTA	819	ATNRSPL	2036	106.5898
761	GTTCACACCGCAGACACAATA	820	VHTADTI	2037	106.564
762	GGGCATTTGGTTAATATGTCT	821	GHLVNMS	2038	106.56
763	TTAGACTACACCCCTCAAAAC	822	LDYTPQN	2039	106.519
764	TCCGCCTCTTACTCCAGGATG	823	SASYSRM	2040	106.501
765	TCCGGAGCGGCACAAAACCCA	824	SGAAQNP	2041	106.499
766	AGAAACACACTTGCTGACCTT	825	RNTLADL	2042	106.496
767	GGTTCTACGGTGTCGGCGCAG	826	GSTVSAQ	2043	106.491
768	TCTAAGGATAGTACTATGTAT	827	SKDSTMY	2044	106.48
769	GTGGTGGTTCACACTATCCCA	828	VVVHTIP	2045	106.45
770	CCACGTACTGTCTCATTGGAC	829	PRTVSLD	2046	106.4434
771	ATGATGAAGAGTGAGGAGAAT	830	MMKSEEN	2047	106.425
772	ACCACCGACCGGCCAAACGGA	831	TTDRPNG	2048	106.406
773	CATAGTCCTCCTACGACTATG	832	HSPPTTM	2049	106.376
774	GGCCAATGGACAACAGGGACA	833	GQWTTGT	2050	106.357
775	GACGGTATGAACGGAGTGGGT	834	DGMNGVG	2051	106.317
776	CTTCATACTGTTGCGAATGAG	835	LHTVANE	2052	106.312
777	TATACGTCGCAGACGTCTACG	836	YTSQTST	2053	106.2842
778	AACTTCTCCGAAATGTCCACA	837	NFSEMST	2054	106.27
779	ATTAATATTCGTAGTGATTTG	838	INIRSDL	2055	106.266
780	CCCTCCAACAGTGAAAGATTC	839	PSNSERF	2056	106.249
781	TATACGAATTATGGGGATCTT	840	YTNYGDL	2057	106.241
782	GATAAGAGTACGGCGCAGGCG	841	DKSTAQA	2058	106.238
783	CACACCGACATGGTATCCTCT	842	HTDMVSS	2059	106.222
784	AACAAAAGTCTGTCAATGGAC	843	NKSLSMD	2060	106.196
785	GGGCACTACGCTACAAACACA	844	GHYATNT	2061	106.158
786	GTCATCGTATCTACAAAATCA	845	VIVSTKS	2062	106.124
787	ACTCATAGTCTTATGAATGAT	846	THSLMND	2063	106.116
788	AACTACCACGGAGACAACGTT	847	NYHGDNV	2064	106.106
789	CGTGATGATCAGCAGCTTGAT	848	RDDQQLD	2065	106.064
790	GATGATAAGACTGGTCGGTAT	849	DDKTGRY	2066	106.055
791	GGGTCGAGCCAACACCACGAA	850	GSSQHHE	2067	106.042
792	CGTGTTACAGGTGTCTCAACA	851	RVTGVST	2068	106.017
793	AGTACTGCGTCGGGGCATACT	852	STASGHT	2069	106.007
794	ACTAACAACCTCTCATACGAA	853	TNNLSYE	2070	105.998
795	CAGCATAATAGTGCGTCGGCG	854	QHNSASA	2071	105.987
796	CCGGCTAAGGGTTTTGGTCAT	855	PAKGFGH	2072	105.9781
797	TGGTACGAAACAATCAGCCCG	856	WYETISP	2073	105.959
798	ACGGATGCTACGGGGAGGCAT	857	TDATGRH	2074	105.942
799	ATTCAGGCGAAGAATTCTGAG	858	IQAKNSE	2075	105.939
800	AGTACTGAGACTAGGGGTGGG	859	STETRGG	2076	105.926
801	TTCTCAACAAACTCTGTAATC	860	FSTNSVI	2077	105.918
802	TCTAACCTTCGAAACACAATA	861	SNLRNTI	2078	105.854
803	GGGATGATCGGGCACAACGCA	862	GMIGHNA	2079	105.832
804	TCTGGCCAAGGATTCTCGGCA	863	SGQGFSA	2080	105.831
805	ACCCACAACTCTACAGGCCTT	864	THNSTGL	2081	105.802
806	AGGATTGATAGTGCTATGGTG	865	RIDSAMV	2082	105.8
807	GTCGCTATGGGAGGCGGTCCC	866	VAMGGGP	2083	105.795
808	GGCTCTCACAACGGCCCAGCC	867	GSHNGPA	2084	105.763
809	CACTCCGCAGCGGGTGACGGT	868	HSAAGDG	2085	105.73
810	GCACAAGGCATAACCCACGCT	869	AQGITHA	2086	105.711
811	TCTGCGCTTTTGCGGATGGAT	870	SALLRMD	2087	105.707
812	TGGCAAATGGGGGCCGGGAGC	871	WQMGAGS	2088	105.698
813	ATAGACTCGCACGCCAGCATA	872	IDSHASI	2089	105.695
814	AGCCTAGACCACGCCCCTCTA	873	SLDHAPL	2090	105.661
815	GAAAACAACATGCAACACGGC	874	ENNMQHG	2091	105.651
816	AAGGGTGCGCAGGGTGTTCAG	875	KGAQGVQ	2092	105.646
817	GTCGCTGTATCGAACACTCCA	876	VAVSNTP	2093	105.643
818	GTTGAGTCTTCTTATTCTCGG	877	VESSYSR	2094	105.633
