Fc REGION VARIANT

[0001] The present invention relates to Fc region variants in which amino acid alterations have been introduced into an antibody Fc region to reduce binding activities to all activating FcγRs, in particular FcγRIIa (R type), while maintaining a binding activity to FcγRIIb, compared to a polypeptide containing a naturally-occurring human IgG Fc region; polypeptides comprising the Fc region variants; and pharmaceutical compositions comprising the polypeptides.

[0002] Antibodies are drawing attention as pharmaceuticals as they are highly stable in plasma and have few side effects. A number of IgG-type antibody pharmaceuticals are now available on the market and many antibody pharmaceuticals are currently under development (Non-patent Documents 1 and 2). Meanwhile, various technologies applicable to second-generation antibody pharmaceuticals have been reported, including those that enhance effector function, antigen-binding ability, pharmacokinetics and stability, and those that reduce the risk of immunogenicity (Non-patent Document 3). In general, the requisite dose of an antibody pharmaceutical is very high. This in turn has led to problems such as high production cost as well as difficulty in producing subcutaneous formulations. In theory, the dose of an antibody pharmaceutical may be reduced by improving antibody pharmacokinetics or improving the affinity between antibodies and antigens.

[0003] Literature has reported methods for improving antibody pharmacokinetics using artificial substitution of amino acids in constant regions (Non-patent Documents 4 and 5). Similarly, affinity maturation has been reported as a technology for enhancing antigen-binding ability or antigen-neutralizing activity (Non-patent Document 6). This technology enables enhancement of antigen-binding activity by introduction of amino acid mutations into the CDR of a variable region or such. The enhancement of antigen-binding ability enables improvement of in vitro biological activity or reduction of dosage, and further enables improvement of in vivo efficacy (Non-patent Document 7).

[0004] The antigen-neutralizing capacity of a single antibody molecule depends on its affinity. By increasing the affinity, an antigen can be neutralized by a smaller amount of an antibody. Various methods can be used to enhance antibody affinity (Non-patent Document 6). Furthermore, if affinity could be made infinite by covalently binding an antibody to an antigen, a single antibody molecule could neutralize one antigen molecule (a divalent antibody can neutralize two antigen molecules). However, in the methods to date, for a single antibody molecule, binding to one antigen molecule (two antigens if bivalent) is the limit. On the other hand, it has been recently reported that the use of antigen-binding molecules that bind to antigens in a pH-dependent manner allows a single antigen-binding molecule to bind to multiple antigen molecules (Patent Document 1 and Non-patent Document 8). Under the neutral condition in plasma, pH-dependent antigen-binding molecules bind strongly to antigens, and release the antigens under the acidic conditions in endosomes. Furthermore, the antigen-binding molecules can bind to antigen again when they are recycled into the plasma by FcRn after releasing the antigens; therefore, a single pH-dependent antigen-binding molecule can repeatedly bind to multiple antigens.

[0005] Furthermore, it has been reported that since pH-dependent antigen-binding molecules altered to enhance FcRn binding under a neutral condition (pH 7.4) have an effect of being able to bind repeatedly to antigens and an effect of eliminating antigens from plasma, administration of such antigen-binding molecules enables antigen elimination from plasma (Patent Document 2). A pH-dependent antigen-binding molecule comprising an ordinary IgG antibody Fc region showed almost no binding to FcRn under neutral conditions. Therefore, uptake of complexes formed between the antigen-binding molecule and antigen into cells may be mainly through non-specific uptake. According to this report, pH-dependent antigen-binding molecules that have been altered to enhance FcRn binding under a neutral condition (pH 7.4) can further accelerate the antigen elimination than the pH-dependent antigen-binding molecule comprising an ordinary IgG antibody Fc region (Patent Document 2).

[0006] Since plasma retention of antigens is very short compared to antibodies having an FcRn-mediated recycling mechanism, binding of an antigen in plasma to the antibodies having a recycling mechanism (wherein the binding is not pH-dependent) usually prolongs its retention period in plasma and increases the plasma antigen concentration. For example, in the case of plasma antigens with multiple types of physiological functions, even if one type of physiological activity is blocked by antibody binding, symptoms caused by other physiological functions may be exacerbated by the plasma concentration of the antigens due to antibody binding. Since there are cases where eliminating plasma antigens is favorable from such viewpoint, methods similar to those described above for applying alterations to the Fc region to enhance FcRn-binding with the objective of accelerating antigen elimination have been reported, but so far there has been no report on other methods for accelerating antigen elimination.

[0007] In addition, side effects of several therapeutic antibodies originating from interaction between IgG and FcγR have been reported. For example, it is known that a group of patients who were administered with bevacizumab, an anti-VEGF antibody, have an increased rate of developing thromboembolism (Non-patent Document 9). Furthermore, thromboembolism has been similarly observed in clinical development tests of an antibody against the CD40 ligand, and the clinical trial was discontinued (Non-patent Document 10). FcγRIIa, an activating Fcγ receptor, is expressed on platelet cells (Non-patent Document 11), and later studies using animal models and such have suggested that both of the administered antibodies cause platelets to aggregate by binding to FcγRIIa on the platelets, and form blood clots as a result (Non-patent Documents 12 and 13). It has been reported that in patients with systemic lupus erythematosus, which is an autoimmune disease, platelets are activated via an FcγRIIa-dependent mechanism and that platelet activation correlates with the severity of symptoms (Non-patent Document 14).

[0008] Furthermore, it has been reported from studies using animal models that multivalent antigen-antibody immune complexes induce anaphylaxis via activating FcγRs (Non-patent Document 15).

[0009] In addition, there are reports that incorporation of multivalent antigen-antibody immune complexes via activating FcγRs causes an increase in antibody titer production against the antigen (Non-patent Documents 16 and 17). This result suggests the possibility that antibodies against a therapeutic antibody itself may be more easily produced when it is a therapeutic antibody that recognizes multivalent antigens. If antibodies against a therapeutic antibody are produced, blood kinetics of the therapeutic antibody becomes worse or neutralizing antibodies may weaken the effects of the therapeutics.

[0010] This way, an antibody binds to a multivalent antigen to form an immune complex, and interaction of the complex with activating FcγRs may induce various side effects, and this will reduce the value of the antibody as pharmaceuticals.

• [0011] [Patent Document 1] WO2009/125825
• [0012] [Patent Document 2] WO2011/122011

• [0013] [Non-patent Document 1] Monoclonal antibody successes in the clinic, Janice M Reichert, Clark J Rosensweig, Laura B Faden & Matthew C Dewitz, Nat. Biotechnol. (2005) 23, 1073-1078
• [0014] [Non-patent Document 2] Pavlou A K, Belsey M J., The therapeutic antibodies market to 2008, Eur. J. Pharm. Biopharm. (2005) 59 (3), 389-396
• [0015] [Non-patent Document 3] Kim S J, Park Y, Hong H J., Antibody engineering for the development of therapeutic antibodies, Mol. Cells. (2005) 20 (1), 17-29
• [0016] [Non-patent Document 4] Hinton P R, Xiong J M, Johlfs M G, Tang M T, Keller S, Tsurushita N, J. Immunol. (2006) 176 (1), 346-356
• [0017] [Non-patent Document 5] Ghetie V, Popov S, Borvak J, Radu C, Matesoi D, Medesan C, Ober R J, Ward E S., Nat. Biotechnol. (1997) 15 (7), 637-640
• [0018] [Non-patent Document 6] Rajpal A, Beyaz N, Haber L, Cappuccilli G, Yee H, Bhatt R R, Takeuchi T, Lerner R A, Crea R., Proc. Natl. Acad. Sci. USA. (2005) 102 (24), 8466-8471
• [0019] [Non-patent Document 7] Wu H, Pfarr D S, Johnson S, Brewah Y A, Woods R M, Patel N K, White W I, Young J F, Kiener P A., J. Mol. Biol. (2007) 368, 652-665
• [0020] [Non-patent Document 8] Igawa T, et al., Nat. Biotechnol. (2010) 28, 1203-1207
• [0021] [Non-patent Document 9] Scappaticci F A, Skillings J R, Holden S N, Gerber H P, Miller K, Kabbinavar F, Bergsland E, Ngai J, Holmgren E, Wang J, Hurwitz H., Arterial thromboembolic events in patients with metastatic carcinoma treated with chemotherapy and bevacizumab., J. Natl. Cancer Inst. (2007) 99 (16), 1232-1239
• [0022] [Non-patent Document 10] Boumpas D T, Furie R, Manzi S, Illei G G, Wallace D J, Balow J E, Vaishnaw A, A short course of BG9588 (anti-CD40 ligand antibody) improves serologic activity and decreases hematuria in patients with proliferative lupus glomerulonephritis., Arthritis. Rheum. (2003) 48 (3), 719-727.
• [0023] [Non-patent Document 11] Mackay M, Stanevsky A, Wang T, Aranow C, Li M, Koenig S, Ravetch J V, Diamond B., Selective dysregulation of the FcgammaIIB receptor on memory B cells in SLE., J. Exp. Med. (2006) 203 (9), 2157-2164
• [0024] [Non-patent Document 12] Meyer T, Robles-Carrillo L, Robson T, Langer F, Desai H, Davila M, Amaya M, Francis J L, Amirkhosravi A., Bevacizumab immune complexes activate platelets and induce thrombosis in FCGR2A transgenic mice., J. Thromb. Haemost. (2009) 7 (1), 171-181
• [0025] [Non-patent Document 13] Robles-Carrillo L, Meyer T, Hatfield M, Desai H, Davila M, Langer F, Amaya M, Garber E, Francis J L, Hsu Y M, Amirkhosravi A., Anti-CD40L immune complexes potently activate platelets in vitro and cause thrombosis in FCGR2A transgenic mice., J. Immunol. (2010) 185 (3), 1577-1583
• [0026] [Non-patent Document 14] Duffau P, Seneschal J, Nicco C, Richez C, Lazaro E, Douchet I, Bordes C, Viallard J F, Goulvestre C, Pellegrin J L, Weil B, Moreau J F, Batteux F, Blanco P., Platelet CD154 potentiates interferon-alpha secretion by plasmacytoid dendritic cells in systemic lupus erythematosus., Sci. Transl. Med. (2010) 2 (47), 47-63
• [0027] [Non-patent Document 15] Bruhns P., Properties of mouse and human IgG receptors and their contribution to disease models. Blood. (2012) 119, 5640-9.
• [0028] [Non-patent Document 16] Hjelm F, Carlsson F, Getahun A, Heyman B., Antibody-mediated regulation of the immune response. Scand J Immunol. (2006) 64(3), 177-84. [Non-patent Document 17] Wernersson S, Karlsson M C, Dahlstrom J, Mattsson R, Verbeek J S, Heyman B., IgG-mediated enhancement of antibody responses is low in Fc receptor gamma chain-deficient mice and increased in Fc gamma RII-deficient mice. J Immunol. (1999) 163, 618-22.

[0029] The present invention was achieved in view of the above circumstances. An objective of the present invention is to provide molecules that overcome the defect which originates from binding to activating FcγRs, by introducing an amino acid alteration into an antibody Fc region to accelerate antigen elimination. More specifically, an objective of the present invention is to provide Fc region variants whose binding activities to all activating FcγRs, in particular FcγRIIa (R type), can be reduced while their FcγRIIb-binding activity is maintained, as compared to those of a polypeptide containing a naturally-occurring IgG antibody Fc region; polypeptides comprising the Fc region variants; and pharmaceutical compositions comprising the polypeptides.

[0030] The present inventors conducted dedicated research on Fc region variants whose binding activities to all activating FcγRs, in particular FcγRIIa (R type), can be decreased while their FcγRIIb-binding activity is maintained, when compared to a polypeptide containing a naturally-occurring IgG Fc region, by introducing amino acid alterations into an Fc region; and polypeptides comprising the Fc region variants. As a result, the present inventors discovered that binding activities to all activating FcγRs, in particular FcγRIIa (R type), can be decreased while maintaining FcγRIIb-binding activity by combining other amino acid alterations into an Fc region variant with alteration of the amino acid at position 238 in the Fc region according to EU numbering.

[0031] More specifically, the present invention relates to the following:

[1] an Fc region variant, wherein the variant comprises alteration of amino acid at position 238 of the Fc region according to EU numbering and any one of the amino acids of (a) to (k) below, wherein an FcγRIIb-binding activity of the variant is maintained and binding activities of the variant to all activating FcγRs are decreased when compared to those of a native IgG Fc region:

[0032] (a) amino acid at position 235 of the Fc region according to EU numbering;

[0033] (b) amino acid at position 237 of the Fc region according to EU numbering;

[0034] (c) amino acid at position 241 of the Fc region according to EU numbering;

[0035] (d) amino acid at position 268 of the Fc region according to EU numbering;

[0036] (e) amino acid at position 295 of the Fc region according to EU numbering;

[0037] (f) amino acid at position 296 of the Fc region according to EU numbering;

[0038] (g) amino acid at position 298 of the Fc region according to EU numbering;

[0039] (h) amino acid at position 323 of the Fc region according to EU numbering;

[0040] (i) amino acid at position 324 of the Fc region according to EU numbering;

[0041] (j) amino acid at position 330 of the Fc region according to EU numbering; and

[0042] (k) at least two amino acids selected from (a) to (j);

[2] the variant of [1] above, wherein at least two amino acids selected in (k) of [1] above are any one of the following amino acid combinations (1) to (3):

[0043] (1) amino acids at positions 241, 268, 296, and 324 of the Fc region according to EU numbering;

[0044] (2) amino acids at positions 237, 241, 296, and 330 of the Fc region according to EU numbering; and

[0045] (3) amino acids at positions 235, 237, 241, and 296 of the Fc region according to EU numbering;

[3] an Fc region variant, wherein the amino acid at position 238 of the Fc region according to EU numbering is Asp, and wherein the variant comprises any one of the amino acids of (a) to (k) below, wherein an FcγRIIb-binding activity of the variant is maintained and binding activities of the variant to all activating FcγRs are decreased when compared to those of a native IgG Fc region:

[0046] (a) Phe at amino acid position 235 of the Fc region according to EU numbering;

[0047] (b) Gln or Asp at amino acid position 237 of the Fc region according to EU numbering;

[0048] (c) Met or Leu at amino acid position 241 of the Fc region according to EU numbering;

[0049] (d) Pro at amino acid position 268 of the Fc region according to EU numbering;

[0050] (e) Met or Val at amino acid position 295 of the Fc region according to EU numbering;

[0051] (f) Glu, His, Asn, or Asp at amino acid position 296 of the Fc region according to EU numbering;

[0052] (g) Ala or Met at amino acid position 298 of the Fc region according to EU numbering;

[0053] (h) Ile at amino acid position 323 of the Fc region according to EU numbering;

[0054] (i) Asn or His at amino acid position 324 of the Fc region according to EU numbering;

[0055] (j) His or Tyr at amino acid position 330 of the Fc region according to EU numbering; and

[0056] (k) at least two amino acids selected from (a) to (j);

[4] an Fc region variant, wherein the amino acid at position 238 of the Fc region according to EU numbering is Asp, and wherein the variant comprises the amino acids of any one of (1) to (3) below:

[0057] (1) Met at amino acid position 241, Pro at amino acid position 268, Glu at amino acid position 296, and His at amino acid position 324 of the Fc region according to EU numbering;

[0058] (2) Gln or Asp at amino acid position 237, Met at amino acid position 241, Glu at amino acid position 296, and His at amino acid position 330 of the Fc region according to EU numbering; and

[0059] (3) Phe at amino acid position 235, Gln or Asp at amino acid position 237, Met at amino acid position 241, and Glu at amino acid position 296 of the Fc region according to EU numbering;

[5] an Fc region variant, wherein the variant comprises alterations of amino acids at positions 238 and 271 of the Fc region according to EU numbering and any one of the amino acids of (a) to (h) below, wherein an FcγRIIb-binding activity of the variant is maintained and binding activities of the variant to all activating FcγRs are decreased when compared to those of a native IgG Fc region:

[0060] (a) amino acid at position 234 of the Fc region according to EU numbering;

[0061] (b) amino acid at position 235 of the Fc region according to EU numbering;

[0062] (c) amino acid at position 236 of the Fc region according to EU numbering;

[0063] (d) amino acid at position 237 of the Fc region according to EU numbering;

[0064] (e) amino acid at position 239 of the Fc region according to EU numbering;

[0065] (f) amino acid at position 265 of the Fc region according to EU numbering;

[0066] (g) amino acid at position 267 of the Fc region according to EU numbering; and

[0067] (h) amino acid at position 297 of the Fc region according to EU numbering;

[6] the variant of [5] above, wherein the amino acid alteration is a combination of amino acid alterations of any one of (1) to (3) below:

[0068] (1) amino acids at positions 233, 238, 264, 267, 268, and 271 of the Fc region according to EU numbering;

[0069] (2) amino acids at positions 233, 237, 238, 264, 267, 268, 271, 296, 297, 330, and 396 of the Fc region according to EU numbering; and

[0070] (3) amino acids at positions 233, 238, 264, 267, 268, 271 and 296 of the Fc region according to EU numbering;

[7] an Fc region variant, wherein the amino acid at position 238 is Asp and the amino acid at position 271 is Gly in the Fc region according to EU numbering, and wherein the variant comprises any one of the amino acids of (a) to (h) below, wherein an FcγRIIb-binding activity of the variant is maintained and binding activities of the variant to all activating FcγRs are decreased when compared to those of a native IgG Fc region:

[0071] (a) Ala, His, Asn, Lys, or Arg at amino acid position 234 of the Fc region according to EU numbering;

[0072] (b) Ala at amino acid position 235 of the Fc region according to EU numbering;

[0073] (c) Gln at amino acid position 236 of the Fc region according to EU numbering;

[0074] (d) Arg or Lys at amino acid position 237 of the Fc region according to EU numbering;

[0075] (e) Lys at amino acid position 239 of the Fc region according to EU numbering;

[0076] (f) Lys, Asn, Arg, Ser, or Val at amino acid position 265 of the Fc region according to EU numbering;

[0077] (g) Lys, Arg, or Tyr at amino acid position 267 of the Fc region according to EU numbering; and

[0078] (h) Ala at amino acid position 297 of the Fc region according to EU numbering;

[8] an Fc region variant, wherein the amino acid at position 238 is Asp and the amino acid at position 271 is Gly in the Fc region according to EU numbering, and wherein the variant comprises the amino acids of any one of (1) to (3) below:

[0079] (1) Asp at amino acid position 233, Asp at amino acid position 238, Ile at amino acid position 264, Arg at amino acid position 267, Glu at amino acid position 268, and Gly at amino acid position 271 of the Fc region according to EU numbering;

[0080] (2) Asp at amino acid position 233, Asp at amino acid position 237, Asp at amino acid position 238, Ile at amino acid position 264, Ala at amino acid position 267, Glu at amino acid position 268, Gly at amino acid position 271, Asp at amino acid position 296, Ala at amino acid position 297, Arg at amino acid position 330, and Met at amino acid position 396 of the Fc region according to EU numbering; and

[0081] (3) Asp at amino acid position 233, Asp at amino acid position 238, Ile at amino acid position 264, Arg at amino acid position 267, Pro at amino acid position 268, Gly at amino acid position 271, and Glu at amino acid position 296 of the Fc region according to EU numbering;

[9] the Fc region variant of any one of [1] to [8] above, wherein its complement-binding is also decreased;
[10] the Fc region variant of [9] above, wherein the Fc region variant with decreased complement-binding comprises an amino acid alteration at position 322 of the Fc region according to EU numbering, or amino acid alterations at positions 327, 330, and 331 of the Fc region according to EU numbering;
[11] the Fc region variant of [9] above, wherein the amino acid at position 322 is Ala or Glu in the Fc region according to EU numbering; or the amino acid at position 327 is Gly, the amino acid at position 330 is Ser, and the amino acid at position 331 is Ser in the Fc region according to EU numbering;
[12] an Fc region variant, wherein the variant comprises alterations of amino acids at positions 238, 271, 327, 330, and 331 of the Fc region according to EU numbering, wherein an FcγRIIb-binding activity of the variant is maintained and binding activities of the variant to all activating FcγRs are decreased when compared to those of a native IgG Fc region;
[13] the variant of [12] above, wherein the variant further comprises an amino acid alteration of any one of (a) to (e) below:

[0082] (a) the amino acid at position 233 of the Fc region according to EU numbering;

[0083] (b) the amino acid at position 237 of the Fc region according to EU numbering;

[0084] (c) the amino acid at position 264 of the Fc region according to EU numbering;

[0085] (d) the amino acid at position 267 of the Fc region according to EU numbering; and

[0086] (e) the amino acid at position 268 of the Fc region according to EU numbering;

[14] the variant of [13] above, wherein the amino acid alteration is a combination of amino acid alterations of any one of (1) to (4) below:

[0087] (1) amino acids at positions 237, 238, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering;

[0088] (2) amino acids at positions 233, 237, 238, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering;

[0089] (3) amino acids at positions 238, 267, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering; and

[0090] (4) amino acids at positions 238, 264, 267, 271, 327, 330, and 331 of the Fc region according to EU numbering;

[15] an Fc region variant, wherein the amino acid at position 238 is Asp, the amino acid at position 271 is Gly, the amino acid at position 327 is Gly, the amino acid at position 330 is Ser, and the amino acid at position 331 is Ser in the Fc region according to EU numbering, wherein an FcγRIIb-binding activity of the variant is maintained and binding activities of the variant to all activating FcγRs are decreased when compared to those of a native IgG Fc region;
[16] the variant of [15] above, wherein the variant further comprises any one of the amino acids of (a) to (h) below:

[0091] (a) Asp at amino acid position 233 of the Fc region according to EU numbering;

[0092] (b) Asp at amino acid position 237 of the Fc region according to EU numbering;

[0093] (c) Ile at amino acid position 264 of the Fc region according to EU numbering;

[0094] (d) Ala at amino acid position 267 of the Fc region according to EU numbering; and

[0095] (e) Asp or Glu at amino acid position 268 of the Fc region according to EU numbering;

[17] an Fc region variant, wherein the amino acid at position 238 is Asp and the amino acid at position 271 is Gly in the Fc region according to EU numbering, wherein the variant also comprises the amino acids of any one of (1) to (4) below:

[0096] (1) Asp at amino acid position 237, Asp at amino acid position 238, Asp or Glu at amino acid position 268, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, and Ser at amino acid position 331 of the Fc region according to EU numbering;

[0097] (2) Asp at amino acid position 233, Asp at amino acid position 237, Asp at amino acid position 238, Asp at amino acid position 268, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, and, Ser at amino acid position 331 of the Fc region according to EU numbering;

[0098] (3) Asp at amino acid position 238, Ala at amino acid position 267, Glu at amino acid position 268, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, and Ser at amino acid position 331 of the Fc region according to EU numbering; and

[0099] (4) Asp at amino acid position 238, Ile at amino acid position 264, Ala at amino acid position 267, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, and Ser at amino acid position 331 of the Fc region according to EU numbering;

[18] the Fc region variant of any one of [1] to [17] above, wherein the variant has an FcγRIIb-binding activity that is at least 80% of the binding amount of a native IgG Fc region, and has an FcγRIIaR-binding activity that is 30% or less of the binding amount of the native IgG Fc region;
[19] the Fc region variant of any one of [1] to [18] above, wherein a ratio of the FcγRIIb-binding activity of the variant relative to the binding activity of a polypeptide comprising the native IgG Fc region is at least 0.75, and the ratios of the binding activities to all activating FcγRs are 0.2 or less;
[20] the Fc region variant of [19] above, wherein additionally a ratio of an FcγRIIaR-binding activity of the variant relative to the binding activity of a polypeptide comprising the native IgG Fc region is 0.1 or less;
[21] a polypeptide comprising the Fc region variant of any one of [1] to [20] above;
[22] the polypeptide of [21] above, wherein the polypeptide comprising the Fc region variant is an IgG antibody;
[23] the polypeptide of [21] above, wherein the polypeptide comprising the Fc region variant is an Fc fusion protein molecule;
[24] a pharmaceutical composition comprising the polypeptide of any one of [21] to [23] above, and a medically acceptable carrier;
[25] the polypeptide of [21] above, which further comprises an antigen-binding domain whose antigen-binding activity changes depending on an ion concentration condition;
[26] the polypeptide of [25] above, wherein the ion concentration condition is a calcium ion concentration condition;
[27] the polypeptide of [26] above, wherein the aforementioned antigen-binding domain has a lower antigen-binding activity under a low calcium ion concentration condition than under a high calcium ion concentration condition;
[28] the polypeptide of any one of [25] to [27] above, wherein the ion concentration condition is a pH condition;
[29] the polypeptide of [28] above, wherein the antigen-binding domain has a lower antigen-binding activity in an acidic pH range condition than under a neutral pH range condition;
[30] the polypeptide of any one of [25] to [29] above, wherein the polypeptide comprising the Fc region variant is an IgG antibody;
[31] the polypeptide of any one of [25] to [29] above, wherein the polypeptide comprising the Fc region variant is an Fc fusion protein molecule;
[32] a pharmaceutical composition comprising the polypeptide of any one of [25] to [31] above and a medically acceptable carrier;
[33] the pharmaceutical composition of [32] above for promoting elimination of an antigen from plasma, wherein the antigen binds to the antigen-binding domain of the polypeptide of any one of [25] to [31] above, and is in the plasma;
[34] use of the polypeptide of any one of [25] to [31] above for promoting elimination of an antigen from plasma, wherein the antigen binds to the antigen-binding domain of the polypeptide, and is in the plasma;
[35] a method of reducing bindings of a polypeptide comprising an Fc region to all activating FcγRs while maintaining an FcγRIIb-binding activity of the polypeptide, which comprises altering the amino acid at position 238 of the Fc region according to EU numbering and at least one amino acid selected from the amino acids at positions 235, 237, 241, 268, 295, 296, 298, 323, 324, and 330 of the Fc region according to EU numbering to other amino acids;
[36] the method of [35] above, wherein the amino acid alteration of the Fc region is a substitution of the amino acid at position 238 with Asp, a substitution of the amino acid at position 235 with Phe, a substitution of the amino acid at position 237 with Gln, a substitution of the amino acid at position 241 with Met or Leu, a substitution of the amino acid at position 268 with Pro, a substitution of the amino acid at position 295 with Met or Val, a substitution of the amino acid at position 296 with Glu, His, Asn, or Asp, a substitution of the amino acid at position 298 with Ala or Met, a substitution of the amino acid at position 323 with Ile, a substitution of the amino acid at position 324 with Asn or His, and a substitution of the amino acid at position 330 with His or Tyr according to EU numbering;
[37] a method of producing a polypeptide comprising an Fc region variant, wherein the method comprises altering the amino acid at position 238 of the Fc region according to EU numbering and at least one amino acid selected from the amino acids at positions 235, 237, 241, 268, 295, 296, 298, 323, 324, and 330 of the Fc region according to EU numbering to other amino acids, wherein bindings to all activating FcγRs are decreased while an FcγRIIb-binding activity is maintained as compared to those of the polypeptide before the altering;
[38] the method of [37] above, wherein the amino acid alteration of the Fc region is a substitution of the amino acid at position 238 with Asp, substitution of the amino acid at position 235 with Phe, substitution of the amino acid at position 237 with Gln, substitution of the amino acid at position 241 with Met or Leu, substitution of the amino acid at position 268 with Pro, substitution of the amino acid at position 295 with Met or Val, substitution of the amino acid at position 296 with Glu, His, Asn, or Asp, substitution of the amino acid at position 298 with Ala or Met, substitution of the amino acid at position 323 with Ile, substitution of the amino acid at position 324 with Asn or His, and substitution of the amino acid at position 330 with His or Tyr according to EU numbering;
[39] a method of reducing binding activities of a polypeptide comprising an Fc region to all activating FcγRs while maintaining an FcγRIIb-binding activity at a similar level in comparison to those of a native IgG, wherein the method comprises combining and introducing an amino acid alteration(s) that increases the FcγRIIb-binding activity by two-fold or more as compared to that of a native IgG Fc region, and an amino acid alteration(s) that decreases the binding activities to all FcγRs;
[40] the method of [39] above, wherein the amino acid alteration(s) that increases the FcγRIIb-binding activity by two-fold or more as compared to that of the native IgG Fc region is an amino acid alteration(s) of Table 11;
[41] the method of [39] or [40] above, wherein the amino acid alteration(s) that decreases the binding activities to all FcγRs is an alteration(s) of at least one amino acid selected from the amino acids at positions 234, 235, 236, 237, 239, 265, 267, and 297 of the Fc region according to EU numbering to another amino acid;
[42] the method of any one of [39] to [41] above, wherein the amino acid alteration(s) of the Fc region is a substitution of the amino acid at position 234 with Ala, His, Asn, Lys, or Arg, a substitution of the amino acid at position 235 with Ala, a substitution of the amino acid at position 236 with Gln, a substitution of the amino acid at position 237 with Arg or Lys, a substitution of the amino acid at position 239 with Lys, a substitution of the amino acid at position 265 with Lys, Asn, Arg, Ser, or Val, a substitution of the amino acid at position 267 with Lys, Arg, or Tyr, and a substitution of the amino acid at position 297 with Ala according to EU numbering;
[43] the method of any one of [35], [36], and [39] to [42] above, wherein the FcγRIIb-binding activity is maintained with at least 80% of the binding amount of a native IgG Fc region, and an FcγRIIaR-binding activity is reduced to 30% or less of the binding amount of the native IgG Fc region;
[44] the method of any one of [35], [36], and [39] to [43] above, wherein a ratio of the FcγRIIb-binding activity relative to the binding activity of a polypeptide comprising the native IgG Fc region is maintained at 0.75 at least, and the ratios of the binding activities to all activating FcγRs are reduced to 0.2 or less;
[45] the method of [44] above, wherein additionally a ratio of an FcγRIIaR-binding activity relative to the binding activity of a polypeptide comprising the native IgG Fc region is reduced to 0.05 or less;
[46] a method of producing a polypeptide comprising an Fc region variant, wherein binding activities to all activating FcγRs are decreased while an FcγRIIb-binding activity is maintained at a similar level in comparison to those of a native IgG, wherein the method comprises combining and introducing an amino acid alteration(s) that increases the FcγRIIb-binding activity by two-fold or more as compared to that of a native IgG Fc region, and an amino acid alteration(s) that decreases the binding activities to all FcγRs;
[47] the method of [46] above, wherein the amino acid alteration(s) that increases the FcγRIIb-binding activity by two-fold or more as compared to that of the native IgG Fc region is an amino acid alteration of Table 11;
[48] the method of [46] or [47] above, wherein the amino acid alteration(s) that decreases the binding activities to all FcγRs is an alteration of at least one amino acid selected from the amino acids at positions 234, 235, 236, 237, 239, 265, 267, and 297 of an Fc region according to EU numbering to another amino acid;
[49] the method of any one of [46] to [48] above, wherein the amino acid alteration(s) of the Fc region is a substitution of the amino acid at position 234 with Ala, His, Asn, Lys, or Arg, a substitution of the amino acid at position 235 with Ala, a substitution of the amino acid at position 236 with Gln, a substitution of the amino acid at position 237 with Arg or Lys, a substitution of the amino acid at position 239 with Lys, a substitution of the amino acid at position 265 with Lys, Asn, Arg, Ser, or Val, a substitution of the amino acid at position 267 with Lys, Arg, or Tyr, and a substitution of the amino acid at position 297 with Ala according to EU numbering;
[50] the method of any one of [37], [38], and [46] to [49] above, wherein the FcγRIIb-binding activity is maintained with at least 80% of the binding amount of a native IgG Fc region, and the binding activities to all activating FcγRs are reduced to 30% or less of the binding amount of the native IgG Fc region;
[51] the method of any one of [37], [38], and [46] to [50] above, wherein a ratio of the FcγRIIb-binding activity relative to the binding activity of a polypeptide comprising the native IgG Fc region is maintained at 0.75 at least, and the ratios of binding activities to all activating FcγRs are decreased to 0.2 or less;
[52] the method of [51] above, wherein additionally a ratio of the FcγRIIaR-binding activity relative to the binding activity of a polypeptide comprising the native IgG Fc region is reduced to 0.1 or less;
[53] the method of any one of [37], [38], and [46] to [52] above, wherein the method further comprises combining and introducing an alteration(s) that decreases complement-binding;
[54] the method of [53] above, wherein the alteration(s) that decreases complement-binding is an amino acid alteration at position 322 of the Fc region according to EU numbering, or amino acid alterations at positions 327, 330, and 331 of the Fc region according to EU numbering;
[55] the method of [53] above, wherein the alteration(s) that decreases complement-binding is a substitution of the amino acid at position 322 with Ala or Glu in the Fc region according to EU numbering, or alternatively, a substitution of the amino acid at position 327 with Gly, a substitution of the amino acid at position 330 with Ser, and a substitution of the amino acid at position 331 with Ser in the Fc region according to EU numbering;
[56] a method of reducing bindings of a polypeptide comprising an Fc region to all activating FcγRs while maintaining an FcγRIIb-binding activity of the polypeptide, which comprises altering the amino acids at positions 238, 271, 327, 330, and 331 of the Fc region according to EU numbering to other amino acids, or further comprises altering at least one amino acid selected from the amino acids at positions 233, 237, 264, 267, and 268 of the Fc region according to EU numbering to another amino acid;
[57] the method of [56] above, wherein the amino acid alteration of the Fc region is a substitution of the amino acid at position 238 with Asp, a substitution of the amino acid at position 271 with Gly, a substitution of the amino acid at position 327 with Gly, a substitution of the amino acid at position 330 with Ser, a substitution of the amino acid at position 331 with Ser, a substitution of the amino acid at position 233 with Asp, a substitution of the amino acid at position 237 with Asp, a substitution of the amino acid at position 264 with Ile, a substitution of the amino acid at position 267 with Ala, and a substitution of the amino acid at position 268 with Asp or Glu in the Fc region according to EU numbering;
[58] a method of producing a polypeptide comprising an Fc region variant, wherein the method comprises altering the amino acids at positions 238, 271, 327, 330, and 331 of the Fc region according to EU numbering to other amino acids, or further comprising altering at least one amino acid selected from the amino acids at positions 233, 237, 264, 267, and 268 of the Fc region according to EU numbering to another amino acid, wherein bindings to all activating FcγRs are decreased and complement-binding is decreased while an FcγRIIb-binding activity is maintained as compared to those of the polypeptide before the altering; and
[59] the method of [58] above, wherein the amino acid alteration of the Fc region is a substitution of the amino acid at position 238 with Asp, a substitution of the amino acid at position 271 with Gly, a substitution of the amino acid at position 327 with Gly, a substitution of the amino acid at position 330 with Ser, a substitution of the amino acid at position 331 with Ser, a substitution of the amino acid at position 233 with Asp, a substitution of the amino acid at position 237 with Asp, a substitution of the amino acid at position 264 with Ile, a substitution of the amino acid at position 267 with Ala, and a substitution of the amino acid at position 268 with Asp or Glu in the Fc region according to EU numbering.

[0100] Furthermore, the present invention relates to methods of decreasing the binding activities of an Fc region to all activating FcγRs, in particular FcγRIIa (R type), while maintaining its FcγRIIb-binding activity by introducing the Fc-region amino-acid alterations of the present invention. The present invention also relates to methods for suppressing production of antibodies against polypeptides comprising the Fc region by introducing the Fc-region amino-acid alterations of the present invention.

[0101] Furthermore, the present invention relates to methods for promoting elimination of a disease-causing antigen in plasma, which are accomplished by an Fc region variant produced by introducing the Fc-region amino-acid alterations of the present invention, and a polypeptide which has an activity to bind to the antigen present in soluble form in plasma and comprises an antigen-binding domain whose binding activity towards the antigen changes according to the ion concentration condition. The present invention also relates to the use of an Fc region variant produced by introducing the Fc-region amino acid alterations of the present invention, and a polypeptide having an activity to bind to a disease-causing antigen present in soluble form in plasma and comprising an antigen-binding domain whose binding activity towards the antigen changes according to the ion concentration condition, wherein the use is for promoting elimination of the antigen in plasma.

[0102] The present invention also relates to a therapeutic or preventive agent for immune inflammatory diseases that comprises a polypeptide of the present invention. Furthermore, the present invention relates to a method for treating or preventing immune inflammatory diseases, which comprises the step of administering a polypeptide of the present invention to a subject. In addition, the present invention relates to a kit for use in the method of the present invention for treating or preventing immune inflammatory diseases, which comprises a polypeptide of the present invention. The present invention also relates to use of a polypeptide of the present invention in the production of a therapeutic or preventive agent for immune inflammatory diseases. Furthermore, the present invention relates to a polypeptide of the present invention for use in the method for treating or preventing immune inflammatory diseases of the present invention.

[0103] The present invention relates to an activation inhibitor for B cells, mast cells, dendritic cells, and/or basophils, which comprises a polypeptide of the present invention. Furthermore, the present invention relates to a method of inhibiting activation of B cells, mast cells, dendritic cells, and/or basophils, which comprises administering a polypeptide of the present invention to a subject. The present invention also relates to a kit for use in the method of inhibiting activation of B cells, mast cells, dendritic cells, and/or basophils, which comprises a polypeptide of the present invention. The present invention relates to a use of a polypeptide of the present invention in producing activation inhibitors for B cells, mast cells, dendritic cells, and/or basophils. The present invention also relates to a polypeptide of the present invention for use in the method of the present invention of inhibiting activation of B cells, mast cells, dendritic cells, and/or basophils.

[0104] Furthermore, the present invention relates to a therapeutic agent for diseases in which a protein necessary for an organism is deficient, wherein the agent comprises a polypeptide of the present invention. The present invention also relates to a method for treating diseases in which a protein necessary for an organism is deficient, which comprises administering a polypeptide of the present invention to a subject. Furthermore, the present invention relates to a kit for use in the method of the present invention for treating diseases in which a protein necessary for an organism is deficient, wherein the kit comprises a polypeptide of the present invention. The present invention relates to use of a polypeptide of the present invention in producing a therapeutic agent for diseases in which a protein necessary for an organism is deficient. The present invention also relates to a polypeptide of the present invention for use in the method of the present invention for treating diseases in which a protein necessary for an organism is deficient.

[0105] In addition, the present invention relates to an agent for suppressing virus proliferation, which comprises a polypeptide of the present invention. The present invention also relates to a method of suppressing virus proliferation, which comprises administering a polypeptide of the present invention to a subject. Furthermore, the present invention relates to a kit of the present invention for use in the method of suppressing virus proliferation, wherein the kit comprises a polypeptide of the present invention. The present invention relates to use of a polypeptide of the present invention in producing an agent for suppressing virus proliferation. The present invention also relates to a polypeptide of the present invention for use in the method of the present invention of suppressing virus proliferation.

[0106] The present invention provides Fc region variants whose binding activities to all activating FcγRs, in particular FcγRIIa (R type), have been reduced, while their FcγRIIb binding activity is maintained, when compared to those of the naturally-occurring IgG Fc region. By using polypeptides containing the Fc region variants, it is possible to enhance the inflammatory immune response-suppressing signals produced by phosphorylation of ITIM of FcγRIIb, under conditions where the property of eliminating immune complexes via FcγRIIb is maintained to a similar degree as in a naturally-occurring IgG. Furthermore, by conferring an Fc region with the property of selectively binding to FcγRIIb, it may be possible to suppress anti-antibody production. Furthermore, by reducing binding to activating FcγRs, platelet activation mediated by interaction between FcγRIIa on the platelet and the immune complex, and dendritic cell activation by crosslinking of activating FcγRs can be avoided.

[0126] The present invention provides Fc region variants that can lower binding activities to all activating FcγRs, in particular FcγRIIa (R type), while maintaining FcγRIIb-binding activity, when compared to a polypeptide containing a native IgG antibody Fc region, and polypeptides comprising the Fc region variants.

[0127] More specifically, the invention provides Fc region variants comprising an amino acid sequence in which the amino acid alteration at position 238 according to EU numbering is combined with other specific amino acid alterations, and polypeptides comprising the Fc region variants. Furthermore, the present invention provides methods of introducing the amino acid alterations into an Fc region for decreasing its binding activities to all activating FcγRs, in particular FcγRIIa (R type), while maintaining its FcγRIIb-binding activity in comparison to those of a polypeptide containing a native IgG antibody Fc region; and methods of introducing the amino acid alterations into an Fc region for producing polypeptides comprising an Fc region variant with reduced binding activities to all activating FcγRs, in particular FcγRIIa (R type), and maintained FcγRIIb-binding activity in comparison to those of polypeptides containing the native IgG Fc region. The invention also provides Fc region variants in which the amino acid alterations have been introduced into the Fc region, and polypeptides that have binding activity to a disease-causing antigen present in the plasma in soluble form, and comprise an antigen-binding domain whose binding activity to the antigen changes according to the ion concentration condition, and methods of using the polypeptides for promoting elimination of the antigen in the plasma.

[0128] “Polypeptides” of the present invention generally refers to peptides or proteins approximately ten amino acids or more in length. Furthermore, they are generally polypeptides derived from organisms, but are not particularly limited, and for example, they may be polypeptides comprising an artificially designed sequence. Furthermore, they may be any of naturally-occurring polypeptides, synthetic polypeptides, recombinant polypeptides, or such.

[0129] Preferred examples of the polypeptides of the present invention include antibodies. More preferred examples include naturally-occurring IgGs, particularly naturally-occurring human IgGs. “Naturally-occurring (native) IgGs” refers to polypeptides belonging to a class of antibodies practically encoded by immunoglobulin gamma genes and comprising an amino acid sequence identical to those of IgGs found in nature. For example, a naturally-occurring human IgG means a naturally-occurring human IgG1, naturally-occurring human IgG2, naturally-occurring human IgG3, naturally-occurring human IgG4, or such. Naturally-occurring IgGs also include mutants spontaneously produced from them.

[0130] While an IgK (Kappa, κ chain), IgL1, IgL2, IgL3, IgL6, and IgL7 (Lambda, λ chain)-type constant region is present in the antibody light chain constant region, it may be any light chain constant region. For the human IgK (Kappa) constant region and human IgL7 (Lambda) constant region, a plurality of allotype sequences due to genetic polymorphism are described in “Sequences of proteins of immunological interest”, NIH Publication No. 91-3242, and any of them may be used in the present invention. Furthermore, in the present invention, a light chain constant region may be a light chain constant region that has been altered with amino acid substitutions, additions, deletions, insertions, and/or modifications or such. For the antibody Fc region, for example, Fc regions of the IgA1, IgA2, IgD, IgE, IgG1, IgG2, IgG3, IgG4, and IgM types exist. For example, a human IgG antibody Fc region can be used as the antibody Fc region of the present invention, and human IgG1 antibody Fc regions are preferred. Fc regions that can be used as an Fc region of the present invention are, for example, those derived from naturally-occurring IgG constant regions, or specifically, a constant region derived from naturally-occurring human IgG1 (SEQ ID NO: 31), a constant region derived from naturally-occurring human IgG2 (SEQ ID NO: 32), a constant region derived from naturally-occurring human IgG3 (SEQ ID NO: 33), and a constant region derived from naturally-occurring human IgG4 (SEQ ID NO: 34). FIG. 18 shows the constant region sequences of the naturally-occurring IgG1, IgG2, IgG3, and IgG4. Constant regions of naturally-occurring IgGs also include mutants spontaneously produced from them. For the constant regions of human IgG1, human IgG2, human IgG3, and human IgG4 antibodies, a plurality of allotype sequences due to genetic polymorphism are described in “Sequences of proteins of immunological interest”, NIH Publication No. 91-3242, and any of them may be used in the present invention. In particular, for the human IgG1 sequence, the amino acid sequence at positions 356 to 358 (EU numbering) may be either DEL or EEM.

[0131] “Fcγ receptors” (herein, referred to as Fcγ receptors, FcγR or FcgR) refers to receptors that may bind to the Fc region of IgG1, IgG2, IgG3, and IgG4 monoclonal antibodies, and practically means any member of the family of proteins encoded by the Fcγ receptor genes. In humans, this family includes FcγRI (CD64) including isoforms FcγRIa, FcγRIb, and FcγRIc; FcγRII (CD32) including isoforms FcγRIIa (including allotypes H131 (type H) and R131 (type R)), FcγRIIb (including FcγRIIb-1 and FcγRIIb-2), and FcγRIIc; and FcγRIII (CD16) including isoforms FcγRIIIa (including allotypes V158 and F158), and FcγRIIIb (including allotypes FcγRIIIb-NA1 and FcγRIIIb-NA2), and any human FcγRs, FcγR isoforms or allotypes yet to be discovered, but is not limited thereto. FcγRIIb1 and FcγRIIb2 have been reported as splicing variants of human FcγRIIb. In addition, a splicing variant named FcγRIIb3 has been reported (J. Exp. Med, 1989, 170: 1369). In addition to these splicing variants, human FcγRIIb includes all splicing variants registered in NCBI, which are NP_—001002273.1, NP_—001002274.1, NP_—001002275.1, NP_—001177757.1, and NP_—003992.3. Furthermore, human FcγRIIb includes every previously-reported genetic polymorphism, as well as FcγRIIb (Arthritis Rheum, 2003, 48: 3242-52; Hum Mol Genet, 2005, 14: 2881-92; and Arthritis Rheum. 2002 May; 46(5): 1242-54), and every genetic polymorphism that will be reported in the future. The FcγR includes human, mouse, rat, rabbit, and monkey-derived FcγRs but is not limited thereto, and may be derived from any organism. Mouse FcγRs include FcγRI (CD64), FcγRII (CD32), FcγRIII (CD16), and FcγRIII-2 (CD16-2), and any mouse FcγRs, or FcγR isoforms or allotypes yet to be discovered, but are not limited thereto. Favorable examples of such Fcγ receptors include human FcγRI (CD64), FcγRIIA (CD32), FcγRIIB (CD32), FcγRIIIA (CD16), and/or FcγRIIIB (CD16).

[0132] The polynucleotide sequence and amino acid sequence of FcγRI are set forth in SEQ ID NOs: 35 (NM_—000566.3) and 36 (NP_—000557.1), respectively;

the polynucleotide sequence and amino acid sequence of FcγRIIA are set forth in SEQ ID NOs: 37 (BC020823.1) and 38 (AAH20823.1), respectively;
the polynucleotide sequence and amino acid sequence of FcγRIIB are set forth in SEQ ID NOs: 39 (BC146678.1) and 40 (AAI46679.1), respectively;
the polynucleotide sequence and amino acid sequence of FcγRIIIA are set forth in SEQ ID NOs: 41 (BC033678.1) and 42 (AAH33678.1), respectively; and
the polynucleotide sequence and amino acid sequence of FcγRIIIB are set forth in SEQ ID NOs 43 (BC128562.1) and 44 (AAI28563.1), respectively (the RefSeq Registration number is indicated inside the parentheses).

[0133] In FcγRIIa, there are two allotypes: one where the amino acid at position 131 of FcγRIIa is histidine (type H) and the other where this amino acid is substituted with arginine (type R) (J. Exp. Med, 172: 19-25, 1990).

[0134] In FcγRI (CD64) including FcγRIa, FcγRIb, and FcγRIc, and in FcγRIII (CD16) including isoform FcγRIIIa (including allotypes V158 and F158), the a chain that binds to the Fc portion of IgG is associated with the common γ chain having ITAM responsible for transducing activation signals inside cells. FcγRIIIb (including allotypes FcγRIIIb-NA1 and FcγRIIIb-NA2) is a GPI anchor protein. Meanwhile, the cytoplasmic domain of FcγRII (CD32) itself which includes isoforms FcγRIIa (including allotypes H131 and R131) and FcγRIIc, contains ITAM. These receptors are expressed on many immune cells such as macrophages, mast cells, and antigen-presenting cells. The activation signals transduced when these receptors bind to the Fc portion of IgG enhance the phagocytic ability of macrophages, inflammatory cytokine production, mast cell degranulation, and promotion of the function of antigen-presenting cells. Fcγ receptors having the ability to transduce the activation signals as described above are also referred to as activating Fcγ receptors in the present invention.

[0135] Meanwhile, the intracytoplasmic domain of FcγRIIb (including FcγRIIb-1 and FcγRIIb-2) itself contains ITIM responsible for transduction of inhibitory signals. The crosslinking between FcγRIIb and B cell receptor (BCR) on B cells suppresses the activation signals from BCR, which results in suppression of antibody production via BCR. The crosslinking of FcγRIII and FcγRIIb on macrophages suppresses the phagocytic ability and the ability to produce inflammatory cytokines. Fcγ receptors having the ability to transduce the inhibitory signals as described above are also referred to as inhibitory Fcγ receptors in the present invention.

[0136] In the present invention, an “Fc region variant” refers to an Fc region in which at least one amino acid of the present invention has been altered into another amino acid in an Fc region not introduced with the amino acid alteration of the present invention. Herein, an Fc region in which “at least one amino acid has been altered to another amino acid” includes an Fc region into which the amino acid alteration has been introduced, and an Fc region comprising an amino acid sequence identical to that of the Fc region comprising the amino acid alteration.

[0137] “Naturally-occurring IgGs (Native IgGs)” refers to polypeptides belonging to a class of antibodies substantially encoded by immunoglobulin gamma genes and comprising an amino acid sequence identical to those of IgGs found in nature. For example, a native human IgG refers to a native human IgG1, native human IgG2, native human IgG3, native human IgG4, or such. Native IgGs also include mutants spontaneously produced therefrom, and IgGs introduced with alterations that do not substantially affect the FcγR-binding activities.

[0138] The Fc region of a native IgG means an Fc region comprising an amino acid sequence identical to that of the Fc region derived from an IgG found in nature. The heavy chain constant region of the native IgG is shown in FIG. 18 (SEQ ID NOs: 31 to 34), and for example, it refers to Fc regions in the heavy chain constant regions derived from native human IgG1, Fc regions in the heavy chain constant regions derived from native human IgG2, Fc regions in the heavy chain constant regions derived from native human IgG3, and Fc regions in the heavy chain constant regions derived from native human IgG4. The Fc regions of native IgGs also include mutants spontaneously produced therefrom and Fc regions introduced with alterations that do not substantially affect the FcγR-binding activities.

