CANCER STEM CELL-SPECIFIC MOLECULE

[0001] This is a continuation of U.S. application Ser. No. 14/354,517, filed Apr. 25, 2014, which is the U.S. national stage of PCT Application No. PCT/JP2012/077714, filed Oct. 26, 2012, which claims the benefit of Japanese Patent Application No. 2011-237438, filed Oct. 28, 2011, and Japanese Patent Application No. 2012-091142, filed Apr. 12, 2012.

[0002] The Sequence Listing is submitted as an ASCII text file, created on Feb. 10, 2016, 30.4 KB, which is incorporated by reference herein.

[0003] The present invention relates to cell surface molecules specific to Lgr5-positive cancer stem cells with a high proliferative potential or Lgr5-negative cancer stem cells with a low proliferative potential; and pharmaceutical compositions that comprise as an active ingredient an antibody against such a cell surface molecule. The present invention also relates to reagents for detecting cancer stem cells and methods for selecting cancer patients, which use an antibody described above.

[0004] Cancer stem cells (CSCs) are considered to be the origins of cancer. The reason is that these cells have the ability to self-renew and differentiate to form a tumor hierarchy (Non-patent Document 1). Furthermore, CSCs can migrate and be tolerant to anti-cancer drug therapy (Non-patent Document 1). Since CSCs are believed to be a rare subset in tumors, there have been numerous efforts to characterize them based on cell surface markers and tumor-initiating activity in xenograft transplantations. CSCs have been reported in several types of cancer, including acute myelocytic leukemia (AML) (Non-patent Documents 2 and 3), breast cancer (Non-patent Document 4), glioma (Non-patent Document 5), head and neck cancer (Non-patent Document 6), pancreatic cancer (Non-patent Documents 7 and 8), lung cancer (Non-patent Document 9), prostatic cancer (Non-patent Documents 10 and 11), mesenchymal neoplasm (Non-patent Document 12), and melanoma (Non-patent Documents 13 and 14). Earlier studies of O'Brien et al. (Non-patent Document 15) and Ricci-Vitiani et al. (Non-patent Document 16) reported that CD133 served as a CSC marker for colorectal cancer. Thereafter, different research groups have reported other markers: CD44, EpCAM, CD166 (Non-patent Document 17), and ALDH (Non-patent Documents 18 and 19). Recently, Pang et al. demonstrated that CD26 serves as a marker for a CSC subpopulation with metastatic capacity (Non-patent Document 20).

[0005] To isolate CSCs, most studies have employed a cell selection approach using in combination CSC markers such as EpCAM^high/CD44⁺/CD166⁺ (Non-patent Document 17), CD133⁺/CD44⁺ (Non-patent Document 21), CD44^high/ALDH⁺ (Non-patent Document 18), and ALDH1⁺/CD133⁺ (Non-patent Document 19). In vitro spheroid (cell mass) cultures and direct cancer cell xenograft transplantation to immunodeficient mice have also been used to enrich CSCs (Non-patent Document 22). However, there was a necessity to prepare a large number of cancer stem cells with a high purity for further understanding the properties of CSCs.

[0006] One challenge in isolating CSCs arises from the phenotypic heterogeneity and/or instability of these cells (Non-patent Document 29). Three-dimensional spheroid cultures are often used as a CSC source. Spheroid cultures are applicable directly to tumor cells of clinically resected specimens and enable maintenance of heterogeneous CSC populations, and can have certain potential advantages compared to xenograft transplantations. Due to the heterogeneity, however, results of biochemical analyses often show complicated CSC characteristics. CSC selection using antibodies against cell surface marker proteins is commonly used to isolate CSCs, but the number and purity of cells obtained by this method is limited. On the other hand, phenotypes from xenografts remain stable even after frequent passages, and using xenografts as a source of CSCs is also a common approach. However, there is an argument that xenograft passages in mice only select cells viable in mice and result in elimination of cells that are hardly affected by such an environment. It goes without saying that CSCs in xenograft tumors reflect the characteristics of original CSCs, as long as they maintain the self-renewability and lineage differentiation capacity of the original tumor.

[0007] Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) was originally identified as an orphan G-protein-coupled receptor of the glycoprotein hormone receptor family (Non-patent Documents 23 and 24) and was demonstrated to be a Wnt target gene whose expression is restricted to the crypt (Non-patent Document 25). The discovery that Lgr5-positive columnar cells can regenerate all epithelial lineages (Non-patent Document 25) and that a single Lgr5-positive cell can form crypt-villus organoids in vitro without a mesenchymal niche (Non-patent Document 26), conclusively proves that Lgr5-positive cells are stem cells in the normal large intestine. It has also been reported that Lgr5-positive cells form adenomas in the absence of Apc (Non-patent Document 27) and Lgr5 is expressed in colorectal cancer cell lines (Non-patent Document 25). When considered together, the findings described above suggest that Lgr5-positive cells are an origin of colorectal cancer (Non-patent Document 25). It has been proven that, as in stem cells of the normal large intestine, Wnt activity is essential for in vitro and in vivo proliferation of CSCs and that exogenous HGF enhances Wnt activity (Non-patent Document 28).

[0008] Lgr5 was identified as a marker for normal large intestine stem cells, and has been demonstrated to serve as a marker for origins of colorectal cancer (Patent Document 1 and Non-patent Document 30). Furthermore, Lgr5 was reported to be a protein that is over-expressed in colorectal cancer stem cells (Patent Document 2). The biological role of Lgr5 in the development of large intestine cancer remains poorly understood.

[0009] To date, various anti-cancer drugs and cancer therapeutic methods have been developed, but there are still issues to be solved, such as poor effectiveness, adverse effects, or being effective in only a limited number of patients. In recent years, therapeutic methods for targeting cancer stem cells have drawn attention, but their effectiveness and adverse effects remain poorly understood (Non-patent Document 31).

• [0010] Patent Document 1: US20100275280
• [0011] Patent Document 2: WO09/005809

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• [0023] Non-patent Document 12: Wu C, et al. (2007) Side population cells isolated from mesenchymal neoplasms have tumor initiating potential. Cancer Res 1:8216-8222.
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[0043] The present invention was achieved in view of the circumstances described above. The present invention isolates two types of cancer stem cell populations, which are substantially homogeneous and characterized with Lgr5, a cell surface marker, and identifies cell membrane molecules expressed specifically in the cancer stem cells. An objective of the present invention is to provide agents for treating cancer which use antibodies to those cell membrane molecules. Another objective of the present invention is to provide reagents for detecting cancer stem cells, methods for diagnosing and selecting cancer patients, which use antibodies to cell membrane molecules expressed specifically in cancer stem cells.

[0044] In order to prove the stem cell theory that can explain the mechanism of oncogenesis, maximum efforts have been made to identify, isolate, and characterize cancer stem cells (CSCs). However, obtaining highly pure CSCs in an amount sufficient to characterize them remained difficult. It was therefore also difficult to identify cell membrane molecules specifically expressed on cancer stem cells. The present inventors conducted dedicated studies to achieve the above-described objectives.

[0045] To isolate large intestine CSCs from human colorectal cancer xenografts maintained in NOG mice, the present inventors established, for the first time, a method for preparing a large quantity of highly pure large intestine CSCs using an in vitro monolayer culture (also simply referred to as adherent culture) with serum-free stem cell media. Specifically, the present inventors demonstrated that a large number of large intestine CSCs can be obtained with high purity by performing an adherent culture of isolated cells derived from moderately-differentiated human colorectal cancer xenografts maintained in NOD/Shi-scid, IL-2Rγ null (NOG) mice. Under these conditions, only large intestine CSCs can grow, survive, and expand, and thus, the present inventors were able to obtain substantially homogeneous large intestine CSCs with high purity. When passaged by adherent culture using serum-free stem cell media, large intestine CSCs prepared by the above method were maintained stably without phenotypic alterations over a month or more. The cells expressed various previously-reported colorectal cancer stem cell markers (CD133, CD44, EpCAM, CD166, CD24, CD26, and CD29), and exhibited tumor-initiating activity at a rate of almost 100%. Furthermore, the cells formed tumors having the same histopathological features (hierarchical organization) as the original primary tumor. The cells are also characterized by being highly proliferative under adherent culture conditions and positive for Lgr5, a cell surface marker. Furthermore, the high proliferative Lgr5-positive cancer stem cells, when administered via the caudal vein to mice, formed tumor masses in organs such as lung and liver, which indicates that the cells play an essential role in cancer metastasis.

[0046] On the other hand, low proliferative Lgr5-negative cancer stem cells were isolated by treating with anti-cancer agents such as irinotecan and 5-FU cancer stem cells that are positive for cell surface marker Lgr5 and highly proliferative under adherent culture conditions. Furthermore, the isolated low proliferative Lgr5-negative cancer stem cells were revealed to be converted to high proliferative Lgr5-positive cancer stem cells by re-culturing under adherent culture conditions. This demonstrates that high proliferative Lgr5-positive cancer stem cells and low proliferative Lgr5-negative cancer stem cells are interconvertible to each other and thus have an intrinsic interchangeability. Due to this ability, actively proliferating Lgr5-positive large intestine CSCs, when cultured under an altered culture condition or in the presence of an anti-cancer drug, were converted into the Lgr5-negative quiescent state. Alternatively, Lgr5-negative CSCs, when isolated and then cultured again under an adherent culture condition, were converted to Lgr5-positive CSCs that proliferate actively. These cells also exhibited tumor-initiating activity at a rate of almost 100%. Furthermore, the high proliferative Lgr5-positive cancer stem cells, when inoculated via the caudal vein to mice, formed tumor masses in organs such as lung and liver, which indicates that the cells play an essential role in cancer metastasis.

[0047] The interconversion of CSCs between the two types of conditions due to environmental changes may be helpful in explaining drug resistance and recurrence of cancer. The involvement of Lgr5-negative CSCs in oncogenesis can be correlated with the basic nature of stem cells. A plausible hypothesis is that CSCs can use intrinsic means to convert into a different subset of a cell population under environmental changes such as by aggressive treatment with anti-cancer agents. CSCs, when exposed to stress such as anti-cancer drugs or changes in the culturing environment, convert themselves to low proliferative Lgr5-negative CSCs in order to survive avoiding the stress. Once the stress is removed, the cells can change again into high proliferative Lgr5-positive CSCs and start to proliferate. This implies that CSCs have a self-defense ability based on an intrinsic mechanism to adapt to new environments (FIG. 36).

[0048] The above-described research findings by the present inventors demonstrate that high proliferative Lgr5-positive and low proliferative Lgr5-negative cancer stem cells both play important roles in cancer development, formation, metastasis, recurrence, drug resistance, etc., and can be major target cells in the development of anti-cancer agents. In particular, high proliferative Lgr5-positive cancer stem cells are considered to be involved in oncogenesis and metastasis while low proliferative Lgr5-negative cancer stem cells are thought to be involved in cancer recurrence. Thus, if cell surface molecules that are expressed specifically on high proliferative Lgr5-positive cancer stem cells and low proliferative Lgr5-negative cancer stem cells can be identified, such molecules would enable therapies using antibodies and discovery of new anti-cancer agents and reagents for detecting cancer stem cells.

[0049] Specifically, the present invention provides:

[1] a pharmaceutical composition comprising as an active ingredient at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8;
[2] the pharmaceutical composition of [1], which is an anti-cancer agent;
[3] the pharmaceutical composition of [2], which is an agent for inhibiting cancer recurrence;
[4] the pharmaceutical composition of [2], which is an agent for inhibiting cancer metastasis or an agent for postoperative adjuvant therapy;
[5] the pharmaceutical composition of [4], which is an agent for inhibiting cancer metastasis or an agent for postoperative adjuvant therapy against Lgr5-positive cancer, which comprises as an active ingredient at least one antibody that binds to a protein of SEQ ID NOs: 1 to 6;
[6] the pharmaceutical composition of [2], which is a therapeutic agent against drug-resistant cancer;
[7] the pharmaceutical composition of [6], which is a therapeutic agent against Lgr5-negative cancer, and which comprises as an active ingredient at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8;
[8] the pharmaceutical composition of [7] wherein the Lgr5-negative cancer is a drug-resistant cancer;
[9] the pharmaceutical composition of any one of [2] to [8], which is an agent for inhibiting cancer stem cell proliferation or an agent for disrupting cancer stem cells;
[10] the pharmaceutical composition of any one of [2] to [9], wherein the cancer is a solid cancer;
[11] the pharmaceutical composition of any one of [2] to [10], wherein the cancer is a digestive system cancer;
[12] the pharmaceutical composition of any one of [2] to [11], wherein the cancer is a colorectal cancer;
[13] the pharmaceutical composition of any one of [1] to [12], wherein the antibody is a monoclonal antibody;
[14] the pharmaceutical composition of any one of [1] to [13], wherein the antibody is a chimeric antibody, a humanized antibody, or a human antibody;
[15] the pharmaceutical composition of any one of [1] to [14], wherein the antibody is an antibody fragment;
[16] the pharmaceutical composition of [15], wherein the antibody is linked to a cytotoxic substance or a proliferation inhibitor;
[17] the pharmaceutical composition of any one of [1] to [14], wherein the antibody has a cytotoxic activity;
[18] the pharmaceutical composition of [17], wherein the cytotoxic activity is ADCC;
[19] the pharmaceutical composition of [17] or [18], which comprises an antibody with modified sugar chains whose sugar chain composition has been altered to increase the ratio of defucosylated antibody or to increase the ratio of antibody attached with bisecting N-acetylglucosamine;
[20] the pharmaceutical composition of [17], wherein the cytotoxic activity is CDC;
[21] the pharmaceutical composition of any one of [1] to [20], wherein the antibody has a neutralizing activity;
[22] the pharmaceutical composition of any one of [2] to [21], or a pharmaceutical composition comprising the polypeptide of SEQ ID NO: 9 or a polypeptide resulting from addition, deletion, and/or substitution of one or several amino acids in the polypeptide of SEQ ID NO: 9, which is to be used in combination with a chemotherapeutic agent simultaneously or after chemotherapeutic treatment;
[23] a reagent for detecting a cancer stem cell, which comprises as an active ingredient at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8;
[24] the reagent of [23], wherein it is used in detecting an Lgr5-positive cancer stem cell, and wherein the antibody is at least one antibody that binds to a protein of SEQ ID NOs: 1 to 6;
[25] the reagent of [23], wherein it is used in detecting an Lgr5-negative cancer stem cell, and wherein the antibody is at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8;
[26] a method for diagnosing cancer or selecting a cancer patient (a method for testing and/or selecting cancer), wherein the method comprises, by using at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8, detecting the presence of at least one of the proteins in a sample isolated from a cancer patient;
[27] the method of [26], wherein it is used in diagnosing Lgr5-positive cancer or selecting a cancer patient, and wherein the antibody is at least one antibody that binds to a protein of SEQ ID NOs: 1 to 6;
[28] the method of [26], wherein it is used in diagnosing Lgr5-negative cancer or selecting a cancer patient, and wherein the antibody is at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8;
[29] a method for assessing the effectiveness of the pharmaceutical composition of any one of [1] to [22], wherein the method comprises detecting the presence of one or more of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins in a sample isolated from a subject administered with the pharmaceutical composition;
[30] the method of [29], which uses at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8; and
[31] the method of [29], which uses polynucleotides encoding the proteins of SEQ ID NOs: 1 to 8 and/or complementary strands thereof.

[0050] The present invention also provides:

[A1] a method for treating cancer, comprising administering to a subject at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8;
[A2] at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8 for use in the treatment of cancer;
[A3] use of at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8 for producing an anti-cancer agent; and
[A4] a process for manufacturing an anti-cancer agent, which comprises the step of using at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8.

[0051] In a non-limiting embodiment of the present invention, treatment of cancer includes inhibition of cancer recurrence, inhibition of cancer metastasis, postoperative adjuvant therapy, treatment of drug-resistant cancer, inhibition of cancer stem cell proliferation, and disruption of cancer stem cells; and anti-cancer agents include agents for inhibiting cancer recurrence, agents for inhibiting cancer metastasis, agents for postoperative adjuvant therapy, agents for treating drug-resistant cancer, agents for inhibiting cancer stem cell proliferation, and agents for disrupting cancer stem cells.

[0052] Furthermore, the present invention provides:

[B1] a reagent for detecting the presence of one or more of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins, preferably, a reagent for detecting a cancer stem cell, a reagent for cancer diagnosis, a reagent for selecting a cancer patient, or a reagent for testing the effectiveness of the pharmaceutical compositions of [1] to [22], which contains at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8, or a portion of a polynucleotide encoding the protein of SEQ ID NOs: 1 to 8 and/or the complementary strand thereof;
[B2] a method for detecting a cancer stem cell, diagnosing cancer, selecting a cancer patient, or testing the effectiveness of the pharmaceutical compositions of [1] to [22], which comprises detecting the presence of one or more of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins in a sample isolated from a cancer patient using, preferably, at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8, or a portion of a polynucleotide encoding the protein of SEQ ID NOs: 1 to 8 and/or the complementary strand thereof;
[B3] a reagent for detecting the presence of one or more of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins, preferably at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8, or a portion of a polynucleotide encoding the protein of SEQ ID NOs: 1 to 8 and/or the complementary strand thereof, which is for use in detecting a cancer stem cell, diagnosing cancer, selecting a cancer patient, or testing the effectiveness of pharmaceutical compositions of [1] to [22];
[B4] use of a reagent for detecting the presence of one or more of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins, preferably at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8, or a portion of a polynucleotide encoding the protein of SEQ ID NOs: 1 to 8 and/or the complementary strand thereof, which is for producing a reagent for detecting a cancer stem cell, a reagent for diagnosing cancer, a reagent for selecting a cancer patient, or a reagent for testing the effectiveness of the pharmaceutical compositions of [1] to [22]; and
[B5] a process for producing a reagent for detecting a cancer stem cell, a reagent for diagnosing cancer, a reagent for selecting a cancer patient, or a reagent for testing the effectiveness of the pharmaceutical compositions of [1] to [22], which comprises using a reagent for detecting the presence of one or more of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins, preferably at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8, or a portion of a polynucleotide encoding the protein of SEQ ID NOs: 1 to 8 and/or the complementary strand thereof.

[0126] The present invention relates to cell surface molecules that are specifically expressed on cancer stem cells, and pharmaceutical compositions (anti-cancer agents, etc.) and reagents for detecting cancer stem cells, which use antibodies against the cell surface molecules.

[0127] Herein, “cancer” refers to the physiological condition in mammals, which is typically characterized by unregulated cell growth, or such a physiological condition. Herein, cancer types are not particularly limited, and include those listed below. Carcinomas (epithelial cancers) include pancreatic cancer, prostatic cancer, breast cancer, skin cancer, cancers of the digestive tract, lung cancer, hepatocellular carcinoma, cervical cancer, uterine cancer, ovary cancer, fallopian tube cancer, vaginal cancer, liver cancer, bile duct cancer, bladder cancer, ureter cancer, thyroid cancer, adrenal cancer, kidney cancer, and cancers of other glandular tissues. Sarcomas (non-epithelial tumors) include liposarcoma, leiomyosarcoma, rhabdomyosarcoma, synovial sarcoma, angiosarcoma, fibrosarcoma, malignant peripheral nerve sheath tumor, gastrointestinal stromal tumor, desmoid tumor, Ewing's sarcoma, osteosarcoma, chondrosarcoma, leukemia, lymphoma, myeloma, tumors of other parenchymal organs, for example, melanoma and brain tumor (Kumar V, Abbas A K, Fausio N. Robbins and Cotran Pathologic Basis of Disease. 7th Ed. Unit I: General Pathology, 7: Neoplasia, Biology of tumor growth: Benign and malignant neoplasms. 269-342, 2005).

[0128] Herein, “tumor” refers to arbitrary benign (non-cancerous) and malignant (cancerous) tissue masses, including pre-cancerous lesions, which result from overgrowth or overexpansion of cells.

[0129] Herein, cancer stem cell (CSC) refers to cells having the abilities described in (i) and/or (ii) below.

(i) The ability to self-renew. The self-renewal ability refers to the ability of either or both of the divided daughter cells to produce cells which maintain the same capacity and the degree of differentiation as the parental cell in terms of cell lineage.
(ii) The ability to differentiate into various types of cancer cells that constitute a cancer cell mass. Like normal stem cells, various types of cancer cells differentiated from cancer stem cells generate a hierarchical organization with cancer stem cells at the top in terms of cell lineage. Various types of cancer cells are generated in a sequential manner from cancer stem cells. This results in the formation of a cancer cell mass that exhibits a variety of features.

[0130] Cancer stem cell refers to a cancer cell that has the ability to form cancers as well as, like normal stem cell, pluripotency and self-renewal ability. Cancer stem cells generate a hierarchical organization with cancer stem cells at the top. Various types of cancer cells are generated in a sequential manner from cancer stem cells. This results in the formation of a cancer cell mass that exhibits a variety of features. Cancer cell mass refers to, not a group of individual cells, but a mass formed by the adhesion of cells etc. as in human tumor tissue, which is built with cancer cells, and other cells such as stromal cells and blood cells, extracellular matrix such as collagen and laminin, and so on.

[0131] The origin of cancer stem cells, which are the target in therapy using pharmaceutical compositions of the present invention, is not particularly limited; it is possible to use cancer stem cells derived from mammals such as humans, monkeys, chimpanzees, dogs, bovines, pigs, rabbits, rats, and mice. However, cancer stem cells derived from humans are preferred, and those derived from human tumor tissues are more preferred.

[0132] Cancer stem cells to be detected using the present invention are preferably those which reconstitute the hierarchical structure of cancer tissues. For example, cancer cell lines are prepared by grafting cancer tissues from which the detected cancer stem cells have been collected, into, preferably, nonhuman animals, and passaging them in such animals, and one can test whether the established cancer cell lines reconstitute the hierarchical structure of the cancer tissues. One can test whether the hierarchical structure of cancer tissues is reproduced by NOG-established cancer cell lines prepared by grafting and passaging cancer tissues in nonhuman animals, more preferably immunodeficient animals, and still more preferably NOG mice which lack functional T cells, B cells, and natural killer cells.

[0133] Alternatively, cancer stem cells to be detected using the present invention can be a spheroid (cell mass) formed by spheroid culture. “Spheroid culture” means that cancer stem cells are inoculated in a culture vessel such as non-adherent or low-adherent cell culture flasks, plates, or dishes using a medium capable of culturing cancer stem cells, and then the cells are cultured under a three-dimensionally floating condition. A cell mass formed by this method is called “spheroid”.

[0134] NOG-established cancer cell lines can be generated by a method known to those skilled in the art, for example, the method described in Fujii E. et al., Pathol Int. 2008; 58: 559-567. Human colorectal cancer, stomach cancer, lung cancer, breast cancer, pancreatic cancer, or the like is resected surgically. After mechanically mincing it with scissors, the cancer is grafted subcutaneously in NOG mice and passaged to establish cell lines. Even after passages, NOG-established cancer cell lines maintain the properties of the original human cancer tissues.

[0135] In the present invention, cancer stem cells can be selected by using cell markers. Cell markers used in the present invention include, for example, leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5), CD133, CD44, EpCAM, CD166, CD24, CD26, and CD29.

[0136] The present invention relates to molecules expressed in cancer stem cells that are positive for the expression of the cell marker Lgr5, and are adherent and highly proliferative under serum-free culture conditions. Hereinafter, such cancer stem cells are also referred to as “high proliferative Lgr5-positive cancer stem cells”.

[0137] The present invention also relates to molecules expressed in cancer stem cells that are negative for the expression of the cell marker Lgr5, and non-adherent and poorly proliferative under serum-free culture conditions. Hereinafter, such cancer stem cells are also referred to as “low proliferative Lgr5-negative cancer stem cells”.

[0138] Any culture media or liquids can be used to culture cancer stem cells of the present invention as long as they are serum-free media and capable of culturing cancer stem cells. There is no particular limitation on the culture media or liquids. For example, it is possible to use conventional basal media or mixtures thereof that are supplemented with EGF, bFGF, hLIF, HGF, NGF, NSF-1, TGFβ, TNFα, heparin, BSA, insulin, transferrin, putrescine, selenite, progesterone, hydrocortisone, D-(+)-glucose, sodium bicarbonate, HEPES, L-glutamine, or N-acetylcysteine. The concentration of EGF is not particularly limited; however, it ranges from 0.1 to 100 ng/ml, preferably from 0.5 to 50 ng/ml, and more preferably from 1 to 20 ng/ml. The concentration of bFGF is not particularly limited; however, it ranges from 0.1 to 100 ng/ml, preferably from 0.5 to 50 ng/ml, and more preferably from 1 to 20 ng/ml. The concentration of hLIF is not particularly limited; however, it ranges from 0.1 to 100 ng/ml, preferably from 0.5 to 50 ng/ml, and more preferably from 1 to 20 ng/ml. The concentration of HGF is not particularly limited; however, it ranges from 0.1 to 100 ng/ml, preferably from 1 to 50 ng/ml. The concentration of NGF is not particularly limited; however, it ranges from 0.1 to 100 ng/ml, preferably from 1 to 50 ng/ml. The concentration of NSF-1 is not particularly limited; however, it ranges from 0.1 to 100 ng/ml, preferably from 1 to 50 ng/ml. The concentration of TGFβ is not particularly limited; however, it ranges from 0.1 to 100 ng/ml, preferably from 1 to 50 ng/ml. The concentration of TNFα is not particularly limited; however, it ranges from 0.1 to 100 ng/ml, preferably from 1 to 50 ng/ml. The concentration of heparin is not particularly limited; however, it ranges from 10 ng/ml to 10 μg/ml, preferably from 2 to 5 μg/ml. The concentration of BSA is not particularly limited; however, it ranges from 0.1 to 10 mg/ml, preferably from 1 to 8 mg/ml. The concentration of insulin is not particularly limited; however, it ranges from 1 to 100 μg/ml, preferably from 10 to 50 μg/ml. The concentration of transferrin is not particularly limited; however, it ranges from 10 to 500 μg/ml, preferably from 50 to 200 μg/ml. The concentration of putrescine is not particularly limited; however, it ranges from 1 to 50 μg/ml, preferably from 10 to 20 μg/ml. The concentration of selenite is not particularly limited; however, it ranges from 1 to 50 nM, preferably from 20 to 40 nM. The concentration of progesterone is not particularly limited; however, it ranges from 1 to 50 nM, preferably from 10 to 30 nM. The concentration of hydrocortisone is not particularly limited; however, it ranges from 10 ng/ml to 10 μg/ml, preferably from 100 ng/ml to 1 μg/ml. The concentration of D-(+)-glucose is not particularly limited; however, it ranges from 1 to 20 mg/ml, preferably from 5 to 10 mg/ml. The concentration of sodium bicarbonate is not particularly limited; however, it ranges from 0.1 to 5 mg/ml, preferably from 0.5 to 2 mg/ml. The concentration of HEPES is not particularly limited; however, it ranges from 0.1 to 50 mM, preferably from 1 to 20 mM. The concentration of L-glutamine is not particularly limited; however, it ranges from 0.1 to 10 mM, preferably from 1 to 5 mM. The concentration of N-acetylcysteine is not particularly limited; however, it ranges from 1 to 200 μg/ml, preferably from 10 to 100 μg/ml. Known basal culture liquids, which are not particularly limited as long as they are suitable for culturing cancer cells from which cancer stem cells are derived, include, for example, DMEM/F12, DMEM, F10, F12, IMDM, EMEM, RPMI-1640, MEM, BME, Mocoy's 5A, and MCDB131. Of them, DMEM/F12 is preferred.

[0139] The most preferred stem cell media include DMEM/F12 medium supplemented with 20 ng/ml human EGF, 10 ng/ml human bFGF, 4 μg/ml heparin, 4 mg/ml BSA, 25 μg/ml human insulin, and 2.9 mg/ml glucose where each concentration a final concentration.

[0140] As described herein, high proliferative Lgr5-positive cancer stem cells have the properties of mesenchymal cells. Meanwhile, as described herein, low proliferative Lgr5-negative cancer stem cells have the properties of epithelial cells. Herein, “epithelial cells” refers to cells that constitute epithelial tissues in the living body.