819	CATAATACGGAGTCTAAGACT	878	HNTESKT	2095	105.625
820	AATGAGAGTACGAAGGAGAGT	879	NESTKES	2096	105.599
821	GATGTTTATCTTAAGAGTCCG	880	DVYLKSP	2097	105.586
822	CAGTCGGGGGCTAGGACTCTG	881	QSGARTL	2098	105.5854
823	TCGAACAGTCAAGTACACAAC	882	SNSQVHN	2099	105.573
824	GTAGTCTCATCGGGCGGCTGG	883	WSSGGW	2100	105.551
825	CCATCAAGTTTCAACAGCGCC	884	PSSFNSA	2101	105.542
826	AAGCAGACTGATAGTAGGGGT	885	KQTDSRG	2102	105.5
827	AACACAACGCCACCTAACCAC	886	NTTPPNH	2103	105.483
828	CAAAACGGAACCTCGTCTATA	887	QNGTSSI	2104	105.483
829	CTCATGAAAGACATGGAATCC	888	LMKDMES	2105	105.458
830	ACTCAGACTGGTCATGTTTCT	889	TQTGHVS	2106	105.4558
831	GAAATACACACGACCACAGGC	890	EIHTTTG	2107	105.449
832	ATACAAACTACTACAAAATGC	891	IQTTTKC	2108	105.442
833	CCCGCTGAAGGAAACAACCGT	892	PAEGNNR	2109	105.442
834	TACATCGCCGGAGGGGAACAA	893	YIAGGEQ	2110	105.415
835	GAAGTACGCGACCAAAAAACA	894	EVRDQKT	2111	105.375
836	TACGCCGTCGCGATAGGCACA	895	YAVAIGT	2112	105.366
837	TCCGCTAACGAACACAACCAC	896	SANEHNH	2113	105.337
838	GGGATGAGGGATACGCCGCCG	897	GMRDTPP	2114	105.322
839	GCTCAGCAGATTGTTAATGGG	898	AQQIVNG	2115	105.321
840	TCAAGTTCCCAAACGGTTTTG	899	SSSQTVL	2116	105.321
841	GTTATTCAGTCTGATAATACG	900	VIQSDNT	2117	105.32
842	GTTCCGGCGCATTCTCGGGGT	901	VPAHSRG	2118	105.305
843	TCGAATACGGGGTCGTTGGGT	902	SNTGSLG	2119	105.2779
844	TGGGCCAAAGACGTCAACGTC	903	WAKDVNV	2120	105.273
845	AATGTGTTGGGTGCTTCGAGT	904	NVLGASS	2121	105.187
846	ACTCCGGAGGCTAGTGCGCGT	905	TPEASAR	2122	105.173
847	AATTATAATGGGGTTAATGTG	906	NYNGVNV	2123	105.152
848	AACACAACCGGTAGCTCGGGC	907	NTTGSSG	2124	105.145
849	TCCAGCGGCCAACCGCTCGTC	908	SSGQPLV	2125	105.136
850	CAGGCGGGGGGTGTGGCGAGT	909	QAGGVAS	2126	105.133
851	CCGCTTCAATCCCAATCGGGA	910	PLQSQSG	2127	105.133
852	CAACGTACCTCGGAAGCGCCA	911	QRTSEAP	2128	105.128
853	TTGGCTAAGACGGTTGCGATT	912	LAKTVAI	2129	105.1155
854	ACCCACACCCTTGGGGGAACA	913	THTLGGT	2130	105.08
855	CACGACTACAGTATGAACGCG	914	HDYSMNA	2131	105.079
856	GGGAAACCTGCGGAAGCGCCG	915	GKPAEAP	2132	105.055
857	AGAAACGAAAACGTAAACGCT	916	RNENVNA	2133	105.051
858	AGTTCTCGGGAGGCGAAGTTT	917	SSREAKF	2134	105.0379
859	TCTTCTTCTGATAGTCCGCGT	918	SSSDSPR	2135	105.035
860	ATGAATACGACTTATAATGAG	919	MNTTYNE	2136	105.031
861	GTAAGGAGTGGAATAAAACCA	920	VRSGIKP	2137	105.008
862	CAGGAGAATCCTATGAAGATG	921	QENPMKM	2138	104.926
863	ACTGAGCCGCTTCCGATGTCT	922	TEPLPMS	2139	104.869
864	CGCCACGGGGACACACCGATG	923	RHGDTPM	2140	104.844
865	GCGGTGAATACGTATAATAGT	924	AVNTYNS	2141	104.82
866	GCGTCGACTGAGTCTCATGTG	925	ASTESHV	2142	104.816
867	ACAAACCTAAGTCAATCGGCC	926	TNLSQSA	2143	104.791
868	GAGCTGTCTACTCCTATGGTT	927	ELSTPMV	2144	104.783
869	TATGCGCATCCTGTGACTCAT	928	YAHPVTH	2145	104.76
870	CGGGGGTCTACTGGTACGCAG	929	RGSTGTQ	2146	104.749
871	TGTGTTGGTTCGTGTGGTGTG	930	CVGSCGV	2147	104.738
872	TCGGTTGCTAAGGATCAGACG	931	SVAKDQT	2148	104.736
873	ACGAATCTTTCTCCTAAGACG	932	TNLSPKT	2149	104.6855
874	CTAGGTTTCACACCCCAACCG	933	LGFTPQP	2150	104.677
875	AATATTAGTAGTATTAATCAG	934	NISSINQ	2151	104.657
876	GTTTACGACAACGTTTCTTCT	935	VYDNVSS	2152	104.657