[0139] In the present invention, whether or not the binding activity towards each type of FcγR is enhanced, or maintained or decreased in a polypeptide comprising an Fc region variant or an Fc region variant of the present invention can be determined, for example, by observing whether there is a decrease or an increase in the dissociation constant (KD) value obtained from the results of sensorgram analysis, where various FcγRs are subjected to interaction as an analyte with antibodies immobilized onto the sensor chips or captured onto the sensor chips using Protein A, Protein L, Protein A/G, Protein G, anti-lamda chain antibodies, anti-kappa chain antibodies, antigen peptides, antigen proteins, or such using BIACORE which is an interaction analyzer that utilizes the surface plasmon resonance (SPR) phenomena, as shown in the Examples or Reference Examples. Alternatively, it can also be determined by observing whether there is an increase or a decrease in the value obtained by dividing the amount of change in the resonance unit (RU) value on the sensorgram before and after various types of FcγRs are subjected to interaction as an analyte with antibodies immobilized onto the sensor chips or captured onto the sensor chips using Protein A, Protein L, Protein A/G, Protein G, anti-lamda chain antibodies, anti-kappa chain antibodies, antigen peptides, antigen proteins, or such, by the amount of change of resonance units (RU) before and after antibodies are immobilized or captured onto the sensor chip. Furthermore, it can be determined by observing an increase or a decrease in the dissociation constant (KD) values obtained from sensorgram analysis, where a sample such as an antibody to be evaluated is subjected to interaction as an analyte using a sensor chip onto which FcγR is immobilized directly or via an anti-tag antibody. Alternatively, it can be determined by observing whether the amount of change in sensorgram values increases or decreases before and after a sample such as an antibody to be evaluated is subjected to interaction as an analyte with the sensor chip onto which FcγR is immobilized directly or via an anti-tag antibody.

[0140] Specifically, the binding activity of an Fc region variant towards an Fcγ receptor can be measured by the Amplified Luminescent Proximity Homogeneous Assay (ALPHA) screening, the BIACORE method which utilizes the surface plasmon resonance (SPR) phenomena, or such, in addition to ELISA or fluorescence activated cell sorting (FACS) (Proc. Natl. Acad. Sci. USA (2006) 103 (11): 4005-4010).

[0141] ALPHA screening is performed by ALPHA technology which uses two beads, a donor and an acceptor, based on the following principles. Luminescent signals are detected only when molecules bound to donor beads physically interact with molecules bound to the acceptor beads, and the two beads are in close proximity to each other. Laser-excited photosensitizer in the donor beads converts ambient oxygen to excited-state singlet oxygen. Singlet oxygen is dispersed around the donor beads, and when it reaches the adjacent acceptor beads, chemiluminescent reaction is induced in the beads, and light is ultimately emitted. When the molecules bound to the donor beads do not interact with the molecules bound to the acceptor beads, the chemiluminescent reaction does not take place because singlet oxygen produced by the donor beads does not reach the acceptor beads.

[0142] For example, a biotinylated polypeptide complex is bound to the donor beads, and Fcγ receptor tagged with glutathione S transferase (GST) is linked to the acceptor beads. In the absence of a competing polypeptide complex comprising an Fc region variant, the polypeptide complex comprising a wild-type Fc region interacts with the Fcγ receptor and produces 520-620 nm signals. The polypeptide complex comprising an untagged mutant Fc region competes with the polypeptide complex comprising a wild-type Fc region for interaction with the Fcγ receptor. Relative binding activities can be determined by quantifying the decrease in fluorescence observed as a result of the competition. Biotinylation of polypeptide complexes such as antibodies using Sulfo-NHS-biotin and such is well known. The method of expressing the Fcγ receptor and GST in a cell carrying a fusion gene produced by fusing a polynucleotide encoding the Fcγ receptor in frame with a polynucleotide encoding GST in an expressible vector, and performing purification using a glutathione column is appropriately adopted as a method for tagging an Fcγ receptor with GST. The obtained signals are preferably analyzed, for example, by fitting them to a one-site competition model which uses a non-linear regression analysis using software such as GRAPHPAD PRISM (GraphPad, San Diego).

[0143] One of the substances (the ligand) in observation of an interaction is immobilized onto a gold thin film on a sensor chip, and by shining light from the reverse side of the sensor chip so that total reflection takes place at the interface between the gold thin film and glass, a portion of reduced reflection intensity is formed in part of the reflected light (SPR signal). When the other one of the substances (the analyte) in observation of an interaction is made to flow on the sensor chip surface and the ligand binds to the analyte, the mass of the immobilized ligand molecule increases and the refractive index of the solvent on the sensor chip surface changes. The position of the SPR signal shifts as a result of this change in refractive index (on the other hand, the signal position returns when this binding dissociates). The Biacore system indicates the amount of shift mentioned above, or more specifically the time variable of mass by plotting the change in mass on the sensor chip surface on the ordinate as the measurement data (sensorgram). The amount of analyte bound to the ligand trapped on the sensor chip surface is determined from the sensorgram. Kinetic parameters such as association rate constants (ka) and dissociation rate constants (kd) are determined from the curves of the sensorgram, and the dissociation constants (KD) are determined from the ratio of these constants. In the BIACORE method, a method for measuring inhibition is preferably used. An example of the method for measuring inhibition is described in Proc. Natl. Acad. Sci USA (2006) 103 (11): 4005-4010.

[0144] An Fc region whose FcγRIIb-binding activity is maintained or a polypeptide comprising such an Fc region refers to a polypeptide that binds to FcγRIIb with a binding activity equivalent to or substantially unchanged from the parent polypeptide when assay is performed with substantially the same amount of a polypeptide containing the Fc region of the native IgG (also referred to as a polypeptide containing the parent Fc region or a parent polypeptide) and a polypeptide comprising the amino acid alterations of the present invention in the Fc region (a polypeptide containing the Fc region variant). Specifically, it refers to an Fc region variant that maintains at least 55.5% of the FcγRIIb-binding property of the polypeptide containing the parent Fc region.

[0145] An Fc region whose binding activities to activating FcγRs have been decreased, lowered, or attenuated, or a polypeptide containing such an Fc region refers to an Fc region variant or a polypeptide containing the Fc region variant which binds to activating FcγRs with binding activities substantially weaker than those of polypeptides containing the parent Fc region when assay is performed with substantially the same amount of a polypeptide containing the Fc region of the native IgG (also referred to as a polypeptide containing the parent Fc region or a parent polypeptide) and a polypeptide which includes amino acid alterations of the present invention in the Fc region (a polypeptide containing an Fc region variant).

[0146] Whether or not an Fc region variant of the present invention maintains the FcγRIIb-binding activity of the Fc region of a native IgG can be determined by comparing the KD value for FcγRIIb of the polypeptide comprising the Fc region variant of the present invention with the KD value for FcγRIIb of the polypeptide containing the Fc region of a native IgG, which can be determined, for example, according to the above-mentioned examples. Specifically, when the KD value for the polypeptide containing an Fc region variant of the present invention is a value equivalent to or less than that of a polypeptide containing the parent Fc region, the polypeptide containing the Fc region variant of the present invention can be determined to have maintained its FcγRIIb-binding activity in comparison to a polypeptide containing the parent Fc region variant. Whether or not the activating FcγR-binding activity of an Fc region variant of the present invention is decreased in comparison to that of a native IgG Fc region can be determined in a similar manner, for example, by comparing the KD value for an activating FcγR of the polypeptide comprising the Fc region variant of the present invention with the KD value for the activating FcγR of the polypeptide containing the Fc region of a native IgG, as determined according to the above-mentioned examples. Specifically, when the KD value of the polypeptide comprising the Fc region variant of the present invention is enhanced compared to that of the polypeptide comprising the parent Fc region, the polypeptide comprising the Fc region variant of the present invention can be determined to have a reduced binding activity for activating FcγR in comparison to that of the polypeptide comprising the parent Fc region variant. In particular, since the FcγRIIa (R type)-binding activity is more easily correlated with the FcγRIIb-binding activity than with binding activities for other activating FcγRs, finding amino acid alterations that can decrease the FcγRIIa (R type)-binding activity while maintaining the FcγRIIb-binding activity is the most difficult task in selectively decreasing the binding activities for activating FcγRs other than FcγRIIb.

[0147] An equivalent or maintained FcγRIIb-binding activity means that, for example, in the KD values determined by the measurement method described above, the KD ratio of [KD value for FcγRIIb of the polypeptide comprising the parent Fc region]/[KD value for FcγRIIb of the polypeptide comprising the Fc region variant] is preferably at least 0.75, more preferably at least 0.8, even more preferably at least 0.9. Furthermore, a value of about 5 is sufficient for the KD ratio, and a higher value is not necessary for determining that the FcγRIIb-binding activity is equivalent or maintained.

[0148] Decrease, reduction or attenuation of binding activities for activating FcγRs means that, for example, in the KD value determined by the above-mentioned measurement method, the KD ratio for [KD value for an activating FcγR of the polypeptide comprising the parent Fc region]/[KD value for the activating FcγR of the polypeptide comprising the Fc region variant] is preferably 0.2 or less, more preferably 0.15 or less, or more preferably 0.1 or less.

[0149] In particular, since the extracellular region sequences of FcγRIIa and FcγRIIb have 93% identity and their structures are extremely similar, for the FcγRIIaR-binding activity, which is difficult to decrease while maintaining the FcγRIIb-binding activity, the KD ratio for [KD value for FcγRIIaR of the polypeptide comprising the parent Fc region]/[KD value for FcγRIIaR of the polypeptide comprising the Fc region variant] is preferably 0.1 or less, and more preferably 0.05.

[0150] Furthermore, whether or not the binding activities of the polypeptides of the present invention towards various FcγRs were maintained, enhanced, or decreased can be determined from the increase or decrease in the amount of binding of the various FcγRs to the polypeptides of the present invention, which were determined according to the examples described above. Here, the amount of binding of the various FcγRs to the polypeptides refers to values obtained by determining the difference in the RU values of sensorgrams that changed before and after interaction of various FcγRs as the analyte with each polypeptide, and dividing them by differences in the RU values of sensorgrams that changed before and after capturing polypeptides to the sensor chips.

[0151] Furthermore, an Fc region whose selectivity for FcγRIIb has been improved or a polypeptide comprising such an Fc region, or an Fc region whose binding activities to activating FcγRs have been selectively reduced or a polypeptide comprising such an Fc region refers to an Fc region whose binding activities to activating FcγRs have been decreased, lowered, or attenuated, while maintaining the FcγRIIb-binding activity, or a polypeptide comprising such an Fc region.

[0152] Fc region variants of the present invention are not particularly limited in terms of their KD values (mol/L) for FcγRIIb and activating FcγRs; however, for example, the value for FcγRIIb may be 7.0×10⁻⁶ or less, preferably 6.0×10⁻⁶ or less, or more preferably 5.0×10⁻⁶ or less, and the values for activating FcγRs may be 2.5×10⁻⁹ or higher, preferably 3.0×10⁻⁹ or higher, or more preferably 3.5×10⁻⁹ or higher, and the value for FcγRIIa (R type) in particular is preferably 2.0×10⁻⁵ or higher.

[0153] An “Fc region” refers to a fragment consisting of a hinge portion or a part thereof, and CH2 and CH3 domains in an antibody molecule. According to EU numbering (herein, also referred to as EU INDEX) (see FIG. 18), an IgG-class Fc region refers to, for example, the region from cysteine at position 226 to the C terminus, or from proline at position 230 to the C terminus, but is not limited thereto.

[0154] The Fc region may be obtained suitably by re-eluting the fraction adsorbed onto a protein A column and a protein G column after partially digesting IgG1, IgG2, IgG3, IgG4 monoclonal antibodies or such using a protease such as pepsin. Such protease is not particularly limited as long as it can digest a full-length antibody so as to produce restrictively Fab and F(ab′)₂ by appropriately setting the enzyme reaction conditions such as pH, and examples include pepsin and papain.

[0155] The present invention provides an Fc region variant which comprises amino acid alterations that combine an alteration of the amino acid at position 238 according to EU numbering to another amino acid with alteration of any one of the amino acids of (a) to (k) below to another amino acid in the Fc region of human IgG (IgG1, IgG2, IgG3, and IgG4). Introducing the alterations into an Fc region can provide a polypeptide comprising an Fc region variant with decreased binding activities to all activating FcγRs, in particular FcγRIIa (R type), while maintaining the FcγRIIb-binding activity as compared to those of a polypeptide comprising the Fc region of a native IgG:

(a) amino acid at position 235 of the Fc region according to EU numbering;
(b) amino acid at position 237 of the Fc region according to EU numbering;
(c) amino acid at position 241 of the Fc region according to EU numbering;
(d) amino acid at position 268 of the Fc region according to EU numbering;
(e) amino acid at position 295 of the Fc region according to EU numbering;
(f) amino acid at position 296 of the Fc region according to EU numbering;
(g) amino acid at position 298 of the Fc region according to EU numbering;
(h) amino acid at position 323 of the Fc region according to EU numbering;
(i) amino acid at position 324 of the Fc region according to EU numbering;
(j) amino acid at position 330 of the Fc region according to EU numbering; and
(k) at least two amino acids selected from (a) to (j).

[0156] A combination of at least two amino acids selected in the above-mentioned (k) is not particularly limited as long as binding activities to all activating FcγRs are decreased while the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the native IgG Fc region, but the following combinations (1) to (3) are preferred:

(1) amino acids at positions 241, 268, 296, and 324 of the Fc region according to EU numbering;
(2) amino acids at positions 237, 241, 296, and 330 of the Fc region according to EU numbering; and
(3) amino acids at positions 235, 237, 241, and 296 of the Fc region according to EU numbering.

[0157] The amino acids selected to be present after alteration are not particularly limited as long as binding activities to all activating FcγRs are decreased while the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the Fc region of a native IgG, but are preferably Asp at amino acid position 238, Phe at amino acid position 235, Gln or Asp at amino acid position 237, Met or Leu at amino acid position 241, Pro at amino acid position 268, Met or Val at amino acid position 295, Glu, His, Asn, or Asp at amino acid position 296, Ala or Met at amino acid position 298, Ile at amino acid position 323, Asn or His at amino acid position 324, and His or Tyr at amino acid position 330 according to EU numbering. Furthermore, the amino acids selected to be present after the alteration with regard to (1) to (3) described above are preferably:

(1) Met at amino acid position 241, Pro at amino acid position 268, Glu at amino acid position 296, and His at amino acid position 324 of the Fc region according to EU numbering;
(2) Gln or Asp at amino acid position 237, Met at amino acid position 241, Glu at amino acid position 296, and His at amino acid position 330 of the Fc region according to EU numbering; and
(3) Phe at amino acid position 235, Gln or Asp at amino acid position 237, Met at amino acid position 241, and Glu at amino acid position 296 of the Fc region according to EU numbering.

[0158] Furthermore, the present invention provides an Fc region variant comprising amino acid alterations which combine alterations of the amino acids at positions 238 and 271 of the human IgG Fc region according to EU numbering to other amino acids, with alteration of any one of the amino acids of (a) to (h) below to another amino acid. Introducing the alterations into the Fc region can provide a polypeptide comprising an Fc region variant with decreased binding activities to all activating FcγRs, in particular FcγRIIa (R type), while maintaining the FcγRIIb-binding activity in comparison to those of a polypeptide comprising the Fc region of a native IgG.

(a) amino acid at position 234 of the Fc region according to EU numbering;
(b) amino acid at position 235 of the Fc region according to EU numbering;
(c) amino acid at position 236 of the Fc region according to EU numbering;
(d) amino acid at position 237 of the Fc region according to EU numbering;
(e) amino acid at position 239 of the Fc region according to EU numbering;
(f) amino acid at position 265 of the Fc region according to EU numbering;
(g) amino acid at position 267 of the Fc region according to EU numbering; and
(h) amino acid at position 297 of the Fc region according to EU numbering.

[0159] Amino acid alterations to be combined with alterations of the amino acids at positions 238 and 271 according to EU numbering to other amino acids may further include alterations of other amino acids in addition to the amino acids of (a) to (h) described above. Such amino acid combinations are not particularly limited, but a combination of alterations selected from (1) to (3) below is preferred:

(1) amino acids at positions 233, 238, 264, 267, 268, and 271 of the Fc region according to EU numbering;
(2) amino acids at positions 233, 237, 238, 264, 267, 268, 271, 296, 297, 330, and 396 of the Fc region according to EU numbering; and
(3) amino acids at positions 233, 238, 264, 267, 268, 271 and 296 of the Fc region according to EU numbering.

[0160] The amino acids selected to be present after alteration are not particularly limited as long as binding activities to all activating FcγRs are decreased while the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the Fc region of a native IgG, but are preferably Asp at amino acid position 238, Gly at amino acid position 271, Ala, His, Asn, Lys, or Arg at amino acid at 234, Ala at amino acid position 235, Gln at amino acid position 236, Arg or Lys at amino acid position 237, Lys at amino acid position 239, Lys, Asn, Arg, Ser, or Val at amino acid position 265, Lys, Arg, or Tyr at amino acid position 267, and Ala at amino acid position 297 according to EU numbering.

[0161] Furthermore, the amino acids selected to be present after the alterations in (1) to (3) described above are preferably:

(1) Asp at amino acid position 233, Asp at amino acid position 238, Ile at amino acid position 264, Arg at amino acid position 267, Glu at amino acid position 268, and Gly at amino acid position 271 of the Fc region according to EU numbering;
(2) Asp at amino acid position 233, Asp at amino acid position 237, Asp at amino acid position 238, Ile at amino acid position 264, Ala at amino acid position 267, Glu at amino acid position 268, Gly at amino acid position 271, Asp at amino acid position 296, Ala at amino acid position 297, Arg at amino acid position 330, and Met at amino acid position 396 of the Fc region according to EU numbering; and
(3) Asp at amino acid position 233, Asp at amino acid position 238, Ile at amino acid position 264, Arg at amino acid position 267, Pro at amino acid position 268, Gly at amino acid position 271, and Glu at amino acid position 296 of the Fc region according to EU numbering.

[0162] Furthermore, the present invention provides an Fc region variant comprising alterations of the amino acids at positions 238, 271, 327, 330, and 331 of the human IgG Fc region according to EU numbering to other amino acids. The present invention also provides an Fc region variant comprising amino acid alterations, where the variant further comprises alteration of a combination of the amino acids of any one of (a) to (e) below to other amino acids. Introducing the alterations into the Fc region can provide a polypeptide comprising an Fc region variant with decreased binding activities to all activating FcγRs, in particular FcγRIIa (R type), while maintaining FcγRIIb-binding activity as compared to those of a polypeptide comprising the Fc region of a native IgG.

(a) the amino acid at position 233 of the Fc region according to EU numbering;
(b) the amino acid at position 237 of the Fc region according to EU numbering;
(c) the amino acid at position 264 of the Fc region according to EU numbering;
(d) the amino acid at position 267 of the Fc region according to EU numbering; and
(e) the amino acid at position 268 of the Fc region according to EU numbering.

[0163] Amino acid alterations to be combined with the alterations of amino acids at positions 238 and 271 according to EU numbering to other amino acids may include alterations of other amino acids in addition to the amino acids of (a) to (e) described above. Such amino acid combinations are not particularly limited, but a combination of alterations selected from (1) to (4) below is preferred.

(1) amino acids at positions 237, 238, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering;
(2) amino acids at positions 233, 237, 238, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering;
(3) amino acids at positions 238, 267, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering; and
(4) amino acids at positions 238, 264, 267, 271, 327, 330, and 331 of the Fc region according to EU numbering.

[0164] The amino acids selected to be present after alteration are not particularly limited as long as binding activities to all activating FcγRs are decreased while the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the Fc region of a native IgG, but are preferably Asp at amino acid position 238, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, Ser at amino acid position 331, Asp at amino acid position 233, Asp at amino acid position 237, Ile at amino acid position 264, Ala at amino acid position 267, and Asp or Glu at amino acid position 268 according to EU numbering.

[0165] Furthermore, the amino acids selected to be present after the alterations in (1) to (4) described above are preferably:

(1) Asp at amino acid position 237, Asp at amino acid position 238, Asp or Glu at amino acid position 268, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid at position 330, and Ser at amino acid position 331 of the Fc region according to EU numbering;
(2) Asp at amino acid position 233, Asp at amino acid position 237, Asp at amino acid position 238, Asp at amino acid position 268, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, and Ser at amino acid position 331 of the Fc region according to EU numbering;
(3) Asp at amino acid position 238, Ala at amino acid position 267, Glu at amino acid position 268, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, and Ser at amino acid position 331 of the Fc region according to EU numbering; and
(4) Asp at amino acid position 238, Ile at amino acid position 264, Ala at amino acid position 267, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, and Ser at amino acid position 331 of the Fc region according to EU numbering.

[0166] In the present invention, at least another different alteration can be made to the Fc region in addition to these alterations. The alteration is not particularly limited as long as it decreases the binding activities to activating FcγRs while maintaining the FcγRIIb-binding activity.

[0167] Examples of such an alteration include those that decrease the complement-binding activity. Specific examples include amino acid alteration at position 322 of the Fc region according to EU numbering, or a combination of amino acid alterations at positions 327, 330, and 331 of the Fc region according to EU numbering. The amino acids selected to be present after alteration are not particularly limited as long as the complement-binding activity is decreased while binding activities to all activating FcγRs are decreased and the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the Fc region of a native IgG, but are preferably Ala or Glu at amino acid position 322, Gly at amino acid position 327, Ser at amino acid position 330, and Ser at amino acid position 331 according to EU numbering.

[0168] In the present invention, whether the complement-binding activity of the Fc region variant or the polypeptide comprising the Fc region variant of the present invention is decreased can be confirmed by a method similar to the method described above for confirming whether the FcγR-binding activities have been decreased. Specifically, for example, the determination can be made by observing whether there is an increase in the dissociation constant (KD) value obtained from the results of sensorgram analysis, where the complement is interacted as an analyte with antibodies to be evaluated which are immobilized onto the sensor chips or captured onto the sensor chips using Protein A, Protein L, Protein A/G, Protein G, anti-lamda chain antibodies, anti-kappa chain antibodies, antigen peptides, antigen proteins, or such using BIACORE, which is an interaction analyzer that utilizes the surface plasmon resonance (SPR) phenomena, as shown in the Examples. Alternatively, it can also be determined by observing whether there is an increase in the value obtained by dividing the amount of change in the resonance unit (RU) value on the sensorgram before and after the complement is subjected to interaction as an analyte with antibodies to be evaluated immobilized onto the sensor chips or captured onto the sensor chips using Protein A, Protein L, Protein A/G, Protein G, anti-lamda chain antibodies, anti-kappa chain antibodies, antigen peptides, antigen proteins, or such, by the amount of change of resonance units (RU) before and after the antibodies are immobilized or captured onto the sensor chip. Furthermore, it can be determined by observing whether there is an increase in the dissociation constant (KD) values obtained from sensorgram analysis, where a sample such as an antibody to be evaluated is subjected to interaction as an analyte using a sensor chip onto which a complement has been immobilized directly or via an anti-tag antibody. Alternatively, it can be determined by observing whether the amount of change in sensorgram values increases or not before and after a sample such as an antibody to be evaluated is subjected to interaction as an analyte with the sensor chip onto which a complement is immobilized directly or via an anti-tag antibody. Otherwise, it can be determined by evaluating the amount of binding by ELISA which involves adding the complement to a plate onto which an antibody to be evaluated has been immobilized directly or via an antigen, and then adding an anti-human C1q antibody labeled with peroxidase or such.

[0169] Amino acid alterations carried out for other purposes may also be combined into the polypeptide comprising an Fc region variant of the present invention. For example, amino acid substitutions that improve the FcRn-binding activity (J. Immunol. 2006 Jan. 1; 176(1): 346-56; J Biol Chem. 2006 Aug. 18; 281(33): 23514-24; Int. Immunol. 2006 December; 18(12): 1759-69; Nat Biotechnol. 2010 February; 28(2): 157-9; WO 2006/019447; WO 2006/053301; and WO 2009/086320), and amino acid substitutions for improving antibody heterogeneity or stability (WO 2009/041613) may be introduced. Alternatively, polypeptides produced by conferring polypeptides comprising an Fc region variant of the present invention with the property of promoting antigen elimination, which are described in WO 2011/122011 or PCT/JP2011/072550, and polypeptides conferred with the property for repeatedly binding to multiple antigen molecules, which are described in WO 2009/125825 or PCT/JP2011/077619, are also included in the present invention. Alternatively, with the objective of increasing plasma retention, amino acid alterations that decrease the pI of the constant region (WO 2012/016227) may be combined into a polypeptide comprising an Fc region variant of the present invention. Otherwise, with the objective of conferring binding ability to other antigens, the amino acid alterations in CH3 described in EP1752471 and EP1772465 may be combined into a polypeptide comprising an Fc region variant of the present invention.

[0170] When a polypeptide comprising an Fc region variant of the present invention comprises an antigen-binding domain such as in an antibody, amino acid alteration for changing its antigen-binding activity according to the ion concentration condition can be combined to enhance the effect of the polypeptide to eliminate antigens from plasma.

[0171] Herein, an “antigen-binding domain” may be of any structure as long as it binds to an antigen of interest. Such domains preferably include, for example:

antibody heavy-chain and light-chain variable regions;
a module of about 35 amino acids called A domain which is contained in the in vivo cell membrane protein Avimer (WO 2004/044011, WO 2005/040229);
Adnectin containing the 10Fn3 domain which binds to the protein moiety of fibronectin, a glycoprotein expressed on cell membrane (WO 2002/032925);
Affibody which is composed of a 58-amino acid three-helix bundle based on the scaffold of the IgG-binding domain of Protein A (WO 1995/001937);
Designed Ankyrin Repeat proteins (DARPins) which are a region exposed on the molecular surface of ankyrin repeats (AR) having a structure in which a subunit consisting of a turn comprising 33 amino acid residues, two antiparallel helices, and a loop is repeatedly stacked (WO 2002/020565);
Anticalins and such, which are domains consisting of four loops that support one side of a barrel structure composed of eight circularly arranged antiparallel strands that are highly conserved among lipocalin molecules such as neutrophil gelatinase-associated lipocalin (NGAL) (WO 2003/029462); and
the concave region formed by the parallel-sheet structure inside the horseshoe-shaped structure constituted by stacked repeats of the leucine-rich-repeat (LRR) module of the variable lymphocyte receptor (VLR) which does not have the immunoglobulin structure and is used in the system of acquired immunity in jawless vertebrate such as lampery and hagfish (WO 2008/016854). Preferred antigen-binding domains of the present invention include, for example, those having antibody heavy-chain and light-chain variable regions. Preferred examples of antigen-binding domains include “single chain Fv (scFv)”, “single chain antibody”, “Fv”, “single chain Fv 2 (scFv2)”, “Fab”, and “F(ab′)₂”.

[0172] “Ion concentration” as used herein includes, for example, metal ion concentration. “Metal ions” refer to ions of group I elements except hydrogen such as alkaline metals and copper group elements, group II elements such as alkaline earth metals and zinc group elements, group III elements except boron, group IV elements except carbon and silicon, group VIII elements such as iron group and platinum group elements, elements belonging to subgroup A of groups V, VI, and VII, and metal elements such as antimony, bismuth, and polonium. Metal atoms have the property of releasing valence electrons to become cations. This is referred to as ionization tendency. Metals with strong ionization tendency are deemed to be chemically active. In the present invention, preferred metal ions include, for example, calcium ion.

[0173] Calcium ion is involved in modulation of many biological phenomena, including contraction of muscles such as skeletal, smooth, and cardiac muscles; activation of movement, phagocytosis, and the like of leukocytes; activation of shape change, secretion, and the like of platelets; activation of lymphocytes; activation of mast cells including secretion of histamine; cell responses mediated by catecholamine a receptor or acetylcholine receptor; exocytosis; release of transmitter substances from neuron terminals; and axoplasmic flow in neurons. Known intracellular calcium ion receptors include troponin C, calmodulin, parvalbumin, and myosin light chain, which have several calcium ion-binding sites and are believed to be derived from a common origin in terms of molecular evolution. There are also many known calcium-binding motifs. Such well-known motifs include, for example, cadherin domains, EF-hand of calmodulin, C2 domain of Protein kinase C, Gla domain of blood coagulation protein Factor IX, C-type lectins of acyaroglycoprotein receptor and mannose-binding receptor, A domains of LDL receptors, annexin, thrombospondin type 3 domain, and EGF-like domains.

[0174] In the present invention, when the metal ion is calcium ion, the conditions of calcium ion concentration include low calcium ion concentration conditions and high calcium ion concentration conditions. “The binding activity varies depending on calcium ion concentration conditions” means that the antigen-binding activity of an antigen-binding molecule varies due to the difference in the conditions between low and high calcium ion concentrations. For example, the antigen-binding activity of an antigen-binding molecule may be higher under a high calcium ion concentration condition than under a low calcium ion concentration condition. Alternatively, the antigen-binding activity of an antigen-binding molecule may be higher under a low calcium ion concentration condition than under a high calcium ion concentration condition.

[0175] Herein, the high calcium ion concentration is not particularly limited to a specific value; however, the concentration may preferably be selected between 100 μM and 10 mM. In another embodiment, the concentration may be selected between 200 μM and 5 mM. In an alternative embodiment, the concentration may be selected between 400 μM and 3 mM. In still another embodiment, the concentration may be selected between 200 μM and 2 mM. Furthermore, the concentration may be selected between 400 μM and 1 mM. In particular, a concentration selected between 500 μM and 2.5 mM, which is close to the plasma (blood) concentration of calcium ion in vivo, is preferred.

[0176] Herein, the low calcium ion concentration is not particularly limited to a specific value;

[0177] however, the concentration may preferably be selected between 0.1 μM and 30 μM. In another embodiment, the concentration may be selected between 0.2 μM and 20 μM. In still another embodiment, the concentration may be selected between 0.5 μM and 10 μM. In an alternative embodiment, the concentration may be selected between 1 μM and 5 μM. Furthermore, the concentration may be selected between 2 μM and 4 μM. In particular, a concentration selected between 1 μM and 5 μM, which is close to the concentration of ionized calcium in early endosomes in vivo, is preferred.

[0178] In the present invention, “the antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition” means that the antigen-binding activity of an antigen-binding molecule is weaker at a calcium ion concentration selected between 0.1 μM and 30 μM than at a calcium ion concentration selected between 100 μM and 10 mM. Preferably, it means that the antigen-binding activity of an antigen-binding molecule is weaker at a calcium ion concentration selected between 0.5 μM and 10 μM than at a calcium ion concentration selected between 200 μM and 5 mM. It particularly preferably means that the antigen-binding activity at the calcium ion concentration in the early endosome in vivo is weaker than that at the in vivo plasma calcium ion concentration; and specifically, it means that the antigen-binding activity of an antigen-binding molecule is weaker at a calcium ion concentration selected between 1 μM and 5 μM than at a calcium ion concentration selected between 500 μM and 2.5 mM.

[0179] Whether the antigen-binding activity of an antigen-binding molecule is changed depending on metal ion concentrations can be determined, for example, by the use of known measurement methods such as those described in the section “Binding Activity” above. For example, in order to confirm that the antigen-binding activity of an antigen-binding molecule becomes higher under a high calcium ion concentration condition than under a low calcium ion concentration condition, the antigen-binding activity of the antigen-binding molecule under low and high calcium ion concentration conditions is compared.

[0180] In the present invention, the expression “the antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition” can also be expressed as “the antigen-binding activity of an antigen-binding molecule is higher under a high calcium ion concentration condition than under a low calcium ion concentration condition”. In the present invention, “the antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition” is sometimes written as “the antigen-binding ability is weaker under a low calcium ion concentration condition than under a high calcium ion concentration condition”. Also, “the antigen-binding activity at a low calcium ion concentration condition is reduced to be lower than that at a high calcium ion concentration condition” may be written as “the antigen-binding ability under a low calcium ion concentration condition is made weaker than that under a high calcium ion concentration condition”.

[0181] When determining the antigen-binding activity, the conditions other than calcium ion concentration can be appropriately selected by those skilled in the art, and are not particularly limited. For example, the activity can be determined at 37° C. in HEPES buffer. For example, Biacore (GE Healthcare) or such can be used for the determination. When the antigen is a soluble antigen, the antigen-binding activity of an antigen-binding molecule can be assessed by flowing the antigen as an analyte over a chip onto which the antigen-binding molecule is immobilized. When the antigen is a membrane antigen, the binding activity of an antigen-binding molecule to the membrane antigen can be assessed by flowing the antigen-binding molecule as an analyte over a chip onto which the antigen is immobilized.

[0182] As long as the antigen-binding activity of an antigen-binding molecule of the present invention at a low calcium ion concentration condition is weaker than that at a high calcium ion concentration condition, the ratio of the antigen-binding activity between that under a low calcium ion concentration condition and under a high calcium ion concentration condition is not particularly limited; and the value of KD (Ca 3 μM)/KD (Ca 2 mM), which is the ratio of the dissociation constant (KD) for an antigen at a low calcium ion concentration condition to the KD at a high calcium ion concentration condition, is preferably 2 or more; more preferably the value of KD (Ca 3 μM)/KD (Ca 2 mM) is 10 or more; and still more preferably the value of KD (Ca 3 μM)/KD (Ca 2 mM) is 40 or more. The upper limit of KD (Ca 3 μM)/KD (Ca 2 mM) value is not particularly limited, and may be any value such as 400, 1000, or 10000, as long as the molecule can be produced by the techniques of those skilled in the art.

[0183] When the antigen is a soluble antigen, KD (dissociation constant) can be used to represent the antigen-binding activity. Meanwhile, when the antigen is a membrane antigen, apparent KD (apparent dissociation constant) can be used to represent the activity. KD (dissociation constant) and apparent KD (apparent dissociation constant) can be determined by methods known to those skilled in the art, for example, using Biacore (GE healthcare), Scatchard plot, or flow cytometer.

[0184] Alternatively, for example, the dissociation rate constant (kd) can also be preferably used as an index to represent the ratio of the antigen-binding activity of an antigen-binding molecule of the present invention between low and high calcium concentration conditions. When the dissociation rate constant (kd) is used instead of the dissociation constant (KD) as an index to represent the binding activity ratio, the ratio of the dissociation rate constant (kd) between low and high calcium concentration conditions, i.e., the value of kd (low calcium concentration condition)/kd (high calcium concentration condition), is preferably 2 or more, more preferably 5 or more, still more preferably 10 or more, and yet more preferably 30 or more. The upper limit of the Kd (low calcium concentration condition)/kd (high calcium concentration condition) value is not particularly limited, and can be any value such as 50, 100, or 200 as long as the molecule can be produced by techniques known to those skilled in the art.

[0185] When the antigen is a soluble antigen, kd (dissociation rate constant) can be used to represent the antigen-binding activity. Meanwhile, when the antigen is a membrane antigen, apparent kd (apparent dissociation rate constant) can be used to represent the antigen-binding activity. The kd (dissociation rate constant) and apparent kd (apparent dissociation rate constant) can be determined by methods known to those skilled in the art, for example, using Biacore (GE healthcare) or flow cytometer. In the present invention, when the antigen-binding activity of an antigen-binding molecule is determined at different calcium ion concentrations, it is preferable to use the same conditions except for the calcium concentrations.

[0186] For example, an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition, which is one embodiment of the present invention, can be obtained via screening of antigen-binding domains or antibodies including the steps of (a) to (c) below:

(a) determining the antigen-binding activity of an antigen-binding domain or antigen-binding molecule at a low calcium concentration condition;
(b) determining the antigen-binding activity of an antigen-binding domain or antigen-binding molecule at a high calcium concentration condition; and
(c) selecting an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is lower at a low calcium concentration condition than at a high calcium concentration condition.

[0187] Moreover, an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition, which is one embodiment of the present invention, can be obtained via screening of antigen-binding domains or antigen-binding molecules, or a library thereof, including the steps of (a) to (c) below:

(a) contacting an antigen with an antigen-binding domain or antigen-binding molecule, or a library thereof at a high calcium concentration condition;
(b) incubating under a low calcium concentration condition an antigen-binding domain or antigen-binding molecule that has bound to the antigen in step (a); and
(c) isolating an antigen-binding domain or antigen-binding molecule dissociated in step (b).

[0188] Furthermore, an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition, which is one embodiment of the present invention, can be obtained via screening of antigen-binding domains or antigen-binding molecules, or a library thereof, including the steps of (a) to (d) below:

(a) contacting an antigen with a library of antigen-binding domains or antigen-binding molecules under a low calcium concentration condition;
(b) selecting an antigen-binding domain or antigen-binding molecule which does not bind to the antigen in step (a);
(c) allowing the antigen-binding domain or antigen-binding molecule selected in step (b) to bind to the antigen under a high calcium concentration condition; and
(d) isolating an antigen-binding domain or antigen-binding molecule that has bound to the antigen in step (c).

[0189] In addition, an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition, which is one embodiment of the present invention, can be obtained by a screening method comprising the steps of (a) to (c) below:

(a) contacting under a high calcium concentration condition a library of antigen-binding domains or antigen-binding molecules with a column onto which an antigen is immobilized;
(b) eluting an antigen-binding domain or antigen-binding molecule that has bound to the column in step (a) from the column under a low calcium concentration condition; and
(c) isolating the antigen-binding domain or antigen-binding molecule eluted in step (b).

[0190] Furthermore, an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition, which is one embodiment of the present invention, can be obtained by a screening method comprising the steps of (a) to (d) below:

(a) allowing under a low calcium concentration condition a library of antigen-binding domains or antigen-binding molecules to pass through a column onto which an antigen is immobilized;
(b) collecting an antigen-binding domain or antigen-binding molecule that has been eluted without binding to the column in step (a);
(c) allowing the antigen-binding domain or antigen-binding molecule collected in step (b) to bind to the antigen under a high calcium concentration condition; and
(d) isolating an antigen-binding domain or antigen-binding molecule that has bound to the antigen in step (c).

[0191] Moreover, an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition, which is one embodiment of the present invention, can be obtained by a screening method comprising the steps of (a) to (d) below:

(a) contacting an antigen with a library of antigen-binding domains or antigen-binding molecules under a high calcium concentration condition;
(b) obtaining an antigen-binding domain or antigen-binding molecule that has bound to the antigen in step (a);
(c) incubating under a low calcium concentration condition the antigen-binding domain or antigen-binding molecule obtained in step (b); and
(d) isolating an antigen-binding domain or antigen-binding molecule whose antigen-binding activity in step (c) is weaker than the criterion for the selection of step (b).

[0192] The above-described steps may be repeated twice or more times. Thus, the present invention provides antigen-binding domains or antigen-binding molecules whose antigen-binding activity is lower at a low calcium ion concentration condition than at a high calcium ion concentration condition, which are obtained by screening methods that further comprises the step of repeating twice or more times steps (a) to (c) or (a) to (d) in the above-described screening methods. The number of cycles of steps (a) to (c) or (a) to (d) is not particularly limited, but generally is 10 or less.

[0193] In the above-mentioned screening methods, the antigen-binding activity of an antigen-binding domain or antigen-binding molecule under a low calcium concentration condition is not particularly limited as long as it is antigen-binding activity at an ionized calcium concentration of between 0.1 μM and 30 μM, but preferably is antigen-binding activity at an ionized calcium concentration of between 0.5 μM and 10 μM. More preferably, it is antigen-binding activity at the ionized calcium concentration in the early endosome in vivo, specifically, between 1 μM and 5 μM. Meanwhile, the antigen-binding activity of an antigen-binding domain or antigen-binding molecule under a high calcium concentration condition is not particularly limited, as long as it is antigen-binding activity at an ionized calcium concentration of between 100 μM and 10 mM, but preferably is antigen-binding activity at an ionized calcium concentration of between 200 μM and 5 mM. More preferably, it is antigen-binding activity at the ionized calcium concentration in plasma in vivo, specifically, between 0.5 mM and 2.5 mM.

[0194] The method described in WO 2012/073992 (for example, paragraphs 0200-0213) and such may be exemplified as a method of screening for an antigen-binding molecule or an antigen-binding domain having lower antigen-binding activity under low calcium ion concentration conditions than under high calcium ion concentration conditions, which is an embodiment provided by the present invention.

[0195] The antigen-binding activity of an antigen-binding domain or antigen-binding molecule can be measured by methods known to those skilled in the art. Conditions other than the ionized calcium concentration can be determined by those skilled in the art. The antigen-binding activity of an antigen-binding domain or antigen-binding molecule can be evaluated as a dissociation constant (KD), apparent dissociation constant (apparent KD), dissociation rate constant (kd), apparent dissociation constant (apparent kd), and such. These can be determined by methods known to those skilled in the art, for example, using Biacore (GE healthcare), Scatchard plot, or FACS.

[0196] In the present invention, the step of selecting an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is higher under a high calcium concentration condition than under a low calcium concentration condition is synonymous with the step of selecting an antigen-binding domain or antigen-binding molecule whose antigen-binding activity is lower under a low calcium concentration condition than under a high calcium concentration condition.

[0197] As long as the antigen-binding activity is higher under a high calcium concentration condition than under a low calcium concentration condition, the difference in the antigen-binding activity between high and low calcium concentration conditions is not particularly limited; however, the antigen-binding activity under a high calcium concentration condition is preferably twice or more, more preferably 10 times or more, and still more preferably 40 times or more than that under a low calcium concentration condition.

[0198] Antigen-binding domains or antigen-binding molecules of the present invention to be screened by the screening methods described above may be any antigen-binding domains and antigen-binding molecules. For example, it is possible to screen the above-described antigen-binding domains or antigen-binding molecules. For example, antigen-binding domains or antigen-binding molecules having natural sequences or substituted amino acid sequences may be screened.

[0199] For example, in an embodiment provided by the present invention, the method described in WO 2012/073992 (for example, paragraphs 0200-0213) and such may be exemplified as a method of screening for an antigen-binding molecule or an antigen-binding domain having lower antigen-binding activity under a low calcium ion concentration condition than under a high calcium ion concentration condition.

[0200] Antigen-binding domains or antigen-binding molecules of the present invention whose antigen-binding activities change according to the calcium ion concentration condition, which are screened by the above-described screening methods, may be prepared in any manner. For example, when the metal ion concentration refers to calcium ion concentration, it is possible to use preexisting antigen-binding domains or antigen-binding molecules, preexisting libraries (phage library, etc.), antibodies or libraries prepared from hybridomas obtained by immunizing animals or from B cells of immunized animals, antibodies or libraries obtained by introducing amino acids capable of chelating calcium (for example, aspartic acid and glutamic acid) or introducing non-natural amino acid mutations into the above-described antibodies or libraries (libraries produced by increasing the content of amino acids capable of chelating calcium (such as aspartic acid and glutamic acid) or non-natural amino acids, libraries prepared by introducing amino acids capable of chelating calcium (such as aspartic acid and glutamic acid) or non-natural amino acid mutations at particular positions, or the like).

[0201] Examples of the amino acids that alter the antigen-binding activity of antigen-binding molecules according to the ion concentration condition as described above may be any types of amino acids as long as the amino acids form a calcium-binding motif. Calcium-binding motifs are well known to those skilled in the art and have been described in details (for example, Springer et al. (Cell (2000) 102, 275-277); Kawasaki and Kretsinger (Protein Prof (1995) 2, 305-490); Moncrief et al. (J. Mol. Evol. (1990) 30, 522-562); Chauvaux et al. (Biochem. J. (1990) 265, 261-265); Bairoch and Cox (FEBS Lett. (1990) 269, 454-456); Davis (New Biol. (1990) 2, 410-419); Schaefer et al. (Genomics (1995) 25, 638-643); Economou et al. (EMBO J. (1990) 9, 349-354); Wurzburg et al. (Structure. (2006) 14, 6, 1049-1058)). Specifically, any known calcium-binding motifs, including type C lectins such as ASGPR, CD23, MBR, and DC-SIGN, can be included in antigen-binding molecules of the present invention. Preferred examples of such preferred calcium-binding motifs also include, in addition to those described above, for example, the calcium-binding motif in the antigen-binding domain of SEQ ID NO: 45.

[0202] Furthermore, as amino acids that alter the antigen-binding activity of antigen-binding domains included in the antigen-binding molecules of the present invention depending on calcium ion concentration conditions, for example, amino acids having metal-chelating activity may also be preferably used. Examples of such metal-chelating amino acids include, for example, serine (Ser (S)), threonine (Thr (T)), asparagine (Asn (N)), glutamine (Gln (Q)), aspartic acid (Asp (D)), and glutamic acid (Glu (E)).

[0203] Positions in the antigen-binding domains at which the above-described amino acids are contained are not particularly limited to particular positions, and may be any positions within the heavy chain variable region or light chain variable region that forms an antigen-binding domain, as long as they alter the antigen-binding activity of antigen-binding molecules depending on calcium ion concentration conditions. In a non-limiting embodiment, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose heavy chain antigen-binding domains contain amino acids that alter the antigen-binding activity of the antigen-binding molecules depending on calcium ion concentration conditions. In another non-limiting embodiment, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose heavy chain CDR3 domains contain the above-mentioned amino acids. In still another embodiment, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose heavy chain CDR3 domains contain the above-mentioned amino acids at positions 95, 96, 100a, and/or 101 as indicated according to the Kabat numbering system.

[0204] Meanwhile, in a non-limiting embodiment of the present invention, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose light chain antigen-binding domains contain amino acids that alter the antigen-binding activity of antigen-binding molecules depending on calcium ion concentration conditions. In another non-limiting embodiment, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose light chain CDR1 domains contain the above-mentioned amino acids. In still another embodiment, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose light chain CDR1 domains contain the above-mentioned amino acids at positions 30, 31, and/or 32 as indicated according to the Kabat numbering system.

[0205] In another non-limiting embodiment, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose light chain CDR2 domains contain the above-mentioned amino acid residues. In yet another non-limiting embodiment, the present invention provides libraries mainly composed of antigen-binding molecules whose sequences are different from one another and whose light chain CDR2 domains contain the above-mentioned amino acid residues at position 50 as indicated according to the Kabat numbering system.

[0206] In still another embodiment of the present invention, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose light chain CDR3 domains contain the above-mentioned amino acid residues. In an alternative embodiment, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose light chain CDR3 domains contain the above-mentioned amino acid residues at position 92 as indicated according to the Kabat numbering system.

[0207] Furthermore, in a different embodiment of the present invention, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and in which two or three CDRs selected from the above-described light chain CDR1, CDR2, and CDR3 contain the aforementioned amino acid residues. Moreover, antigen-binding domains of the present invention can be obtained from a library mainly composed of antigen-binding molecules whose sequences are different from one another and whose light chains contain the aforementioned amino acid residues at any one or more of positions 30, 31, 32, 50, and/or 92 as indicated according to the Kabat numbering system.

[0208] In a particularly preferred embodiment, the framework sequences of the light chain and/or heavy chain variable region of an antigen-binding molecule preferably contain human germ line framework sequences. Thus, in an embodiment of the present invention, when the framework sequences are completely human sequences, it is expected that when such an antigen-binding molecule of the present invention is administered to humans (for example, to treat diseases), it induces little or no immunogenic response. In the above sense, the phrase “containing a germ line sequence” in the present invention means that a part of the framework sequences in the present invention is identical to a part of any human germ line framework sequences. For example, when the heavy chain FR2 sequence of an antigen-binding molecule in the present invention is a combination of heavy chain FR2 sequences of different human germ line framework sequences, such a molecule is also an antigen-binding molecule in the present invention “containing a germ line sequence”.

[0209] Preferred examples of the frameworks include, for example, fully human framework region sequences currently known, which are included in the website of V-Base (http://vbase.mrc-cpe.cam.ac.uk/) or others. Those framework region sequences can be appropriately used as a germ line sequence contained in an antigen-binding molecule of the present invention. The germ line sequences may be categorized according to their similarity (Tomlinson et al. (J. Mol. Biol. (1992) 227, 776-798); Williams and Winter (Eur. J. Immunol. (1993) 23, 1456-1461); Cox et al. (Nat. Genetics (1994) 7, 162-168)). Appropriate germ line sequences can be selected from VK, which is grouped into seven subgroups; V2, which is grouped into ten subgroups; and VH, which is grouped into seven subgroups.

[0210] Fully human VH sequences preferably include, but are not limited to, for example, VH sequences of:

subgroup VH1 (for example, VH1-2, VH1-3, VH1-8, VH1-18, VH1-24, VH1-45, VH1-46, VH1-58, and VH1-69);
subgroup VH2 (for example, VH2-5, VH2-26, and VH2-70);
subgroup VH3 (VH3-7, VH3-9, VH3-11, VH3-13, VH3-15, VH3-16, VH3-20, VH3-21, VH3-23, VH3-30, VH3-33, VH3-35, VH3-38, VH3-43, VH3-48, VH3-49, VH3-53, VH3-64, VH3-66, VH3-72, VH3-73, and VH3-74);
subgroup VH4 (VH4-4, VH4-28, VH4-31, VH4-34, VH4-39, VH4-59, and VH4-61);
subgroup VH5 (VH5-51);
subgroup VH6 (VH6-1); and
subgroup VH7 (VH7-4 and VH7-81).
These are also described in known documents (Matsuda et al. (J. Exp. Med. (1998) 188, 1973-1975)) and such, and thus persons skilled in the art can appropriately design antigen-binding molecules of the present invention based on the information of these sequences. It is also preferable to use other fully human frameworks or framework sub-regions.

[0211] Fully human Vκ sequences preferably include, but are not limited to, for example:

A20, A30, L1, L4, L5, L8, L9, L11, L12, L14, L15, L18, L19, L22, L23, L24, O2, O4, O8, O12, O14, and O18 grouped into subgroup Vk1;
A1, A2, A3, A5, A7, A17, A18, A19, A23, O1, and O11, grouped into subgroup Vk2;
A11, A27, L2, L6, L10, L16, L20, and L25, grouped into subgroup Vk3;
B3, grouped into subgroup Vk4;
B2 (herein also referred to as Vk5-2), grouped into subgroup Vk5; and
A10, A14, and A26, grouped into subgroup Vk6
(Kawasaki et al. (Eur. J. Immunol. (2001) 31, 1017-1028); Schable and Zachau (Biol. Chem. Hoppe Seyler (1993) 374, 1001-1022); Brensing-Kuppers et al. (Gene (1997) 191, 173-181)).