[0141] In the present invention, the origin of cancer stem cells is not particularly limited; however, they are preferably derived from a solid cancer, more preferably a gastrointestinal cancer. Gastrointestinal cancers include, for example, esophageal cancer, stomach cancer, duodenal cancer, pancreatic cancer, bile duct cancer, gallbladder cancer, biliary tract cancer, colorectal cancer, colon cancer, and rectal cancer. A preferred gastrointestinal cancer is colorectal cancer.

[0142] Furthermore, in the present invention, cancer stem cells are preferably positive for one or more of the cell markers CD133, CD44, EpCAM, CD166, CD24, CD26, and CD29, more preferably positive for CD133, CD44, EpCAM, CD166, CD24, CD26, and CD29.

[0143] In addition, in the present invention, acetaldehyde dehydrogenase (ALDH) activity can be used as a cell marker. In the present invention, Lgr5-positive adherent cancer stem cells are positive for the ALDH activity cell marker, whereas Lgr5-negative cancer stem cells are negative for ALDH activity.

[0144] In the present invention, one or more of HLA-DMA, TMEM173, ZMAT3, and GPR110 can also be used as cell markers. Lgr5-positive adherent cancer stem cells are negative for any of the cell markers HLA-DMA, TMEM173, ZMAT3, and GPR110, while Lgr5-negative cancer stem cells are positive for any of the cell markers HLA-DMA, TMEM173, ZMAT3, and GPR110.

[0145] In the present invention, cancer stem cells preferably have the feature of reconstituting the hierarchical structure of cancer tissues.

[0146] Herein, “hierarchical structure” means that some of the unique and characteristic structures observed in a normal tissue are detected histopathologically in the structure of a tumor originated from the tissue. In general, highly-differentiated cancers reconstitute the hierarchical structure to a high degree. For example, lumen formation and mucous cells are observed in the case of tumors of glandular lumen-forming organs (stomach cancer, colorectal cancer, pancreatic cancer, liver cancer, bile duct cancer, breast cancer, lung adenocarcinoma, prostatic cancer, etc.). In the case of tumors that form squamous epithelial structures (squamous cell carcinoma of lung, skin, vaginal mucosa, etc.), layer structure formation, the tendency to keratosis, and such are observed in the epithelium. On the other hand, poorly-differentiated cancers insufficiently reconstitute the hierarchical structure, and they are said to be highly atypical (Kumar V, Abbas A K, Fausio N. Robbins and Cotran Pathologic Basis of Disease. 7th Ed. Unit I: General Pathology, 7: Neoplasia, Biology of tumor growth: Benign and malignant neoplasms. 272-281, 2005). Since the hierarchical structure is considered to be reconstituted as a result of various biological reactions, cancer stem cells that reconstitute it are thought to be highly useful.

[0147] “Reconstitution of the hierarchical structure” means that the unique and characteristic structure possessed by the original cancer stem cells is also observed even after isolation or induction of cancer stem cells.

[0148] Furthermore, in the present invention, cancer stem cells preferably have the ability of epithelial-mesenchymal transition (EMT). Herein, the ability of epithelial-mesenchymal transition means both that epithelial cells transition into mesenchymal cells by obtaining their characteristics, and that mesenchymal cells transition into epithelial cells by obtaining their characteristics. EMT does not occur in normal cells except during the process of embryogenesis. Epithelial cells, which are bound together tightly and exhibit polarity, change into mesenchymal cells that are bound together more loosely, exhibit a loss of polarity, and have the ability to move. These mesenchymal cells can spread into tissues around the primary tumor, and also separate from the tumor, invade blood and lymph vessels, and move to new locations where they divide and form additional tumors. Drug resistance, metastasis, or recurrence of cancer can be explained by such additional tumor formation.

[0149] Furthermore, the present invention provides pharmaceutical compositions comprising as an active ingredient an antibody that binds to a molecule expressed in a substantially homogeneous cancer stem cell population comprising the above cancer stem cells of the present invention. “Substantially homogeneous” means that, when immunodeficient animals are grafted with 1000 cells, 100 cells, or 10 cells and analyzed for the frequency of formation of cancer cell populations using Extreme Limiting Dilution Analysis (Hu Y & Smyth G K., J Immunol Methods. 2009 Aug. 15; 347(1-2): 70-8) utilizing, for example, the method described in Hu Y & Smyth G K., J Immunol Methods. 2009 Aug. 15; 347 (1-2):70-8 or Ishizawa K & Rasheed Z A. et al., Cell Stem Cell. 2010 Sep. 3; 7(3):279-82, the frequency of cancer stem cells is 1/20 or more, preferably 1/10 or more, more preferably 1/5 or more, even more preferably 1/3 or more, still more preferably 1/2 or more, and yet more preferably 1/1.

[0150] In the present invention, cancer stem cell populations can be prepared, for example, by culturing cells or a group of cells containing the cancer stem cells described herein.

[0151] Herein, “adherent culture” means that, after seeding cells into culture vessels for adherent culture, the adhered cells are cultured and passaged while non-adherent cells are removed. The cells grown to confluency are detached with Accutase and passaged into fresh adherent culture flasks, adherent culture plates, or adherent culture dishes for further culture. Culture vessels for adherent culture are not particularly limited as long as they are used for adherent culture. It is possible to appropriately select and use flasks for adherent culture or highly adherent flasks, plates for adherent culture or highly adherent plates, flat-bottomed plates for adherent culture or highly adherent flat-bottomed plates, dishes for adherent culture or highly adherent dishes, etc.

[0152] Media used for adherent culture are not particularly limited; however, it is preferable to use serum-free stem cell culture media.

[0153] Herein, “adherent” refers to the property of cells to adhere to culture vessels for adherent culture when they are cultured in the vessels.

[0154] Herein, “suspension culture” means that, after seeding cells into culture vessels for suspension culture, the floating cells are cultured and passaged while adherent cells are removed. The cells grown to confluency are passaged into fresh low adherent cell culture flasks, ultra-low adherent cell culture flasks, low adherent plates, ultra-low adherent plates, low adherent dishes, or ultra-low adherent dishes for further culture. Culture vessels for suspension culture are not particularly limited as long as they are used for suspension culture. It is possible to appropriately select and use low adherent cell culture flasks, ultra-low adherent cell culture flasks, low adherent plates, ultra-low adherent plates, low adherent dishes, ultra-low adherent dishes, etc.

[0155] Media used for suspension culture are not particularly limited; however, it is preferable to use serum-free stem cell culture media. A cell group containing cancer stem cells are preferably expanded before performing adherent or suspension culture.

[0156] Herein, “non-adherent” refers to the property of cells to be cultured in a floating state without adherence to culture vessels for suspension culture when the cells are cultured in the vessels.

[0157] “Expansion of a cell group” means, for example, proliferation by spheroid culture or grafting and passaging in nonhuman animals, but is not particularly limited thereto.

[0158] As described herein, for nonhuman animals, immunodeficient animals can be used for grafting since they are unlikely to have rejection reactions. Immunodeficient animals preferably used include nonhuman animals that lack functional T cells, for example, nude mice and nude rats, and nonhuman animals that lack both functional T and B cells, for example, SCID mice and NOD-SCID mice. It is more preferably to use mice that lack T, B, and NK cells and have excellent transplantability, including, for example, NOG mice.

[0159] Regarding the weekly age of nonhuman animals, for example, 4 to 100-week-old athymic nude mice, SCID mice, NOD-SCID mice, or NOG mice are preferably used. NOG mice can be prepared, for example, by the method described in WO 2002/043477, and are available from the Central Institute for Experimental Animals or the Jackson Laboratory (NSG mice).

[0160] Cells to be grafted may be any cells, including cell masses, tissue fragments, individually dispersed cells, cells cultured after isolation, and cells isolated from a different animal into which the cells have been grafted; however, dispersed cells are preferred. The number of grafted cells may be 10⁶ or less; however, it is acceptable to graft more cells.

[0161] With respect to the grafting site, subcutaneous grafting is preferred because the graft technique is simple. The grafting site is not particularly limited, and it is preferable to select an appropriate grafting site depending on the animal used. There is no particular limitation on the grafting operation of NOG-established cancer cell lines, and the cells can be grafted by conventional grafting operations.

[0162] Cancer stem cells or a cancer stem cell population can be prepared, for example, by collecting cancer tissues from patients and culturing the tissues in a serum-free stem cell culture medium under adherent or floating culture conditions. Alternatively, cancer tissues collected from patients can be spheroid-cultured, and then cultured in a serum-free stem cell culture medium under adherent or floating culture conditions to prepare cancer stem cells or a cancer stem cell population Alternatively, cancer tissues collected from patients can be grafted and passaged in nonhuman animals, and then cultured in a serum-free stem cell culture medium under adherent or floating culture conditions to prepare cancer stem cells or a cancer stem cell population. Alternatively, it is possible to use a method in which cancer tissues collected from patients are grafted and passaged in NOG mice to prepare NOG-established cancer cell lines, and they are cultured in a serum-free stem cell culture medium under adherent or suspension culture conditions.

[0163] Cancer stem cells and cancer stem cell populations of the present invention can be used in methods of screening for pharmaceutical agents, anti-cancer agents, or the like.

[0164] In an embodiment of methods of screening for pharmaceutical agents, the present invention provides methods comprising the steps of:

(a) preparing a substantially homogeneous cancer stem cell population comprising an Lgr5-positive adherent cancer stem cell;
(b) contacting a test substance with the cancer stem cell population or a cancer stem cell comprised in the cancer stem cell population; and
(c) detecting a change in a biological property of the cancer stem cell population or cancer stem cell contacted with the test substance.

[0165] In these methods, first, a substantially homogeneous cancer stem cell population containing Lgr5-positive adherent cancer stem cells or Lgr5-negative cancer stem cells is prepared. Then, a test substance is contacted with the prepared cancer stem cell population or cancer stem cells contained in the cancer stem cell population. In these methods, there is no particular limitation on the method for contacting a test substance with a cancer stem cell population or cancer stem cells contained in the cancer stem cell population. For example, a test substance may be contacted with cultured cells of a cancer stem cell population or cancer stem cells contained in the cancer stem cell population. This treatment can be carried out by adding a test substance to a cell culture medium or cell extract. When a test substance is a protein, this treatment can be performed, for example, as follows: a vector comprising a DNA encoding the protein is introduced into a cancer stem cell population or cancer stem cells contained in the cancer stem cell population; or the vector is added to a cell extract of a cancer stem cell population or cancer stem cells contained in the cancer stem cell population. Alternatively, it is possible, for example, to use the two-hybrid method utilizing yeast, animal cells, or the like.

[0166] In these methods, then, a change in a biological property of the cancer stem cell population or cancer stem cells treated with the test substance is detected. Such a change in a biological property includes, for example, a change in the proliferation ability, a change in the viable cell count, a change in a tissue structure characteristic of the process of cancer progression of the cancer stem cell population or cancer stem cells, and a change in the expression of a DNA, RNA, protein, or metabolite in the cancer stem cell population or cancer stem cells. A change in a biological property can be detected, for example, by the methods described below.

[0167] There is no particular limitation on the assessment of the expression of DNAs, RNAs, proteins, peptides, and metabolites; the expression can be assessed by conventional expression assessment methods. RNAs include microRNAs, siRNAs, tRNAs, snRNAs, mRNAs, and non-coding RNAs. For example, mRNAs of a gene are extracted according to a conventional method. Using the mRNAs as a template, the transcriptional level of the gene can be determined using the Northern hybridization or RT-PCR method. DNA array techniques can also be used to determine the expression level of the gene. Alternatively, fractions containing a protein encoded by a gene are collected according to a conventional method. The translational level of the gene can be determined by detecting the protein expression by an electrophoresis method such as SDS-PAGE. The translational level of a gene can also be determined by performing the Western blotting method using an antibody against a protein and detecting the protein expression. These methods can be used to screen for pharmaceutical agents (pharmaceutical compositions).

[0168] The DNAs, RNAs, and proteins that are contained in a cancer stem cell population or cancer stem cells and which are characteristic of the process of cancer progression of the cancer stem cell population or cancer stem cells, preferably include the proteins or polypeptides of any one of SEQ ID NOs: 1 to 6, and polynucleotides encoding the proteins or polypeptides.

[0169] For example, when there is no change in a biological property of a cancer stem cell population or cancer stem cells, or the degree of the change is reduced after treatment with a test substance compared to before the treatment, the test substance is expected to be useful as a pharmaceutical agent (pharmaceutical composition) that has the activity of suppressing cancer recurrence or metastasis (for example, an agent for suppressing cancer recurrence, an agent for post-chemotherapy adjuvant therapy, an agent for postoperative adjuvant therapy, an anti-cancer agent, or an agent for suppressing cancer metastasis). Such test substances can be selected as effective substances that have the therapeutic or preventive effect against cancerous diseases. Such pharmaceutical agents (pharmaceutical compositions) having the activity of suppressing cancer progression are used as an agent for suppressing cancer recurrence, an agent for post-chemotherapy adjuvant therapy, an agent for postoperative adjuvant therapy, an anti-cancer agent, or an agent for suppressing cancer metastasis. Anti-cancer agents of the present invention may be used against, for example, cancers resistant to pharmaceutical agents or chemotherapeutic agents. Specifically, pharmaceutical agents (pharmaceutical compositions) of the present invention also include therapeutic agents against drug-resistant or chemotherapeutic agent-resistant cancers.

[0170] In the present invention, the above pharmaceutical agents (pharmaceutical compositions) are not particularly limited to anti-cancer agents or agents for suppressing metastasis or recurrence, and they can also be used as an agent for inhibiting angiogenesis or cell growth. Furthermore, the pharmaceutical agents (pharmaceutical compositions) of the present invention may be used simultaneously with chemotherapeutic agents or after treatment with chemotherapeutic agents. The pharmaceutical agents are not particularly limited, and they include proteinaceous agents, nucleic acid agents, low-molecular-weight agents, and cellular agents.

[0171] In another embodiment of the screening methods, the present invention provides methods of screening for pharmaceutical agents (pharmaceutical compositions), which comprise the steps of:

(a) preparing a substantially homogeneous cancer stem cell population comprising an Lgr5-negative non-adherent cancer stem cell;
(b) contacting a test substance with the cancer stem cell population or a cancer stem cell comprised in the cancer stem cell population; and
(c) detecting a change in a biological property of the cancer stem cell population or cancer stem cell contacted with the test substance.

[0172] In these methods, first, a substantially homogeneous cancer stem cell population containing Lgr5-negative non-adherent cancer stem cells is prepared. Then, the prepared cancer stem cell population or cancer stem cells contained in the cancer stem cell population are treated with a test substance. Next, a change in a biological property of the cancer stem cell population or cancer stem cells treated with the test substance is detected.

[0173] The DNAs, RNAs, and proteins that are contained in such a cancer stem cell population or cancer stem cells, and which are characteristic of the process of cancer progression of the cancer stem cell population or cancer stem cells, preferably include the proteins or polypeptides of any one of SEQ ID NOs: 1 to 8, and polynucleotides encoding the proteins or polypeptides. In a non-limiting embodiment of the present invention, the proteins or polypeptides of SEQ ID NOs: 1 to 6, and polynucleotides encoding the proteins or polypeptides may be used. In another non-limiting embodiment of the present invention, the protein or polypeptide of SEQ ID NO: 7 or 8, and polynucleotides encoding the protein or polypeptide may be used.

[0174] Pharmaceutical agents (pharmaceutical compositions) that are obtained by the screening methods are not particularly limited, and they can be used as anti-cancer agents. Specifically, when there is no change in a biological property of a cancer stem cell population or cancer stem cells, or the degree of the change is reduced after treatment with a test substance compared to before the treatment, the test substance is expected to be useful as a pharmaceutical agent that has the activity of suppressing cancer recurrence or metastasis (for example, an agent for suppressing cancer recurrence, an agent for post-chemotherapy adjuvant therapy, an agent for postoperative adjuvant therapy, an anti-cancer agent, or an agent for suppressing cancer metastasis). Such test substances may be selected as effective substances that have the therapeutic or preventive effect against cancerous diseases. Such pharmaceutical agents (pharmaceutical compositions) having the activity of suppressing cancer progression are used as an agent for suppressing cancer recurrence, an agent for post-chemotherapy adjuvant therapy, an agent for postoperative adjuvant therapy, an anti-cancer agent, or an agent for suppressing cancer metastasis.

[0175] Furthermore, pharmaceutical agents (pharmaceutical compositions) of the present invention include cancer therapeutic agents against Lgr5-negative cancers, which contain as an active ingredient at least an antibody that binds to a protein of SEQ ID NOs: 1 to 8. The Lgr5-negative cancers include those resistant to drugs or chemotherapeutic agents.

[0176] Still another embodiment of the screening methods of the present invention includes methods that use nonhuman animals administered with a test substance, and a cancer stem cell population of the present invention or cancer stem cells contained in the cancer stem cell population. Specifically, the present invention provides methods of screening for pharmaceutical agents (pharmaceutical compositions), which comprise the steps of:

(a) preparing a substantially homogeneous cancer stem cell population comprising an Lgr5-positive adherent cancer stem cell;
(b) administering a nonhuman animal with a test substance and the cell population or a cancer stem cell comprised in the cancer stem cell population; and
(c) detecting tumor formation in the nonhuman animal.

[0177] In these methods, first, a substantially homogeneous cancer stem cell population containing Lgr5-positive adherent cancer stem cells is prepared. Then, nonhuman animals are administered with a test substance, and the cancer stem cell population prepared or cancer stem cells contained in the cancer stem cell population.

[0178] In these methods, the method for administering a test substance to nonhuman animals is not particularly limited. Oral administration, or parenteral administration such as subcutaneous, intravenous, local, transdermal, or transintestinal (transrectal) administration can be appropriately selected depending on the type of a test substance to be administered.

[0179] Furthermore, in these methods, there is no particular limitation on the method for administering a cancer stem cell population or cancer stem cells to nonhuman animals, and an appropriate method can be selected depending on the cell population to be administered. The preferred method is subcutaneous or intravenous administration.

[0180] In these methods, then, tumor formation is detected in the nonhuman animals.

[0181] The assessment of a test substance can be performed as follows: tissues administered with a test substance and a cancer stem cell population or cancer stem cells are excised from nonhuman animals, and then histological features of the tissues are observed to determine the presence or absence of tumor formation. When tumor formation is not detected, the test substance is expected to be useful as a pharmaceutical agent having the activity of suppressing cancer progression or metastasis (for example, an anti-cancer agent or an agent for suppressing cancer metastasis or recurrence), and the test substance can be selected as an effective substance that has the therapeutic or preventive effect against cancerous diseases. That is, pharmaceutical agents (pharmaceutical compositions) obtained by the screening methods are not particularly limited, and can be used as an anti-cancer agent, or an agent for suppressing cancer metastasis or recurrence.

[0182] “Test substances” used in the methods of the present invention are not particularly limited, and include, for example, single compounds such as natural compounds, organic compounds, inorganic compounds, proteins, antibodies, peptides, and amino acids, as well as compound libraries, expression products of gene libraries, cell extracts, cell culture supernatants, products of fermenting microorganisms, extracts of marine organisms, plant extracts, prokaryotic cell extracts, unicellular eukaryote extracts, and animal cell extracts. These may be purified products or crude purified products such as extracts of plants, animals, and microorganisms. Also, methods for producing test substances are not particularly limited; test substances may be isolated from natural materials, synthesized chemically or biochemically, or prepared by genetic engineering. It is also possible to appropriately use antisense and RNAi molecules that are designed by known methods based on partial sequences of polynucleotides encoding the protein of any one of SEQ ID NOs: 1 to 8. If needed, the above test substances can be appropriately labeled and used. Labels include, for example, radiolabels and fluorescent labels. Mixtures of an above-mentioned test substance and multiple kinds of such labels are included in the test substances of the present invention.

[0183] Furthermore, the present invention provides pharmaceutical agents such as vaccines comprising a partial peptide of the protein of any one of SEQ ID NOs: 1 to 8, and methods of screening for vaccines. Such screening methods preferably include methods for determining the cytotoxic activity targeted to cancer stem cells disclosed in the present application using cytotoxic T cells (CTL) or the like induced with a cancer vaccine of the present invention in vitro. Specifically, adherent and non-adherent cells are separated from peripheral blood mononuclear cells (PBMCs) collected by centrifugation of human peripheral blood in Ficoll-Conray density gradient. The adherent cells are incubated with 100 ng/ml GM-CSF (Novartis) and 10 IU/ml IL-4 (GIBCO-BRL) in AIM-V (GIBCO), and then the cells are used as antigen-presenting cells (APC). Meanwhile, the non-adherent cells are incubated with 30 to 100 IU/ml recombinant IL-4 (Ajinomoto) in AIM-V. On day 7 to 10, a partial peptide of the protein of any one of SEQ ID NOs: 1 to 8 provided by the present invention is added (at a final concentration of 30 μg/ml) to APC. On the following day, recombinant TNF-α and IFN-α (Sumitomo Pharma Co.) are added for APC maturation. Then, CD8-positive cells isolated from autologous non-adherent cells are mixed with irradiated APC in IL-2-free AIM-V. After two days of incubation, IL-2 (Takeda Pharmaceutical Company) is added at a final concentration 100 IU/ml to the culture. The CD8-positive cells are stimulated every seven days using, as APC, autologous PHA blasts (PHA-stimulated T cells) that have been stimulated with the T cell mitogen PHA. A fresh medium containing 100 IU/ml IL-2 is added to the culture at every time point of stimulation. CTL on day 28 is used for the activity assay. High proliferative Lgr5-positive cancer stem cells and low proliferative Lgr5-negative cancer stem cells that are provided by the present invention can be used as target cells of CTL. The cytotoxic activity can be assessed by determining ⁵¹Cr-sodium chromate uptake activity by a measurement method similar to that of ADCC activity.

[0184] Furthermore, pharmaceutical agents selected by the screening methods of the present invention may be further screened as necessary for more effective and practical preventive or therapeutic active substances by conducting additional drug effectiveness tests and safety tests, and further conducting clinical tests in human cancer patients. Based on results of structural analysis of pharmaceutical agents thus selected, they can be industrially manufactured by chemical synthesis, biochemical synthesis (fermentation), or genetic engineering.

[0185] “High proliferative ability” means that the doubling time is 6 days or less, preferably 4 days or less, and more preferably 3 days or less when cells are cultured in a serum-free medium supplemented with EGF and FGF using the method described herein.

[0186] “Low proliferative ability” means that the doubling time is 7 days or more, preferably 14 days or more, and more preferably there is no significant proliferation when cells are cultured in a serum-free medium supplemented with EGF and FGF using the method described herein.

[0187] When preparing such high proliferative Lgr5-positive cancer stem cells and low proliferative Lgr5-negative cancer stem cells, the cells can be separated using the cell marker Lgr5. The separation methods include the following:

methods in which a cell population containing cancer stem cells is isolated by using an anti-Lgr5 antibody;
methods in which a substantially homogeneous cancer stem cell population is first prepared by culturing a population containing cancer stem cells under adherent or suspension culture conditions, and then the population is isolated by using an anti-Lgr5 antibody; and
methods in which a substantially homogeneous cancer stem cell population is first prepared by culturing a population containing cancer stem cells in a medium with or without a growth inhibitor under adherent culture conditions, and then the population is isolated by using an anti-Lgr5 antibody. Any of the above methods may be used in the present invention. Preferably, cells are isolated from cancer tissues after three or more passages in NOG mice, and cultured in a serum-free stem cell culture media under adherent culture conditions to prepare high proliferative Lgr5-positive cancer stem cells. Then, low proliferative Lgr5-negative cancer stem cells can be prepared as follows. The resulting Lgr5-positive cancer stem cells are maintained under various stresses such as a contact with a growth inhibitor, for example, treatment with irinotecan (culture for three days in a serum-free stem cell medium supplemented with 10 μg/ml irinotecan).

[0188] Furthermore, the present invention provides methods of screening for pharmaceutical agents, which comprise contacting a test substance with cancer stem cells that differ in the proliferation ability, which are induced by the methods provided by the present invention. Specifically, the present invention provides methods of screening for pharmaceutical agents, which comprises detecting a change in a biological property of cancer stem cells by contacting a test substance with high or low proliferative cancer stem cells induced by a method for converting low proliferative cancer stem cells to high proliferative cancer stem cells, or converting high proliferative cancer stem cells to low proliferative cancer stem cells.

[0189] Specifically, as described herein, low proliferative cancer stem cells can be prepared by maintaining high proliferative cancer stem cells under various stresses such as a suspension culture or in contact with a growth inhibitor. For example, high proliferative cancer stem cells can be converted to low proliferative cancer stem cells by culturing high proliferative cancer stem cells under suspension culture conditions. Alternatively, high proliferative cancer stem cells can be converted to low proliferative cancer stem cells by culturing high proliferative cancer stem cells in low adherent or ultra-low adherent cell culture vessels such as low adherent plates, ultra-low adherent plates, low adherent dishes, ultra-low adherent dishes, low adherent flasks, or ultra-low adherent cell culture flasks. In other words, low proliferative cancer stem cells can be prepared by culturing high proliferative cancer stem cells in low adherent or ultra-low adherent cell culture vessels such as low adherent plates, ultra-low adherent plates, low adherent dishes, ultra-low adherent dishes, low adherent flasks, or ultra-low adherent cell culture flasks.

[0190] In a non-limiting embodiment, high proliferative cancer stem cells can be converted to low proliferative cancer stem cells by using a growth inhibitor such as 5-FU or irinotecan. Specifically, low proliferative cancer stem cells can be produced by exposing high proliferative cancer stem cells to a growth inhibitor such as 5-FU or irinotecan. Exposure to a growth inhibitor can be achieved under any condition such as in vitro culture or inside the body of grafted nonhuman animals. In this case, those skilled in the art can select an appropriate exposure dose of a cell growth inhibitor for cancer stem cells. Alternatively, high proliferative cancer stem cells can be prepared by re-seeding low proliferative cancer stem cells into a medium without a growth inhibitor such as 5-FU or irinotecan. In another non-limiting embodiment, high proliferative cancer stem cells can be produced by discontinuing administration of a growth inhibitor to nonhuman animals having low proliferative cancer stem cells.

[0191] Furthermore, low proliferative cancer stem cells can be cultured under adherent culture conditions to convert them to high proliferative cancer stem cells. Alternatively, low proliferative cancer stem cells can be converted to high proliferative cancer stem cells by culturing low proliferative cancer stem cells in a non-low-adherent but highly adherent cell culture vessel such as a flat-bottomed plate, plate, adherent culture plate, adherent culture flask, dish, or adherent culture dish. That is, high proliferative cancer stem cells can be produced by culturing low proliferative cancer stem cells in a non-low-adherent but highly adherent cell culture vessel such as a flat-bottomed plate, plate, adherent culture plate, adherent culture flask, dish, or adherent culture dish.

[0192] The present invention also relates to cancer cell detection reagents. Cancer cell detection reagents of the present invention preferably contain as an active ingredient at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8 (protein composed of the amino acid sequence of any one of SEQ ID NOs: 1 to 8). In another embodiment, the reagents of the present invention include reagents for detecting Lgr5-positive cancer cells, which preferably contain at least one antibody that binds to a protein of SEQ ID NOs: 1 to 6 (protein composed of the amino acid sequence of any one of SEQ ID NOs: 1 to 6). In still another embodiment, the reagents of the present invention include reagents for detecting Lgr5-negative cancer cells, which preferably contain at least one antibody that binds to the protein of any one of SEQ ID NOs: 1 to 8 (protein composed of the amino acid sequence of any one of SEQ ID NOs: 1 to 8).

[0193] In a non-limiting embodiment, preferred growth inhibitors include DNA-damaging agents, antimitotic agents, and/or anti-metabolites. Such a DNA-damaging agent may be an alkylating reagent, a topoisomerase inhibitor, and/or a DNA intercalator. Examples of preferred growth inhibitors include, but are not limited to, carboplatin (DNA alkylating reagent), etoposide (topoisomerase II inhibitor), doxorubicin (DNA intercalator), docetaxel (antimitotic agent), and Gemzar (gemcitabine; anti-metabolite).