877	AGTGGAAAACAAGACAAATAC	936	SGKQDKY	2153	104.654
878	AGACTTACAGAACTGGTCATA	937	RLTELVI	2154	104.651
879	CATAAGAGTGAGAGTCATAAT	938	HKSESHN	2155	104.626
880	GAGGCGACTCATGGTTCTTAT	939	EATHGSY	2156	104.613
881	AACCTACTTGTCGACCAACGT	940	NLLVDQR	2157	104.579
882	AATATTAATGATACTAAGAAT	941	NINDTKN	2158	104.522
883	CTTGCGGTTACGAATGTGCGG	942	LAVTNVR	2159	104.498
884	CCGTCGACACTCGCTGAAACA	943	PSTLAET	2160	104.449
885	CCGAAGCCTGGGACGGGGGAG	944	PKPGTGE	2161	104.427
886	GTGCTGTTGCAGAATTCTCAT	945	VLLQNSH	2162	104.416
887	TACGGTAACGCGAACACCGTA	946	YGNANTV	2163	104.386
888	ACATCTGGAGTTCTGACACGC	947	TSGVLTR	2164	104.375
889	AAAATAACGGAAACCAACCTC	948	KITETNL	2165	104.359
890	GTTCGCAGAGACGAAACACCT	949	VRRDETP	2166	104.359
891	TCTAAAATGTCAAACCCAGTG	950	SKMSNPV	2167	104.352
892	TGGGAATCCCTCTCCAACGCA	951	WESLSNA	2168	104.349
893	GCCAACGGAGGAGGATACCCC	952	ANGGGYP	2169	104.34
894	ATGTTGGCTTCTCGGGTGCCT	953	MLASRVP	2170	104.336
895	TGCGGCCTGAACTGCGGTAAA	954	CGLNCGK	2171	104.331
896	ACTATTACTAGTCCGTCGGTG	955	TITSPSV	2172	104.3055
897	TGGTCGAATGCTCAGAGTCCG	956	WSNAQSP	2173	104.288
898	ACAGAAAGCCCCAAACTACTA	957	TESPKLL	2174	104.283
899	CATTTGGTTACTAGTGGTATT	958	HLVTSGI	2175	104.273
900	CCTCCTAAGTCGGATTCGAAT	959	PPKSDSN	2176	104.265
901	ATTGCGGTGCATGTGCTGAGT	960	IAVHVLS	2177	104.254
902	ACTGGTACTGCGACTTTGCCT	961	TGTATLP	2178	104.254
903	AATACTACTCCGCCTAATCAT	962	NTTPPNH	2179	104.232
904	TGCACCGCCACAAAATGCTCA	963	CTATKCS	2180	104.23
905	CACAGTGACATGGTCAGCGGC	964	HSDMVSG	2181	104.208
906	CCAAACGCACACCACCTGCCC	965	PNAHHLP	2182	104.2
907	TCTAATAATATGAATCAGGCG	966	SNNMNQA	2183	104.187
908	AGTGATAATAATAGGGCTAAT	967	SDNNRAN	2184	104.1865
909	TTGCAGACGCCTGGGACGACG	968	LQTPGTT	2185	104.169
910	GTGCGCGGCGTTCAAGACGCC	969	VRGVQDA	2186	104.167
911	TCTCTAGACTCGCGCTCCTCG	970	SLDSRSS	2187	104.14
912	GTTTGTGTTACTACTTGTGCT	971	VCVTTCA	2188	104.137
913	CCGAATACTAATCATCTTGTG	972	PNTNHLV	2189	104.121
914	CTCATGTCAGGGAAAGAAAAC	973	LMSGKEN	2190	104.109
915	ACTTCTGCTAGTGAGAATTGG	974	TSASENW	2191	104.108
916	TTTTTGCCGCAGCTGGGGCAG	975	FLPQLGQ	2192	104.094
917	CCTTTTAATCCTGGGAATGTG	976	PFNPGNV	2193	104.0922
918	GGGACACCTGGTCAAAGTATA	977	GTPGQSI	2194	104.092
919	TATAATAATGGTGGGCATGTT	978	YNNGGHV	2195	104.085
920	CTCGGAAACCACTACACACCC	979	LGNHYTP	2196	104.064
921	CAAGTCAACCAACCGAGAATA	980	QVNQPRI	2197	104.061
922	TTAGGAAACAACCGGCCACTA	981	LGNNRPL	2198	104.06
923	CCTCCGGAAAGTGCCAGGGGC	982	PPESARG	2199	104.023
924	AAATCTGTAGGCGACGGGAGA	983	KSVGDGR	2200	104.0009
925	TCACTTCGGACGGACGAATTC	984	SLRTDEF	2201	103.997
926	AGTACTACTAATGTTGCGTAT	985	STTNVAY	2202	103.987
927	AGGATGTCGGATCCTAGTGAT	986	RMSDPSD	2203	103.981
928	AGTCTGTCTATTACTTCGGCG	987	SLSITSA	2204	103.963
929	GAAAGTGCCACATCTCTAAAA	988	ESATSLK	2205	103.954
930	TACACTGACGGAAGAAACACC	989	YTDGRNT	2206	103.949
931	TCCATATCCAACCTGCGTACC	990	SISNLRT	2207	103.935
932	CAAAACGACAAATCTGACAAC	991	QNDKSDN	2208	103.9165
933	GGTGGAACAGGTCTTTCCAAA	992	GGTGLSK	2209	103.916
934	AGTCAGGCTCAGATTCGTGTT	993	SQAQIRV	2210	103.915
935	GGTTTGATGGCGCATGTGACT	994	GLMAHVT	2211	103.877