[0212] Fully human Vλ sequences preferably include, but are not limited to, for example:

V1-2, V1-3, V1-4, V1-5, V1-7, V1-9, V1-11, V1-13, V1-16, V1-17, V1-18, V1-19, V1-20, and V1-22, grouped into subgroup VL1;
V2-1, V2-6, V2-7, V2-8, V2-11, V2-13, V2-14, V2-15, V2-17, and V2-19, grouped into subgroup VL1;
V3-2, V3-3, and V3-4, grouped into subgroup VL3;
V4-1, V4-2, V4-3, V4-4, and V4-6, grouped into subgroup VL4; and
V5-1, V5-2, V5-4, and V5-6, grouped into subgroup VL5 (Kawasaki et al. (Genome Res. (1997) 7, 250-261)).

[0213] Normally, these framework sequences are different from one another at one or more amino acid residues. These framework sequences can be used in combination with “at least one amino acid residue that alters the antigen-binding activity of an antigen-binding molecule depending on ion concentration conditions” in the present invention. Other examples of the fully human frameworks used in combination with “at least one amino acid residue that alters the antigen-binding activity of an antigen-binding molecule depending on ion concentration conditions” in the present invention include, but are not limited to, for example, KOL, NEWM, REI, EU, TUR, TEI, LAY, and POM (for example, Kabat et al. (1991) supra; Wu et al. (J. Exp. Med. (1970) 132, 211-250)).

[0214] Without being bound by a particular theory, one reason for the expectation that the use of germ line sequences precludes adverse immune responses in most individuals is believed to be as follows. As a result of the process of affinity maturation during normal immune responses, somatic mutation occurs frequently in the variable regions of immunoglobulin. Such mutations mostly occur around CDRs whose sequences are hypervariable, but also affect residues of framework regions. Such framework mutations do not exist on the germ line genes, and also they are less likely to be immunogenic in patients. On the other hand, the normal human population is exposed to most of the framework sequences expressed from the germ line genes. As a result of immunotolerance, these germ line frameworks are expected to have low or no immunogenicity in patients. To maximize the possibility of immunotolerance, variable region-encoding genes may be selected from a group of commonly occurring functional germ line genes.

[0215] Known methods such as site-directed mutagenesis (Kunkel et al. (Proc. Natl. Acad. Sci. USA (1985) 82, 488-492)) and overlap extension PCR can be appropriately employed to produce the antigen-binding molecules of the present invention in which the above-described variable region sequences, heavy or light chain variable region sequences, CDR sequences, or framework sequences contain amino acids that alter the antigen-binding activity of the antigen-binding molecules depending on calcium ion concentration conditions.

[0216] For example, a library which contains a plurality of antigen-binding molecules of the present invention whose sequences are different from one another can be constructed by combining heavy chain variable regions prepared as a randomized variable region sequence library with a light chain variable region selected as a framework sequence originally containing at least one amino acid residue that alters the antigen-binding activity of the antigen-binding molecule depending on calcium ion concentration conditions. As a non-limiting example, when the ion concentration is calcium ion concentration, such preferred libraries include, for example, those constructed by combining the light chain variable region sequence of SEQ ID NO: 45 (Vk5-2) and the heavy chain variable region produced as a randomized variable region sequence library.

[0217] Alternatively, a light chain variable region sequence selected as a framework region originally containing at least one amino acid residue that alters the antigen-binding activity of an antigen-binding domain or an antigen-binding molecule according to the calcium ion concentration condition as mentioned above can be designed to contain various amino acid residues other than the above amino acid residues. In the present invention, such residues are referred to as flexible residues. The number and position of flexible residues are not limited to particular embodiments as long as the antigen-binding activity of the antigen-binding domain or antigen-binding molecule of the present invention varies depending on the ion concentration condition. Specifically, the CDR sequences and/or FR sequences of the heavy chain and/or light chain may contain one or more flexible residues. For example, when the ion concentration is calcium ion concentration, non-limiting examples of flexible residues to be introduced into the light chain variable region sequence of SEQ ID NO: 45 (Vk5-2) include the amino acid residues listed in Tables 1 or 2.

TABLE 1
	Kabat
	NUM-
CDR	BERING	70% OF AMINO ACID OF THE TOTAL
CDR1	28	S: 100%
	29	I: 100%
	30	E: 72%	N: 14%	S: 14%
	31	D: 100%
	32	D: 100%
	33	L: 100%
	34	A: 70%	N: 30%
CDR2	50	E: 100%
	51	A: 100%
	52	S: 100%
	53	H: 5%	N: 25%	S: 45%	T: 25%
	54	L: 100%
	55	Q: 100%
	56	S: 100%
CDR3	90	Q: 100%
	91	H: 25%	S: 15%	R: 15%	Y: 45%
	92	D: 80%	N: 10%	S: 10%
	93	D: 5%	G: 10%	N: 25%	S: 50%	R: 10%
	94	S: 50%	Y: 50%
	95	P: 100%
	96	L: 50%	Y: 50%
(Position indicates Kabat numbering.)

TABLE 2
	Kabat
	NUM-
CDR	BERING	30% OF AMINO ACID OF THE TOTAL
CDR1	28	S: 100%
	29	I: 100%
	30	E: 83%	S: 17%
	31	D: 100%
	32	D: 100%
	33	L: 100%
	34	A: 70%	N: 30%
CDR2	50	H: 100%
	51	A: 100%
	52	S: 100%
	53	H: 5%	N: 25%	S: 45%	T: 25%
	54	L: 100%
	55	Q: 100%
	56	S: 100%
CDR3	90	Q: 100%
	91	H: 25%	S: 15%	R: 15%	Y: 45%
	92	D: 80%	N: 10%	S: 10%
	93	D: 5%	G: 10%	N: 25%	S: 50%	R: 10%
	94	S: 50%	Y: 50%
	95	P: 100%
	96	L: 50%	Y: 50%
(Position indicates Kabat numbering.)

[0218] Herein, flexible residues refer to amino acid residue variations present at hypervariable positions at which several different amino acids are present on the light chain and heavy chain variable regions when the amino acid sequences of known and/or native antibodies or antigen-binding domains are compared. Hypervariable positions are generally located in the CDR. In an embodiment, the data provided by Kabat, Sequences of Proteins of Immunological Interest (National Institute of Health Bethesda Md.) (1987 and 1991) is useful to determine hypervariable positions in known and/or native antibodies. Furthermore, several databases on the Internet (http://vbase.mrc-cpe.cam.ac.uk/, http://www.bioinf.org.uk/abs/index.html) provide the collected sequences of many human light chains and heavy chains and their locations. The information on the sequences and locations is useful to determine hypervariable positions in the present invention. According to the present invention, when a certain amino acid position has preferably about 2 to about 20 possible amino acid residue variations, preferably about 3 to about 19, preferably about 4 to about 18, preferably 5 to 17, preferably 6 to 16, preferably 7 to 15, preferably 8 to 14, preferably 9 to 13, and preferably 10 to 12 possible different amino acid residue variations, the position is hypervariable. In some embodiments, a certain amino acid position may have preferably at least about 2, preferably at least about 4, preferably at least about 6, preferably at least about 8, preferably about 10, and preferably about 12 possible different amino acid residue variations.

[0219] Alternatively, a library containing a plurality of antigen-binding molecules of the present invention whose sequences are different from one another can be constructed by combining heavy chain variable regions produced as a randomized variable region sequence library with light chain variable regions into which at least one amino acid residue that alters the antigen-binding activity of antigen-binding molecules depending on ion concentration conditions as mentioned above is introduced. When the ion concentration is calcium ion concentration, non-limiting examples of such libraries preferably include, for example, libraries in which heavy chain variable regions produced as a randomized variable region sequence library are combined with light chain variable region sequences in which a particular residue(s) in a germ line sequence such as SEQ ID NO: 46 (Vk1), SEQ ID NO: 47 (Vk2), SEQ ID NO: 48 (Vk3), or SEQ ID NO: 49 (Vk4) has been substituted with at least one amino acid residue that alters the antigen-binding activity of an antigen-binding molecule depending on calcium ion concentration conditions. Non-limiting examples of such amino acid residues include amino acid residues in light chain CDR1. Furthermore, non-limiting examples of such amino acid residues include amino acid residues in light chain CDR2. In addition, non-limiting, other examples of such amino acid residues also include amino acid residues in light chain CDR3.

[0220] Non-limiting examples of such amino acid residues contained in light chain CDR1 include those at positions 30, 31, and/or 32 in the CDR1 of light chain variable region as indicated by EU numbering. Furthermore, non-limiting examples of such amino acid residues contained in light chain CDR2 include an amino acid residue at position 50 in the CDR2 of light chain variable region as indicated by Kabat numbering. Moreover, non-limiting examples of such amino acid residues contained in light chain CDR3 include an amino acid residue at position 92 in the CDR3 of light chain variable region as indicated by Kabat numbering. These amino acid residues can be contained alone or in combination as long as they form a calcium-binding motif and/or as long as the antigen-binding activity of an antigen-binding molecule varies depending on calcium ion concentration conditions. Meanwhile, as troponin C, calmodulin, parvalbumin, and myosin light chain, which have several calcium ion-binding sites and are believed to be derived from a common origin in terms of molecular evolution, are known, the light chain CDR1, CDR2, and/or CDR3 can be designed to have their binding motifs. For example, it is possible to use cadherin domains, EF hand of calmodulin, C2 domain of Protein kinase C, Gla domain of blood coagulation protein FactorIX, C type lectins of acyaroglycoprotein receptor and mannose-binding receptor, A domains of LDL receptors, annexin, thrombospondin type 3 domain, and EGF-like domains in an appropriate manner for the above purposes.

[0221] When heavy chain variable regions produced as a randomized variable region sequence library and light chain variable regions into which at least one amino acid residue that alters the antigen-binding activity of an antigen-binding molecule depending on ion concentrations has been introduced are combined as described above, the sequences of the light chain variable regions can be designed to contain flexible residues in the same manner as described above. The number and position of such flexible residues are not limited to particular embodiments as long as the antigen-binding activity of antigen-binding molecules of the present invention varies depending on ion concentration conditions. Specifically, the CDR sequences and/or FR sequences of heavy chain and/or light chain can contain one or more flexible residues. For example, when the ion concentration is calcium ion concentration, non-limiting examples of flexible residues to be introduced into the sequence of light chain variable region include the amino acid residues listed in Tables 1 and 2.

[0222] The preferred heavy chain variable regions to be combined include, for example, randomized variable region libraries. Known methods are combined as appropriate to produce a randomized variable region library. In a non-limiting embodiment of the present invention, an immune library constructed based on antibody genes derived from lymphocytes of animals immunized with a specific antigen, patients with infections, persons with an elevated antibody titer in blood as a result of vaccination, cancer patients, or auto immune disease patients, may be preferably used as a randomized variable region library.

[0223] In another non-limiting embodiment of the present invention, a synthetic library produced by replacing the CDR sequences of V genes in genomic DNA or functional reshaped V genes with a set of synthetic oligonucleotides containing sequences encoding codon sets of an appropriate length can also be preferably used as a randomized variable region library. In this case, since sequence diversity is observed in the heavy chain CDR3 sequence, it is also possible to replace the CDR3 sequence only. A criterion of giving rise to diversity in amino acids in the variable region of an antigen-binding molecule is that diversity is given to amino acid residues at surface-exposed positions in the antigen-binding molecule. The surface-exposed position refers to a position that is considered to be able to be exposed on the surface and/or contacted with an antigen, based on structure, ensemble of structures, and/or modeled structure of an antigen-binding molecule. In general, such positions are CDRs. Preferably, surface-exposed positions are determined using coordinates from a three-dimensional model of an antigen-binding molecule using a computer program such as the InsightII program (Accelrys). Surface-exposed positions can be determined using algorithms known in the art (for example, Lee and Richards (J. Mol. Biol. (1971) 55, 379-400); Connolly (J. Appl. Cryst. (1983) 16, 548-558)). Determination of surface-exposed positions can be performed using software suitable for protein modeling and three-dimensional structural information obtained from an antibody. Software that can be used for these purposes preferably includes SYBYL Biopolymer Module software (Tripos Associates). Generally or preferably, when an algorithm requires a user input size parameter, the “size” of a probe which is used in the calculation is set at about 1.4 Angstrom or smaller in radius. Furthermore, methods for determining surface-exposed regions and areas using software for personal computers are described by Pacios (Comput. Chem. (1994) 18 (4), 377-386; J. Mol. Model. (1995) 1, 46-53).

[0224] In another non-limiting embodiment of the present invention, a naive library, which is constructed from antibody genes derived from lymphocytes of healthy persons and whose repertoire comprises naive sequences, which are antibody sequences with no bias, can also be particularly preferably used as a randomized variable region library (Gejima et al. (Human Antibodies (2002) 11, 121-129); Cardoso et al. (Scand. J. Immunol. (2000) 51, 337-344)). Herein, an amino acid sequence comprising a naive sequence refers to an amino acid sequence obtained from such a naive library.

[0225] In one embodiment of the present invention, an antigen-binding domain of the present invention can be obtained from a library containing a plurality of antigen-binding molecules of the present invention whose sequences are different from one another, prepared by combining light chain variable regions constructed as a randomized variable region sequence library with a heavy chain variable region selected as a framework sequence that originally contains “at least one amino acid residue that alters the antigen-binding activity of an antigen-binding molecule depending on ion concentration conditions”. When the ion concentration is calcium ion concentration, non-limiting examples of such libraries preferably include those constructed by combining light chain variable regions constructed as a randomized variable region sequence library with the sequence of heavy chain variable region of SEQ ID NO: 50 (6RL#9-IgG1) or SEQ ID NO: 51 (6KC4-1#85-IgG1). Alternatively, such a library can be constructed by selecting appropriate light chain variable regions from those having germ line sequences, instead of light chain variable regions constructed as a randomized variable region sequence library. Such preferred libraries include, for example, those in which the sequence of heavy chain variable region of SEQ ID NO: 50 (6RL#9-IgG1) or SEQ ID NO: 51 (6KC4-1#85-IgG1) is combined with light chain variable regions having germ line sequences.

[0226] Alternatively, the sequence of a heavy chain variable region selected as a framework sequence that originally contains “at least one amino acid residue that alters the antigen-binding activity of an antigen-binding molecule depending on ion concentration condition” as mentioned above can be designed to contain flexible residues. The number and position of the flexible residues are not particularly limited as long as the antigen-binding activity of an antigen-binding molecule of the present invention varies depending on ion concentration conditions. Specifically, the CDR and/or FR sequences of heavy chain and/or light chain can contain one or more flexible residues. When the ion concentration is calcium ion concentration, non-limiting examples of flexible residues to be introduced into the sequence of heavy chain variable region of SEQ ID NO: 50 (6RL#9-IgG1) include all amino acid residues of heavy chain CDR1 and CDR2 and the amino acid residues of the heavy chain CDR3 except those at positions 95, 96, and/or 100a. Alternatively, non-limiting examples of flexible residues to be introduced into the sequence of heavy chain variable region of SEQ ID NO: 51 (6KC4-1#85-IgG1) include all amino acid residues of heavy chain CDR1 and CDR2 and the amino acid residues of the heavy chain CDR3 except those at amino acid positions 95 and/or 101.

[0227] Alternatively, a library containing a plurality of antigen-binding molecules whose sequences are different from one another can be constructed by combining light chain variable regions constructed as a randomized variable region sequence library or light chain variable regions having germ line sequences with heavy chain variable regions into which “at least one amino acid residue that alters the antigen-binding activity of an antigen-binding molecule depending on ion concentration condition” has been introduced as mentioned above. When the ion concentration is calcium ion concentration, non-limiting examples of such libraries preferably include those in which light chain variable regions constructed as a randomized variable region sequence library or light chain variable regions having germ line sequences are combined with the sequence of a heavy chain variable region in which a particular residue(s) has been substituted with at least one amino acid residue that alters the antigen-binding activity of an antigen-binding molecule depending on calcium ion concentration conditions. Non-limiting examples of such amino acid residues include amino acid residues of the heavy chain CDR1. Further non-limiting examples of such amino acid residues include amino acid residues of the heavy chain CDR2. In addition, non-limiting examples of such amino acid residues also include amino acid residues of the heavy chain CDR3. Non-limiting examples of such amino acid residues of heavy chain CDR3 include the amino acids of positions 95, 96, 100a, and/or 101 in the CDR3 of heavy chain variable region as indicated by the Kabat numbering. Furthermore, these amino acid residues can be contained alone or in combination as long as they form a calcium-binding motif and/or the antigen-binding activity of an antigen-binding molecule varies depending on calcium ion concentration conditions.

[0228] When light chain variable regions constructed as a randomized variable region sequence library or light chain variable regions having germ line sequence are combined with a heavy chain variable region into which at least one amino acid residue that alter the antigen-binding activity of an antigen-binding molecule depending on ion concentration conditions as mentioned above has been introduced, the sequence of the heavy chain variable region can also be designed to contain flexible residues in the same manner as described above. The number and position of flexible residues are not particularly limited as long as the antigen-binding activity of an antigen-binding molecule of the present invention varies depending on ion concentration conditions. Specifically, the heavy chain CDR and/or FR sequences may contain one or more flexible residues. Furthermore, randomized variable region libraries can be preferably used as amino acid sequences of CDR1, CDR2, and/or CDR3 of the heavy chain variable region other than the amino acid residues that alter the antigen-binding activity of an antigen-binding molecule. When germ line sequences are used as light chain variable regions, non-limiting examples of such sequences include those of SEQ ID NO: 46 (Vk1), SEQ ID NO: 47 (Vk2), SEQ ID NO: 48 (Vk3), and SEQ ID NO: 49 (Vk4).

[0229] Any of the above-described amino acids that alter the antigen-binding activity of an antigen-binding molecule depending on calcium ion concentration conditions can be preferably used, as long as they form a calcium-binding motif. Specifically, such amino acids include electron-donating amino acids. Preferred examples of such electron-donating amino acids include serine, threonine, asparagine, glutamic acid, aspartic acid, and glutamic acid.

[0230] An example of the “ion concentration condition” of the present invention includes a “pH condition”. A pH condition may also be referred to as a hydrogen ion concentration condition. In the present invention, the condition of concentration of proton, i.e., the nucleus of hydrogen atom, is treated as synonymous with a hydrogen index (pH) condition. When the activity of hydrogen ion in an aqueous solution is represented as aH+, pH is defined as −log 10aH+. When the ionic strength of the aqueous solution is low (for example, lower than 10⁻³), aH+ is nearly equal to the hydrogen ion strength. For example, the ionic product of water at 25° C. and 1 atmosphere is Kw=aH+aOH=10⁻¹⁴, and therefore in pure water, aH+=aOH=10⁻⁷. In this case, pH=7 is neutral; an aqueous solution whose pH is lower than 7 is acidic or whose pH is greater than 7 is alkaline.

[0231] In the present invention, when pH condition is used as the ion concentration condition, pH conditions include conditions of high hydrogen ion concentration or low pHs, i.e., an acidic pH range condition, and conditions of low hydrogen ion concentration or high pHs, i.e., a neutral pH range condition. “The antigen-binding activity of an antigen-binding domain contained in the antigen-binding molecule of the present invention varies depending on pH condition” means that the antigen-binding activity of an antigen-binding domain contained in an antigen-binding molecule varies due to the difference in conditions of a high hydrogen ion concentration or low pH (an acidic pH range) and a low hydrogen ion concentration or high pH (a neutral pH range). This includes, for example, the case where the antigen-binding activity of an antigen-binding molecule is higher under a neutral pH range condition than under an acidic pH range condition and the case where the antigen-binding activity of an antigen-binding molecule is higher under an acidic pH range condition than under a neutral pH range condition.

[0232] Herein, neutral pH range is not limited to a specific value and is preferably selected from between pH 6.7 and pH 10.0. In another embodiment, the pH can be selected from between pH 6.7 and pH 9.5. In still another embodiment, the pH can be selected from between pH 7.0 and pH 9.0. In yet another embodiment, the pH can be selected from between pH 7.0 and pH 8.0. In particular, the preferred pH includes pH 7.4, which is close to the pH of plasma (blood) in vivo.

[0233] Herein, an acidic pH range is not limited to a specific value and is preferably selected from between pH 4.0 and pH 6.5. In another embodiment, the pH can be selected from between pH 4.5 and pH 6.5. In still another embodiment, the pH can be selected from between pH 5.0 and pH 6.5. In yet another embodiment, the pH can be selected from between pH 5.5 and pH 6.5. In particular, the preferred pH includes pH 5.8, which is close to the ionized calcium concentration in the early endosome in vivo.

[0234] In the present invention, “the antigen-binding activity under a condition of a high hydrogen ion concentration or low pH (an acidic pH range) is lower than that under a condition of a low hydrogen ion concentration or high pH (a neutral pH range)” means that the antigen-binding activity of antigen-binding domain or antigen-binding molecule comprising the domain of the present invention at a pH selected from between pH 4.0 and pH 6.5 is weaker than that at a pH selected from between pH 6.7 and pH 10.0; preferably means that the antigen-binding activity of an antigen-binding domain or antigen-binding molecule comprising the domain at a pH selected from between pH 4.5 and pH 6.5 is weaker than that at a pH selected from between pH 6.7 and pH 9.5; more preferably, means that the antigen-binding activity of an antigen-binding molecule at a pH selected from between pH 5.0 and pH 6.5 is weaker than that at a pH selected from between pH 7.0 and pH 9.0; still more preferably means that the antigen-binding activity of an antigen-binding molecule at a pH selected from between pH 5.5 and pH 6.5 is weaker than that at a pH selected from between pH 7.0 and pH 8.0; particularly preferably means that the antigen-binding activity at the pH in the early endosome in vivo is weaker than the antigen-binding activity at the pH of plasma in vivo; and specifically means that the antigen-binding activity of an antigen-binding molecule at pH 5.8 is weaker than the antigen-binding activity at pH 7.4.

[0235] Whether the antigen-binding activity of an antigen-binding domain or antigen-binding molecule comprising the domain has changed by the pH condition can be determined, for example, by the use of known measurement methods such as those described in the section “Binding Activity” above. For example, the binding activity is measured under different pH conditions using the measurement methods described above. For example, the antigen-binding activity of an antigen-binding domain or antigen-binding molecule comprising the domain is compared under the conditions of acidic pH range and neutral pH range to confirm that binding activity of the domain or the molecule changes to be higher under the condition of neutral pH range than that under the condition of acidic pH range.

[0236] Furthermore, in the present invention, the expression “the antigen-binding activity under a condition of high hydrogen ion concentration or low pH, i.e., under an acidic pH range condition, is lower than that under a condition of low hydrogen ion concentration or high pH, i.e., under a neutral pH range condition” can also be expressed as “the antigen-binding activity of an antigen-binding domain or antigen-binding molecule comprising the domain under a condition of low hydrogen ion concentration or high pH, i.e., under a neutral pH range condition, is higher than that under a condition of high hydrogen ion concentration or low pH, i.e., under an acidic pH range condition”. In the present invention, “the antigen-binding activity under a condition of high hydrogen ion concentration or low pH, i.e., under an acidic pH range condition, is lower than that under a condition of low hydrogen ion concentration or high pH, i.e., under a neutral pH range condition” may be described as “the antigen-binding activity under a condition of high hydrogen ion concentration or low pH, i.e., under an acidic pH range condition, is weaker than the antigen-binding ability under a condition of low hydrogen ion concentration or high pH, i.e., under a neutral pH range condition”. Alternatively, “the antigen-binding activity under a condition of high hydrogen ion concentration or low pH, i.e., under an acidic pH range condition, is reduced to be lower than that under a condition of low hydrogen ion concentration or high pH, i.e., under a neutral pH range condition” may be described as “the antigen-binding activity under a condition of high hydrogen ion concentration or low pH, i.e., under an acidic pH range condition, is reduced to be weaker than the antigen-binding ability under a condition of low hydrogen ion concentration or high pH, i.e., under a neutral pH range condition”.

[0237] The conditions other than hydrogen ion concentration or pH for measuring the antigen-binding activity may be suitably selected by those skilled in the art and are not particularly limited. Measurements can be carried out, for example, at 37° C. using HEPES buffer. Measurements can be carried out, for example, using Biacore (GE Healthcare). When the antigen is a soluble antigen, the antigen-binding activity of antigen-binding domain or antigen-binding molecule comprising the domain can be determined by assessing the binding activity to the soluble antigen by flowing the antigen as an analyte into a chip immobilized with the antigen-binding domain or the antigen-binding molecule comprising the domain. When the antigen is a membrane antigen, the binding activity to the membrane antigen can be assessed by flowing the antigen-binding domain or the antigen-binding molecule comprising the domain as an analyte into a chip immobilized with the antigen.

[0238] As long as the antigen-binding activity of an antigen-binding molecule of the present invention at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition is weaker than that at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, the ratio of the antigen-binding activity between that under a condition of high hydrogen ion concentration or low pH, i.e., under an acidic pH range condition, and under a condition of low hydrogen ion concentration or high pH, i.e., under a neutral pH range condition is not particularly limited, and the value of KD (pH 5.8)/KD (pH 7.4), which is the ratio of the dissociation constant (KD) for an antigen at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition to the KD at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, is preferably 2 or more; more preferably the value of KD (pH 5.8)/KD (pH 7.4) is 10 or more; and still more preferably the value of KD (pH 5.8)/KD (pH 7.4) is 40 or more. The upper limit of KD (pH 5.8)/KD (pH 7.4) value is not particularly limited, and may be any value such as 400, 1000, or 10000, as long as the molecule can be produced by the techniques of those skilled in the art.

[0239] Alternatively, for example, the dissociation rate constant (kd) can be suitably used as an index for indicating the ratio of the antigen-binding activity of an antigen-binding domain or antigen-binding molecule comprising the domain of the present invention between that at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition and at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition. When kd (dissociation rate constant) is used as an index for indicating the binding activity ratio instead of KD (dissociation constant), the value of kd (in an acidic pH range condition)/kd (in a neutral pH range condition), which is the ratio of kd (dissociation rate constant) for the antigen at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition to kd (dissociation rate constant) at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, is preferably 2 or more, more preferably 5 or more, still more preferably 10 or more, and yet more preferably 30 or more. The upper limit of kd (in an acidic pH range condition)/kd (in a neutral pH range condition) value is not particularly limited, and may be any value such as 50, 100, or 200, as long as the molecule can be produced by the techniques of those skilled in the art.

[0240] When the antigen is a soluble antigen, the dissociation rate constant (kd) can be used as the value for antigen-binding activity and when the antigen is a membrane antigen, the apparent dissociation rate constant (kd) can be used. The dissociation rate constant (kd) and apparent dissociation rate constant (kd) can be determined by methods known to those skilled in the art, and Biacore (GE healthcare), flow cytometer, and such may be used. In the present invention, when the antigen-binding activity of an antigen-binding domain or antigen-binding molecule comprising the domain is measured at different hydrogen ion concentrations, i.e., pHs, conditions other than the hydrogen ion concentration, i.e., pH, are preferably the same.

[0241] For example, an antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition is lower than that at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, which is one embodiment provided by the present invention, can be obtained via screening of antigen-binding domains or antigen-binding molecules, comprising the following steps (a) to (c):

(a) obtaining the antigen-binding activity of an antigen-binding domain or antigen-binding molecule in an acidic pH range condition;
(b) obtaining the antigen-binding activity of an antigen-binding domain or antigen-binding molecule in a neutral pH range condition; and
(c) selecting an antigen-binding domain or antigen-binding molecule whose antigen-binding activity in the acidic pH range condition is lower than that in the neutral pH range condition.

[0242] Alternatively, an antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, is lower than that at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, which is one embodiment provided by the present invention, can be obtained via screening of antigen-binding domains or antigen-binding molecules, or a library thereof, comprising the following steps (a) to (c):

(a) contacting an antigen-binding domain or antigen-binding molecule, or a library thereof, in a neutral pH range condition with an antigen;
(b) placing in an acidic pH range condition the antigen-binding domain or antigen-binding molecule bound to the antigen in step (a); and
(c) isolating the antigen-binding domain or antigen-binding molecule dissociated in step (b).

[0243] An antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition is lower than that at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, which is another embodiment provided by the present invention, can be obtained via screening of antigen-binding domains or antigen-binding molecules, or a library thereof, comprising the following steps (a) to (d):

(a) contacting in an acidic pH range condition an antigen with a library of antigen-binding domains or antigen-binding molecules;
(b) selecting the antigen-binding domain or antigen-binding molecule which does not bind to the antigen in step (a);
(c) allowing the antigen-binding domain or antigen-binding molecule selected in step (b) to bind with the antigen in a neutral pH range condition; and
(d) isolating the antigen-binding domain or antigen-binding molecule bound to the antigen in step (c).

[0244] An antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, is lower than that at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, which is even another embodiment provided by the present invention, can be obtained by a screening method comprising the following steps (a) to (c):

(a) contacting in a neutral pH range condition a library of antigen-binding domains or antigen-binding molecules with a column immobilized with an antigen;
(b) eluting in an acidic pH range condition from the column the antigen-binding domain or antigen-binding molecule bound to the column in step (a); and
(c) isolating the antigen-binding domain or antigen-binding molecule eluted in step (b).

[0245] An antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, is lower than that at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, which is still another embodiment provided by the present invention, can be obtained by a screening method comprising the following steps (a) to (d):

(a) allowing, in an acidic pH range condition, a library of antigen-binding domains or antigen-binding molecules to pass a column immobilized with an antigen;
(b) collecting the antigen-binding domain or antigen-binding molecule eluted without binding to the column in step (a);
(c) allowing the antigen-binding domain or antigen-binding molecule collected in step (b) to bind with the antigen in a neutral pH range condition; and
(d) isolating the antigen-binding domain or antigen-binding molecule bound to the antigen in step (c).

[0246] An antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, is lower than that at a low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, which is yet another embodiment provided by the present invention, can be obtained by a screening method comprising the following steps (a) to (d):

(a) contacting an antigen with a library of antigen-binding domains or antigen-binding molecules in a neutral pH range condition;
(b) obtaining the antigen-binding domain or antigen-binding molecule bound to the antigen in step (a);
(c) placing in an acidic pH range condition the antigen-binding domain or antigen-binding molecule obtained in step (b); and
(d) isolating the antigen-binding domain or antigen-binding molecule whose antigen-binding activity in step (c) is weaker than the standard selected in step (b).

[0247] The above-described steps may be repeated twice or more times. Thus, the present invention provides antigen-binding domains and antigen-binding molecules whose antigen-binding activity in an acidic pH range condition is lower than that in a neutral pH range condition, which are obtained by a screening method that further comprises the steps of repeating steps (a) to (c) or (a) to (d) in the above-described screening methods. The number of times that steps (a) to (c) or (a) to (d) is repeated is not particularly limited; however, the number is 10 or less in general.

[0248] In the screening methods of the present invention, the antigen-binding activity of an antigen-binding domain or antigen-binding molecule at a condition of a high hydrogen ion concentration or low pH, i.e., in an acidic pH range, is not particularly limited, as long as it is the antigen-binding activity at a pH of between 4.0 and 6.5, and includes the antigen-binding activity at a pH of between 4.5 and 6.6 as the preferred pH. The antigen-binding activity also includes that at a pH of between 5.0 and 6.5, and that at a pH of between 5.5 and 6.5 as another preferred pH. The antigen-binding activity also includes that at the pH in the early endosome in vivo as the more preferred pH, and specifically, that at pH 5.8. Meanwhile, the antigen-binding activity of an antigen-binding domain or antigen-binding molecule at a condition of a low hydrogen ion concentration or high pH, i.e., in a neutral pH range, is not particularly limited, as long as it is the antigen-binding activity at a pH of between 6.7 and 10, and includes the antigen-binding activity at a pH of between 6.7 and 9.5 as the preferred pH. The antigen-binding activity also includes that at a pH of between 7.0 and 9.5 and that at a pH of between 7.0 and 8.0 as another preferred pH. The antigen-binding activity also includes that at the pH of plasma in vivo as the more preferred pH, and specifically, that at pH 7.4.

[0249] The antigen-binding activity of an antigen-binding domain or antigen-binding molecule can be measured by methods known to those skilled in the art. Those skilled in the art can suitably determine conditions other than ionized calcium concentration. The antigen-binding activity of an antigen-binding domain or antigen-binding molecule can be assessed based on the dissociation constant (KD), apparent dissociation constant (KD), dissociation rate constant (kd), apparent dissociation rate constant (kd), and such. These can be determined by methods known to those skilled in the art, for example, using Biacore (GE healthcare), Scatchard plot, or FACS.

[0250] In the present invention, the step of selecting an antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, is higher than that at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, is synonymous with the step of selecting an antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, is lower than that at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition.

[0251] As long as the antigen-binding activity at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, is higher than that at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, the difference between the antigen-binding activity at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, and that at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, is not particularly limited; however, the antigen-binding activity at a condition of low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, is preferably twice or more, more preferably 10 times or more, and still more preferably 40 times or more than that at a condition of high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition.

[0252] The antigen-binding domain or antigen-binding molecule whose antigen-binding activity varies depending on hydrogen ion concentration conditions of the present invention to be screened by the above-described screening methods may be prepared in any manner. For example, conventional antigen-binding molecules, conventional libraries (phage library, etc.), antibodies or libraries prepared from B cells of immunized animals or from hybridomas obtained by immunizing animals, antibodies or libraries (libraries with increased content of amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids, libraries introduced with amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acid mutations at specific positions, etc.) obtained by introducing amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acid mutations into the above-described antibodies or libraries may be used.

[0253] Methods for obtaining an antigen-binding domain or antigen-binding molecule whose antigen-binding activity at a low hydrogen ion concentration or high pH, i.e., in a neutral pH range condition, is higher than that at a high hydrogen ion concentration or low pH, i.e., in an acidic pH range condition, from an antigen-binding domains or antigen-binding molecules prepared from hybridomas obtained by immunizing animals or from B cells of immunized animals preferably include, for example, the antigen-binding molecule or antigen-binding molecule in which at least one of the amino acids of the antigen-binding domain or antigen-binding molecule is substituted with an amino acid with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or an unnatural amino acid mutation, or the antigen-binding domain or antigen-binding molecule inserted with an amino acid with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acid, such as those described in WO 2009/125825.

[0254] The sites of introducing mutations of amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids are not particularly limited, and may be any position as long as the antigen-binding activity in an acidic pH range becomes weaker than that in a neutral pH range (the value of KD (in an acidic pH range)/KD (in a neutral pH range) or kd (in an acidic pH range)/kd (in a neutral pH range) is increased) as compared to before substitution or insertion. For example, when the antigen-binding molecule is an antibody, antibody variable region and CDRs are suitable. Those skilled in the art can appropriately determine the number of amino acids to be substituted with or the number of amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids to be inserted. It is possible to substitute with a single amino acid having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or a single unnatural amino acid; it is possible to insert a single amino acid having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or a single unnatural amino acid; it is possible to substitute with two or more amino acids having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or two or more unnatural amino acids; and it is possible to insert two or more amino acids having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or two or more unnatural amino acids. Alternatively, other amino acids can be deleted, added, inserted, and/or substituted concomitantly, aside from the substitution into amino acids having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids, or the insertion of amino acids having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids. Substitution into or insertion of amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids can performed randomly by methods such as histidine scanning, in which the alanine of alanine scanning known to those skilled in the art is replaced with histidine. Antigen-binding molecules exhibiting a greater value of KD (in an acidic pH range)/KD (in a neutral pH range) or kd (in an acidic pH range)/kd (in a neutral pH range) as compared to before the mutation can be selected from antigen-binding domains or antibodies introduced with random insertions or substitution mutations of amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids.

[0255] Preferred examples of antigen-binding molecules containing the mutation into amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids as described above and whose antigen-binding activity in an acidic pH range is lower than that in a neutral pH range include, antigen-binding molecules whose antigen-binding activity in the neutral pH range after the mutation into amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids is comparable to that before the mutation into amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids. Herein, “an antigen-binding molecule after the mutation with amino acids having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids has an antigen-binding activity comparable to that before the mutation with amino acids having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids” means that, when taking the antigen-binding activity of an antigen-binding molecule before the mutation with amino acids having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids as 100%, the antigen-binding activity of an antigen-binding molecule after the mutation with amino acids having a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids is at least 10% or more, preferably 50% or more, more preferably 80% or more, and still more preferably 90% or more. The antigen-binding activity after the mutation of amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids at pH 7.4 may be higher than that before the mutation of amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids at pH 7.4. If the antigen-binding activity of an antigen-binding molecule is decreased due to insertion of or substitution into amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids, the antigen-binding activity can be made to be comparable to that before the insertion of or substitution into amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids, by introducing a substitution, deletion, addition, and/or insertion of one or more amino acids of the antigen-binding molecule. The present invention also includes antigen-binding molecules whose binding activity has been adjusted to be comparable by substitution, deletion, addition, and/or insertion of one or more amino acids after substitution or insertion of amino acids with a side chain pKa of 4.0-8.0 (for example, histidine and glutamic acid) or unnatural amino acids.

[0256] In one embodiment of the present invention, a library containing multiple antigen-binding domains or antigen-binding molecules of the present invention whose sequences are different from one another can also be constructed by combining heavy chain variable regions, produced as a randomized variable region sequence library, with light chain variable regions introduced with “at least one amino acid residue that changes the antigen-binding activity of antigen-binding domain or antigen-binding molecule depending on the hydrogen ion concentration condition”.

[0257] Such amino acid residues include, but are not limited to, for example, amino acid residues contained in the light chain CDR1. The amino acid residues also include, but are not limited to, for example, amino acid residues contained in the light chain CDR2. The amino acid residues also include, but are not limited to, for example, amino acid residues contained in the light chain CDR3.

[0258] The above-described amino acid residues contained in the light chain CDR1 include, but are not limited to, for example, amino acid residue(s) of position(s) 24, 27, 28, 31, 32, and/or 34 according to Kabat numbering in the CDR1 of light chain variable region. Meanwhile, the amino acid residues contained in the light chain CDR2 include, but are not limited to, for example, amino acid residue(s) of position(s) 50, 51, 52, 53, 54, 55, and/or 56 according to Kabat numbering in the CDR2 of light chain variable region. Furthermore, the amino acid residues in the light chain CDR3 include, but are not limited to, for example, amino acid residues of position(s) 89, 90, 91, 92, 93, 94, and/or 95A according to Kabat numbering in the CDR3 of light chain variable region. Moreover, the amino acid residues can be contained alone or can be contained in combination of two or more amino acids as long as they allow the change in the antigen-binding activity of an antigen-binding molecule depending on the hydrogen ion concentration condition.

[0259] Even when the heavy chain variable region produced as a randomized variable region sequence library is combined with the above-described light chain variable region introduced with “at least one amino acid residue that changes the antigen-binding activity of an antigen-binding molecule depending on the hydrogen ion concentration condition”, it is possible to design so that the flexible residues are contained in the sequence of the light chain variable region in the same manner as described above. The number and position of the flexible residues are not particularly limited to a specific embodiment, as long as the antigen-binding activity of antigen-binding domain or antigen-binding molecule of the present invention changes depending on the hydrogen ion concentration condition. Specifically, the CDR and/or FR sequences of heavy chain and/or light chain can contain one or more flexible residues. For example, flexible residues to be introduced into the sequences of the light chain variable regions include, but are not limited to, for example, the amino acid residues listed in Tables 3 and 4. Meanwhile, amino acid sequences of light chain variable regions other than the flexible residues and amino acid residues that change the antigen-binding activity of an antigen-binding domain or antigen-binding molecule depending on the hydrogen ion concentration condition suitably include, but are not limited to, germ line sequences such as Vk1 (SEQ ID NO: 46), Vk2 (SEQ ID NO: 47), Vk3 (SEQ ID NO: 48), and Vk4 (SEQ ID NO: 49).

TABLE 3
POSITION	AMINO ACID
CDR1
28	S: 100%
29	I: 100%
30	N: 25%	S: 25%	R: 25%	H: 25%
31	S: 100%
32	H: 100%
33	L: 100%
34	A: 50%	N: 50%
CDR2
50	H: 100%				OR	A: 25%	D: 25%	G: 25%	K: 25%
51	A: 100%					A: 100%
52	S: 100%					S: 100%
53	K: 33.3%	N: 33.3%	S: 33.3%			H: 100%
54	L: 100%					L: 100%
55	Q: 100%					Q: 100%
56	S: 100%					S: 100%
CDR3
90	Q: 100%				OR	Q: 100%
91	H: 100%					S: 33.3%	R: 33.3%	Y: 33.3%
92	G: 25%	N: 25%	S: 25%	Y: 25%		H: 100%
93	H: 33.3%	N: 33.3%	S: 33.3%			H: 33.3%	N: 33.3%	S: 33.3%
94	S: 50%	Y: 50%				S: 50%	Y: 50%
95	P: 100%					P: 100%
96	L: 50%	Y: 50%				L: 50%	Y: 50%
(Position indicates Kabat numbering)

TABLE 4
CDR	POSITION	AMINO ACID
CDR1	28	S: 100%
	29	I: 100%
	30	H: 30%	N: 10%	S: 50%	R: 1.0%
	31	N: 35%	S: 65%
	32	H: 40%	N: 20%	Y: 40%
	33	L: 100%
	34	A: 70%	N: 30%
CDR2	50	A: 25%	D: 15%	G: 25%	H: 30%	K: 5%
	51	A: 100%
	52	S: 100%
	53	H: 30%	K: 10%	N: 15%	S: 45%
	54	L: 100%
	55	Q: 100%
	56	S: 100%
CDR3	90	Q: 100%
	91	H: 30%	S: 15%	R: 10%	Y: 45%
	92	G: 20%	H: 30%	N: 20%	S: 15%	Y: 15%
	93	H: 30%	N: 25%	S: 45%
	94	S: 50%	Y: 50%
	95	P: 100%
	96	L: 50%	Y: 50%
(Position indicates Kabat numbering)

[0260] Any amino acid residue may be suitably used as the above-described amino acid residues that change the antigen-binding activity of an antigen-binding domain or antigen-binding molecule depending on the hydrogen ion concentration conditions. Specifically, such amino acid residues include amino acids with a side chain pKa of 4.0-8.0. Such electron-releasing amino acids preferably include, for example, naturally occurring amino acids such as histidine and glutamic acid, as well as unnatural amino acids such as histidine analogs (US2009/0035836), m-NO2-Tyr (pKa 7.45), 3,5-Br2-Tyr (pKa 7.21), and 3,5-12-Tyr (pKa 7.38) (Bioorg. Med. Chem. (2003) 11 (17), 3761-3768). Particularly preferred amino acid residues include, for example, amino acids with a side chain pKa of 6.0-7.0. Such electron-releasing amino acid residues preferably include, for example, histidine.

[0261] The preferred heavy chain variable region that is used in combination includes, for example, randomized variable region libraries. Known methods are appropriately combined as a method for producing a randomized variable region library. In a non-limiting embodiment of the present invention, an immune library constructed based on antibody genes derived from animals immunized with specific antigens, patients with infection or persons with an elevated antibody titer in blood as a result of vaccination, cancer patients, or lymphocytes of autoimmune diseases may be suitably used as a randomized variable region library.

[0262] In another non-limiting embodiment of the present invention, in the same manner as described above, a synthetic library in which the CDR sequences of V genes from genomic DNA or functional reconstructed V genes are replaced with a set of synthetic oligonucleotides containing the sequences encoding codon sets of an appropriate length can also be suitably used as a randomized variable region library. In this case, the CDR3 sequence alone may be replaced because variety in the gene sequence of heavy chain CDR3 is observed. The basis for giving rise to amino acid variations in the variable region of an antigen-binding molecule is to generate variations of amino acid residues of surface-exposed positions of the antigen-binding molecule. The surface-exposed position refers to a position where an amino acid is exposed on the surface and/or contacted with an antigen based on the conformation, structural ensemble, and/or modeled structure of an antigen-binding molecule, and in general, such positions are the CDRs. The surface-exposed positions are preferably determined using the coordinates derived from a three-dimensional model of the antigen-binding molecule using computer programs such as InsightII program (Accelrys). The surface-exposed positions can be determined using algorithms known in the art (for example, Lee and Richards (J. Mol. Biol. (1971) 55, 379-400); Connolly (J. Appl. Cryst. (1983) 16, 548-558)). The surface-exposed positions can be determined based on the information on the three dimensional structure of antibodies using software suitable for protein modeling. Software which is suitably used for this purpose includes the SYBYL biopolymer module software (Tripos Associates). When the algorithm requires the input size parameter from the user, the “size” of probe for use in computation is generally or preferably set at about 1.4 angstrom or less in radius. Furthermore, a method for determining surface-exposed region and area using personal computer software is described by Pacios (Comput. Chem. (1994) 18 (4), 377-386; and J. Mol. Model. (1995) 1, 46-53).

[0263] In still another non-limiting embodiment of the present invention, a naive library constructed from antibody genes derived from lymphocytes of healthy persons and comprising naive sequences, which are unbiased repertoire of antibody sequences, can also be particularly suitably used as a randomized variable region library (Gejima et al. (Human Antibodies (2002) 11, 121-129); and Cardoso et al. (Scand. J. Immunol. (2000) 51, 337-344)).

[0264] Furthermore, amino acid alterations for enhancing the human FcRn-binding activity under an acidic pH-range condition can be combined in addition. More specifically, alterations used to enhance human FcRn-binding activity under an acidic pH range condition may be carried out on an IgG antibody, for example, by a method of substituting Leu for Met at position 428, and substituting Ser for Asn at position 434, according to EU numbering (Nat Biotechnol, 2010 28: 157-159); a method of substituting Ala for Asn at position 434 (Drug Metab Dispos. 2010 April; 38(4): 600-5); a method of substituting Tyr for Met at position 252, substituting Thr for Ser at position 254, and substituting Glu for Thr at position 256 (J Biol Chem, 2006, 281: 23514-23524); a method for substituting Gln for Thr at position 250, and substituting Leu for Met at position 428 (J Immunol. 2006, 176(1): 346-56); method of substituting His for Asn at position 434 (Clinical Pharmacology & Therapeutics (2011) 89(2): 283-290), or by using alterations such as those described in WO2010106180, WO2010045193, WO2009058492, WO2008022152, WO2006050166, WO2006053301, WO2006031370, WO2005123780, WO2005047327, WO2005037867, WO2004035752, WO2002060919, or such.

[0265] Furthermore, an antibody molecule produced by substituting His for Asn at position 434 (EU numbering) in humanized anti-CD4 antibody to enhance human FcRn-binding activity under an acidic pH range condition and to improve plasma retention properties was recently reported to bind to rheumatoid factors (RF) (Clin Pharmacol Ther. 2011 February; 89(2): 283-90). This antibody has a human IgG1 Fc region, but by substituting His for Asn at position 434 which is positioned at the FcRn-binding site, it has been shown to bind to rheumatoid factors that recognize this substituted site.

[0266] As described above, various alterations have been reported as alterations for enhancing human FcRn-binding activity under an acidic pH range condition; however, by introducing these alterations into the FcRn-binding site in an Fc region, affinity to rheumatoid factors which recognize this site may become enhanced.

[0267] However, by introducing alterations which do not reduce FcRn-binding activity and reduce only binding activity to rheumatoid factors into the site in the Fc region, antigen-binding molecules with enhanced human FcRn-binding activity under an acidic pH range condition and without affinity to rheumatoid factors can be produced.

[0268] For alterations that reduce binding activity to rheumatoid factors, alterations to positions 248-257, 305-314, 342-352, 380-386, 388, 414-421, 423, 425-437, 439, and 441-444 according to EU numbering are used. Preferably, alterations to positions 387, 422, 424, 426, 433, 436, 438, and 440 are used. Particularly preferably, alteration of substituting Glu or Ser for Val at position 422, alteration of substituting Arg for Ser at position 424, alteration of substituting Asp for His at position 433, alteration of substituting Thr for Tyr at position 436, alteration of substituting Arg or Lys for Gln at position 438, and alteration of substituting Glu or Asp for Ser at position 440 are used. These alterations may be used alone or by combining alterations at multiple positions.

[0269] Alternatively, to decrease binding activity to rheumatoid factors, an N-type glycosylation sequence may be introduced into this site. Specifically, Asn-Xxx-Ser/Thr (Xxx is any amino acid other than Pro) is known as an N-type glycosylation sequence. Adding an N-type sugar chain by introducing this sequence into the site in the Fc region enables inhibition of binding to RF by steric hindrance due to the N-type sugar chain. Alterations used to add an N-type sugar chain are preferably alteration which substitutes Asn for Lys at position 248, alteration which substitutes Asn for Ser at position 424, alteration which substitutes Asn for Tyr at position 436 and substitutes Thr for Gln at position 438, and alteration which substitutes Asn for Gln at position 438. Particularly preferably, the alteration which substitutes Asn for Ser at position 424 is used.

[0270] A preferred example of a polypeptide comprising an Fc region variant of the present invention includes at least one of polypeptides comprising two associated Fc region variants like an IgG antibody. When an IgG antibody is used as the antibody, the type of constant region is not limited, and an IgG isotype (subclasses) such as IgG1, IgG2, IgG3, and IgG4 can be used. IgG antibodies of the present invention are preferably human IgGs, and more preferably human IgG1 and human IgG4. The amino acid sequences of the heavy-chain constant regions of human IgG1 and human IgG4 are known. A plurality of allotype sequences due to genetic polymorphisms have been described in the Sequences of Proteins of Immunological Interest, NIH Publication No. 91-3242 for the human IgG1 constant region, and any of the sequences may be used in the present invention.

[0271] In the present invention, amino acid alteration means any of substitution, deletion, addition, insertion, and modification, or a combination thereof. In the present invention, amino acid alteration may be rephrased as amino acid mutation, and they are used synonymously.

[0272] When substituting amino acid residues, substitution to a different amino acid residue is carried out with the objective of altering aspects such as (a)-(c) described below:

(a) polypeptide backbone structure in the sheet-structure or helical-structure region;
(b) electric charge or hydrophobicity at the target site; or
(c) size of the side chain.

[0273] Amino acid residues are classified into the following groups based on their general side chain properties:

(1) hydrophobic: norleucine, met, ala, val, leu, and ile;
(2) neutral hydrophilic: cys, ser, thr, asn, and gln;
(3) acidic: asp and glu;
(4) basic: his, lys, and arg;
(5) residues that affect the chain orientation: gly and pro; and
(6) aromatic: tip, tyr, and phe.

[0274] Substitution between amino acid residues within each of these amino acid groups is referred to as conservative substitution, and amino acid residue substitution between different groups is referred to as non-conservative substitution. Substitutions in the present invention may be conservative substitutions or non-conservative substitutions, or a combination of conservative substitutions and non-conservative substitutions.