[0194] Alkylating reagents can be at least one reagent selected from the following. Specifically, it is possible to use at least one alkylating reagent selected from:

chlorambucil, cyclophosphamide, ifosfamide, mechlorethamine, melphalan, uracil mustard, thiotepa, busulfan, carmustine, lomustine, streptozocin, carboplatin, cisplatin, satraplatin, oxaliplatin, altretamine, ET-743, XL119 (becatecarin), dacarbazine, chlormethine, bendamustine, trofosfamide, uramustine, fotemustine, nimustine, prednimustine, ranimustine, semustine, nedaplatin, triplatin tetranitrate, mannosulfan, treosulfan, temozolomide, carboquone, triaziquone, triethylene melamine, procarbazine, etc.

[0195] Topoisomerase inhibitors can be at least one inhibitor selected from the following. Specifically, it is possible to use at least one topoisomerase inhibitor selected from: doxorubicin (Doxil), daunorubicin, epirubicin, idarubicin, anthracenedione (Novantrone), mitoxantrone, mitomycin C, bleomycin, dactinomycin, plicatomycin, irinotecan (Camptosar), camptothecin, rubitecan, belotecan, etoposide, teniposide, topotecan (Hycamptin), etc.

[0196] At least one topoisomerase inhibitor selected from the following can be used as a DNA intercalator:

proflavin, doxorubicin (adriamycin), daunorubicin, dactinomycin, thalidomide, etc.

[0197] Antimitotic agents can be at least one agent selected from the following. Specifically, it is possible to use at least one topoisomerase inhibitor selected from:

paclitaxel (Abraxane)/Taxol, docetaxel (Taxotere), BMS-275183, Xyotax, Tocosal, vinorlebine, vincristine, vinblastine, vindesine, vinzolidine, etoposide (VP-16), teniposide (VM-26), ixavepilone, larotaxel, ortataxel, tesetaxel, ispinesib, etc.

[0198] Anti-metabolites can be at least one inhibitor selected from the following. Specifically, it is possible to use at least one topoisomerase inhibitor selected from:

fluorouracil (5-FU), floxuridine (5-FUdR), methotrexate, Xeloda, Arranon, leucovorin, hydroxyurea, thioguanine (6-TG), mercaptopurine (6-MP), cytarabine, pentostatin, fludarabine phosphate, cladribine (2-CDA), asparaginase, gemcitabine, pemetrexed, bortezomib, aminopterin, raltitrexed, clofarabine, enocitabine, sapacitabine, azacytidine, etc.

[0199] Furthermore, the present invention relates to methods of screening for anti-cancer drugs, which use cancer stem cells isolated or induced by the above methods of the present invention.

[0200] The present invention also relates to methods for assessing compounds, which use cancer stem cells isolated or induced by the above methods of the present invention.

[0201] Furthermore, the present invention provides methods for detecting, identifying, or quantifying the presence of cancer stem cells of the present invention. Specifically, the present invention provides methods for detecting, identifying, or quantifying the presence of cancer stem cells or substantially homogeneous cancer stem cell populations of the present invention, which comprise the steps of:

(a) preparing a sample obtained from a cancer patient; and
(b) contacting a sample with an anti-Lgr5 antibody.

[0202] In these methods, first, samples obtained from cancer patients are prepared. In the present invention, a “sample” is not particularly limited as long as it is preferably an organ or tissue derived from a cancer patient. It is possible to use a frozen or unfrozen organ or tissue. Such samples include, for example, cancer (tumor) tissues isolated from cancer patients. In these methods, a sample is then contacted with an anti-Lgr5 antibody.

[0203] Methods for detecting, identifying, or quantifying the presence of above-described cancer stem cells or substantially homogeneous cancer stem cell populations of the present invention can be used in, for example, cancer diagnosis, selection of cancer patients, prediction or assessment of the effectiveness of an agent (pharmaceutical composition), treatment monitoring, and cancer imaging.

[0204] Specifically, for example, organs or tissues are isolated from cancer patients, and specimens are prepared. The specimens can be used to detect, identify, or quantify the presence of cancer stem cells. Specimens can be appropriately prepared by using known methods, for example, the PFA-AMeX-Paraffin method (WO 09/078386). The samples include, for example, frozen or unfrozen organs or tissues. First, samples from cancer patients are fixed in a PFA solution. “PFA solution” refers to a cell fixation solution which is an aqueous solution of 1 to 6% paraformaldehyde combined with a buffer such as phosphate buffer. It is preferable to use 4% PFA fixation solution (4% paraformaldehyde/0.01 M PBS (pH7.4)). For fixation with a PFA fixation solution, organs or tissues of interest are immersed in a PFA solution containing 1 to 6%, preferably 4% paraformaldehyde, at 0 to 8° C., preferably at about 4° C., for 2 to 40 hours, preferably for 6 to 30 hours. Then, fixed organs or tissues are washed with phosphate buffered saline or such. Washing may be carried out after excising portions from the observed organs or tissues.

[0205] Organs or tissues thus prepared are then embedded in paraffin by the AMeX method. The AMeX method is a paraffin embedding method with a series of the following steps: cold acetone fixation, dehydration with acetone, clearing in methylbenzoate and xylene, and paraffin embedding. Specifically, tissues are immersed in acetone at −25 to 8° C., preferably at −20 to 6° C., for 2 to 24 hours, preferably for 4 to 16 hours. Then, the tissues in acetone are warmed to room temperature. Alternatively, organs or tissues are transferred into acetone at room temperature. Then, dehydration is performed for 0.5 to 5 hours, preferably 1 to 4 hours at room temperature. Subsequently, the organs or tissues are cleared by immersion in methylbenzoate at room temperature for 0.5 to 3 hours, preferably for 0.5 to 2 hours, followed by immersion in xylene at room temperature for 0.5 to 3 hours, preferably 0.5 to 2 hours. Next, the organs or tissues are embedded in paraffin by penetration at 55 to 65° C., preferably at 58 to 62° C. for 1 to 4 hours, preferably for 1 to 3 hours. The paraffin blocks of organs or tissues prepared by the PFA-AMeX method are stored at low temperature before use.

[0206] At the time of use, the paraffin blocks thus prepared are sliced into thin sections using a microtome or the like. Then, the thin sections are deparaffinized and rehydrated. Deparaffinization and rehydration can be performed by known methods. For example, deparaffinization can be performed using xylene and toluene, while rehydration can be carried out using alcohol and acetone.

[0207] The resulting thin sections are stained, for example, by histochemistry, immunohistochemistry, or enzyme histochemistry for detection, identification, or quantitation.

[0208] When the prepared samples are stained by histochemistry (special staining), it is possible to use any staining method commonly available for paraffin-embedded sections (for example, PAS staining, giemsa staining, and toluidine blue staining). For staining by enzyme histochemistry, the sections may be stained by any staining method available for sections (for example, various staining such as with ALP, ACP, TRAP, or esterase). In addition, histopathological tissues can be stained by the following: hematoxylin-eosin staining for general staining; van Gieson staining, azan staining, and Masson Trichrome staining for collagen fiber staining; Weigert staining and Elastica van Gieson staining for elastic fiber staining; Watanabe's silver impregnation staining and PAM staining (periodic acid methenamine silver stain) for reticular fibers/basal membrane staining, etc.

[0209] Staining with immunohistochemistry and enzyme histochemistry can be performed by direct methods using primary antibodies labeled with an enzyme or labeling substance, or indirect methods using non-labeled primary antibodies and labeled secondary antibodies. However, such methods are not limited thereto. Antibodies can be labeled by conventional methods. Labeling substances include, for example, radioisotopes, enzymes, fluorescent substances, and biotin/avidin. The labeling substances may be those commercially available. Radioisotopes include, for example, ³²P, ³³P, ¹³¹I, ¹²⁵I, ³H, ¹⁴C, and ³⁵S. Enzymes include, for example, alkaline phosphatase, horse radish peroxidase, β-galactosidase, and β-glucosidase. Fluorescent substances include, for example, fluorescein isothiocyanate (FITC) and rhodamine. These may be commercially available. Labeling can be carried out by known methods.

[0210] Thin sections are stained, for example, by histochemistry, immunohistochemistry, or enzyme histochemistry for detection, identification, or quantitation.

[0211] Alternatively, detection, identification, or quantitation can be carried out by quantifying DNA or RNA in cells in organ/tissue samples. Assessment of the expression is not particularly limited, and conventional expression assessment methods can be used. RNAs include microRNAs, siRNAs, tRNAs, snRNAs, mRNAs, and non-coding RNAs. For example, Lgr5 mRNA is extracted according to conventional methods. Using the mRNA as a template, the transcriptional level of each gene can be determined by the Northern hybridization or RT-PCR method. DNA array techniques can also be used to determine the expression level of Lgr5.

[0212] Desired tissues, cells, or such can be collected from samples by the microdissection method, in particular, laser microdissection (LMD) method. The LMD method can collect a group of target cells from living tissues, and thus accurately determine which cells express a specific gene among various cells that constitute a tissue, and at what level the cells express the gene. Devices used for microdissection include, for example, the AS-LMD system (Leica Microsystems).

[0213] Furthermore, the present invention provides methods for diagnosing cancer, detecting cancer stem cells, or selecting cancer patients, which comprise using at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8 to detect the presence of at least one of the proteins in a sample isolated from a cancer patient. In order to detect the presence of cancer stem cells, it is possible to use, instead of the anti-Lgr5 antibody described above, at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8.

[0214] In a non-limiting embodiment, the present invention provides methods for diagnosing cancer, detecting cancer stem cells, or selecting cancer patients, which comprise using at least one antibody that binds to a protein of SEQ ID NOs: 1 to 6 to detect the presence of at least one of the proteins in a sample isolated from a cancer patient. In order to detect the presence of Lgr5-positive cancer stem cells, it is possible to use, instead of the anti-Lgr5 antibody described above, at least one antibody that binds to a protein of SEQ ID NOs: 1 to 6. The presence of Lgr5-positive cancer stem cells can be detected by detecting the presence of the protein. However, the present invention does not exclude detection of Lgr5 in addition to the protein.

[0215] In a non-limiting embodiment, the present invention provides methods for diagnosing cancer, detecting cancer stem cells, or selecting cancer patients, which comprise using at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8 to detect the presence of at least one of the proteins in a sample isolated from a cancer patient. In order to detect the presence of Lgr5-negative cancer stem cells, it is possible to use, instead of the anti-Lgr5 antibody described above, at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8. The presence of Lgr5-negative cancer stem cells can be detected by detecting the presence of the protein. However, the present invention does not exclude detection of Lgr5 in addition to the protein.

[0216] Furthermore, the present invention provides methods for assessing the effectiveness of a pharmaceutical composition comprising at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8, which comprise detecting one or more of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins in a sample isolated from a subject administered with the pharmaceutical composition. In these methods, detection may be carried out using at least one antibody that binds to a protein of SEQ ID NOs: 1 to 8, or a portion of a polynucleotide encoding the protein of any one of SEQ ID NOs: 1 to 8 and/or a complementary strand thereof. In another non-limiting embodiment, the present invention provides methods for assessing the effectiveness of a cancer treatment in a test subject, which comprise comparing the expression of at least one of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins in a first sample obtained from a test subject before providing the subject with at least part of the treatment, to the expression of at least one of the proteins of SEQ ID NOs: 1 to 8 and/or polynucleotides encoding the proteins in a second sample obtained from the subject after providing the part of the treatment, wherein a significantly lower level of the protein and/or polynucleotide in the second sample than in the first sample is an indicator showing that the treatment is effective for inhibiting cancer in the test subject.

[0217] In a non-limiting embodiment, the present invention provides methods of monitoring in a test subject the effectiveness of a treatment with an antibody provided by the present invention, which comprise the steps of:

(i) collecting a pre-administration sample from the subject before administration of the antibody;
(ii) determining the expression level of at least one marker protein selected from the proteins of SEQ ID NOs: 1 to 8, or an mRNA or genomic DNA thereof in the pre-administration sample;
(iii) collecting one or more post-administration sample(s) from the subject;
(iv) determining the expression or activity level of at least one marker protein selected from the proteins of SEQ ID NOs: 1 to 8, or an mRNA or genomic DNA thereof in the post-administration sample(s);
(v) comparing the expression or activity level of the marker protein, mRNA, or genomic DNA in the pre-administration sample to that of the marker protein, mRNA, or genomic DNA in the post-administration sample(s); and
(vi) modifying the antibody administration to the test subject according to the comparison.

[0218] For example, an increased dosage of an antibody of the present invention can be used to reduce the expression or activity of a marker towards a level higher than the detected level (the expression or activity level of the marker protein, mRNA, or genomic DNA in the sample before administration), i.e., to increase the effectiveness of the antibody.

[0219] In a different non-limiting embodiment, the present invention provides methods of monitoring in a test subject the effectiveness of a treatment with an antibody provided by the present invention, which comprise the steps of:

(i) detecting an Lgr5-positive cancer stem cell in a pre-administration sample collected from the subject before administration of the antibody;
(ii) determining the expression level of at least one marker protein selected from the proteins of SEQ ID NOs: 1 to 6, or mRNA or genomic DNA thereof in the pre-administration sample;
(iii) collecting one or more post-administration sample(s) from the subject;
(iv) determining the expression or activity level of at least one marker protein selected from the proteins of SEQ ID NOs: 1 to 6, or mRNA or genomic DNA thereof in the post-administration sample(s);
(v) comparing the expression or activity level of the marker protein, mRNA, or genomic DNA in the pre-administration sample to that of the marker protein, mRNA, or genomic DNA in the post-administration sample(s); and
(vi) modifying the antibody administration to the test subject according to the comparison.

[0220] For example, an increased dosage of an antibody of the present invention can be used to reduce the expression or activity of a marker towards a level higher than the detected level (the expression or activity level of the marker protein, mRNA, or genomic DNA in the sample before administration), i.e., to increase the effectiveness of the antibody.

[0221] In another non-limiting embodiment, the present invention provides methods of monitoring in a test subject the effectiveness of a treatment with an antibody provided by the present invention, which comprise the steps of:

(i) detecting an Lgr5-negative cancer stem cell in a pre-administration sample collected from the subject before administration of the antibody;
(ii) determining the expression level of at least one marker protein selected from the proteins of SEQ ID NOs: 1 to 8, or mRNA or genomic DNA thereof in the pre-administration sample;
(iii) collecting one or more post-administration sample(s) from the subject;
(iv) determining the expression or activity level of at least one marker protein selected from the proteins of SEQ ID NOs: 1 to 8, or mRNA or genomic DNA thereof in the post-administration sample(s);
(v) comparing the expression or activity level of the marker protein, mRNA, or genomic DNA in the pre-administration sample to that of the marker protein, mRNA, or genomic DNA in the post-administration sample(s); and
(vi) modifying the antibody administration to the test subject according to the comparison.

[0222] For example, an increased dosage of an antibody of the present invention can be used to reduce the expression or activity of a marker towards a level higher than the detected level (the expression or activity level of the marker protein, mRNA, or genomic DNA in the sample before administration), i.e., to increase the effectiveness of the antibody.

[0223] “Cancer stem cell inhibitor” refers to, for example, an agent having the effect of suppressing the proliferation of cancer stem cells, suppressing the metastasis or recurrence of cancer stem cells, killing cancer stem cells, etc. It may have the effect of suppressing the proliferation of cancer cells, suppressing the metastasis or recurrence of cancer cells, killing cancer cells, etc.

[0224] When used in connection with a biological activity, whose non-limiting examples include the proliferation or metastasis of cancer stem cells, the terms “suppress” and “suppressing”, and synonymous expressions refer to the down-regulation of the biological activity. This can reduce or eliminate a target function such as protein production and phosphorylation of a molecule, etc. In a specific embodiment, the suppression means a decrease in a target activity by about 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 95%. When used in connection with a disorder or disease, the terms refer to the prevention of development of a symptom, relief of a symptom, or successful alleviation of a disease, condition, or disorder.

[0225] “Metastasis” refers to a process where cancer spreads or moves from the primary site to another site in the body, resulting in the development of similar cancer lesions at the new site. “Metastatic cell” or “metastasizing cell” refers to a cell that loses the adhesive contact with adjacent cells, and leaves the primary site of the disease and invades a nearby body structure via blood or lymphatic circulation. “Recurrence” means that the same malignant tumor reappears in a remaining organ after partial resection of an organ for removing a malignant tumor from a cancer patient, or after postoperative chemotherapy following the resection.

[0226] Proteins for use in the present invention can be easily prepared by any method known to those skilled in the art as follows. An expression vector containing a gene comprising a DNA encoding a protein is constructed. The protein is produced and accumulated by culturing transformants transformed with the expression vector. The transformants are harvested to prepare the protein.

[0227] Such an expression vector can be constructed according to methods known in the art, for example, by the following:

(1) excising a DNA fragment that comprises a gene comprising a DNA encoding a protein; and
(2) ligating the DNA fragment downstream of a promoter in an appropriate expression vector.

[0228] Such vectors used include E. coli-derived plasmids (for example, pBR322, pBR325, pUC18, and pUC118), Bacillus subtilis-derived plasmids (for example, pUB110, pTP5, and pC194), yeast-derived plasmids (for example, pSH19 and pSH15), bacteriophages such as λ phage, and animal viruses such as retroviruses, vaccinia viruses, and Baculoviruses.

[0229] Promoters for use in the present invention may be any promoters as long as they are appropriate and compatible with a host to be used for gene expression. For example, when the host is E. coli, preferred promoters include the trp promoter, lac promoter, recA promoter, λPL promoter, and 1pp promoter. When the host is Bacillus subtilis, preferred promoters include the SPO1 promoter, SPO2 promoter, and penP promoter. When the host is yeast, preferred promoters include the PHOS promoter, PGK promoter, GAP promoter, and ADH promoter. When the host is an animal cell, promoters include the SRα promoter, SV40 promoter, LTR promoter, CMV promoter, and HSV-TK promoter.

[0230] In addition to those described above, if desired, enhancers, splicing signals, poly-A addition signals, selection markers, SV40 replication origins, or such known in the art can be added to expression vectors. As necessary, a protein for use in the present invention can be expressed as a fusion protein with another protein (for example, glutathione-S-transferase or Protein A). Such a fusion protein can be cleaved into individual proteins by using an appropriate protease.

[0231] Host cells include, for example, bacteria of the genus Escherichia, bacteria of the genus Bacillus, yeasts, insect cells, insects, and animal cells.

[0232] Specific examples of bacteria of the genus Escherichia include Escherichia coli K12 DH1 (Proc. Natl. Acad. Sci, USA, 60, 160 (1968)), JM103 (Nucleic Acids Research, 9, 309 (1981)), JA221 (Journal of Molecular Biology, 120, 517 (1978)), and HB101 (Journal of Molecular Biology, 41, 459 (1969)).

[0233] Bacteria of the genus Bacillus include, for example, Bacillus subtilis MI114 (Gene, 24, 255 (1983)) and 207-21 (Journal of Biochemistry, 95, 87 (1984)).

[0234] Yeasts include, for example, Saccharomyces cerevisiae AH22, AH22R-, NA87-11A, DKD-5D, and 20B-12; Schizosaccharomyces pombe NCYC1913 and NCYC2036; and Pichia pastoris.

[0235] Animal cells include, for example, monkey COS-7 cells, Vero cells, Chinese hamster CHO cells (hereinafter abbreviated as CHO cells), dhfr gene-deficient CHO cells, mouse L cells, mouse AtT-20 cells, mouse myeloma cells, rat GH3 cells, and human FL cells.

[0236] These host cells can be transformed according to methods known in the art. See, for example, the following references.

• [0237] Proc. Natl. Acad. Sci. USA, Vol. 69, 2110 (1972); Gene, Vol. 17, 107 (1982); Molecular & General Genetics, Vol. 168, 111 (1979); Methods in Enzymology, Vol. 194, 182-187 (1991); Proc. Natl. Acad. Sci. USA), Vol. 75, 1929 (1978); and Virology, Vol. 52, 456 (1973).

[0238] Transformants thus prepared can be cultured according to methods known in the art.

[0239] For example, when hosts were bacteria of the genus Escherichia, in general, they are cultured at about 15 to 43° C. for about 3 to 24 hours. Aeration or stirring is performed as necessary. When hosts are bacteria of the genus Bacillus, in general, they are cultured at about 30 to 40° C. for about 6 to 24 hours. Aeration or stirring is performed as necessary

[0240] When hosts are yeasts, in general, transformants are cultured at about 20° C. to 35° C. for about 24 to 72 hours in a medium adjusted to about pH 5 to 8. Aeration or stirring is performed as necessary.

[0241] When hosts are animal cells, in general, transformants are cultured at about 30° C. to 40° C. for about 15 to 60 hours in a medium adjusted to about pH 6 to 8. Aeration or stirring is performed as necessary.

[0242] To isolate and purify a protein for use in the present invention from the above culture, for example, cells or bacteria are harvested after culture by a known method, and this is suspended in an appropriate buffer. After disrupting the cells or bacteria by sonication, lysozyme, and/or freeze-thawing, a crude protein extract is prepared by centrifugation or filtration. The buffer may contain protein denaturants such as urea and guanidine hydrochloride, and detergents such as Triton X-100™. When the protein is secreted to the culture medium, the supernatant is separated from the cells or bacteria after culture by a known method, and the supernatant is collected. A protein contained in the resulting culture supernatant or extract can be purified by appropriately combining known isolation/purification methods.

[0243] According to known or equivalent methods, a protein prepared as described above can be arbitrarily modified or a polypeptide can be partially removed from the protein by treating the protein produced by recombinants with an appropriate protein modification enzyme such as trypsin and chymotrypsin before or after purification.

[0244] The presence of a protein for use in the present invention can be assessed by various binding assays, enzyme immunoassays using specific antibodies, etc.

[0245] Antibodies for use in the present invention are not particularly limited as long as they bind to proteins for use in the present invention. The antibodies may be obtained as polyclonal or monoclonal antibodies using known methods. Particularly preferred antibodies for use in the present invention include monoclonal antibodies derived from mammals. Monoclonal antibodies derived from mammals include those produced by hybridomas and those produced by hosts transformed with expression vectors carrying antibody genes using gene engineering technologies. It is preferable that antibodies for use in the present invention specifically bind to proteins for use in the present invention.

[0246] Basically, hybridomas producing monoclonal antibodies can be prepared using known techniques by the following procedure. Specifically, immunization is carried out using as a sensitizing antigen a protein for use in the present invention according to conventional immunization methods. The resulting immune cells are fused with known parental cells by conventional cell fusion methods. Monoclonal antibody-producing cells are screened using conventional screening methods. More specifically, monoclonal antibodies can be prepared by the following procedure.

[0247] A gene sequence encoding the protein is inserted into a known expression vector system, and this is transformed into appropriate host cells. Then, the protein is purified from the host cells or culture supernatant by known methods.

[0248] Next, the protein is used as a sensitizing antigen. Alternatively, a partial peptide of the protein is used as a sensitizing antigen. In this case, the partial peptide can be prepared by chemical synthesis based on the amino acid sequence of the protein according to common methods known to those skilled in the art.

[0249] Such a partial polypeptide of the protein has, for example, at least 10 or more amino acids, preferably 50 or more amino acids, more preferably 70 or more amino acids, still more preferably 100 or more amino acids, and yet more preferably 200 or more amino acids of the amino acid sequence constituting the protein, and has, for example, a biological activity substantially equivalent to the function of the protein. The C terminus of the partial peptide is generally a carboxyl group (—COOH) or carboxylate (—COO—); however, the C terminus may also be amide (—CONH₂) or ester (—COOR). In addition, the partial peptides include those in which the amino group of the N-terminal methionine residue is protected with a protecting group, those in which a glutamyl residue resulting from in vivo N-terminal cleavage is pyroglutamine-oxidized, those in which a substituent group in the side chain of an amino acid in the molecule is protected with an appropriate protecting group, and conjugated peptides such as so-called glycopeptides linked with sugar chains.

[0250] Mammals that are immunized with a sensitizing antigen are not particularly limited, though it is preferable to take into consideration compatibility with the parent cell used for cell fusion. Thus, rodents such as mice, rats, or hamsters are generally selected.

[0251] Immunization of animals with a sensitizing antigen is performed according to known methods. For example, standard methods of delivering sensitizing antigen to mammals involve intraperitoneal or subcutaneous injection. More specifically, a sensitizing antigen is diluted to be an appropriate volume with PBS (phosphate-buffered saline), physiological saline, or the like. If desired, this may be mixed with an appropriate amount of a typical adjuvant, for example, Freund's complete adjuvant, made into an emulsion, and then administered to mammals several times every 4 to 21 days. An appropriate carrier may also be used for immunization with sensitizing antigens.

[0252] After the mammals are immunized as described above, an increase in the level of desired antibody in the serum is confirmed, immunocytes are collected from the mammals for cell fusion. Immunocytes that are preferably subjected to cell fusion are splenocytes in particular.

[0253] Regarding the other parent cell to be fused with the above-mentioned immunocytes, mammalian myeloma cells are used. For myeloma cells, it is preferable to use various known cell lines, for example, P3 (P3x63Ag8.653) (J. Immnol. (1979) 123, 1548-1550), P3x63Ag8U.1 (Current Topics in Microbiology and Immunology (1978) 81, 1-7), NS-1 (Kohler, G. and Milstein, C. Eur. J. Immunol. (1976) 6, 511-519), MPC-11 (Margulies, D. H. et al., Cell (1976) 8, 405-415), SP2/0 (Shulman, M. et al., Nature (1978) 276, 269-270), F0 (de St. Groth, S. F. et al., J. Immunol. Methods (1980) 35, 1-21), 5194 (Trowbridge, I. S. J. Exp. Med. (1978) 148, 313-323), and R210 (Galfre, G. et al., Nature (1979) 277, 131-133).

[0254] In general, the above-described immunocytes and myeloma cells can be fused according to known methods, examples of which are described by Kohler and Milstein et al. (Kohler, G. and Milstein, C., Methods Enzymol. (1981) 73, 3-46).

[0255] More specifically, the above-described cell fusion is carried out, for example, in a typical nutrient culture medium in the presence of a cell fusion promoting agent. For example, polyethylene glycol (PEG), Sendai virus (HVJ), or such can be used as the fusion promoting agent. If desired, adjuvants such as dimethylsulfoxide can additionally be used to increase fusion efficiency.

[0256] It is possible to arbitrarily determine the proportion of immunocytes and myeloma cells used. The preferred ratio of myeloma cells to immunocytes is, for example, from 1:1 to 1:10. The culture medium used for the above-described cell fusion may be, for example, RPMI1640 medium, MEM medium, which are suitable for proliferation of the above-described myeloma cell lines, or other kinds of culture medium commonly used for culturing such cells. Furthermore, serum supplements such as fetal calf serum (FCS) may be used in combination.

[0257] The cell fusion is carried out by thoroughly mixing prescribed amounts of the above-described immunocytes and myeloma cells in the aforementioned culture medium, adding to the medium a PEG solution preheated to about 37° C. generally at a concentration of 30% to 60% (w/v), wherein the PEG has an average molecular weight of about 1,000 to 6,000, for example, and mixing them to form the desired fusion cells (hybridomas). An appropriate culture medium is then successively added. Cell fusing agents and such that are undesirable for the proliferation of hybridomas are removed by repeatedly removing the supernatant by centrifugation.

[0258] The hybridomas obtained in this manner are selected by culturing them in a common selection culture medium, for example, the HAT medium (a culture medium containing hypoxanthine, aminopterin, and thymidine). Culture in the HAT medium described above is continued for a sufficient time, usually from a few days to a few weeks, to allow death of all cells but the target hybridomas (the non-fused cells). The usual limiting dilution method is then performed to screen and clone hybridomas producing antibodies used the present invention.