936	CTGGTTGTTTCGAATAGTCTG	995	LWSNSL	2212	103.865
937	CATGATTCTGTGAATACGGCG	996	HDSVNTA	2213	103.8588
938	ACTCTTGCGAAGGATGGGAAT	997	TLAKDGN	2214	103.842
939	TCCGACGGATCGAAACTACTA	998	SDGSKLL	2215	103.829
940	ATAGACAAAACGTTCTCGGTC	999	IDKTFSV	2216	103.812
941	CGGCTGGTTAACATCGACCAC	1000	RLVNIDH	2217	103.8026
942	AAAAACTACGACAGTGACTCA	1001	KNYDSDS	2218	103.794
943	AGTACGCAGAGTACTAATCCG	1002	STQSTNP	2219	103.7868
944	CAAATATCACTACAACTCGGC	1003	QISLQLG	2220	103.77
945	TCCGAACCCCTTAGAGTTGGA	1004	SEPLRVG	2221	103.749
946	AGTCGTCTGCAGACTCAGCAG	1005	SRLQTQQ	2222	103.7406
947	GAAGGTTCACAAGGAAACCAC	1006	EGSQGNH	2223	103.739
948	CGTTCTGACCTTACTGAAAGT	1007	RSDLTES	2224	103.736
949	CATACTGGTGTTCAGACTAAT	1008	HTGVQTN	2225	103.724
950	GAGTTGGATCATCTTTCGCAT	1009	ELDHLSH	2226	103.714
951	GTTACTGGTGTTGATTATGCG	1010	VTGVDYA	2227	103.713
952	GGCGGCGCACACACTCGTGTA	1011	GGAHTRV	2228	103.676
953	GCCTACGGTATACACGAAGTG	1012	AYGIHEV	2229	103.653
954	GCGATGCTGCGTATGGAGCAG	1013	AMLRMEQ	2230	103.652
955	AGGCAGGCGAATCAGACGTAT	1014	RQANQTY	2231	103.652
956	TTTTCTGGTCAGGCGTTGGCT	1015	FSGQALA	2232	103.646
957	GATAATGTGAATTCTCAGCCT	1016	DNVNSQP	2233	103.646
958	GGGTTGCATGGGACGAGTAAT	1017	GLHGTSN	2234	103.633
959	GAGAGGGAGCCTCCTAAGAAT	1018	EREPPKN	2235	103.621
960	GTGGTGACGCTTGGGATGCTG	1019	VVTLGML	2236	103.619
961	CATAATAATAATTTGCTGAAT	1020	HNNNLLN	2237	103.612
962	TTGATTAATATGAGTCAGAAT	1021	LINMSQN	2238	103.6
963	AATACTAATGCGTCGTATTCT	1022	NTNASYS	2239	103.599
964	AGGCTTAATGCGGGTGAGCAT	1023	RLNAGEH	2240	103.594
965	GCTGTTATTCTGAATCCTGTT	1024	AVILNPV	2241	103.576
966	CCGAGTACTCATGGGTATGTT	1025	PSTHGYV	2242	103.571
967	CTTAGGGCGTCTGTGTCGGAG	1026	LRASVSE	2243	103.564
968	ATGATGACCTCTATGACGTTA	1027	MMTSMTL	2244	103.561
969	TCGGCACACAACATAGTATAC	1028	SAHNIVY	2245	103.556
970	CACGACAGCACAACCCGCCCA	1029	HDSTTRP	2246	103.545
971	ATCAAAGACTCGTACCTTACT	1030	IKDSYLT	2247	103.542
972	TATACGCCTGGGCTTACTGAG	1031	YTPGLTE	2248	103.541
973	AAGATGGGTGGTTCTCAGAGT	1032	KMGGSQS	2249	103.477
974	TCACGTCAAACAGCGCTAACA	1033	SRQTALT	2250	103.4599
975	GTAGAAACCAGCAGATTGTAC	1034	VETSRLY	2251	103.45
976	AAATCCAACAACGGGGAATAC	1035	KSNNGEY	2252	103.424
977	TCGGGTGTTCATAGTGCGCGT	1036	SGVHSAR	2253	103.3881
978	CCTAACAACGAAAAAAACCCG	1037	PNNEKNP	2254	103.326
979	ACTATTGGTGAGGGGTATCAT	1038	TIGEGYH	2255	103.325
980	CTGCAGACTTCTGTTGCTACT	1039	LQTSVAT	2256	103.316
981	CTATTGGGAAACGCACCCACA	1040	LLGNAPT	2257	103.308
982	ATTTCGGGGTCTCATTTGAAT	1041	ISGSHLN	2258	103.297
983	AAGTCTCTTAGTAGTGATGAT	1042	KSLSSDD	2259	103.285
984	ACGAGGACTCAGGGGACGTCT	1043	TRTQGTS	2260	103.2635
985	GTTAGTAGGTCTGGGAGTACT	1044	VSRSGST	2261	103.257
986	AGCGCCGACACCCGGTCCCCC	1045	SADTRSP	2262	103.242
987	CGTGATACTGCTAATGGGCCG	1046	RDTANGP	2263	103.2389
988	ATGATGTCTAACAGCCTCGCG	1047	MMSNSLA	2264	103.232
989	ACTGGGAGGATTGAGCTTAGG	1048	TGRIELR	2265	103.214
990	GCTAATAATGCGGCTGCGTCG	1049	ANNAAAS	2266	103.209
991	CAGTTGAATATTAATGATAAG	1050	QLNINDK	2267	103.208
992	ATGGACGGGGCTCACACGTCA	1051	MDGAHTS	2268	103.202