[0275] Amino acid sequence alterations are produced by various methods known to those skilled in the art. Such methods include the site-directed mutagenesis method (Hashimoto-Gotoh, T, Mizuno, T, Ogasahara, Y, and Nakagawa, M. (1995) An oligodeoxyribonucleotide-directed dual amber method for site-directed mutagenesis. Gene 152: 271-275; Zoller, M J, and Smith, M. (1983) Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. Methods Enzymol. 100: 468-500; Kramer, W, Drutsa, V, Jansen, H W, Kramer, B, Pflugfelder, M, and Fritz, H J (1984) The gapped duplex DNA approach to oligonucleotide-directed mutation construction. Nucleic Acids Res. 12: 9441-9456; Kramer W, and Fritz H J (1987) Oligonucleotide-directed construction of mutations via gapped duplex DNA Methods. Enzymol. 154, 350-367; and Kunkel, TA (1985) Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci USA. 82: 488-492), the PCR mutation method, and the cassette mutation method, but are not limited thereto.

[0276] Amino acid modification of the present invention includes post-translational modification. A specific post-translational modification may be addition or deletion of a sugar chain. For example, in the IgG1 constant region comprising the amino acid sequence of SEQ ID NO: 31, the amino acid residue at position 297 (EU numbering) may be sugar chain-modified. The sugar-chain structure for the modification is not limited. Generally, antibodies expressed in eukaryotic cells comprise glycosylation in the constant region. Therefore, antibodies expressed in cells such as those below are normally modified by some type of sugar chain:

• • [0277] antibody-producing cells of mammals
  • [0278] eukaryotic cells transformed with an expression vector comprising a DNA encoding an antibody

[0279] Eukaryotic cells shown here include yeast and animal cells. For example, CHO cells and HEK293H cells are representative animal cells used in transformation with an expression vector comprising an antibody-encoding DNA. On the other hand, those without glycosylation at this site are also included in the constant region of the present invention. Antibodies whose constant region is not glycosylated can be obtained by expressing an antibody-encoding gene in prokaryotic cells such as Escherichia coli.

[0280] Specifically, for example, sialic acid may be added to the sugar chain of an Fc region (MAbs. 2010 September-October; 2(5): 519-27).

[0281] Furthermore, the present invention provides antibodies comprising any of Fc region variant described above.

[0282] The term “antibody/antibodies” in the present invention is used in the broadest sense, and as long as the desired biological activity is shown, it comprises any antibody such as monoclonal antibodies (including full-length monoclonal antibodies), polyclonal antibodies, antibody variants, antibody fragments, polyspecific antibodies (multi-specific antibodies) (for example, bispecific antibodies (diabodies)), chimeric antibodies, and humanized antibodies.

[0283] Regarding the antibodies of the present invention, the antigen type and antibody origin are not limited, and they may be any type of antibodies. The origin of the antibodies is not particularly limited, but examples include human antibodies, mouse antibodies, rat antibodies, and rabbit antibodies.

[0284] Methods for producing the antibodies are well known to those skilled in the art, and for example, monoclonal antibodies may be produced by the hybridoma method (Kohler and Milstein, Nature 256: 495 (1975)), or the recombination method (U.S. Pat. No. 4,816,567). Alternatively, they may be isolated from a phage antibody library (Clackson et al., Nature 352: 624-628 (1991); Marks et al., J. Mol. Biol. 222: 581-597 (1991)).

[0285] A humanized antibody is also called a reshaped human antibody. Specifically, humanized antibodies prepared by grafting the CDRs of a non-human animal antibody such as a mouse antibody to a human antibody and such are known. Common genetic engineering techniques for obtaining humanized antibodies are also known. Specifically, for example, overlap extension PCR is known as a method for grafting mouse antibody CDRs to human FRs.

[0286] A vector for expressing a humanized antibody can be produced by inserting a DNA encoding an antibody variable region in which three CDRs and four FRs are ligated and a DNA encoding a human antibody constant region into an expression vector so that these DNAs are fused in frame. After this integration vector is transfected into a host to establish recombinant cells, these cells are cultured, and the DNA encoding the humanized antibody is expressed to produce the humanized antibody in the culture of the cells (see, European Patent Publication No. EP 239,400, and International Patent Publication No. WO 1996/002576).

[0287] As necessary, an amino acid residue in an FR may be substituted so that the CDRs of a reshaped human antibody form an appropriate antigen-binding site. For example, a mutation can be introduced into the amino acid sequence of an FR by applying the PCR method used for grafting mouse CDRs to human FRs.

[0288] A desired human antibody can be obtained by DNA immunization using a transgenic animal having the complete repertoire of human antibody genes (see International Publication Nos. WO 1993/012227, WO 1992/003918, WO 1994/002602, WO 1994/025585, WO 1996/034096, and WO 1996/033735) as an animal for immunization.

[0289] Furthermore, technologies for obtaining a human antibody by panning using a human antibody library are known. For example, a human antibody V region is expressed on the surface of a phage as a single-chain antibody (scFv) by the phage display method. The scFv-expressing phage that binds to the antigen can be selected. The DNA sequence that encodes the V region of the antigen-bound human antibody can be determined by analyzing the genes of the selected phage. After determining the DNA sequence of the scFv that binds to the antigen, an expression vector can be prepared by fusing the V-region sequence in-frame with the sequence of a desired human antibody C region, and then inserting this into a suitable expression vector. The expression vector is introduced into suitable expression cells such as those described above, and the human antibody can be obtained by expressing the human antibody-encoding gene. These methods are already known (see, International Publication Nos. WO 1992/001047, WO 1992/020791, WO 1993/006213, WO 1993/011236, WO 1993/019172, WO 1995/001438, and WO 1995/15388).

[0290] Variable regions constituting the antibodies of the present invention can be variable regions that recognize any antigen.

[0291] Herein, there is no particular limitation on the antigen, and it may be any antigens. Examples of such antigens preferably include ligands (cytokines, chemokines, and such), receptors, cancer antigens, MHC antigens, differentiation antigens, immunoglobulins, and immune complexes partly containing immunoglobulins.

[0292] Examples of cytokines include interleukins 1 to 18, colony stimulating factors (G-CSF, M-CSF, GM-CSF, etc.), interferons (IFN-α, IFN-β, IFN-γ, etc.), growth factors (EGF, FGF, IGF, NGF, PDGF, TGF, HGF, etc.), tumor necrosis factors (TNF-α and TNF-β), lymphotoxin, erythropoietin, leptin, SCF, TPO, MCAF, and BMP.

[0293] Examples of chemokines include CC chemokines such as CCL1 to CCL28, CXC chemokines such as CXCL1 to CXCL17, C chemokines such as XCL1 to XCL2, and CX3C chemokines such as CX3CL1.

[0294] Examples of receptors include receptors belonging to receptor families such as the hematopoietic growth factor receptor family, cytokine receptor family, tyrosine kinase-type receptor family, serine/threonine kinase-type receptor family, TNF receptor family, G protein-coupled receptor family, GPI anchor-type receptor family, tyrosine phosphatase-type receptor family, adhesion factor family, and hormone receptor family. The receptors belonging to these receptor families and their characteristics have been described in many documents such as Cooke B A., King R J B., van der Molen H J. ed. New Comprehesive Biochemistry Vol. 18B “Hormones and their Actions Part II” pp. 1-46 (1988) Elsevier Science Publishers BV; Patthy (Cell (1990) 61 (1): 13-14); Ullrich et al. (Cell (1990) 61 (2): 203-212); Massagué (Cell (1992) 69 (6): 1067-1070); Miyajima et al. (Annu. Rev. Immunol. (1992) 10: 295-331); Taga et al. (FASEB J. (1992) 6, 3387-3396); Fantl et al. (Annu. Rev. Biochem. (1993), 62: 453-481); Smith et al. (Cell (1994) 76 (6): 959-962); and Flower D R. Flower (Biochim. Biophys. Acta (1999) 1422 (3): 207-234).

[0295] Examples of specific receptors belonging to the above-mentioned receptor families preferably include human or mouse erythropoietin (EPO) receptors (Blood (1990) 76 (1): 31-35; and Cell (1989) 57 (2): 277-285), human or mouse granulocyte-colony stimulating factor (G-CSF) receptors (Proc. Natl. Acad. Sci. USA. (1990) 87 (22): 8702-8706, mG-CSFR; Cell (1990) 61 (2): 341-350), human or mouse thrombopoietin (TPO) receptors (Proc Natl Acad Sci USA. (1992) 89 (12): 5640-5644; EMBO J. (1993) 12(7): 2645-53), human or mouse insulin receptors (Nature (1985) 313 (6005): 756-761), human or mouse Flt-3 ligand receptors (Proc. Natl. Acad. Sci. USA. (1994) 91 (2): 459-463), human or mouse platelet-derived growth factor (PDGF) receptors (Proc. Natl. Acad. Sci. USA. (1988) 85 (10): 3435-3439), human or mouse interferon (IFN)-α and β receptors (Cell (1990) 60 (2): 225-234; and Cell (1994) 77 (3): 391-400), human or mouse leptin receptors, human or mouse growth hormone (GH) receptors, human or mouse interleukin (IL)-10 receptors, human or mouse insulin-like growth factor (IGF)-I receptors, human or mouse leukemia inhibitory factor (LIF) receptors, and human or mouse ciliary neurotrophic factor (CNTF) receptors.

[0296] Cancer antigens are antigens that are expressed as cells become malignant, and they are also called tumor-specific antigens. Abnormal sugar chains that appear on cell surfaces or protein molecules when cells become cancerous are also cancer antigens, and they are also called sugar-chain cancer antigens. Examples of cancer antigens preferably include GPC3 which is a receptor belonging to the GPI anchor-type receptor family mentioned above, and is also expressed in several cancers including liver cancer (Int J Cancer. (2003) 103 (4): 455-65), as well as EpCAM which is expressed in several cancers including lung cancer (Proc Natl Acad Sci USA. (1989) 86 (1): 27-31), CA19-9, CA15-3, and sialyl SSEA-1 (SLX).

[0297] MHC antigens are roughly classified into MHC class I antigens and MHC class II antigens. MHC class I antigens include HLA-A, -B, -C, -E, -F, -G, and -H, and MHC class II antigens include HLA-DR, -DQ, and -DP.

[0298] Differentiation antigens may include CD1, CD2, CD4, CD5, CD6, CD7, CD8, CD10, CD11a, CD11b, CD11c, CD13, CD14, CD15s, CD16, CD18, CD19, CD20, CD21, CD23, CD25, CD28, CD29, CD30, CD32, CD33, CD34, CD35, CD38, CD40, CD41a, CD41b, CD42a, CD42b, CD43, CD44, CD45, CD45RO, CD48, CD49a, CD49b, CD49c, CD49d, CD49e, CD49f, CD51, CD54, CD55, CD56, CD57, CD58, CD61, CD62E, CD62L, CD62P, CD64, CD69, CD71, CD73, CD95, CD102, CD106, CD122, CD126, and CDw130.

[0299] Immunoglobulins include IgA, IgM, IgD, IgG, and IgE Immune complexes include a component of at least any of the immunoglobulins.

[0300] Other antigens include, for example, the molecules below: 17-IA, 4-1BB, 4Dc, 6-keto-PGF1a, 8-iso-PGF2a, 8-oxo-dG, A1 adenosine receptor, A33, ACE, ACE-2, activin, activin A, activin AB, activin B, activin C, activin RIA, activin RIA ALK-2, activin RIB ALK-4, activin RIIA, activin RIIB, ADAM, ADAM10, ADAM12, ADAM15, ADAM17/TACE, ADAM8, ADAM9, ADAMTS, ADAMTS4, ADAMTS5, addressin, aFGF, ALCAM, ALK, ALK-1, ALK-7,alpha-1-antitrypsin, alpha-V/beta-1 antagonist, ANG, Ang, APAF-1, APE, APJ, APP, APRIL, AR, ARC, ART, artemin, anti-Id, ASPARTIC, atrial natriuretic peptide, av/b3 integrin, Ax1, b2M, B7-1, B7-2, B7-H, B-lymphocyte stimulating factor (BlyS), BACE, BACE-1, Bad, BAFF, BAFF-R, Bag-1, BAK, Bax, BCA-1, BCAM, Bc1, BCMA, BDNF, b-ECGF, bFGF, BID, Bik, BIM, BLC, BL-CAM, BLK, BMP, BMP-2 BMP-2a, BMP-3 Osteogenin, BMP-4 BMP-2b, BMP-5, BMP-6 Vgr-1, BMP-7 (OP-1), BMP-8 (BMP-8a, OP-2), BMPR, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BRK-2, RPK-1, BMPR-II (BRK-3), BMP, b-NGF, BOK, bombesin, bone-derived neurotrophic factor, BPDE, BPDE-DNA, BTC, complement factor 3 (C3), C3a, C4, C5, C5a, C10, CA125, CAD-8, calcitonin, cAMP, carcinoembryonic antigen (CEA), cancer associated antigen, cathepsin A, cathepsin B, cathepsin C/DPPI, cathepsin D, cathepsin E, cathepsin H, cathepsin L, cathepsin O, cathepsin S, cathepsin V, cathepsin X/Z/P, CBL, CCI, CCK2, CCL, CCL1, CCL11, CCL12, CCL13, CCL14, CCL15, CCL16, CCL17, CCL18, CCL19, CCL2, CCL20, CCL21, CCL22, CCL23, CCL24, CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL6, CCL7, CCL8, CCL9/10, CCR, CCR1, CCR10, CCR10, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD1, CD2, CD3, CD3E, CD4, CD5, CD6, CD7, CD8, CD10, CD11a, CD11b, CD11c, CD13, CD14, CD15, CD16, CD18, CD19, CD20, CD21, CD22, CD23, CD25, CD27L, CD28, CD29, CD30, CD30L, CD32, CD33 (p67 protein), CD34, CD38, CD40, CD40L, CD44, CD45, CD46, CD49a, CD52, CD54, CD55, CD56, CD61, CD64, CD66e, CD74, CD80 (B7-1), CD89, CD95, CD123, CD137, CD138, CD140a, CD146, CD147, CD148, CD152, CD164, CEACAM5, CFTR, cGMP, CINC, Botulinum toxin, Clostridium perfringens toxin, CKb8-1, CLC, CMV, CMV UL, CNTF, CNTN-1, COX, C-Ret, CRG-2, CT-1, CTACK, CTGF, CTLA-4, CX3CL1, CX3CR1, CXCL, CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6, CXCL7, CXCL8, CXCL9, CXCL10, CXCL11, CXCL12, CXCL13, CXCL14, CXCL15, CXCL16, CXCR, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, cytokeratin tumor associated antigen, DAN, DCC, DcR3, DC-SIGN, complement regulatory factor (Decay accelerating factor), des (1-3)-IGF-I (brain IGF-1), Dhh, digoxin, DNAM-1, Dnase, Dpp, DPPIV/CD26, Dtk, ECAD, EDA, EDA-A1, EDA-A2, EDAR, EGF, EGFR (ErbB-1), EMA, EMMPRIN, ENA, endothelin receptor, enkephalinase, eNOS, Eot, eotaxin 1, EpCAM, ephrin B2/EphB4, EPO, ERCC, E-selectin, ET-1, factor IIa, factor VII, factor VIIIc, factor IX, fibroblast activation protein (FAP), Fas, FcR1, FEN-1, ferritin, FGF, FGF-19, FGF-2, FGF3, FGF-8, FGFR, FGFR-3, fibrin, FL, FLIP, Flt-3, Flt-4, follicle stimulating hormone, fractalkine, FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, FZD9, FZD10, G250, Gash, GCP-2, GCSF, GD2, GD3, GDF, GDF-1, GDF-3 (Vgr-2), GDF-5 (BMP-14, CDMP-1), GDF-6 (BMP-13, CDMP-2), GDF-7 (BMP-12, CDMP-3), GDF-8 (myostatin), GDF-9, GDF-15 (MIC-1), GDNF, GDNF, GFAP, GFRa-1, GFR-alpha1, GFR-alpha2, GFR-alpha3, GITR, glucagon, Glut4, glycoprotein IIb/IIIa (GPIIb/IIIa), GM-CSF, gp130, gp72, GRO, growth hormone releasing hormone, hapten (NP-cap or NIP-cap), HB-EGF, HCC, HCMV gB envelope glycoprotein, HCMV gH envelope glycoprotein, HCMV UL, hematopoietic growth factor (HGF), Hep B gp120, heparanase, Her2, Her2/neu (ErbB-2), Her3 (ErbB-3), Her4 (ErbB-4), herpes simplex virus (HSV) gB glycoprotein, HSV gD glycoprotein, HGFA, high molecular weight melanoma-associated antigen (HMW-MAA), HIV gp120, HIV IIIB gp120 V3 loop, HLA, HLA-DR, HM1.24, HMFG PEM, HRG, Hrk, human cardiac myosin, human cytomegalovirus (HCMV), human growth hormone (HGH), HVEM, 1-309, IAP, ICAM, ICAM-1, ICAM-3, ICE, ICOS, IFNg, Ig, IgA receptor, IgE, IGF, IGF binding protein, IGF-1R, IGFBP, IGF-I, IGF-II, IL, IL-1, IL-1R, IL-2, IL-2R, IL-4, IL-4R, IL-5, IL-5R, IL-6, IL-6R, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-18, IL-18R, IL-23, interferon (INF)-alpha, INF-beta, INF-gamma, inhibin, iNOS, insulin A chain, insulin B chain, insulin-like growth factor1, integrin alpha2, integrin alpha3, integrin alpha4, integrin alpha4/beta1, integrin alpha4/beta7, integrin alpha5 (alpha V), integrin alpha5/beta1, integrin alpha5/beta3, integrin alpha6, integrin beta1, integrin beta2, interferon gamma, IP-10, I-TAC, JE, kallikrein 2, kallikrein 5, kallikrein 6, kallikrein 11, kallikrein 12, kallikrein 14, kallikrein 15, kallikrein L1, kallikrein L2, kallikrein L3, kallikrein L4, KC, KDR, keratinocyte growth factor (KGF), laminin 5, LAMP, LAP, LAP (TGF-1), latent TGF-1, latent TGF-1 bp1, LBP, LDGF, LECT2, lefty, Lewis-Y antigen, Lewis-Y associated antigen, LFA-1, LFA-3, Lfo, LIF, LIGHT, lipoprotein, LIX, LKN, Lptn, L-selectin, LT-a, LT-b, LTB4, LTBP-1, lung surface, luteinizing hormone, lymphotoxin beta receptor, Mac-1, MAdCAM, MAG, MAP2, MARC, MCAM, MCAM, MCK-2, MCP, M-CSF, MDC, Mer, METALLOPROTEASES, MGDF receptor, MGMT, MHC (HLA-DR), MIF, MIG, MIP, MIP-1-alpha, MK, MMAC1, MMP, MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-15, MMP-2, MMP-24, MMP-3, MMP-7, MMP-8, MMP-9, MPIF, Mpo, MSK, MSP, mucin (Muc1), MUC18, Mullerian-inhibiting substance, Mug, MuSK, NAIP, NAP, NCAD, N-C adherin, NCA 90, NCAM, NCAM, neprilysin, neurotrophin-3, -4, or -6, neurturin, nerve growth factor (NGF), NGFR, NGF-beta, nNOS, NO, NOS, Npn, NRG-3, NT, NTN, OB, OGG1, OPG, OPN, OSM, OX40L, OX40R, p150, p95, PADPr, parathyroid hormone, PARC, PARP, PBR, PBSF, PCAD, P-cadherin, PCNA, PDGF, PDGF, PDK-1, PECAM, PEM, PF4, PGE, PGF, PGI2, PGD2, PIN, PLA2, placental alkaline phosphatase (PLAP), PlGF, PLP, PP14, proinsulin, prorelaxin, protein C, PS, PSA, PSCA, prostate-specific membrane antigen (PSMA), PTEN, PTHrp, Ptk, PTN, R51, RANK, RANKL, RANTES, RANTES, relaxin A chain, relaxin B chain, renin, respiratory syncytial virus (RSV) F, RSV Fgp, Ret, Rheumatoid factor, RLIP76, RPA2, RSK, 5100, SCF/KL, SDF-1, SERINE, serum albumin, sFRP-3, Shh, SIGIRR, SK-1, SLAM, SLPI, SMAC, SMDF, SMOH, SOD, SPARC, Stat, STEAP, STEAP-II, TACE, TACI, TAG-72 (tumor-associated glycoprotein-72), TARC, TCA-3, T-cell receptor (for example, T-cell receptor alpha/beta), TdT, TECK, TEM1, TEM5, TEM7, TEM8, TERT, testis PLAP-like alkaline phosphatase, TfR, TGF, TGF-alpha, TGF-beta, TGF-beta Pan Specific, TGF-betaRl (ALK-5), TGF-betaRII, TGF-betaRIIb, TGF-betaRIII, TGF-beta1, TGF-beta2, TGF-beta3, TGF-beta4, TGF-beta5, thrombin, thymus Ck-1, thyroid-stimulating hormone, Tie, TIMP, TIQ, tissue factor, TMEFF2, Tmpo, TMPRSS2, TNF, TNF-alpha, TNF-alphabeta, TNF-beta2, TNFc, TNF-RI, TNF-RII, TNFRSF10A (TRAIL R1 Apo-2, DR4), TNFRSF10B (TRAIL R2 DR5, KILLER, TRICK-2A, TRICK-B), TNFRSF10C (TRAIL R3 DcR1, LIT, TRID), TNFRSF10D (TRAIL R4 DcR2, TRUNDD), TNFRSF11A (RANK ODF R, TRANCE R), TNFRSF11B (OPG OCIF, TR1), TNFRSF12 (TWEAK R FN14), TNFRSF13B (TACT), TNFRSF13C (BAFF R), TNFRSF14 (HVEM ATAR, HveA, LIGHT R, TR2), TNFRSF16 (NGFR p75NTR), TNFRSF17 (BCMA), TNFRSF18 (GITR AITR), TNFRSF19 (TROY TAJ, TRADE), TNFRSF19L (RELT), TNFRSF1A (TNF RI CD120a, p55-60), TNFRSF1B (TNF RII CD120b, p75-80), TNFRSF26 (TNFRH3), TNFRSF3 (LTbR TNF RIII, TNFC R), TNFRSF4 (OX40 ACT35, TXGP1 R), TNFRSF5 (CD40 p50), TNFRSF6 (Fas Apo-1, APT1, CD95), TNFRSF6B (DcR3 M68, TR6), TNFRSF7 (CD27), TNFRSF8 (CD30), TNFRSF9 (4-1BB CD137, ILA), TNFRSF21 (DR6), TNFRSF22 (DcTRAIL R2 TNFRH2), TNFRST23 (DcTRAIL R1 TNFRH1), TNFRSF25 (DR3 Apo-3, LARD, TR-3, TRAMP, WSL-1), TNFSF10 (TRAIL Apo-2 ligand, TL2), TNFSF11 (TRANCE/RANK ligand ODF, OPG ligand), TNFSF12 (TWEAK Apo-3 ligand, DR3 ligand), TNFSF13 (APRIL TALL2), TNFSF13B (BAFF BLYS, TALL1, THANK, TNFSF20), TNFSF14 (LIGHT HVEM ligand, LTg), TNFSF15 (TL1A/VEGI), TNFSF18 (GITR ligand AITR ligand, TL6), TNFSF1A (TNF-a Conectin, DIF, TNFSF2), TNFSF1B (TNF-b LTa, TNFSF1), TNFSF3 (LTb TNFC, p33), TNFSF4 (OX40 ligand gp34, TXGP1), TNFSF5 (CD40 ligand CD154, gp39, HIGM1, IMD3, TRAP), TNFSF6 (Fas ligand Apo-1 ligand, APT1 ligand), TNFSF7 (CD27 ligand CD70), TNFSF8 (CD30 ligand CD153), TNFSF9 (4-1BB ligand CD137 ligand), TP-1, t-PA, Tpo, TRAIL, TRAIL R, TRAIL-R1, TRAIL-R2, TRANCE, transferrin receptor, TRF, Trk, TROP-2, TSG, TSLP, tumor associated antigen CA125, tumor associated antigen expressing Lewis-Y associated carbohydrates, TWEAK, TXB2, Ung, uPAR, uPAR-1, urokinase, VCAM, VCAM-1, VECAD, VE-Cadherin, VE-cadherin-2, VEFGR-1 (flt-1), VEGF, VEGFR, VEGFR-3 (flt-4), VEGI, VIM, virus antigen, VLA, VLA-1, VLA-4, VNR integrin, von Willebrand factor, WIF-1, WNT1, WNT2, WNT2B/13, WNT3, WNT3A, WNT4, WNT5A, WNTSB, WNT6, WNT7A, WNT7B, WNT8A, WNT8B, WNT9A, WNT9A, WNT9B, WNT10A, WNT10B, WNT11, WNT16, XCL1, XCL2, XCR1, XCR1, XEDAR, XIAP, XPD, HMGB1, IgA, Aβ, CD81, CD97, CD98, DDR1, DKK1, EREG, Hsp90, IL-17/IL-17R, IL-20/IL-20R, oxidized LDL, PCSK9, prekallikrein, RON, TMEM16F, SOD1, Chromogranin A, Chromogranin B, tau, VAP1, high molecular weight kininogen, IL-31, IL-31R, Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.5, Nav1.6, Nav1.7, Nav1.8, Nav1.9, EPCR, C1, C1q, C1r, C1s, C2, C2a, C2b, C3, C3a, C3b, C4, C4a, C4b, C5, C5a, C5b, C6, C7, C8, C9, factor B, factor D, factor H, properdin, sclerostin, fibrinogen, fibrin, prothrombin, thrombin, tissue factor, factor V, factor Va, factor VII, factor VIIa, factor VIII, factor VIIIa, factor IX, factor IXa, factor X, factor Xa, factor XI, factor XIa, factor XII, factor XIIa, factor XIII, factor XIIIa, TFPI, antithrombin III, EPCR, thrombomodulin, TAPI, tPA, plasminogen, plasmin, PAI-1, PAI-2, GPC3, Syndecan-1, Syndecan-2, Syndecan-3, Syndecan-4, LPA, and S1P; and receptors for hormone and growth factors.

[0301] One or more amino acid residue alterations are allowed in the amino acid sequences constituting the variable regions as long as their antigen-binding activities are maintained. When altering a variable region amino acid sequence, there is no particularly limitation on the site of alteration and number of amino acids altered. For example, amino acids present in CDR and/or FR can be altered appropriately. When altering amino acids in a variable region, the binding activity is preferably maintained without particular limitation; and for example, as compared to before alteration, the binding activity is 50% or more, preferably 80% or more, and more preferably 100% or more. Furthermore, the binding activity may be increased by amino acid alterations. For example, the binding activity may be 2-, 5-, 10-times higher or such than that before alteration. In the antibodies of the present invention, alteration of amino acid sequence may be at least one of amino acid residue substitution, addition, deletion, and modification.

[0302] For example, the modification of the N-terminal glutamine of a variable region into pyroglutamic acid by pyroglutamylation is a modification well known to those skilled in the art. Thus, when the heavy-chain N terminus is glutamine, the antibodies of the present invention comprise the variable regions in which the glutamine is modified to pyroglutamic acid.

[0303] Antibody variable regions of the present invention may have any sequences, and they may be antibody variable regions of any origin, such as mouse antibodies, rat antibodies, rabbit antibodies, goat antibodies, camel antibodies, humanized antibodies produced by humanizing these non-human antibodies, and human antibodies. “Humanized antibodies”, also referred to as “reshaped human antibodies”, are antibodies in which the complementarity determining regions (CDRs) of an antibody derived from a non-human mammal, for example, a mouse antibody, are transplanted into the CDRs of a human antibody. Methods for identifying CDRs are known (Kabat et al., Sequence of Proteins of Immunological Interest (1987), National Institute of Health, Bethesda, Md.; Chothia et al., Nature (1989) 342: 877). Their common genetic recombination techniques are also known (see, European Patent Application Publication No. EP 125023 and WO 96/02576). Furthermore, these antibodies may have various amino acid substitutions introduced into their variable regions to improve their antigen binding, pharmacokinetics, stability, and antigenicity. Variable regions of the antibodies of the present invention may be able to bind antigens repeatedly due to their pH dependability in antigen binding (WO 2009/125825).

[0304] κ chain and λ chain-type constant regions are present in antibody light-chain constant regions, but either one of the light chain constant regions is acceptable. Furthermore, light-chain constant regions of the present invention may be light-chain constant regions with amino acid alterations such as substitutions, deletions, additions, and/or insertions.

[0305] For example, for the heavy chain constant regions of an antibody of the present invention, heavy chain constant regions of human IgG antibodies may be used and heavy chain constant regions of human IgG1 antibodies and those of human IgG4 antibodies are preferred.

[0306] Furthermore, Fc region variants of the present invention may be made into Fc fusion protein molecules by linking to other proteins, physiologically active peptides, and such. Herein, fusion protein refers to a chimeric polypeptide comprising at least two different polypeptides, which do not spontaneously link with each other in natural. Examples of the other proteins and biologically active peptides include receptors, adhesion molecules, ligands, and enzymes, but are not limited thereto.

[0307] Preferred examples of Fc fusion protein molecules of the present invention include proteins with Fc region fused to a receptor protein that binds to a target, and such examples include TNFR-Fc fusion protein, IL1R-Fc fusion protein, VEGFR-Fc fusion protein, and CTLA4-Fc fusion protein (Nat Med. 2003 January; 9(1): 47-52; BioDrugs. 2006; 20(3): 151-60). Furthermore, a protein to be fused to a polypeptide of the present invention may be any molecule as long as it binds to a target molecule, and examples include scFv molecules (WO 2005/037989), single-domain antibody molecules (WO 2004/058821; WO 2003/002609), antibody-like molecules (Current Opinion in Biotechnology 2006, 17: 653-658; Current Opinion in Biotechnology 2007, 18: 1-10; Current Opinion in Structural Biology 1997, 7: 463-469; and Protein Science 2006, 15: 14-27) such as DARPins (WO 2002/020565), Affibody (WO 1995/001937), Avimer (WO 2004/044011; WO 2005/040229), and Adnectin (WO 2002/032925). Furthermore, antibodies and Fc fusion protein molecules may be multispecific antibodies that bind to multiple types of target molecules or epitopes.

[0308] Furthermore, the antibodies of the present invention include antibody modification products. Such antibody modification products include, for example, antibodies linked with various molecules such as polyethylene glycol (PEG) and cytotoxic substances. Such antibody modification products can be obtained by chemically modifying antibodies of the present invention. Methods for modifying antibodies are already established in this field.

[0309] The antibodies of the present invention may also be bispecific antibodies. Bispecific antibody refers to an antibody that has in a single molecule variable regions that recognize different epitopes. The epitopes may be present in a single molecule or in different molecules.

[0310] The polypeptides of the present invention can be prepared by the methods known to those skilled in the art. For example, the antibodies can be prepared by the methods described below, but the methods are not limited thereto.

[0311] A DNA encoding an antibody heavy chain in which one or more amino acid residues in the Fc region have been substituted with other amino acids of interest and DNA encoding an antibody light chain, are expressed. A DNA encoding a heavy chain in which one or more amino acid residues in the Fc region are substituted with other amino acids of interest can be prepared, for example, by obtaining a DNA encoding the Fc region of a natural heavy chain, and introducing an appropriate substitution so that a codon encoding a particular amino acid in the Fc region encodes another amino acid of interest.

[0312] Alternatively, a DNA encoding a heavy chain in which one or more amino acid residues in the Fc region are substituted with other amino acids of interest can also be prepared by designing and then chemically synthesizing a DNA encoding a protein in which one or more amino acid residues in the Fc region of the natural heavy chain are substituted with other amino acids of interest. The position and type of amino acid substitution are not particularly limited. Furthermore, alteration is not limited to substitution, and alteration may be any of deletion, addition, or insertion, or combination thereof

[0313] Alternatively, a DNA encoding a heavy chain in which one or more amino acid residues in the Fc region are substituted with other amino acids of interest can be prepared as a combination of partial DNAs. Such combinations of partial DNAs include, for example, the combination of a DNA encoding a variable region and a DNA encoding a constant region, and the combination of a DNA encoding an Fab region and a DNA encoding an Fc region, but are not limited thereto. Furthermore, a DNA encoding a light chain can similarly be prepared as a combination of partial DNAs.

[0314] Methods for expressing the above-described DNAs include the methods described below. For example, a heavy chain expression vector is constructed by inserting a DNA encoding a heavy chain variable region into an expression vector along with a DNA encoding a heavy chain constant region. Likewise, a light chain expression vector is constructed by inserting a DNA encoding a light chain variable region into an expression vector along with a DNA encoding a light chain constant region. Alternatively, these heavy and light chain genes may be inserted into a single vector.

[0315] When inserting a DNA encoding the antibody of interest into an expression vector, the DNA is inserted so that the antibody is expressed under the control of an expression-regulating region such as an enhancer or promoter. Next, host cells are transformed with this expression vector to express the antibody. In such cases, an appropriate combination of host and expression vector may be used.

[0316] Examples of the vectors include M13 vectors, pUC vectors, pBR322, pBluescript, and pCR-Script. Alternatively, when aiming to subclone and excise cDNA, in addition to the vectors described above, pGEM-T, pDIRECT, pT7, and such can be used.

[0317] Expression vectors are particularly useful when using vectors for producing the polypeptides of the present invention. For example, when a host cell is E. coli such as JM109, DH5α, HB101, and XL1-Blue, the expression vectors must carry a promoter that allows efficient expression in E. coli, for example, lacZ promoter (Ward et al., Nature (1989) 341: 544-546; FASEB J. (1992) 6: 2422-2427; its entirety are incorporated herein by reference), araB promoter (Better et al., Science (1988) 240: 1041-1043; its entirety are incorporated herein by reference), T7 promoter, or such. Such vectors include pGEX-5X-1 (Pharmacia), “QIAexpress system” (QIAGEN), pEGFP, or pET (in this case, the host is preferably BL21 that expresses T7 RNA polymerase) in addition to the vectors described above.

[0318] The vectors may contain signal sequences for polypeptide secretion. As a signal sequence for polypeptide secretion, a pelB signal sequence (Lei, S. P. et al J. Bacteriol. (1987) 169: 4397; its entirety are incorporated herein by reference) may be used when a polypeptide is secreted into the E. coli periplasm. The vector can be introduced into host cells by lipofectin method, calcium phosphate method, and DEAE-Dextran method, for example.

[0319] In addition to E. coli expression vectors, the vectors for producing the polypeptides of the present invention include mammalian expression vectors (for example, pcDNA3 (Invitrogen), pEGF-BOS (Nucleic Acids. Res. 1990, 18(17): p5322; its entirety are incorporated herein by reference), pEF, and pCDM8), insect cell-derived expression vectors (for example, the “Bac-to-BAC baculovirus expression system” (GIBCO-BRL) and pBacPAK8), plant-derived expression vectors (for example, pMH1 and pMH2), animal virus-derived expression vectors (for example, pHSV, pMV, and pAdexLcw), retroviral expression vectors (for example, pZIPneo), yeast expression vectors (for example, “Pichia Expression Kit” (Invitrogen), pNV11, and SP-Q01), and Bacillus subtilis expression vectors (for example, pPL608 and pKTH50), for example.

[0320] When aiming for expression in animal cells such as CHO, COS, and NIH3T3 cells, the vectors must have a promoter essential for expression in cells, for example, SV40 promoter (Mulligan et al., Nature (1979) 277: 108; its entirety are incorporated herein by reference), MMTV-LTR promoter, EF1α promoter (Mizushima et al., Nucleic Acids Res. (1990) 18: 5322; its entirety are incorporated herein by reference), CAG promoter (Gene. (1990) 18: 5322; its entirety are incorporated herein by reference), and CMV promoter, and more preferably they have a gene for selecting transformed cells (for example, a drug resistance gene that allows evaluation using an agent (neomycin, G418, or such)). Vectors with such characteristics include pMAM, pDR2, pBK-RSV, pBK-CMV, pOPRSV, and pOP13, for example.

[0321] In addition, the following method can be used for stable gene expression and gene copy number amplification in cells: CHO cells deficient in a nucleic acid synthesis pathway are introduced with a vector that carries a DHFR gene which compensates for the deficiency (for example, pCHOI), and the vector is amplified using methotrexate (MTX). Alternatively, the following method can be used for transient gene expression: COS cells with a gene expressing SV40 T antigen on their chromosome are transformed with a vector with an SV40 replication origin (pcD and such). Replication origins derived from polyoma virus, adenovirus, bovine papilloma virus (BPV), and such can also be used. To amplify gene copy number in host cells, the expression vectors may further carry selection markers such as aminoglycoside transferase (APH) gene, thymidine kinase (TK) gene, E. coli xanthine-guanine phosphoribosyltransferase (Ecogpt) gene, and dihydrofolate reductase (dhfr) gene.

[0322] Antibodies can be collected, for example, by culturing transformed cells, and then separating the antibodies from the inside of the transformed cells or from the culture media. Antibodies can be separated and purified using an appropriate combination of methods such as centrifugation, ammonium sulfate fractionation, salting out, ultrafiltration, 1q, FcRn, protein A, protein G column, affinity chromatography, ion exchange chromatography, and gel filtration chromatography.

[0323] Furthermore, the present invention provides methods of promoting elimination of a disease-causing antigen in plasma by using a polypeptide comprising an Fc region variant of the present invention and an antigen-binding domain having a binding activity to the antigen and whose binding activity to the antigen changes depending on the ion concentration condition, wherein the antigen exists in a soluble form in plasma.

[0324] As described in WO2011/122011, a pH-dependent antigen-binding molecule produced by further modifying a pH-dependent antigen-binding molecule to enhance its FcRn binding under a neutral condition (pH 7.4) has been reported to be able to eliminate antigens from plasma by administration of a polypeptide comprising such an antigen-binding domain, since the molecule has an effect of enabling repeated antigen binding and an effect of eliminating antigens from plasma (WO2011/122011). However, so far there are no reports on methods for accelerating antigen elimination besides the method of enhancing FcRn binding under neutral conditions.

[0325] In the Examples, a polypeptide comprising an antigen-binding domains whose antigen-binding activity changes depending on the pH condition was found to show accelerated elimination of antigens in plasma through FcγR binding as compared to when only the antigen exists, even though the polypeptide comprises a native IgG1-derived Fc region whose binding to FcRn in the neutral pH range is not enhanced. Without being restricted to a particular theory, the following mechanism is an example of the reason why such phenomena occurs in clone 278 and such.

[0326] When the site to which the antigen-binding domain can bind is one (that is, a homo-monomer) such as in sIL-6R, two molecules of antigens bind to one antibody molecule comprising divalent antigen-binding domains, and one anti-sIL-6R antibody molecule and two antigen molecules comprising two antigen-binding units form a complex. Therefore, this type of antigen-antibody complex has only one Fc region (native IgG1 Fc region) as shown in FIG. 9. Since the complex binds to two molecules of FcRn or one molecule of FcγR via a single Fc region, affinities for these receptors are the same as those of normal IgG antibodies, and uptake into cells can be considered to take place mostly non-specifically.

[0327] On the other hand, when epitopes to which the antigen-binding domain binds are present at two sites in an antigen such as when an antigen is a heterodimeric complex of heavy and light chains, e.g., human IgE, binding of each of the bivalent antigen-binding domains in a single anti-IgE antibody molecule to each of the two units of epitopes in a single IgE molecule may be difficult in terms of epitope placement and such. As a result, it is considered that an antigen-antibody complex (immune complex) comprising at least four molecules (that is, two molecules of IgE, which are antigen molecules, and two molecules of anti-IgE antibodies, which are polypeptides comprising the antigen-binding domain) is formed by the binding of a separate anti-IgE antibody molecule to two antigen-binding units present in two IgE molecules that bind to the bivalent antigen-binding domain present in a single anti-IgE antibody molecule.

[0328] When a polypeptide comprising an antigen-binding domain such as an antibody that binds to an antigen molecule comprising two or more sites to which an antigen-binding domain can bind forms a large immune complex which is at least a tetramer, such immune complex can bind strongly with avidity via at least two or more multivalent Fc regions to FcγR, FcRn, complement receptor, and such. On the other hand, when an antigen molecule has one site to which the antigen-binding domain can bind, the Fc region-mediated affinity of immune complexes formed between the antigen molecule and the polypeptide comprising an antigen-binding domain for these receptors is insufficient compared to when the above-mentioned immune complexes are formed. More specifically, the immune complex is incorporated highly efficiently by cells expressing these receptors.

[0329] When an antigen molecule comprises two or more sites that bind to an antigen-binding domain, a polypeptide of the present invention, in case when the polypeptide is, for example, an antibody, forms antigen-antibody complexes (immune complexes) comprising at least four molecules (two antigen molecules and two antibody molecules) in plasma, and when the immune complexes are incorporated into cells, antigens dissociate from the antibodies in the endosome where the ion concentration condition is different from that in plasma as the polypeptide of the present invention has an antigen-binding domain whose antigen binding varies depending on the ion concentration condition such as pH-dependent binding. Therefore, the formed immune complexes are eliminated in the endosome of cells into which the immune complexes were incorporated. Since the dissociated antigens cannot bind to FcRn in the endosome, they are degraded after being transferred to the lysosome. On the other hand, antibodies that dissociate from the antigen may be recycled into the plasma after binding to FcRn in the endosome. Similar recycling is possible using an ion concentration condition other than the pH condition as described in the Examples, and Reference Examples 3 to 6 show that elimination of antigens in plasma can be accelerated using an antigen-binding domain whose antigen-binding activity changes depending on the calcium concentration condition instead of the pH-dependent condition.

[0330] Therefore, when an immune complex formed by antigens and polypeptides comprising an antigen-binding domain for the antigen is a complex comprising two or more multivalent Fc regions for FcγR, FcRn, complement receptor, and such, the complex can accelerate elimination of the antigens.

[0331] In addition, the examinations of Reference Examples 7 to 9 show that among the FcγRs, FcγRIIB which is an inhibitory FcγR makes the largest contribution to elimination of antigens via FcγR. More specifically, even if FcγR binding is decreased, the ability of an antibody to eliminate antigens via FcγR can be maintained as long as FcγRIIB binding can be maintained.

[0332] To date, side effects due to interactions between IgG and FcγR have been reported for several therapeutic antibodies. For example, in the group of patients who were administered with bevacizumab, an antibody against VEGF, frequency for developing thromboembolism is known to increase (J. Natl. Cancer Inst. (2007) 99 (16), 1232-1239). Furthermore, thromboembolism has been observed similarly in clinical development tests of antibodies against the CD40 ligand, and the clinical trial was discontinued (Arthritis. Rheum. (2003) 48 (3), 719-727). FcγRIIa, an activating Fcγ receptor, is expressed on platelet cells, while FcγRIIb, an inhibitory Fcγ receptor, is not expressed (J. Exp. Med. (2006) 203 (9), 2157-2164); and later studies using animal models and such have suggested that both of the administered antibodies aggregate platelets via binding to FcγRIIa on the platelets, resulting in the formation of blood clots (J. Thromb. Haemost. (2009) 7 (1), 171-181; and J. Immunol. (2010) 185 (3), 1577-1583). In patients with systemic lupus erythematosus which is an autoimmune disease, platelets are activated through an FcγRIIa-dependent mechanism, and platelet activation has been reported to correlate with the severity of symptoms (Sci. Transl. Med. (2010) 2 (47), 47-63).

[0333] Furthermore, it has been reported to date that through studies using animal models, an immune complex formed between an antibody and a multivalent antigen induced anaphylaxis via activating FcγRs (Bruhns P., Blood. (2012) 119(24): 5640-9).

[0334] Uptake of immune complexes formed between a multivalent antigen and an antibody via activating FcγR has been reported to increase antibody titer production for the antigen (Scand J Immunol. (2006) 64(3): 177-84.; J Immunol. (1999) 163: 618-22). This suggests the possibility that antibodies against the therapeutic antibody itself are likely to be produced for therapeutic antibodies that recognize multivalent antigens. When antibodies against a therapeutic antibody are produced, kinetics in blood worsens and effects may be weakened.

[0335] This way, by binding of antibodies with multivalent antigens to form immune complexes, these immune complexes may induce various side effects by interacting with activating FcγR, and lowers the value of the antibody as pharmaceutical. Examples of multivalent antigens (multimeric antigens) include GDF, GDF-1, GDF-3 (Vgr-2), GDF-5 (BMP-14, CDMP-1), GDF-6 (BMP-13, CDMP-2), GDF-7 (BMP-12, CDMP-3), GDF-8 (myostatin), GDF-9, GDF-15 (MIC-1), TNF, TNF-alpha, TNF-alpha/beta, TNF-beta 2, TNFSF10 (TRAIL Apo-2 ligand, TL2), TNFSF11 (TRANCE/RANK ligand ODF, OPG ligand), TNFSF12 (TWEAK Apo-3 ligand, DR3 ligand), TNFSF13 (APRIL TALL2), TNFSF13B (BAFF BLYS, TALL1, THANK, TNFSF20), TNFSF14 (LIGHT HVEM ligand, LTg), TNFSF15 (TL1A/VEGI), TNFSF18 (GITR ligand AITR ligand, TL6), TNFSF1A (TNF-α Conectin, DIF, TNFSF2), TNFSF1B (TNF-b LTa, TNFSF1), TNFSF3 (LTb TNFC, p33), TNFSF4 (OX40 ligand gp34, TXGP1), TNFSF5 (CD40 ligand CD154, gp39, HIGM1, IMD3, TRAP), TNFSF6 (Fas ligand Apo-1 ligand, APT1 ligand), TNFSF7 (CD27 ligand CD70), TNFSF8 (CD30 ligand CD153), TNFSF9 (4-1BB ligand CD137 ligand), VEGF, IgE, IgA, IgG, IgM, RANKL, TGF-alpha, TGF-beta, TGF-beta Pan Specific, and IL-8.

[0336] Methods for solving these issues may be methods of attenuating FcγR binding. However, if binding to all FcγRs is attenuated, acceleration of antigen elimination via FcγRs may not be possible using that antibody.

[0337] As described earlier, since from among the FcγRs, FcγRIIB plays a main role in FcγR-mediated antigen elimination by antibodies and side effects due to FcγR interactions are caused by interactions with activating FcγRs, it is possible to produce excellent antibodies with decreased side effects by activating FcγRs without losing the ability to eliminate antigens by selectively attenuating binding to other activating FcγRs while maintaining FcγRIIB binding.

[0338] Therefore, an excellent effect of promoting elimination of disease-causing soluble antigens from the plasma can be achieved by polypeptides comprising an Fc region variant of the present invention and an antigen-binding domain whose binding activity to the antigen changes according to the ion concentration condition.

[0339] Furthermore, by using the polypeptides of the present invention, similar effects can be achieved even if antigens to which the polypeptides bind have only one site that can bind to an antigen-binding domain (monomeric antigen).

[0340] Such an example includes the method of promoting elimination of monomeric antigens from plasma using a cocktail of polypeptides comprising antigen-binding domains.

[0341] As described above, when an antigen is a multimeric antigen (a non-limiting example is an immunoglobulin such as IgA or IgE, or a member of the TNF superfamily such as TNF or CD154), a large immune complex comprising two or more antigen-binding molecules and two or more antigen-binding units may be formed. On the other hand, even when an antigen is a monomeric antigen, a mixture of polypeptides comprising two or more appropriate antigen-binding domains that individually bind to different epitopes present in the monomeric antigen, where binding to the epitopes varies depending on the ion concentration condition (such as pH or Ca), may also be able to form large immune complexes comprising a polypeptide having two or more antigen-binding domains, and two or more binding sites for antigen-binding domains (monomeric antigens). Herein, a mixture of polypeptides comprising two or more appropriate antigen-binding domains that individually bind to different epitopes present in the monomeric antigen, where binding of the antigen-binding molecules to the epitopes varies depending on the ion concentration condition (such as pH or Ca) is called an antigen-binding-molecule cocktail. Among these antigen-binding domain-comprising polypeptides, at least one of the (antigen-binding domains contained in) the polypeptides forming the immune complex has to be an antigen-binding domain whose antigen-binding activity varies according to the ion concentration condition.

[0342] Other examples include methods of promoting elimination of monomeric antigens from plasma using a polypeptide comprising multispecific or multiparatopic antigen-binding domains.

[0343] Alternatively, even when the antigen is a monomeric antigen, each antigen-binding domain contained in a polypeptide comprising antigen-binding domains that individually bind to different epitopes present in the monomeric antigen, and antigen-binding molecules comprising antigen-binding domains in which the epitope binding of the individual antigen-binding domains varies according to the ion concentration condition (such as pH or Ca), may also be able to form a large immune complex comprising a polypeptide containing two or more antigen-binding domains and two or more antigen binding units (monomeric antigens). Examples of a non-limiting embodiment of such a polypeptide include multispecific or multiparatopic antibodies comprising appropriate variable regions that individually bind to different epitopes present on a monomeric antigen. As a non-limiting embodiment of such multispecific antibodies or multiparatopic antibodies, antibodies whose variable regions show pH- or Ca-dependent binding (bispecific antibodies or biparatopic antibodies comprising a right-arm variable region which recognizes epitope A and a left-arm variable region that recognizes epitope B, as shown in FIG. 19) may also be able to form large immune complexes comprising two or more antibodies and two or more antigen-binding units (monomeric antigens).