[0259] In addition to methods obtaining the above-described hybridomas by immunizing non-human animals with an antigen, desired human antibodies having an activity of binding to the protein can also be obtained by in vitro sensitizing human lymphocytes with the protein and fusing the sensitized lymphocytes with human-derived myeloma cells having permanent cell division ability (see Japanese Patent Application Kokoku Publication No. (JP-B) H01-59878 (examined, approved Japanese patent application published for opposition)). Furthermore, human antibodies against a protein may be obtained from immortalized antibody-producing cells that are prepared by administering the protein as an antigen to a transgenic animal having a full repertoire of human antibody genes (see, International Patent Applications WO 94/25585, WO 93/12227, WO 92/03918, and WO 94/02602).

[0260] There are known techniques for obtaining human antibodies by panning using a human antibody library. For example, the V regions of human antibodies can be expressed as single-chain antibodies (scFvs) on the surface of phages using a phage display methods, from which phages presenting scFv that binds to an antigen can be selected. The DNA sequences encoding the V regions of human antibodies that bind to the antigen can be determined by analyzing the genes of selected phages. After identifying the DNA sequences of scFvs that bind to the antigen, the V region sequences are fused in frame with the C region sequences of a desired human antibody. Then, the resulting DNA is inserted into an appropriate expression vector to construct an expression vector. The expression vector is introduced into suitable cells for expression, such as those described above. The human antibody can be obtained by expressing the gene encoding the human antibody. These methods are already known (see WO 1992/001047, WO 1992/020791, WO 1993/006213, WO 1993/011236, WO 1993/019172, WO 1995/001438, and WO 1995/015388).

[0261] The hybridomas prepared in this manner that produce monoclonal antibodies can be passaged in a common culture medium and stored for a long time in liquid nitrogen.

[0262] Monoclonal antibodies may be obtained from the hybridomas using common techniques; for example, the hybridomas are cultured according to standard methods and the antibodies may be obtained from the culture supernatants. Alternatively, the hybridomas are administered to a compatible mammal for proliferation and then the antibodies may be obtained from the ascites fluid. The former method is suitable for obtaining highly pure antibodies, while the latter method is suitable for mass production of antibodies.

[0263] Monoclonal antibodies used in the present invention may be recombinant antibodies produced by genetic engineering techniques. They can be produced, for example, by cloning an antibody gene from a hybridoma, incorporating the antibody gene into an appropriate vector, and introducing the resulting vector into a host (see, for example, Vandamme, A. M. et al., Eur. J. Biochem., (1990) 192, p. 767-775, 1990).

[0264] Specifically, mRNAs encoding antibody variable (V) regions are isolated from hybridomas producing the antibodies. mRNAs can be isolated by preparing total RNAs using known methods, for example, guanidine-ultracentrifugation method (Chirgwin, J. M. et al., Biochemistry (1979) 18, 5294-5299), AGPC method (Chomczynski, P. et al., Anal. Biochem. (1987) 162, 156-159), or such. mRNAs of interest are prepared using the mRNA Purification Kit (Pharmacia) or such. The mRNAs can be prepared directly by using the QuickPrep mRNA Purification Kit (Pharmacia).

[0265] The obtained mRNAs are used to synthesize cDNAs of the antibody V regions using reverse transcriptase. cDNAs are synthesized using the AMV Reverse Transcriptase First-strand cDNA Synthesis Kit (Seikagaku Co.) or such. Alternatively, cDNA may be synthesized and amplified following the 5′-RACE method (Frohman, M. A. et al., Proc. Natl. Acad. Sci. USA (1988) 85, 8998-9002; Belyaysky, A. et al., Nucleic Acids Res. (1989) 17, 2919-2932) using the 5′-Ampli FINDER RACE Kit (Clontech) and PCR, and such.

[0266] DNA fragments of interest are purified from the resulting PCR products, and ligated to vector DNAs. From this, a recombinant vector is produced. The recombinant vector is then introduced into E. coli or such, and the desired recombinant vector is prepared from a selected colony. The nucleotide sequences of DNAs of interest are then determined by known methods, for example, the dideoxynucleotide chain termination method.

[0267] A DNA encoding the antibody V region of interest is obtained, and then incorporated into an expression vector carrying a DNA that encodes a desired antibody constant region (C region).

[0268] To produce an antibody used in the present invention, the antibody gene is incorporated into an expression vector so that the gene will be expressed under the control of an expression regulatory region, for example, an enhancer and a promoter. Then, host cells are transformed with the resulting expression vector to express the antibody.

[0269] When expressing antibody genes, a DNA encoding an antibody heavy chain (H chain) or light chain (L chain) can be each separately incorporated into an expression vector to simultaneously transform the host cell, or alternatively DNAs encoding H and L chains can be incorporated into a single expression vector to transform the host cells (see, WO 94/11523).

[0270] Besides the above-described host cells, transgenic animals can also be used to produce recombinant antibodies. For example, an antibody gene is prepared as a fusion gene by inserting the antibody gene into a gene encoding a protein that is specifically produced in milk, such as goat casein. DNA fragments containing the fusion gene to which the antibody gene has been inserted is injected into goat embryos, which are then introduced into female goats. The desired antibody is then obtained from the milk produced by the transgenic goats, which are born from the goats that received the embryos, or from their offspring. Hormones may be suitably given to the transgenic goat to increase the production of milk containing the antibody of interests (Ebert, K. M. et al., Bio/Technology (1994) 12, 699-702).

[0271] In the present invention, in addition to the antibodies described above, artificially modified genetically-recombinant antibodies such as chimeric, humanized, and human antibodies can be used to reduce heterologous antigenicity against humans and such. Such modified antibodies can be produced using known methods. Monoclonal antibodies of the present invention include not only those derived from animals described above but also artificially modified genetically-recombinant antibodies such as chimeric antibodies, humanized antibodies, and bispecific antibodies.

[0272] A chimeric antibody can be obtained by linking a DNA encoding the antibody V region obtained as described above to a DNA encoding the human antibody C region, incorporating this into an expression vector, and then introducing it into a host for production. Useful chimeric antibodies can be obtained using this known method.

[0273] Humanized antibodies are also referred to as “reshaped human antibodies”, which are antibodies obtained by grafting the complementarity determining regions (CDRs) of an antibody from a non-human mammal (e.g., mouse antibody) to the complementarity determining regions of a human antibody. General gene recombination procedures are also known (see, European Patent Application Publication No. EP 125023; International Patent Application Publication No. WO 96/02576).

[0274] Specifically, a DNA sequence designed to link a mouse antibody CDR to the framework region (FR) of a human antibody is synthesized by PCR, using as primers several oligonucleotides prepared to contain overlapping portions in both CDR and FR terminal regions (see methods described in WO 98/13388).

[0275] The human antibody framework region to be linked via CDR is selected such that complementarity determining region forms a favorable antigen-binding site. As necessary, amino acids of the framework region in the antibody variable region may be substituted so that the complementarity determining region of the reshaped human antibody forms a suitable antigen-binding site (Sato, K. et al., 1993, Cancer Res. 53, 851-856).

[0276] Human antibody C-regions are used as the C-regions of chimeric antibodies or humanized antibodies. For example, CH1, CH2, CH3, and CH4 can be used for the H chain, while Cκ and Cλ can be used for the L chain. The human antibody C-region may be modified in order to improve stability of the antibody or its production.

[0277] A chimeric antibody is composed of the variable region of an antibody derived from a non-human mammal and the constant region derived from a human antibody. On the other hand, a humanized antibody is composed of the complementarity determining region of an antibody derived from a non-human mammal, and the framework region and C region derived from a human antibody. Since the antigenicity of humanized antibodies is low in the human body, and humanized antibodies are useful as an active ingredient in therapeutic agents of the present invention.

[0278] Antibodies used in the present invention are not limited to whole antibody molecules, and as long as they bind to proteins used in the present invention, antibody fragments and modification products thereof as well as divalent and monovalent antibodies are also included. Antibody fragments include, for example, Fab, F(ab′)2, Fv, Fab/c having an Fab and the whole Fc, single chain Fv (scFv) in which Fv fragments from H and L chains are ligated via an appropriate linker, and Diabody. Specifically, antibody fragments are prepared by treating antibodies with an enzyme, for example, papain or pepsin. Alternatively, after genes encoding such antibody fragments are constructed and introduced into an expression vector, the antibody fragments are expressed in appropriate host cells using the vector (see, for example, Co, M. S. et al., J. Immunol. (1994) 152, 2968-2976; Better, M. & Horwitz, A. H. Methods in Enzymology (1989) 178, 476-496, Academic Press, Inc.; Plueckthun, A. & Skerra, A. Methods in Enzymology (1989) 178, 476-496, Academic Press, Inc.; Lamoyi, E., Methods in Enzymology (1989) 121, 652-663; Rousseaux, J. et al., Methods in Enzymology (1989) 121, 663-669; Bird, R. E. et al., TIBTECH (1991) 9, 132-137).

[0279] scFv is obtained by ligating antibody H-chain V region with an antibody L-chain V region. In this scFv, the H-chain and L-chain V regions are ligated via a linker, preferably via a peptide linker (Huston, J. S. et al., Proc. Natl. Acad. Sci. U.S.A. (1988) 85, 5879-5883). The H-chain V region and L-chain V region of an scFv may be derived from any of the antibodies described herein. For example, any single-chain peptides consisting of 12 to 19 amino acid residues such as (GGGGS)n may be used as a peptide linker for ligating the V regions.

[0280] A DNA encoding an scFv can be obtained by using, among DNAs encoding the antibody H chain or H chain V region and the antibody L chain or L chain V region mentioned above, all or DNA portion encoding amino acid sequence of interest as a template, amplifying by PCR using a primer pair that defines its two ends; and then carrying out a subsequent amplification using a combination of a DNA encoding the peptide linker portion, and primer pairs that define both ends of the linker DNA to be ligated to the H chain and L chain, respectively.

[0281] Once DNAs encoding scFvs are constructed, expression vectors carrying the DNAs and hosts transformed with the expression vectors can be obtained according to conventional methods. Furthermore, scFvs can be obtained using these hosts according to conventional methods.

[0282] Diabodies are dimers formed by linking two fragments (for example, scFv) in which a variable region is linked to another variable region via a linker or such, and typically have two VLs and two VHs (P. Holliger et al., Proc. Natl. Acad. Sci. USA, 90, 6444-6448 (1993); EP 404097; WO 93/11161; Johnson et al., Method in Enzymology, 203, 88-98, (1991); Holliger et al., Protein Engineering, 9, 299-305 (1996); Perisic et al., Structure, 2, 1217-1226 (1994); John et al., Protein Engineering, 12(7), 597-604 (1999); Holliger et al., Proc. Natl. Acad. Sci. USA., 90, 6444-6448 (1993); Atwell et al., Mol. Immunol. 33, 1301-1312 (1996); and such).

[0283] These antibody fragments can be produced, in a similar manner as described above, by obtaining their genes and expressing them in hosts. Herein, “antibody” comprises such antibody fragments.

[0284] As modified antibodies, antibodies of the present invention linked to various molecules such as polyethylene glycol (PEG) may be used. Moreover, antibodies can also be linked to radioisotopes, chemotherapeutic agents, cytotoxic substances such as bacteria-derived toxins, or such. Herein, “antibody” includes such modified antibodies. Modified antibodies can be obtained by chemically modifying the prepared antibodies. Such antibody modification methods are already established in the art.

[0285] Furthermore, antibodies used in the present invention may be bispecific antibodies. Bispecific antibodies of the present invention may be those having antigen-binding sites that each recognizes different epitopes in the protein used in the present invention or those which recognize the protein used in the present invention and a different protein. Alternatively, bispecific antibodies of the present invention may be those in which one antigen-binding domain recognizes the protein used in the present invention and the other recognizes a chemotherapeutic agent or a cytotoxic substance such as a cell-derived toxin. In this case, proliferation of cancer stem cells can be suppressed by allowing a cytotoxic substance to act directly on cancer stem cells expressing a protein used in the present invention and specifically damaging the cancer stem cells. It is also possible to use bispecific antibodies in which one antigen-binding domain recognizes a molecule that constitutes the T cell receptor complex such as CD3, expressing on cytotoxic T cells, and the other recognizes an epitope in the protein of any one of SEQ ID NOs: 1 to 8 of the present invention. The bispecific antibodies may be prepared by linking pairs of H and L chains from two types of antibodies, or by fusing hybridomas that produce different monoclonal antibodies to yield a fusion cell producing bispecific antibodies. Furthermore, the bispecific antibodies can be prepared using genetic engineering techniques.

[0286] Antibody genes constructed as described above can be expressed and obtained according to known methods. When mammalian cells are used, antibody genes can be expressed using a DNA in which a common useful promoter, an antibody gene to be expressed, and a poly A signal positioned downstream of the antibody gene on the 3′ side are operably linked. Promoter/enhancer includes, for example, human cytomegalovirus immediate early promoter/enhancer.

[0287] Furthermore, other promoter/enhancers that can be used to express the antibody used in the present invention include viral promoter/enhancers of retroviruses, polyoma viruses, adenoviruses, simian virus 40 (SV40), and such; and mammalian cell-derived promoter/enhancers such as human elongation factor 1α (HEF1α).

[0288] When SV40 promoter/enhancer and HEF1α promoter/enhancer is used, gene expression can be easily carried out by the method of Mulligan et al. (Nature (1979) 277, 108) and the method by Mizushima et al. (Nucleic Acids Res. (1990) 18, 5322), respectively.

[0289] Replication origin derived from SV40, polyoma viruses, adenoviruses, bovine papilloma viruses (BPV), and such can be used. Furthermore, to increase the gene copy number in a host cell system, the expression vector may include, as a selection marker, the aminoglycoside transferase (APH) gene, thymidine kinase (TK) gene, E. coli xanthine-guanine phosphoribosyltransferase (Ecogpt) gene, dihydrofolate reductase (dhfr) gene, and such.

[0290] In the case of E. coli, the antibody gene can be expressed by an operably linked common useful promoter, a signal sequence for antibody secretion, and the antibody gene to be expressed. Such promoters include, for example, the lacz promoter and araB promoter. When the lacz promoter or araB promoter is used, the gene can be expressed by the method of Ward et al. (Nature (1098) 341, 544-546; FASEB J. (1992) 6, 2422-2427) or the method of Better et al. (Science (1988) 240, 1041-1043), respectively.

[0291] When an antibody is produced into the periplasm of E. coli, the pel B signal sequence (Lei, S. P. et al., J. Bacteriol. (1987) 169, 4379) may be used as a signal sequence for antibody secretion. After antibodies produced into the periplasm is separated, the antibody structure is appropriately refolded and then used.

[0292] Any expression system that uses, for example, eukaryotic cells or prokaryotic cells may be used to produce antibodies used in the present invention. Eukaryotic cells include, for example, animal cells such as established mammalian cell systems, insect cell systems, cells of filamentous fungi, and yeast cells. Prokaryotic cells include, for example, bacterial cells such as E. coli cells. Antibodies used in the present invention are preferably expressed in mammalian cells, for example, CHO, COS, myeloma, BHK, Vero, and HeLa cells.

[0293] Then, transformed host cells are cultured in vitro or in vivo to produce antibodies of interest. Host cells are cultured according to known methods. For example, DMEM, MEM, RPMI1640, or IMDM may be used as a culture medium, and this may also be used with serum supplements such as fetal calf serum (FCS).

[0294] Antibodies expressed and produced as described above can be isolated from cells or host animals and purified to be homogeneous. Antibodies used in the present invention can be isolated/purified by using affinity columns. For example, Protein A columns include Hyper D, POROS, and Sepharose F. F. (Pharmacia). It is also possible to use other common protein isolation/purification methods. Such methods are not particularly limited. For example, antibodies may be isolated/purified by appropriately selecting/combining chromatography columns other than the above-described affinity columns, filters, ultrafiltration, salting-out, dialysis, and such (Antibodies A Laboratory Manual. Ed Harlow, David Lane, Cold Spring Harbor Laboratory, 1988).

[0295] The antigen-binding activity (Antibodies A Laboratory Manual. Ed Harlow, David Lane, Cold Spring Harbor Laboratory, 1988) and ligand-receptor binding-inhibitory activity (Harada, A. et al., International Immunology (1993) 5, 681-690) of an antibody used in the present invention can be determined by using known methods.

[0296] Enzyme-linked immunosorbent assays (ELISAs), enzyme immunoassays (EIAs), radioimmunoassays (RIAs), and fluorescent antibody methods can be used to determine the antigen-binding activity of the antibody of the present invention. For example, when an enzyme immunoassay is used, samples containing an antibody of the present invention such as a culture supernatant of cells producing the antibody or the purified antibody are added to plates coated with a protein used in the present invention. A secondary antibody labeled with an enzyme such as alkaline phosphatase is added, and the plates are incubated. After washing, an enzyme substrate such as p-nitrophenyl phosphate is added and the absorbance is measured to evaluate the antigen-binding activity.

[0297] An antibody used in the present invention may appropriately be linked to a cytotoxic substance described above such as a proliferation inhibitor, toxic peptide, or radioactive chemical substance. Such modified antibodies (hereinafter referred to as antibody conjugates) can be obtained by chemically modifying the obtained antibodies. Specifically, a linker molecule links a growth inhibitor to an antibody via chemical bonding so that the antibody and growth inhibitor or cytotoxic substance can chemically conjugate with each other (for example, can bind covalently). Preferred binders (linkers) are cleavable linkers. It is more preferable that the linkers are cleaved under mild conditions (specifically, intracellular conditions that do not affect the activity of inhibitors). Examples of suitable cleavable linkers include disulfide linkers, acid-labile linkers, photo-labile linkers, peptidase-labile linkers, and esterase-labile linkers. Disulfide-containing linkers can be cleaved via disulfide exchange, which can occur under physiological conditions. Acid-labile linkers can be cleaved at acid pH. For example, certain intracellular compartments such as endosomes and lysosomes have an acidic pH (pH 4 to 5), and provide conditions suitable for cleaving acid-labile linkers. Photo-labile linkers are useful on the body surface and in many body cavities, which can be exposed to light. Furthermore, infrared light can penetrate tissues. Peptidase-labile linkers can be used to cleave certain peptides inside or outside cells (for example, see Trouet et al., Proc. Natl. Acad. Sci. USA (1982) 79, 626-629; Umemoto et al., Int. J. Cancer (1989) 43, 677-684).

[0298] Such modified antibodies can be prepared not only by chemical modification as described above, but also in a molecular form such as a bispecific antibody designed to recognize a growth inhibitor, toxic peptide, radioactive chemical substance, or the like using genetic recombination techniques. “Antibody” of the present invention also comprises such antibodies.

[0299] Examples of modified antibodies that are provided by the present invention also include those modified with a toxic peptide such as ricin, abrin, ribonuclease, onconase, DNase I, Staphylococcal enterotoxin-A, pokeweed antiviral protein, gelonin, diphtheria toxin, Pseudomonas exotoxin, Pseudomonas endotoxin, L-asparaginase, or PEG L-Asparaginase. In another embodiment, antibodies may be modified by the combined use of one or more growth inhibitors and toxic peptides. As described above, the linkage between the antibody of the present invention that binds to at least one protein described in SEQ ID NOs: 1 to 8 and an above-described growth inhibitor, toxic peptide, or radioactive chemical substance may be a covalent or non-covalent bond. Methods for preparing modified antibodies linked to such chemotherapeutic agents are known.

[0300] Furthermore, proteinaceous pharmaceutical agents and toxins can be linked to an antibody by using a genetic engineering procedure. Specifically, for example, a recombinant vector into which a DNA encoding a toxic peptide described above and a DNA encoding an antibody that binds to any of proteins of at least one of SEQ ID NOs: 1 to 8 of the present invention are fused in frame and incorporated into an expression vector is constructed. Transformed cells obtained by introducing the vector into appropriate host cells are cultured to express the incorporated DNA. The modified antibody linked to the toxic peptide is obtained as a fusion protein. When a fusion protein with an antibody is prepared, in general, a proteinaceous pharmaceutical agent or toxin is placed at the C terminus of the antibody. A peptide linker may be interposed between the antibody and a proteinaceous pharmaceutical agent or toxin.

[0301] Antibodies used in the present invention may have a cytotoxic activity. Herein, the cytotoxic activity includes, for example, complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC). Herein, CDC refers to a cytotoxic activity mediated by the complement system, while ADCC refers to an activity of damaging target cells, which is caused by binding of Fcγ receptor-carrying cells (immunocytes, etc.) via Fcγ receptor to the Fc portion of specific antibody upon binding of the antibody to cell-surface antigens on target cells.

[0302] Whether an antibody used in the present invention has ADCC or CDC can be measured by known methods (see, for example, Current protocols in Immunology, Chapter 7. Immunologic studies in humans, Editor, John E, Coligan et al., John Wiley & Sons, Inc., (1993)).

[0303] Specifically, cytotoxicity can be measured, for example, by the following method.

[0304] Preparation of Effector Cells

[0305] Spleen is removed from a CBA/N mouse or the like, and spleen cells are dispersed in RPMI1640 medium (GIBCO). After washing with the same medium containing 10% fetal bovine serum (FBS, HyClone), effector cells with a cell concentration adjusted to 5×10⁶ cells/ml were prepared.

[0306] Preparation of Complement Solution

[0307] Baby Rabbit Complement (CEDARLANE) is diluted 10-fold with a medium (GIBCO) containing 10% FBS to prepare a complement solution.

[0308] Preparation of Target Cells

[0309] Cells expressing a protein used in the present invention (cancer stem cells, etc.) are radiolabeled by incubating them with 0.2 mCi of ⁵¹Cr-sodium chromate (Amersham Pharmacia Biotech) in DMEM medium containing 10% FBS for one hour at 37° C. After radiolabeled, the cells are washed three times with RPMI1640 medium containing 10% FBS, and the target cells with a cell concentration adjusted to 2×10⁵ cells/ml were prepared.

[0310] ADCC Measurement

[0311] 50 μl the target cells and 50 μl of the antibody used in the present invention are each added to a 96-well U-bottom plate (Becton Dickinson), and reacted for 15 minutes on ice. Thereafter, 100 μl of effector cells are added and incubated in a carbon dioxide incubator for four hours. The final antibody concentration is adjusted to 0 or 10 μg/ml. After incubation, 100 μl of the supernatant is collected and the radioactivity is measured with a gamma counter (COBRAIIAUTO-GMMA, MODEL D5005, Packard Instrument Company). The cytotoxic activity (%) can be calculated according to:

(A−C)/(B−C)×100.

A represents the radioactivity (cpm) of each sample, B represents the radioactivity (cpm) of a sample where 1% NP-40 (nacalai tesque) has been added, and C represents the radioactivity (cpm) of a sample containing the target cells alone.

[0312] CDC Measurement

[0313] 50 μl of the target cells and 50 μl of the antibody used in the present invention are each added to a 96-well flat-bottom plate (Becton Dickinson), and reacted for 15 minutes on ice. Thereafter, 100 μl of the complement solution is added, and incubated in a carbon dioxide incubator for four hours. The antibody final concentration is adjusted to 0 or 3 μg/ml. After incubation, 100 μl of the supernatant is collected to measure the radioactivity with a gamma counter. The cytotoxic activity can be calculated by the similar way as in the ADCC determination.

[0314] Antibodies with modified sugar chains can appropriately be used in the antibodies provided by the present invention. It is known that cytotoxic activity of antibodies can be increased by modifying its sugar chains. Known antibodies with modified sugar chains include, for example: glycosylated antibodies (WO 1999/054342 and such);

antibodies with defucosylated sugar chain (WO 2000/061739, WO 2002/031140, and such); and
antibodies having a sugar chain with bisecting GlcNAc (bisecting N-acetylglucosamine) (WO 2002/079255).

[0315] Antibodies of the present invention preferably include antibodies with modified sugar chains whose sugar chain composition has been altered to increase the ratio of defucosylated antibody or to increase the ratio of antibody attached with bisecting N-acetylglucosamine.

[0316] Antibodies having a neutralizing activity can also be used appropriately in the present invention. In general, “neutralizing activity” refers to the activity of a foreign molecule such as a toxin or virus, or an internal molecule such as a hormone or cytokine to inhibit a ligand's biological activity on cells. Specifically, substance having a neutralizing activity refers to a substance that binds to a ligand or a receptor to which the ligand binds, thereby inhibiting the ligand-receptor binding. The receptor whose ligand binding is inhibited by the neutralizing activity cannot exert their receptor-mediated biological activity. When the antigen-binding molecule is an antibody, in general, such an antibody with a neutralizing activity is called a neutralizing antibody. The neutralizing activity of a test substance can be assessed by comparing biological activities in the presence of a ligand, in the condition of when the test substance is present or not present.

[0317] EREG, which is a target of EP27 antibody described later on in the Examples, is exemplified below. EGF receptor, which is believed to be a main receptor for the EREG represented by SEQ ID NO: 3, dimerizes upon ligand binding and activates its own cytoplasmic tyrosine kinase domain. The activated tyrosine kinase causes a peptide having phosphotyrosine by autophosphorylation, which allows association of various signal transduction accessory molecules. The molecules are mainly phospholipase Cγ (PLCγ), Shc, Grb2, and such. Of these accessory molecules, the former two are further phosphorylated by the tyrosine kinase of the EGF receptor. The main signaling pathway from the EGF receptor is the one in which phosphorylation occurs in order of Shc, Grb2, Sos, Ras, and Raf/MAPK kinase/MAP kinase. It is believed that there is also an alternative pathway from PLCγ to PKC. Since such intracellular signal cascades vary depending on cell type, a target molecule can appropriately be selected for each target cell type of interest and is not limited to the factors described above. It is possible to use an appropriate in vivo signal activation assay kit available on the market (for example, protein kinase C activation measurement system (GE Healthcare Bioscience, etc.)).

[0318] Alternatively, the in vivo signal activation can be detected by using as an indicator the transcriptional induction of a target gene downstream in the in vivo signal cascade. Changes in the transcriptional activity can be detected based on the principle of reporter assay. Specifically, a reporter gene such as GFP (Green Fluorescence Protein) or luciferase is placed downstream of the transcriptional factor or promoter region of the target gene. A change in the transcriptional activity can be determined as reporter activity by measuring the reporter activity.

[0319] In addition, the EGF receptor typically functions to promote cell proliferation, and thus the activation of in vivo signal transduction can be assessed by measuring the proliferative activity of the target cell. In the present invention, the neutralizing activities of neutralizing antibodies of the present invention are assessed by measuring the cell proliferative activity. However, methods are not limited thereto, and methods described above can suitably be used to assess the activity depending on the type of selected target cells.

[0320] Specifically, the neutralizing activity of an anti-EREG antibody can be assessed or determined by measuring the cell proliferative activity, for example, using the method described below. For example, the method where the incorporation of [³H]-labeled thymidine, which is added to a culture medium, into viable cells is measured as an indicator for the DNA replication ability is used.

[0321] Simpler methods include the MTT method and dye exclusion tests in which the ability of cells to exclude dyes such as Trypan Blue outside is measured under a microscope. The MTT method utilizes the ability of viable cells to convert MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), a tetrazolium salt, into a blue formazan product. More specifically, the MTT method is performed as follows: a test antibody is added to a culture medium containing test cells; after a certain period, an MTT solution is added to the culture medium; the mixture is allowed to stand for a certain period so that MMT is incorporated into the cells. As a result, a yellow compound MTT is converted into a blue compound by succinate dehydrogenase in cytoplasmic mitochondria. After the blue product is dissolved for color development, the absorbance is measured as an indicator for the viable cell count.

[0322] In addition to MTT, other commercially available reagents such as MTS, XTT, WST-1, and WST-8 (nacalai tesque, etc.) can preferably be used. There are also known methods for assessing the cell proliferative activity using as an indicator an intracellular ATP content or impedance of cell culture. When assessing the activity, a control antibody that is of the same isotype as the anti-EREG antibody of interest but does not have the neutralizing activity is used in the same manner as for the anti-EREG antibody. The activity can be assessed whether the anti-EREG antibody exhibits the neutralizing activity greater than that of the control antibody.