993	ACTAGTGCGACTGATTCGATG	1052	TSATDSM	2269	103.197
994	GCCGCCAGCTTGTCGCAAAGC	1053	AASLSQS	2270	103.152
995	TCTCAGGCGGGTCTGCTTGTG	1054	SQAGLLV	2271	103.116
996	ACGACTTATTCGGATCTGAGT	1055	TTYSDLS	2272	103.104
997	TTCTCCTCCGGAACAACCATA	1056	FSSGTTI	2273	103.102
998	GTCTTCACAGAAATAGAATCG	1057	VFTEIES	2274	103.101
999	GCAGACCCCGCTAAAGGCAAA	1058	ADPAKGK	2275	103.083
1000	AAAGAATCTGAATACAGAGTT	1059	KESEYRV	2276	103.07
1001	GGGATGGTGTCTCTTAATAGG	1060	GMVSLNR	2277	103.06
1002	ACCGTTATCGAACGCAAAGAC	1061	TVIERKD	2278	103.0575
1003	AGGATTGATACGTTGTTGGTG	1062	RIDTLLV	2279	103.055
1004	GGATCCACAGGCCTACCCCCG	1063	GSTGLPP	2280	103.047
1005	ATGGAGTTGACTTCTACTAGT	1064	MELTSTS	2281	103.026
1006	CAACCAGGAGCCCCCCAAACC	1065	QPGAPQT	2282	103.014
1007	AATTCGATGGGTAATGGGGGT	1066	NSMGNGG	2283	103.009
1008	GGTAGTACTAAGTCTGGGCAG	1067	GSTKSGQ	2284	103.0049
1009	ACTTTTTTGCCTCAGCTTGGG	1068	TFLPQLG	2285	102.994
1010	ATGGGAATAAACGTACTGAGC	1069	MGINVLS	2286	102.986
1011	GTGAATCTTGGTATTTCGGGG	1070	VNLGISG	2287	102.985
1012	AGTGAGAATCGGGCTGGTAAT	1071	SENRAGN	2288	102.945
1013	CACTCCAACGCGACTACGATA	1072	HSNATTI	2289	102.916
1014	CCGGGGTCGTCCGCTTCCATC	1073	PGSSASI	2290	102.914
1015	ATTACGTCGTTGAATGGGATG	1074	ITSLNGM	2291	102.909
1016	TATCTGGAGGGTGCTCATCGT	1075	YLEGAHR	2292	102.896
1017	AGGCAGGTTGAGCAGTCTGAT	1076	RQVEQSD	2293	102.889
1018	AGCTCTCAAAGTTCCGGGTCG	1077	SSQSSGS	2294	102.8836
1019	CAGCTTACTGTTGGGAAGCCG	1078	QLTVGKP	2295	102.8762
1020	GTTGTGCATTCGAGTATTACT	1079	WHSSIT	2296	102.8257
1021	CTAGAACAACTACGGGTCCCA	1080	LEQLRVP	2297	102.815
1022	CAGCATTCTCCGAAGCCGGTT	1081	QHSPKPV	2298	102.81
1023	GCGGGCAGTTCGCCATCACGC	1082	AGSSPSR	2299	102.8035
1024	GGAGTAACAATCGGTAGCAGG	1083	GVTIGSR	2300	102.7752
1025	TACATCGCGGGAGGCGACCAA	1084	YIAGGDQ	2301	102.75
1026	ATTAGTAGTGAGAGGTTTTCT	1085	ISSERFS	2302	102.729
1027	AGGAGTGAGGGTAATCATGCT	1086	RSEGNHA	2303	102.719
1028	GAGAAGGGGAATAGTGGGGTT	1087	EKGNSGV	2304	102.71
1029	TACATAGTTGACCACGCTAAC	1088	YIVDHAN	2305	102.71
1030	CGTCGGTTGAGTACGGATCTT	1089	RRLSTDL	2306	102.702
1031	GCGAATAGTAGGCTTGGGGCG	1090	ANSRLGA	2307	102.6979
1032	GGTACTGCTGAGAATACGAGT	1091	GTAENTS	2308	102.696
1033	GTGAGGGATGTTGCTAAGGAG	1092	VRDVAKE	2309	102.691
1034	GGAGGCCTTACCAACGGTCTA	1093	GGLTNGL	2310	102.67
1035	CCTTCGATTCCGTCGTTTTCG	1094	PSIPSFS	2311	102.657
1036	AACGCTCTCCTCAACGCACCT	1095	NALLNAP	2312	102.628
1037	GACGACATGGTCAAAAACTCA	1096	DDMVKNS	2313	102.623
1038	ACTGCGAATACGCATGCTCTG	1097	TANTHAL	2314	102.613
1039	GTATACGCCACCGCACTCGCA	1098	VYATALA	2315	102.611
1040	GGTATATACCCGGCATCCACC	1099	GIYPAST	2316	102.61
1041	GGTTTTGATGGTAAGCAGCTT	1100	GFDGKQL	2317	102.606
1042	CACTCTATGTCCGCAAACACC	1101	HSMSANT	2318	102.605
1043	TGGAGCATCAAAAACCAAACA	1102	WSIKNQT	2319	102.586
1044	ACCCTCCACACCAAAGACCTA	1103	TLHTKDL	2320	102.57
1045	TCTTATGGTAATACTCATGAT	1104	SYGNTHD	2321	102.566
1046	CAGTCGGGGTCTCTGGTGCCG	1105	QSGSLVP	2322	102.552
1047	AATACTTTGCAGAATAGTCAT	1106	NTLQNSH	2323	102.5506
1048	ACGGCTGAGTCTAGTCATCCG	1107	TAESSHP	2324	102.548