[0344] An antigen-binding molecule that further accelerates elimination of monomeric antigens from plasma can be obtained by screening for combinations of antigen-binding domains targeting different epitopes of a monomeric antigen, where the activity to bind to each of the epitopes varies depending on the ion concentration condition, and the antigen-binding domains can bind with avidity to the above-mentioned receptors. The ion concentration condition that changes the binding activity of the multispecific or multiparatopic antigen-binding domain to each of the epitopes may be the same ion concentration condition or a different ion concentration condition. For example, an antigen-binding molecule comprising bispecific or biparatopic antigen-binding domains, where the epitope-binding activity of one of the antigen-binding domains varies depending on the pH condition or metal ion concentration condition such as Ca ion concentration, may be exemplified as a non-limiting embodiment of the antigen-binding molecule of the present invention. Furthermore, for example, an antigen-binding molecule comprising bispecific or biparatopic antigen-binding domains, where the epitope-binding activity of one of the antigen-binding domains varies depending on the pH condition and the epitope-binding activity of the other antigen-binding domain varies depending on the condition of metal ion concentration such as Ca ion concentration, may also be exemplified as a non-limiting embodiment of the antigen-binding molecule of the present invention. An antigen-binding molecule comprising bispecific or biparatopic antigen-binding domains, where the epitope-binding activity of one of the antigen-binding domains varies depending on the pH condition and the epitope-binding activity of the other antigen-binding domain also varies depending on the pH condition, may also be exemplified as a non-limiting embodiment of the antigen-binding molecule of the present invention. A polypeptide antigen-binding molecule comprising bispecific or biparatopic antigen-binding domains, where the epitope-binding activity of one of the antigen-binding domains varies depending on the condition of metal ion concentration such as Ca ion concentration, and the epitope-binding activity of the other antigen-binding domain also varies depending on the condition of metal ion concentration such as Ca ion concentration, may also be exemplified as a non-limiting embodiment of the antigen-binding molecule of the present invention.

[0345] As for a polypeptide comprising multispecific antigen-binding domains or a polypeptide molecule comprising multiparatopic antigen-binding domains of the present invention, a polypeptide comprising at least two antigen-binding domains, wherein at least one of the antigen-binding domains binds to a first epitope in an antigen molecule, and at least another one of the antigen-binding domains binds to a second epitope in the antigen molecule, is called a multispecific antigen-binding molecule from the viewpoint of its reaction specificity. When a single antigen-binding molecule binds to two different epitopes through two types of antigen-binding domains comprised in the antigen-binding molecule, this molecule is called a bispecific antigen-binding molecule. When a single antigen-binding molecule binds to three different epitopes through three types of antigen-binding domains comprised in the antigen-binding molecule, this molecule is called a trispecific antigen-binding molecule.

[0346] A paratope in the antigen-binding domain that binds to the first epitope in the antigen molecule and a paratope in the antigen-binding domain that binds to the second epitope structurally different from the first epitope are paratopes that have different structures. Therefore, an antigen-binding molecule comprising at least two antigen-binding domains, wherein at least one of the antigen-binding domains binds to a first epitope in an antigen molecule, and at least another one of the antigen-binding domains binds to a second epitope in the antigen molecule, is called a multiparatopic antigen-binding molecule from the viewpoint of its structural specificity. When a single antigen-binding molecule binds to two different epitopes through two types of antigen-binding domains comprised in the antigen-binding molecule, this molecule is called a biparatopic antigen-binding molecule. When a single antigen-binding molecule binds to three different epitopes through three types of antigen-binding domains comprised in the antigen-binding molecule, this molecule is called a triparatopic antigen-binding molecule.

[0347] Multivalent multispecific or multiparatopic antigen-binding molecules comprising one or more antigen-binding domains and their preparation methods are described in non-patent documents such as Conrath et al., (J. Biol. Chem. (2001) 276 (10) 7346-7350), Muyldermans (Rev. Mol. Biotech. (2001) 74, 277-302), and Kontermann R. E. (2011) Bispecific Antibodies (Springer-Verlag), and in Patent Documents such as WO1996/034103 and WO1999/023221. Antigen-binding molecules of the present invention can be produced using multispecific or multiparatopic antigen-binding molecules and preparation methods described in these documents.

[0348] Bispecific antibodies and methods of producing them are presented below as examples of an embodiment of the aforementioned multispecific or multiparatopic antigen-binding molecules and their preparation methods. Bispecific antibodies are antibodies comprising two types of variable regions that bind specifically to different epitopes. IgG-type bispecific antibodies can be secreted from a hybrid hybridoma (quadroma) produced by fusing two types of hybridomas that produce IgG antibodies (Milstein et al., Nature (1983) 305, 537-540).

[0349] When a bispecific antibody is produced by using recombination techniques such as those described in the above-mentioned section on antibodies, one may adopt a method that introduces genes encoding heavy chains containing the two types of variable regions of interest into cells to co-express them. However, even when only the heavy-chain combination is considered, such a co-expression method will produce a mixture of (i) a combination of a pair of heavy chains in which one of the heavy chains contains a variable region that binds to a first epitope and the other heavy chain contains a variable region that binds to a second epitope, (ii) a combination of a pair of heavy chains which include only heavy chains containing a variable region that binds to the first epitope, and (iii) a combination of a pair of heavy chains which include only heavy chains containing a variable region that binds to the second epitope, which are present at a molecular ratio of 2:1:1. It is difficult to purify antigen-binding molecules containing the desired combination of heavy chains from the mixture of three types of heavy chain combinations.

[0350] When producing bispecific antibodies using recombination techniques such as described above, bispecific antibodies comprising the hetero combination of heavy chains can be preferentially secreted by altering the CH3 domain that constitutes a heavy chain using appropriate amino acid substitutions. Specifically, it is a method of enhancing heterogeneous heavy chain formation and inhibiting homogeneous heavy chain formation by substituting amino acid side chain in one heavy chain CH3 domain with a bulker side chain (knob (meaning “projection”)) while substituting amino acid side chain in the other heavy chain CH3 domain with a smaller side chain (hole (meaning “void”)) so that the “knob” is placed in the “hole” (WO 1996/027011, Ridgway et al. (Protein Engineering (1996) 9, 617-621), Merchant et al. (Nat. Biotech. (1998) 16, 677-681)).

[0351] Furthermore, known techniques for producing bispecific antibodies include those in which a means for regulating polypeptide association or association to form heteromeric multimers constituted by polypeptides is applied to the association of heavy chains. Specifically, to produce bispecific antibodies, one can use methods for regulating heavy chain association by altering amino acid residues forming interface between heavy chains so as to form two heavy chains with different sequences, while inhibiting the association of heavy chains having an identical sequence (WO 2006/106905). Such methods can be used to produce bispecific antibodies.

[0352] Furthermore, a technique for obtaining bispecific antibodies by individually obtaining two types of monoclonal antibodies, and combining them in vitro in the presence of a reducing agent has been reported (WO2008/119353). In this technique, two types of monoclonal antibodies are cleaved into half molecules by the reducing agent, and by reassembling them, bispecific antibodies are obtained at a certain rate. Furthermore, methods of obtaining bispecific antibodies more efficiently by substituting amino acids present in the CH3 domain to regulate the reassembly of the half molecules have been reported (WO2011/131746). Such methods may also be employed when producing bispecific antibodies.

[0353] In the present invention, “promoting elimination of antigens from plasma” refers to improvement of the ability to eliminate antigens from plasma when a polypeptide comprising the antigen-binding domains (hereinafter also referred to as antigen-binding molecule) is administered in vivo or when an antigen-binding molecule is secreted in vivo. Accordingly, it means that when the antigen-binding molecules are administered, the rate of antigen elimination from plasma is accelerated as compared to when an antigen-binding molecule comprising an antigen-binding domain whose antigen-binding activity does not vary depending on ion concentrations, an antigen-binding molecule comprising an FcRn-binding domain without FcRn-binding activity under an acidic pH range condition, or an antigen-binding molecule comprising an Fcγ receptor-binding domain without selective binding activity to an Fcγ receptor is administered. Whether or not the ability of an antigen-binding molecule to eliminate antigens in the plasma increased can be determined, for example, by administering soluble antigens and the antigen-binding molecule in vivo, and then measuring the plasma concentration of the soluble antigen after administration. When the concentration of the soluble antigens in the plasma is decreased after administration of the soluble antigens and the antigen-binding molecules comprising an antigen-binding domain whose antigen-binding activity varies depending on ion concentration conditions, the FcRn-binding domain having FcRn-binding activity under an acidic pH range condition, and an Fcγ receptor-binding domain having selective binding activity to an Fcγ receptor (a selective FcγR-binding domain), the ability of the antigen-binding molecules to eliminate antigens in the plasma is judged to be increased. Here, selective FcγR-binding domain refers to a domain whose binding to activating FcγRs is decreased while binding to FcγRIIb is maintained. The soluble antigen may be an antigen that is bound to an antigen-binding molecule or an antigen that is not bound to an antigen-binding molecule in the plasma, and its concentration can be determined as a “plasma concentration of the antigen bound to the antigen-binding molecule” or as a “plasma concentration of the antigen not bound to the antigen-binding molecule”, respectively (the latter is synonymous with “free antigen concentration in plasma”). The “total antigen concentration in plasma” means the sum of concentrations of the antigen-binding molecule-bound antigen and the antigen not bound by an antigen-binding molecule, or the “free antigen concentration in plasma” which is the concentration of the antigen not bound by an antigen-binding molecule. Thus, the soluble antigen concentration can be determined as the “total antigen concentration in plasma”. Various methods for measuring the “total antigen concentration in plasma” or the “free antigen concentration in plasma” are well known in the art as described hereinafter.

[0354] In the present invention, “enhancement of pharmacokinetics”, “improvement of pharmacokinetics”, and “superior pharmacokinetics” can be restated as “enhancement of plasma (blood) retention”, “improvement of plasma (blood) retention”, “superior plasma (blood) retention”, and “prolonged plasma (blood) retention”. These terms are synonymous.

[0355] In the present invention, “improvement of pharmacokinetics” means not only prolongation of the period until elimination from the plasma (for example, until the antigen-binding molecule is degraded intracellularly or the like and cannot return to the plasma) after administration of the antigen-binding molecule to humans, or non-human animals such as mice, rats, monkeys, rabbits, and dogs, but also prolongation of the plasma retention of the antigen-binding molecule in a form that allows antigen binding (for example, in an antigen-free form of the antigen-binding molecule) during the period of administration to elimination due to degradation. Human IgG having native Fc region can bind to FcRn from non-human animals. For example, mouse can be preferably used to be administered in order to confirm the property of the antigen-binding molecule of the invention since human IgG having native Fc region can bind to mouse FcRn stronger than to human FcRn (Int Immunol. (2001) 13(12): 1551-1559). As another example, mouse in which its native FcRn genes are disrupted and a transgene for human FcRn gene is harbored to be expressed (Methods Mol Biol. 2010; 602: 93-104) can also be preferably used to be administered in order to confirm the property of the antigen-binding molecule of the invention described hereinafter. Specifically, “improvement of pharmacokinetics” also includes prolongation of the period until elimination due to degradation of the antigen-binding molecule not bound to antigens (the antigen-free form of antigen-binding molecule). The antigen-binding molecule in plasma cannot bind to a new antigen if the antigen-binding molecule has already bound to an antigen. Thus, the longer the period that the antigen-binding molecule is not bound to an antigen, the longer the period that it can bind to a new antigen (the higher the chance of binding to another antigen). This enables reduction of the time period that an antigen is free of the antigen-binding molecule in vivo and prolongation of the period that an antigen is bound to the antigen-binding molecule. The plasma concentration of the antigen-free form of antigen-binding molecule can be increased and the period that the antigen is bound to the antigen-binding molecule can be prolonged by accelerating the antigen elimination from the plasma by administration of the antigen-binding molecule. Specifically, “improvement of the pharmacokinetics of antigen-binding molecule” in the present invention includes the improvement of a pharmacokinetic parameter of the antigen-free form of the antigen-binding molecule (any of prolongation of the half-life in plasma, prolongation of mean retention time in plasma, and impairment of plasma clearance), prolongation of the period that the antigen is bound to the antigen-binding molecule after administration of the antigen-binding molecule, and acceleration of antigen-binding molecule-mediated antigen elimination from the plasma. The improvement of pharmacokinetics of antigen-binding molecule can be assessed by determining any one of the parameters, half-life in plasma, mean plasma retention time, and plasma clearance for the antigen-binding molecule or the antigen-free form thereof (“Pharmacokinetics: Enshu-niyoru Rikai (Understanding through practice)” Nanzando). For example, the plasma concentration of the antigen-binding molecule or antigen-free form thereof is determined after administration of the antigen-binding molecule to mice, rats, monkeys, rabbits, dogs, or humans. Then, each parameter is determined. When the plasma half-life or mean plasma retention time is prolonged, the pharmacokinetics of the antigen-binding molecule can be judged to be improved. The parameters can be determined by methods known to those skilled in the art. The parameters can be appropriately assessed, for example, by noncompartmental analysis using the pharmacokinetics analysis software WinNonlin (Pharsight) according to the appended instruction manual. The plasma concentration of antigen-free antigen-binding molecule can be determined by methods known to those skilled in the art, for example, using the assay method described in Clin Pharmacol. 2008 April; 48 (4): 406-417.

[0356] In the present invention, “improvement of pharmacokinetics” also includes prolongation of the period that an antigen is bound to an antigen-binding molecule after administration of the antigen-binding molecule. Whether the period that an antigen is bound to the antigen-binding molecule after administration of the antigen-binding molecule is prolonged can be assessed by determining the plasma concentration of free antigen. The prolongation can be judged based on the determined plasma concentration of free antigen or the time period required for an increase in the ratio of free antigen concentration to the total antigen concentration.

[0357] The plasma concentration of free antigen not bound to the antigen-binding molecule or the ratio of free antigen concentration to the total concentration can be determined by methods known to those skilled in the art, for example, by the method used in Pharm Res. 2006 January; 23 (1): 95-103. Alternatively, when an antigen exhibits a particular function in vivo, whether the antigen is bound to an antigen-binding molecule that neutralizes the antigen function (antagonistic molecule) can be assessed by testing whether the antigen function is neutralized. Whether the antigen function is neutralized can be assessed by assaying an in vivo marker that reflects the antigen function. Whether the antigen is bound to an antigen-binding molecule that activates the antigen function (agonistic molecule) can be assessed by assaying an in vivo marker that reflects the antigen function.

[0358] Determination of the plasma concentration of free antigen and ratio of the amount of free antigen in plasma to the amount of total antigen in plasma, in vivo marker assay, and such measurements are not particularly limited; however, the assays are preferably carried out after a certain period of time has passed after administration of the antigen-binding molecule. In the present invention, the period after administration of the antigen-binding molecule is not particularly limited; those skilled in the art can determine the appropriate period depending on the properties and the like of the administered antigen-binding molecule. Such periods include, for example, one day after administration of the antigen-binding molecule, three days after administration of the antigen-binding molecule, seven days after administration of the antigen-binding molecule, 14 days after administration of the antigen-binding molecule, and 28 days after administration of the antigen-binding molecule. In the present invention, the concept “plasma antigen concentration” comprises both “total antigen concentration in plasma” which is the sum of antigen-binding molecule bound antigen and non-bound antigen concentration or “free antigen concentration in plasma” which is antigen-binding molecule non-bound antigen concentration.

[0359] The total antigen concentration in the plasma can be lowered by administration, as antigen-binding molecule, of the antigen-binding molecule of the present invention by 2-fold, 5-fold, 10-fold, 20-fold, 50-fold, 100-fold, 200-fold, 500-fold, 1,000-fold, or even higher as compared to administration of an antigen-binding molecule containing an antigen-binding domain whose antigen-binding activity is ion concentration-independent or an antigen-binding molecule containing an Fc region with an impaired FcγR-binding activity, or compared to when the antigen-binding domain molecule of the present invention is not administered.

[0360] Molar antigen/antigen-binding molecule ratio can be calculated as shown below:

value A: Molar antigen concentration at each time point
value B: Molar antigen-binding molecule concentration at each time point
value C: Molar antigen concentration per molar antigen-binding molecule concentration (molar antigen/antigen-binding molecule ratio) at each time point

C=A/B.

[0361] Smaller value C indicates higher efficiency of antigen elimination per antigen-binding molecule whereas higher value C indicates lower efficiency of antigen elimination per antigen-binding molecule.

[0362] Molar antigen/antigen-binding molecule ratio can be calculated as described above.

[0363] A 2-fold, 5-fold, 10-fold, 20-fold, 50-fold, 100-fold, 200-fold, 500-fold, 1,000-fold or even greater reduction of molar antigen/antigen-binding molecule ratio can be achieved by administration of an antigen-binding molecule of the present invention as compared to when an antigen-binding molecule comprising an antigen-binding domain whose antigen-binding activity does not vary depending on ion concentrations, an antigen-binding molecule comprising an FcRn-binding domain without FcRn-binding activity under an acidic pH range condition, or an antigen-binding molecule comprising an Fcγ receptor-binding domain without selective binding activity to an Fcγ receptor is administered as the antigen-binding molecule.

[0364] In the present invention, an antigen-binding molecule comprising an antigen-binding domain whose antigen-binding activity does not vary depending on ion concentrations, an antigen-binding molecule comprising an FcRn-binding domain without FcRn-binding activity under an acidic pH range condition, or an antigen-binding molecule comprising an Fcγ receptor-binding domain without selective binding activity to an Fcγ receptor is used as a reference antigen-binding molecule to be compared with the antigen-binding molecules of the present invention.

[0365] When evaluating the effect of an FcRn-binding domain having FcRn-binding activity under an acidic pH range condition, reduction of total antigen concentration in plasma or molar antigen/antibody ratio can be assessed by either antigen and antibody co-injection model or steady-state antigen infusion model using human FcRn transgenic mouse line 32 or line 276 (Jackson Laboratories, Methods Mol. Biol. (2010) 602, 93-104), when the antigen-binding molecule does not cross-react with the mouse counterpart antigen. When an antigen-binding molecule cross-react with mouse counterpart, they can be assessed by simply injecting antigen-binding molecule to human FcRn transgenic mouse line 32 or line 276 (Jackson Laboratories). In co-injection model, mixture of antigen-binding molecule and antigen is administered to the mouse. In steady-state antigen infusion model, infusion pump containing antigen solution is implanted to the mouse to achieve constant plasma antigen concentration, and then antigen-binding molecule is injected to the mouse. Test antigen-binding molecule is administered at same dosage. Total antigen concentration in plasma, free antigen concentration in plasma and plasma antigen-binding molecule concentration is measured at appropriate time point using method known to those skilled in the art.

[0366] For assessing the effects of an Fcγ receptor-binding domain having selective binding activity to Fcγ receptors, when an antigen-binding molecule does not cross-react with a mouse counterpart antigen, total antigen concentration in plasma or decrease in antigen/antibody mole ratio can be assessed by either the antigen-antibody simultaneous injection model or the steady-state antigen injection model using the conventionally used C57BL/6J mice (Charles River Japan). When an antigen-binding molecule cross-reacts with the mouse counterpart, the antigen-binding molecule can simply be injected to conventionally used C57BL/6J mice (Charles River Japan) to carry out the assessment.

[0367] In the co-injection model, a mixture of the antigen-binding molecule and antigen is administered to mice. In the steady-state antigen infusion model, an infusion pump filled with an antigen solution is embedded into mice to achieve a constant plasma antigen concentration, and then the antigen-binding molecule is injected into the mice. Test antigen-binding molecules are administered at the same dose. The total antigen concentration in plasma, free antigen concentration in plasma, and antigen-binding molecule concentration in plasma are measured at appropriate time points using methods known to those skilled in the art.

[0368] Total or free antigen concentration in plasma and molar antigen/antigen-binding molecule ratio can be measured at 2 days, 4 days, 7 days, 14 days, 28 days, 56 days, or 84 days after administration to evaluate the long-term effect of the present invention. In other words, a long term plasma antigen concentration is determined by measuring total or free antigen concentration in plasma and molar antigen/antigen-binding molecule ratio at 2 days, 4 days, 7 days, 14 days, 28 days, 56 days, or 84 days after administration of an antigen-binding molecule in order to evaluate the property of the antigen-binding molecule of the present invention. Whether the reduction of plasma antigen concentration or molar antigen/antigen-binding molecule ratio is achieved by antigen-binding molecule described in the present invention can be determined by the evaluation of the reduction at any one or more of the time points described above.

[0369] Total or free antigen concentration in plasma and molar antigen/antigen-binding molecule ratio can be measured at 15 minutes, 1 hour, 2 hours, 4 hours, 8 hours, 12 hours, or 24 hours after administration to evaluate the short-term effect of the present invention. In other words, a short term plasma antigen concentration is determined by measuring total or free antigen concentration in plasma and molar antigen/antigen-binding molecule ratio at 15 minutes, 1 hour, 2 hours, 4 hours, 8 hours, 12 hours, or 24 hours after administration of an antigen-binding molecule in order to evaluate the property of the antigen-binding molecule of the present invention.

[0370] Route of administration of an antigen-binding molecule of the present invention can be selected from intradermal, intravenous, intravitreal, subcutaneous, intraperitoneal, parenteral and intramuscular injection.

[0371] In the present invention, improvement of pharmacokinetics of antigen-binding molecule in human is preferred. When the plasma retention in human is difficult to determine, it may be predicted based on the plasma retention in mice (for example, normal mice, human antigen-expressing transgenic mice, human FcRn-expressing transgenic mice) or monkeys (for example, cynomolgus monkeys).

[0372] “The improvement of the pharmacokinetics and prolonged plasma retention of an antigen-binding molecule” in the present invention means improvement of any pharmacokinetic parameter (any of prolongation of the half-life in plasma, prolongation of mean retention time in plasma, reduction of plasma clearance, and bioavailability) after in vivo administration of the antigen-binding molecule, or an increase in the concentration of the antigen-binding molecule in the plasma in an appropriate time after administration. It may be determined by measuring any parameter such as half-life in plasma, mean retention time in plasma, plasma clearance, and bioavailability of the antigen-binding molecule (Pharmacokinetics: Enshu-niyoru Rikai (Understanding through practice), (Nanzando)). For example, when an antigen-binding molecule is administered to mice (normal mice and human FcRn transgenic mice), rats, monkeys, rabbits, dogs, humans, and so on, and the concentration of the antigen-binding molecule in the plasma is determined and each of the parameters is calculated, the pharmacokinetics of the antigen-binding molecule can be judged to be improved when the plasma half-life or mean retention time in the plasma is prolonged. These parameters can be determined by methods known to those skilled in the art. For example, the parameters can be appropriately assessed by non-compartmental analysis using pharmacokinetics analysis software WinNonlin (Pharsight) according to the attached instruction manual.

[0373] Four types of FcγRs, FcγRI, FcγRIIb, FcγRIII, and FcγRIV, have been identified in mice. In humans as well, as corresponding FcγRs, FcγRI, FcγRIIa, FcγRIIb, FcγRIIIa, FcγRIIIa, and FcγRIIIb have been identified. FcγRIIb which is considered to be the only inhibitory type among these FcγRs is conserved in both humans and mice. The other FcγRs, except for FcγRIIIb, transmit activation signals via the immunoreceptor tyrosine-based activating motif (ITAM), whereas FcγRIIb transmits inhibitory signals via the immunoreceptor tyrosine-based inhibitory motif (ITIM) present inside the cells (Nat. Rev. Immunol. (2008) 8, 34-47).

[0374] FcγRIIb1 and FcγRIIb2 have been reported as splicing variants of FcγRIIb. In both humans and mice, FcγRIIb1 has a longer intracellular domain than FcγRIIb2. FcγRIIb1 has been confirmed to be expressed in B cells, and FcγRIIb2 has been confirmed to be expressed in macrophages, mast cells, dendritic cells, basophils, neutrophils, and eosinophils (J. Clin. Immunol. (2005) 25 (1), 1-18).

[0375] So far, in humans, dysfunction and decreased expression of FcγRIIb have been reported to be correlated with onset of autoimmune diseases. For example, it has been reported that in some SLE patients, binding of transcriptional activators is attenuated due to polymorphism in an expression promoter region of FcγRIIb, which results in the decreased FcγRIIb expression (Hum. Genet. (2005) 117, 220-227; J. Immunol. (2004) 172, 7192-7199; and J. Immunol. (2004) 172, 7186-7191). Furthermore, among SLE patients, two types of allotypes have been reported, where the amino acid at position 233 is Ile or Thr in FcγRIIb. This position exists in the transmembrane region of FcγRIIb, and it is reported that FcγRIIb is less likely to exist in the lipid raft when the amino acid at position 233 is Thr compared to when this amino acid is Ile, and as a result, signal transduction function of FcγRIIb decreases (Nat. Med. (2005) 11, 1056-1058; Hum. Mol. Genet., (2005) 14, 2881-2892). In mice as well, knockout mice produced by disrupting the FcγRIIb gene in C57BL/6 mice has been reported to present SLE-like symptoms such as autoantibody production and glomerulonephritis (Immunity 13 (2000) 277-285; J. Exp. Med. (2002) 195, 1167-1174). Furthermore, so far, reduced expression level of FcγRIIb and such have been reported in mice considered to be models with natural onset of SLE (Immunogenetics (2000) 51, 429-435; Int. Immunol. (1999) 11, 1685-1691; Curr. Biol. (2000) 10, 227-230; J. Immunol. (2002) 169, 4340-4346). From these reports, FcγRIIb is considered to regulate humoral immunity in mice as in humans.

[0376] When an antibody carrying an Fc of the present invention eliminates antigens via FcγRIIb, the endocytosis function of FcγRIIb is considered to be making the most important contribution among the functions of FcγRIIb. As described above, FcγRIIb1 and FcγRIIb2 exist as splicing variants of FcγRIIb, but it is reported that the latter is mainly involved in the endocytosis of an immune complex of an antibody and antigen (J. Immunol. (1994), 152 574-585; Science (1992) 256, 1808-1812; Cell (1989) 58, 317-327). So far, mouse FcγRIIb2 has been reported to be incorporated into a clathrin-coated pit and endocytosed (Cell (1989) 58, 317-327). Furthermore, it has been reported that a dileucine motif is necessary for FcγRIIb2-mediated endocytosis, and the dileucine motif is conserved in both humans and mice (EMBO J. (1994) 13 (13), 2963-2969). From these, FcγRIIb2 may have an endocytotic ability in humans as in mice.

[0377] On the other hand, unlike FcγRIIb2, it has been reported that FcγRIIb1 does not cause endocytosis. FcγRIIb1 has an inserted sequence in its intracellular domain that is not found in FcγRIIb2. It is considered that this sequence inhibits the uptake of FcγRIIb1 into a clathrin-coated pit, and as a result endocytosis is inhibited (J. Cell. Biol. (1992) 116, 875-888; J. Cell. Biol. (1989) 109, 3291-3302). In humans as well, FcγRIIb1 has an insertion sequence at a site similar to that of FcγRIIb2 as in mice; therefore, difference in the endocytotic ability between FcγRIIb1 and FcγRIIb2 is presumed to be caused by a similar mechanism. Furthermore, in both humans and mice, approximately 40% of immune complexes on the cell surface is reported to be taken up into the cell in 20 minutes (Mol. Immunol. (2011) 49, 329-337; Science (1992) 256, 1808-1812). Therefore, in humans as well, FcγRIIb2 is presumed to uptake immune complexes into cells at rates similar to those in mice.

[0378] Since FcγRIIb is the only one that has ITIM inside the cell in both humans and mice among the FcγR family and the distribution of expressing cells are the same, it is presumed that its function in immune control is similar. Furthermore, considering the fact that immune complexes are taken up into cells at similar rates in humans and mice, antigen elimination effects of antibodies mediated by FcγRIIb in humans may be predictable using mice. Antigen-binding molecules mF44 and mF46 have properties of binding to soluble antigens in a pH-dependent manner, and have enhanced affinity to mouse FcγRIIb and FcγRIII compared to mIgG1 which is an antigen-binding molecule having the property of binding to a soluble antigen in a pH-dependent manner. Indeed, it is shown in Reference Example 7 that antigen clearance increased when mF44 or mF46 was administered to normal mice compared to when mIgG1 was administered.

[0379] Furthermore, in the later-described Reference Example 8, a similar experiment was carried out using Fc receptor γ chain-deficient mice. It has been reported that FcγRs other than FcγRIIb are expressed only in the co-presence of a gamma chain in mice. Thus, only FcγRIIb is expressed in the Fc receptor γ chain-deficient mice. Administration of mF44 or mF46, which are antigen-binding molecules having the property of binding to soluble antigens in a pH-dependent manner, to Fc receptor γ chain-deficient mice enables assessment of antigen elimination-acceleration effects when FcγRIIb-binding is selectively enhanced. From the results of Reference Example 8, when mF44 or mF46 (which are antigen-binding molecules having the property of binding to soluble antigens in a pH-dependent manner) was administered to Fc receptor γ chain-deficient mice, antigen clearance was shown to increase compared to when mIgG1 (which is an antigen-binding molecule having the property of binding to soluble antigens in a pH-dependent manner) was administered to the mice. Furthermore, the results of Reference Example 8 shows that when administered to Fc receptor γ chain-deficient mice, mF44 or mF46 cause similar degrees of antigen elimination as when administered to normal mice.

[0380] In Reference Example 8, a similar experiment was performed using FcγRIII-deficient mice. Since mIgG1, mF44, and mF46 bind only to FcγRIIb and FcγRIII among the mFcγRs, administration of the antibodies to FcγRIII-deficient mice enables assessment of antigen elimination-accelerating effects when FcγRIIb-binding is selectively enhanced. The results of Reference Example 8 indicate that when mF44 or mF46 was administered to FcγRIII-deficient mice, antigen clearance was increased compared to when mIgG1 was administered to the mice antigen clearance. Furthermore, the results of Reference Example 8 showed that when administered to FcγRIII-deficient mice, mF44 and mF46 cause similar degrees of antigen elimination as when administered to Fc receptor γ chain-deficient mice and when administered to normal mice.

[0381] These results revealed that antigen elimination can be accelerated by selectively enhancing only the binding to FcγRIIb without enhancing the binding to activating FcγRs. More specifically, this shows that FcγRIIb is mainly involved in the FcγR-mediated elimination of immune complexes, and as long as the binding to FcγRIIb among the FcγRs is maintained, the efficiency of FcγR-mediated elimination of immune complexes by the antibody may also be maintained.

[0382] In addition to the reported documents discussed so far, based on the above-mentioned assessment results using mice, it is considered that uptake of immune complexes into cells via FcγRIIb takes place in vivo in humans as in mice, and as a result, antibodies that have Fc with selectively enhanced binding to human FcγRIIb can accelerate elimination of its antigens. Furthermore, as discussed above, since uptake of immune complexes into cells via FcγRIIb is considered to take place at similar rates in mice and humans, effects of accelerating antigen elimination comparable to those of antibodies having Fc with enhanced affinity to mouse FcγRIIb may be achieved in vivo in humans by using Fc in which affinity to human FcγRIIb is enhanced to a similar extent.

[0383] The present invention also provides a method of producing a polypeptide comprising an antibody Fc region variant with decreased binding activities to activating FcγRs while maintaining its FcγRIIb-binding activity, in comparison to those of a polypeptide comprising a parent Fc region, wherein the method comprises adding at least one amino acid alteration to the Fc region variant in a polypeptide comprising the Fc region variant.

[0384] Examples include a production method comprising the following steps:

(a) adding at least one amino acid alteration to an Fc region in a polypeptide comprising the Fc region;
(b) measuring the FcγRIIb-binding activity and binding activities to activating FcγRs of the polypeptide altered in step (a); and
(c) selecting a polypeptide comprising an Fc region variant with decreased binding activities to activating FcγRs while maintaining its FcγRIIb-binding activity in comparison to those of a polypeptide comprising the parent Fc region.

[0385] A preferred embodiment is a method of producing a polypeptide comprising an Fc region variant, which comprises the steps of:

(a) altering a nucleic acid encoding a polypeptide comprising a parent Fc region so that binding activities to activating FcγRs are decreased while the FcγRIIb-binding activity is maintained in comparison to those of the polypeptide;
(b) introducing the nucleic acid into a host cell and culturing the cell to express a polypeptide; and
(c) collecting the polypeptide from the host cell culture.

[0386] Antibodies and Fc fusion protein molecules produced by this production method are also included in this invention.

[0387] Furthermore, the present invention provides methods of decreasing the binding activities to all activating FcγRs, in particular FcγRIIa (R type), while maintaining the FcγRIIb-binding activity compared to those of a polypeptide comprising the parent Fc region, wherein the method comprises the step of adding at least one amino acid alteration to an antibody Fc region variant in a polypeptide comprising the antibody Fc region variant; or a method of producing a polypeptide comprising the Fc region variant of the present invention.

[0388] An example includes a method comprising the following steps of:

(a) adding at least one amino acid alteration to an Fc region in a polypeptide comprising the Fc region;
(b) measuring the FcγRIIa-binding activity and the FcγRIIb-binding activity of the polypeptide altered in step (a); and
(c) selecting a polypeptide comprising an Fc region variant with a decreased binding activity to FcγRIIa (R type) while maintaining its FcγRIIb-binding activity in comparison to those of a polypeptide comprising the parent Fc region.

[0389] A preferred embodiment is a method of decreasing the binding activities to all FcγRs, in particular FcγRIIa (R type), while maintaining the FcγRIIb-binding activity of a polypeptide comprising a parent Fc region, or a method of producing a polypeptide comprising an Fc region variant, which comprises the steps of:

(a) altering a nucleic acid encoding a polypeptide comprising a parent Fc region so that its binding activity to FcγRIIa (R type) is decreased while its FcγRIIb-binding activity is maintained in comparison to those of the polypeptide;
(b) introducing the nucleic acid into a host cell and culturing the cell to express a polypeptide; and
(c) collecting the polypeptide from the host cell culture.

[0390] Furthermore, antibodies and Fc fusion protein molecules produced by the production method are also included in the present invention.

[0391] Furthermore, the present invention provides a method of suppressing production of antibodies against a polypeptide in comparison to a polypeptide comprising a parent Fc region when the polypeptide is administered in vivo, wherein the method comprises adding at least one amino acid alteration to an Fc region in a polypeptide comprising the Fc region, or a method of producing polypeptides, wherein production of antibodies against the polypeptide is suppressed.

[0392] An example includes a method comprising the following steps of:

(a) adding at least one amino acid alteration to an Fc region in a polypeptide comprising the Fc region; and
(b) confirming that administration of the polypeptide comprising the Fc region altered in step (a) in vivo suppresses antibody production as compared to when a polypeptide comprising the parent Fc region is administered.

[0393] Such polypeptides may be useful as pharmaceuticals since they can suppress antibody production without activation of activating FcγRs.

[0394] In the above-mentioned method, it is preferred that the binding activities to all activating FcγRs, in particular FcγRIIa (R type), are decreased while the FcγRIIb-binding activity is maintained.

[0395] In a preferred embodiment of the above-mentioned method, for example, in a human IgG Fc region, the Fc region is altered so that the amino acid at position 238 according to EU numbering is altered to another amino acid, and at least one amino acid selected from among the amino acids at positions 235, 237, 241, 268, 295, 296, 298, 323, 324, and 330 of the Fc region, according to EU numbering, is altered to another amino acid. Two or more amino acids may be selected from the above and combined as the other amino acid alterations to be combined with the amino acid alteration at position 238 according to EU numbering. Preferred combinations include (1) to (3) below:

(1) amino acids at positions 241, 268, 296, and 324 of the Fc region according to EU numbering;
(2) amino acids at positions 237, 241, 296, and 330 of the Fc region according to EU numbering; and
(3) amino acids at positions 235, 237, 241, and 296 of the Fc region according to EU numbering.

[0396] The amino acids selected to be present after alteration are not particularly limited as long as binding selectivity for all activating FcγRs, in particular FcγRIIa(R), are decreased while the FcγRIIb-binding activity is maintained in comparison to those before the alteration, and are preferably Asp at amino acid position 238 according to EU numbering, and also Phe at amino acid position 235, Gln or Asp at amino acid position 237, Met or Leu at amino acid position 241, Pro at amino acid position 268, Met or Val at amino acid position 295, Glu, His, Asn, or Asp at amino acid position 296, Ala or Met at amino acid position 298, Ile at amino acid position 323, Asn or His at amino acid position 324, and His or Tyr at amino acid position 330 according to EU numbering.

[0397] Furthermore, in a preferred embodiment of the above-mentioned method, for example, an Fc region in a human IgG Fc region is altered so that amino acid alteration(s) that increases the FcγRIIb-binding activity by two-fold or more as compared to that of a native IgG Fc region is introduced in combination with amino acid alteration(s) that decreases the binding activities to all FcγRs.

[0398] In the present invention, “amino acid alterations that increase the FcγRIIb-binding activity by two-fold or more as compared to that of a native IgG Fc region” is not particularly limited, but examples include the amino acid alterations shown in Table 11.

[0399] Furthermore, in the present invention, “amino acid alterations that decrease the binding activities to all FcγRs” is not particularly limited, but examples include at least one amino acid selected from among amino acids at positions 234, 235, 236, 237, 239, 265, 267, and 297 of the Fc region according to EU numbering.

[0400] A preferred combination includes, for example, a combination of alterations of the amino acids at positions 238 and 271 of the Fc region according to EU numbering that increase the FcγRIIb-binding activity by two-fold or more compared to that of a native IgG Fc region, with alteration of at least one amino acid selected from among the amino acids at positions 234, 235, 236, 237, 239, 265, 267, and 297 of the Fc region according to EU numbering that reduces the binding activities to all FcγRs.

[0401] Specifically, preferred combinations of alterations include the combinations of amino acids (1) to (3) below:

(1) amino acids at positions 233, 238, 264, 267, 268, and 271 of the Fc region according to EU numbering;
(2) amino acids at positions 233, 237, 238, 264, 267, 268, 271, 296, 297, 330, and 396 of the Fc region according to EU numbering; and
(3) amino acids at positions 233, 238, 264, 267, 268, 271 and 296 of the Fc region according to EU numbering.

[0402] The amino acids selected to be present after alteration are not particularly limited as long as binding selectivities for all activating FcγRs, in particular FcγRIIa(R), are decreased while the FcγRIIb-binding activity is maintained in comparison to those before the alteration, and are preferably Asp at amino acid position 238, Gly at amino acid position 271, Ala, His, Asn, Lys, or Arg at amino acid 234, Ala at amino acid position 235, Gln at amino acid position 236, Arg or Lys at amino acid position 237, Lys at amino acid position 239, Lys, Asn, Arg, Ser, or Val at amino acid position 265, Lys, Arg, or Tyr at amino acid position 267, and Ala at amino acid position 297 according to EU numbering.

[0403] Alternatively, in a preferred embodiment of the above-mentioned method, for example, the Fc region is altered so that alterations of the amino acids at positions 238, 271, 327, 330, and 331 according to EU numbering to other amino acids are introduced into the human IgG Fc region. Furthermore, the Fc region is altered so that alterations of at least one amino acid selected from the amino acids at positions 233, 237, 264, 267, and 268 to other amino acids are introduced. For other amino acid alterations to be combined, two or more amino acids from the above may be selected and combined. Examples of the preferred combination include (1) to (4) below:

(1) amino acids at positions 237, 238, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering;
(2) amino acids at positions 233, 237, 238, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering;
(3) amino acids at positions 238, 267, 268, 271, 327, 330, and 331 of the Fc region according to EU numbering; and
(4) amino acids at positions 238, 264, 267, 271, 327, 330, and 331 of the Fc region according to EU numbering.

[0404] The amino acids selected to be present after alteration are not particularly limited as long as binding selectivities for all activating FcγRs, in particular FcγRIIa(R), are decreased while the FcγRIIb-binding activity is maintained in comparison to before the alteration, and are preferably Asp at amino acid position 238, Gly at amino acid position 271, Gly at amino acid position 327, Ser at amino acid position 330, Ser at amino acid position 331, Asp at amino acid position 233, Asp at amino acid position 237, Ile at amino acid position 264, Ala at amino acid position 267, and Asp or Glu at amino acid position 268 according to EU numbering.

[0405] Furthermore, the present invention provides methods of altering polypeptides for producing polypeptides whose binding activities to activating FcγRs, in particular FcγRIIa (R type), are decreased while the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the parent Fc region. Alternatively, the present invention provides methods of altering polypeptides for producing polypeptides whose binding activities to activating FcγRs, in particular FcγRIIa (R type), are decreased while the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the parent Fc region.

[0406] The present invention also provides methods of altering polypeptides for producing a polypeptide that results in suppressed antibody production when the polypeptide is administered in vivo, in comparison to when a polypeptide comprising the parent Fc region is administered.

[0407] An example of a preferred embodiment includes a combination of amino acid alterations described in the above-described method of producing a polypeptide comprising an Fc region variant with decreased binding activities to activating FcγRs, in particular FcγRIIa (R type), while maintaining its FcγRIIb-binding activity.

[0408] For the various types of methods described above, other amino acid alterations may be used in combination as long as binding activities to all activating FcγRs are decreased and the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the native IgG Fc region. Examples of such alterations include alterations that decrease the complement-binding activity. Specific examples include alteration of the amino acid at position 322 of the Fc region according to EU numbering, or a combination of amino acid alterations at positions 327, 330, and 331 of the Fc region according to EU numbering. The amino acids selected to be present after the alteration are not particularly limited as long as the complement-binding activity is decreased, while binding activities to all activating FcγRs are decreased and the FcγRIIb-binding activity is maintained in comparison to those of a polypeptide comprising the Fc region of a native IgG, and are preferably Ala or Glu at amino acid position 322, Gly at amino acid position 327, Ser at amino acid position 330, and Ser at amino acid position 331 according to EU numbering.

[0409] Furthermore, the present invention provides a nucleic acid encoding a polypeptide comprising an Fc region variant with decreased binding activities to activating FcγRs, in particular FcγRIIa (R type), while maintaining the FcγRIIb-binding activity in comparison to those of a polypeptide comprising the parent Fc region, wherein the Fc region-containing polypeptide has at least one altered amino acid. The present invention also provides a nucleic acid encoding a polypeptide comprising an Fc region variant with decreased binding activities to activating FcγRs, in particular FcγRIIa (R type), while maintaining the FcγRIIb-binding activity in comparison to those of a polypeptide comprising the parent Fc region, wherein the Fc region-containing polypeptide has at least one altered amino acid. The nucleic acids of the present invention may be in any form such as DNA or RNA.

[0410] The present invention also provides vectors carrying the above-described nucleic acids of the present invention. The type of vector can be appropriately selected by those skilled in the art depending on the host cells to be introduced with the vector. The vectors include, for example, those described above.

[0411] Furthermore, the present invention relates to host cells transformed with the above-described vectors of the present invention. Appropriate host cells can be selected by those skilled in the art. The host cells include, for example, those described above. Specific examples include the following host cells.

When eukaryotic cells are used as host cells, animal cells, plant cells, or fungal cells can be appropriately used. Specifically, the animal cells include, for example, the following cells.
(1) mammalian cells: CHO (Chinese hamster ovary cell line), COS (Monkey kidney cell line), myeloma (Sp2/O, NSO, and such), BHK (baby hamster kidney cell line), Hela, Vero, HEK293 (human embryonic kidney cell line with sheared adenovirus (Ad)5 DNA), Freestyle293, PER.C6 cell (human embryonic retinal cell line transformed with the Adenovirus Type 5 (Ad5) E1A and E1B genes), and such (Current Protocols in Protein Science (May, 2001, Unit 5.9, Table 5.9.1));
(2) amphibian cells: Xenopus oocytes, or such; and
(3) insect cells: sf9, sf21, Tn5, or such.

[0412] In addition, as a plant cell, an antibody gene expression system using cells derived from the Nicotiana genus such as Nicotiana tabacum is known. Callus cultured cells can be appropriately used to transform plant cells.

[0413] Furthermore, the following cells can be used as fungal cells:

yeasts: the Saccharomyces genus such as Saccharomyces serevisiae, and the Pichia genus such as Pichia pastoris; and
filamentous fungi: the Aspergillus genus such as Aspergillus niger.

[0414] Furthermore, the present invention provides a method of decreasing binding activities to activating FcγRs, in particular FcγRIIa (R type), while maintaining its FcγRIIb-binding activity in comparison to those of a polypeptide comprising the parent Fc region, which comprises adding at least one amino acid alteration to the Fc region in a polypeptide comprising the Fc region.

[0415] The present invention further provides a method comprising the step of adding at least one amino acid alteration to an Fc region in a polypeptide comprising the Fc region, which is a method that results in suppressed production of antibodies against the polypeptide when the polypeptide is administered in vivo, in comparison to when a polypeptide comprising the parent Fc region is administered.

[0416] A preferred embodiment includes, for example, a combination of amino acid alterations described in the above-described method of producing a polypeptide comprising an Fc region variant with decreased binding activities to activating FcγRs, in particular FcγRIIa (R type), while maintaining its FcγRIIb-binding activity.

[0417] Polypeptides produced by any of the above-mentioned methods are also included in the present invention.

[0418] The present invention provides pharmaceutical compositions comprising the polypeptide comprising an Fc region variant of the present invention.

[0419] The pharmaceutical compositions of the present invention can be formulated, in addition to the antibody or Fc-fusion protein molecules of the present invention described above, with pharmaceutically acceptable carriers by known methods. For example, the compositions can be used parenterally, when the antibodies are formulated in a sterile solution or suspension for injection using water or any other pharmaceutically acceptable liquid. For example, the compositions can be formulated by appropriately combining the antibodies or Fc-fusion protein molecules with pharmaceutically acceptable carriers or media, specifically, sterile water or physiological saline, vegetable oils, emulsifiers, suspending agents, surfactants, stabilizers, flavoring agents, excipients, vehicles, preservatives, binding agents, and such, by mixing them at a unit dose and form required by generally accepted pharmaceutical implementations. Specific examples of the carriers include light anhydrous silicic acid, lactose, crystalline cellulose, mannitol, starch, carmellose calcium, carmellose sodium, hydroxypropyl cellulose, hydroxypropyl methylcellulose, polyvinylacetal diethylaminoacetate, polyvinylpyrrolidone, gelatin, medium-chain triglyceride, polyoxyethylene hardened castor oil 60, saccharose, carboxymethyl cellulose, corn starch, inorganic salt, and such. The content of the active ingredient in such a formulation is adjusted so that an appropriate dose within the required range can be obtained.

[0420] Sterile compositions for injection can be formulated using vehicles such as distilled water for injection, according to standard protocols.

[0421] Aqueous solutions used for injection include, for example, physiological saline and isotonic solutions containing glucose or other adjuvants such as D-sorbitol, D-mannose, D-mannitol, and sodium chloride. These can be used in conjunction with suitable solubilizers such as alcohol, specifically ethanol, polyalcohols such as propylene glycol and polyethylene glycol, and non-ionic surfactants such as Polysorbate 80™ and HCO-50.

[0422] Oils include sesame oils and soybean oils, and can be combined with solubilizers such as benzyl benzoate or benzyl alcohol. These may also be formulated with buffers, for example, phosphate buffers or sodium acetate buffers; analgesics, for example, procaine hydrochloride; stabilizers, for example, benzyl alcohol or phenol; or antioxidants. The prepared injections are typically aliquoted into appropriate ampules.

[0423] The administration is preferably carried out parenterally, and specifically includes injection, intranasal administration, intrapulmonary administration, and percutaneous administration. For example, injections can be administered systemically or locally by intravenous injection, intramuscular injection, intraperitoneal injection, or subcutaneous injection.

[0424] Furthermore, the method of administration can be appropriately selected according to the age and symptoms of the patient. A single dosage of the pharmaceutical composition containing an antibody or a polynucleotide encoding an antibody can be selected, for example, from the range of 0.0001 mg to 1000 mg per kg of body weight. Alternatively, the dosage may be, for example, in the range of 0.001 to 100000 mg/body. However, the dosage is not limited to these values. The dosage and method of administration vary depending on the patient's body weight, age, and symptoms, and can be appropriately selected by those skilled in the art.

[0425] The above-mentioned polypeptides comprising an Fc region variant of the present invention are useful as active ingredients of pharmaceutical agents that suppress the activation of B cells, mast cells, dendritic cells, and/or basophils. Polypeptides comprising an Fc region variant of the present invention can suppress the activation of B cells, mast cells, dendritic cells, and/or basophils, by selectively working on FcγRIIb without activating FcγRs. B cell activation includes proliferation, IgE production, IgM production, and IgA production. The above-mentioned polypeptides comprising an Fc region variant of the present invention cross-link FcγRIIb with IgE to suppress IgE production of B cells, with IgM to suppress IgM production of B cells, and with IgA to suppress IgA production. Other than the above, suppressive effects similar to those mentioned above are exhibited by directly or indirectly cross-linking FcγRIIb with molecules that are expressed on B cells and comprise the ITAM domain inside the cell or interact with the ITAM domain such as BCR, CD19, and CD79b. Furthermore, activation of mast cells includes proliferation, activation by IgE and such, and degranulation. In mast cells, the above-mentioned polypeptides comprising an Fc region variant of the present invention can suppress proliferation, activation by IgE and such, and degranulation by directly or indirectly cross-linking FcγRIIb with IgE receptor molecules that are expressed on mast cells and comprise the ITAM domain or interact with the ITAM domain such as FcεRI, DAP12, and CD200R3. Activation of basophils includes proliferation and degranulation of basophils. Also in basophils, the above-mentioned polypeptides comprising an Fc region variant of the present invention can suppress proliferation, activation, and degranulation by directly or indirectly cross-linking FcγRIIb with molecules on the cell membrane, which comprise the ITAM domain inside the cell or interact with the ITAM domain. Activation of dendritic cells includes proliferation and degranulation of dendritic cells. Also in dendritic cells, the above-mentioned polypeptides comprising an Fc region variant of the present invention can suppress activation, degranulation, and proliferation by directly or indirectly cross-linking FcγRIIb with molecules on the cell membrane, which comprise the ITAM domain inside the cell or interact with the ITAM domain.