[0323] Cells whose proliferation is inhibited by the anti-EREG antibody are not particularly limited, as long as they express EREG protein. Examples of preferred EREG-expressing cells include cancer cells. Specifically, cells derived from colorectal cancer, lung adenocarcinoma, pancreatic cancer, stomach cancer, or kidney cancer are preferable EREG-expressing cells in the present invention. A cell proliferation-inhibitory effect against both primary and metastatic lesions of all these cancers can be achieved by the present invention. More preferred cancer cells include those of primary colorectal cancer, metastatic colorectal cancer, lung adenocarcinoma, pancreatic cancer, stomach cancer, and kidney cancer. Thus, anti-EREG antibodies can be used to treat/prevent diseases caused by cell proliferation, for example, colorectal cancer, lung adenocarcinoma, pancreatic cancer, stomach cancer, and kidney cancer. These cancers can be targets of treatment or prevention, regardless of primary or metastatic lesions. More preferably, anti-EREG antibodies are used to treat and/or prevent primary colorectal cancer, metastatic colorectal cancer, and pancreatic cancer. Furthermore, among these cancers, those which grow in an EREG-dependent manner are preferred as a target of treatment and/or prevention in the present invention.

[0324] In the description and Tables herein, when nucleotides and amino acids are represented by abbreviations, these abbreviations are based on the abbreviations by IUPAC-IUB Commission on Biochemical Nomenclature, or the conventional abbreviations in the art. Regarding amino acids, when an optical isomer exists, it represents L form, unless otherwise specified.

[0325] The effective dosage of cancer stem cell inhibitors of the present invention is selected within the range of 0.001 to 1,000 mg/kg weight for each administration. Alternatively, the dosage may be selected within the range of 0.01 to 100,000 mg/body for each patient. However, the dosage of the inhibitors of the present invention is not limited to these doses. Meanwhile, with respect to the timing of administration, an inhibitor of the present invention may be administered before or after manifestation of clinical symptoms of diseases. The inhibitors of the present invention can be formulated according to conventional methods (Remington's Pharmaceutical Science, latest edition, Mark Publishing Company, Easton, US), and may contain both pharmaceutically acceptable carriers and additives. Such carriers and medical additives include, for example, water, pharmaceutically acceptable organic solvents, collagen, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, carboxymethylcellulose sodium, sodium polyacrylate, sodium alginate, water-soluble dextran, carboxymethyl starch sodium, pectin, methyl cellulose, ethyl cellulose, xanthan gum, gum arabic, casein, agar, polyethylene glycol, diglycerin, glycerin, propylene glycol, vaseline, paraffin, stearyl alcohol, stearic acid, human serum albumin (HSA), mannitol, sorbitol, lactose, and surfactants that are acceptable as a medical additive. In practice, additives are selected alone or in appropriate combination from those listed above depending on the dosage form of an inhibitor of the present invention; but are not limited thereto. For example, when used as a preparation for injection, it can be used in a form in which the inhibitor is dissolved in a medium such as physiological saline, buffer, or glucose solution and an adsorption inhibitor such as Tween80, Tween20, gelatin, or human serum albumin is added; alternatively, the inhibitor of the preset invention may be in a lyophilized form for dissolution and reconstitution before use. As excipients for lyophilization, for example, sugar alcohols and saccharides such as mannitol and glucose can be used. The inhibitors of the present invention are generally administered by a parenteral route, for example, via injection (subcutaneous, intravenous, intramuscular, intraperitoneal, etc.), transdermal, transmucosal, intranasal, or pulmonary administration; however, the inhibitor can be administered orally.

[0326] Herein, “combined use” of a cancer stem cell inhibitor and an anti-cancer agent means that these agents may be administered at the same time or in succession; alternatively, one is administered at an interval after administration of the other.

[0327] Herein, cancer stem cell inhibitors can be used as various embodiments such as, for example, prevention of cancer recurrence, suppression of cancer recurrence, prevention of cancer metastasis, suppression of cancer metastasis, and adjuvant therapy for preventing postoperative recurrence for application. When used in the above-described embodiments, any cancer stem cell inhibitor can be used as a cancer stem cell inhibitor of the present invention. However, non-limiting preferred examples include agents for inhibiting cancer stem cell proliferation or agents for disrupting cancer stem cell. As long as agents for inhibiting cancer stem cell proliferation that are provided by the present invention can suppress the proliferation of target cancer stem cells, mechanism of suppressing cancer stem cell proliferation is not relevant. As a non-limiting example, such agents for inhibiting cancer stem cell proliferation include those comprising as an active ingredient an antibody having neutralizing activity against cancer stem cell proliferation or growth or an antibody having cytotoxicity against cancer stem cells. Similarly, as long as agents for disrupting cancer stem cells that are provided by the present invention can destroy target cancer stem cells, mechanism of disrupting cancer stem cells is not relevant. As a non-limiting example, such agents for disrupting cancer stem cells include agents for inhibiting cancer stem cell proliferation comprising as an active ingredient an antibody having cytotoxicity or apoptotic activity against cancer stem cells. Those skilled in the art can determine whether a test agent for disrupting cancer stem cells has apoptotic activity by using known methods including terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assay, caspase activity (in particular, caspase-3) assay, fas ligand assay, and Annexin V assay as apoptotic activity assay methods. Non-limiting preferred examples include cancer stem cell differentiation enhancers. Non-limiting examples of differentiation enhancers include BMP4, i.e., the polypeptide of SEQ ID NO: 9, or polypeptide equivalents having one or several amino acid addition(s), deletion(s), and/or substitution(s) among amino acids of the polypeptide. Such polypeptide equivalents preferably have a CSC differentiation-inducing activity equivalent to that of the polypeptide of SEQ ID NO: 9. The equivalency of the differentiation-inducing activity can be defined, for example, by whether the CK20-inducing activity for CSCs is 10%, preferably 20%, more preferably 30%, even more preferably 40%, and still more preferably 50% of that of the polypeptide of SEQ ID NO: 9. In another non-limiting embodiment, the equivalency of the differentiation-inducing activity can be defined, for example, by whether the CK20-inducing activity for CSCs is 60%, preferably 70%, more preferably 80%, even preferably 90%, and still preferably 95% of that of the polypeptide of SEQ ID NO: 9.

[0328] Anti-cancer agents that are used in combination with a cancer stem cell inhibitor of the present invention include alkylating agents, metabolic antagonists, natural products, platinum complexes, and other pharmaceutical agents. Alkylating agents include nitrogen mustards, ethylenimines, methylmelamines, alkyl sulfonates, nitrosoureas, and triazens. Nitrogen mustards include, for example, mechlorethamine, cyclophosphamide, ifosfamide, melphalan, and chlorambucil. Ethylenimines and methylmelamines include, for example, hexamethylmelamine and thiotepa. Alkyl Sulfonates include busulfan. Nitrosoureas include, for example, carmustine (BCNU), lomustine (CCNU), semustine (methyl-CCNU), and streptozocin. Triazens include dacarbazine (DTIC). Metabolic antagonists include folic acid analogs, pyrimidine analogs, and purine analogs. Folic acid analogs include methotrexate. Pyrimidine analogs include, for example, fluorouracil (5-FU), doxifluridine (5′-DFUR; trade name: FURTULON), capecitabine (trade name: Xeloda), floxuridine (FudR), and cytarabine. Purine analogs include, for example, mercaptopurine (6-MP), thioguanine (TG), and pentostatin. Natural products include vinca alkaloids, epipodophyllotoxins, and antibiotics. Vinca alkaloids include, for example, vinblastine (VLB) and vincristine (VCR). Epipodophyllotoxins include, for example, etoposide and teniposide. Antibiotics include, for example, dactinomycin (actinomycin D), daunorubicin, doxorubicin, bleomycin, plicamycin, and mitomycin. Platinum complex refers to platinum coordination complex, and includes, for example, cisplatin (CDDP) and carboplatin. Other pharmaceutical agents include: topoisomerase inhibitors such as irinotecan and camptothecin; taxols, for example, paclitaxel, docetaxel; anthracenediones, for example, mitoxantrone; urea-substituted derivatives, for example, hydroxyurea; methyl hydrazines, for example, procarbazine hydrochloride (trade name: Natulan), vitamin A metabolites, for example, tretinoin (trade name: VESANOID), as well as include rituximab, alemtuzumab, trastuzumab, bevacizumab, cetuximab, panitumumab, trastuzumab, and gemutuzumab.

[0329] All prior-art documents cited herein are incorporated herein by reference.

[0330] Herein below, the present invention will be specifically described with reference to examples, however, it is not to be construed as being limited thereto.

Establishment of Human Colorectal Cancer Cell Lines with Immunodeficient NOG Mice

[0331] Colorectal cancer specimens were obtained from patients with their consent under approval of the ethical committees of PharmaLogicals Research (Singapore) and Parkway Laboratory Services (Singapore). Tumor blocks were cut into small pieces with a razor blade, and grafted in the lateral region of NOG mice. Human colorectal cancer xenografts were maintained by passaging them in NOG mice provided by the Central Institute for Experimental Animals (Japan). Mice used in this experiment were treated in accordance with the animal experiment guidelines of PharmaLogicals Research. For histopathological examination, small blocks of xenograft tumors and surgical human tissue samples were fixed with 4% paraformaldehyde at 4° C. for 16 to 24 hours, and embedded in paraffin by the AMeX method (Sato Y, et al., (1986) Am J Pathol, 125: 431-435; Sato Y, et al., (1992) Am J Pathol, 140: 775-779; Suzuki M, et al. (2002) J Toxicol Sci, 27: 165-172). The thin sections were stained with eosin and hematoxylin and assessed by microscopic observation.

[0332] Tissues of xenograft were cut with a razor blade in order to prepare single cancer cell suspensions. After the suspensions were incubated at 37° C. for 3 hours in DPBS containing collagenase/dispase (Roche) and DNaseI (Roche), the suspensions were filtered through 40-μm Cell Strainers (BD Biosciences). The cells were suspended in the lysis buffer (BD Biosciences) to remove erythrocytes. The prepared xenograft-derived cells (such cells are referred to as primary cells) were cultured under 5% CO₂ atmosphere at 37° C. in DMEM/F12 (Invitrogen) containing N-2 Supplement (Invitrogen), 20 ng/ml human EGF (Invitrogen), 10 ng/ml human basic fibroblast growth factor (Sigma), 4 μg/ml heparin (Sigma), 4 mg/ml BSA (Invitrogen), 20 μg/ml human insulin zinc solution (Invitrogen), and 2.9 mg/ml glucose (Sigma) (Todaro M, et al. (2007) Cell Stem Cell 1: 389-402). Adherent and floating CSCs were cultured using conventional polystyrene-treated cell culture flasks (BD Biosciences) and Ultra-low attachment cell culture flasks (Corning), respectively.

[0333] Cell suspensions were prepared by serial dilution. 100 μl of cancer cell suspensions in Hanks' balanced salt solution (Invitrogen) were subcutaneously inoculated into lateral region of mice using 50% matrigel (BD Bioscience). The tumor development was monitored over seven weeks. In order to inoculate single cell, cells were labeled using an FITC-labeled mouse anti-human CD326 (EpCAM) antibody (Miltenyi Biotec), and plated in a terasaki plate (Termo Fisher Scientific). Cell singularity was confirmed under a microscope. Single cells were inoculated into lateral region of mice using 50 μl of 50% matrigel. The tumor development was monitored over 10 weeks.

[0334] Full-length human Lgr4, Lgr5, and Lgr6 cDNAs were cloned by PCR based on the sequences of NM_018490 (Lgr4), NM_001017403 (Lgr6), and NM_003667 (Lgr5). The cloned genes were expressed with or without adding HA tag to their N termini. Cells of Chinese hamster ovary cell line CHO DG44 (Invitrogen) were transfected with expression plasmids using Gene Pulser (BioRad). Stable cell lines HA-Lgr4/DG, HA-Lgr5/DG, and HA-Lgr6/DG were selected using G418.

[0335] Soluble Lgr5 protein (amino acids 1 to 555) was expressed as a fusion protein with the Fc domain of mouse IgG2a in CHO DG44. Transfectants were screened by sandwich ELISA using a goat anti-mouse IgG2a (Bethyl laboratories) and HRP-rat anti-mouse IgG2a mAb (Serotec). A clone that produces sLgr5-Fc at the highest level was named 2D3. A culture supernatant of 2D3 was collected, and Lgr5-Fc protein was affinity-purified using a Protein A-Sepharose column (Pharmacia). Lgr5-Fc was used as an antigen in protein immunization and ELISA screening.

Generation of Anti-Lgr5 Monoclonal Antibody by Immunization with Lgr5-Fc Protein

[0336] Balb/c mice (Charles River Japan) were immunized subcutaneously with 50 μg of Lgr5-Fc emulsified in Freund's complete adjuvant. After two weeks, the mice were injected with the same amount of Lgr5-Fc in Freund's incomplete adjuvant once a week over two weeks. Three days before cell fusion, 25 μg of Lgr5Fc was intravenously injected to the mice. Spleen lymphocytes derived from the immunized mice were fused with cells of mouse myeloma line P3-X63Ag8U1 (ATCC) using a conventional method (Kremer L and Marquez G (2004) Methods Mol Biol., 239: 243-260). Hybridoma culture supernatants were screened for antibodies reactive to sLgr5-Fc by ELISA to establish Lgr5-specific mouse mAb 2T15E-2 and 2U2E-2.

[0337] Large intestine CSCs were incubated with labeled antibodies and analyzed using the EPICS ALTRA (Beckman Coulter) and FACSCalibur (Becton Dickinson)). Antibodies used were: PE-labeled mouse anti-human CD133 antibody (Miltenyi Biotec), PE-labeled mouse anti-human CD44 antibody (BD Pharmingen), FITC-labeled mouse anti-human CD326 (EpCAM) antibody (Miltenyi Biotec), PE-labeled mouse anti-human CD166 antibody (R&D Systems), PE-labeled mouse anti-human CD24 antibody (BD Pharmingen), PE-labeled mouse anti-human CD26 antibody (BD Pharmingen), and PE-labeled mouse anti-human CD29 antibody (BD Pharmingen).

[0338] Large intestine CSCs were incubated with mouse anti-human Lgr5 antibody (2T15E-2) and then with PR-labeled rat anti-mouse IgG antibody (Invitrogen) to stain Lgr5. The aldehyde dehydrogenase activity was measured using the AldeFluor Kit (Stemcell Technologies). Mouse cells were discriminated from human large intestine CSCs by staining with anti-mouse MHC class I antibody (Abcam), and PE- or APC-labeled goat anti-human IgG2a antibody (BioLegend). Dead cells were also removed using the 7-AAD Viability Dye (Beckman Coulter).

[0339] Protein was extracted using RIPA buffer (Sigma) supplemented with the Complete Mini Protease Inhibitor Cocktail (Roche). Proteins were fractionated by the NuPAGE Gel (Invitrogen) and transferred onto PVDF membrane. After blocking with PBS containing 1% skimmed milk, the membrane was probed with rabbit anti-human β-catenin antibody (Sigma), rabbit anti-human phospho-c-JUN antibody (Sigma), rabbit anti-human TCF1 antibody (Cell Signaling), rabbit anti-human TCF3 antibody (Cell Signaling), rabbit anti-human TCF4 antibody (Cell Signaling), rabbit anti-human Lgr5 antibody (Abcam), mouse anti-human E-cadherin antibody (Abcam), rabbit anti-human Snail antibody (Abcam), and mouse anti-human GAPDH antibody (Santa Cruz). Reactive bands were detected using BCIP/NBT substrate (KPL).

[0340] Total RNAs were isolated using the RNeasy Mini Kit including DNAase treatment (Qiagen). cDNAs were synthesized using the First-Strand cDNA Synthesis Kit (SABiosciences). Quantitative real-time PCR (QRT-PCR) analysis was performed with the SYBR Green/Rox qPCR (SABiosciences) using the Mx3005P Real-Time PCR System (Stratagene). The fold induction value was calculated according to the 2-AACt method. GAPDH and ACTB were used as a reference. All experiments were performed in triplicate.

[0341] The following primers were used to amplify reactive transcripts.

Lgr5:
(SEQ ID NO: 10)
forward primer:	5′-AGTTTATCCTTCTGGTGGTAGTCC-3′;
(SEQ ID NO: 11)
reverse primer:	5′-CAAGATGTAGAGAAGGGGATTGA-3′;
GAPDH:
(SEQ ID NO: 12)
forward primer:	5′-CTCTGCTCCTCCTGTTCGAC-3′;
(SEQ ID NO: 13)
reverse primer:	5′-ACGACCAAATCCGTTGACTC-3′;
ACTB:
(SEQ ID NO: 14)
forward primer:	5′-AAGTCCCTTGCCATCCTAAAA-3′;
(SEQ ID NO: 15)
reverse primer:	5′-ATGCTATCACCTCCCCTGTG-3′

[0342] Floating and adherent CSCs were plated at about 100 and 1×10⁴ cells/well in 96-well plates, respectively. On days 0 and 3, viable cell counts were determined by the Cell Counting Kit-8 Assay (Doujindo) according to the manufacturer's protocol. Average absorbance on day 0 was taken as 100%. For chemosensitivity assay, floating and adherent CSCs were plated at about 100 and 1×10⁴ cells/well in 96-well plates, respectively. After 24 hours of incubation, 10 μg/ml 5-FU (Hospira), 10 μg/ml irinotecan (Hospira), 50 mM TCF inhibitor FH535 (Merck), or 50 mM β-catenin inhibitor Cardamonin (Merck) were added to the plates. After three days of culture in the presence of the agents, the Cell Counting Kit-8 was added to the cells. The average absorbance of cells exposed to DMSO or medium alone was taken as 100%. All experiments were performed in triplicate.

[0343] For immunofluorescent cytochemistry, cells were fixed with 4% paraformaldehyde and methanol, and incubated with a mouse anti-human E-cadherin antibody (Abcam), rabbit anti-human Snail antibody (Abcam), or rabbit anti-human β-catenin antibody (Sigma)). Then, the cells were visualized using the AlexaFluor 488-labeled goat anti-mouse IgG antibody or goat anti-rabbit IgG antibody. For immunofluorescent cytochemistry, thin sections derived from paraffin blocks of xenograft tumors described above were incubated with a mouse anti-human Lgr5 antibody (2U2E-2) or rabbit anti-human Snail antibody (Abcam). After incubation with a primary antibody, Lgr5 protein was detected with a goat anti-mouse antibody conjugated with the polymer-HRP (DAKO) and visualized with the AlexaFluor 488-labeled tyramide (Invitrogen), while Snail protein was detected with biotinylated goat anti-rabbit antibody (VECTOR) and visualized with the AlexaFluor 568-labeled streptavidin (Invitrogen). These cells and samples were also stained with DAPI (Invitrogen).

[0344] As described in a previous report (Fujii E. et al., (2008) Establishment and characterization of in vivo human tumor models in the NOD/SCID/gamma(c)(null) mouse. Pathol Int 58: 559-567), the present inventors established 11 types of human colorectal cancer xenografts using NOD/Shi-scid, IL-2Rγnull (NOG) mice (Table 1; the number of human colorectal cancer cell lines established with immunodeficient NOG mice).

	TABLE 1
	Adenocarcinoma
	G1	G2	G3	Total
	Number of cases	4	46	3	53
	Established	0	10	1	11
	Impracticable*	0	6	0	6
	EBV lymphoma	2	16	1	19
	Aggravated animal condition†	1	12	1	14
	No viable cancer	1	2	0	3

[0345] In the above Table 1, asterisk indicates cases established but unsuitable for experiment, and dagger indicates cases with infection and such.

[0346] As shown in Table 1, 17 types of colorectal cancer xenografts were established from samples of 53 human colorectal cancer patients. Except for the 17 types of xenografts, associated EBV-infected lymphoma cells occurred in 19 cases (which aggravated the condition of NOG mice); other infections were found in 14 cases; and no tumor growth was observed in three cases. Of the 17 types, 11 xenografts survived even after freeze-thawing, and had the capacity to reconstitute tumor, and showed similar histopathological features as those of the original tumors. Of the 11 types, 10 xenografts were derived from grade-2 moderately-differentiated adenocarcinomas, and the remaining one was derived from a grade-3 poorly-differentiated adenocarcinoma.

[0347] Of the 11 types, 10 xenografts were derived from moderately-differentiated colorectal cancer (MDCC), and the remaining one was derived from poorly-differentiated colorectal cancer (PDCC) (Table 2; histopathological classification of the original human colorectal cancers that were used to establish the 11 xenografts).

	TABLE 2
	Histopathological classification of original human tumor
Line				AJCC	Dukes'
No.	Type	Grade	TNM	stage	stage
PLR30	Adenocarcinoma	G2	pT3NOMX	II	B
PLR59	Adenocarcinoma	G2	pT3N2MX	III	C2
PLR123	Adenocarcinoma	G2	pT4N1MX	III	C1
PLR168	Adenocarcinoma	G2	pT3NOMX	II	B
PLR215	Adenocarcinoma	G2	pT3NOMX	II	B
PLR241	Adenocarcinoma	G2	pT4N3M1	IV	D
PLR254	Adenocarcinoma	G2	pT4N2MX	III	C2
PLR261	Adenocarcinoma	G2	pT3NOMX	II	B
PLR325	Adenocarcinoma	G3	pT4N1M1	III	C1
PLR379	Adenocarcinoma	G2	pT4N2MX	IIIC	C1
PLR423	Adenocarcinoma	G2	pT3NOM1	IV	D

[0348] Both MDCC and PDCC xenografts reconstituted histopathological morphologies almost equivalent to those of the original tumors. MDCC xenografts formed specific epithelial ducts which contained goblet cells, and small budding clusters (may undergo epithelial-mesenchymal transition (EMT)). In contrast, PDCC xenografts did not form such specific epithelial duct structures (FIGS. 1 and 16).

[0349] In order to isolate large intestine CSCs, the present inventors used two types of MDCC xenografts, i.e., PLR59 and PLR123. These xenografts were chosen by the present inventors because they grew rapidly even after 10 passages in NOG mice while maintaining the capacity to reconstitute tumors with epithelial ducts and small budding clusters (FIG. 1). Thus, the present inventors predicted that stable CSCs can be obtained from the xenografts.

[0350] Flow cytometry analysis of primary cells passaged in NOG mice derived from PLR59 and PLR123 showed that signal level of CD44, ALDH, CD26, and Lgr5 were lower than that of CD133, EpCAM, CD166, CD24, and CD29. This suggests the existence of a small population of CSCs (FIG. 2). Indeed, when primary cells derived from PLR59 and PLR123 were grafted subcutaneously to NOG mice at 100 cells/injection site, tumors were formed at half of the injection sites (five of 12 injection sites; Table 3), and the histopathological morphology of tumors is highly similar to that of original tumors in that they had hierarchical organization (FIG. 3). However, primary cells derived from PLR59 and PLR23 were injected subcutaneously at 10 cells/site, any tumor was not formed in NOG mice (Table 3). Table 3 shows the cancer formation ability 49 days after inoculation.

	TABLE 3
		Number of cells/
	Cell	inoculation site
	line*	Specimen†	1,000	100	10
	PLR59	Primary	12+/12‡	5/12	0/12
			(100)	(42)	(0)
		Non-adherent	6/6	6/6	1/6
		(Lgr5−)	(100)	(100)	(17)
		Adherent (Lgr5+)	6/6	6/6	6/6
			(100)	(100)	(100)
	PLR123	Primary	12/12	5/12	0/12
			(100)	(42)	(0)
		Non-adherent	6/6	5/6	2/6
		(Lgr5−)	(100)	(83)	(33)
		Adherent (Lgr5+)	6/6	6/6	6/6
			(100)	(100)	(100)

[0351] In Table 3 shown above, asterisk indicates tumor xenografts established with NOG mice, and dagger indicates cell preparations. Primary indicates cells (primary cells) prepared by harvesting xenograft tumor tissues grown in NOG mice and removing erythrocytes and mouse cells. Floating indicates cells prepared by in vitro culturing primary cells under a non-adherent culture condition. Adherent indicates cells prepared by in vitro culturing primary cells under adherent culture conditions. Plus symbol (single) indicates the number of formed tumors, while plus symbol (double) indicates the total number of inoculation sites. Parenthesis indicates percent tumor (cancer) formation. Lgr5⁺ represents Lgr5 positive, and Lgr5⁻ represents Lgr5 negative.

[0352] Primary cells derived from PLR59 and PLR123 were cultured in serum-free media supplemented with EGF and FGF. This yielded adherent and non-adherent cells. The present inventors harvested the adherent and non-adherent cells and cultured them separately. The adherent cells were grown with a doubling time of about 2.5 days and exhibited mesenchymal cell-like morphology. The non-adherent cells, on the other hand, formed spheroid-like cell clusters but did not proliferate significantly (FIGS. 4, 5, 18, and 19). After one-week or longer culture, the cells were assessed for large intestine CSC markers. This demonstrated that both adherent and non-adherent cells were highly homogeneous. The adherent cells were of Lgr5⁺, ALDH⁺, CD133⁺, CD44⁺, EpCAM⁺, CD166⁺, CD24⁺, CD26⁺, and CD29⁺. Meanwhile, the non-adherent cells were of Lgr5⁻ and ALDH⁻, and thus were different from the adherent cells (FIGS. 6 and 20).

[0353] Lgr5 mRNA was detected at a significant level in the adherent cells, while it was undetectable in non-adherent cells (FIG. 27).

[0354] In order to assess the expression of Lgr5 protein, the present inventors prepared two types of Lgr5-specific monoclonal antibodies (2L36, 2T15E-2, and 2U2E-2) respectively for immunohistochemistry and flow cytometry analysis. The antibodies produced by the present inventors were highly specific to Lgr5 without any cross-reactivity to Lgr4 or Lgr6, both of which are highly homologous to Lgr5 (FIGS. 28 and 29). Using the antibodies, the present inventors demonstrated the expression of Lgr5 in the adherent CSCs.

[0355] Lgr5-positive cells were detected in tumor tissues that were the origins of PLR59 and PLR123 as well as in xenograft cancer tissues therefrom through all passages (FIG. 39). The frequency of Lgr5-positive cells was low in the original tumor tissues (0.01% and 0.04% for PLR59 and PLR123, respectively). Regarding the xenograft cancer tissues, the frequency of Lgr5-positive cells was increased as passage number increased; however, there was no further change after tenth generation (FIG. 39). On the other hand, the tumor-reconstituting capacity of primary cells from PLR123 xenograft model was also potentiated as passage number increased. The ratio of CSCs in the primary cells, which was estimated based on the capacity to reconstitute tumor, was about 0.1% after 5 passages, and was increased to about 0.4% after 14 passages.

[0356] If a group of colorectal cancer stem cells is characterized by Wnt signaling, Lgr5-positive adherent cells alone can form tumors in vivo. To confirm whether this prediction is true, the present inventors assessed the tumor-forming capacities of Lgr5-positive adherent cells and Lgr5-negative non-adherent cells.

[0357] The result showed that Lgr5-positive adherent cells were more potent than Lgr5-negative non-adherent cells in the capacity to form tumors. However, both Lgr5-positive and Lgr5-negative cells had the capacity to form tumors in NOG mice. Subcutaneous injection of ten Lgr5-positive cells caused tumor formation at every injection site (six of six sites), while Lgr5-negative cells formed tumors at two of six injection sites (PLR123-derived cells) or at a single site (PLR59-derived cells) (Table 3). Lgr5-positive cells, even when injected at only one cell per inoculation site, reconstituted tumors at two of 12 injection sites (PLR123-derived cells) or at a single site (PLR59-derived cells) (FIG. 7). The histopathological morphologies of tumors derived from the Lgr5-positive and Lgr5-negative cells were almost the same as those of the original tumors (FIGS. 17 and 40). Furthermore, there was no change in the expression of cell surface markers and tumor-initiating activity of the Lgr5-positive CSCs even after one month of passage culture (FIGS. 30 and 31).