1049	GCCTCTACAGTCTCACTCTAC	1108	ASTVSLY	2325	102.547
1050	CTGACTGCTGTTGCGATTAGT	1109	LTAVAIS	2326	102.542
1051	GTCTCGGGACAAAGTGCGTAC	1110	VSGQSAY	2327	102.541
1052	GGTGAAACTAACTTCCCAACT	1111	GETNFPT	2328	102.532
1053	AATGATAATAGGTCGATGAAT	1112	NDNRSMN	2329	102.526
1054	CGATCAGGCGACCCTAAAAAC	1113	RSGDPKN	2330	102.519
1055	TGGGAGAGTGATAAGTTTCGT	1114	WESDKFR	2331	102.514
1056	CAGGTTAATCATAATACTAGT	1115	QVNHNTS	2332	102.514
1057	GGGTGGTCGAACAACGAACTA	1116	GWSNNEL	2333	102.507
1058	CGGGCTGTGCTTGCGACTAAT	1117	RAVLATN	2334	102.49
1059	CATATGGGTTTGAATGAGCTT	1118	HMGLNEL	2335	102.484
1060	GGAGAAAGCTCCTCAATAAGC	1119	GESSSIS	2336	102.477
1061	ATACACAAATCTAGCGTCGAA	1120	IHKSSVE	2337	102.473
1062	ATGTCCGGATCCATGATATCA	1121	MSGSMIS	2338	102.463
1063	TTGAGTCTGGCTGGGAATAGG	1122	LSLAGNR	2339	102.448
1064	TCTGCAACAACGAACCACGGA	1123	SATTNHG	2340	102.441
1065	TCTACGGAGTCTAATGCTAGT	1124	STESNAS	2341	102.43
1066	CCGATTGCTGAGAGGCCTTCT	1125	PIAERPS	2342	102.428
1067	TTACTTCCAAACAACACCCAC	1126	LLPNNTH	2343	102.424
1068	GGGACTCTTAAGAAGGATGCG	1127	GTLKKDA	2344	102.412
1069	GCTCTTGAGAATCGGAGTCTG	1128	ALENRSL	2345	102.408
1070	ACCACCGGGAACTCCACGATG	1129	TTGNSTM	2346	102.383
1071	GTGTATGATAGTGCGCCTAAT	1130	VYDSAPN	2347	102.366
1072	CTACTATCTAAAGGGGACTCC	1131	LLSKGDS	2348	102.346
1073	TCTTACGCCATAAACCAATCA	1132	SYAINQS	2349	102.335
1074	GGAGGAGGGGAACGTTCCACG	1133	GGGERST	2350	102.323
1075	ATTCAGGTTAGTGGTAGTCAG	1134	IQVSGSQ	2351	102.315
1076	TATCCTGTTTCGCTTTCGCCG	1135	YPVSLSP	2352	102.312
1077	GAGTTGGGTAATAAGACGGCT	1136	ELGNKTA	2353	102.311
1078	TCGGGGGTAAACTTCGGAGTA	1137	SGVNFGV	2354	102.287
1079	GCGTGGAGTTCGCCGAGTGGG	1138	AWSSPSG	2355	102.285
1080	GGTGTGAATTATCATACTACG	1139	GVNYHTT	2356	102.261
1081	CTGATTGGGGAGCTTAAGATG	1140	LIGELKM	2357	102.255
1082	TATCTGAATAGTAAGCAGCTT	1141	YLNSKQL	2358	102.212
1083	ACTGTTGATAGGCCGATTGTG	1142	TVDRPIV	2359	102.191
1084	GTCAGCAAAACCAAAGACTCG	1143	VSKTKDS	2360	102.184
1085	CAAGCTGGGAACGCGCCAAGG	1144	QAGNAPR	2361	102.1806
1086	CAAGACCAAACGAGCAACCGT	1145	QDQTSNR	2362	102.177
1087	GATACTACGTATCGGAATACT	1146	DTTYRNT	2363	102.173
1088	GGGACAACCGAAGTTAACAAA	1147	GTTEVNK	2364	102.17
1089	GGGTTTACTAATACGAGTAAG	1148	GFTNTSK	2365	102.152
1090	GTGCAGAAGAATGATGTGCTT	1149	VQKNDVL	2366	102.14
1091	AGCGTCAACAACATGCGACTC	1150	SVNNMRL	2367	102.1324
1092	TTCAGTGCCGCCTTACCGTTA	1151	FSAALPL	2368	102.13
1093	GACGTCCCAAACAACAAAAGG	1152	DVPNNKR	2369	102.126
1094	GGTGAGACTATGCGTCATAAT	1153	GETMRHN	2370	102.119
1095	ATTCGGACTTCTGTGATTAAT	1154	IRTSVIN	2371	102.103
1096	CCGCGTGCTCCTGGTCATAAT	1155	PRAPGHN	2372	102.101
1097	AGTGTTGCGCATCCTTTGTCT	1156	SVAHPLS	2373	102.101
1098	ATGACAATAACCGTCGAACCG	1157	MTITVEP	2374	102.096
1099	CCATTAAACGCGAACGGCTCC	1158	PLNANGS	2375	102.094
1100	AATAGGCAGCGGGATTTTGAG	1159	NRQRDFE	2376	102.073
1101	GATATTCATAATCCGCGTACG	1160	DIHNPRT	2377	102.073
1102	TGGATAGCAGGAAACCACTCC	1161	WIAGNHS	2378	102.07
1103	TCTACTCATCATGCTGATCGT	1162	STHHADR	2379	102.069
1104	CCGGAATCCGCCGCCAAAAGC	1163	PESAAKS	2380	102.058
1105	CACTCCGACAAAGTCTCCTCA	1164	HSDKVSS	2381	102.051