[0426] In the present invention, the polypeptides comprising an Fc region variant of the present invention mentioned above are useful as an active ingredient of therapeutic agents or preventive agents for immunological inflammatory diseases. As described above, since polypeptides comprising an Fc region variant of the present invention can suppress activation of B cells, mast cells, dendritic cells and/or basophils, administration of the polypeptides comprising an Fc region variant of the present invention as a result can treat or prevent immunological inflammatory diseases. Without being limited thereto, the term “immunological inflammatory diseases” comprises, rheumatoid arthritis, autoimmune hepatitis, autoimmune thyroiditis, autoimmune blistering diseases, autoimmune adrenocortical disease, autoimmune hemolytic anemia, autoimmune thrombocytopenic purpura, megalocytic anemia, autoimmune atrophic gastritis, autoimmune neutropenia, autoimmune orchitis, autoimmune encephalomyelitis, autoimmune receptor disease, autoimmune infertility, chronic active hepatitis, glomerulonephritis, interstitial pulmonary fibrosis, multiple sclerosis, Paget's disease, osteoporosis, multiple myeloma, uveitis, acute and chronic spondylitis, gouty arthritis, inflammatory bowel disease, adult respiratory distress syndrome (ARDS), psoriasis, Crohn's disease, Basedow's disease, juvenile diabetes, Addison's disease, myasthenia gravis, lens-induced uveitis, systemic lupus erythematosus, allergic rhinitis, allergic dermatitis, ulcerative colitis, hypersensitivity, muscle degeneration, cachexia, systemic scleroderma, localized scleroderma, Sjogren's syndrome, Behchet's disease, Reiter's syndrome, type I and type II diabetes, bone resorption disorder, graft-versus-host reaction, ischemia-reperfusion injury, atherosclerosis, brain trauma, cerebral malaria, sepsis, septic shock, toxic shock syndrome, fever, malgias due to staining, aplastic anemia, hemolytic anemia, idiopathic thrombocytopenia, Goodpasture's syndrome, Guillain-Barre syndrome, Hashimoto's thyroiditis, pemphigus, IgA nephropathy, pollinosis, antiphospholipid antibody syndrome, polymyositis, Wegener's granulomatosis, arteritis nodosa, mixed connective tissue disease, fibromyalgia, asthma, atopic dermatitis, chronic atrophic gastritis, primary biliary cirrhosis, primary sclerosing cholangitis, autoimmune pancreatitis, aortitis syndrome, rapidly progressive glomerulonephritis, megaloblastic anemia, idiopathic thrombocytopenic purpura, primary hypothyroidism, idiopathic Addison's disease, insulin-dependent diabetes mellitus, chronic discoid lupus erythematosus, pemphigoid, herpes gestationis, linear IgA bullous dermatosis, epidermolysis bullosa acquisita, alopecia areata, vitiligo vulgaris, leukoderma acquisitum centrifugum of Sutton, Harada's disease, autoimmune optic neuropathy, idiopathic azoospermia, habitual abortion, hypoglycemia, chronic urticaria, ankylosing spondylitis, psoriatic arthritis, enteropathic arthritis, reactive arthritis, spondyloarthropathy, enthesopathy, irritable bowel syndrome, chronic fatigue syndrome, dermatomyositis, inclusion body myositis, Schmidt's syndrome, Graves' disease, pernicious anemia, lupoid hepatitis, presenile dementia, Alzheimer's disease, demyelinating disorder, amyotrophic lateral sclerosis, hypoparathyroidism, Dressler's syndrome, Eaton-Lambert syndrome, dermatitis herpetiformis, alopecia, progressive systemic sclerosis, CREST syndrome (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, and telangiectasia), sarcoidosis, rheumatic fever, erythema multiforme, Cushing's syndrome, transfusion reaction, Hansen's disease, Takayasu arteritis, polymyalgia rheumatica, temporal arteritis, giant cell arthritis, eczema, lymphomatoid granulomatosis, Kawasaki disease, endocarditis, endomyocardial fibrosis, endophthalmitis, fetal erythroblastosis, eosinophilic fasciitis, Felty syndrome, Henoch-Schonlein purpura, transplant rejection, mumps, cardiomyopathy, purulent arthritis, familial Mediterranean fever, Muckle-Wells syndrome, and hyper-IgD syndrome.

[0427] Furthermore, in autoimmune diseases which may be caused by production of antibodies against autoantigens (autoantibodies), the polypeptides comprising an Fc region variant of the present invention mentioned above are useful as an active ingredient of pharmaceutical agents for treating or preventing the autoimmune diseases by suppressing production of those autoantibodies. Use of a molecule produced by fusing an antibody Fc portion with AchR (an autoantigen of myasthenia gravis) has been reported to suppress proliferation of B cells which express AchR-recognizing BCR, and induce apoptosis (J. Neuroimmunol, 227: 35-43, 2010). Use of a fusion protein formed between an antigen recognized by an autoantibody and an antibody Fc region of the present invention enables crosslinking of FcγRIIb with BCR of a B cell expressing BCR for that autoantigen, suppression of proliferation of B cells expressing BCR for the autoantigen, and induction of apoptosis. Such autoimmune diseases include Guillain-Barre syndrome, myasthenia gravis, chronic atrophic gastritis, autoimmune hepatitis, primary biliary cirrhosis, primary sclerosing cholangitis, autoimmune pancreatitis, aortitis syndrome, Goodpasture's syndrome, rapidly progressive glomerulonephritis, megaloblastic anemia, autoimmune hemolytic anemia, autoimmune neutropenia, idiopathic thrombocytopenic purpura, Basedow's disease, Hashimoto's thyroiditis, primary hypothyroidism, idiopathic Addison's disease, insulin-dependent diabetes mellitus, chronic discoid lupus erythematosus, localized scleroderma, pemphigus, pemphigoid, herpes gestationis, linear IgA bullous dermatosis, epidermolysis bullosa acquisita, alopecia areata, vitiligo vulgaris, leukoderma acquisitum centrifugum of Sutton, Harada's disease, autoimmune optic neuropathy, idiopathic azoospermia, habitual abortion, type II diabetes, hypoglycemia, and chronic urticaria; but are not limited thereto.

[0428] Furthermore, the above-mentioned polypeptides comprising an Fc Region variant of the present invention are useful as an active ingredient in therapeutic agents for diseases with deficiency of a biologically essential protein. For diseases with deficiency of a biologically essential protein, therapeutic methods that administer and supplement the protein as a pharmaceutical agent are used. However, since the patient lacks the protein from the beginning, the externally supplemented protein is recognized as a foreign substance and antibodies against that protein are produced. As a result, the protein becomes easily removed, and the effect as a pharmaceutical is reduced. Use of a fusion protein comprising such a protein and an antibody Fc region of the present invention enables crosslinking between FcγRIIb and BCR on B cells that recognize the protein, and enables suppression of antibody production against the protein. The proteins to be supplemented include Factor VIII, Factor IX, TPO, EPO, α-iduronidase, iduronate sulfatase, A-type heparan N-sulfatase, B type α-N-acetylglucosaminidase, C type acetyl CoA: α-glucosaminidase acetyltransferase, D type N-acetylglucosamine 6-sulfatase, galactose 6-sulfatase, N-acetylgalactosamine 4-sulfatase, β-glucuronidase, α-galactosidase, acidic α-galactosidase, and glucocerebrosidase. These proteins may be supplemented for diseases such as hemophilia, idiopathic thrombocytopenic purpura, renal anemia, and lysosomal disease (mucopolysaccharidosis, Fabry's disease, Pompe disease, and Gaucher's disease), without being limited thereto.

[0429] Furthermore, the above-mentioned polypeptides comprising an Fc region variant of the present invention are useful as an active ingredient for antiviral agents. Antibodies that comprise an Fc region of the present invention and are anti-virus antibodies can suppress antibody-dependent enhancement observed with anti-virus antibodies. Antibody-dependent enhancement is a phenomenon where a virus uses neutralizing antibodies against the virus to become phagocytosed via activating FcγRs, and infects FcγR-expressing cells so that the infection spreads. Binding of anti-dengue-virus neutralizing antibodies to FcγRIIb has been reported to play an important role in suppressing antibody-dependent enhancement (Proc. Natl. Acad. Sci. USA, 108: 12479-12484, 2011). Crosslinking FcγRIIb with an immune complex with dengue virus, which is formed by the anti-dengue-virus neutralizing antibodies, inhibits FcγR-mediated phagocytosis, resulting in the suppression of antibody-dependent enhancement. Examples of such viruses include dengue virus (DENV1, DENV2, and DENV4) and HIV, but are not limited thereto.

[0430] Furthermore, polypeptides comprising an Fc region variant of the present invention described above are useful as an active ingredient in preventive agents or therapeutic agents for arteriosclerosis. Antibodies against oxidized LDL, i.e., a cause for arteriosclerosis, which are antibodies comprising an Fc region of the present invention, can prevent FcγRIIa-dependent adhesion of inflammatory cells. It has been reported that while anti-oxidized LDL antibodies inhibit the interaction between oxidized LDL and CD36, anti-oxidized LDL antibodies bind to endothelial cells, and monocytes recognize their Fc portion in an FcγRIIa-dependent or FcγRI-dependent manner; and this leads to adhesion (Immunol. Lett., 108: 52-61, 2007). Using antibodies comprising an Fc region of the present invention for such antibodies may inhibit FcγRIIa-dependent binding and suppress monocyte adhesion by FcγRIIb-mediated inhibitory signals.

[0431] In the present invention, the above-mentioned polypeptides comprising the Fc region variants of the present invention are useful as active ingredients for therapeutic agents or prophylactic agents against cancer. As described above, by decreasing binding to all activating FcγRs and maintaining binding to FcγRIIb, platelet activation through the FcγRIIa-dependent mechanism can be suppressed while maintaining agonistic activity of agonist antibodies, and risk of thromboembolism and such may be reduced. Therefore, agonist antibodies using the Fc region variant of the present invention are useful for treatment or prevention of cancer. Specifically, the Fc region variant of the present invention enhances the agonistic activity of agonist antibodies against, for example, receptors of the TNF receptor family such as Aliases, CD120a, CD120b, Lymphotoxin β receptor, CD134, CD40, FAS, TNFRSF6B, CD27, CD30, CD137, TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, RANK, Osteoprotegerin, TNFRSF12A, TNFRSF13B, TNFRSF13C, TNFRSF14, Nerve growth factor receptor, TNFRSF17, TNFRSF18, TNFRSF19, TNFRSF21, TNFRSF25, and Ectodysplasin A2 receptor and can be used for treating or preventing cancer. Furthermore, in addition to the above, agonistic activity is also enhanced for agonist antibodies against molecules which need to interact with FcγRIIb for exhibiting its agonistic activity. In addition, by incorporating the Fc region variant of the present invention into a polypeptide having binding activity to a molecule such as Kit, a type of receptor tyrosine kinase (RTK), which suppresses cell proliferation upon crosslinking with FcγRIIb, inhibitory effect against cells expressing such molecule may be enhanced. Without being limited thereto, cancer includes lung cancer (including small cell lung cancer, non-small cell lung cancer, pulmonary adenocarcinoma, and squamous cell carcinoma of the lung), large intestine cancer, rectal cancer, colon cancer, breast cancer, liver cancer, gastric cancer, pancreatic cancer, renal cancer, prostate cancer, ovarian cancer, thyroid cancer, cholangiocarcinoma, peritoneal cancer, mesothelioma, squamous cell carcinoma, cervical cancer, endometrial cancer, bladder cancer, esophageal cancer, head and neck cancer, nasopharyngeal cancer, salivary gland tumor, thymoma, skin cancer, basal cell tumor, malignant melanoma, anal cancer, penile cancer, testicular cancer, Wilms' tumor, acute myeloid leukemia (including acute myeloleukemia, acute myeloblastic leukemia, acute promyelocytic leukemia, acute myelomonocytic leukemia, and acute monocytic leukemia), chronic myelogenous leukemia, acute lymphoblastic leukemia, chronic lymphatic leukemia, Hodgkin's lymphoma, non-Hodgkin's lymphoma (Burkitt's lymphoma, chronic lymphocytic leukemia, mycosis fungoides, mantle cell lymphoma, follicular lymphoma, diffuse large-cell lymphoma, marginal zone lymphoma, pilocytic leukemia plasmacytoma, peripheral T-cell lymphoma, and adult T cell leukemia/lymphoma), Langerhans cell histiocytosis, multiple myeloma, myelodysplastic syndrome, brain tumor (including glioma, astroglioma, glioblastoma, meningioma, and ependymoma), neuroblastoma, retinoblastoma, osteosarcoma, Kaposi's sarcoma, Ewing's sarcoma, angiosarcoma, and hemangiopericytoma.

[0432] Furthermore, the present invention relates to methods for treating or preventing immunological inflammatory diseases, which comprise the step of administering to a subject (patient) a polypeptide comprising an Fc region variant of the present invention or a polypeptide comprising an Fc region variant produced by production methods of the present invention.

[0433] The present invention also provides kits for use in the therapeutic methods or preventive methods of the present invention, which comprises at least a polypeptide comprising an Fc region variant of the present invention or a polypeptide comprising an Fc region variant produced by production methods of the present invention, or a pharmaceutical composition of the present invention. In addition, pharmaceutically acceptable carriers, media, instructions on the method of use, and such may be included in the kit. Furthermore, the present invention relates to use of a polypeptide comprising an Fc region variant of the present invention or a polypeptide comprising an Fc region variant produced by production methods of the present invention in the production of agents for treating or preventing immunological inflammatory diseases. The present invention also relates to polypeptides comprising an Fc region variant of the present invention or polypeptides comprising an Fc region variant produced by production methods of the present invention for use in the therapeutic methods or preventive methods of the present invention.

[0434] As used herein, the three-letter and single-letter codes for respective amino acids are as follows:

[0435] Aspartic acid: Asp (D)

[0436] Glutamic acid: Glu (E)

[0437] All prior art documents cited herein are incorporated by reference in their entirety.

[0438] Herein below, the present invention will be specifically described further with reference to the Examples, but it is not to be construed as being limited thereto.

[0439] In order to prepare a pH-dependent anti-human IgE antibody, human IgE (heavy chain, SEQ ID NO: 13; light chain, SEQ ID NO: 14) (its variable region is from an anti-human glypican 3 antibody) was expressed as an antigen using FreeStyle293 (Life Technologies). The expressed human IgE was prepared and purified by a general column chromatographic method known to those skilled in the art.

[0440] Antibodies that form large immune complexes comprising two or more anti-IgE antibody molecules and two or more IgE molecules, which show pH-dependent binding to human IgE, were selected from among the multiple antibodies obtained. Expression and purification of the selected anti-human IgE antibodies were carried out using the human IgG1 heavy chain constant regions and the human light chain constant regions. The produced antibody was named clone 278 (IgG1) (heavy chain SEQ ID NO: 10, light chain SEQ ID NO: 11).

(1-2) Evaluation of Binding Activities and pH-Dependent Binding Activities of Anti-Human IgE Antibodies

[0441] Antibodies that can release antigens in the endosome can be produced using not only pH-dependent antigen binding but also Ca-dependent binding. Accordingly, the pH-dependent ability and pH/Ca-dependent ability to bind to human IgE (hIgE) were evaluated for clone 278 and Xolair (omalizumab, Novartis) used as a control which does not have pH-dependent IgE-binding ability.

[0442] More specifically, hIgE-binding activities (dissociation constant K_D (M)) of clone 278 and Xolair were evaluated using Biacore T200 (GE Healthcare). Measurements were carried out using the following three types as running buffer:

[0443] 1.2 mmol/l CaCl₂/0.05% tween20, 20 mmol/l ACES, 150 mmol/l NaCl, pH 7.4

[0444] 1.2 mmol/l CaCl₂/0.05% tween20, 20 mmol/l ACES, 150 mmol/l NaCl, pH 5.8

[0445] 3 μmol/l CaCl₂/0.05% tween20, 20 mmol/l ACES, 150 mmol/l NaCl, pH 5.8

[0446] A peptide produced by adding biotin to Lys present at the C terminus of a chemically synthesized human glypican 3 protein-derived sequence (SEQ ID NO: 12) (hereinafter written as “biotinylated GPC3 peptide”) added at an appropriate amount was immobilized onto Sensor chip SA (GE Healthcare) utilizing the affinity between streptavidin and biotin. An appropriate concentration of human IgE was injected and trapped with the biotinylated GPC3 peptide to immobilize human IgE onto the chip. An appropriate concentration of clone 278 was injected as an analyte, and allowed to interact with human IgE on the sensor chip. Then, 10 mmol/L glycine-HCl (pH 1.5) was injected to regenerate the sensor chip. All of the assay for the interaction was performed at 37° C. Using Biacore T200 Evaluation Software (GE Healthcare), the assay results were analyzed by curve fitting to determine the binding rate constant ka (1/Ms) and dissociation rate constant kd (1/s). Dissociation constant KD (M) was calculated from the above constants. Then, the pH-dependent binding was assessed by calculating the KD ratio of each antibody between the conditions of pH 5.8/1.2 mM Ca and pH 7.4/1.2 mM Ca. The pH/Ca-dependent binding was assessed by calculating the KD ratio of each antibody between the conditions of pH 5.8/3 μM Ca and pH 7.4/1.2 mM Ca. The results are shown in Table 5.

TABLE 5
					pH dependency	pH/Ca dependency
Antibody name		ka	kd	KD	KD(ph 5.8, 1.2 mM Ca)/	KD(ph 5.8, 3 μM Ca)/
(abbreviated)	Buffer condition	(1/Ms)	(1/s)	(M)	KD(pH 7.4, 1.2 mM Ca)	KD(pH 7.4, 1.2 mM Ca)
Clone 278	pH 7.4, 1.2 mM Ca	1.5E+06	3.6E−03	2.4E−09	842.5	1636.5
	pH 5.8, 1.2 mM Ca	1.2E+05	2.3E−01	2.0E−06
	pH 5.8, 3 μM Ca	6.2E+04	2.4E−01	3.9E−06
Xolair	pH 7.4, 1.2 mM Ca	2.5E+06	1.1E−02	4.4E−09	2.3	2.9
	pH 5.8, 1.2 mM Ca	2.4E+06	2.4E−02	9.9E−09
	pH 5.8, 3 μM Ca	1.4E+06	1.7E−02	1.3E−08

(1-3) Evaluation of the Formation of Immune Complexes with Clone 278 and Xolair

[0447] Formation of a large immune complex comprising two or more anti-IgE antibody molecules and two or more IgE molecules under a neutral condition (pH 7.4) by clone 278 and human IgE, and dissociation of that immune complex under an acidic condition (pH 5.8) were evaluated by gel filtration chromatography. Clone 278 dialyzed against 100 mM NaCl was diluted using a 20 mM Tris-HCl, 150 mM NaCl, 1.2 mM CaCl₂, pH 7.4 buffer to prepare samples under neutral conditions, or a 20 mM Bis-tris-HCl, 150 mM NaCl, 3 μM CaCl₂, pH 5.8 buffer to prepare samples under acidic conditions. Mixed solutions produced by mixing 100 μg/mL (0.06 μM) of hIgE (Asp6), which is a human IgE, and clone 278 at 1:1 and 1:6 molar ratios were left to stand at room temperature or in a 25° C. auto-sampler for two hours or longer, and then they were analyzed by gel filtration chromatography. For the mobile phase, 20 mM Tris-HCl, 300 mM NaCl, 1.2 mM CaCl₂, pH 7.4 and 20 mM Bis-tris-HCl, 300 mM NaCl, 3 μM CaCl₂, pH 5.8 were used under a neutral condition and an acidic condition, respectively. G4000SWxl (TOSOH) was used for the column and analyses were carried out at a flow rate of 0.5 mL/min at 25° C. The results are shown in FIG. 1. As shown in FIG. 1, clone 278 and human IgE were found to form large immune complexes comprising a tetramer having an apparent molecular weight of approximately 670 kDa (supposing that a single antibody molecule is a monomer) and larger multimers under a neutral condition. Such immune complexes were not observed under acidic conditions. Therefore, in a similar manner to the above-mentioned evaluation of binding using Biacore, these immune complexes were found to show pH-dependent dissociation.

[0448] (2-1) Preparation of Human IgE (hIgE (Asp6)) for In Vivo Assessment

[0449] hIgE (Asp6) (its variable region is from an anti-human glypican 3 antibody), which is a human IgE for in vivo assessment, comprising the heavy chain (SEQ ID NO: 15) and light chain (SEQ ID NO: 14), was prepared by the same method as described in Example 1. hIgE (Asp6) is a molecule in which asparagine has been replaced with aspartic acid in the six N-glycosylation sites in human IgE, so that time-dependent changes in the concentration of human IgE as an antigen in the plasma does not affect the heterogeneity of N-linked sugar chains of human IgE.

[0450] To C57BL/6J mice (Charles river Japan), hIgE (Asp6) alone was administered, or hIgE (Asp6) and an anti-hIgE antibody (clone 278 or Xolair) were administered simultaneously, and then the in vivo kinetics of hIgE (Asp6) and anti-human IgE antibodies were evaluated. hIgE (Asp6) (20 μg/mL) or a mixed solution of hIgE (Asp6) and the anti-human IgE antibody (concentrations are shown in Table 6) was administered once at 10 mL/kg from the tail vein. Since each antibody is present sufficiently in excess with respect to hIgE (Asp6) at this point, most of the hIgE (Asp6) is considered to be bound to the antibody. Blood was collected from the mice 5 minutes, 2 hours, 7 hours, 1 day, 2 days, 4 days or 5 days, 7 days, 14 days, 21 days, and 28 days after administration. The collected blood samples were immediately subjected to centrifugation at 15,000 rpm for 5 minutes at 4° C. to obtain the plasma. The separated plasma samples were stored in a freezer set to −20° C. or lower until measurements were performed.

TABLE 6
	hIgE (Asp6) concentration in	Anti-hIgE antibody
Anti-hIgE	the administered solution	concentration in the
antibody	(μg/mL)	administered solution (μg/mL)
Clone 278	20	100
Xolair	20	308

(2-3) Measurement of Plasma hIgE (Asp6) Concentration in Normal Mice

[0451] Plasma hIgE (Asp6) concentrations in mice were measured by ELISA. Calibration curve samples were prepared at plasma concentrations of 192, 96, 48, 24, 12, 6, and 3 ng/mL. To make the immune complexes formed between hIgE (Asp6) and an anti-hIgE antibody homogeneous, Xolair (Novartis) was added to the calibration curve samples and the mouse plasma measurement samples at 10 μg/mL, and these were left to stand at room temperature for 30 minutes. Subsequently, the calibration curve samples and the mouse plasma measurement samples were dispensed onto an anti-human IgE-immobilized immunoplate (MABTECH) or an anti-human IgE (clone 107, MABTECH)-immobilized immunoplate (Nunc F96 MicroWell Plate (Nalge nunc International)), and this was left to stand for two hours at room temperature or overnight at 4° C. Thereafter, the human GPC3 core protein (SEQ ID NO: 16), an anti-GPC3 antibody (prepared in-house) biotinylated with NHS-PEG4-Biotin (Thermo Fisher Scientific), and Sterptavidin-PolyHRP80 (Stereospecific Detection Technologies) were individually reacted for one hour in a sequential manner. Concentrations in mouse plasma were measured using a method of measuring the absorbance at 450 nm with a microplate reader for color developed in a coloring reaction that uses TMB One Component HRP Microwell Substrate (BioFX Laboratories) as a substrate after the reaction is stopped with 1N-Sulfuric acid (Showa Chemical), or a method of measuring the luminescence intensity with a microplate reader after a luminescent reaction performed using SuperSignal® ELISA Pico Chemiluminescent Substrate (Thermo Fisher Scientific) as the substrate. The concentrations in mouse plasma were calculated from a calibration curve of luminescence intensity or absorbance values using the SOFTmax PRO analysis software (Molecular Devices). The change in plasma hIgE (Asp6) concentration after intravenous administration measured by this method is shown in FIG. 2. In the figure, clone 278 is noted as 278-IgG1 and Xolair as Xolair-IgG1.

[0452] The anti-hIgE antibody concentration in mouse plasma was measured by ELISA. Calibration curve samples were prepared at plasma concentrations of 0.4, 0.2, 0.1, 0.05, 0.025, 0.0125, and 0.00625 μg/mL. To homogenize the immune complexes formed between hIgE (Asp6) and an anti-hIgE antibody, the calibration curve samples and the mouse plasma measurement samples were left to stand at room temperature for 30 minutes after addition of hIgE (Asp6) at 1 μg/mL. Subsequently, the calibration curve samples and the mouse plasma measurement samples were dispensed onto an anti-human kappa light chain antibody (Bethyl Laboratories)-immobilized immunoplate (Nunc-Immuno Plate, MaxiSorp (Nalge nunc International)), and this was left to stand for two hours at room temperature or overnight at 4° C. Thereafter, a rabbit anti-human IgG (Fc) secondary antibody, biotin conjugate (Pierce Biotechnology), and Streptavidin-PolyHRP80 (Stereospecific Detection Technologies) were individually reacted for one hour in a sequential manner. Concentrations in mouse plasma were determined using a method of measuring the absorbance at 450 nm with a microplate reader for color developed in a coloring reaction that uses TMB One Component HRP Microwell Substrate (BioFX Laboratories) as substrate after the reaction is stopped with 1N-sulfuric acid (Showa Chemical). The concentrations in mouse plasma were calculated from a calibration curve of absorbance values using the SOFTmax PRO analysis software (Molecular Devices). The change in plasma IgE antibody concentration after intravenous administration measured by this method is shown in FIG. 3. In the figure, clone 278 is noted as 278-IgG1 and Xolair as Xolair-IgG1.

[0453] As a result, when human IgE and Xolair which is the control anti-IgE antibody were administered simultaneously, elimination of human IgE became slow in comparison to elimination when human IgE was administered alone. On the other hand, with simultaneous administration of clone 278 which has a pH-dependent binding activity to human IgE, elimination of human IgE was found to be greatly accelerated compared to when human IgE was administered alone.

[0454] In WO2011/122011, a similar experiment has been performed using human IL-6 receptor (hsIL-6R) and antibodies that bind to hsIL-6R, but the results were different from the results of the present Examples. In Example 3 of WO2011/122011, when an IgG1 antibody which is an antibody (H54L28-IgG1) that binds to the human IL-6 receptor (hsIL-6R) but not in a pH-dependent manner had been administered simultaneously with an antigen to a normal mouse (C57BL/6J mouse), elimination of the human IL-6 receptor (the antigen) was not accelerated and was in fact slowed down in comparison to the case when the antigen alone was administered. When an antibody (Fv4-IgG1) that shows pH-dependent antigen binding had been administered simultaneously with an antigen, antigen elimination was slowed down in comparison to when the antigen alone was administered while antigen elimination was accelerated in comparison to when H54L28-IgG1 had been administered. This may have taken place since the antibody administration resulted in binding of the antibody to the antigen, which caused the antigen to move with the antibody, become recycled via FcRn in the same way as the antibody, and caused elimination from the blood to become difficult.

[0455] The result obtained from the present Examples is that simultaneous administration of an antigen with an antibody accelerates antigen elimination in comparison to when the antigen is administered alone, and appears to contradict previous reports. However, IgE used in the Examples as the antigen is a divalent antigen, and is different in the aspect that hsIL-6R is a monovalent antigen. When an anti-IgE antibody is added to IgE, a single antigen may be bound by two antibodies, and this leads to formation of an immune complex comprising multiple antigens and antibodies as observed in Example (1-3). By itself the antibody can only bind monovalently (with affinity) to FcγR which is an IgG receptor, but in the case of the above-described immune complex comprising multiple antigens and antibodies, it is possible to bind multivalently (with avidity) to FcγR. On the other hand, in the case of hsIL-6R, a single antigen can be bound by only one antibody; therefore, an immune complex comprising that antigen and antibody can only bind with FcγR monovalently (with affinity), and the interaction is very weak in comparison to when binding takes place with avidity. More specifically, an immune complex formed by IgE and its antibody binds strongly to FcγR with avidity, and as a result, it could be removed quickly from the blood via the liver or such that expresses FcγR.

[0456] Furthermore, when an antibody binds to the antigen, IgE, in a pH-dependent manner, the antigen-antibody immune complex is taken up into cells, and then the antigen dissociates in the endosome. Then, the antigen is not recycled with the antibody via FcRn, and the dissociated antigen is degraded in the lysosome. As a result, the antigen is considered to be eliminated more rapidly in comparison to when the antigen-antibody binding is not pH dependent.

[0457] (3-1) Obtaining Anti-Human IgE Antibodies with Reduced FcγR Binding

[0458] Next, to verify whether the acceleration of antigen elimination observed in Example 2 comes from interaction between the immune complex and FcγR, a variant that has reduced binding to mouse FcγRs was produced from 278-IgG1 which binds to human IgE in a pH-dependent manner. To lower the binding to mouse FcγRs, Leu at position 235 was substituted with Arg, and Ser at position 239 was substituted with Lys in 278-IgG1, according to EU numbering, to produce 278-F760 (light chain SEQ ID NO: 11). DNA sequences encoding these genes were inserted into plasmids for expression in animals by a method known to those skilled in the art. These antibody variants expressed by the above-mentioned method using the animal cells introduced with the plasmids were purified, and then their concentrations were determined.

(3-2) Verification of the Effect of Xolair and Clone 278 which has Reduced FcγR Binding to Accelerate Human IgE Elimination Using Normal Mice

[0459] By a method similar to that of (2-2), normal mice were used to verify the IgE elimination effect obtained when 278-F760 (light chain SEQ ID NO: 11) which has reduced binding to mouse FcγR was administered.

(3-3) Measurement of Plasma hIgE (Asp6) Concentration in Normal Mice

[0460] Plasma hIgE concentrations in normal mice were measured by a method similar to that of (2-3). The change in plasma hIgE concentration after intravenous administration measured by this method is shown in FIG. 4. For comparison, the change in plasma hIgE concentration upon administration of 278-IgG1 obtained in (2-3) is also shown in the figure.

[0461] The plasma anti-hIgE antibody concentration in normal mice was measured by a method similar to that of (2-4).

[0462] The change in plasma antibody concentration after intravenous administration measured by this method is shown in FIG. 5. For comparison, the change in plasma antibody concentration regarding 278-IgG1 obtained in (2-3) is also shown in the figure.

[0463] In the results of the present Examples, no major change in the shift of plasma antibody concentration was observed by reducing antibody binding to FcγR; however, the antigen elimination-accelerating effect of clone 278 upon administration of IgG1 antibody observed in Example 2 was remarkably attenuated. More specifically, acceleration of IgE elimination observed upon simultaneous administration with an anti-IgE antibody in Example 2 was shown to be derived from interaction between the administered antibody and FcγR.

[0464] Accordingly, to efficiently remove target antigens using antibodies, it may be necessary to form an immune complex comprising multiple antigens and antibodies, maintain FcγR binding of the antibody to a degree similar to that of a native IgG1 antibody, and preferably have pH-dependent binding of the antibody to antigen (antigen binding under acidic pH conditions is lowered in comparison to the binding under neutral conditions).

[0465] (4-1) Investigation of Alterations to be Combined with the P238D Alteration to Maintain Binding to FcgRIIb and Lower Binding to FcgRIIaR

[0466] In the production of an antibody with selectively lowered binding only to activating FcgRs and while binding to FcgRIIb is maintained at a level similar to that of a native IgG1, the most difficult problem is to differentiate between FcgRIIa and FcgRIIb which have very high amino acid sequence homology in order to selectively reduce the binding activity. FcgRIIa has polymorphic forms, one where the amino acid at position 131 is Arg, and the other where this amino acid is His. The residue in FcgRIIb corresponding to this residue is Arg, and therefore, the sequence of FcgRIIb is more similar to the FcgRIIa R type sequence. Therefore, between the FcgRIIas, differentiating the FcgRIIa R type from FcgRIIb was considered to be a particularly difficult problem. As an alteration for improving binding selectivity for FcgRIIb over FcgRIIaR, the alteration of substituting Asp for Pro at position 238 according to EU numbering has been reported in WO2012/115241. The present investigation aims to use an antibody containing this alteration as the template to produce variants whose binding to FcgRIIb is maintained at a level similar to that of the native IgG1 while binding to other FcgRs is attenuated as much as possible.

[0467] Based on the results of X ray crystal structure analyses of a complex formed between Fc(P238D) and the extracellular region of FcγRIIb obtained in Example 5 of WO2012/115241, alterations were introduced comprehensively into an altered Fc with substitution of Asp for Pro at position 238 according to EU numbering at sites predicted to affect the interaction with FcγRIIb (the residues of positions 233, 234, 235, 236, 237, 239, 240, 241, 263, 265, 266, 267, 268, 271, 273, 295, 296, 298, 300, 323, 325, 326, 327, 328, 330, 332, and 334 according to EU numbering); and interaction with each FcγR was evaluated.

[0468] The variable region of IL6R-H (SEQ ID NO: 17), which is the variable region of the antibody against the human interleukin 6 receptor disclosed in WO 2009/125825, was produced as the antibody H chain variable region, and IL6R-G1d (SEQ ID NO: 3) comprising G1d prepared by deleting the C-terminal Gly and Lys of human IgG1, was produced as the antibody H chain constant region. Next, the method of Reference Example 1 was followed for substituting Asp for Pro at position 238 in IL6R-G1d, according to EU numbering, to produce IL6R-F648. Two types of H chains were prepared as the template H chain for introducing comprehensive alterations: IL6R-F652 (SEQ ID NO: 1) produced by introducing M252Y and N434Y to IL6R-F648; and IL6R-BF648 (SEQ ID NO: 2) produced by introducing K439E to IL6R-F648. The above-mentioned site of IL6R-F652 or IL6R-BF648 was introduced with 18 types of amino acids excluding the original amino acid and Cys. IL6R-L (SEQ ID NO: 6) was utilized as a common antibody L chain, and together with the respective H chain, the antibodies were expressed and purified according to the method of Reference Example 1. These antibody variants were expressed and purified according to the method of Reference Example 1, and binding to each of the FcγRs (FcγRIa, FcγRIIa H type, FcγRIIa R type, FcγRIIb, FcγRIIIa V type) was comprehensively evaluated by the method of Reference Example 2.

[0469] As a result, L235F, G237Q, F241M, F241L, H268P, Q295M, Q295V, Y296E, Y296H, Y296N, Y296D, S298A, S298M, V323I, S324N, S324H, A330H, and A330Y were found to be alterations that lower binding to FcgRIIaR without greatly decreasing binding to FcgRIIb when combined with the P238D alteration (Tables 7 and 8).

[0470] Table 7 and Table 8 show respectively the relative FcgRIIaR- and FcgRIIb-binding activities of variants discovered by comprehensively introducing alterations into IL6R-F652/IL6R-L and into IL6R-BF648/IL6R-L. These values are obtained by dividing values for the amount of each variant bound to FcgRIIaR or FcgRIIb by the values for the amount of IL6R-F652/IL6R-L or IL6R-BF648/IL6R-L bound to the respective FcgR, and then multiplying these values by 100.

TABLE 7
		Relative
	Alterations	FcgRIIaR-	Relative
	introduced into	binding	FcgRIIb-
Variant Name	IL6R-F652	Activity	binding Activity
IL6R-F652/IL6R-L		100.0	100.0
IL6R-PD023/IL6R-L	A330H	73.9	101.5
IL6R-PD024/IL6R-L	A330Y	60.0	74.4
IL6R-PD089/IL6R-L	G237Q	65.0	66.0
IL6R-PD004/IL6R-L	H268P	77.0	80.5
IL6R-PD047/IL6R-L	L235F	89.9	95.4
IL6R-PD013/IL6R-L	S324H	90.8	91.9
IL6R-PD012/IL6R-L	S324N	92.5	102.2

TABLE 8
		Relative
	Alterations	FcgRIIaR-
	introduced	binding	Relative FcgRIIb-
Variant Name	into IL6R-BF648	Activity	binding Activity
IL6R-BF648/IL6R-L		100.0	100.0
IL6R-2B044/IL6R-L	F241L	55.7	65.5
IL6R-2B045/IL6R-L	F241M	64.7	81.1
IL6R-2B118/IL6R-L	Q295M	93.4	96.3
IL6R-2B124/IL6R-L	Q295V	65.2	72.9
IL6R-2B128/IL6R-L	Y296D	88.1	102.1
IL6R-2B129/IL6R-L	Y296E	68.4	93.3
IL6R-2B132/IL6R-L	Y296H	66.9	89.1
IL6R-2B137/IL6R-L	Y296N	77.7	98.3
IL6R-2B145/IL6R-L	S298A	45.1	55.5
IL6R-2B154/IL6R-L	S298M	77.5	79.4
IL6R-2B169/IL6R-L	V323I	90.8	102.9

[0471] From the results shown in Tables 7 and 8, all of these alterations were found to be those that lower FcgRIIaR binding while maintaining FcgRIIb binding to at least 55.5% or more in comparison to before introducing the alteration.

[0472] Therefore, the present investigation examined the production of variants whose FcgRIIaR binding is lowered as much as possible while FcgRIIb binding is maintained at a level similar to that of IgG1 by further combining these alterations. Specifically, alterations considered to selectively lower the binding to activating FcgRs in comparison to before introduction of the alterations in Tables 7 and 8, or combinations thereof were introduced into IL6R-F648. IL6R-L (SEQ ID NO: 6) was utilized as a common antibody L chain, and together with the respective H chain, the antibodies were expressed and purified according to the method of Reference Example 1. Binding of the obtained variants to FcgRIa, FcgRIIaR, FcgRIIaH, FcgRIIb, and FcgRIIIaV was evaluated according to the method of Reference Example 2. Table 9 shows the relative binding activities of each variant for FcgRIIaR and FcgRIIb. These are values obtained by dividing values for the amount of each variant bound to FcgRIIaR or FcgRIIb by values for the amount of IL6R-G1d/IL6R-L bound to FcgRIIaR or FcgRIIb, and then multiplying these values by 100.

TABLE 9
		Relative	Relative
		FcgRIIaR-	FcgRIIb-
		binding	binding
Variant Name	Alteration(s) introduced into G1d	Activity	Activity
IL6R-G1d/IL6R-L		100.0	100.0
IL6R-F648/IL6R-L	P238D	29.9	138.5
IL6R-P589/IL6R-L	P238D/Y296E	27.0	142.4
IL6R-P590/IL6R-L	P238D/F241M	20.6	126.2
IL6R-P591/IL6R-L	P238D/F241M/Y296E	20.0	131.2
IL6R-P594/IL6R-L	P238D/F241L	19.8	111.3
IL6R-P595/IL6R-L	P238D/Q295V	23.5	112.5
IL6R-P596/IL6R-L	P238D/Q295M	32.2	143.7
IL6R-P597/IL6R-L	P238D/Y296H	27.3	140.4
IL6R-P598/IL6R-L	P238D/Y296N	31.6	148.6
IL6R-P599/IL6R-L	P238D/V323I	30.9	148.9
IL6R-P600/IL6R-L	P238D/S298A	16.5	112.4
IL6R-P601/IL6R-L	P238D/S298M	27.3	131.7
IL6R-P602/IL6R-L	P238D/F241M/Q295V/Y296E	18.1	111.6
IL6R-P603/IL6R-L	P238D/F241M/Y296E/V323I	16.9	129.6
IL6R-P604/IL6R-L	P238D/F241M/Y296E/S298A	13.0	92.3
IL6R-P605/IL6R-L	P238D/Y296D/S298A	13.5	82.0
IL6R-P653/IL6R-L	P238D/F241M/H268P/Y296E	14.5	116.3
IL6R-P654/IL6R-L	P238D/F241M/Y296E/S324N	18.2	128.3
IL6R-P655/IL6R-L	P238D/F241M/Y296E/S324H	17.1	127.0
IL6R-P656/IL6R-L	P238D/F241M/Y296E/A330H	18.2	137.9
IL6R-P657/IL6R-L	P238D/F241M/Y296E/A330Y	15.4	115.0
IL6R-P658/IL6R-L	L235F/P238D/F241M/Y296E	20.8	149.2
IL6R-P659/IL6R-L	G237Q/P238D/F241M/Y296E	9.1	87.0
IL6R-P660/IL6R-L	P238D/S298A/S324N	14.7	100.4
IL6R-P661/IL6R-L	P238D/S298A/A330H	17.5	109.1
IL6R-P662/IL6R-L	P238D/Y296D/S298A/S324N	11.2	73.3
IL6R-P663/IL6R-L	P238D/Y296D/S298A/A330H	10.1	79.7
IL6R-P687/IL6R-L	P238D/S298A/S324N/A330H	12.6	100.2
IL6R-P688/IL6R-L	P238D/F241M/H268P/Y296E/S324N	13.3	113.7
IL6R-P689/IL6R-L	P238D/F241M/H268P/Y296E/S324H	10.1	102.9
IL6R-P690/IL6R-L	G237Q/P238D/F241M/Y296E/A330H	10.5	102.3
IL6R-P691/IL6R-L	L235F/G237Q/P238D/F241M/Y296E	10.7	103.6
IL6R-P692/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/A330H	13.7	126.9
IL6R-P693/IL6R-L	P238D/Y296E/S298A	11.4	87.9
IL6R-P718/IL6R-L	P238D/H268P	20.5	122.9
IL6R-P719/IL6R-L	P238D/S324N	24.0	0.2
IL6R-P720/IL6R-L	P238D/S324H	21.5	125.6
IL6R-P721/IL6R-L	P238D/A330H	24.1	140.2
IL6R-P722/IL6R-L	P238D/A330Y	22.5	130.0
IL6R-P723/IL6R-L	L235F/P238D	24.0	128.5
IL6R-P724/IL6R-L	G237Q/P238D	17.8	101.2
IL6R-P725/IL6R-L	P238D/Y296D	24.5	135.7
IL6R-P726/IL6R-L	G237Q/P238D/F241M/Y296E/S324H/A330H	12.0	123.0
IL6R-P727/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/A330H	8.3	107.9
IL6R-P728/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/S324H	11.0	126.0
IL6R-P729/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E	10.0	126.6
IL6R-P731/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/S324H	8.1	85.8
IL6R-P732/IL6R-L	L235F/P238D/F241M/H268P/Y296E/S324H	11.9	165.3
IL6R-P733/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/S324H	6.3	103.5
IL6R-P734/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/S324H/A330H	12.5	169.1
IL6R-P735/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/A330H	11.3	173.1
IL6R-P737/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/S324H/A330H	7.2	99.5

[0473] From among the variants shown in Table 9, variants whose FcgRIIaR binding has been lowered to 30% or less and FcgRIIb binding has been maintained at 80% or more in comparison to those of IL6R-G1d/IL6R-L are shown in Table 10 along with their KD values for each FcgR. Relative binding activity in the Table is a value obtained by dividing the KD value of IL6R-G 1 d/IL6R-L by the KD value of each variant, and represents the relative binding activity of each variant when the KD value of IL6R-G1d/IL6R-L for each FcgR is defined as 1. Among the KD values shown in the table, the values in the solid gray boxes are values calculated by utilizing Equation 2 of Reference Example 2, as the binding of FcgR to each variant is very weak and cannot be accurately analyzed by kinetic analysis.

KD=C·R_max/(R_eq−RI)−C  [Equation 2]

TABLE 10
		KD	KD	KD	KD
	Alteration(s) introduced	(M) for	(M) for	(M) for	(M) for
Variant Name	into IL6R-G1d	FcgRIa	FcgRIIaR	FcgRIIaH	FcgRIIb
IL6R-G1d/IL6R-L		3.3E−10	1.2E−06	9.6E−07	5.1E−06
IL6R-F648/IL6R-L	P238D	8.0E−09	1.9E−05	7.9E−05	1.9E−06
IL6R-P589/IL6R-L	P238D/Y296E	1.0E−08	2.7E−05	8.3E−05	1.8E−06
IL6R-P590/IL6R-L	P238D/F241M	1.7E−08	3.6E−05	9.1E−05	2.8E−06
IL6R-P591/IL6R-L	P238D/F241M/Y296E	2.4E−08	3.7E−05	8.6E−05	3.1E−06
IL6R-P594/IL6R-L	P238D/F241L	1.4E−08	3.9E−05	1.1E−04	2.9E−06
IL6R-P595/IL6R-L	P238D/Q295V	1.1E−08	3.2E−05	1.0E−04	2.2E−06
IL6R-P597/IL6R-L	P238D/Y296H	1.7E−08	2.6E−05	8.3E−05	2.0E−06
IL6R-P600/IL6R-L	P238D/S298A	3.5E−09	4.8E−05	1.4E−04	4.4E−06
IL6R-P601/IL6R-L	P238D/S298M	4.0E−09	2.6E−05	9.7E−05	2.6E−06
IL6R-P602/IL6R-L	P238D/F241M/Q295V/Y296E	1.9E−08	4.4E−05	1.0E−04	2.4E−06
IL6R-P603/IL6R-L	P238D/F241M/Y296E/V323I	2.2E−08	4.7E−05	1.1E−04	2.7E−06
IL6R-P604/IL6R-L	P238D/F241M/Y296E/S298A	1.3E−08	6.4E−05	1.2E−04	5.5E−06
IL6R-P605/IL6R-L	P238D/Y296D/S298A	8.6E−09	6.0E−05	1.3E−04	5.3E−06
IL6R-P653/IL6R-L	P238D/F241M/H268P/Y296E	1.8E−08	5.1E−05	1.1E−04	3.5E−06
IL6R-P654/IL6R-L	P238D/F241M/Y296E/S324N	1.6E−08	3.9E−05	6.8E−05	2.6E−06
IL6R-P655/IL6R-L	P238D/F241M/Y296E/S324H	1.8E−08	4.2E−05	5.8E−05	2.7E−06
IL6R-P656/IL6R-L	P238D/F241M/Y296E/A330H	2.8E−08	3.9E−05	8.4E−05	1.9E−06
IL6R-P657/IL6R-L	P238D/F241M/Y296E/A330Y	1.7E−08	4.7E−05	1.1E−04	3.1E−06
IL6R-P658/IL6R-L	L235F/P238D/F241M/Y296E	8.3E−07	3.3E−05	9.6E−05	1.4E−06
IL6R-P659/IL6R-L	G237Q/P238D/F241M/Y296E	8.0E−08	8.5E−05	1.6E−04	6.4E−06
IL6R-P660/IL6R-L	P238D/S298A/S324N	7.4E−09	5.0E−05	1.1E−04	5.3E−06
IL6R-P661/IL6R-L	P238D/S298A/A330H	1.0E−08	4.1E−05	9.4E−05	4.0E−06
IL6R-P687/IL6R-L	P238D/S298A/S324N/A330H	7.0E−09	8.1E−05	1.3E−04	5.1E−06
IL6R-P688/IL6R-L	P238D/F241M/H268P/Y296E/S324N	1.3E−08	5.8E−05	1.1E−04	4.1E−06
IL6R-P689/IL6R-L	P238D/F241M/H268P/Y296E/S324H	2.8E−08	7.8E−05	1.2E−04	4.7E−06
IL6R-P690/IL6R-L	G237Q/P238D/F241M/Y296E/A330H	3.1E−07	7.4E−05	1.4E−04	4.0E−06
IL6R-P691/IL6R-L	L235F/G237Q/P238D/F241M/Y296E	7.6E−07	7.2E−05	1.3E−04	4.3E−06
IL6R-P692/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/A330H	5.3E−07	5.6E−05	1.2E−04	2.7E−06
IL6R-P693/IL6R-L	P238D/Y296E/S298A	7.8E−09	6.8E−05	1.5E−04	5.6E−06
IL6R-P718/IL6R-L	P238D/H268P	1.3E−08	3.4E−05	1.1E−04	3.2E−06
IL6R-P719/IL6R-L	P238D/S324N	8.3E−09	2.8E−05	7.9E−05	2.2E−06
IL6R-P720/IL6R-L	P238D/S324H	9.7E−09	3.2E−05	7.3E−05	2.6E−06
IL6R-P721/IL6R-L	P238D/A330H	1.8E−08	2.8E−05	8.8E−05	1.8E−06
IL6R-P722/IL6R-L	P238D/A330Y	1.4E−08	3.0E−05	9.7E−05	2.9E−06
IL6R-P723/IL6R-L	L235F/P238D	8.6E−07	2.8E−05	8.5E−05	2.1E−06
IL6R-P724/IL6R-L	G237Q/P238D	3.3E−08	4.0E−05	1.2E−04	4.6E−06
IL6R-P725/IL6R-L	P238D/Y296D	1.6E−08	2.7E−05	9.0E−05	1.9E−06
IL6R-P726/IL6R-L	G237Q/P238D/F241M/Y296E/S324H/A330H	1.9E−07	6.6E−05	6.1E−05	4.1E−06
IL6R-P727/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/A330H	2.3E−07	1.0E−04	1.5E−04	4.8E−06
IL6R-P728/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/S324H	5.8E−07	7.4E−05	8.1E−05	3.9E−06
IL6R-P729/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E	6.6E−07	8.3E−05	1.8E−04	4.4E−06
IL6R-P731/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/S324H	6.8E−08	1.1E−04	1.5E−04	1.0E−05
IL6R-P732/IL6R-L	L235F/P238D/F241M/H268P/Y296E/S324H	7.0E−07	7.2E−05	1.0E−04	2.9E−06
IL6R-P733/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/S324H	2.5E−06	1.3E−04	1.8E−04	4.7E−06
IL6R-P734/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/S324H/A330H	2.2E−07	6.8E−05	8.4E−05	2.5E−06
IL6R-P735/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/A330H	2.6E−07	5.9E−05	1.4E−04	2.3E−06
IL6R-P737/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/S324H/A330H	1.7E−07	1.2E−04	1.6E−04	6.4E−06
			Relative	Relative	Relative	Relative	Relative
		KD	FcgRIa-	FcgRIIaR-	FcgRIIaH-	FcgRIIb-	FcgRIIIaV-
		(M) for	binding	binding	binding	binding	binding
	Variant Name	FcgRIIIaV	Activity	Activity	Activity	Activity	Activity
	IL6R-G1d/IL6R-L	3.4E−07	1.0000	1.000	1.000	1.000	1.000
	IL6R-F648/IL6R-L	6.9E−05	0.0413	0.065	0.012	2.749	0.005
	IL6R-P589/IL6R-L	4.1E−05	0.0330	0.046	0.012	2.856	0.008
	IL6R-P590/IL6R-L	6.6E−05	0.0194	0.034	0.011	1.836	0.005
	IL6R-P591/IL6R-L	4.4E−05	0.0138	0.034	0.011	1.647	0.008
	IL6R-P594/IL6R-L	6.4E−05	0.0236	0.032	0.009	1.766	0.005
	IL6R-P595/IL6R-L	7.1E−05	0.0300	0.039	0.010	2.315	0.005
	IL6R-P597/IL6R-L	4.5E−05	0.0194	0.048	0.012	2.636	0.008
	IL6R-P600/IL6R-L	6.1E−05	0.0943	0.026	0.007	1.163	0.006
	IL6R-P601/IL6R-L	5.4E−05	0.0825	0.048	0.010	2.000	0.006
	IL6R-P602/IL6R-L	6.1E−05	0.0174	0.028	0.010	2.133	0.006
	IL6R-P603/IL6R-L	8.3E−05	0.0150	0.026	0.009	1.897	0.004
	IL6R-P604/IL6R-L	4.0E−05	0.0254	0.019	0.008	0.938	0.008
	IL6R-P605/IL6R-L	4.4E−05	0.0384	0.021	0.007	0.977	0.008
	IL6R-P653/IL6R-L	5.0E−05	0.0183	0.024	0.009	1.456	0.007
	IL6R-P654/IL6R-L	4.8E−05	0.0206	0.032	0.014	2.016	0.007
	IL6R-P655/IL6R-L	4.6E−05	0.0183	0.030	0.017	1.890	0.007
	IL6R-P656/IL6R-L	5.0E−05	0.0118	0.032	0.011	2.677	0.007
	IL6R-P657/IL6R-L	6.5E−05	0.0194	0.026	0.009	1.637	0.005
	IL6R-P658/IL6R-L	7.1E−05	0.0004	0.038	0.010	3.807	0.005
	IL6R-P659/IL6R-L	7.3E−05	0.0041	0.015	0.006	0.807	0.005
	IL6R-P660/IL6R-L	3.0E−05	0.0446	0.025	0.009	0.972	0.011
	IL6R-P661/IL6R-L	2.9E−05	0.0330	0.030	0.010	1.285	0.012
	IL6R-P687/IL6R-L	4.2E−05	0.0471	0.020	0.007	1.006	0.008
	IL6R-P688/IL6R-L	4.4E−05	0.0254	0.021	0.009	1.269	0.008
	IL6R-P689/IL6R-L	5.2E−05	0.0118	0.016	0.008	1.091	0.007
	IL6R-P690/IL6R-L	6.3E−05	0.0011	0.017	0.007	1.279	0.005
	IL6R-P691/IL6R-L	4.8E−05	0.0004	0.017	0.007	1.187	0.007
	IL6R-P692/IL6R-L	5.7E−05	0.0006	0.022	0.008	1.890	0.006
	IL6R-P693/IL6R-L	4.0E−05	0.0423	0.018	0.006	0.918	0.008
	IL6R-P718/IL6R-L	4.2E−05	0.0254	0.036	0.009	1.616	0.008
	IL6R-P719/IL6R-L	5.6E−05	0.0398	0.044	0.012	2.358	0.006
	IL6R-P720/IL6R-L	6.4E−05	0.0340	0.039	0.013	2.008	0.005
	IL6R-P721/IL6R-L	6.2E−05	0.0183	0.044	0.011	2.840	0.006
	IL6R-P722/IL6R-L	4.9E−05	0.0236	0.041	0.010	1.760	0.007
	IL6R-P723/IL6R-L	3.9E−05	0.0004	0.044	0.011	2.448	0.009
	IL6R-P724/IL6R-L	4.7E−05	0.0100	0.031	0.008	1.130	0.007
	IL6R-P725/IL6R-L	4.5E−05	0.0206	0.046	0.011	2.749	0.008
	IL6R-P726/IL6R-L	3.6E−05	0.0017	0.019	0.016	1.251	0.009
	IL6R-P727/IL6R-L	6.6E−05	0.0014	0.012	0.007	1.064	0.005
	IL6R-P728/IL6R-L	5.0E−05	0.0006	0.017	0.012	1.311	0.007
	IL6R-P729/IL6R-L	5.6E−05	0.0005	0.015	0.005	1.166	0.006
	IL6R-P731/IL6R-L	5.3E−05	0.0049	0.012	0.007	0.499	0.006
	IL6R-P732/IL6R-L	7.6E−05	0.0005	0.017	0.010	1.748	0.004
	IL6R-P733/IL6R-L	7.7E−05	0.0001	0.009	0.006	1.089	0.004
	IL6R-P734/IL6R-L	1.0E−04	0.0015	0.018	0.011	2.073	0.003
	IL6R-P735/IL6R-L	7.3E−05	0.0013	0.021	0.007	2.264	0.005
	IL6R-P737/IL6R-L	6.8E−05	0.0020	0.011	0.006	0.802	0.005

[0474] Among the alterations introduced in this examination, it was shown that P589 produced by introducing Y296E, P590 produced by introducing F241M, P594 produced by introducing F241L, P595 produced by introducing Q295V, P597 produced by introducing Y296H, P600 produced by introducing S298A, P601 produced by introducing S298M, P718 produced by introducing H268P, P719 produced by introducing S324N, P720 produced by introducing S324H, P721 produced by introducing A330H, P722 produced by introducing A330Y, P723 produced by introducing L235F, P724 produced by introducing G237Q, and P725 produced by introducing Y296D have an effect of lowering FcgRIIaR binding in comparison to binding by F648 before introducing the alteration, while maintaining FcgRIIb binding to the same level as or stronger than the binding by G1d. Among these, P600 produced by introducing S298A had the most reduced FcgRIIaR binding. The FcgRIIaR-binding was lowered to 0.026-fold while FcgRIIb-binding was maintained at 1.2 times that of G1d.