[0358] Under adherent culture conditions, the Lgr5-positive cells underwent symmetric cell division (FIG. 41). Meanwhile, in the presence of matrigel and serum, Lgr5 protein was distributed to one of two daughter cells under the same culture conditions (FIGS. 42C and 42D), demonstrating that the Lgr5-positive cells undergo asymmetrical cell division. One of CSC's properties is symmetrical cell division for self-renewal, and another characteristic property is asymmetrical cell division. The Lgr5-positive adherent cells divided symmetrically under adherent culture conditions (FIG. 41), whereas, in the presence of matrigel and FBS, as seen from the fact that Lgr5 protein was distributed to one of daughter cells (FIG. 42), the Lgr5-positive cells underwent asymmetrical cell division, which resulted in two distinct progenies.

[0359] The results described above demonstrate that Lgr5-positive and Lgr5-negative cells derived from PLR59 and PLR123 are highly pure large intestine CSCs, and that the Lgr5-positive and Lgr5-negative cells correspond to two independent CSC states in colorectal cancer.

[0360] In the Lgr5-positive cells, the levels of β-catenin, TCF1, TCF3, and TCF4 proteins were upregulated in accordance with the expression of Lgr5. This was not observed in the Lgr5-negative cells (FIGS. 7 and 21).

[0361] On the other hand, the N-terminal phosphorylation of c-Jun was undetectable in the Lgr5-positive CSCs as compared to the Lgr5-negative CSCs (FIGS. 7 and 21).

[0362] To answer the question of whether Wnt signaling promotes the proliferation of large intestine CSCs, the present inventors assessed the effects of β-catenin/TCF inhibitor FH535 and Wnt/β-catenin inhibitor cardamonin (which induces β-catenin degradation) on the proliferation of large intestine CSCs.

[0363] The result showed that 50 μM FH535 significantly reduced the proliferation of Lgr5-positive large intestine CSCs but had no effect on the proliferation of Lgr5-negative large intestine CSCs (FIGS. 8 and 22). Meanwhile, 50 μM cardamonin reduced the viable cell count to 70% for the Lgr5-positive large intestine CSCs and to about 50% for the Lgr5-negative large intestine CSCs (FIGS. 8 and 22).

[0364] This result suggests that TCF mediates the proliferation of Lgr5-positive cells and that β-catenin is involved in the survival of large intestine CSCs. Interestingly, the Lgr5-positive cells proliferated even without supplement of EGF and FGF (FIGS. 9 and 23). This finding shows that large intestine CSCs have an intrinsic/autocrine mechanism for activating the Wnt signaling for their proliferation.

[0365] One of CSC's properties is resistance to chemotherapeutic agents. Thus, the present inventors tested large intestine CSCs for the sensitivity to 5-FU and irinotecan. As described above, the Lgr5-positive cells proliferated with a doubling time of about 2.5 days. Meanwhile, the Lgr5-negative CSCs were in a quiescent state in terms of growth. Both 5-FU (10 μg/ml) and irinotecan (10 μg/ml) treatments significantly inhibited the proliferation of Lgr5-positive large intestine CSCs, while they did not affect the proliferation and survival of Lgr5-negative large intestine CSCs (FIGS. 10 and 24). Three-day exposure of the Lgr5-positive large intestine CSCs to 5-FU (10 μg/ml) or irinotecan (10 μg/ml) caused the appearance of cells resistant to the chemotherapeutic agents. Surprisingly, the drug-resistant cells were negative for Lgr5 and had changed in morphology (FIGS. 11, 32, and 25). This finding demonstrates the transition from the Lgr5-positive to Lgr5-negative state.

[0366] HLA-DMA, TMEM173, ZMAT3, and GPR110 were chosen as markers for use in specific detection of such CSCs stably negative for Lgr5. Immunostaining performed using specific antibodies against the above molecules yielded a specific staining pattern with colon CSCs that converted to negative for Lgr5 after three days of irinotecan exposure (FIG. 43). Furthermore, this immunostaining method was demonstrated to be applicable to tissue sections prepared from paraffin blocks, which are used commonly (FIG. 43). These findings suggest that HLA-DMA, TMEM173, ZMAT3, GPR110 can serve as specific markers for CSCs that converted to negative for Lgr5.

[0367] The fluorescence representing Lgr5 positivity (FIG. 44A), which had been observed before irinotecan treatment, disappeared after the treatment (FIG. 44B). From Lgr5-negative cells again inoculated and cultured in the absence of irinotecan, Lgr5-positive cells appeared four days after the inoculation (FIG. 44C), and expanded by eight days after the inoculation (FIG. 44D). All the Lgr5-negative drug-resistant cells are negative for Lgr5 (FIGS. 44 and 45) and remained negative for CK20 (FIG. 46). This suggests that the transition of colon CSCs from the actively proliferating state to a quiescent state is correlated with the disappearance of Lgr5 molecule. The correlation was also verified by in vitro growth inhibitor-resistance assay (FIG. 47). In addition, the ALDH activity was reduced, while there was no alteration in other CSC markers (FIG. 48).

[0368] The Lgr5-negative cells prepared via irinotecan treatment were assessed for the tumor-forming activity. Subcutaneous injection of ten cells derived from PLR59 and PLR123 resulted in formation of tumors in two and one NOG mice (Table 4), respectively. Table 4 shows the tumor-forming activity of Lgr5-negative CSCs 49 days after inoculation. In Table 4 shown below, asterisk indicates tumor xenografts established in NOG mice; plus symbol (single) indicates the number of animals bearing tumors, and plus symbol (double) indicates the total number of animals.

	TABLE 4
		Number of cells/
	Cell	inoculation site
	line*	1,000	100	10
	PLR59	6+/6‡	6/6	2/6
	PLR123	6/6	6/6	1/6

[0369] In addition, to assess whether Lgr5-negative large intestine CSCs undergo a transition into an Lgr5-positive state, the present inventors cultured adherently Lgr5-negative large intestine CSCs prepared via irinotecan treatment again in a serum-free stem cell culture medium. The cells became positive for Lgr5 and exhibited mesenchymal cell-like morphology (FIGS. 12 and 33), and started to proliferate. On the other hand, when Lgr5-positive adherent large intestine CSCs were cultured in an ultra-low adherent plate, the present inventors observed that some of the cells halted their growth and formed a spheroid-like structure and that the Lgr5 mRNA level was very low (FIGS. 12 and 33). The transition from the Lgr5-positive to Lgr5-negative state (and the reverse) was also confirmed by observations using a single cell in culture. When single Lgr5-positive cells were individually cultured in a multi-well plate, the cells underwent a transition into the Lgr5-negative state within three days after irinotecan treatment. When single Lgr5-negative cells prepared via irinotecan treatment were individually cultured in a multi-well plate in the absence of irinotecan, 19 to 43% of the cells underwent a transition into the Lgr5-positive state within four days (FIG. 49 and Table 5).

	TABLE 5
	Cell	Number of cells
Transition of state	line	Lgr5 positive	Lgr5 negative	Total
Lgr5 positive to negative	PLR59	0	132	132
		(0%)	(100%)
	PLR123	1	173	174
		(1%)	(99%)
Lgr5 negative to positive	PLR59	18	78	96
		(19%)	(81%)
	PLR123	29	39	68
		(43%)	(57%)

[0370] Table 5 shows cell count ratios of Lgr5-positive and -negative cells stained by immunocytochemistry using an anti-Lgr5 antibody (antibody 2L36). Number in parenthesis represents the ratio of Lgr5-positive or -negative cell count.

[0371] Thus, the present inventors concluded that large intestine CSCs underwent interconversion between the Lgr5-positive and -negative states and the transition does not require any exogenous factor and/or niche environment.

[0372] Mesenchymal-like cells expressing nuclear β-catenin are considered migratory CSCs and metastatic CSCs that undergo EMT (Brabletz T, Jung A, Spaderna S, Hlubek F, Kirchner T (2005) Opinion: migrating cancer stem cells—an integrated concept of malignant tumor progression. Nat Rev Cancer 5:744-749). Since the morphology of Lgr5-positive large intestine CSCs was similar to that of mesenchymal cells, the present inventors tested whether Lgr5-positive large intestine CSCs correspond to migratory CSCs. Western blot analysis revealed low level expression of cell-surface E-cadherin, high level expression of Snail, and nuclear β-catenin (which is characteristic of EMT) in the Lgr5-positive large intestine CSCs (FIGS. 13, and 14, and 26). In contrast, the Lgr5-negative colorectal CSCs did not show any evidence of EMT. Specifically, cell-surface E-cadherin was expressed at a high level; Snail was expressed at a low level, and there was no nuclear localization of β-catenin. Furthermore, concomitant expression of Snail and Lgr5 was observed in cells that underwent EMT in budding areas of xenograft tumor tissues (FIG. 15). This finding supports the view that the Lgr5-positive large intestine CSCs correspond to migratory stem cells.

[0373] In addition, the present inventors demonstrated that the Lgr5-positive large intestine CSCs formed tumors in multiple tissues including lung, liver, lymph node, and subcutaneous tissues. Interestingly, in the liver, lymph node, and subcutaneous tissues, tumors with epithelial ductal structures were reconstituted by at least 40 days after intravenous injection of tumor cells, whereas such structures were not reconstituted in the lung (FIGS. 34 and 35).

[0374] Next, the present inventors examined whether Lgr5-negative CSCs directly form the hierarchical organization of cancer or first undergo transition to Lgr5-positive cells in vivo. To find markers for detecting Lgr5-negative CSCs, gene expression profiling was carried out using Lgr5-positive cells, Lgr5-negative cells, and primary cells from xenograft tumors. As a result, HLA-DMA was selected from molecules whose expression can be detected at high level in the Lgr5-negative CSCs as compared to the Lgr5-positive CSC and primary cells (FIG. 50). By immunohistochemistry using anti-Lgr5 antibody, anti-HLA-DMA antibody, and anti-EREG antibody, HLA-DMA was demonstrated to be specifically expressed in the Lgr5-negative CSCs (FIG. 51). HLA-DMA is also expressed in macrophages. Then, to rule out the possibility that cells stained by immunohistochemistry using the anti-HLA-DMA antibody are macrophages, the present inventors tested not only HLA-DMA but also other markers expressed in CSCs. Immunohistochemistry using an antibody against EREG expressed in both Lgr5-positive and -negative CSCs (FIG. 50) confirmed that EREG was expressed in both of Lgr5-positive and Lgr5-negative CSCs (FIG. 51). It was demonstrated that Lgr5-negative CSCs could be identified as cells that are positive for both HLA-DMA and EREG by detection using both markers in combination. After injection of a homogeneous population of Lgr5-negative CSCs to NOG mice, cells expressing Lgr5 only weakly for one day after the injection, which however remained positive for HLA-DMA and EREG, appeared. Then, cells that are negative for HLA-DMA but remain positive for Lgr5 and EREG appeared by five days after the injection (FIG. 52). Tumors derived from Lgr5-negative CSCs had specific ductal structures and included Lgr5-positive cells (FIG. 53).

[0375] To probe the possibility of in vivo transition to a growth inhibitor-resistant state, irinotecan was administered at the maximum tolerated dose (MTD) (120 mg/kg) into the peritoneal cavities of NOG mice bearing tumors derived from Lgr5-positive CSCs. Tumor growth was inhibited almost completely (FIG. 55), and the ductal structures were collapsed extensively (FIG. 54). This condition resulted in a dramatic decrease of Lgr5-positive cells (FIGS. 54 and 56). The number of Lgr5-negative and HLA-DMA-positive cells increased significantly after irinotecan treatment. By contrast, in vehicle-treated control mice, about one third of cancer cells were positive for Lgr5 in both ductal and budding areas (FIG. 54). Both Lgr5-positive cells and HLA-DMA-positive and Lgr5-negative cells were positive for EREG, and were identified to be CSCs (FIG. 54). After irinotecan treatment, Lgr5-positive cells appeared again (FIG. 54). The results described above, when considered together, suggest that Lgr5-negative CSCs can be the origin of colorectal cancer after growth inhibitor treatment and reconstitute cancer hierarchy via transition to Lgr5-positive cells.

[0376] Using a stem cell medium, Lgr5-positive adherent cells were seeded at 3×10⁵ cells/well in a 6-well plate (BD, Cat. No. 353046). On the following day, irinotecan (Hospira, 61703-349-09) was added to cells at a final concentration of 10 μg/ml. After three-day culture, irinotecan-resistant cells were detected. The cells were harvested using Accutase, and suspended in FACS buffer. Then, the cells were incubated at 4° C. for 30 minutes with 7-AAD Viability Dye as dead cell staining and each of the following antibodies as cancer stem cell markers:

[0377] FITC-labeled mouse mAb to human CD326 (EpCAM), PE-labeled mouse mAb to human CD133/1 (AC133), PE-labeled mouse mAb to human CD44, PE-labeled mouse mAb to human CD166, PE-labeled mouse mAb to human CD24, PE-labeled mouse mAb to human CD26, or PE-labeled mouse mAb to human CD29. To detect Lgr5, the cells were incubated with the mouse mAb to human Lgr5 at 4° C. for 30 minutes. After washing once with FACS buffer, the cells were incubated with the PE-labeled goat Ab to mouse IgG2a at 4° C. for 30 minutes. Then, after washing once with FACS buffer, the cells were subjected to flow cytometry analysis. The ALDH activity was detected using AldeFluor Kit according to the procedure recommended by the manufacturer. Flow cytometry analysis was performed using EPICS ALTRA. Cells negative for 7-AAD Viability Dye were analyzed for cancer stem cell markers. The irinotecan-resistant cells were demonstrated to change from positive to negative for Lgr5.

[0378] Primary cells from PLR59 and PLR123, high proliferative Lgr5-positive cancer stem cells prepared by adherent culture of primary cells, and low proliferative Lgr5-negative cancer stem cells prepared by irinotecan treatment of the cells as described above were homogenized mechanically with QIAshredder (Qiagen, Cat. No. 79654), and RNAs were extracted from them using RNeasy Mini Kit (Qiagen, Cat. No. 74104) and RNase-Free DNase Set (Qiagen, Cat. No. 79254) according to the procedure recommended by the manufacturer. The extracted RNAs were analyzed for the purity and quality using Agilent 2100 Bioanalyzer. Following cRNA synthesis, gene expression information was obtained using GeneChip (HG-U133 plus2) of Affymetrix. Data analysis was performed with Microsoft Excel and Statistics software R. The three types of cells (primary cells, Lgr5-positive cells, and Lgr5-negative cells) were compared to each other to make a list of genes whose expression levels are significantly increased in each cell type. Specifically, raw data from GeneChip were normalized and log 2 transformed by GCRMA to calculate differences in the expression level between distinct sample types (three types: primary cells and Lgr5-positive cells, Lgr5-positive cells and Lgr5-negative cells, and Lgr5-negative cells and primary cells). The criteria used for selecting differently expressed genes were:

(1) genes showing a twofold or more change in Lgr5-positive cells as compared to primary cells and a twofold or more change in Lgr5-negative cells as compared to primary cells (expressed at high levels in both Lgr5-positive and -negative cancer stem cells) (Table 6-1 to 6-10) (partial amino acid sequences of proteins encoded by the genes are shown in SEQ ID NOs: 1 to 6, and 9);
(2) genes showing a twofold or more change in Lgr5-positive cells as compared to primary cells and a less than twofold change in Lgr5-negative cells as compared to primary cells and (expressed at a high level in Lgr5-positive cancer stem cells alone) (Table 7-1 to 7-5);
(3) genes showing a less than twofold change in Lgr5-positive cells as compared to primary cells and a twofold or more change in Lgr5-negative cells as compared to primary cells (expressed at a high level in Lgr5-negative cancer stem cells alone) (Table 8-1 and 8-2) (partial amino acid sequences of proteins encoded by the genes are shown in SEQ ID NOs: 7 and 8).

[0379] Furthermore, to identify genes encoding proteins that are presented specifically on cell membrane of cancer stem cells, genes of GO:0005886 [plasma membrane] were extracted from GeneOntology (GO). Then, the present inventors extracted genes with GO:0005576 [extracellular region], GO:0009986 [cell surface], and GO:0016020 [membrane], or genes which are predicted to have a transmembrane region by membrane protein prediction software TMHMM and to have a signal peptide by signal peptide prediction software SignalP, and which do not have GO:0031090 [organelle membrane]. Furthermore, with the aid of GeneChip data from normal colorectal tissues, the present inventors exclude genes whose expression levels are relatively high in normal tissues or primary cells as well as gene only showing a small fold-change in Lgr5-positive or Lgr5-negative cells.

TABLE 6-1
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession	Abbre-		primary	primary	primary	primary-	ID
NO.	viation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO.
NP_036438.2	FAIM2	Fas apoptotic inhibitory molecule 2	8.4	7.0	5.4	4.1	—
NP_116265.1	JUB	jub, ajuba homolog (Xenopus laevis)	5.8	5.7	6.2	5.5	—
NP_116281.2	FRMD5	FERM domain containing 5	4.0	6.7	2.5	4.8	—
NP_071731.1	EDAR	ectodysplasin A receptor	7.0	7.6	5.3	4.6	1
NP_001240622.1	MCOLN3	mucolipin 3	3.7	5.1	0.8	2.5	—
NP_001007098.1	NTRK2	neurotrophic tyrosine kinase, receptor, type 2	3.6	5.2	1.0	2.6	—
NP_001243.1	CD70	CD70 molecule	4.0	4.9	4.1	4.7	2
NP_062818.1	SLCO1B3	solute carrier organic anion transporter family,	4.9	4.4	3.0	3.9	—
		member 1B3
NP_003606.3	SLC4A7	solute carrier family 4, sodium bicarbonate	2.9	4.0	1.6	3.2	—
		cotransporter, member 7
NP_005836.2	ABCC4	ATP-binding cassette, sub-family C (CFTR/MRP),	4.0	4.0	1.8	2.4	—
		member 4
NP_003920.1	RAB7L1	RAB7, member RAS oncogene family-like 1	5.5	5.3	5.6	6.0	—
NP_009162.1	SLC6A14	solute carrier family 6 (amino acid transporter),	1.9	3.7	4.7	6.3	—
		member 14
NP_872631.1	EFNA4	ephrin-A4	2.6	3.6	1.4	2.5	—
NP_001423.1	EREG	epiregulin	2.5	3.3	2.6	3.1	3
NP_001127839.1	SLC6A6	solute carrier family 6 (neurotransmitter transporter,	3.3	3.3	0.8	1.6	—
		taurine), member 6
NP_003497.2	FZD6	frizzled homolog 6 (Drosophila)	3.7	3.2	4.6	4.0	—
NP_003264.2	TM7SF2	transmembrane 7 superfamily member 2	4.0	4.2	6.8	6.2	—
NP_001172024.1	AIF1L	allograft inflammatory factor 1-like	3.5	2.9	2.3	2.0	—
NP_060033.3	IL17RD	interleukin 17 receptor D	3.1	2.8	2.4	3.0	—
NP_000013.2	ADA	adenosine deaminase	2.1	2.7	2.8	2.9	—
NP_004834.1	IL27RA	interleukin 27 receptor, alpha	3.6	2.6	2.2	1.8	—

[0380] Table 6-2 is a continuation of Table 6-1.

TABLE 6-2
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession	Abbre-		primary	primary	primary	primary	ID
NO.	viation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_001731.2	CALB2	calbindin 2	3.8	2.5	2.4	0.5	—
NP_065209.2	ANK1	ankyrin 1, erythrocytic	5.8	2.5	4.9	2.4	—
NP_001157412.1	PYGL	phosphorylase, glycogen, liver	1.2	2.5	1.7	2.1	—
NP_001097.2	ACVR2B	activin A receptor, type IIB	2.4	2.5	1.5	1.2	—
NP_001129141.1	XPR1	xenotropic and polytropic retrovirus receptor 1	3.4	2.5	3.5	2.4	—
NP_001523.2	SLC29A2	solute carrier family 29 (nucleoside transporters),	1.6	2.4	0.5	1.7	—
		member 2
NP_563615.3	DCBLD2	discoidin, CUB and LCCL domain containing 2	2.9	5.3	5.2	4.9	—
NP_689522.2	PIK3AP1	phosphoinositide-3-kinase adaptor protein 1	3.5	2.4	1.3	1.0	—
NP_054772.1	FLVCR1	feline leukemia virus subgroup C cellular receptor 1	3.5	2.4	1.7	1.2	—
NP_006849.1	TMED1	transmembrane emp24 protein transport domain	2.6	2.3	2.4	1.9	—
		containing 1
NP_116254.4	TNS4	tensin 4	1.9	2.3	1.9	1.5	—
NP_001193874.1	CSPG5	chondroitin sulfate proteoglycan 5 (neuroglycan C)	3.9	2.2	1.5	0.5	—
NP_000667.1	ADORA2B	adenosine A2b receptor	1.5	2.1	0.7	1.5	—
NP_064423.2	ACCN2	amiloride-sensitive cation channel 2, neuronal	1.8	1.9	1.7	1.3	—
NP_001018000.1	KAZ	kazrin	2.5	1.8	2.0	1.8	—
NP_004773.1	SNAP29	synaptosomal-associated protein, 29kDa	0.3	1.7	0.8	1.9	—
NP_066292.2	KCNJ12	potassium inwardly-rectifying channel, subfamily	3.4	3.5	4.0	3.4	—
		J, member 12
NP_938205.1	FLRT3	fibronectin leucine rich transmembrane protein 3	2.8	1.5	7.1	5.0	—
NP_115899.1	PARD6G	par-6 partitioning defective 6 homolog gamma	1.8	1.0	2.7	1.9	—
		(C. elegans)
NP_066924.1	CLDN1	claudin 1	1.4	0.7	3.4	3.5	—
NP_066939.1	ADCY1	adenylate cyclase 1 (brain)	2.9	2.2	2.1	1.4	—

[0381] Table 6-3 is a continuation of Table 6-2.

TABLE 6-3
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession	Abbre-		primary	primary	primary	primary	ID
NO.	viation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_001127807.1	AIMP2	aminoacyl tRNA synthetase complex-	1.0	0.6	1.2	0.7	—
		interacting multifunctional protein 2
NP_619539.1	AKAP7	A kinase (PRKA) anchor protein 7	2.0	1.3	1.4	1.2	—
NP_064715.1	ANKMY2	ankyrin repeat and MYND domain containing 2	2.7	2.5	3.2	3.4	—
NP_112591.2	APH1B	anterior pharynx defective 1	0.8	1.6	1.1	3.1	—
		homolog B (C. elegans)
NP_658985.2	APOA1BP	apolipoprotein A-I binding protein	1.8	1.3	2.2	1.6	—
NP_940852.3	APOOL	apolipoprotein O-like	1.6	1.6	2.3	2.3	—
NP_647537.1	ATRN	attractin	1.5	2.1	1.1	1.8	—
NP_001193.2	BMP4	bone morphogenetic protein 4	2.9	4.2	1.9	2.8	9
NP_001720.1	BTC	betacellulin	1.4	2.5	1.7	3.2	—
NP_001224.1	CAV2	caveolin 2	1.9	2.9	3.7	4.2	—
NP_001788.2	CDH11	cadherin 11, type 2, OB-cadherin (osteoblast)	6.2	2.4	3.8	0.6	—
NP_857592.1	CKLF	chemokine-like factor	0.9	1.6	-0.8	1.1	—
NP_612419.1	CMTM7	CKLF-like MARVEL transmembrane	2.8	4.2	2.3	3.2	—
		domain containing 7
NP_054860.1	CNTNAP2	contactin associated protein-like 2	4.6	4.3	3.8	3.7	—
NP_004738.3	DLG5	discs, large homolog 5 (Drosophila)	1.5	1.0	1.5	1.2	—
NP_001926.2	DPP4	dipeptidyl-peptidase 4	0.1	1.3	2.0	3.8	—
NP_690611.1	FAS	Fas (TNF receptor superfamily, member 6)	1.4	0.1	3.4	2.1	—
NP_001099043.1	FBXO45	F-box protein 45	1.0	1.3	0.3	1.2	—
NP_001138390.1	FGFR2	fibroblast growth factor receptor 2	3.9	1.7	3.0	1.0	—
NP_001457.1	FZD2	frizzled homolog 2 (Drosophila)	5.2	5.4	2.6	2.9	—
NP_031379.2	GNA12	guanine nucleotide binding protein	1.2	0.5	1.1	0.3	—
		(G protein) alpha 12

[0382] Table 6-4 is a continuation of Table 6-3.

TABLE 6-4
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession	Abbre-		primary	primary	primary	primary	ID
NO.	viation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_001243343.1	GNAI1	guanine nucleotide binding protein (G protein),	4.0	4.6	7.6	8.2	—
		alpha inhibiting activity polypeptide 1
NP_002766.1	HTRA1	HtrA serine peptidase 1	5.1	7.0	5.0	6.3	—
NP_001543.2	IGFBP4	insulin-like growth factor binding protein 4	3.4	2.7	0.5	1.1	—
NP_001002915.2	IGFL2	IGF-like family member 2	3.0	3.3	2.3	2.5	—
NP_001034659.2	KREMEN1	kringle containing transmembrane protein 1	3.0	3.7	1.3	2.3	—
NP_005597.3	LGMN	legumain	1.1	1.8	0.5	1.4	—
NP_002303.2	LIG4	ligase IV, DNA, ATP-dependent	3.7	3.7	5.6	5.4	—
NP_006024.1	LIPG	lipase, endothelial	2.1	2.6	0.1	1.1	—
NP_001392.2	LPAR1	lysophosphatidic acid receptor 1	2.9	3.4	1.3	2.4	—
NP_036284.1	LPAR3	lysophosphatidic acid receptor 3	6.7	4.9	3.7	2.9	—
NP_002327.2	LRP6	low density lipoprotein receptor-related protein 6	2.8	1.8	1.5	0.6	—
NP_055414.2	MAGED2	melanoma antigen family D, 2	1.1	1.8	2.7	2.9	—
NP_005922.2	MICB	MHC class I polypeptide-related sequence B	2.6	3.6	2.3	3.7	—
NP_001182555.1	MLLT10	myeloid/lymphoid or mixed-lineage leukemia	2.5	2.0	1.0	1.3	—
		(trithorax homolog, Drosophila); translocated to,
NP_002435.1	MSN	10 moesin	3.0	1.7	2.2	0.0	—
NP_001018169.1	NAE1	NEDD8 activating enzyme El subunit 1	1.8	1.7	1.2	1.8	—
NP_056146.1	NCSTN	nicastrin	1.5	0.4	2.3	0.8	—
NP_060562.3	NETO2	neuropilin (NRP) and tolloid (TLL)-like 2	7.3	6.1	8.0	6.9	—
NP_009014.2	NUDT6	nudix (nucleoside diphosphate linked moiety X)-	3.2	1.9	4.2	2.9	—
		type motif 6
NP_689501.1	ORAI3	ORAI calcium release-activated calcium modulator 3	1.5	1.1	4.3	2.2	—
NP_002605.2	PDZK1	PDZ domain containing 1	8.1	8.3	4.9	3.6	—

[0383] Table 6-5 is a continuation of Table 6-4.