1106	TCAAACAGCGCCGACGCGGGG	1165	SNSADAG	2382	102.047
1107	GAGTTTCAGAGGATTCGTGAG	1166	EFQRIRE	2383	102.039
1108	TCCGCGGGGATGACATTGGAC	1167	SAGMTLD	2384	102.016
1109	ACTCAAACTTCTACCTGGACC	1168	TQTSTWT	2385	102.009
1110	ACGACACTAACGCAAACGGAC	1169	TTLTQTD	2386	102.003
1111	GCCTCGAAAGGCTTCGGCCAC	1170	ASKGFGH	2387	101.991
1112	CCGGCTACGATGATTAGTGAG	1171	PATMISE	2388	101.985
1113	ACTGACTCATCTGCAGACTCC	1172	TDSSADS	2389	101.981
1114	TCAACCAGAAAAGAACACGAC	1173	STRKEHD	2390	101.98
1115	GGTGATATTTCTTATAGGGTT	1174	GDISYRV	2391	101.977
1116	ATGGGGTATGTTGATAGTCTG	1175	MGYVDSL	2392	101.953
1117	CAAACCATCACCTCACAAATG	1176	QTITSQM	2393	101.941
1118	TCGATTGGGTATTCGCCTCCG	1177	SIGYSPP	2394	101.939
1119	TCATCCCCAGACTCGTACAGA	1178	SSPDSYR	2395	101.921
1120	ATTAGTCCGAGTGCTTCTAAT	1179	ISPSASN	2396	101.855
1121	TATCCGGCTGATCATCGGACT	1180	YPADHRT	2397	101.85
1122	CACACCGGCCAAACACCATCA	1181	HTGQTPS	2398	101.837
1123	CAGACGACTATTCTGGCTGCT	1182	QTTILAA	2399	101.837
1124	GATGGTACGAGGCAGGTTCAT	1183	DGTRQVH	2400	101.836
1125	AGGAGTAGTCCTGCGACGAAT	1184	RSSPATN	2401	101.829
1126	GCGATGAGTCATACGTATAAG	1185	AMSHTYK	2402	101.813
1127	ATGGCGGCTCCGCCGGAGCAT	1186	MAAPPEH	2403	101.802
1128	GGTCCTAGTACTTCGGAGGCG	1187	GPSTSEA	2404	101.794
1129	CATAATCATGATAGGTCGTCT	1188	HNHDRSS	2405	101.7829
1130	GTGGTCCCATCGACCCAAGCA	1189	WPSTQA	2406	101.781
1131	ATTCCTGTGACTACTCGTAAT	1190	IPVTTRN	2407	101.722
1132	AACCAACTCGTACGCGGGACA	1191	NQLVRGT	2408	101.717
1133	GGGTTTGCGCTTACGGGTACG	1192	GFALTGT	2409	101.696
1134	TCTAAGGGTGGTGATATGGTG	1193	SKGGDMV	2410	101.666
1135	GCTCGACCAGGCCAATCTATG	1194	ARPGQSM	2411	101.6287
1136	AAAGCAGACTACGAATCCTCC	1195	KADYESS	2412	101.626
1137	GGACCAAGTTCGCACATCGTT	1196	GPSSHIV	2413	101.616
1138	GAAGTTGTCAAAACCACGCAC	1197	EVVKTTH	2414	101.61
1139	ACTTTGGATAATAATCATTCT	1198	TLDNNHS	2415	101.604
1140	ACGATTTATAATATGGGTCCG	1199	TIYNMGP	2416	101.599
1141	TCTACCATGAACACGATCACG	1200	STMNTIT	2417	101.597
1142	ACGCTGGCGCGGACTACTGAG	1201	TLARTTE	2418	101.581
1143	TTGATTTCTTCGCAGACTTCT	1202	LISSQTS	2419	101.553
1144	CAGACTGCGTCTGGTGATACT	1203	QTASGDT	2420	101.497
1145	GCGCATGGTGCTTTTCCGGTT	1204	AHGAFPV	2421	101.495
1146	GGGGAGACGCGGTCGACTGCT	1205	GETRSTA	2422	101.494
1147	AACAACTACGCCTACTCCGCT	1206	NNYAYSA	2423	101.493
1148	GAGGCTTATCAGACTGAGAAG	1207	EAYQTEK	2424	101.49
1149	TCTCTAGCACACGCCGTAAGC	1208	SLAHAVS	2425	101.485
1150	ACGTATCAGTTGAGTGGGAAT	1209	TYQLSGN	2426	101.452
1151	ATGAGCGAAAGGTTGCGGATA	1210	MSERLRI	2427	101.431
1152	GGGTCGGGGAAAGACCCAGGG	1211	GSGKDPG	2428	101.43
1153	TACAACAGCAACGCTTCTGTA	1212	YNSNASV	2429	101.428
1154	ACGAGGGGTGATATGGAGTTT	1213	TRGDMEF	2430	101.424
1155	GGAATCACCGGAAGCCCCGGC	1214	GITGSPG	2431	101.42
1156	CAACACACCGCCCACCCCATG	1215	QHTAHPM	2432	101.416
1157	GATACGGCGAATCGTTCGACT	1216	DTANRST	2433	101.407
1158	TCGGCACACGACGCAAGACTA	1217	SAHDARL	2434	101.387
1159	CTTAATCATACTCTGGGGCAT	1218	LNHTLGH	2435	101.385
1160	GGGTTTGAGACGAGTAGTCCT	1219	GFETSSP	2436	101.369
1161	GGTACGAGTGCGGAGAGTCGG	1220	GTSAESR	2437	101.366