[0475] As a result of examining combinations of alterations that showed an effect of selectively lowering binding to activating FcgRs, P727 was found to have 1.0-fold or higher FcgRIIb-binding in comparison to that of G1d, and the most reduced FcgRIIaR binding. In P727, the FcgRIIb binding was maintained at 1.1 times that of G1d, and the FcgRIIaR-binding was attenuated to 0.012 times. Furthermore, since its FcgRIa-binding was 0.0014 times that of G1d, FcgRIIaH-binding was attenuated to 0.007 times, and FcgRIIIaV binding was attenuated to 0.005 times, this was found to be an excellent variant having very selectively attenuated binding to activating FcgRs other than FcgRIIb.

(4-2) Examination of Lowering FcgRIIaR Binding of Variants with Enhanced FcgRIIb Binding

[0476] Next, variants whose FcgRIIb binding is maintained at a level similar to that of IgG1 while binding to other FcgRs is lowered in comparison to those of IgG1 were expected to be obtainable by introducing alterations that lower binding to all FcgRs including FcgRIIb into a template variant with selectively enhanced FcgRIIb binding but whose binding to other FcgRs has not been enhanced or has been lowered.

[0477] First, IL6R-B3 (SEQ ID NO: 4) was produced by introducing K439E into IL6R-G1d (SEQ ID NO: 3). Introducing FcgRIIb-selective binding in combination into IL6R-B3 produced variants with selectively enhanced FcgRIIb binding. IL6R-L (SEQ ID NO: 6) was utilized as a common antibody L chain, and together with the respective H chain, the antibodies were expressed and purified according to the method of Reference Example 1. Binding of the obtained variants to FcgRIa, FcgRIIaR, FcgRIIaH, FcgRIIb, and FcgRIIIaV was evaluated according the method of Reference Example 2. Binding activities of the produced variants for each FcgR are shown in Table 11. Relative binding activities shown in the table are values obtained by dividing the KD value of IL6R-B3/IL6R-L by the KD value of each variant, and represent the relative binding activities for each variant when the KD value of IL6R-B3/IL6R-L for each FcgR is defined as 1. “KD(IIaR)/KD(IIb)” is a value obtained by dividing the KD value of each variant for FcgRIIaR by the KD value of the variant for FcgRIIb; and the greater this value, the higher the selectivity for FcgRIIb. Among the KD values shown in the table, the values in the solid gray boxes are values calculated by utilizing Equation 2 of Reference Example 2, as the binding of FcgR to each variant is very weak and cannot be accurately analyzed by kinetic analysis.

KD=C·R_max/(R_eq−RI)−C  [Equation 2]

TABLE 11
								Relative	Relative
		KD	KD	KD	KD	KD		FcgRIIaR-	FcgRIIb-
	Alteration(s) introduced	(mol/L) for	(mol/L) for	(mol/L) for	(mol/L) for	(mol/L) for	KD(IIaR)/	binding	binding
Variant Name	into IL6R-B3	FcgRIa	FcgRIIaR	FcgRIIaH	FcgRIIb	FcgRIIIaV	KD(IIb)	Activity	Activity
IL6R-G1d/IL6R-L		3.2E−10	1.0E−06	6.7E−07	2.6E−06	3.5E−07	0.4	1.1	1.2
IL6R-B3/IL6R-L	*	4.2E−10	1.1E−06	7.7E−07	3.1E−06	3.3E−07	0.3	1.0	1.0
IL6R-BF648/IL6R-L	P238D	1.1E−08	1.5E−05	4.0E−05	1.2E−06	7.0E−05	13.0	0.1	2.6
IL6R-2B253/IL6R-L	P238D/V323M/E233D	1.4E−09	5.0E−06	1.3E−05	4.3E−07	5.0E−05	11.6	0.2	7.2
IL6R-2B261/IL6R-L	P238D/E233D/Y296D	9.0E−09	2.2E−05	3.3E−05	1.0E−06	7.3E−05	22.0	0.0	3.1
IL6R-BP082/IL6R-L	E233D/P238D/A330K	1.8E−08	1.2E−05	3.7E−05	5.4E−07	8.1E−05	22.8	0.1	5.8
IL6R-BP083/IL6R-L	P238D/Y296D/A330K	3.8E−08	2.3E−05	4.4E−05	7.9E−07	6.6E−05	29.0	0.0	3.9
IL6R-BP084/IL6R-L	P238D/V323M/A330K	7.0E−09	7.2E−06	2.4E−05	5.0E−07	6.7E−05	14.3	0.1	6.1
IL6R-BP085/IL6R-L	G237D/P238D/A330K	2.9E−07	4.2E−06	2.4E−05	3.2E−07	6.8E−05	13.1	0.3	9.6
IL6R-BP086/IL6R-L	P238D/K326A/A330K	2.7E−08	9.7E−06	3.4E−05	5.7E−07	5.7E−05	17.1	0.1	5.4
IL6R-BP087/IL6R-L	L234Y/P238D/A330K	3.8E−08	9.7E−06	2.1E−05	6.1E−07	4.4E−05	16.0	0.1	5.1
IL6R-BP088/IL6R-L	G237D/P238D/K326A/A330K	3.9E−07	2.9E−06	2.3E−05	2.2E−07	5.7E−05	13.3	0.4	14.3
IL6R-BP089/IL6R-L	L234Y/P238D/K326A/A330K	6.3E−08	6.4E−06	2.0E−05	3.9E−07	5.1E−05	16.6	0.2	8.0
IL6R-BP129/IL6R-L	E233D/P238D/Y296D/A330K	2.5E−08	1.5E−05	4.0E−05	5.2E−07	7.5E−05	29.3	0.1	6.0
IL6R-BP130/IL6R-L	E233D/P238D/V323M/A330K	1.8E−09	5.3E−06	2.6E−05	3.0E−07	7.1E−05	17.5	0.2	10.2
IL6R-BP131/IL6R-L	E233D/G237D/P238D/A330K	1.2E−07	3.1E−06	1.4E−05	2.5E−07	5.9E−05	12.5	0.4	12.6
IL6R-BP132/IL6R-L	E233D/P238D/K326A/A330K	1.5E−08	8.0E−06	3.0E−05	3.7E−07	5.8E−05	21.5	0.1	8.4
IL6R-BP133/IL6R-L	E233D/L234Y/P238D/A330K	1.3E−07	8.6E−06	2.6E−05	5.6E−07	6.2E−05	15.5	0.1	5.6
IL6R-BP143/IL6R-L	L234Y/P238D/K326A	1.6E−08	5.7E−06	2.7E−05	5.7E−07	6.2E−05	10.0	0.2	5.4
IL6R-BP144/IL6R-L	G237D/P238D/K326A	3.7E−08	6.9E−06	3.6E−05	7.9E−07	5.4E−05	8.7	0.2	3.9
IL6R-BP145/IL6R-L	L234Y/G237D/P238D	1.2E−07	3.4E−06	1.7E−05	3.4E−07	4.5E−05	9.9	0.3	9.1
IL6R-BP146/IL6R-L	L234Y/G237D/P238D/K326A	7.4E−08	2.1E−06	1.8E−05	2.3E−07	3.8E−05	9.3	0.5	13.7
IL6R-BP147/IL6R-L	L234Y/G237D/P238D/K326A/A330K	1.4E−07	8.9E−07	5.1E−06	6.6E−08	3.3E−05	13.6	1.2	47.1
IL6R-BP148/IL6R-L	E233D/L234Y/G237D/P238D/K326A/A330K	8.9E−08	1.1E−06	7.0E−06	7.5E−08	3.0E−05	14.5	1.0	41.4
IL6R-BP149/IL6R-L	E233D/L234Y/G237D/P238D/Y296D/K326A/A330K	1.2E−07	1.4E−06	8.4E−06	9.3E−08	3.7E−05	15.0	0.8	33.1
IL6R-BP150/IL6R-L	L234Y/G237D/P238D/K326A/A330R	3.2E−07	5.5E−07	3.4E−06	3.1E−08	3.4E−05	17.7	2.0	99.0
IL6R-BP151/IL6R-L	E233D/L234Y/G237D/P238D/K326A/A330R	8.4E−08	6.7E−07	4.7E−06	4.0E−08	3.1E−05	16.9	1.6	77.4
IL6R-BP152/IL6R-L	E233D/L234Y/G237D/P238D/Y296D/K326A/A330R	7.3E−08	8.1E−07	5.6E−06	4.1E−08	4.8E−05	19.5	1.3	75.0
IL6R-BP176/IL6R-L	E233D/P238D/K326D/A330K	7.3E−09	6.9E−06	3.0E−05	3.6E−07	5.3E−05	19.1	0.2	8.6
IL6R-BP177/IL6R-L	E233D/L234Y/G237D/P238D/P271G/K326D/A330K	3.3E−08	7.1E−07	8.2E−06	5.2E−08	3.7E−05	13.8	1.5	60.0
IL6R-BP178/IL6R-L	E233D/G237D/P238D/P271G/A330K	4.3E−08	9.3E−07	1.4E−05	5.1E−08	6.4E−05	18.1	1.2	60.1
IL6R-BP179/IL6R-L	G237D/P238D/P271G/K326A/A330K	6.4E−08	1.4E−06	1.6E−05	8.4E−08	5.6E−05	16.7	0.8	36.9
IL6R-BP180/IL6R-L	G237D/P238D/P271G/A330K	9.8E−08	1.2E−06	1.8E−05	6.2E−08	7.0E−05	18.6	0.9	49.9
IL6R-BP181/IL6R-L	E233D/P238D/P271G/K326A/A330K	7.5E−09	3.2E−06	2.6E−05	1.6E−07	5.7E−05	20.3	0.3	19.3
IL6R-BP182/IL6R-L	E233D/P238D/P271G/Y296D/A330K	1.0E−08	2.6E−06	2.8E−05	1.1E−07	5.8E−05	23.5	0.4	28.3
IL6R-BP183/IL6R-L	E233D/L234Y/P238D/P271G/K326A/A330K	1.7E−08	2.6E−06	1.5E−05	2.4E−07	5.6E−05	10.8	0.4	12.9
IL6R-BP184/IL6R-L	E233D/P238D/P271G/A330K	1.1E−08	2.3E−06	3.0E−05	1.3E−07	6.6E−05	18.2	0.5	24.5
IL6R-BP185/IL6R-L	E233D/L234Y/G237D/P238D/P271G/K326A/A330K	6.3E−08	8.8E−07	7.3E−06	6.9E−08	3.6E−05	12.6	1.2	44.5
IL6R-BP186/IL6R-L	E233D/L234Y/G237D/P238D/P271G/Y296D/K326A/A330K	4.5E−08	9.6E−07	9.3E−06	6.1E−08	4.9E−05	15.8	1.1	50.7
IL6R-BP187/IL6R-L	L234Y/P238D/P271G/K326A/A330K	2.5E−08	2.8E−06	1.8E−05	2.9E−07	5.6E−05	9.7	0.4	10.7
IL6R-BP188/IL6R-L	E233D/G237D/P238D/H268D/P271G/A330K	2.1E−08	1.0E−06	1.6E−05	4.6E−08	5.8E−05	21.9	1.1	67.6
IL6R-BP189/IL6R-L	G237D/P238D/H268D/P271G/K326A/A330K	4.2E−08	1.4E−06	2.1E−05	7.4E−08	4.9E−05	18.5	0.8	41.8
IL6R-BP190/IL6R-L	G237D/P238D/H268D/P271G/A330K	6.3E−08	1.1E−06	1.7E−05	5.8E−08	4.5E−05	19.3	1.0	53.2
IL6R-BP191/IL6R-L	E233D/P238D/H268D/P271G/K326A/A330K	4.0E−09	3.0E−06	2.7E−05	1.5E−07	4.9E−05	20.3	0.4	21.2
IL6R-BP192/IL6R-L	E233D/P238D/H268D/P271G/Y296D/A330K	6.6E−09	2.6E−06	3.2E−05	1.1E−07	5.9E−05	23.1	0.4	27.3
IL6R-BP193/IL6R-L	E233D/P238D/H268D/P271G/A330K	6.3E−09	2.2E−06	2.5E−05	1.2E−07	5.1E−05	18.3	0.5	25.5
IL6R-BP194/IL6R-L	E233D/L234Y/G237D/P238D/H268D/P271G/K326A/A330K	2.4E−08	8.2E−07	8.5E−06	5.2E−08	2.7E−05	15.8	1.3	59.4
IL6R-BP195/IL6R-L	E233D/L234Y/G237D/P238D/H268D/P271G/Y296D/K326A/A330K	2.3E−08	9.1E−07	1.0E−05	5.0E−08	3.1E−05	18.2	1.2	62.0
IL6R-BP196/IL6R-L	L234Y/P238D/H268D/P271G/K326A/A330K	1.4E−08	3.0E−06	1.9E−05	2.2E−07	5.1E−05	13.4	0.4	13.9
IL6R-BP197/IL6R-L	E233D/L234Y/G237D/P238D/H268D/P271G/Y296D/K326D/A330K	1.9E−08	9.8E−07	1.2E−05	5.8E−08	3.3E−05	17.1	1.1	53.7
IL6R-BP198/IL6R-L	E233D/L234Y/P238D/H268D/P271G/K326A/A330K	1.1E−08	2.2E−06	2.0E−05	2.0E−07	4.4E−05	11.0	0.5	15.7
IL6R-BP199/IL6R-L	E233D/P238D/K326D/A330R	6.4E−09	8.6E−06	2.6E−05	4.9E−07	6.1E−05	17.6	0.1	6.3
IL6R-BP200/IL6R-L	E233D/L234Y/G237D/P238D/P271G/K326D/A330R	3.3E−08	6.3E−07	4.2E−06	3.4E−08	3.8E−05	18.5	1.7	90.9
IL6R-BP201/IL6R-L	E233D/G237D/P238D/P271G/A330R	5.1E−08	8.4E−07	6.9E−06	4.0E−08	5.2E−05	21.0	1.3	77.3
IL6R-BP202/IL6R-L	G237D/P238D/P271G/K326A/A330R	9.5E−08	1.2E−06	9.2E−06	6.4E−08	5.9E−05	18.8	0.9	48.3
IL6R-BP203/IL6R-L	G237D/P238D/P271G/A330R	1.8E−07	9.9E−07	1.1E−05	4.9E−08	7.2E−05	20.2	1.1	63.1
IL6R-BP204/IL6R-L	E233D/P238D/P271G/K326A/A330R	7.6E−09	4.5E−06	2.1E−05	2.5E−07	5.2E−05	18.0	0.2	12.4
IL6R-BP205/IL6R-L	E233D/P238D/P271G/Y296D/A330R	7.7E−09	3.5E−06	2.8E−05	1.6E−07	6.8E−05	21.9	0.3	19.3
IL6R-BP206/IL6R-L	E233D/P238D/P271G/A330R	8.2E−09	3.1E−06	2.4E−05	2.0E−07	6.9E−05	15.5	0.3	15.5
IL6R-BP207/IL6R-L	E233D/P238D/A330R	2.2E−08	1.9E−05	2.9E−05	8.4E−07	6.5E−05	22.6	0.1	3.7
IL6R-BP208/IL6R-L	E233D/G237D/P238D/H268D/P271G/A330R	1.9E−08	8.5E−07	8.3E−06	3.2E−08	5.3E−05	26.6	1.3	96.6
IL6R-BP209/IL6R-L	G237D/P238D/H268D/P271G/K326A/A330R	3.9E−08	1.2E−06	1.0E−05	5.1E−08	4.1E−05	23.5	0.9	60.6
IL6R-BP210/IL6R-L	G237D/P238D/H268D/P271G/A330R	6.5E−08	1.0E−06	9.5E−06	3.9E−08	4.6E−05	25.6	1.1	79.2
IL6R-BP211/IL6R-L	E233D/P238D/H268D/P271G/K326A/A330R	4.2E−09	4.1E−06	2.7E−05	2.2E−07	7.3E−05	18.6	0.3	14.0
IL6R-BP212/IL6R-L	E233D/P238D/H268D/P271G/Y296D/A330R	5.2E−09	3.5E−06	2.2E−05	1.7E−07	5.2E−05	20.6	0.3	18.2
IL6R-BP213/IL6R-L	E233D/P238D/H268D/P271G/A330R	4.1E−09	3.1E−06	2.4E−05	1.8E−07	6.3E−05	17.2	0.3	17.2
IL6R-BP214/IL6R-L	E233D/L234Y/G237D/P238D/Y296D/K326D/A330K	5.9E−08	1.7E−06	9.2E−06	1.2E−07	3.8E−05	14.2	0.6	25.8
IL6R-BP215/IL6R-L	G237D/P238D/H268D/P271G/Y296D/A330K	4.3E−08	1.3E−06	1.4E−05	4.1E−08	6.7E−05	31.7	0.8	75.4
IL6R-BP216/IL6R-L	G237D/P238D/S267Q/H268D/P271G/A330K	6.2E−07	2.9E−06	2.6E−05	1.4E−07	5.3E−05	20.7	0.4	22.1
IL6R-BP217/IL6R-L	G237D/P238D/S267Q/H268D/P271G/Y296D/A330K	2.8E−06	3.6E−06	2.8E−05	1.5E−07	6.0E−05	24.0	0.3	20.6
IL6R-BP218/IL6R-L	G237D/P238D/H268D/P271G/K326D/A330K	3.7E−08	1.5E−06	1.2E−05	7.6E−08	3.8E−05	19.7	0.7	40.7
IL6R-BP219/IL6R-L	L234Y/G237D/P238D/H268D/P271G/A330K	4.6E−08	6.1E−07	2.5E−06	3.4E−08	2.9E−05	17.9	1.8	90.9
IL6R-BP220/IL6R-L	E233D/G237D/P238D/H268D/P271G/Y296D/A330K	2.0E−08	1.1E−06	1.2E−05	3.6E−08	5.8E−05	30.6	1.0	85.8
IL6R-BP221/IL6R-L	L234Y/G237D/P238D/Y296D/K326A/A330R	1.3E−07	7.1E−07	2.5E−06	2.8E−08	4.6E−05	25.4	1.5	110.4
IL6R-BP222/IL6R-L	L234Y/G237D/P238D/P271G/K326A/A330R	5.1E−08	7.1E−07	2.6E−06	3.4E−08	4.7E−05	20.9	1.5	90.9
IL6R-BP223/IL6R-L	L234Y/G237D/P238D/H268D/P271G/K326A/A330R	2.7E−08	6.0E−07	2.8E−06	2.5E−08	3.2E−05	24.0	1.8	123.6
IL6R-BP224/IL6R-L	L234Y/G237D/P238D/S267Q/H268D/P271G/K326A/A330R	6.2E−09	4.5E−07	9.5E−06	3.5E−08	4.1E−05	12.9	2.4	88.3
IL6R-BP225/IL6R-L	L234Y/G237D/P238D/K326D/A330R	9.5E−08	6.9E−07	2.8E−06	3.5E−08	3.2E−05	19.7	1.6	88.3
IL6R-BP226/IL6R-L	L234Y/G237D/P238D/P271G/K326D/A330R	5.2E−08	5.7E−07	2.4E−06	3.3E−08	3.6E−05	17.3	1.9	93.6
IL6R-BP227/IL6R-L	L234Y/G237D/P238D/H268D/P271G/K326D/A330R	2.7E−08	6.2E−07	2.9E−06	3.2E−08	2.6E−05	19.4	1.7	96.6
IL6R-BP228/IL6R-L	L234Y/G237D/P238D/S267Q/H268D/P271G/K326D/A330R	5.5E−09	4.2E−07	1.1E−05	4.0E−08	3.2E−05	10.5	2.6	77.3
IL6R-BP229/IL6R-L	E233D/L234Y/G237D/P238D/P271G/K326A/A330R	5.6E−08	8.1E−07	3.3E−06	4.2E−08	3.7E−05	19.3	1.3	73.6
IL6R-BP230/IL6R-L	E233D/G237D/P238D/H268D/P271G/Y296D/A330R	1.4E−08	5.7E−07	9.6E−06	2.1E−08	6.7E−05	27.1	1.9	147.1
IL6R-BP231/IL6R-L	G237D/P238D/H268D/P271G/Y296D/A330R	9.4E−09	7.4E−07	1.1E−05	2.3E−08	4.0E−05	32.2	1.5	134.3
IL6R-BP232/IL6R-L	L234Y/G237D/P238D/P271G/K326A/A330K	7.6E−08	8.4E−07	3.3E−06	5.6E−08	4.5E−05	15.0	1.3	55.2
IL6R-BP233/IL6R-L	L234Y/G237D/P238D/P271G/A330K	7.0E−08	6.9E−07	2.8E−06	3.7E−08	5.1E−05	18.6	1.6	83.5
IL6R-BP234/IL6R-L	E233D/L234Y/G237D/P238D/S267Q/H268D/P271G/Y296D/K326D/A330K	6.5E−09	1.2E−06	2.0E−05	1.2E−07	3.1E−05	10.0	0.9	25.8
IL6R-BP235/IL6R-L	E233D/L234Y/G237D/P238D/H268D/P271G/Y296D/K326D/A330R	3.5E−09	6.8E−07	7.5E−06	4.4E−08	2.5E−05	15.5	1.6	70.2
IL6R-BP236/IL6R-L	E233D/L234Y/G237D/P238D/S267Q/H268D/P271G/Y296D/K326D/A330R	7.7E−09	8.4E−07	1.9E−05	6.5E−08	3.9E−05	12.9	1.3	47.5
IL6R-BP237/IL6R-L	E233D/L234Y/G237D/P238D/S267Q/H268D/P271G/Y296D/K326A/A330K	4.1E−09	1.1E−06	1.9E−05	1.0E−07	3.5E−05	11.0	1.0	30.9
IL6R-BP238/IL6R-L	E233D/L234Y/G237D/P238D/H268D/P271G/Y296D/K326A/A330R	2.4E−09	6.4E−07	7.0E−06	3.6E−08	2.7E−05	17.8	1.7	85.8
IL6R-BP239/IL6R-L	E233D/L234Y/G237D/P238D/S267Q/H268D/P271G/Y296D/K326A/A330R	7.6E−09	8.1E−07	1.7E−05	6.0E−08	4.8E−05	13.5	1.3	51.5
IL6R-BP240/IL6R-L	E233D/G237D/P238D/S267Q/H268D/P271G/A330R	7.6E−09	1.5E−06	2.6E−05	9.5E−08	5.2E−05	15.8	0.7	32.5
IL6R-BP241/IL6R-L	E233D/G237D/P238D/H268D/P271G/K326D/A330R	1.1E−09	6.8E−07	9.0E−06	4.5E−08	3.1E−05	15.1	1.6	68.7
IL6R-BP242/IL6R-L	E233D/G237D/P238D/H268D/P271G/K326A/A330R	1.9E−09	7.5E−07	8.6E−06	5.1E−08	2.9E−05	14.7	1.4	60.6
IL6R-BP243/IL6R-L	E233D/L234Y/G237D/P238D/H268D/P271G/A330R	3.0E−09	5.4E−07	6.0E−06	3.6E−08	2.5E−05	15.0	2.0	85.8
IL6R-BP244/IL6R-L	E233D/G237D/P238D/S267Q/H268D/P271G/Y296D/A330R	7.8E−09	1.8E−06	2.6E−05	1.1E−07	4.6E−05	16.4	0.6	28.1
IL6R-BP245/IL6R-L	E233D/G237D/P238D/S267Q/H268D/P271G/Y296D/K326D/A330R	6.3E−09	1.4E−06	2.5E−05	8.3E−08	3.9E−05	16.9	0.8	37.2
IL6R-BP246/IL6R-L	E233D/G237D/P238D/S267Q/H268D/P271G/Y296D/K326A/A330R	8.0E−09	1.6E−06	2.3E−05	9.2E−08	4.4E−05	17.4	0.7	33.6
IL6R-BP247/IL6R-L	E233D/G237D/P238D/H268D/P271G/Y296D/K326D/A330R	7.5E−09	8.1E−07	1.2E−05	3.7E−08	4.4E−05	21.9	1.3	83.5
IL6R-BP248/IL6R-L	E233D/G237D/P238D/H268D/P271G/Y296D/K326A/A330R	1.7E−09	8.2E−07	1.1E−05	3.5E−08	4.4E−05	23.4	1.3	88.3
IL6R-BP249/IL6R-L	E233D/L234Y/G237D/P238D/H268D/P271G/Y296D/A330R	7.0E−09	6.2E−07	7.2E−06	3.7E−08	2.8E−05	16.8	1.7	83.5
IL6R-BP262/IL6R-L	G237D/P238D/H268E/P271G	1.0E−08	2.0E−06	4.5E−05	1.2E−07	5.6E−05	17.0	0.5	25.8
IL6R-BP264/IL6R-L	E233D/G237D/P238D/H268E/P271G/Y296D/A330R	7.4E−09	3.5E−07	2.8E−06	1.2E−08	2.6E−05	28.3	3.1	251.2
IL6R-BP265/IL6R-L	G237D/P238D/H268E/P271G/Y296D/A330R	2.3E−08	6.3E−07	1.2E−05	1.5E−08	9.6E−05	41.2	1.7	203.3
IL6R-BP266/IL6R-L	E233D/G237D/P238D/H268E/P271G/A330R	1.4E−08	3.2E−07	1.1E−05	1.8E−08	4.0E−05	18.0	3.4	174.6
IL6R-BP268/IL6R-L	E233D/G237D/P238D/H268E/P271G/Y296D	4.5E−09	1.8E−06	2.1E−05	9.2E−08	2.8E−05	19.6	0.6	33.6
IL6R-BP269/IL6R-L	G237D/P238D/H268E/P271G/Y296D	1.4E−08	2.2E−06	7.2E−05	1.1E−07	1.4E−04	19.6	0.5	27.3
IL6R-BP423/IL6R-L	E233D/G237D/P238D/S267A/H268E/P271G/A330R	7.7E−10	1.8E−07	2.0E−06	5.1E−09	1.6E−05	34.2	6.2	603.5
IL6R-BP425/IL6R-L	E233D/G237D/P238D/V266L/S267A/H268E/P271G/A330R	4.1E−09	2.2E−07	1.5E−05	9.1E−09	4.2E−05	23.6	5.0	338.8
IL6R-BP426/IL6R-L	E233D/G237D/P238D/S267A/H268E/E269D/P271G/A330R	1.0E−09	1.6E−07	4.9E−05	5.9E−09	4.1E−05	27.6	6.7	527.3
IL6R-BP428/IL6R-L	E233D/G237D/P238D/S267G/H268E/P271G/A330R	4.9E−09	3.9E−07	4.2E−05	1.4E−08	3.6E−05	28.0	2.8	220.7
IL6R-BP429/IL6R-L	E233D/G237D/P238D/V264I/S267G/H268E/P271G/A330R	6.2E−09	1.7E−07	3.5E−06	5.4E−09	6.8E−05	31.5	6.4	572.2
IL6R-BP430/IL6R-L	E233D/G237D/P238D/V266L/S267G/H268E/P271G/A330R	1.7E−08	2.2E−07	1.1E−05	1.2E−08	3.5E−05	18.5	4.8	255.4
IL6R-BP431/IL6R-L	E233D/G237D/P238D/S267G/H268E/E269D/P271G/A330R	3.6E−09	4.1E−07	7.6E−06	1.2E−08	3.2E−05	34.6	2.7	264.1
IL6R-BP433/IL6R-L	E233D/G237D/P238D/H268D/P271G/Y296D/A330K/I332T	7.5E−10	6.8E−07	7.3E−06	3.4E−08	2.6E−05	20.0	1.6	91.4
IL6R-BP434/IL6R-L	E233D/G237D/P238D/H268D/P271G/Y296D/K326D/A330R/I332T	5.5E−10	3.4E−07	4.1E−06	1.2E−08	2.5E−05	27.2	3.2	251.2
IL6R-BP435/IL6R-L	E233D/G237D/P238D/H268D/P271G/Y296D/K326A/A330R/I332T	1.0E−09	4.2E−07	3.4E−06	1.6E−08	2.2E−05	27.1	2.6	198.1
IL6R-BP436/IL6R-L	E233D/G237D/P238D/S267A/H268E/P271G/Y296D/A330R/I332T	2.6E−10	2.2E−07	2.1E−06	5.1E−09	1.3E−05	43.8	4.8	604.7
IL6R-BP437/IL6R-L	G237D/P238D/S267A/H268E/P271G/Y296D/A330R/I332T	7.5E−10	2.2E−07	1.4E−06	5.9E−09	1.1E−05	37.7	4.8	522.0
IL6R-BP438/IL6R-L	E233D/G237D/P238D/S267A/H268E/P271G/A330R/I332T	2.1E−10	1.8E−07	1.6E−06	5.5E−09	6.8E−06	32.7	6.1	567.0
IL6R-BP439/IL6R-L	E233D/G237D/P238D/V264I/V266L/S267A/H268E/P271G/A330R	8.7E−09	1.3E−07	2.8E−06	6.1E−09	6.6E−05	20.9	8.5	508.2
IL6R-BP440/IL6R-L	E233D/G237D/P238D/V264I/H268E/P271G/A330R	8.7E−09	1.3E−07	1.6E−06	5.2E−09	2.8E−05	24.0	8.6	593.1
IL6R-BP441/IL6R-L	E233D/G237D/P238D/V266L/H268E/P271G/A330R	1.7E−08	3.6E−07	8.8E−06	1.5E−08	3.7E−05	24.0	3.0	204.6
IL6R-BP442/IL6R-L	E233D/G237D/P238D/H268E/E269D/P271G/A330R	4.5E−09	3.8E−07	4.7E−06	1.2E−08	2.5E−05	30.6	2.8	249.2
IL6R-BP443/IL6R-L	E233D/G237D/P238D/V266L/H268E/E269D/P271G/A330R	1.8E−08	5.1E−07	9.5E−06	2.3E−08	2.3E−05	21.7	2.1	132.1
IL6R-BP445/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/A330R	2.0E−09	8.0E−08	1.5E−06	2.6E−09	2.4E−05	31.0	13.5	1197.7
IL6R-BP479/IL6R-L	E233D/G237D/P238D/V264I/V266L/S267A/H268E/P271G	5.3E−09	9.0E−07	1.5E−05	5.6E−08	4.0E−05	16.1	1.2	55.3
IL6R-BP480/IL6R-L	E233D/G237D/P238D/V266L/H268E/E269D/P271G	1.3E−08	6.3E−06	2.1E−05	2.0E−07	4.6E−05	32.1	0.2	15.8
IL6R-BP481/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G	1.0E−09	4.0E−07	6.8E−06	1.9E−08	2.4E−05	20.5	2.7	159.3
IL6R-BP483/IL6R-L	E233D/G237D/P238D/V266L/S267A/H268E/P271G	1.3E−09	1.3E−06	1.8E−05	7.8E−08	2.5E−05	16.8	0.8	39.6
IL6R-BP484/IL6R-L	E233D/G237D/P238D/S267A/H268E/E269D/P271G	8.2E−10	7.8E−07	1.1E−05	4.6E−08	2.5E−05	17.1	1.4	67.6
IL6R-BP487/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/A330R/P396M	1.2E−09	3.9E−08	8.4E−07	1.2E−09	1.0E−05	33.8	27.8	2687.0
IL6R-BP488/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R	2.2E−09	7.4E−08	1.6E−06	1.9E−09	2.0E−05	40.1	14.6	1670.3
IL6R-BP489/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	1.3E−09	4.3E−08	8.7E−07	1.0E−09	1.2E−05	42.8	25.2	3090.0
IL6R-BP490/IL6R-L	G237D/P238D/V264I/S267A/H268E/P271G/A330R	4.5E−09	1.1E−07	2.4E−06	2.4E−09	2.3E−05	46.7	9.6	1287.5
IL6R-BP491/IL6R-L	G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R	5.3E−09	1.2E−07	2.2E−06	3.0E−09	2.1E−05	38.8	9.2	1016.4
IL6R-BP492/IL6R-L	P238D/V264I/S267A/H268E/P271G	7.9E−10	9.2E−07	1.6E−05	2.4E−08	3.6E−05	38.8	1.2	130.9
IL6R-BP493/IL6R-L	P238D/V264I/S267A/H268E/P271G/Y296D	8.2E−10	1.1E−06	1.9E−05	2.1E−08	3.5E−05	52.1	1.0	146.4
IL6R-BP494/IL6R-L	G237D/P238D/S267A/H268E/P271G/Y296D/A330R	3.9E−09	2.5E−07	5.4E−06	6.6E−09	4.0E−05	38.6	4.3	469.6
IL6R-BP495/IL6R-L	G237D/P238D/S267G/H268E/P271G/Y296D/A330R	8.3E−09	4.9E−07	1.2E−05	9.7E−09	3.3E−05	50.9	2.2	320.2
IL6R-BP496/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/Y296D	1.2E−09	4.7E−07	3.7E−06	1.8E−08	3.0E−05	25.5	2.3	167.9
IL6R-BP497/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/A327G/A330R	2.1E−09	8.5E−08	9.6E−07	4.1E−09	2.8E−05	21.0	12.6	761.1
IL6R-BP498/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/A330R/P396L	1.3E−09	5.1E−08	9.3E−07	1.7E−09	1.0E−05	30.8	21.3	1872.7
IL6R-BP499/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396L	1.2E−09	4.9E−08	1.0E−06	1.5E−09	1.2E−05	33.8	21.9	2116.4
IL6R-BP500/IL6R-L	G237D/P238D/V264I/S267A/H268E/P271G/Y296D	2.3E−09	7.2E−07	2.5E−05	2.4E−08	3.9E−05	29.9	1.5	127.7
IL6R-BP501/IL6R-L	G237D/P238D/V264I/S267A/H268E/P271G	2.1E−09	6.3E−07	1.4E−05	2.5E−08	1.9E−05	25.1	1.7	122.6
IL6R-BP502/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A327G/A330R	2.1E−09	1.1E−07	1.3E−06	3.7E−09	2.4E−05	29.5	9.9	837.4
IL6R-BP503/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A327G/A330R/P396M	1.2E−09	5.7E−08	8.6E−07	1.7E−09	2.1E−05	33.2	19.0	1807.0
IL6R-BP504/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/E272P	1.4E−09	4.5E−07	1.6E−05	2.4E−08	3.4E−05	18.5	2.4	127.2
IL6R-BP505/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/E272D	1.1E−09	4.3E−07	1.1E−05	2.1E−08	3.8E−05	20.0	2.5	144.4
IL6R-BP506/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/E272P/Y296D/A330R	3.1E−09	1.2E−07	2.5E−06	3.4E−09	5.5E−05	35.1	9.0	903.5
IL6R-BP507/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/E272P/A330R	2.6E−09	1.0E−07	1.8E−06	2.9E−09	2.6E−05	34.2	10.9	1061.9
IL6R-BP508/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/E272P/Y296D	1.4E−09	5.4E−07	2.0E−05	2.1E−08	6.1E−05	26.0	2.0	148.6
IL6R-BP509/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/E272D/Y296D	1.1E−09	5.2E−07	7.9E−06	1.8E−08	2.3E−05	29.2	2.1	173.6
IL6R-BP510/IL6R-L	G237D/P238D/V264I/S267A/H268E/P271G/E272P/A330R	6.0E−09	1.7E−07	4.0E−06	3.8E−09	2.5E−05	43.5	6.5	804.7
IL6R-BP511/IL6R-L	G237D/P238D/V264I/S267A/H268E/P271G/E272P/Y296D/A330R	6.0E−09	1.8E−07	4.3E−06	3.5E−09	7.1E−05	50.6	6.1	887.9
IL6R-BP531/IL6R-L	E233D/G237D/P238D/V264I/S267G/H268E/P271G/Y296D/A330R/P396M	9.4E−09	1.2E−07	3.5E−06	3.8E−09	2.7E−05	33.1	8.7	824.0
IL6R-BP532/IL6R-L	E233D/G237D/P238D/V264I/H268E/P271G/Y296D/A330R/P396M	1.2E−08	9.4E−08	1.9E−06	3.2E−09	2.6E−05	29.3	11.5	965.6
IL6R-BP533/IL6R-L	E233D/G237D/P238D/V264I/S267G/H268E/P271G/Y296D/A330R/P396L	7.7E−09	1.2E−07	2.6E−06	4.1E−09	2.7E−05	29.3	9.0	753.7
IL6R-BP534/IL6R-L	E233D/G237D/P238D/V264I/H268E/P271G/Y296D/A330R/P396L	9.3E−09	9.1E−08	1.8E−06	3.0E−09	2.5E−05	30.7	11.8	1040.4
IL6R-BP535/IL6R-L	E233D/G237D/P238D/V264I/S267G/H268E/P271G/Y296D/A327G/A330R/P396M	1.1E−08	9.2E−08	3.2E−06	4.0E−09	3.3E−05	23.2	11.7	778.3
IL6R-BP536/IL6R-L	E233D/G237D/P238D/V264I/H268E/P271G/Y296D/A327G/A330R/P396M	8.9E−09	7.9E−08	1.3E−06	3.0E−09	2.3E−05	26.6	13.7	1040.4
IL6R-BP537/IL6R-L	G237D/P238D/V264I/S267G/H268E/P271G/A330R	2.9E−08	2.7E−07	3.1E−06	6.9E−09	3.6E−05	39.1	4.0	445.9
IL6R-BP538/IL6R-L	G237D/P238D/V264I/H268E/P271G/A330R	5.5E−08	2.0E−07	3.0E−06	5.3E−09	3.3E−05	38.6	5.3	585.2
IL6R-BP539/IL6R-L	G237D/P238D/V264I/S267G/H268E/P271G/E272P/Y296D/A330R	6.4E−08	3.3E−07	5.6E−06	8.4E−09	3.4E−05	39.0	3.3	367.9
IL6R-BP540/IL6R-L	G237D/P238D/V264I/H268E/P271G/E272P/Y296D/A330R	9.6E−08	2.1E−07	4.6E−06	5.7E−09	3.9E−05	36.6	5.1	539.3
IL6R-BP549/IL6R-L	G237D/P238D/S267G/H268E/P271G/A330R	1.8E−08	5.7E−07	1.1E−05	1.6E−08	2.4E−05	35.9	1.9	195.6
IL6R-BP550/IL6R-L	G237D/P238D/V264I/S267G/H268E/P271G/E272D/Y296D/A330R	2.5E−08	3.4E−07	5.0E−06	7.6E−09	4.8E−05	44.2	3.2	405.0
IL6R-BP551/IL6R-L	G237D/P238D/V264I/H268E/P271G/E272D/Y296D/A330R	3.2E−08	2.5E−07	2.8E−06	6.4E−09	4.8E−05	38.1	4.4	480.6
IL6R-BP552/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/E272D/Y296D/A330R	3.2E−09	9.7E−08	1.9E−06	2.6E−09	3.0E−05	37.3	11.2	1193.1
IL6R-BP553/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/E272D/A330R	3.4E−09	8.6E−08	1.4E−06	3.1E−09	2.0E−05	27.8	12.6	1000.0
IL6R-BP554/IL6R-L	G237D/P238D/V264I/S267A/H268E/P271G/E272D/A330R	8.0E−09	1.5E−07	2.3E−06	4.4E−09	2.4E−05	32.7	7.4	695.9
IL6R-BP555/IL6R-L	G237D/P238D/V264I/S267A/H268E/P271G/E272D/Y296D/A330R	9.4E−09	1.6E−07	3.2E−06	4.1E−09	3.0E−05	39.7	6.6	751.8
IL6R-BP556/IL6R-L	G237D/P238D/V264I/S267G/H268E/P271G/Y296D/A330R	4.3E−08	3.0E−07	5.8E−06	8.4E−09	6.0E−05	35.4	3.6	368.7
IL6R-BP557/IL6R-L	G237D/P238D/S267G/H268D/P271G/Y296D/A330R	1.3E−08	8.5E−07	1.5E−05	2.0E−08	2.9E−05	42.0	1.3	153.7
IL6R-BP558/IL6R-L	G237D/P238D/V264I/S267G/H268E/P271G/E272D/A330R	1.3E−08	3.3E−07	4.9E−06	9.0E−09	3.6E−05	36.4	3.3	342.6
IL6R-BP559/IL6R-L	P238D/V264I/S267A/H268E/P271G/E272D/Y296D	1.1E−09	1.6E−06	2.0E−05	2.8E−08	4.4E−05	58.4	0.7	110.0
IL6R-BP560/IL6R-L	P238D/S267G/H268E/P271G/Y296D/A330R	5.6E−09	4.2E−06	3.1E−05	1.8E−07	4.1E−05	22.8	0.3	16.8
IL6R-BP561/IL6R-L	E233D/G237D/P238D/H268D/P271G/E272D/Y296D/A330R	9.4E−09	5.1E−07	5.3E−06	1.8E−08	3.7E−05	28.0	2.1	169.8
IL6R-BP562/IL6R-L	G237D/P238D/H268D/P271G/E272D/Y296D/A330R	2.5E−08	6.8E−07	1.1E−05	2.4E−08	5.3E−05	29.0	1.6	130.9
IL6R-BP563/IL6R-L	E233D/G237D/P238D/H268E/P271G/E272D/Y296D/A330R	1.2E−08	4.6E−07	8.3E−06	1.6E−08	3.8E−05	29.1	2.4	195.6
IL6R-BP564/IL6R-L	G237D/P238D/H268E/P271G/E272D/Y296D/A330R	3.1E−08	5.8E−07	1.0E−05	2.2E−08	4.9E−05	26.2	1.9	140.5
IL6R-BP565/IL6R-L	E233D/G237D/P238D/S267A/H268E/P271G/Y296D/A330R	2.4E−09	2.3E−07	4.7E−06	5.5E−09	2.1E−05	41.5	4.7	562.8
IL6R-BP567/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/Y296D	2.1E−10	8.9E−07	1.7E−05	1.4E−08	3.9E−05	64.4	1.2	223.9
IL6R-BP568/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G	1.9E−10	6.8E−07	1.1E−05	1.5E−08	2.5E−05	46.1	1.6	210.2

[0478] All of the variants in Table 11 had enhanced FcgRIIb binding in comparison to that of IL6R-B3/IL6R-L, and the degree of enhancement of FcgRIIb binding compared to that of IL6R-B3/IL6R-L was 2.6 times to 3090 times. Furthermore, while KD(IIaR)/KD(IIb) for IL6R-B3/IL6R-L was 0.3, those of the variants shown in Table 11 ranged from 8.7 to 64.4, and the selectivities (KD(IIaR)/KD(IIb)) of all the variants for FcgRIIb were improved in comparison to those of IL6R-B3/IL6R-L.

[0479] Introducing alterations that lower binding to all FcgRs into these variants with selectively enhanced FcgRIIb binding was predicted to yield variants whose FcgRIIb-binding is maintained at a level similar to that of IgG1 while binding to other activating FcgRs is selectively lowered in comparison to those of IgG1. Then, it was verified whether variants can be obtained as predicted above, by actually using two types of alterations introduced into IL6R-BP568/IL6R-L and IL6R-BP489/IL6R-L that selectively enhance FcgRIIb binding. Specifically, variants introduced with the above-mentioned two alterations which selectively enhance FcgRIIb binding were used as templates to confirm that it is possible to obtain variants with FcgRIIb binding maintained at a level similar to that of IgG1 but whose binding to other activating FcgRs is selectively lowered in comparison to those of IgG1. More specifically, by introducing into IL6R-G1d alterations that enhance FcgRIIb binding as used in IL6R-BP568/IL6R-L and IL6R-BP489/IL6R-L, two types of variants with selectively enhanced FcgRIIb binding, IL6R-P577 and IL6R-P587, were produced. IL6R-L (SEQ ID NO: 6) was utilized as a common antibody L chain, and together with the respective H chain, the antibodies were expressed and purified according to the method of Reference Example 1. KD values of these two variants for each FcgR are shown in Table 12.

TABLE 12
							KD of	KD of
							G1d for	G1d for
							FcgRIIaR/	FcgRIIb/
							KD of	KD of
		KD	KD	KD	KD	KD	Each Var-	Each Var-
		(M) for	(M) for	(M) for	(M) for	(M) for	iant for	iant for
Variant Name	Introduced Alterations	FcgRIa	FcgRIIaR	FcgRIIaH	FcgRIIb	FcgRIIIaV	FcgRIIaR	FcgRIIb
IL6R-G1d/IL6R-L		1.2E−10	9.7E−07	6.5E−07	3.9E−06	4.2E−07	1.0	1.0
IL6R-P577/IL6R-L	E233D/G237D/P238D/V264I/	1.1E−09	4.4E−08	1.0E−06	1.2E−09	1.2E−05	21.9	3370.7
	S267A/H268E/P271G/
	Y296D/A330R/P396M
IL6R-P587/IL6R-L	E233D/P238D/V264I/S267A/	2.1E−10	7.1E−07	1.2E−05	1.5E−08	2.8E−05	1.4	255.6
	H268E/P271G

[0480] The binding of P577 to FcgRIIaR was 21.9 times that of G1d, and its binding to FcgRIIb was 3370.7 times that of G1d. The binding of P587 to FcgRIIaR was 1.4 times that of G1d, and its binding to FcgRIIb was 255.6 times that of G1d.

[0481] By introducing alterations that lower binding to all FcgRs into the prepared two types of variants with enhanced binding to FcgRIIb, production of variants whose FcgRIIb binding is maintained at a level similar to that of a native IgG1 while binding to other FcgRs, in particular FcgRIIaR, are attenuated as much as possible was examined Alterations that greatly lower FcgRIIaR binding were found through an investigation involving comprehensive introduction of alterations to the FcgR-interacting residues. In addition to the comprehensive introduction of alterations at positions 234, 235, 236, 237, and 239 of IL6R-F652/IL6R-L according to EU numbering as shown in Example 4-1, comprehensive introduction of alterations was carried out on variants with selectively enhanced FcgRIIb binding.