TABLE 6-5
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession	Abbre-		primary	primary	primary	primary	ID
NO.	viation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_055824.1	PDZRN3	PDZ domain containing ring finger 3	2.6	2.5	1.4	0.6	—
NP_077734.1	PLA2G4A	phospholipase A2, group IVA (cytosolic,	7.7	8.2	5.0	5.9	—
		calcium-dependent)
NP_001123508.1	PLEKHB1	pleckstrin homology domain containing, family B	4.0	5.3	5.1	6.3	—
		(evectins) member 1
NP_001123508.1	PNPLA3	patatin-like phospholipase domain containing 3	4.4	4.9	1.7	3.5	—
NP_004641.1	PNPLA4	patatin-like phospholipase domain containing 4	2.3	1.9	2.8	2.2	—
NP_006395.2	PROCR	protein C receptor, endothelial	1.3	1.5	4.2	3.6	4
NP_001159449.1	PROM2	prominin 2	5.4	4.6	10.5	10.4	5
NP_077748.3	PSTPIP2	proline-serine-threonine phosphatase	2.2	2.7	5.1	4.9	—
		interacting protein 2
NP_002834.3	PTPRJ	protein tyrosine phosphatase, receptor type, J	2.9	3.0	1.5	2.2	—
NP_002861.1	RAB13	RAB13, member RAS oncogene family	1.3	0.9	2.0	1.4	—
NP_066361.1	RAP2A	RAP2A, member of RAS oncogene family	2.0	1.7	2.4	1.7	—
NP_001094058.1	RC3H2	ring finger and CCCH-type zinc finger domains 2	1.2	0.3	2.0	0.0	—
NP_002897.1	RDX	radixin	4.7	5.3	4.5	4.6	—
NP _006502.1	RSC1A1	regulatory solute carrier protein, family 1, member 1	1.1	0.0	1.2	0.4	—
	(DDI2)	(DNA-damage inducible 1 homolog 2)
NP_004162.2	SLC1A2	solute carrier family 1 (glial high affinity glutamate	7.4	7.3	2.1	1.9	—
		transporter), member 2
NP_006349.1	SLC25A17	solute carrier family 25 (mitochondrial carrier;	2.4	1.9	2.4	1.5	—
		peroxisomal membrane protein, 34kDa), member 17
NP_075053.2	SLC30A5	solute carrier family 30 (zinc transporter), member 5	1.3	0.9	1.2	0.8	—
NP_001070253.1	SLC7A6	solute carrier family 7 (cationic amino	1.0	0.2	1.5	0.8	—
		acid transporter,
		y+system), member 6
NP_071420.1	SMOC1	SPARC related modular calcium binding 1	2.6	5.4	−0.1	2.4	—
NP_001159884.1	SMOC2	SPARC related modular calcium binding 2	7.2	8.4	−0.9	2.2	—
NP_054730.1	SOCS5	suppressor of cytokine signaling 5	2.3	1.7	2.5	1.9	—

[0384] Table 6-6 is a continuation of Table 6-5.

TABLE 6-6
DB			PLR59	PLR123	PLR59	PLR123	SEQ
accession	Abbre-		primary	primary	primary	primary	ID
NO.	viation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_001030127.1	SORBS1	sorbin and SH3 domain containing 1	6.4	4.3	1.1	−0.2	—
NP_003095.2	SORD	sorbitol dehydrogenase	1.5	1.8	1.0	2.1	—
NP_003705.1	STC2	stanniocalcin 2	3.6	3.1	1.2	2.3	—
NP_005810.1	STX6	syntaxin 6	3.4	2.5	4.4	3.2	—
NP_003229.1	TGFB2	transforming growth factor, beta 2	5.3	5.1	4.2	3.6	—
NP_001124388.1	TGFBR1	transforming growth factor, beta receptor 1	1.9	1.8	1.5	1.0	—
NP_057635.1	TM7SF3	transmembrane 7 superfamily member 3	1.9	1.6	2.7	2.2	—
NP_653233.3	TMEM182	transmembrane protein 182	3.3	3.4	4.6	4.6	—
NP_003802.1	TNFSF9	tumor necrosis factor (ligand)	4.5	4.4	5.2	4.9	6
		superfamily, member 9
NP_005714.2	TSPAN5	tetraspanin 5	1.8	2.9	0.0	1.5	—
NP_068835.1	UTS2	urotensin 2	2.4	1.7	2.5	2.3	—

[0385] Table 6-7 is a continuation of Table 6-6.

TABLE 6-7
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
No.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_056222.2	ABHD14A	abhydrolase domain containing 14A	1.3	0.9	2.7	1.9	—
NP_004449.1	ACSL4	acyl-CoA synthetase long-chain family member 4	4.5	4.5	3.6	4.2	—
NP_665812.1	AIFM1	apoptosis-inducing factor, mitochondrion-associated, 1	1.9	1.6	2.0	1.9	—
NP_001344.2	AKR1C1	aldo-keto reductase family 1, member C1 (dihydrodiol	9.4	7.8	8.1	2.7	—
		dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid
		dehydrogenase)
NP_940683.1	ANKRD46	ankyrin repeat domain 46	2.6	1.8	3.5	2.2	—
NP_077027.1	APOO	apolipoprotein O	1.4	1.5	1.2	1.0	—
NP_004766.2	B4GALT6	UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase,	2.0	1.5	0.3	1.1	—
		polypeptide 6
NP_004326.1	BST2	bone marrow stromal cell antigen 2	−3.9	4.4	−3.9	5.9	—
NP_001185983.1	C16orf5	chromosome 16 open reading frame 5	3.1	2.6	6.9	5.4	—
NP_115700.1	C1orf57	chromosome 1 open reading frame 57	1.5	1.3	3.0	3.0	—
NP_653181.1	C1orf85	chromosome 1 open reading frame 85	1.1	0.8	2.9	2.4	—
NP_001074293.1	C2orf89	chromosome 2 open reading frame 89	1.9	1.7	0.8	1.1	—
NP_775823.1	C3orf58	chromosome 3 open reading frame 58	2.0	0.3	1.6	−0.4	—
NP_439896.1	C6orf192	chromosome 6 open reading frame 192	4.0	2.5	4.5	3.3	—
NP_001135942.1	C6orf203	chromosome 6 open reading frame 203	1.2	1.0	2.0	1.8	—
NP_620140.1	C6orf72	chromosome 6 open reading frame 72	1.3	1.8	1.2	1.7	—
NP_001243894.1	CCDC51	coiled-coil domain containing 51	1.1	0.9	3.6	3.1	—
NP_001157882.1	CDK5	cyclin-dependent kinase 5	1.9	2.0	3.5	3.3	—
NP_055061.1	CELSR1	cadherin, EGF LAG seven-pass G-type receptor 1	0.5	1.5	0.6	1.1	—
		(flamingo homolog, Drosophila)
NP_004077.1	COCH	coagulation factor C homolog, cochlin (Limulus	2.1	2.3	0.5	2.2	—
		polyphemus)
NP_001896.2	CTPS	CTP synthase	2.0	1.0	1.4	0.7	—
NP_055191.2	CYFIP2	cytoplasmic FMR1 interacting protein 2	2.6	3.9	5.0	5.3	—
NP_004393.1	DFFB	DNA fragmentation factor, 40 kDa, beta polypeptide	2.3	1.8	1.7	1.5	—
		(caspase-activated DNase)
NP_001077058.1	E2F5	E2F transcription factor 5, p130-binding	2.8	2.2	2.7	2.2	—

[0386] Table 6-8 is a continuation of Table 6-7.

TABLE 6-8
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_056067.2	EHBP1	EH domain binding protein 1	2.3	1.9	1.1	0.4	—
NP_060682.2	ENAH	enabled homolog (Drosophila)	4.4	3.5	1.5	1.2	—
NP_001180289.1	EXD2	exonuclease 3′-5′ domain containing 2	2.3	1.2	2.7	1.9	—
NP_660323.3	FAM119A	family with sequence similarity 119, member A	5.4	5.1	3.0	3.7	—
NP_620775.2	FAM175A	family with sequence similarity 175, member A	3.4	2.8	3.8	2.8	—
NP_937995.1	FAM189B	family with sequence similarity 189, member B	1.7	1.7	1.3	1.1	—
NP_055679.1	FAM20B	family with sequence similarity 20, member B	1.6	0.8	1.5	0.8	—
NP_942600.1	FIBP	fibroblast growth factor (acidic) intracellular binding	1.0	1.2	2.0	1.7	—
		protein
NP_000139.1	FUT1	fucosyltransferase 1 (galactoside 2-alpha-L-	7.0	5.6	3.1	4.0	—
		fucosyltransferase, H blood group)
NP_000135.2	FXN	frataxin	1.7	1.1	2.0	1.6	—
NP_000393.4	G6PD	glucose-6-phosphate dehydrogenase	0.8	1.0	2.0	1.4	—
NP_000143.2	GAA	glucosidase, alpha; acid	1.2	1.4	3.3	2.2	—
NP_000160.1	GLA	galactosidase, alpha	2.0	1.1	1.5	0.9	—
NP_002072.2	GPC1	glypican 1	1.0	1.2	2.6	0.5	—
NP_001008398.2	GPX8	glutathione peroxidase 8 (putative)	5.0	5.5	7.1	7.0	—
NP_005329.3	HIP1	huntingtin interacting protein 1	2.6	2.7	2.6	2.5	—
NP_254274.1	IL33	interleukin 33	−0.5	1.2	3.6	3.4	—
NP_002262.3	IPO5	importin 5	3.6	2.3	1.9	0.4	—
NP_060573.2	LRRC8D	leucine rich repeat containing 8 family, member D	2.0	1.0	1.9	1.2	—
NP_067679.6	MFAP3L	microfibrillar-associated protein 3-like	1.5	0.2	3.3	1.2	—
NP_612440.1	MFSD3	major facilitator superfamily domain containing 3	0.9	1.3	2.0	2.4	—
NP_066014.1	MOV10	Mov10, Moloney leukemia virus 10, homolog (mouse)	0.5	1.4	1.3	2.0	—
NP_036351.3	MRAS	muscle RAS oncogene homolog	1.1	0.6	4.3	1.3	—
NP_057034.2	MRPL2	mitochondrial ribosomal protein L2	1.3	0.8	0.8	0.0	—
NP_065972.3	NIN	ninein (GSK3B interacting protein)	2.0	3.1	2.7	3.0	—

[0387] Table 6-9 is a continuation of Table 6-8.

TABLE 6-9
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_065181.1	NIPAL3	NIPA-like domain containing 3	1.7	1.3	3.0	2.3	—
NP_002504.2	NME3	non-metastatic cells 3, protein expressed in	1.1	2.1	1.5	1.9	—
NP_057672.1	NRN1	neuritin 1	8.5	5.7	7.7	6.3	—
NP_689643.2	OR51E1	olfactory receptor, family 51, subfamily E, member 1	6.9	9.8	0.3	3.0	—
NP_071748.2	OSGEPL1	O-sialoglycoprotein endopeptidase-like 1	4.1	4.7	3.1	4.4	—
NP_079431.1	PAAF1	proteasomal ATPase-associated factor 1	1.9	2.1	1.5	1.9	—
NP_000523.2	PCCB	propionyl CoA carboxylase, beta polypeptide	1.4	1.6	1.4	2.0	—
NP_061757.1	PCDHB14	protocadherin beta 14	1.4	0.5	1,4	0.1	—
NP_002622.2	PGD	phosphogluconate dehydrogenase	1.2	1.4	1.2	1.2	—
NP_003550.1	PIP4K2B	phosphatidylinositol-5-phosphate 4-kinase, type II, beta	1.8	1.3	1.8	1.1	—
NP_056530.2	PLA2G3	phospholipase A2, group III	2.6	4.3	0.1	3.2	—
NP_005038.1	PSMD5	proteasome (prosome, macropain) 26S subunit, non-	1.5	0.7	1.7	0.7	—
		ATPase, 5
NP_006255.1	PTPN13	protein tyrosine phosphatase, non-receptor type 13	0.1	1.1	0.7	2.2	—
		(APO-1/CD95 (Fas)-associated phosphatase)
NP_057161.1	PTRH2	peptidyl-tRNA hydrolase 2	1.4	1.4	1.5	1.8	—
NP_037390.2	PYCARD	PYD and CARD domain containing	0.2	1.3	1.1	2.1	—
NP_055113.2	QPRT	quinolinate phosphoribosyltransferase	2.5	3.6	3.1	4.5	—
NP_060233.3	RNF43	ring finger protein 43	2.7	2.7	1.2	1.5	—
NP_060616.1	RNMTL1	RNA methyltransferase like 1	1.5	0.9	1.7	1.1	—
NP_003698.1	RUVBL1	RuvB-like 1 (E. coli)	1.9	2.2	1.3	2.2	—
NP_002949.2	RYK	RYK receptor-like tyrosine kinase	1.5	1.0	1.2	1.2	—
NP_116250.3	SERAC1	serine active site containing 1	1.8	0.8	2.7	1.5	—
NP_005016.1	SERPINI1	serpin peptidase inhibitor, Glade I (neuroserpin), member 1	5.5	8.7	2.1	4.2	—
NP_008927.1	SLC19A2	solute carrier family 19 (thiamine transporter), member 2	3.8	2.7	2.0	1.2	—
NP_065075.1	SLC39A10	solute carrier family 39 (zinc transporter), member 10	2.9	3.1	2.6	2.7	—
NP_060306.3	SLC41A3	solute carrier family 41, member 3	1.9	1.5	1.3	0.8	—

[0388] Table 6-10 is a continuation of Table 6-9.

TABLE 6-10
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_005692.1	SNUPN	snurportin 1	2.2	2.4	2.3	2.5	—
NP_055563.1	SNX17	sorting nexin 17	0.8	1.1	1.0	1.0	—
NP_003156.1	STXBP1	syntaxin binding protein 1	4.3	5.7	4.9	5.3	—
NP_003192.1	TFAM	transcription factor A, mitochondrial	2.1	1.6	1.1	1.6	—
NP_444283.2	THEM4	thioesterase superfamily member 4	1.6	−0.5	2.8	0.4	—
NP_612472.1	TLCD1	TLC domain containing 1	2.7	2.1	3.9	4.2	—
NP_057548.1	TMEM138	transmembrane protein 138	1.8	2.2	2.0	2.7	—
NP_085054.2	TMEM177	transmembrane protein 177	3.1	3.2	3.5	4.1	—
NP_056236.2	TMEM186	transmembrane protein 186	2.4	2.0	2.0	2.3	—
NP_078863.2	TMEM53	transmembrane protein 53	1.2	1.3	1.2	1.0	—
NP_001008495.2	TMEM64	transmembrane protein 64	6.7	5.7	8.3	8.0	—
NP_689630.1	TMEM68	transmembrane protein 68	1.8	1.8	3.4	2.9	—
NP_861448.2	TMTC3	transmembrane and tetratricopeptide repeat containing 3	2.5	1.8	3.8	2.3	—
NP_775107.1	TRIM59	tripartite motif-containing 59	3.7	4.3	3.0	4.0	—
NP_003293.2	TRIP6	thyroid hormone receptor interactor 6	2.7	1.3	5.1	3.3	—
NP_009215.1	TWF2	twinfilin, actin-binding protein, homolog 2 (Drosophila)	0.6	1.5	1.4	1.8	—
NP_079094.1	UBA5	ubiquitin-like modifier activating enzyme 5	1.8	2.1	2.4	2.4	—
NP_060769.4	UBE2W	ubiquitin-conjugating enzyme E2W (putative)	1.3	1.7	2.4	2.4	—
NP_001017980.1	VMA21	VMA21 vacuolar H+-ATPase homolog (S. cerevisiae)	3.0	2.6	2.9	2.6	—
NP_660295.2	ZG16B	zymogen granule protein 16 homolog B (rat)	1.1	2.0	1.4	1.9	—
NP_115549.2	ZNRF3	zinc and ring finger 3	4.7	4.1	2.2	2.6	—

(The values in Tables 6-1 to 6-10 shown above represent the expression difference (log 2 ratio).)

TABLE 7-1
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_003658.1	LGR5	leucine-rich repeat-containing G protein-coupled	3.0	3.8	−1.5	0.4	—
		receptor 5
NP_066301.1	SNTB1	syntrophin, beta 1 (dystrophin-associated protein A1,	3.1	3.1	0.0	0.4	—
		59 kDa, basic component 1)
NP_001123575.1	COL13A1	collagen, type XIII, alpha 1	2.0	2.9	−0.2	0.3	—
NP_001167538.1	FGFR1	fibroblast growth factor receptor 1	1.3	2.3	−0.7	0.6	—
NP_004432.1	EPHB1	EPH receptor B1	1.0	2.3	−0.3	0.7	—
NP_002002.3	FGFR4	fibroblast growth factor receptor 4	2.0	2.2	−1.9	−0.5	—
NP_004622.2	LRP8	low density lipoprotein receptor-related protein 8,	2.3	2.1	0.5	0.7	—
		apolipoprotein e receptor
NP_002832.3	PTPRG	protein tyrosine phosphatase, receptor type, G	1.8	2.1	−5.0	−5.4	—
NP_004727.2	XPR1	xenotropic and polytropic retrovirus receptor 1	1.5	2.1	0.7	0.9	—
NP_859052.3	QSOX2	quiescin Q6 sulfhydryl oxidase 2	1.8	2.0	0.7	0.8	—
NP_003876.1	CDK5R1	cyclin-dependent kinase 5, regulatory subunit 1 (p35)	3.1	3.3	0.3	2.0	—
NP_002821.1	PTPN4	protein tyrosine phosphatase, non-receptor type 4	1.7	1.9	0.3	0.7	—
		(megakaryocyte)
NP_001457.1	FZD2	frizzled homolog 2 (Drosophila)	5.2	5.4	2.6	2.9	—
NP_000674.2	ADRA2C	adrenergic, alpha-2C-, receptor	1.8	1.6	0.1	−0.1	—
NP_000334.1	SLC5A1	solute carrier family 5 (sodium/glucose cotransporter),	2.2	1.6	0.2	0.6	—
		member 1
NP_005901.2	MAPT	microtubule-associated protein tau	1.9	1.5	0.4	0.6	—
NP_598328.1	SYN2	synapsin II	0.3	1.7	0.2	0.5	—
NP_005496.4	SCARB1	scavenger receptor class B, member 1	1.1	1.2	−0.3	−0.3	—
NP_004434.2	EPHB3	EPH receptor B3	0.7	1.0	−0.5	−0.4	—
NP_000259.1	NF2	neurofibromin 2 (merlin)	1.2	0.9	0.6	0.1	—
NP_003477.4	SLC7A5	solute carrier family 7 (cationic amino acid	2.0	0.8	−0.4	−0.7	—
		transporter, y + system), member 5
NP_054740.3	SSX2IP	synovial sarcoma, X breakpoint 2 interacting protein	1.8	0.7	0.9	−0.3	—
NP_055736.2	LPHN1	latrophilin 1	1.4	0.6	0.9	0.3	—
NP_004435.3	EPHB4	EPH receptor 84	1.3	0.5	0.7	−0.1	—

[0389] Table 7-2 is a continuation of Table 7-1.

TABLE 7-2
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_116197.4	LINGO1	leucine rich repeat and Ig domain containing 1	1.2	0.0	0.6	−1.3	—
NP_004987.2	ABCC1	ATP-binding cassette, sub-family C (CFTR/MRP),	1.1	0.9	0.0	−0.2	—
		member 1
NP_003174.3	ADAM17	ADAM metallopeptidase domain 17	1.0	0.3	−0.3	−0.4	—
NP_620686.1	ADAMTS15	ADAM metallopeptidase with thrombospondin type 1	6.1	4.6	−3.6	−4.2	—
		motif, 15
NP_005091.2	AKAP12	A kinase (PRKA) anchor protein 12	2.4	3.7	−1.2	0.3	—
NP_001618.2	ALCAM	activated leukocyte cell adhesion molecule	1.3	0.9	0.3	0.6	—
NP_001648.1	AREGB	amphiregulin B	1.7	0.4	0.0	−0.5	—
NP_001164.2	ARHGAP5	Rho GTPase activating protein 5	1.2	0.4	0.8	0.3	—
NP_542172.2	B3GALT6	UDP-Gal:betaGal beta 1,3-galactosyltransferase	1.1	0.6	0.6	0.3	—
		polypeptide 6
NP_001711.2	BMP8B	bone morphogenetic protein 8b	0.9	1.3	0.4	0.3	—
NP_006560.3	CGREF1	cell growth regulator with EF-hand domain 1	0.9	1.0	0.3	0.8	—
NP_058647.1	CKLF	chemokine-like factor	0.9	1.5	−0.5	0.9	—
NP_849199.2	CMTM8	CKLF-like MARVEL transmembrane domain containing 8	1.3	1.4	0.6	0.7	—
NP_001422.1	EPB41L2	erythrocyte membrane protein band 4.1-like 2	1.3	0.9	−0.4	−0.4	—
NP_004433.2	EPHB2	EPH receptor B2	1.0	0.5	−3.1	−2.8	—
NP_000496.2	F12	coagulation factor XII (Hageman factor)	1.2	1.6	0.2	1.0	—
NP_057133.2	FAM158A	family with sequence similarity 158, member A	0.8	1.2	0.1	0.4	—
NP_001990.2	FBN2	fibrillin 2	2.6	2.5	−0.4	0.1	—
NP_068656.21	FGG	fibrinogen gamma chain	1.3	0.6	0.6	0.5	—
	(OSMR)	(oncostatin M receptor)
NP_001439.2	GPC4	glypican 4	2.0	2.1	−0.9	0.0	—
NP_065857.1	GPHN	gephyrin	3.2	2.9	−3.4	−2.6	—
NP_057399.1	GULP1	GULP, engulfment adaptor PTB domain containing 1	1.4	2.0	0.4	0.9	—
NP_036616.2	HMMR	hyaluronan-mediated motility receptor (RHAMM)	5.0	4.5	−1.7	−1.5	—
NP_000866.1	IGF1R	insulin-like growth factor 1 receptor	0.7	1.1	−1.9	−1.2	—

[0390] Table 7-3 is a continuation of Table 7-2.

TABLE 7-3
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_000588.2	IGFBP2	insulin-like growth factor binding protein 2, 36 kDa	1.4	0.7	0.3	-0.4	—
NP_061195.2	IL17RB	interleukin 17 receptor B	1.0	1.2	−2.7	−1.9	—
NP_002326.2	LRP5	low density lipoprotein receptor-related protein 5	1.2	1.7	−0.8	−0.2	—
NP_006491.2|	MCAM	melanoma cell adhesion molecule	2.1	1.5	−0.2	−0.4	—
NP_055456.2	MDC1	mediator of DNA-damage checkpoint 1	2.6	2.2	−1.3	−1.7	—
NP_055606.1	MELK	maternal embryonic leucine zipper kinase	1.5	1.6	−2.0	−1.1	—
NP_000236.2	MET	met proto-oncogene (hepatocyte growth factor receptor)	1.2	1.2	0.6	0.4	—
NP_065825.1	MIB1	mindbomb homolog 1 (Drosophila)	1.3	1.3	0.7	0.5	—
NP_005952.2	MUC6	mucin 6, oligomeric mucus/gel-forming	1.1	3.0	−0.1	1.0	—
NP_777596.2	PCSK9	proprotein convertase subtilisin/kexin type 9	1.5	1.8	−1.6	−0.4	—
NP_003619.2	PKP4	plakophilin 4	1.5	1.4	0.0	−0.1	—
NP_003042.3	SLC16A1	solute carrier family 16, member 1	1.7	0.6	0.3	0.3	—
		(monocarboxylic acid transporter 1)
NP_057438.3	SLCO4A1	solute carrier organic anion transporter family,	0.5	1.2	−1.4	−0.2	—
		member 4A1
NP_003093.2	SOD3	superoxide dismutase 3, extracellular	2.2	1.7	0.6	−1.2	—
NP_006425.2	SORBS1	sorbin and SH3 domain containing 1	3.2	3.4	−0.9	0.2	—
NP_003095.2	SORD	sorbitol dehydrogenase	1.1	0.7	0.9	0.8	—
NP_000342.2	STS	steroid sulfatase (microsomal), isozyme S	1.8	2.2	−0.5	−0.7	—
NP_003234.2	TGFBR3	transforming growth factor, beta receptor III	1.4	1.8	−0.2	−0.2	—
NP_055388.2	TMEM97	transmembrane protein 97	2.6	2.1	1.0	−0.3	—
CAA26435.1	TRAC	T cell receptor alpha constant	0.0	1.1	0.0	0.0	—

[0391] Table 7-4 is a continuation of Table 7-3.

TABLE 7-4
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_076417.2	AACS	acetoacetyl-CoA synthetase	1.1	1.6	0.2	0.6	—
NP_653191.2	ANKRD22	ankyrin repeat domain 22	2.0	1.5	−1.8	−1.7	—
NP_056020.2	ATP11A	ATPase, class VI, type 11A	1.3	1.0	0.7	0.6	—
NP_001159.2	BIRC5	baculoviral IAP repeat-containing 5	2.7	2.4	−4.8	−4.4	—
NP_061189.2	CDCA7L	cell division cycle associated 7-like	2.1	1.8	−0.6	0.4	—
NP_005183.2	CDKN3	cyclin-dependent kinase inhibitor 3	4.8	5.3	−3.1	−1.8	—
NP_004077.1	COCH	coagulation factor C homolog, cochlin (Limulus	1.2	1.2	−0.4	0.7	—
		polyphemus)
NP_005302.3	GRB10	growth factor receptor-bound protein 10	1.1	0.4	−1.2	−1.5	—
NP_671704.3	HS6ST2	heparan sulfate 6-O-sulfotransferase 2	1.8	1.6	−1.1	−0.2	—
NP_002262.3	IPO5	importin 5	2.6	1.7	0.5	−0.3	—
NP_114428.1	ITFG3	integrin alpha FG-GAP repeat containing 3	1.0	0.9	0.0	−1.1	—
NP_002241.1	KCNN4	potassium intermediate/small conductance calcium-	1.2	0.9	−0.7	−0.7	—
		activated channel, subfamily N, member 4
NP_061159.1	KIAA1199	KIAA1199	1.1	1.1	−1.5	−2.4	—
NP_115940.2	KISS1R	KISS1 receptor	1.1	1.8	−3.0	0.2	—
NP_057034.2	MRPL2	mitochondrial ribosomal protein L2	1.3	0.8	0.8	0.0	—
NP_000242.1	MSH2	mutS homolog 2, colon cancer, nonpolyposis type 1	2.5	2.8	−0.8	−0.4	—
		(E. coli)
NP_055452.3	MTFR1	mitochondrial fission regulator 1	1.6	0.9	0.2	−0.1	—
NP_005947.3	MTHFD1	methylenetetrahydrofolate dehydrogenase (NADP+	1.7	1.6	0.3	0.1	—
		dependent) 1, methenyltetrahydrofolate cyclohydrolase,
		formyitetrahydrofolate synthetase
NP_005366.2	MYB	v-myb myeloblastosis viral oncogene homolog (avian)	2.7	2.3	−4.4	0.2	—
NP_078938.2	NAT10	N-acetyltransferase 10 (GCN5-related)	1.9	1.3	0.1	−0.1	—
NP_777549.1	NDUFAF2	NADH dehydrogenase (ubiquinone) 1 alpha	1.0	1.4	−0.9	0.1	—
		subcomplex, assembly factor 2
NP_004280.5	NFE2L3	nuclear factor (erythroid-derived 2)-like 3	1.2	1.2	−0.9	−0.1	—
NP_006672.1	NMU	neuromedin U	0.2	1.6	−4.7	0.2	—
NP_055950.1	NUP205	nucleoporin 205 kDa	1.9	1.7	−0.3	−0.2	—

[0392] Table 7-5 is a continuation of Table 7-4.

TABLE 7-5
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_942590.1	NUP43	nucleoporin 43 kDa	1.3	0.9	0.0	0.0	—
NP_055484.3	NUP93	nucleoporin 93 kDa	2.1	2.4	0.1	0.8	—
NP_006614.2	PHGDH	phosphoglycerate dehydrogenase	3.0	3.4	−2.0	−0.4	—
NP_060904.2	RNF130	ring finger protein 130	0.9	1.0	0.4	0.3	—
NP_060259.4	SEMA4C	sema domain, immunoglobulin domain (Ig),	1.9	2.3	0.2	0.5	—
		transmembrane domain (TM) and short
		cytoplasmic domain, (semaphorin) 4C
NP_005857.1	SIGMAR1	sigma non-opioid intracellular receptor 1	1.0	1.1	0.8	0.9	—
NP_055413.1	SOCS7	suppressor of cytokine signaling 7	1.0	0.6	0.7	0.1	—
NP_005554.1	STMN1	stathmin 1	1.9	2.1	−3.1	−1.4	—
NP_054897.4	STXBP6	syntaxin binding protein 6 (amisyn)	2.1	2.1	−3.4	−0.1	—
NP_001070884.1	TMEM231	transmembrane protein 231	0.5	1.5	−2.2	−0.8	—
NP_002537.3	TNFRSF11B	tumor necrosis factor receptor superfamily,	1.7	6.3	−2.9	−0.9	—
		member 11b
NP_443195.1	TOP1MT	topoisomerase (DNA) I, mitochondrial	1.1	0.4	0.5	−0.1	—
NP_001058.2	TOP2A	topoisomerase (DNA) II alpha 170 kDa	2.2	2.7	−6.1	−3.1	—
NP_006364.2	VAT1	vesicle amine transport protein 1 homolog	1.0	1.5	0.5	1.0	—
		(T. californica)
NP_612471.1	ZMYND19	zinc finger, MYND-type containing 19	1.2	0.7	0.7	0.3	—

(The values in Tables 7-1 to 7-5 shown above represent the expression difference (log 2 ratio).)