1162	CATGCTAATTATGTTGAGGTG	1221	HANYVEV	2438	101.345
1163	ACAACGAAACCGGTCGCGGAA	1222	TTKPVAE	2439	101.338
1164	TCGACCGCCGTTACTAACTCA	1223	STAVTNS	2440	101.304
1165	CTGGGGCTTGCTGGTCAGGTT	1224	LGLAGQV	2441	101.304
1166	GTGCTTAAGGGTACGTTTCCG	1225	VLKGTFP	2442	101.298
1167	ATGAATGAGCCTGGTAGGACG	1226	MNEPGRT	2443	101.283
1168	ACTTCTGATCCTTTGAGGAAT	1227	TSDPLRN	2444	101.252
1169	CGTGATACTAATACGGATAAG	1228	RDTNTDK	2445	101.234
1170	GAGTCTGATTTGCGTCAGCGG	1229	ESDLRQR	2446	101.225
1171	TCCGGAATGGCCGGCCTTTCC	1230	SGMAGLS	2447	101.211
1172	ATAGCAACAACGTCTGGGCGG	1231	IATTSGR	2448	101.21
1173	ACGATTAGGAGTGAGGGTTTT	1232	TIRSEGF	2449	101.202
1174	GGTCTGTCTATTACTATTGCG	1233	GLSITIA	2450	101.176
1175	CCGCCTACTAATGGGCGTATG	1234	PPTNGRM	2451	101.17
1176	CTACAAGACCGGGCAACGAAC	1235	LQDRATN	2452	101.165
1177	CTTAAATCGACCGGTGACCAC	1236	LKSTGDH	2453	101.132
1178	GATAATAATAATCAGGTTTAT	1237	DNNNQVY	2454	101.13
1179	GTGCATATGGAGTCGTATGCG	1238	VHMESYA	2455	101.111
1180	GACCAAATAGGGCACGGAACA	1239	DQIGHGT	2456	101.106
1181	GGGACGGGGCCGCATGGTACT	1240	GTGPHGT	2457	101.0712
1182	ATTGGGAATAATACTGGTCTT	1241	IGNNTGL	2458	101.0529
1183	TTAAACGCAGAATACACCAAC	1242	LNAEYTN	2459	101.047
1184	GTGACGTCGTCTGCTAGTGGT	1243	VTSSASG	2460	101.027
1185	ACGCATGTTGCTAAGCCTGAT	1244	THVAKPD	2461	101.017
1186	CCGATGAACAAAGACATACTG	1245	PMNKDIL	2462	100.9906
1187	CTTAGTTTGAATATGAATGAG	1246	LSLNMNE	2463	100.99
1188	GTCGGCAACTCAAGCACTCAC	1247	VGNSSTH	2464	100.99
1189	GGCCACGGAAGTGACTTGACC	1248	GHGSDLT	2465	100.9576
1190	CTTACACAAAACCCAACGAAC	1249	LTQNPTN	2466	100.934
1191	CCGAGTGATCATATGCGGACT	1250	PSDHMRT	2467	100.8849
1192	CCTGATAGTCGTTTGGCGGCT	1251	PDSRLAA	2468	100.843
1193	TGGGGTAGTGAGGGGACGATT	1252	WGSEGTI	2469	100.84
1194	AAACCGACAAACGACTCGTAC	1253	KPTNDSY	2470	100.821
1195	AACCGCGGAACAGAAGTTTAC	1254	NRGTEVY	2471	100.8147
1196	CACGTGATCACAACAAAAGAC	1255	HVITTKD	2472	100.7896
1197	ATTGTGTCTAATCCGCCGGCG	1256	IVSNPPA	2473	100.76
1198	ATGCGTAACGACCAACAACTT	1257	MRNDQQL	2474	100.7503
1199	TTTCAGCGTGATGTTGGTCAT	1258	FQRDVGH	2475	100.7392
1200	GCCAACGACAACACCAAACAA	1259	ANDNTKQ	2476	100.7364
1201	TCTGTTCCGCATGCGGGGGAT	1260	SVPHAGD	2477	100.7276
1202	AATGCTACTCCGCCGAATCAT	1261	NATPPNH	2478	100.6678
1203	TCAGAACACACATCAGTTCTA	1262	SEHTSVL	2479	100.64
1204	GCCATGTCCCAAACGGACATC	1263	AMSQTDI	2480	100.628
1205	CCTAAGGCTCCGCTTAATAAT	1264	PKAPLNN	2481	100.627
1206	ACCAACAACTTACTCGCACAA	1265	TNNLLAQ	2482	100.55
1207	CAGCGTCAGGGTTCGGGGGTT	1266	QRQGSGV	2483	100.5318
1208	CGCAGTGACACCACTAACGCC	1267	RSDTTNA	2484	100.51
1209	GAGGCTGATAAGAATGGTGTT	1268	EADKNGV	2485	100.386
1210	ATGCTGGGGGGTTTTGCGCAG	1269	MLGGFAQ	2486	100.3622
1211	ATGACACACCTCAGCACAGAC	1270	MTHLSTD	2487	100.267
1212	GTTTTGTCTGATAAGGCGTTT	1271	VLSDKAF	2488	100.231
1213	ACACCCTCCGGTACCATAAAA	1272	TPSGTIK	2489	100.22
1214	ATTATTCTTATGGGTCAGAGT	1273	IILMGQS	2490	100.213
1215	CTTTCGGGGGGTGAGACTCTT	1274	LSGGETL	2491	100.154
1216	ACCGACGGCGCCCTGGGTTAC	1275	TDGALGY	2492	100.129
1217	GGGAATAAGGCTGCGCTGACG	1276	GNKAALT	2493	100.066