[0482] Alterations that enhance FcgRIIb-binding (E233D/G237D/H268E/P271G) as reported in WO2012/115241 were introduced into IL6R-BF648 (SEQ ID NO: 2) to produce IL6R-BP267 (SEQ ID NO: 5). Variants with substitutions at positions 265, 266, 267, and 269 of IL6R-BP267, according to EU numbering, with 18 types of amino acids excluding the original amino acid and Cys were produced. IL6R-L (SEQ ID NO: 6) was utilized as a common antibody L chain, and together with the respective H chain, the antibodies were expressed and purified according to the method of Reference Example 1. These antibody variants were expressed and purified according to the method of Reference Example 1, and binding to each of the FcγRs (FcγRIa, FcγRIIa H type, FcγRIIa R type, FcgRIIb, FcγRIIIa V type) was comprehensively evaluated by the method of Reference Example 2. The relative FcgRIIaR- and FcgRIIb-binding activities of variants discovered by comprehensively introducing alterations into IL6R-F652/IL6R-L and IL6R-BP267/IL6R-L are shown in Table 13 and Table 14, respectively. These values are obtained by dividing values for the amount of each variant bound to FcgRIIaR or FcgRIIb by values for the amount of IL6R-F652/IL6R-L or IL6R-BP267/IL6R-L bound to respective FcgR, and then multiplying these values by 100.

	TABLE 13
		Alteration	Relative	Relative
		introduced	FcgRIIaR-	FcgRIIb-
		into IL6R-	binding	binding
	Variant Name	F652	Activity	Activity
	IL6R-F652/IL6R-L		100.0	100.0
	IL6R-PD072/IL6R-L	G236Q	20.6	10.4
	IL6R-PD085/IL6R-L	G237K	0.5	11.8
	IL6R-PD090/IL6R-L	G237R	7.9	23.7
	IL6R-PD026/IL6R-L	L234A	67.2	37.2
	IL6R-PD031/IL6R-L	L234H	41.8	27.6
	IL6R-PD033/IL6R-L	L234K	11.2	6.2
	IL6R-PD035/IL6R-L	L234N	44.4	38.3
	IL6R-PD038/IL6R-L	L234R	7.8	5.4
	IL6R-PD044/IL6R-L	L235A	37.7	7.9

	TABLE 14
		Alterations	Relative	Relative
		introduced	FcgRIIaR-	FcgRIIb-
		into IL6R-	binding	binding
	Variant name	BP267	Activity	Activity
	IL6R-BP267/IL6R-L		100.0	100.0
	IL6R-BP318/IL6R-L	D265K	4.3	2.3
	IL6R-BP321/IL6R-L	D265N	16.3	31.7
	IL6R-BP324/IL6R-L	D265R	3.7	3.2
	IL6R-BP325/IL6R-L	D265S	11.6	21.0
	IL6R-BP327/IL6R-L	D265V	ND	ND
	IL6R-BP355/IL6R-L	S267K	11.6	14.4
	IL6R-BP361/IL6R-L	S267R	17.3	27.7
	IL6R-BP365/IL6R-L	S267Y	9.8	16.7

[0483] From the results shown in Tables 13 and 14, the alterations were found to lower FcgRIIaR binding to at least 67% or less in comparison to before introducing the alterations, and they included some that lead to complete loss of FcgRIIaR-binding.

[0484] Next, these alterations were introduced into variants with selectively enhanced FcgRIIb-binding, and then examined. Specifically, 17 alterations shown in Tables 13 and 14 were introduced into IL6R-P577 and IL6R-P587 according to the method of Reference Example 1. It is reported that removing the N-type sugar chain attached to Asn at position 297 of the Fc region, according to EU numbering, remarkably lowers FcgR-binding by antibodies (The Journal of Biological Chemistry, 2000, 276, 6591-6604). Therefore, in this Example, in addition to the 17 alterations described above, N297A was introduced into IL6R-P577 and IL6R-P587 according to the method of Reference Example 1 to remove the N-type sugar chain attached to Asn297. IL6R-L (SEQ ID NO: 6) was utilized as a common antibody L chain, and together with the respective H chain, the antibodies were expressed and purified according to the method of Reference Example 1. Binding of the obtained variants to FcgRIa, FcgRIIaR, FcgRIIaH, FcgRIIb, and FcgRIIIaV was evaluated according to the method of Reference Example 2. Table 15 shows the relative binding activities of each variant to FcgRIIaR and FcgRIIb. These values are obtained by dividing values for the amount of each variant bound to FcgRIIaR or FcgRIIb by values for the amount of IL6R-G1d/IL6R-L bound to FcgRIIaR or FcgRIIb, and then multiplying these values by 100.

TABLE 15
		Relative	Relative
		FcgRIIaR-	FcgRIIb-
		binding	binding
Variant Name	Alterations that are included in the introduction	Activity	Activity
IL6R-G1d/IL6R-L		100.0	100.0
IL6R-F648/IL6R-L	P238D	29.9	138.5
IL6R-P606/IL6R-L	E233D/P238D/S239K/V264I/S267A/H268E/P271G	4.8	7.7
IL6R-P607/IL6R-L	E233D/G237D/P238D/S239K/V264L/S267A/H268E/P271G/Y296D/A330R/P396M	5.1	21.8
IL6R-P609/IL6R-L	E233D/L234A/P238D/V264I/S267A/H268E/P271G	82.6	178.4
IL6R-P611/IL6R-L	E233D/L234A/G237D/P238D/V264L/S267A/H258E/P271G/Y296D/A330R/P396M	107.4	183.3
IL6R-P613/IL6R-L	E233D/L235A/P238D/V264I/S267A/H268E/P271G	61.2	120.3
IL6R-P614/IL6R-L	E233D/L235A/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	69.5	176.0
IL6R-P616/IL6R-L	E233D/L234A/L235A/P238D/V264I/S267A/H268E/P271G	41.8	89.2
IL6R-P617/IL6R-L	E233D/L234A/L235A/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	49.2	157.6
IL6R-P623/IL6R-L	E233D/L234H/P238D/V264I/S267A/H268E/P271G	44.7	151.8
IL6R-P624/IL6R-L	E233D/L234N/P238D/V264I/S267A/H268E/P271G	67.1	172.9
IL6R-P625/IL6R-L	E233D/L234K/P238D/V264I/S267A/H268E/P271G	25.8	141.1
IL6R-P626/IL6R-L	E233D/L234R/P238D/V264I/S267A/H268E/P271G	24.4	129.2
IL6R-P627/IL6R-L	E233D/G238Q/P238D/V264I/S267A/H268E/P271G	48.5	138.5
IL6R-P628/IL6R-L	E233D/G237R/P238D/V264I/S267A/H268E/P271G	48.1	176.5
IL6R-P629/IL6R-L	E233D/G237K/P238D/V264I/S267A/H268E/P271G	29.9	163.9
IL6R-P630/IL6R-L	E233D/P238D/V264I/D265K/S267A/H268E/P271G	4.1	7.1
IL6R-P631/IL6R-L	E233D/P238D/V264I/D265N/S267A/H268E/P271G	26.7	109.8
IL6R-P632/IL6R-L	E233D/P238D/V264I/D265R/S267A/H268E/P271G	4.1	8.0
IL6R-P633/IL6R-L	E233D/P238D/V264I/D265S/S267A/H268E/P271G	15.3	55.4
IL6R-P634/IL6R-L	E233D/P238D/V264I/D265V/S267A/H268E/P271G	2.8	6.5
IL6R-P635/IL6R-L	E233D/P238D/V264I/S267K/H268E/P271G	6.4	71.9
IL6R-P636/IL6R-L	E233D/P238D/V264I/S267R/H268E/P271G	13.8	119.8
IL6R-P637/IL6R-L	E233D/P238D/V264I/S267Y/H268E/P271G	9.1	55.5
IL6R-P638/IL6R-L	E233D/L234H/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	78.3	170.4
IL6R-P639/IL6R-L	E233D/L234N/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	108.7	184.2
IL6R-P640/IL6R-L	E233D/L234K/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	75.1	178.3
IL6R-P641/IL6R-L	E233D/L234R/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	61.5	170.1
IL6R-P642/IL6R-L	E233D/G236Q/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	103.2	175.0
IL6R-P643/IL6R-L	E233D/G237R/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	58.7	177.7
IL6R-P644/IL6R-L	E233D/G237K/P238D/V264I/S267A/H268E/P271G/Y296D/A330R/P396M	34.0	170.8
IL6R-P645/IL6R-L	E233D/G237D/P238D/V264I/D265K/S267A/H268E/P271G/Y296D/A330R/P396M	5.2	13.7
IL6R-P646/IL6R-L	E233D/G237D/P238D/V264I/D265N/S267A/H268E/P271G/Y296D/A330R/P396M	72.5	175.3
IL6R-P647/IL6R-L	E233D/G237D/P238D/V264I/D265R/S267A/H268E/P271G/Y296D/A330R/P396M	5.6	15.2
IL6R-P648/IL6R-L	E233D/G237D/P238D/V264I/D265S/S267A/H268E/P271G/Y296D/A330R/P396M	40.3	152.3
IL6R-P649/IL6R-L	E233D/G237D/P238D/V264I/D265V/S267A/H268E/P271G/Y296D/A330R/P396M	2.9	6.9
IL6R-P650/IL6R-L	E233D/G237D/P238D/V264I/S267K/H268E/P271G/Y296D/A330R/P396M	41.6	154.9
IL6R-P651/IL6R-L	E233D/G237D/P238D/V264I/S267R/H268E/P271G/Y296D/A330R/P396M	75.7	168.5
IL6R-P652/IL6R-L	E233D/G237D/P238D/V264I/S267Y/H268E/P271G/Y296D/A330R/P396M	27.5	148.8
IL6R-P664/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/N297A	10.8	33.9
IL6R-P665/IL6R-L	E233D/G237D/P238D/V264I/S267A/H268E/P271G/Y296D/N297A/A330R/P396M	15.0	121.4
IL6R-P696/IL6R-L	E233D/G237R/P238D/V264I/S267R/H268E/P271G	6.1	73.7
IL6R-P697/IL6R-L	E233D/G237K/P238D/V264I/S267R/H268E/P271G	3.6	28.7
IL6R-P700/IL6R-L	E233D/L234A/P238D/V264I/S267R/H268E/P271G	5.8	38.1
IL6R-P701/IL6R-L	E233D/L234N/P238D/V264I/S267R/H268E/P271G	7.0	47.8

[0485] As shown in Table 15, variants P606 and P607 produced by introducing S239K, P630 and P645 produced by introducing D265K, P632 and P647 produced by introducing D265R, and P634 and P649 produced by introducing D265V showed hardly any binding to FcgRIIaR and FcgRIIb. This means that these alterations will remarkably reduce the FcgRIIb-binding activity even if they are introduced into variants with enhanced FcgRIIb binding. On the other hand, P636 produced by introducing S267R into P587, and P665 produced by introducing N297A into P577 showed almost the same FcgRIIb-binding as that of G1d and greatly reduced FcgRIIaR-binding.

[0486] From among the variants shown in Table 15, variants with FcgRIIb binding maintained at 80% or more of the binding activity of G1d, and FcgRIIaR binding suppressed to 30% or less of the binding activity of G1d, and their KD values for each FcgR are shown in Table 16. The relative binding activity in the table is a value obtained by dividing the KD value of IL6R-G 1 d/IL6R-L by the KD value of each variant, and represents the relative binding activity of each variant when the KD value of IL6R-G1d/IL6R-L for each FcgR is defined as 1. Among the KD values shown in the table, the values in the solid gray boxes are values calculated by utilizing Equation 2 of Reference Example 2, as the binding of FcgR to each variant is very weak and cannot be accurately analyzed by kinetic analysis.

KD=C·R_max/(R_eq−RI)−C  [Equation 2]

TABLE 16
		KD	KD	KD	KD
		(M) for	(M) for	(M) for	(M) for
Variant name	Included Alterations	FcgRIa	FcgRIIaR	FcgRIIaH	FcgRIIb
IL6R-G1d/IL6R-L		3.3E−10	1.2E−06	9.6E−07	5.1E−06
IL6R-F648/IL6R-L	P238D	8.0E−09	1.9E−05	7.9E−05	1.9E−06
IL6R-P625/IL6R-L	E233D/L234K/P238D/V264I/S267A/H268E/P271G	5.0E−07	2.4E−05	1.8E−04	1.7E−06
IL6R-P626/IL6R-L	E233D/L234R/P238D/V264I/S267A/H268E/P271G	7.4E−07	3.1E−05	2.0E−04	2.7E−06
IL6R-P629/IL6R-L	E233D/G237K/P238D/V264I/S267A/H268E/P271G	3.3E−09	2.7E−05	1.0E−04	6.5E−07
IL6R-P631/IL6R-L	E233D/P238D/V264I/D265N/S267A/H268E/P271G	1.1E−08	2.6E−05	9.6E−05	4.4E−06
IL6R-P636/IL6R-L	E233D/P238D/V264I/S267R/H268E/P271G	4.6E−07	5.4E−05	1.7E−04	2.9E−06
IL6R-P652/IL6R-L	E233D/G237D/P238D/V264I/S267Y/H268E/P271G/	7.2E−07	2.4E−05	1.7E−04	1.2E−06
	Y296D/A330R/P396M
IL6R-P665/IL6R-L	E233D/G237D/P238D/V2641/S267A/H268E/P271G/	1.9E−06	4.9E−05	4.5E−04	3.2E−06
	Y296D/N297A/A330R/P396M
			Relative	Relative	Relative	Relative	Relative
		KD	FcgRIa-	FcgRIIaR-	FcgRIIaH-	FcgRIIb-	FcgRIIIaV-
		(M) for	binding	binding	binding	binding	binding
	Variant name	FcgRIIIaV	Activity	Activity	Activity	Activity	Activity
	IL6R-G1d/IL6R-L	3.4E−07	1.0000	1.000	1.000	1.000	1.000
	IL6R-F648/IL6R-L	6.9E−05	0.0413	0.065	0.012	2.749	0.005
	IL6R-P625/IL6R-L	6.6E−05	0.0007	0.051	0.005	3.060	0.005
	IL6R-P626/IL6R-L	7.5E−05	0.0004	0.041	0.005	1.897	0.005
	IL6R-P629/IL6R-L	4.7E−05	0.1000	0.047	0.010	7.969	0.007
	IL6R-P631/IL6R-L	4.7E−05	0.0300	0.048	0.010	1.158	0.007
	IL6R-P636/IL6R-L	4.5E−05	0.0007	0.023	0.006	1.772	0.008
	IL6R-P652/IL6R-L	7.2E−05	0.0005	0.051	0.006	4.179	0.005
	IL6R-P665/IL6R-L	6.7E−05	0.0002	0.025	0.002	1.601	0.005

[0487] Among these variants, the one showing the most reduced FcgRIIaR binding was P636 produced by introducing S267R into P587. The KD of P636 for FcgRIIaR was 0.023 times that of G1d, but the KD for FcgRIIb was maintained at 1.8 times that of G1d. Furthermore, FcgRIa binding was suppressed to 0.0007 times that of G1d, FcgRIIaH-binding was suppressed to 0.006 times, and FcgRIIIaV binding was suppressed to 0.008 times. The above-mentioned results showed that it is possible to obtain variants whose FcgRIIb binding is maintained at a level similar to that of IgG1 while binding to other FcgRs is lowered in comparison to those of IgG1 by introducing alterations that lower binding to all FcgRs into a variant with selectively enhanced FcgRIIb binding.

(4-3) Examination of a Combination of Alterations that have an Effect of Lowering FcgRIIaR Binding

[0488] Production of better variants was examined by combining alterations found in 4-1 and 4-2 that maintain FcgRIIb binding at a level similar to that of G1d while lowering FcgRIIaR binding.

[0489] Results of examining the combinations are shown in Table 17. The relative binding activities shown in the table are values obtained by dividing the KD value of IL6R-G1d/IL6R-L by the KD value of each variant, and represent the relative binding activities for each variant when the KD value of IL6R-G1d/IL6R-L for each FcgR is defined as 1. Among the KD values shown in the table, the values in the solid gray boxes are values calculated by utilizing Equation 2 of Reference Example 2, as the binding of FcgR to each variant is very weak and cannot be accurately analyzed by kinetic analysis.

KD=C·R_max/(R_eq−RI)−C  [Equation 2]

TABLE 17
		KD	KD	KD	KD
		(M) for	(M) for	(M) for	(M) for
Variant name	Included alterations	FcgRIa	FcgRIIaR	FcgRIIaH	FcgRIIb
IL6R-G1d/IL6R-L		3.3E−10	1.2E−06	9.6E−07	5.1E−06
IL6R-F648/IL6R-L	P238D	8.0E−09	1.9E−05	7.9E−05	1.9E−06
IL6R-P694/IL6R-L	E233D/P238D/V264I/S267R/H268E/P271G/Y296E	4.1E−07	0.1E−05	2.0E−04	2.8E−06
IL6R-P699/IL6R-L	E233D/P238D/V264I/S267R/H268P/P271G	6.9E−07	5.7E−05	2.5E−04	4.1E−06
IL6R-P702/IL6R-L	E233D/P238D/F241M/V264I/S267R/H268E/P271G	5.7E−07	5.4E−05	1.7E−04	4.0E−06
IL6R-P712/IL6R-L	E233D/P238D/V264I/S267R/H268P/P271G/Y296E	6.8E−07	7.1E−05	3.0E−04	3.4E−06
IL6R-P730/IL6R-L	E233D/G237Q/P238D/V264I/S267R/H268P/P271G/Y296E	1.5E−06	9.5E−05	3.1E−04	1.0E−05
			Relative	Relative	Relative	Relative	Relative
		KD	FcgRIa-	FcgRIIaR-	FcgRIIaH-	FcgRIIb-	FcgRIIIaV-
		(M) for	binding	binding	binding	binding	binding
	Variant name	FcgRIIIaV	Activity	Activity	Activity	Activity	Activity
	IL6R-G1d/IL6R-L	3.4E−07	1.000	1.000	1.000	1.000	1.000
	IL6R-F648/IL6R-L	6.9E−05	0.0413	0.065	0.012	2.749	0.005
	IL6R-P694/IL6R-L	4.7E−05	0.0008	0.020	0.005	1.829	0.007
	IL6R-P699/IL6R-L	6.5E−05	0.0005	0.022	0.004	1.263	0.005
	IL6R-P702/IL6R-L	4.4E−05	0.0006	0.023	0.006	1.275	0.008
	IL6R-P712/IL6R-L	8.2E−05	0.0005	0.017	0.003	1.516	0.004
	IL6R-P730/IL6R-L	7.3E−05	0.0002	0.013	0.003	0.504	0.005

[0490] Among the variants shown in Table 17, the one showing the most reduced FcgRIIaR binding was P712 produced by introducing a combination of S267R, H268P, and Y296E into P587, which is a variant with selectively enhanced FcgRIIb binding; and its FcgRIIb binding was maintained at 1.5 times that of G1d while its FcgRIIaR-binding was lowered to 0.017 times that of G1d. Furthermore, its FcgRIa binding was suppressed to 0.0005 times that of G1d, FcgRIIaH binding was suppressed to 0.003 times, and FcgRIIIaV binding was suppressed to 0.004 times.

[0491] Similarly to ADCC, complement-dependent cytotoxicity (CDC) is an effector function that initiates immune response. The variants produced so far all have greatly reduced binding activities for activating receptors except for FcgRIIb, and their ADCC activities are considered to be greatly attenuated. However, since the binding site between an antibody and a complement is different from the binding site between an antibody and an FcgR, it is possible that the complement-binding activity is maintained. Therefore, the complement-binding activity of each variant was evaluated, and variants with attenuated complement-binding were produced by combining alterations that lower complement-binding.

[0492] The interaction between an antibody and human C1q was analyzed using Biacore T200 (GE Healthcare). HBS-EP+ (GE Healthcare) was used as the running buffer, and the measurement temperature was set at 25° C. Chips produced by immobilizing Protein L (ACTIGEN or BioVision) onto Series S sensor Chip CM4 (GE Healthcare) by amine coupling were used.

[0493] The antibodies of interest were captured onto these sensor chips, and allowed to interact with human complement C1q (PROSPEC or Calbiochem) diluted in the running buffer. The amount of antibody binding was determined, and comparisons were made among the antibodies. However, since the amount of C1q bound depends on the amount of captured antibodies, corrected values obtained by dividing the amount of C1q bound by the amount of each antibody captured were compared. Furthermore, by reaction with 10 mM glycine-HCl, pH 1.5, the antibodies captured onto the sensor chips were washed, and the sensor chips were recycled and reused.

[0494] For the alteration to lower C1q binding, K322A described in a prior document (J. Immunol, 2003, 164, 4178-4184) was used. Furthermore, since substitution of oppositely charged Glu for Lys at position 322 according to EU numbering was also expected to lower C1q binding, K322E was also examined. Furthermore, the remarkably low CDC activity of IgG4 in comparison to IgG1 has been reported to be caused by difference in the C-terminal sequences of the CH2 domains (J. Exp. Med., 1991, 173, 1025-1028). Therefore, lowering C1q binding by substituting Gly for Ala at position 327, Ser for Ala at position 330, and Ser for Pro at position 331 of IgG1, according to EU numbering, to produce an IgG4-type sequence was also examined.

[0495] Human C1q binding was evaluated for variants with enhanced FcgRIIb binding and variants with maintained FcgRIIb binding as well as lowered binding to other FcgRs. Variants were produced by combining the above variants with alterations that lower C1q binding, and the variants were evaluated. Furthermore, binding to each FcgR was evaluated for all variants produced according to the method of Reference Example 2. Human IgG4 was used as a negative control for evaluation of C1q binding. IL6R-G4d (SEQ ID NO: 52) comprising G4d prepared by substituting Pro for Ser at position 228 according to EU numbering and removing Gly and Lys from the C terminus of human IgG4, was produced. IL6R-L (SEQ ID NO: 6) was utilized as a common antibody L chain.

[0496] Table 18 shows the results of evaluating the binding of the produced variants to human C1q. “Amount of C1q binding when that for G1d is defined as 100” in the table is a value obtained by dividing the amount of C1q bound to each variant by the amount of each variant captured, then dividing it by the value obtained by dividing the amount of C1q bound to IL6R-G1d/IL6R-L by the amount of IL6R-G1d/IL6R-L captured, and then multiplying that value by 100. More specifically, it is a value that shows the level of C1q binding in comparison to that of IL6R-G1d/IL6R-L.

TABLE 18
		Amount of
		C1q-binding
		when that for
		G1d is Defined
Variant name	Included alterations	as 100
IL6R-G1d/IL6R-L		100.0
IL6R-G4d/IL6R-L		15.5
IL6R-G1dK322A/IL6R-L	K3322A	20.5
IL6R-G1dK322E/IL6R-L	K322E	2.3
IL6R-G1dGSS/IL6R-L	A327G/A330S/P331S	15.2
IL6R-P648/IL6R-L	P238D	21.5
IL6R-P741/IL6R-L	P238D/K322A	1.4
IL6R-P742/IL6R-L	P238D/K322E	14.1
IL6R-P743/IL6R-L	P238D/A327G/A330S/P331S	20.9
IL6R-P600/IL6R-L	P238D/S298A	2.4
IL6R-P744/IL6R-L	P238D/S298A/K322A	13.6
IL6R-P745/IL6R-L	P238D/S298A/K322E	20.9
IL6R-P691/IL6R-L	L235F/G237Q/P238D/F241M/Y296E	21.0
IL6R-P746/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/K322A	1.4
IL6R-P747/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/K322E	13.7
IL6R-P727/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/A330H	35.5
IL6R-P748/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322A/A330H	1.4
IL6R-P749/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322E/A330H	13.8
IL6R-P729/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E	22.8
IL6R-P750/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322A	1.2
IL6R-P751/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322E	13.9
IL6R-P733/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/S324H	23.0
IL6R-P752/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322A/S324H	1.1
IL6R-P753/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322E/S324H	14.0
IL6R-P737/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/S324H/A330H	45.7
IL6R-P754/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322A/S324H/A330H	1.8
IL6R-P755/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322E/S324H/A330H	14.4
IL6R-P587/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G	131.7
IL6R-P588/IL6R-L	P238D/V264I/S267A/H268E/P271G	108.1
IL6R-P756/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/A327G/A330S/P331S	15.0
IL6R-P757/IL6R-L	P238D/V264I/S267A/H268E/P271G/A327G/A330S/P331S	22.6
IL6R-P758/IL6R-L	G237D/P238D/H268D/P271G/A327G/A330S/P331S	2.0
IL6R-P759/IL6R-L	E233D/G237D/P238D/H268D/P271G/A327G/A330S/P331S	14.5
IL6R-P760/IL6R-L	G237D/P238D/H268E/P271G/A327G/A330S/P331S	21.7
IL6R-P761/IL6R-L	E233D/G237D/P238D/H268E/P271G/A327G/A330S/P331S	2.1
IL6R-P762/IL6R-L	P238D/P271G/A327G/A330S/P331S	14.6
IL6R-P766/IL6R-L	P238D/S267A/H268E/P271G/A327G/A330S/P331S	21.8
IL6R-P767/IL6R-L	P238D/V264I/H268E/P271G/A327G/A330S/P331S	1.7
IL6R-P768/IL6R-L	P238D/V264I/S267A/P271G/A327G/A330S/P331S	14.0
IL6R-P769/IL6R-L	P238D/V264I/P271G	31.3
IL6R-P770/IL6R-L	P238D/V264I/P271G/A327G/A330S/P331S	1.5
IL6R-P771/IL6R-L	P238D/V264I/P271G/K322A	14.3
IL6R-P772/IL6R-L	P238D/V264I/P271G/K322E	21.6
IL6R-P773/IL6R-L	P238D/S267A/H268E/P271G/K322A	1.7
IL6R-P774/IL6R-L	P238D/S267A/H268E/P271G/K322E	15.2
IL6R-P112/IL6R-L	P238D/P271G	24.2
IL6R-P555/IL6R-L	G237D/P238D/H268D/P271G	29.1
IL6R-P556/IL6R-L	E233D/G237D/P238D/H268D/P271G	33.5
IL6R-P559/IL6R-L	G237D/P238D/H268E/P271G	34.1
IL6R-P562/IL6R-L	E233D/G237D/P238D/H268E/P271G	29.4
IL6R-P763/IL6R-L	P238D/S267A/H268E/P271G	41.7
IL6R-P764/IL6R-L	P238D/V264I/H268E/P271G	23.1
IL6R-P765/IL6R-L	P238D/V264I/S267A/P271G	46.6
IL6R-P775/IL6R-L	P238D/P271G/K322A	21.8
IL6R-P776/IL6R-L	P238D/P271G/K322E	0.7
IL6R-P777/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/K322A	12.4
IL6R-P778/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/K322E	23.9
IL6R-P779/IL6R-L	P238D/V264I/S267A/H268E/P271G/K322A	0.8
IL6R-P780/IL6R-L	P238D/V264I/S267A/H268E/P271G/K322E	12.9
IL6R-P781/IL6R-L	P238D/S298A/A327G/A330S/P331S	24.0
IL6R-P782/IL6R-L	E233D/P238D/A327G/A330S/P331S	1.3
IL6R-P783/IL6R-L	G237D/P238D/A327G/A330S/P331S	13.3
IL6R-P784/IL6R-L	E233D/G237D/P238D/A327G/A330S/P331S	24.2
IL6R-P785/IL6R-L	E233D/P238D/S298A/A327G/A330S/P331S	1.3
IL6R-P786/IL6R-L	G237D/P238D/S298A/A327G/A330S/P331S	12.9
IL6R-P787/IL6R-L	E233D/G237D/P238D/S298A/A327G/A330S/P331S	24.0

[0497] When the value for G1d comprising the native sequence was defined as 100, the value for the negative control, G4d, was 15.5. C1q binding of G1dK322A, G1dK322E, and G1dGSS which have been produced by introducing into G1d alterations that lower C1q binding had values of 20.5, 2.3, and 15.2, respectively, which were values equivalent to or lower than that of G4d. This showed that C1q binding was greatly lowered compared to before introducing the alterations. Furthermore, F648 produced by introducing the P238D alteration which selectively enhances FcgRIIB binding was found to have C1q binding nearly equal to that of G4d even without the use of alterations that lower C1q binding. The C1q-binding ability of P741, P742, and P743 produced by further introducing alterations that lower C1q binding into these variants all had values equivalent to or less than that of G4d.

[0498] Among P600, P691, P727, P729, P733, and P737 which are variants with their FcgRIIb-binding maintained at the same level as that of the native type and attenuated ability to bind to other FcgRs, P600, P691, P729, and P733 all had a C1q-binding activity equivalent to that of G4d. On the other hand, while P727 and P737 showed largely attenuated binding abilities compared to that of G1d, they were two-fold or higher in comparison to that of G4d. The A330H alteration is commonly introduced into both variants, and this is thought to enhance C1q binding. By introducing into all variants K322A or K322E which are alterations that lower C1q binding, the C1q-binding activity was suppressed to a level lower than that of G4d.

[0499] Among P587, P588, P769, P112, P555, P556, P559, P562, P763, P764, and P765 which are variants with enhanced FcgRIIb binding, P587 and P588 have been found to have C1q binding equal to or greater than that of G1d. Furthermore, in comparison to G1d, the binding abilities of P769, P556, P559, P562, P763, and P765 were greatly attenuated; however, they were nearly twice that of G4d. On the other hand, the C1q-binding activities of P112 and P764 were nearly equal to that of G4d. Furthermore, introducing alterations that lower C1q binding suppressed the C1q-binding activities in all variants to levels equivalent to or lower than that of G4d.

[0500] Table 19 shows the relative binding activities of each variant for FcgRIIaR and FcgRIIb. These values are obtained by dividing values for the amount of each variant bound to FcgRIIaR or FcgRIIb by values for the amount of IL6R-G1d/IL6R-L bound to FcgRIIaR or FcgRIIb, and then multiplying the values by 100.

TABLE 19
		Relative	Relative
		FcgRIIaR-	FcgRIIb-
		binding	binding
Variant name	Alterations Introduced into G1d	Activity	Activity
IL6R-G1d/IL6R-L		100.0	100.0
IL6R-G1dK322A/IL6R-L	K322A	105.2	108.6
IL6R-G1dK322E/IL6R-L	K322E	111.7	129.1
IL6R-G1dGSS/IL6R-L	A327G/A330S/P331S	98.5	115.2
IL6R-F648/IL6R-L	P238D	30.7	185.3
IL6R-P741/IL6R-L	P238D/K322A	20.5	162.8
IL6R-P742/IL6R-L	P238D/K322E	26.6	171.0
IL6R-P743/IL6R-L	P238D/A327G/A330S/P331S	13.5	69.4
IL6R-P600/IL6R-L	P238D/S298A	15.6	101.6
IL6R-P744/IL6R-L	P238D/S298A/K322A	11.1	81.4
IL6R-P745/IL6R-L	P238D/S298A/K322E	13.7	95.4
IL6R-P691/IL6R-L	L235F/G237Q/P238D/F241M/Y296E	10.8	119.2
IL6R-P746/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/K322A	7.0	57.5
IL6R-P747/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/K322E	10.0	98.1
IL6R-P727/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/A330H	9.7	93.0
IL6R-P748/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322A/A330H	5.1	42.7
IL6R-P749/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322E/A330H	6.8	73.9
IL6R-P729/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E	9.0	100.2
IL6R-P750/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322A	6.1	48.1
IL6R-P751/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322E	7.4	72.5
IL6R-P733/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/S324H	8.1	84.8
IL6R-P752/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322A/S324H	7.2	62.9
IL6R-P753/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322E/S324H	10.4	103.3
IL6R-P737/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/S324H/A330H	8.0	78.7
IL6R-P754/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322A/S324H/A330H	5.6	85.8
IL6R-P755/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322E/S324H/A330H	9.8	130.2
IL6R-P587/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G	106.3	349.5
IL6R-P588/IL6R-L	P238D/V264I/S267A/H268E/P271G	100.5	346.0
IL6R-P756/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/A327G/A330S/P331S	92.4	323.4
IL6R-P757/IL6R-L	P238D/V264I/S267A/H268E/P271G/A327G/A330S/P331S	89.2	326.8
IL6R-P758/IL6R-L	G237D/P238D/H268D/P271G/A327G/A330S/P331S	90.6	285.1
IL6R-P759/IL6R-L	E233D/G237D/P238D/H268D/P271G/A327G/A330S/P331S	96.5	304.7
IL6R-P760/IL6R-L	G237D/P238D/H268E/P271G/A327G/A330S/P331S	97.1	304.1
IL6R-P761/IL6R-L	E233D/G237D/P238D/H268E/P271G/A327G/A330S/P331S	103.3	319.7
IL6R-P762/IL6R-L	P238D/P271G/A327G/A330S/P331S	75.8	252.2
IL6R-P766/IL6R-L	P238D/S267A/H268E/P271G/A327G/A330S/P331S	88.1	285.8
IL6R-P767/IL6R-L	P238D/V264I/H268E/P271G/A327G/A330S/P331S	84.2	317.9
IL6R-P768/IL6R-L	P238D/V264I/S267A/P271G/A327G/A330S/P331S	80.9	305.2
IL6R-P769/IL6R-L	P238D/V264I/P271G	86.7	196.2
IL6R-P770/IL6R-L	P238D/V264I/P271G/A327G/A330S/P331S	78.2	188.5
IL6R-P771/IL6R-L	P238D/V264I/P271G/K322A	75.8	195.3
IL6R-P772/IL6R-L	P238D/V264I/P271G/K322E	84.3	195.5
IL6R-P773/IL6R-L	P238D/S267A/H268E/P271G/K322A	87.3	192.3
IL6R-P774/IL6R-L	P238D/S267A/H268E/P271G/K322E	98.8	199.6
IL6R-P112/IL6R-L	P238D/P271G	75.7	287.2
IL6R-P555/IL6R-L	G237D/P238D/H268D/P271G	83.8	305.0
IL6R-P556/IL6R-L	E233D/G237D/P238D/H268D/P271G	81.7	294.0
IL6R-P559/IL6R-L	G237D/P238D/H268E/P271G	89.4	318.7
IL6R-P562/IL6R-L	E233D/G237D/P238D/H268E/P271G	89.7	311.0
IL6R-P763/IL6R-L	P238D/S267A/H268E/P271G	100.7	331.9
IL6R-P764/IL6R-L	P238D/V264I/H268E/P271G	89.5	328.6
IL6R-P765/IL6R-L	P238D/V264I/S267A/P271G	93.2	339.8
IL6R-P775/IL6R-L	P238D/P271G/K322A	64.2	180.5
IL6R-P776/IL6R-L	P238D/P271G/K322E	77.4	189.7
IL6R-P777/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/K322A	97.5	198.1
IL6R-P778/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/K322E	105.1	198.4
IL6R-P779/IL6R-L	P238D/V264I/S267A/H268E/P271G/K322A	96.0	200.8
IL6R-P780/IL6R-L	P238D/V264I/S267A/H268E/P271G/K322E	101.4	199.9
IL6R-P781/IL6R-L	P238D/S298A/A327G/A330S/P331S	7.8	51.1
IL6R-P782/IL6R-L	E233D/P238D/A327G/A330S/P331S	14.2	95.1
IL6R-P783/IL6R-L	G237D/P238D/A327G/A330S/P331S	20.5	97.9
IL6R-P784/IL6R-L	E233D/G237D/P238D/A327G/A330S/P331S	28.6	118.5
IL6R-P785/IL6R-L	E233D/P238D/S298A/A327G/A330S/P331S	9.1	58.4
IL6R-P786/IL6R-L	G237D/P238D/S298A/A327G/A330S/P331S	11.5	62.4
IL6R-P787/IL6R-L	E233D/G237D/P238D/S298A/A327G/A330S/P331S	14.7	84.9

[0501] Variants containing alterations that lower complement-binding shown in Table 19 had a relative FcgRIIaR-binding activity 105% or less when compared to that of G1d, and a relative FcgRIIb-binding activity maintained at 48% or more when compared to that of G1d.

[0502] Binding of these variants to each FcgR is shown in Table 20. The relative binding activity in the table is a value obtained by dividing the KD value of IL6R-G1d/IL6R-L by the KD value of each variant, and represents the relative binding activity of each variant when the KD value of IL6R-G1d/IL6R-L for each FcgR is defined as 1. Among the KD values shown in the table, the values in the solid gray boxes are values calculated by utilizing Equation 2 of Reference Example 2, as the binding of FcgR to each variant is very weak and cannot be accurately analyzed by kinetic analysis.

KD=C·R_max/(R_eq−RI)·C  [Equation 2]

TABLE 20
							Relative	Relative	Relative	Relative	Relative
		KD	KD	KD	KD	KD	FcgRIa-	FcgRIIaR-	FcgRIIaH-	FcgRIIb-	FcgRIIIaV-
	Alterations introduced	(M) for	(M) for	(M) for	(M) for	(M) for	binding	binding	binding	binding	binding
Variant name	into IL6R-G1d	FcgRIa	FcgRIIaR	FcgRIIaH	FcgRIIb	FcgRIIIaV	Activity	Activity	Activity	Activity	Activity
IL6R-G1d/IL6R-L		3.2E−10	1.1E−06	6.2E−07	2.1E−06	3.7E−07	1.000	1.00	1.000	1.0	1.000
IL6R-G1dK322A/IL6R-L	K322A	2.5E−10	9.5E−07	9.0E−07	2.1E−06	5.0E−07	1.280	1.16	0.689	1.0	0.740
IL6R-G1dK322E/IL6R-L	K322E	1.8E−10	8.1E−07	6.8E−07	2.0E−06	4.7E−07	1.778	1.36	0.912	1.1	0.787
IL6R-G1dGSS/IL6R-L	A327G/A330S/P331S	3.3E−10	1.4E−06	1.2E−06	2.4E−06	1.6E−06	0.970	0.79	0.517	0.9	0.231
IL6R-F648/IL6R-L	P238D	6.4E−09	1.6E−05	3.7E−05	1.2E−06	5.8E−05	0.050	0.07	0.017	1.8	0.006
IL6R-P741/IL6R-L	P238D/K322A	1.3E−08	2.0E−05	6.4E−05	1.5E−06	9.4E−05	0.025	0.06	0.010	1.4	0.004
IL6R-P742/IL6R-L	P238D/K322E	8.2E−09	1.6E−05	3.6E−05	1.2E−06	5.3E−05	0.039	0.07	0.017	1.8	0.007
IL6R-P743/IL6R-L	P238D/A327G/A330S/P331S	1.8E−08	2.0E−05	4.6E−05	3.6E−06	5.7E−05	0.018	0.06	0.013	0.6	0.006
IL6R-P600/IL6R-L	P238D/S298A	3.6E−09	3.9E−05	5.2E−05	3.2E−06	5.7E−05	0.089	0.03	0.012	0.7	0.006
IL6R-P744/IL6R-L	P238D/S298A/K322A	1.0E−08	1.8E−05	4.8E−05	3.2E−06	5.4E−05	0.032	0.06	0.013	0.7	0.007
IL6R-P745/IL6R-L	P238D/S298A/K322E	5.0E−09	2.6E−05	4.4E−05	3.1E−06	5.0E−05	0.064	0.04	0.014	0.7	0.007
IL6R-P691/IL6R-L	L235F/G237Q/P238D/F241M/Y296E	1.3E−07	2.8E−05	6.2E−05	2.3E−06	8.8E−05	0.002	0.04	0.010	0.9	0.004
IL6R-P746/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/K322A	7.4E−09	1.8E−05	6.0E−05	3.0E−06	7.0E−05	0.043	0.06	0.010	0.7	0.005
IL6R-P747/IL6R-L	L235F/G237Q/P238D/F241M/Y296E/K322E	9.0E−08	2.7E−05	5.1E−05	2.7E−06	6.2E−05	0.004	0.04	0.012	0.8	0.006
IL6R-P727/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/A330H	7.1E−08	3.4E−05	4.2E−05	2.7E−06	5.5E−05	0.005	0.03	0.015	0.8	0.007
IL6R-P748/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322A/A330H	1.0E−07	3.9E−05	6.0E−05	6.2E−06	7.7E−05	0.003	0.03	0.010	0.3	0.005
IL6R-P749/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322E/A330H	5.2E−08	2.2E−05	7.0E−05	4.0E−06	8.6E−05	0.006	0.05	0.009	0.5	0.004
IL6R-P729/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E	2.2E−07	1.6E−05	4.7E−05	3.0E−06	5.7E−05	0.001	0.07	0.013	0.7	0.006
IL6R-P750/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322A	2.3E−07	4.9E−05	4.8E−05	4.0E−06	5.7E−05	0.001	0.02	0.013	0.5	0.006
IL6R-P751/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322E	2.4E−07	1.9E−05	4.9E−05	3.5E−06	5.6E−05	0.001	0.06	0.013	0.6	0.007
IL6R-P733/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/S324H	2.0E−07	1.3E−05	4.4E−05	4.5E−06	5.5E−05	0.002	0.08	0.014	0.5	0.007
IL6R-P752/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322A/S324H	1.5E−07	4.1E−05	4.8E−05	4.2E−06	5.9E−05	0.002	0.03	0.013	0.5	0.006
IL6R-P753/IL6R-L	L235F/G237Q/P238D/F241M/H268P/Y296E/K322E/S324H	1.1E−07	1.8E−05	4.3E−05	2.8E−06	5.5E−05	0.003	0.06	0.014	0.8	0.007
IL6R-P737/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/S324H/A330H	4.6E−08	3.7E−05	4.0E−05	4.3E−06	5.6E−05	0.007	0.03	0.016	0.5	0.007
IL6R-P754/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322A/S324H/A330H	1.9E−07	1.0E−05	2.2E−04	7.7E−06	1.0E−04	0.002	0.11	0.003	0.3	0.004
IL6R-P755/IL6R-L	G237Q/P238D/F241M/H268P/Y296E/K322E/S324H/A330H	8.5E−08	1.7E−05	1.3E−04	4.1E−06	6.9E−05	0.004	0.06	0.005	0.5	0.005
IL6R-P587/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G	7.1E−10	1.2E−06	1.4E−05	2.8E−08	3.2E−05	0.451	0.92	0.044	75.0	0.012
IL6R-P588/IL6R-L	P238D/V264I/S267A/H268E/P271G	1.4E−09	1.6E−06	1.8E−05	3.8E−08	3.3E−05	0.229	0.69	0.034	55.3	0.011
IL6R-P756/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/A327G/A330S/P331S	1.1E−09	1.7E−06	2.1E−05	5.2E−08	5.3E−05	0.291	0.65	0.030	40.4	0.007
IL6R-P757/IL6R-L	P238D/V264I/S267A/H268E/P271G/A327G/A330S/P331S	1.7E−09	2.3E−06	2.4E−05	1.0E−07	4.7E−05	0.188	0.48	0.026	21.0	0.008
IL6R-P758/IL6R-L	G237D/P238D/H268D/P271G/A327G/A330S/P331S	1.1E−08	1.9E−06	3.0E−05	2.6E−07	6.2E−05	0.029	0.58	0.021	8.1	0.006
IL6R-P759/IL6R-L	E233D/G237D/P238D/H268D/P271G/A327G/A330S/P331S	5.5E−09	1.4E−06	2.6E−05	1.5E−07	7.6E−05	0.058	0.79	0.024	14.0	0.005
IL6R-P760/IL6R-L	G237D/P238D/H268E/P271G/A327G/A330S/P331S	1.5E−08	1.6E−06	2.3E−05	1.9E−07	4.7E−05	0.021	0.69	0.027	11.1	0.008
IL6R-P761/IL6R-L	E233D/G237D/P238D/H268E/P271G/A327G/A330S/P331S	8.0E−09	1.1E−06	1.7E−05	1.2E−07	4.8E−05	0.040	1.00	0.036	17.5	0.008
IL6R-P762/IL6R-L	P238D/P271G/A327G/A330S/P331S	6.1E−09	3.4E−06	3.0E−05	5.5E−07	5.5E−05	0.052	0.32	0.021	3.8	0.007
IL6R-P766/IL6R-L	P238D/S267A/H268E/P271G/A327G/A330S/P331S	9.7E−10	2.2E−06	2.5E−05	2.9E−07	4.4E−05	0.330	0.50	0.025	7.2	0.008
IL6R-P767/IL6R-L	P238D/V264I/H268E/P271G/A327G/A330S/P331S	5.4E−09	2.8E−06	3.1E−05	1.2E−07	8.2E−05	0.059	0.39	0.020	17.5	0.005
IL6R-P768/IL6R-L	P238D/V264I/S267A/P271G/A327G/A330S/P331S	3.3E−09	2.8E−06	2.3E−05	1.7E−07	4.8E−05	0.097	0.39	0.027	12.4	0.008
IL6R-P769/IL6R-L	P238D/V264I/P271G	1.2E−08	2.9E−06	9.5E−05	7.4E−08	6.3E−05	0.027	0.38	0.007	28.4	0.006
IL6R-P770/IL6R-L	P238D/V264I/P271G/A327G/A330S/P331S	1.5E−08	3.4E−06	1.1E−04	1.7E−07	5.2E−05	0.021	0.32	0.006	12.4	0.007
IL6R-P771/IL6R-L	P238D/V264I/P271G/K322A	1.4E−08	4.3E−06	8.5E−05	1.4E−07	3.7E−05	0.023	0.26	0.007	15.0	0.010
IL6R-P772/IL6R-L	P238D/V264I/P271G/K322E	9.1E−09	3.1E−06	9.8E−05	9.7E−08	6.4E−05	0.035	0.35	0.006	21.6	0.006
IL6R-P773/IL6R-L	P238D/S267A/H268E/P271G/K322A	2.1E−09	2.7E−06	9.8E−05	1.4E−07	4.2E−05	0.152	0.41	0.006	15.0	0.009
IL6R-P774/IL6R-L	P238D/S267A/H268E/P271G/K322E	1.1E−09	2.0E−06	7.5E−05	8.8E−08	3.0E−05	0.291	0.55	0.008	23.9	0.012
IL6R-P112/IL6R-L	P238D/P271G	3.5E−09	3.4E−06	3.5E−05	2.8E−07	7.6E−05	0.091	0.32	0.018	7.5	0.005
IL6R-P555/IL6R-L	G237D/P238D/H268D/P271G	1.1E−08	2.8E−06	2.9E−05	2.1E−07	4.0E−05	0.029	0.39	0.021	10.0	0.009
IL6R-P556/IL6R-L	E233D/G237D/P238D/H268D/P271G	3.7E−09	2.4E−06	3.3E−05	1.8E−07	5.5E−05	0.086	0.46	0.019	11.7	0.007
IL6R-P559/IL6R-L	G237D/P238D/H268E/P271G	9.1E−09	2.4E−06	3.0E−05	1.4E−07	4.3E−05	0.035	0.46	0.021	15.0	0.009
IL6R-P562/IL6R-L	E233D/G237D/P238D/H268E/P271G	4.2E−09	2.0E−06	2.8E−05	1.3E−07	5.2E−05	0.076	0.55	0.022	16.2	0.007
IL6R-P763/IL6R-L	P238D/S267A/H268E/P271G	7.3E−10	1.6E−06	1.8E−05	1.0E−07	2.7E−05	0.438	0.69	0.034	21.0	0.014
IL6R-P764/IL6R-L	P238D/V264I/H268E/P271G	3.7E−09	2.1E−06	2.4E−05	5.0E−08	6.1E−05	0.086	0.52	0.026	42.0	0.006
IL6R-P765/IL6R-L	P238D/V264I/S267A/P271G	1.8E−09	2.1E−06	2.2E−05	5.3E−08	4.8E−05	0.178	0.52	0.028	39.6	0.008
IL6R-P775/IL6R-L	P238D/P271G/K322A	8.6E−09	5.4E−06	1.3E−04	4.0E−07	5.0E−05	0.037	0.20	0.005	5.3	0.007
IL6R-P776/IL6R-L	P238D/P271G/K322E	5.2E−09	4.0E−06	9.6E−05	2.8E−07	3.6E−05	0.062	0.28	0.006	7.5	0.010
IL6R-P777/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/K322A	1.6E−09	1.9E−06	7.3E−05	5.2E−08	5.0E−05	0.200	0.58	0.008	40.4	0.007
IL6R-P778/IL6R-L	E233D/P238D/V264I/S267A/H268E/P271G/K322E	9.6E−10	1.3E−06	6.0E−05	2.5E−08	4.1E−05	0.333	0.85	0.010	84.0	0.009
IL6R-P779/IL6R-L	P238D/V264I/S267A/H268E/P271G/K322A	3.4E−09	2.2E−06	7.0E−05	5.0E−08	3.3E−05	0.094	0.50	0.009	42.0	0.011
IL6R-P780/IL6R-L	P238D/V264I/S267A/H268E/P271G/K322E	2.1E−09	1.7E−06	7.8E−05	3.4E−08	4.9E−05	0.152	0.65	0.008	61.8	0.008
IL6R-P781/IL6R-L	P238D/S298A/A327G/A330S/P331S	1.7E−08	1.1E−05	2.2E−04	9.0E−06	5.1E−05	0.019	0.10	0.003	0.2	0.007
IL6R-P782/IL6R-L	E233D/P238D/A327G/A330S/P331S	3.0E−08	3.7E−05	1.3E−04	3.6E−06	6.1E−05	0.011	0.03	0.005	0.6	0.006
IL6R-P783/IL6R-L	G237D/P238D/A327G/A330S/P331S	1.6E−07	3.9E−05	9.8E−05	3.8E−06	5.7E−05	0.002	0.03	0.006	0.6	0.006
IL6R-P784/IL6R-L	E233D/G237D/P238D/A327G/A330S/P331S	8.8E−08	2.1E−05	8.7E−05	2.2E−06	3.8E−05	0.004	0.05	0.007	1.0	0.010
IL6R-P785/IL6R-L	E233D/P238D/S298A/A327G/A330S/P331S	2.3E−08	8.7E−05	9.4E−05	7.9E−06	4.1E−05	0.014	0.01	0.007	0.3	0.009
IL6R-P786/IL6R-L	G237D/P238D/S298A/A327G/A330S/P331S	1.2E−07	6.7E−05	1.0E−04	8.5E−06	3.6E−05	0.003	0.02	0.006	0.2	0.010
IL6R-P787/IL6R-L	E233D/G237D/P238D/S298A/A327G/A330S/P331S	5.6E−08	5.1E−05	8.6E−05	4.6E−06	3.6E−05	0.006	0.02	0.007	0.5	0.010