TABLE 8-1
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_076404.3	GPR87	G protein-coupled receptor 87	0.0	0.0	7.1	7.4	—
NP_005109.2	TNFSF15	tumor necrosis factor (ligand) superfamily, member 15	−2.4	−2.3	3.5	3.3	—
NP_001926.2	DPP4	dipeptidyl-peptidase 4	0.1	1.3	2.0	3.8	—
NP_149017.2	BBS4	Bardet-Biedl syndrome 4	0.2	0.6	1.7	2.6	—
NP_542386.1	C9orf30	chromosome 9 open reading frame 30	0.2	0.2	1.5	1.7	—
NP_001447.2	FLNA	filamin A, alpha	−0.4	0.8	0.7	1.7	—
NP_000425.1	NEU1	sialidase 1 (lysosomal sialidase)	0.3	0.4	1.9	1.7	—
NP_002125.3	HMOX2	heme oxygenase (decycling) 2	0.3	0.4	1.5	1.6	—
NP_001078.2	AAMP	angio-associated, migratory cell protein	0.1	0.3	1.2	1.6	—
NP_061985.2	ABCA7	ATP-binding cassette, sub-family A (ABC1), member 7	−1.3	0.4	2.1	1.6	—
NP_000401.1	HFE	hemochromatosis	2.2	2.0	1.2	2.8	—
NP_001142.2	SLC25A4	solute carrier family 25 (mitochondrial carrier: adenine	0.6	0.8	1.3	1.5	—
		nucleotide translocator), member 4
NP_006569.1	GNBS	guanine nucleotide binding protein (G protein), beta 5	0.2	0.8	0.9	1.4	—
NP_005846.1	RAMP1	receptor (G protein-coupled) activity modifying protein 1	−0.5	−0.5	1.1	1.4	—
NP_598378.3	RHOV	ras homolog gene family, member V	0.1	−0.2	0.5	1.4	—
NP_112178.2	PVRL4	poliovirus receptor-related 4	−0.3	−2.8	6.1	1.8	7
NP_003686.1	LY6D	lymphocyte antigen 6 complex, locus D	−3.8	−1.8	−0.5	2.7	—
NP_775876.1	KCNRG	potassium channel regulator	1.0	0.2	2.6	1.9	—
NP_005063.1	SLC12A4	solute carrier family 12 (potassium/chloride	−0.2	−0.1	1.2	1.2	—
		transporters), member 4
NP_005292.2	GPR35	G protein-coupled receptor 35	−0.8	−1.4	1.1	−0.8	—
NP_001607.1	ACVR2A	activin A receptor, type IIA	0.2	−0.5	1.4	1.0	—
NP_001687.1	ATP6V1E1	ATPase, H+ transporting, lysosomal 31 kDa, V1	−0.1	0.3	1.1	1.2	—
		subunit E1
NP_036474.1	BAMBI	BMP and activin membrane-bound inhibitor homolog	−0.8	0.7	1.3	2.4	—
		(Xenopus laevis)
NP_005177.2	CAPN1	calpain 1, (mu/l) large subunit	−1.3	−1.3	1.3	−0.2	—

[0393] Table 8-2 is a continuation of Table 8-1.

TABLE 8-2
			PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	NO
NP_001798.2	CEL	carboxyl ester lipase (bile salt-stimulated lipase)	0.0	0.0	7.4	5.4	—
NP_056034.2	EXOC7	exocyst complex component 7	−0.2	−0.2	1.9	1.2	—
NP_000034.1	FAS	Fas (TNF receptor superfamily, member 6)	0.8	0.4	2.8	2.3	8
NP_005802.1	GDF11	growth differentiation factor 11	0.7	0.9	1.3	1.9	—
NP_060456.3	GPR172B	G protein-coupled receptor 172B	0.0	0.0	5.6	4.3	—
NP_665895.1	KLK10	kallikrein-related peptidase 10	−0.9	0.4	1.2	1.6	—
NP_055414.2	MAGED2	melanoma antigen family D, 2	0.0	0.3	2.1	3.0	—
NP_000519.2	MAN2B1	mannosidase, alpha, class 2B, member 1	−0.2	−0.2	2.3	1.5	—
NP_002434.1	MSMB	microseminoprotein, beta-	0.0	0.0	5.6	4.7	—
NP_009014.2	NUDT6	nudix (nucleoside diphosphate linked moiety X)-type	0.0	0.0	1.3	0.8	—
		motif 6
NP_036528.1	PHLDA3	pleckstrin homology-like domain, family A, member 3	−0.3	−0.2	2.9	1.0	—
NP_002629.1	PI3	peptidase inhibitor 3, skin-derived	−7.6	−7.9	1.4	−0.6	—
NP_597998.1	SAT2	spermidine/spermine N1-acetyltransferase family	0.7	0.9	1.7	1.2	—
		member 2
NP_006207.1	SERPINE2	serpin peptidase inhibitor, clade E (nexin, plasminogen	−3.2	−2.5	1.3	0.7	—
		activator inhibitor type 1), member 2
NP_036303.1	TSPAN17	tetraspanin 17	0.6	0.4	1.7	1.3	—

(The values in Tables 8-1 and 8-2 shown above represent the expression difference (log 2 ratio).)

[0394] Furthermore, genes that meet a criterion described below and have GO:0005886 [plasma membrane] from GeneOntology (GO) (Tables 9 and 10) were extracted in order to obtain genes encoding proteins that are specifically presented on cell membrane of cancer stem cells.

[0395] Markers common for both proliferating and quiescent CSCs:

genes whose expression levels are in average greater than 64 in Lgr5-negative and Lgr5-positive cells; which show a greater than four-fold change in both Lgr5-negative and Lgr5-positive cells relative to primary cells; and which show a significant difference by t-test (Table 9).

TABLE 9
			PLR59	PLR123	PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	Lgr5+	Lgr5+	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	Lgr5−	Lgr5−	NO
NP_001423.1	EREG	epiregulin	2.2	3.4	2.5	3.1	0.3	-0.3	3
NP_001986.2	ACSL1	acyl-CoA synthetase long-chain family member 1	3.0	3.9	2.9	3.7	−0.2	-0.2	—
NP_005922.2	MICB	MHC class I polypeptide-related sequence B	2.3	3.8	2.2	3.7	−0.1	-0.1	—
NP_000296.2	PON2	paraoxonase 2	2.3	4.6	2.9	4.6	0.7	0.0	—
NP_003458.1	CXCR4	chemokine (C-X-C motif) receptor 4	10.2	9.5	8.5	7.8	−1.7	-1.7	—
NP_062818.1	SLCO1B3	solute carrier organic anion transporter family,	4.4	5.1	3.0	4.1	−1.4	-1.0	—
		member 1B3
NP_000280.1	PFKM	phosphofructokinase, muscle	3.3	2.3	3.3	2.1	0.0	-0.2	—
NP_057672.1	NRN1	neuritin 1	8.8	8.5	7.8	6.5	−1.0	-2.0	—
NP_060369.3	TESC	tescalcin	3.3	5.0	2.0	3.6	−1.3	-1.3	—
NP_000139.1	FUT1	fucosyltransferase 1 (galactoside 2-alpha-L-	6.9	5.9	3.1	4.3	−3.9	-1.6	—
		fucosyltransferase, H blood group)
NP_003802.1	TNFSF9	tumor necrosis factor (ligand) superfamily, member 9	4.5	4.4	5.4	5.1	0.9	0.7	6
NP_059108.1	FZD3	frizzled homolog 3 (Drosophila)	2.8	2.6	2.6	3.2	−0.2	0.6	—
NP_005810.1	STX6	syntaxin 6	2.8	2.7	4.2	3.5	1.4	0.9	—
NP_057635.1	TM7SF3	transmembrane 7 superfamily member 3	3.0	2.5	3.9	3.0	0.9	0.5	—
NP_000013.2	ADA	adenosine deaminase	2.0	2.7	2.8	2.9	0.8	0.2	—
NP_071731.1	EDAR	ectodysplasin A receptor	6.2	6.8	5.0	3.7	−1.2	-3.1	1
NP_003264.2	TM7SF2	transmembrane 7 superfamily member 2	3.6	4.3	6.9	6.7	3.2	2.4	—
NP_116265.1	JUB	jub, ajuba homolog (Xenopus laevis)	3.7	4.3	4.7	3.8	1.0	−0.5	—
NP_689740.2	SLC16A14	solute carrier family 16, member 14	5.4	6.6	5.8	6.1	0.3	−0.4	—
		(monocarboxylic acid transporter 14)
NP_065209.2	ANK1	ankyrin 1, erythrocytic	6.6	3.0	5.3	2.7	−1.3	−0.3	—

[0396] Quiescent CSC-specific markers: genes whose expression levels are in average greater than 64 in Lgr5-negative cells and are in average less than 64 in both primary cells and Lgr5-positive cells; which show a greater than 20 fold change in Lgr5-negative cells relative to Lgr5-positive cells; and which show a significant difference by t-test (Table 10).

TABLE 10
			PLR59	PLR123	PLR59	PLR123	PLR59	PLR123	SEQ
DB accession			primary	primary	primary	primary	Lgr5+	Lgr5+	ID
NO.	Abbreviation	Molecule name	Lgr5+	Lgr5+	Lgr5−	Lgr5−	Lgr5−	Lgr5−	NO
NP_006111.2	HLA-DMA	major histocompatibility complex, class II,	0.0	0.2	8.0	8.7	8.0	8.5	—
		DM alpha
NP_862830.1	AMIGO2	adhesion molecule with Ig-like domain 2	0.0	0.0	7.0	7.5	7.0	7.5	—
NP_001159449.1	PROM2	prominin 2	3.3	2.9	10.1	10.5	6.9	7.6	5
NP_076404.3	GPR87	G protein-coupled receptor 87	0.0	0.0	7.0	7.4	7.0	7.4	—
NP_722582.2	GPR110	G protein-coupled receptor 110	0.0	0.0	6.0	7.0	6.0	7.0	—
NP_112178.2	PVRL4	poliovirus receptor-related 4	−0.6	−2.7	5.8	1.7	6.4	4.4	7
NP_938205.1	FLRT3	fibronectin leucine rich transmembrane protein 3	1.3	0.2	6.8	5.4	5.5	5.2	—

[0397] After suspending in FACS buffer, cells of NOG-established cancer lines collected from mice were incubated at 4° C. for 30 minutes with rat mAb to mouse MHC I (Abcam; ab15680) and mAb to human EREG (EP27; WO2008/047723). Then, following washing once with FACS buffer, the cells were incubated at 4° C. for 30 minutes with 7-AAD Viability Dye (Beckman Coulter; A07704) as dead cell staining and secondary antibodies: PE-labeled goat F(ab′)2 fragment to mouse IgG (H+L) (Beckman Coulter; IM0855) and APC-labeled goat Ab to rat IgG (BioLegend; 405406). After washing once with FACS buffer, the cells were subjected to flow cytometry analysis. Flow cytometry analysis was performed using EPICS ALTRA. Cells negative for 7-AAD Viability Dye and mouse MHC were analyzed for EREG expression.

[0398] Lgr5-positive adherent cells and Lgr5-negative adherent cells resulting from induction by irinotecan treatment were harvested using Accutase. The cells were suspended in FACS buffer, and then incubated at 4° C. for 30 minutes with mouse mAb to human EREG. After the cells were washed once with FACS buffer, 7-AAD Viability Dye as dead cell staining and a PE-labeled goat F(ab′)2 fragment to mouse IgG (H+L) as a secondary antibody were added thereto. The cells were incubated at 4° C. for 30 minutes. Then, the cells were washed once with FACS buffer, and subjected to flow cytometry analysis. Flow cytometry analysis was performed using EPICS ALTRA. Cells negative for 7-AAD Viability Dye were analyzed for EREG expression. The result showed that the corresponding protein was expressed at a high level on cell membrane surface.

[0399] The result obtained by EREG flow cytometry analysis of primary cells from PLR59 and PLR123, and Lgr5⁺ and Lgr5⁻ cancer stem cells is shown in FIG. 37. The cells were stained using an antibody against EREG and analyzed by flow cytometry. It was demonstrated that primary cells were negative for EREG while Lgr5⁺ and Lgr5⁻ cancer stem cells were homogeneous EREG-positive cell populations. Gray indicates fluorescence intensity after cell staining with an indicated antibody; and white indicates fluorescence intensity after cell staining with a control isotype antibody.

[0400] A mononuclear cell fraction collected from human peripheral blood was used as human effector cells. Fifty ml of peripheral blood was collected from a healthy volunteer (adult male) of the inventors' company using a syringe loaded in advance with 200 μl of 1000 units/ml heparin solution (Novo-Heparin 5,000 units/5 ml for Injection; Novo Nordisk). The peripheral blood was diluted twofold with PBS(−), and then introduced into a Leucosep lymphocyte separation tube (Greiner bio-one) in advance loaded with Ficoll-Paque PLUS and subjected to centrifugation. After centrifugation (2150 rpm, room temperature, 10 minutes), the monocyte fraction layer was collected from the tube. The cells were washed once with 10% FBS/D-MEM, and then suspended at a cell density of 5×10⁶/ml in 10% FBS/D-MEM. The suspension was used as an effector cell suspension.

[0401] Target cell suspensions were prepared at the time of use. One×10⁶ cells of cancer lines were centrifuged (1200 rpm, room temperature, 5 minutes). The cell pellets were suspended in 200 μl of 0.2 mg/ml calcein-AM (Nacalai Tesque)/DMEM (10% FBS) medium. Cell suspensions in calcein-AM solution were incubated for two hours in a CO₂ incubator set to 37° C. and to a CO₂ concentration of 5%. After washing once with 10% FBS/D-MEM, the cells were adjusted to a cell density of 2×10⁵/ml with 10% FBS/D-MEM to prepare target cell suspensions.

[0402] Anti-EREG antibody was prepared at a concentration of 0.5 mg/ml, which was further diluted with 10% FBS/D-MEM to give antibody solutions. The final concentration was adjusted to 0.4, 4, and 40 μg/ml. The antibody solutions of respective concentrations or 10% FBS/D-MEM were each added at 50 μl/well to a 96-well round-bottomed plate. Then, the target cell suspensions were added at 50 μl/well to every well. The plate was incubated at room temperature for 15 minutes. Next, 100 μl of the effector cell suspension was added to each well containing target cell suspension, and antibody solution or 10% FBS/D-MEM. One-hundred μl of 10% FBS/D-MEM or 2% NP-40 solution (NP-40 substitute; Wako Pure Chemical Industries) was added to each of other wells containing 10% FBS/D-MEM and target cell suspension. The plate was centrifuged (1200 rpm, room temperature, 5 minutes) and incubated for 4 hours in a CO₂ incubator set to 37° C. and to a CO₂ concentration of 5%. The plate was centrifuged (1200 rpm, room temperature, 5 minutes), and a 100-μl aliquot of supernatant was collected from each well. The fluorescence intensity (λ_ex=490 nm, λ_em=515 nm) was determined using a spectrofluorometer. The specific calcein release rate (cytotoxicity (%)) was determined according to the following formula.

cytotoxicity (%)=(A−C)×100/(B−C)  Formula 1:

where A represents the fluorescence intensity in each well; B represents the mean value of fluorescence intensity in a well where 50 μl of target cell suspension and 100 μl of NP-40 solution were added to 50 μl of 10% FBS/D-MEM; and C represents the mean value of fluorescence intensity in a well where 50 μl of target cell suspension and 100 μl of 10% FBS/D-MEM were added to 50 μl of 10% FBS/D-MEM. This assay was carried out in triplicate, and the cytotoxicity (%) at each antibody concentration was determined using Microsoft Office Excel 2007.

[0403] The anti-EREG antibody-mediated ADCC activities against Lgr5-positive and -negative cells derived from PLR59 cells, and those against Lgr5-positive and -negative cells derived from PLR123 cells are shown in FIG. 38. The result showed that the anti-EREG antibody exerted cytotoxic activity against both Lgr5-positive and -negative cancer stem cells from PRL59 or PLR123 in a dose dependent manner whereas the control antibody had no cytotoxic activity.

[0404] To assess in vivo EREG expression, the Lgr5-positive cells were administered into the peritoneal cavities of NOG mice. In the early stage of tumor generation, EREG was expressed at a high level. In the late stage where the tumor formed specific ductal structures, EREG expression was somewhat localized to the budding clusters rather than ductal structures. EREG-positive cells were detected even after irinotecan administration to tumor-bearing mice (FIG. 54). The anti-EREG antibody was assessed for anti-tumor activity after irinotecan treatment. Effector cells are essential for the anti-EREG antibody to mediate ADCC activity. Thus, SCID mice were used as a model to assess the pharmacological efficacy of the anti-EREG antibody. Tumor growth was suppressed when the antibody was administered at the time points of days 4 and 11 after the final irinotecan administration (FIG. 57).

[0405] As a first step to assess the pharmacological efficacy based on the metastasis model, it was tested whether EREG is expressed in the metastasis model. When Lgr5-positive cells were intravenously injected into NOG mice, tumors were formed in multiple tissues including lung. Cells of the tumors formed in lungs are mostly positive for EREG (FIG. 58A). The pharmacological efficacy of the anti-EREG antibody was assessed using SCID-Beige mice where macrophages and mononuclear cells can serve as effector cells to mediate ADCC. The anti-EREG antibody was administered to mice once a week for a total of five times starting at three days after the injection of Lgr5-positive cells. The number of tumor cells in distal locations was demonstrated to be markedly reduced as compared to that in control mice (FIG. 58B). In addition, the size of each tumor was also shown to be remarkably reduced in mice administered with the antibody (FIGS. 58C and 58D).

[0406] Proliferating and quiescent CSCs were identified by immunohistochemistry using anti-Lgr5 antibody (2U2E-2), anti-HLA-DMA antibody, and anti-EREG antibody (FIG. 59 and Table 11). Proliferative CSC represents Lgr5-positive cell, while quiescent CSC represents HLA-DMA-positive and EREG-positive cell (Table 11). Lgr5-positive cells which are positive for both HLA-DMA and EREG, and Lgr5-negative cells which are positive for both HLA-DMA and EREG were present in a very small number in primary and metastatic colorectal cancer specimens isolated from colorectal cancer patients (FIG. 59). Both Lgr5-positive and -negative cells were detected in eight of 12 specimens of human colorectal cancer tissues. Meanwhile, either Lgr5-positive or Lgr5-negative cells were observed in the remaining four specimens. Throughout all specimens, Lgr5-positive cells accounted for 0.003 to 1.864%, and Lgr5-negative cells accounted for 0.001 to 10.243% (Table 11).

TABLE 11
	Property of	Case number
	CSC	1	2	3	4	5	6	7	8	9	10	11	12
Duct	Proliferative	P†	P	P	P	P	P	P	P	P	N	P	N
	Quiescent	N	P	N	P	P	P	P	P	P	P	N	P
Budding	Proliferative	P	N	N	P	P	N	N	P	P	P	N	N
area	Quiescent	N	P	N	P	N	P	N	P	N	N	N	P
Frequency	Proliferative	1.864	0.786	0.136	0.121	0.119	0.095	0.063	0.054	0.018	0.010	0.003	0.000
	Quiescent	0.000	0.243	0.000	0.187	0.001	0.228	0.045	0.065	0.003	0.003	0.000	0.073
(P† indicates that proliferating or quiescent CSCs were detected; N indicates that proliferating or quiescent CSCs were undetectable)
(Frequency indicates cell percentage)

[0407] Both Lgr5-positive and -negative CSCs were detected in the ductal and budding areas (FIG. 59). Furthermore, in ducts, Lgr5-positive and -negative CSCs were not limited to particular areas but distributed at random over the entire ducts.

[0408] The present inventors tested whether an anti-tumor effect can be expected with target therapy using a membrane protein expressed at a high level as the target in irinotecan-treated or non-treated PLR59 and PLR123. Commercially available antibodies shown in Table 12 were assessed by flow cytometry (FCM) for the binding activity to antigens expressed on the cell surface of irinotecan-treated or non-treated PLR59 or PLR123. The result is summarized in Table 13.

	TABLE 12
	Antigen name	Subtype	Manufacturer
	CD70	mIgG3	BD Pharmingen
	EDAR	mIgG1	MBL
	FAS	mIgG1	BD Pharmingen
	PROM2	mIgG2b	R & D Systems
	PVRL4	mIgG2b	R & D Systems
	TNFSF9	mIgG1	BioLegend
	PROCR	ratIgG1	BD Pharmingen
	EPCAM	mIgG2a	ABGENT

TABLE 13
	Irinotecan non-treated	Irinotecan-treated
CSC	PLR59	PLR123	PLR59	PLR123
Antibody +/−	−	+	−	+	−	+	−	+
CD70	4	1177	4	1843	NT	NT	NT	NT
EDAR	7	37	7	61	NT	NT	NT	NT
FAS	4	519	4	230	22	1871	25	1480
PROM2	NT	NT	NT	NT	29	391	19	298
PVRL4	4	8	4	19	22	109	25	129
TNFSF9	4	86	4	33	22	183	25	104
PROCR	6	19	5	13	22	519	25	127
EPCAM	7	7563	7	7701	NT	NT	NT	NT
(NT indicates not tested.)

[0409] Using Mab-ZAP and Rat-ZAP, various antibodies that had been demonstrated to have binding activity were assessed for the activity of internalization (into cells). Mab-ZAP and Rat-ZAP are anti-mouse IgG antibody and anti-rat IgG antibody, respectively, conjugated with saporin, a toxin that inhibits protein synthesis (Advanced Targeting Systems). To assess the activity of internalization into irinotecan-non-treated cells, PLR59 and PLR123 cells were seeded at a cell density of 30000 cells/80 μl/well to respective wells of a 96-well plate. Following day, each antibody solution was added at a final concentration of 0.01, 0.1, or 1 μg/ml to the respective wells. Then, Mab-ZAP or Rat-ZAP was added at a final concentration 1 μg/ml to the respective wells, and the plate was incubated at 37° C. for 72 hours in a CO₂ incubator. To assess the activity of internalization into irinotecan-treated cells, PLR59 and PLR123 cells were seeded in a 96-well plate, and irinotecan was added at a final concentration of 15 μM to each well. The plate was incubated at 37° C. for 72 hours in a CO₂ incubator. Various antibodies were assessed for the activity of internalization to cells cultured as described above in the presence or absence of irinotecan. For assay, the various antibodies were each assessed for the internalization activity into cells contained in each well where the medium was replaced with the same irinotecan-free medium as used for irinotecan-non-treated cells. Seventy-two hours after addition of antibodies and Mab-ZAP or Rat-ZAP, 10 μl of 3% SDS (Nacalai Tesque) was added to each well of the plate. The cells in the plate were lysed thoroughly by stirring the plate using a plate mixer. Then, 100 μl of CellTiter-Glo™ Luminescent Cell Viability Assay (Promega) was added to each well. The mixture in each well was assayed to determine its luminescent signal. The determined anti-tumor activity is shown in Table 14 and FIGS. 60 to 72. In FIGS. 60 to 72, the percent suppression of cell proliferation indicated by the vertical axis represents a relative value for the difference in the luminescence signal value between the mixtures in wells, one of which contained a test antibody alone (without Mab-ZAP and Rat-ZAP) and the other contained a test antibody, and Mab-ZAP or Rat-ZAP, when taking as 100% the difference in the luminescence signal value between the mixtures in wells, one of which contained a test antibody alone (without Mab-ZAP and Rat-ZAP) and the other did not contain any cells. In Table 14, symbols, −, +, ++, and +++, represent relative values for the internalization activity when a test antibody was assayed at a concentration of 1 μg/μl. The relative value refers to a relative value for the difference in the luminescence signal value between the mixtures in wells, one of which contained a test antibody alone (without Mab-ZAP and Rat-ZAP) and the other contained a test antibody, and Mab-ZAP or Rat-ZAP, when taking as 100% the difference in the luminescence signal value between the mixtures in wells, one of which contained a test antibody alone (without Mab-ZAP and Rat-ZAP) and the other did not contain any cells. Symbols, −, +, ++, and +++, indicate that the relative value is less than 5%, 5% or more and less than 15%, 15% or more and less than 25%, and 25% or more, respectively.

	TABLE 14
	PLR59	PLR123
Antigen	Irinotecan	Irinotecan-	Irinotecan	Irinotecan-
name	non-treated	treated	non-treated	treated
CD70	+++	NT	+++	NT
EDAR	++	NT	−	NT
FAS	+++	+++	+++	+++
PROM2	NT	+	NT	+
PVRL4	+	+++	−	+
TNFSF9	−	+++	−	+++
PROCR	+	++	−	+
EPCAM	+++	+++	+++	+++

[0410] As shown in FIG. 66, regarding irinotecan-non-treated PLR59 and PLR123, anti-CD70 antibody and anti-FAS antibody showed the internalization activity level of 25% or more under a condition where the anti-EPCAM antibody used as a positive control had been demonstrated to exhibit a sufficient anti-tumor activity (FIGS. 60 and 62). Furthermore, regarding PLR59, anti-EDAR antibody showed the internalization activity level of 15 to 25%, and anti-PVRL4 antibody and anti-PROCR antibody exhibited the internalization activity level of 5 to 15% (FIGS. 61, 63, and 65). Meanwhile, regarding irinotecan-treated PLR59 and PLR123, anti-FAS antibody and anti-TNFRSF9 antibody showed the internalization activity level of 25% or more, and anti-PROM2 antibody exhibited the internalization activity level of 5 to 15% (FIGS. 67, 70, and 68). Both anti-PVRL4 antibody and anti-PROCR antibody showed the internalization activity for irinotecan-treated PLR59 and PLR123, and the activity for PLR59 was greater than that for PLR123 (FIGS. 69 and 71). The result described above demonstrates that all antibodies tested have an anti-tumor effect against PLR59 and PLR123.

[0411] BMP4 was assessed for the differentiation-enhancing effect on irinotecan-non-treated and irinotecan-treated PLR59 and PLR123. To assess the differentiation-enhancing effect on irinotecan-non-treated cells, PLR59 and PLR123 cells suspended in the media where BMP4 (R&D Systems; a final concentration of 20 nM) or a control buffer was added to culture media were seeded at a cell density of 5×10⁵ cells/1.5 ml/well to each well of a 12-well plate. The cells were passaged while changing the culture media with the same type of medium 2, 4, and 7 days after seeding. To assess the differentiation-enhancing effect on irinotecan-treated cells, PLR59 or PLR123 was seeded at a cell density of 17×10⁵ cells/5 ml/flask to a 12.5-ml culture flask. Following day, irinotecan was added at a final concentration of 15 μM. The flask was incubated at 37° C. for 72 hours in a CO₂ incubator. Then, the medium in the flask was changed with a medium containing BMP4 or a control buffer. The medium was further changed with the same type of medium 2, 4, and 7 days after the initial medium change. From cells isolated 4 and 9 days after the initial medium change, RNAs were extracted using RNeasy Plus Mini Kit and RNase-Free DNase Set (QIAGEN). cDNAs were synthesized with ThermoScript RT-PCR System (Invitrogen) using the extracted RNAs as a template.

[0412] Quantitative real-time PCR was carried out using the cDNAs isolated as described above. As shown in FIG. 73, elevated CK20 levels were observed in PLR59 and PLR123 cells cultured in the presence of BMP4.

[0413] The present inventors identified cell surface molecules that are expressed specifically on cancer stem cells. The present invention provides novel anti-cancer drugs and reagents for detecting cancer stem cells, which use antibodies against the cell surface molecules.